CN105040505B - A kind of method that aspergillus oryzae whole-cell catalyst handles mthod of white water from paper making resin - Google Patents
A kind of method that aspergillus oryzae whole-cell catalyst handles mthod of white water from paper making resin Download PDFInfo
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- CN105040505B CN105040505B CN201510382996.1A CN201510382996A CN105040505B CN 105040505 B CN105040505 B CN 105040505B CN 201510382996 A CN201510382996 A CN 201510382996A CN 105040505 B CN105040505 B CN 105040505B
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21F—PAPER-MAKING MACHINES; METHODS OF PRODUCING PAPER THEREON
- D21F1/00—Wet end of machines for making continuous webs of paper
- D21F1/66—Pulp catching, de-watering, or recovering; Re-use of pulp-water
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Abstract
The invention discloses a kind of method that use aspergillus oryzae whole-cell catalyst handles mthod of white water from paper making.This method uses different culture mediums first, and aspergillus oryzae is screened, and selects enzyme activity to be tested with respect to highest aspergillus oryzae whole-cell catalyst, and aspergillus oryzae condition of culture is optimized；And obtained aspergillus oryzae whole-cell catalytic will be cultivated in optimal conditions for handling mthod of white water from paper making model thing, by the analysis to aspergillus oryzae whole-cell catalyst to the product of mthod of white water from paper making catalytic degradation, so that it is determined that going out disposition of the aspergillus oryzae whole-cell catalyst to mthod of white water from paper making.Dissolving and colloid the material in mthod of white water from paper making is catalytically decomposed with whole-cell catalysis using aspergillus oryzae by the present invention（DCS）, reduce the cationic demand of plain boiled water（CD）, the adverse effect that mthod of white water from paper making closed circulation is brought is alleviated, the problems such as overcoming the nonrepeatability and high processing cost of common resolvase during White Water Treatment, advantages of environment protection low with cost.
Description
Technical field
The invention belongs to wet end chemistry technical field, and in particular to aspergillus oryzae whole-cell catalyst is white in processing papermaking
Method in water.
Background technology
The reuse of mthod of white water from paper making can not only save the consumption of clear water, reduce the loss of fiber and chemicals, save resource
With reduce cost, reduce environmental pollution and also have great importance.But with the raising of white water system degree, in plain boiled water
Harmful substance also can gradually accumulate.In these harmful substances, maximum, most intractable on wet end chemistry influence is white
Dissolving material and colloidal substance in water（DCS）.DCS is on the one hand from soluble wood extractive（Such as small molecule lignin, shallow lake
Powder, carbohydrate）, the various organic and inorganic additive that adds in natural materials and the water such as resin and production process and application change
Learn medicine.Resinous matter is the hydrophobic substance that some are dissolved in neutral organic solvent in plain boiled water DCS, and this moieties is being made
Equipment surface can be deposited on during paper in a variety of forms, causes to shut down the problems such as with papery declining.
Conventional processing method for problems mainly uses cationic polyelectrolyte to be neutralized, this process
Not only need to consume a large amount of chemicals, and processing cost is high, while the follow-up water process difficulty of papermaking can be increased.Enzyme preparation due to
With efficient, reaction condition is gentle, advantages of environment protection, applied to pulping and papermaking processes to solve bio-pulping, tree
The problems such as fat obstacle, improvement fibre property, bio-bleaching and wastewater treatment.
Due to commercialized enzyme preparation is used at present resolvase during White Water Treatment the enzyme activity retention time compared with
It is short, do not possess repeatability, in addition, processing cost is also high, these all limit enzyme in papermaking engineering large-scale promotion and application.
If directly handle mthod of white water from paper making with whole-cell catalyst, the stabilization of catalyst can be improved, catalyst recycling, will
The processing cost of mthod of white water from paper making can be substantially reduced, with more wide application prospect., can be to meter Qu due to the difference of culture medium
Mould activity and purposes produce considerable influence.The aspergillus oryzae that the present invention is induced using Tween 80 is as whole-cell catalyst to papermaking
Plain boiled water is handled, and the optimal treatment condition of aspergillus oryzae whole-cell catalyst is carried out using mthod of white water from paper making resin mould thing
Research.
The content of the invention
In order in place of overcoming the shortcoming and defect of above-mentioned prior art, the invention provides one kind it is environment-friendly, be produced into
The method that the simple controllable aspergillus oryzae whole-cell catalyst of this low, course of reaction handles mthod of white water from paper making.
The purpose of the present invention is achieved through the following technical solutions.
A kind of method that use aspergillus oryzae whole-cell catalyst handles mthod of white water from paper making, comprises the following steps：
（1）The screening of aspergillus oryzae, aspergillus oryzae 35 is induced using Tween 80；
（2）By step（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35 is used to handle mthod of white water from paper making resin mould thing.
Further, step（1）Use Tween 80 induction aspergillus oryzae 35 is specifically：With containing 0.1% ~ 1.5% (NH4)2SO4, 0.1% ~ 1.0% Tween 80,0.01% ~ 0.2% yeast extract (Yeast extract), 0.01% ~ 0.1% K2HPO4、0.01~
0.05% MgSO4·7H2O culture medium, cultivation and fermentation aspergillus oryzae 35；The calculating benchmark of the percentage is to account for culture medium
Gross mass meter.
Further, the lipase active can be completely applied to the processing requirement of mthod of white water from paper making up to 100 ~ 300U/g.
Further, the plain boiled water resin mould thing is olein or lignin fatty acid ester, or resin acid, glycerine
Sour fat or unsaponifiable matter.
Further, step（2）Specifically：Mthod of white water from paper making resin mould thing is added in the phosphate buffer that PH is 7 ~ 8,
Then step is added（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35, is 20 ~ 120min of lower reaction at 20 ~ 70 DEG C, instead
It is 30 ~ 50% to answer concentration of substrate in system（The volume ratio of olein and phosphate buffer）.
The present invention can effectively degrade the lipids such as olein, aliphatic acid, resin acid, glyceric acid fat and some not soaps
The object module thing such as compound, is catalytically decomposed as corresponding acid.The oleic acid of generation is titrated using NaOH titer titration, is sentenced
Medium well is into sour amount, so as to be inferred to decomposition situation of the aspergillus oryzae to object module thing.
The present invention principle be：The chemical composition of resin mainly has the stationeries such as aliphatic acid, resin acid, glyceric acid fat and some
Unsaponifiable matter etc..Lipid material in resin can be hydrolyzed into resin acid and alcohols of low stickiness etc. by lipase, be mitigated or eliminated
The problem on obstacle of resin.
Compared with prior art, the present invention has advantages below and beneficial effect：
（1）Olein is catalytically decomposed using the whole-cell catalyst of efficient, high stability in the method for the present invention,
Overcome enzyme in the prior art easily to inactivate, the low shortcoming of reaction efficiency；
（2）Reaction condition is gentle, environment-friendly；
（3）Whole-cell catalyst aspergillus oryzae production needed for reaction is simple, cheaply, greatly reduces production cost, improves
Production efficiency.
（4）The yield of aspergillus oryzae is more considerable compared to other bacterial fungus class cells, and this is also further industrial applicability
Improving may.
Brief description of the drawings
Fig. 1 is the result that reaction temperature aspergillus oryzae is catalytically decomposed olein.
Fig. 2 is the result that PH aspergillus oryzae is catalytically decomposed olein.
Fig. 3 is the result that concentration of substrate aspergillus oryzae is catalytically decomposed olein.
Embodiment
To be best understood from the present invention, the present invention will be described in further detail with reference to the following examples, but of the invention
Claimed scope is not limited to the scope that embodiment is represented.
The screening of aspergillus oryzae, due to the difference of culture medium, can produce considerable influence to the activity and purposes of aspergillus oryzae, use
Glucose induction and Tween 80 induce two kinds of different culture medias, and two kinds of aspergillus oryzaes 3.4862, aspergillus oryzae 3.5232（It is purchased from
Institute of Microorganism, Academia Sinica）, the lipase active for comparing 4 kinds of different aspergillus oryzaes is co-cultured, lipase active is selected
Tested with respect to highest aspergillus oryzae.The lipase active highest of aspergillus oryzae 35 of gained under Tween 80 inductive condition, can be with
Resin deposit processing requirement applied to mthod of white water from paper making.So the aspergillus oryzae 35 that the present invention is induced using Tween 80 is as complete thin
Born of the same parents' catalyst handles mthod of white water from paper making resin deposit model.
Embodiment 1
A kind of method that aspergillus oryzae handles mthod of white water from paper making resin, comprises the following steps：
(1) primary election of aspergillus oryzae.By Tween 80 condition of culture be 0.5% (NH4)2SO4, 0.5% Tween 80,0.1% yeast
Medicinal extract (Yeast extract), 0.1% K2HPO4, 0.02% MgSO4·7H2O seed culture mediums, the aspergillus oryzae of induction is as complete
Cell catalyst handles mthod of white water from paper making resin deposit model（Triglyceride, similarly hereinafter）.
（2）By step（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35 is used to handle mthod of white water from paper making resin mould thing.
Weigh 15mL oleins to be added in the 15mL phosphate buffers that PH is 7.5,0.3g aspergillus oryzaes are added after being well mixed
The lipase of fermenting and producing, in 45 DEG C of water-baths after accurate reaction 60min, takes 6mL samples to be added in 15mL95% ethanol eventually
Only react, pH10.3 is then titrated on potentiometric titrimeter for terminal using 0.005mol/L NaOH titer titrations.According to
The volume of the NaOH titer titration consumed is titrated to judge decomposition situation of the aspergillus oryzae to olein.The present embodiment
Olein is catalytically decomposed in aspergillus oryzae, and the degradation rate of olein is shown in Table 1.
Embodiment 2
A kind of method that aspergillus oryzae handles mthod of white water from paper making resin, comprises the following steps：
(1) primary election of aspergillus oryzae.By Tween 80 condition of culture be 0.5% (NH4)2SO4, 0.5% Tween 80,0.1% yeast
Medicinal extract (Yeast extract), 0.1% K2HPO4, 0.02% MgSO4·7H2O seed culture mediums, the aspergillus oryzae of induction is as complete
Cell catalyst handles mthod of white water from paper making resin deposit model.
（2）By step（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35 is used to handle mthod of white water from paper making resin mould thing.
Weigh 15mL oleins to be added in the 15mL phosphate buffers that PH is 9.0,0.3g aspergillus oryzaes are added after being well mixed
The lipase of fermenting and producing, in 45 DEG C of water-baths after accurate reaction 30min, takes 6mL samples to be added in 15mL95% ethanol eventually
Only react, pH10.3 is then titrated on potentiometric titrimeter for terminal using 0.005mol/L NaOH titer titrations.According to
The volume of the NaOH titer titration consumed is titrated to judge decomposition situation of the aspergillus oryzae to olein.The present embodiment
Olein is catalytically decomposed in aspergillus oryzae, and the degradation rate of olein is shown in Table 1.
Embodiment 3
A kind of method that aspergillus oryzae handles mthod of white water from paper making resin, comprises the following steps：
(1) primary election of aspergillus oryzae.By Tween 80 condition of culture be 0.5% (NH4)2SO4, 0.5% Tween 80,0.1% yeast
Medicinal extract (Yeast extract), 0.1% K2HPO4, 0.02% MgSO4·7H2O seed culture mediums, the aspergillus oryzae of induction is as complete
Cell catalyst handles mthod of white water from paper making resin deposit model.
（2）By step（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35 is used to handle mthod of white water from paper making resin mould thing.
Weigh 5mL oleins to be added in the 25mL phosphate buffers that PH is 7.5,0.3g aspergillus oryzaes hair is added after being well mixed
The lipase of ferment production, in 45 DEG C of water-baths after accurate reaction 30min, takes 6mL samples to be added in 15mL95% ethanol and terminates
Reaction, right, how many building are titrated to pH10.3 on potentiometric titrimeter for terminal.According to the sodium hydroxide mark of titration consumption
The volume of quasi- liquid judges decomposition situation of the aspergillus oryzae to olein.Three oleic acid are catalytically decomposed in the aspergillus oryzae of the present embodiment
Glyceride, the degradation rate of olein is shown in Table 1.
Embodiment 4
A kind of method that aspergillus oryzae handles mthod of white water from paper making resin, comprises the following steps：
(1) primary election of aspergillus oryzae.By Tween 80 condition of culture be 0.5% (NH4)2SO4, 0.5% Tween 80,0.1% yeast
Medicinal extract (Yeast extract), 0.1% K2HPO4, 0.02% MgSO4·7H2O seed culture mediums, the aspergillus oryzae of induction is as complete
Cell catalyst handles mthod of white water from paper making resin deposit model.
（2）By step（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35 is used to handle mthod of white water from paper making resin mould thing.
Weigh 25mL oleins to be added in the 5mL phosphate buffers that PH is 7.5,0.3g aspergillus oryzaes hair is added after being well mixed
The lipase of ferment production, in 45 DEG C of water-baths after accurate reaction 10min, takes 6mL samples to be added in 15mL95% ethanol and terminates
Reaction, is then titrated to pH10.3 for terminal using 0.005mol/L NaOH titer titrations on potentiometric titrimeter.According to drop
Surely the volume of the NaOH titer titration consumed judges decomposition situation of the aspergillus oryzae to olein.The rice of the present embodiment
Olein is catalytically decomposed in aspergillus, and the degradation rate of olein is shown in Table 1.
Embodiment 5
A kind of method that aspergillus oryzae handles mthod of white water from paper making resin, comprises the following steps：
(1) primary election of aspergillus oryzae.By Tween 80 condition of culture be 0.5% (NH4)2SO4, 0.5% Tween 80,0.1% yeast
Medicinal extract (Yeast extract), 0.1% K2HPO4, 0.02% MgSO4·7H2O seed culture mediums, the aspergillus oryzae of induction is as complete
Cell catalyst handles mthod of white water from paper making resin deposit model.
（2）By step（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35 is used to handle mthod of white water from paper making resin mould thing.
Weigh 15mL oleins to be added in the 15mL phosphate buffers that PH is 8.5,0.3g aspergillus oryzaes are added after being well mixed
The lipase of fermenting and producing, in 45 DEG C of water-baths after accurate reaction 120min, takes 6mL samples to be added in 15mL95% ethanol
Terminating reaction, is then titrated to pH10.3 for terminal using 0.005mol/L NaOH titer titrations on potentiometric titrimeter.Root
Judge decomposition situation of the aspergillus oryzae to olein according to the volume of the NaOH titer titration of titration consumption.The present embodiment
Aspergillus oryzae catalytic decomposition olein, the degradation rate of olein is shown in Table 1.
Embodiment 6
A kind of method that aspergillus oryzae handles mthod of white water from paper making resin, comprises the following steps：
(1) primary election of aspergillus oryzae.By Tween 80 condition of culture be 0.5% (NH4)2SO4, 0.5% Tween 80,0.1% yeast
Medicinal extract (Yeast extract), 0.1% K2HPO4, 0.02% MgSO4·7H2O seed culture mediums, the aspergillus oryzae of induction is as complete
Cell catalyst handles mthod of white water from paper making resin deposit model.
（2）By step（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35 is used to handle mthod of white water from paper making resin mould thing.
Weigh 15mL oleins to be added in the 15mL phosphate buffers that PH is 8.5,0.3g aspergillus oryzaes are added after being well mixed
The lipase of fermenting and producing, in 65 DEG C of water-baths after accurate reaction 60min, takes 4mL samples to be added in 15mL95% ethanol eventually
Only react, pH10.3 is then titrated on potentiometric titrimeter for terminal using 0.005mol/L NaOH titer titrations.According to
The volume of the NaOH titer titration consumed is titrated to judge decomposition situation of the aspergillus oryzae to olein.The present embodiment
Olein is catalytically decomposed in aspergillus oryzae, and the degradation rate of olein is shown in Table 1.
Embodiment 7
A kind of method that aspergillus oryzae handles mthod of white water from paper making resin, comprises the following steps：
(1) primary election of aspergillus oryzae.By Tween 80 condition of culture be 0.5% (NH4)2SO4, 0.5% Tween 80,0.1% yeast
Medicinal extract (Yeast extract), 0.1% K2HPO4, 0.02% MgSO4·7H2O seed culture mediums, the aspergillus oryzae of induction is as complete
Cell catalyst handles mthod of white water from paper making resin deposit model.
（2）By step（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35 is used to handle mthod of white water from paper making resin mould thing.
Weigh 15mL oleins to be added in the 15mL phosphate buffers that PH is 8.5,0.3g aspergillus oryzaes are added after being well mixed
The lipase of fermenting and producing, in 55 DEG C of water-baths after accurate reaction 15min, takes 4mL samples to be added in 15mL95% ethanol eventually
Only react, pH10.3 is then titrated on potentiometric titrimeter for terminal using 0.005mol/L NaOH titer titrations.According to
The volume of the NaOH titer titration consumed is titrated to judge decomposition situation of the aspergillus oryzae to olein.The present embodiment
Olein is catalytically decomposed in aspergillus oryzae, and the degradation rate of olein is shown in Table 1.
The result of olein is catalytically decomposed in the embodiment 1-7 aspergillus oryzaes of table 1
| Sample | Concentration of substrate | Temperature | PH | Reaction time | Degradation rate |
| Embodiment 1 | 50% | 45 | 7.5 | 60min | 94.8% |
| Embodiment 2 | 50% | 45 | 9.0 | 30min | 43.5% |
| Embodiment 3 | 16.7% | 45 | 7.5 | 30min | 50.9% |
| Embodiment 4 | 83.3% | 45 | 7.5 | 10min | 37.9% |
| Embodiment 5 | 50% | 45 | 8.5 | 120min | 73.8% |
| Embodiment 6 | 50% | 65 | 8.5 | 60min | 38.2% |
| Embodiment 7 | 50% | 55 | 8.5 | 15min | 45.7% |
As it can be seen from table 1 the present invention handles mthod of white water from paper making resin mould thing, the drop of olein using aspergillus oryzae
Solution rate is very high, thus infers that plain boiled water resin mould thing is decomposed.
Above-described embodiment is not limited for the present invention preferably embodiment, but embodiments of the present invention by above-described embodiment
System, other any Spirit Essences without departing from the present invention and the modification made under principle, changes, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (3)
1. a kind of method that use aspergillus oryzae whole-cell catalyst handles mthod of white water from paper making, it is characterised in that comprise the following steps：
（1）The screening of aspergillus oryzae, aspergillus oryzae 35 is induced using Tween 80, and the use Tween 80 induction aspergillus oryzae 35 is specifically：
With containing 0.1% ~ 1.5% (NH4)2SO4, 0.1% ~ 1.0% Tween 80,0.01% ~ 0.2% yeast extract (Yeast extract),
0.01%~0.1% K2HPO4、0.01~0.05% MgSO4·7H2O culture medium, cultivation and fermentation aspergillus oryzae 35；The calculating of percentage
Benchmark is in terms of the gross mass for accounting for culture medium；
（2）By step（1）The lipase of the obtained fermenting and producing of aspergillus oryzae 35 is used to handle mthod of white water from paper making resin mould thing, specifically
It is：Mthod of white water from paper making resin mould thing is added pH in 7 ~ 8 phosphate buffer, then to add step（1）Obtained meter Qu
The lipase of mould 35 fermenting and producing, reacts 20 ~ 120min at 20 ~ 70 DEG C, in reaction system mthod of white water from paper making resin mould thing with
The volume ratio of phosphate buffer is 30 ~ 50%.
2. the method that a kind of use aspergillus oryzae whole-cell catalyst according to claim 1 handles mthod of white water from paper making, its feature
It is, the lipase active fully meets the processing requirement of mthod of white water from paper making up to 100 ~ 300U/g.
3. the method that a kind of use aspergillus oryzae whole-cell catalyst according to claim 1 handles mthod of white water from paper making, its feature
It is, the plain boiled water resin mould thing is olein, lignin fatty acid ester, resin acid, glyceric acid fat or unsaponifiable matter.
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| CN101418287B (en) * | 2008-11-28 | 2010-12-08 | 天津科技大学 | Aspergillus niger liquid state fermentation pectic enzyme and control to colloidal matter in white water and paper stuff |
| CN103074795B (en) * | 2012-12-26 | 2015-04-29 | 泰兴市一鸣生物制品有限公司 | Method for controlling secondary stickies through lipase |
| CN103061184A (en) * | 2012-12-26 | 2013-04-24 | 泰兴市一鸣生物制品有限公司 | Method of controlling secondary gummy substances by using lipase |
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