CN106480771B - A method of utilizing cutin enzymatic treatment mthod of white water from paper making - Google Patents
A method of utilizing cutin enzymatic treatment mthod of white water from paper making Download PDFInfo
- Publication number
- CN106480771B CN106480771B CN201611203776.9A CN201611203776A CN106480771B CN 106480771 B CN106480771 B CN 106480771B CN 201611203776 A CN201611203776 A CN 201611203776A CN 106480771 B CN106480771 B CN 106480771B
- Authority
- CN
- China
- Prior art keywords
- cutinase
- water
- plain boiled
- boiled water
- mthod
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21F—PAPER-MAKING MACHINES; METHODS OF PRODUCING PAPER THEREON
- D21F1/00—Wet end of machines for making continuous webs of paper
- D21F1/66—Pulp catching, de-watering, or recovering; Re-use of pulp-water
Abstract
The invention discloses a kind of methods using cutin enzymatic treatment mthod of white water from paper making, belong to technical field of enzyme engineering.Method of the invention decomposes olein using the cutinase enzymatic of efficient stable, and the lipids such as polyvinyl acetate, without being heated at high temperature or being vigorously stirred, reaction condition is mild, and degradation rate is environmental-friendly up to 83.5%.Cutinase production needed for reaction is simple, improves production efficiency.Meanwhile enzymatic treatment compares other physical treatments, step is simple, in practical plain boiled water treatment process, also has a preferable treatment effect when enzyme concentration is 0.5~1U/g substrate, and turbidity difference is up to 95% or more.Plain boiled water after cutinase is processed can partly or entirely substitute water for cleaning with direct reuse, to realize the closed cycle of paper manufacturing systems water, reach zero-emission truly.The today's society that these advantages are paid more and more attention in environmental protection, it appears particularly important.
Description
Technical field
The present invention relates to a kind of methods using cutin enzymatic treatment mthod of white water from paper making, belong to technical field of enzyme engineering.
Background technique
Worldwide, the waste water of paper industry is all the pollution sources being concerned.In China, paper waste pollutes
As the key factor for restricting industry survival and development.Paper-making effluent mainly includes that black liquor, middle section water, mthod of white water from paper making three are big
Class.After pulping system is with cleaning procedure such as " alkaline hydrogen peroxide cooking-bleaching integration " substitution soda processes traditional handicraft, slurrying
Lower (the < 60m of wastewater discharge3/ ton slurry), pollutional load is equivalent to 50% of traditional handicraft or so, advanced slurrying
Paper manufacturing systems only discharge steaming drift waste water and mthod of white water from paper making.Compared with steaming and floating waste water, mthod of white water from paper making mainly contains fiber fines, miscellaneous thin
Born of the same parents' object, chemical additive (such as sizing material, filler, chemical assistant), ingredient is relatively easy, is that most recycling can in paper waste
A part of energy property.
The reuse of mthod of white water from paper making can not only save the dosage of clear water, reduce the loss of fiber and chemicals, save resource
With reduce cost, reduce environmental pollution and also have great importance.But with the raising of white water system degree, in plain boiled water
Harmful substance also can gradually accumulate.In these harmful substances, wet end chemistry is influenced maximum, most intractable to be white
Dissolution substance and colloidal substance (DCS) in water.On the one hand DCS derives from soluble wood extractive (such as small molecule lignin forms sediment
Powder, 7 cap classes), the various organic and inorganic additive that adds in natural materials and the water such as resin and production process and application
Chemicals.Resinous matter is some hydrophobic substances for being dissolved in neutral organic solvent in plain boiled water DCS, this moieties is being made
It can be deposited on equipment surface in a variety of forms during paper, cause the problems such as shutting down with papery decline.
It is mainly neutralized using cationic polyelectrolyte for the common processing method of problems, this process
Not only need to consume a large amount of chemicals, and processing cost is high, while will increase the subsequent water process difficulty of papermaking, therefore, exploitation
A kind of mild, the environmental-friendly White Water Treatment method of reaction condition is very necessary.
Summary of the invention
To solve the above-mentioned problems, the present invention provides it is a kind of it is environmental-friendly, production cost is low, reaction process is simply controllable
Cutin enzymatic treatment mthod of white water from paper making method.Treated, and plain boiled water can satisfy different reuse requirements, reach mthod of white water from paper making zero
The purpose of discharge.
The first purpose of the invention is to provide a kind of method of resinous matter in degradation mthod of white water from paper making, the method is
Cutinase is added with the additive amount of 0.5~8000U/g substrate into the solution of resinous substance, control processing time 30-
240min, pH are that 7-9 carries out enzymolysis processing；The resinous matter includes olein, lignin aliphatic ester, resin
Acid, polyvinyl acetate.
In one embodiment of the invention, the resinous matter is olein, lignin aliphatic ester, tree
The combination of one or more of resin acid, polyvinyl acetate.
In one embodiment of the present invention, the concentration of the resinous matter is 4~400g/L.
In one embodiment of the invention, the resinous matter is three oleics of 200~400g/L of concentration
Ester handles 30~60min of time, controls 40~60 DEG C of temperature.
In one embodiment of the invention, the resinous matter is the polyvinyl acetate of 5~2g/L of concentration, place
120~180min of time is managed, controls 30~50 DEG C of temperature.
In one embodiment of the invention, the resinous matter is three oleics that total concentration is 5~20g/L
Ester, lignin aliphatic ester, resin acid, polyacrylic acid ester admixture handle 120~240min of time.
In one embodiment of the invention, the concentration of polyvinyl acetate is 5~20g/L.
In one embodiment of the invention, the mthod of white water from paper making also contains, fiber fines, filler, coating and dissolution
Wood component, and the white in appearance milkiness state such as sizing material, wet strength agent, preservative of addition is tasteless, not easily settled；
In one embodiment of the invention, the cutinase comes from thermophilic sporangium Thermobifida fusca
Or amino acid sequence with derive from Thermobifida fusca cutinase at least 60%, especially 65%, more precisely
70% or 75% or 80% or 85% or 90% or 92% or 95% or 97%, or at least 99% homology and tool
There is the enzyme of Cutinase activity.
In one embodiment of the invention, the cutinase also comes from Fusarium solani Fusarium solani
Or amino acid sequence with derive from Fusarium solani cutinase at least 60%, especially 65%, more precisely
70% or 75% or 80% or 85% or 90% or 92% or 95% or 97%, or at least 99% homology and tool
There is the enzyme of Cutinase activity.
In one embodiment of the invention, the cutinase also comes from Humicola insolens Humicola
Insolens or amino acid sequence and the cutinase at least 60%, especially 65% for deriving from Humicola Insolens, it is more quasi-
Really say 70% or 75% or 80% or 85% or 90% or 92% or 95% or 97%, or at least 99% it is homologous
Property and with Cutinase activity enzyme.
In one embodiment of the invention, it is AAZ54920, AAZ54921 that the cutinase, which is Genbank accession number,
Or the cutinase of AAA33334.
In one embodiment of the invention, the cutinase is the cutinase that PDB accession number is 4OYY.
In one embodiment of the invention, the cutinase is fermented by the recombination bacillus coli of production cutinase
24-48h is centrifuged 10min by 12000rpm, removes thallus, collects the cutinase crude enzyme liquid that supernatant obtains；The recombination is big
Enterobacteria is disclosed in Identification and Characterization of Bacterial Cutinase, The
Journal of Biological Chemistry,2008,283(28)25854-25862。
In one embodiment of the invention, the production method of the cutinase be also under certain condition of culture, with
The recombinant yeast pichia pastoris for producing cutinase ferments certain time, by 12000rpm, is centrifuged 10min, removes thallus, supernatant is
Crude enzyme liquid.
In one embodiment of the invention, it is carrier that the recombinant yeast pichia pastoris, which is pPIC9K, and expression Genbank is stepped on
Record number is the recombinant yeast pichia pastoris for the cutinase that AAA33334 or Protein data bank accession number is 4OYY.
In one embodiment of the invention, it is carrier that the recombinant yeast pichia pastoris, which is pPIC9K, expresses PDB accession number
For the recombinant yeast pichia pastoris of the cutinase of 4OYY.
In one embodiment of the invention, the fermentation is that the recombinant yeast pichia pastoris is seeded to fermentation medium
In, 28~37 DEG C of fermentations.
A second object of the present invention is to provide the methods in field of industrial waste water treatment, especially pulping wastewater treatment
The application of aspect.
The utility model has the advantages that method of the invention decomposes olein, polyvinyl acetate using the enzymatic of efficient stable
The lipids such as ester, without being heated at high temperature or being vigorously stirred, reaction condition is mild, and degradation rate is environmental-friendly up to 83.5%.Reaction institute
The cutinase production needed is simple, improves production efficiency.Meanwhile enzymatic treatment compares other physical treatments, step is simple, in reality
In the plain boiled water treatment process of border, also there is preferable treatment effect when enzyme concentration is 0.5~1U/g substrate, turbidity difference up to 95% with
On.Plain boiled water after cutinase is processed can partly or entirely substitute water for cleaning with direct reuse, to realize paper manufacturing systems
With the closed cycle of water, reach zero-emission truly.The current society that these advantages are paid more and more attention in environmental protection
Meeting, it appears particularly important.
Specific embodiment
The enzyme activity determination method of cutinase:
At 37 DEG C, enzyme activity is measured using continuous spectrophotometry.Reaction total volume is 1.5mL, including 30 μ L enzyme solutions
It is buffered with the Tris-HCl of 1470 μ L sulphur containing 50mmol/L NaTDC and 50mmol/L p-nitrophenyl butyrate (pNPB)
Liquid (pH 8.0) records the generating rate of paranitrophenol at wavelength 405nm.Enzyme activity is defined as: at 37 DEG C, per minute will
The enzyme amount that p-nitrophenyl butyrate catalyzing hydrolysis generates 1 μm of ol paranitrophenol is an enzyme activity unit.
(three oleic acid are sweet for the cutin enzyme hydrolysis mthod of white water from paper making resin deposit in the source embodiment 1:Thermobifida fusca
Grease) model
Weighing 15mL olein, (0.915g/mL is added in the Tris-HCl buffer 85mL of pH8.0, mixing
Take 10mL that the source 100U Thermobifida fusca cutinase (Genbank accession number: AAZ54920) is added after uniformly,
After accurately reacting 30min in 60 DEG C of water-baths, the ethyl alcohol for taking 5mL reaction solution that 5mL95% is added terminates reaction, then utilizes
It is terminal that 0.05mol/L NaOH titer titration is titrated to pH 10.3 on potentiometric titrimeter.According to the hydroxide of titration consumption
The volume of sodium titer judges the cutinase to the decomposition situation of olein.Cutinase can decompose olein,
Oleic acid is generated, by titration, consumption sodium hydroxide concentration is more, illustrates that enzyme reaction is more thorough.The results are shown in Table 1, works as cutin
When enzyme additive amount is 72.86U/g substrate reactions liquid, after 60 DEG C of reaction 30min, olein degradation rate is up to 83.5%.
The cutin enzyme hydrolysis olein in 1 source Thermobifida fusca of table
Cutin enzyme hydrolysis mthod of white water from paper making resin deposit (three oleics in the source embodiment 2:Fusarium solani
Ester) model
It weighs 15mL olein to be added in the Tris-HCl buffer 85mL of pH8.5, take after mixing
The source 1000U Fusarium solani cutinase (Genbank accession number: AAA33334) is added in 10mL, in 60 DEG C of water-baths
After accurate reaction 60min, the ethyl alcohol for taking 5mL sample that 5mL 95% is added terminates reaction, then utilizes 0.05mol/L sodium hydroxide
It is terminal that titer is titrated to pH10.3 on potentiometric titrimeter.Sentenced according to the volume of the NaOH titer titration of titration consumption
Decomposition situation of the disconnected cutinase to olein.The results are shown in Table 2, when cutinase additive amount is 728.6U/g substrate
When reaction solution, after 40 DEG C of reaction 60min, olein degradation rate is up to 74.8%.
The cutin enzyme hydrolysis olein in 2 source Fusarium solani of table
(three oleic acid are sweet for the cutin enzyme hydrolysis mthod of white water from paper making resin deposit in the source embodiment 3:Humicola Insolens
Grease) model
It weighs 15mL olein to be added in the Tris-HCl buffer 85mL of pH8.5, take after mixing
The source 200U Humicola Insolens cutinase (RCSB Protein data bank accession number: 4OYY) is added in 10mL,
After accurately reacting 60min in 40 DEG C of water-baths, the ethyl alcohol for taking 5mL sample that 5mL 95% is added terminates reaction, then utilizes
It is terminal that 0.05mol/L NaOH titer titration is titrated to pH10.3 on potentiometric titrimeter.According to the hydroxide of titration consumption
The volume of sodium titer judges the cutinase to the decomposition situation of olein.The results are shown in Table 3, when cutinase adds
When amount is the reaction solution of 145.72U/g substrate, after 60 DEG C of reaction 60min, olein degradation rate is up to 83.5%.
The cutin enzyme hydrolysis olein in 3 source Humicola Insolens of table
Cutin enzyme hydrolysis mthod of white water from paper making resin deposit (the poly-vinegar acid second in the source embodiment 4:Thermobifida fusca
Enester) model
It weighs 1mL polyvinyl acetate (about 1g/mL) lotion to be added in the Tris-HCl buffer 99mL of pH 8.0, mix
Close uniformly after take 10mL be added the source 200U Thermobifida fusca cutinase (Genbank accession number:
AAZ54920), after accurately reacting 120min in 50 DEG C of water-baths, the ethyl alcohol for taking 5mL sample that 5mL95% is added terminates reaction, so
It is terminal that pH 10.3 is titrated on potentiometric titrimeter using 0.05mol/L NaOH titer titration afterwards.According to titration consumption
The volume of NaOH titer titration judges the cutinase to the decomposition situation of polyvinyl acetate.The results are shown in Table 4, works as cutin
Enzyme additive amount is 200U/g substrate reactions liquid, after 50 DEG C of reaction 120min, consumes 5.0mL sodium hydroxide.
4 Thermobifida fusca cutinase hydrolysed polyvinyl acetate of table
The cutin enzyme hydrolysis mthod of white water from paper making resin deposit (polyvinyl acetate in the source embodiment 5:Fusarium solani
Ester) model
It weighs 1mL polyvinyl acetate emulsion to be added in the Tris-Hcl buffer 99mL of pH8.5, take after mixing
The source 800U Fusarium solani cutinase (Genbank accession number: AAA33334) is added in 10mL, smart in 30 DEG C of water-baths
Really after reaction 180min, the ethyl alcohol for taking 5mL sample that 5mL95% is added terminates reaction, then utilizes 0.05mol/L sodium hydroxide mark
It is terminal that quasi- liquid is titrated to pH10.3 on potentiometric titrimeter.Judged according to the volume of the NaOH titer titration of titration consumption
Decomposition situation of the cutinase to polyvinyl acetate.The results are shown in Table 5.When cutinase additive amount is 8000U/g substrate reactions
Liquid consumes 4.1mL sodium hydroxide after 30 DEG C of reaction 180min
5 Fusarium solani cutinase hydrolysed polyvinyl acetate of table
Cutin enzyme hydrolysis mthod of white water from paper making resin deposit (the poly-vinegar acid second in the source embodiment 6:Humicola Insolens
Enester) model
It weighs 1mL polyvinyl acetate (about 1g/mL) lotion to be added in the Tris-HCl buffer 99mL of pH 8.5, mix
Take 10mL that the cutinase (PDB accession number: 4OYY) in the source 400U Humicola Insolens is added after closing uniformly, in 60 DEG C of water
After accurately reacting 120min in bath, the ethyl alcohol for taking 5mL sample that 5mL95% is added terminates reaction, then utilizes 0.05mol/L hydrogen-oxygen
Changing sodium titer to be titrated to pH 10.3 on potentiometric titrimeter is terminal.According to the body of the NaOH titer titration of titration consumption
Product is to judge cutinase to the decomposition situation of polyvinyl acetate.The results are shown in Table 6, when cutinase additive amount is the bottom 400U/g
Object reaction solution consumes 4.6mL sodium hydroxide after 60 DEG C of reaction 120min.
6 Humicola Insolens cutinase hydrolysed polyvinyl acetate of table
The cutin enzyme hydrolysis mthod of white water from paper making resin deposit mixed model in the source embodiment 7:Thermobifida fusca
It weighs 15mL olein and 1mL polyvinyl acetate emulsion is added to the Tris-HCl buffer of pH8.0
In 84mL, take after mixing 10mL be added the source 200U Thermobifida fusca cutinase (Genbank accession number:
AAZ54920), after accurately reacting 45min in 60 DEG C of water-baths, the ethyl alcohol for taking 5mL reaction solution that 5mL95% is added terminates reaction, so
It is terminal that pH 10.3 is titrated on potentiometric titrimeter using 0.05mol/L NaOH titer titration afterwards.According to titration consumption
The volume of NaOH titer titration judges the cutinase to the decomposition situation of mixed model.The results are shown in Table 7, when cutinase adds
When dosage is 84U/g substrate, after 60 DEG C of reaction 45min, mixed model degradation rate is up to 76.8%.
The cutin enzyme hydrolysis mixed model in 7 source Thermobifida fusca of table
The cutin enzyme hydrolysis mthod of white water from paper making resin deposit mixed model in the source embodiment 8:Fusarium solani
It weighs 15mL olein and 1mL polyvinyl acetate emulsion is added to the Tris-HCl buffer of pH8.5
In 84mL, take after mixing 10mL be added the source 500U Fusarium solani cutinase (Genbank accession number:
AAZ54920), after accurately reacting 60min in 35 DEG C of water-baths, the ethyl alcohol for taking 5mL reaction solution that 5mL95% is added terminates reaction, so
It is terminal that pH 10.3 is titrated on potentiometric titrimeter using 0.05mol/L NaOH titer titration afterwards.According to titration consumption
The volume of NaOH titer titration judges the cutinase to the decomposition situation of mixed model.The results are shown in Table 8, when cutinase adds
When dosage is 210.75U/g, after 35 DEG C of reaction 60min, mixed model degradation rate is up to 66.8%.
The cutin enzyme hydrolysis mixed model in 8 source Thermobifida fusca of table
The cutin enzyme hydrolysis mthod of white water from paper making resin deposit mixed model in the source embodiment 9:Humicola Insolens
It weighs 15mL olein and 1mL polyvinyl acetate emulsion is added to the Tris-HCl buffer of pH8.5
In 84mL, take 10mL that the source 300U Humicola Insolens cutinase (PDB accession number: 4OYY) is added after mixing,
After accurately reacting 60min in 60 DEG C of water-baths, the ethyl alcohol for taking 5mL reaction solution that 5mL95% is added terminates reaction, then utilizes
It is terminal that 0.05mol/L NaOH titer titration is titrated to pH 10.3 on potentiometric titrimeter.According to the hydroxide of titration consumption
The volume of sodium titer judges the cutinase to the decomposition situation of mixed model.The results are shown in Table 9, when cutinase additive amount is
When 126.05U/g, after 35 DEG C of reaction 60min, mixed model degradation rate is up to 73.5%.
The cutin enzyme hydrolysis mixed model in 9 source Thermobifida fusca of table
The cutin enzymatic treatment paper plant secondary cycle residue plain boiled water in the source embodiment 10:Thermobifida fusca
1, water and water quality are handled:
Handle water: 1000m3
Influent quality: 50~200mg/L of COD, pH 7.0,200~1000NTU of turbidity, wherein olein, wood
Plain aliphatic ester, resin acid, polyacrylate total concentration range about 10~20g/L.
2, treatment process:
Plain boiled water pH to 8.5 ± 0.2 is adjusted, cutinase (the Genbank login in the source Thermobifida fusca is added
Number: AAZ54921) 10000U (enzyme concentration about 0.5~1U/g) is handled 3 hours under room temperature (16~25 DEG C).
Water quality after measurement processing, the results are shown in Table 10, and plain boiled water turbidity is reduced to 12NTU by 245, and turbidity difference reaches
95.1%.
10 Thermobifida fusca cutinase hydrolysed residual plain boiled water of table
The cutin enzymatic treatment paper plant secondary cycle residue plain boiled water in the source embodiment 11:Fusarium solani
1, water and water quality are handled:
Handle water: 1500m3
Influent quality: 50~400mg/L of COD, pH 7.0,200~1000NTU of turbidity.
2, treatment process:
Adjust plain boiled water pH to 8.0 ± 0.2, be added the source Fusarium solani cutinase (Genbank accession number:
AAA33334) 80000U (enzyme concentration about 2.67~5.33U/g) is handled 4 hours under room temperature (16~25 DEG C).
Water quality after measurement processing, as a result as shown in table 11, plain boiled water turbidity is reduced to 42NTU by 592, and turbidity difference reaches
92.9%.
The cutinase hydrolysed residual plain boiled water in 11 source Fusarium solani of table
The cutin enzymatic treatment paper plant secondary cycle residue plain boiled water in the source embodiment 12:Humicola Insolens
1, water and water quality are handled:
Handle water: 1500m3
Influent quality: 50~400mg/L of COD, pH 7.0,200~1000NTU of turbidity.
2, treatment process:
Adjust plain boiled water pH to 8.0 ± 0.2, be added the source Humicola Insolens cutinase (PDB accession number:
4OYY) 50000U (enzyme concentration about 2.5~5U/g) is handled 4 hours under room temperature (16~25 DEG C).
Water quality after measurement processing, as a result as shown in table 12, plain boiled water turbidity is reduced to 29NTU by 332, and turbidity difference reaches
91.6%.
The cutinase hydrolysed residual plain boiled water in 12 source Humicola Insolens of table
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not intended to limit the invention, any to be familiar with this skill
The people of art can do various change and modification, therefore protection model of the invention without departing from the spirit and scope of the present invention
Enclosing subject to the definition of the claims.
Claims (1)
1. a kind of method of resinous matter in degradation mthod of white water from paper making, which is characterized in that the method be by cutinase with 0.5~
The additive amount of 8000U/g substrate is added into the solution of resinous substance, and control processing 30~240min of time, pH are 7~9
Carry out enzymolysis processing；The concentration of the resinous matter is 4~400g/L；The solution of the resinous substance is paper-making process
The remaining plain boiled water of middle plain boiled water secondary cycle contains at least one following substances: olein, polyvinyl acetate, rosin
Acid esters, lignin aliphatic ester, resin acid, specifically there are several types of methods:
(a) plain boiled water pH to 8.5 ± 0.2 is adjusted, the cutinase in the source Thermobifida fusca, 0.5~1U/ of enzyme concentration is added
G is handled 3 hours, the water quality of plain boiled water: 50~200mg/L of COD, pH 7.0,200~1000NTU of turbidity at 16~25 DEG C；
(b) alternatively, adjusting plain boiled water pH to 8.0 ± 0.2, the cutinase in the addition source Fusarium solani, enzyme concentration 2.67~
5.33U/g is handled 4 hours, the water quality of plain boiled water at 16~25 DEG C: 50~400mg/L of COD, pH 7.0, turbidity 200~
1000NTU；
(c) alternatively, adjusting plain boiled water pH to 8.0 ± 0.2, the cutinase in the source Humicola Insolens, enzyme concentration 2.5 is added
~5U/g is handled 4 hours, the water quality of plain boiled water at 16~25 DEG C: 50~400mg/L of COD, pH 7.0, turbidity 200~
1000NTU。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611203776.9A CN106480771B (en) | 2016-12-23 | 2016-12-23 | A method of utilizing cutin enzymatic treatment mthod of white water from paper making |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611203776.9A CN106480771B (en) | 2016-12-23 | 2016-12-23 | A method of utilizing cutin enzymatic treatment mthod of white water from paper making |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106480771A CN106480771A (en) | 2017-03-08 |
| CN106480771B true CN106480771B (en) | 2018-12-04 |
Family
ID=58285903
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611203776.9A Active CN106480771B (en) | 2016-12-23 | 2016-12-23 | A method of utilizing cutin enzymatic treatment mthod of white water from paper making |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106480771B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108251319A (en) * | 2018-02-12 | 2018-07-06 | 江南大学 | A kind of fermentation of cutinase and cotton fabric enzyme refinery practice |
| CN108753671A (en) * | 2018-06-05 | 2018-11-06 | 江南大学 | A kind of the recombination bacillus coli engineering bacteria and its zymotechnique of high yield cutinase |
| CN109929821A (en) * | 2018-12-27 | 2019-06-25 | 江南大学 | A kind of cutin enzyme mutant and its application in plain boiled water processing |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR0112177A (en) * | 2000-06-16 | 2003-09-02 | Buckman Labor Inc | Methods for controlling organic contaminants in fibers containing organic contaminants and for making paper or cardboard products |
| WO2007095575A1 (en) * | 2006-02-14 | 2007-08-23 | Novozymes North America, Inc | Chemical pulp treatment compositions and methods |
| AU2008254214B2 (en) * | 2007-05-16 | 2012-03-08 | Buckman Laboratories International, Inc. | Methods to control organic contaminants in fibers |
| US20130180677A1 (en) * | 2012-01-12 | 2013-07-18 | Buckman Laboratories International, Inc. | Methods To Control Organic Contaminants In Fibers |
| CN103061184A (en) * | 2012-12-26 | 2013-04-24 | 泰兴市一鸣生物制品有限公司 | Method of controlling secondary gummy substances by using lipase |
| CN103074795B (en) * | 2012-12-26 | 2015-04-29 | 泰兴市一鸣生物制品有限公司 | Method for controlling secondary stickies through lipase |
| US20150053358A1 (en) * | 2013-08-20 | 2015-02-26 | Buckman Laboratories International, Inc. | Methods To Control Organic Contaminants In Fibers Using Zeolites |
| CN103757960B (en) * | 2013-12-31 | 2017-01-25 | 江南大学 | Method for applying cutinase to papermaking industry |
| CN105040505B (en) * | 2015-06-30 | 2017-08-25 | 华南理工大学 | A kind of method that aspergillus oryzae whole-cell catalyst handles mthod of white water from paper making resin |
| CN105386352B (en) * | 2015-11-17 | 2018-05-04 | 江南大学 | It is a kind of to utilize cutinase and the method for the common deinking of chemical reagent |
-
2016
- 2016-12-23 CN CN201611203776.9A patent/CN106480771B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106480771A (en) | 2017-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Das et al. | Production of cellulolytic enzymes by Aspergillus fumigatus ABK9 in wheat bran-rice straw mixed substrate and use of cocktail enzymes for deinking of waste office paper pulp | |
| CN106480771B (en) | A method of utilizing cutin enzymatic treatment mthod of white water from paper making | |
| Alam et al. | Production and characterization of thermostable xylanases by Thermomyces lanuginosus and Thermoascus aurantiacus grown on lignocelluloses | |
| Sridevi et al. | Biobleaching of paper pulp with xylanase produced by Trichoderma asperellum | |
| Ninawe et al. | Bleaching of wheat straw-rich soda pulp with xylanase from a thermoalkalophilic Streptomyces cyaneus SN32 | |
| LU500846B1 (en) | Method for pulping wheat straw by using xylanase and pectinase | |
| Heck et al. | Statistical optimization of thermo-tolerant xylanase activity from Amazon isolated Bacillus circulans on solid-state cultivation | |
| CN105970733A (en) | Method for improving paper strengthening effect of bacterial cellulose-based paper strengthening agent | |
| CN104213450A (en) | Deinking treatment method of mixed office waste paper | |
| Zhang et al. | Control of sticky deposits in wastepaper recycling with thermophilic esterase | |
| CN110512458B (en) | Treatment process for removing stickies in paper pulp | |
| Angural et al. | Bleach enhancement of mixed wood pulp by mixture of thermo-alkali-stable xylanase and mannanase derived through co-culturing of Alkalophilic Bacillus sp. NG-27 and Bacillus nealsonii PN-11 | |
| Singh et al. | Characterization of lignin peroxidase from Paecilomyces species for decolorisation of pulp and paper mill effluent | |
| Ghoshal et al. | Xylanase production by Penicillium citrinum in laboratory-scale stirred tank reactor | |
| CN104928270B (en) | The recombinant basic zytase and its construction method of one group of ethylenediamine tetra-acetic acid of resistance to chelating agent | |
| LH et al. | Improvement of laccase production by Pleurotus ostreatus by means of agroindustrial waste and fermentation kinetics | |
| CN103757960B (en) | Method for applying cutinase to papermaking industry | |
| CN108585343A (en) | The recycle device and processing method of papermaking enriched water | |
| Sondhi et al. | Enzymatic Approach for Bioremediation of Effluent from Pulp and Paper Industry by Thermo Alkali Stable Laccase from Bacillus Tequilensis SN4. | |
| Ramadhani et al. | The potency of microbial flocculant produced by B. licheniformis using molasses as the carbon source and its application in food industry wastewater treatment | |
| CN104213448A (en) | Laccase/glutamic acid-based biological treatment method for inhibiting strength degradation of regenerated fibers | |
| CN105386352A (en) | Common deinking method employing cutinase and chemical reagents | |
| Vlaev et al. | Cellulase production by Trichoderma sp. grown on corn fibre substrate | |
| Lipin et al. | Delignification of Kraft Pulp by Lipase | |
| Ćilerdžić et al. | From pomiculture waste to biotechnological raw material: efficient transformation using ligninosomes and cellulosomes from Pleurotus spp. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |