CN105040505A - Method for processing papermaking white water resin through aspergillus oryzae whole-cell catalyst - Google Patents
Method for processing papermaking white water resin through aspergillus oryzae whole-cell catalyst Download PDFInfo
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- CN105040505A CN105040505A CN201510382996.1A CN201510382996A CN105040505A CN 105040505 A CN105040505 A CN 105040505A CN 201510382996 A CN201510382996 A CN 201510382996A CN 105040505 A CN105040505 A CN 105040505A
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- aspergillus oryzae
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21F—PAPER-MAKING MACHINES; METHODS OF PRODUCING PAPER THEREON
- D21F1/00—Wet end of machines for making continuous webs of paper
- D21F1/66—Pulp catching, de-watering, or recovering; Re-use of pulp-water
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Abstract
The invention discloses a method for processing papermaking white water resin through an aspergillus oryzae whole-cell catalyst. According to the method, firstly, different culture mediums are adopted, aspergillus oryzae is screened, the aspergillus oryzae whole-cell catalyst with the highest enzyme activity is selected for an experiment, and aspergillus oryzae culture conditions are optimized; the aspergillus oryzae whole-cell catalyst obtained through culture under the optimal condition is used for processing a papermaking white water model complex, a product obtained by catalyzing and degrading papermaking white water trough the aspergillus oryzae whole-cell catalyst is analyzed, and accordingly processing conditions of the aspergillus oryzae whole-cell catalyst on the papermaking white water are determined. According to the method, dissolved and colloid substances (DCS) in the papermaking white water are catalyzed and decomposed through the aspergillus oryzae by means of the whole-cell catalyst method, the cationic demand (CD) of the white water is reduced, adverse effects brought by closed circulating of the papermaking white water is alleviated, and the problems of nonrepeatability and high processing cost of common free enzyme in the papermaking white water processing process are solved. The method has the advantages of being low in cost, environmentally friendly and the like.
Description
Technical field
The invention belongs to wet end chemistry technical field, be specifically related to the method for Aspergillus oryzae whole-cell catalyst in process mthod of white water from paper making.
Background technology
The reuse of mthod of white water from paper making not only can save the consumption of clear water, reduces the loss of fiber and chemicals, saving resource and reducing costs, and reduces environmental pollution and also has great importance.But along with the raising of white water system degree, the harmful substance in plain boiled water also can accumulate gradually.In these harmful substances, wet end chemistry is had the greatest impact, the most unmanageable be dissolved substance in plain boiled water and colloidal substance (DCS).DCS mono-aspect derives from the chemicals of various organic and inorganic additive and the application added in the natural materials such as solubility wood extractive (as Small molecular lignin, starch, carbohydrate), resin and water and production process.In plain boiled water DCS, resinous matter is that some are dissolved in the hydrophobic substance of neutral organic solvent, and this part material can be deposited on equipment surface in a variety of forms in paper-making process, causes and shuts down and degradation problem under papery.
Conventional processing method for problems mainly utilizes cationic polyelectrolyte to neutralize, and this process not only needs to consume a large amount of chemicals, and processing cost is high, can increase the follow-up water treatment difficulty of papermaking simultaneously.Enzyme preparation due to have efficient, reaction condition is gentle, advantages of environment protection, be applied to pulping and papermaking processes solving bio-pulping, pitch trouble, improve fibre property, the problem such as bio-bleaching and wastewater treatment.
Resolvase enzyme in the White Water Treatment process retention time alive adopted due to current business-like enzyme preparation is shorter, and do not possess repeatability, in addition, processing cost is also high, and these all limit enzyme in papermaking engineering large-scale promotion and application.If directly process mthod of white water from paper making with whole-cell catalyst, stablizing of catalyst can be improved, catalyst recycling, greatly will reduce the processing cost of mthod of white water from paper making, have more wide application prospect.Due to the difference of culture medium, considerable influence can be produced to the activity of Aspergillus oryzae and purposes.The Aspergillus oryzae that the present invention adopts Tween 80 to induce processes mthod of white water from paper making as whole-cell catalyst, and adopts the optimal treatment condition of mthod of white water from paper making resin mould thing to Aspergillus oryzae whole-cell catalyst to study.
Summary of the invention
In order to overcome the shortcoming and defect part of above-mentioned prior art, the invention provides a kind of environmental friendliness, production cost is low, the method for Aspergillus oryzae whole-cell catalyst process mthod of white water from paper making that course of reaction is simply controlled.
Object of the present invention is achieved through the following technical solutions.
Adopt a method for Aspergillus oryzae whole-cell catalyst process mthod of white water from paper making, comprise the following steps:
(1) screening of Aspergillus oryzae, adopts Tween 80 induction Aspergillus oryzae 35;
(2) lipase of Aspergillus oryzae 35 fermenting and producing step (1) obtained is for the treatment of mthod of white water from paper making resin mould thing.
Further, step (1) described employing Tween 80 induction Aspergillus oryzae 35 specifically: to contain 0.1% ~ 1.5% (NH
4)
2sO
4, 0.1% ~ 1.0% Tween 80,0.01% ~ 0.2% yeast extract (Yeastextract), 0.01% ~ 0.1%K
2hPO
4, 0.01 ~ 0.05%MgSO
47H
2the culture medium of O, cultivation and fermentation Aspergillus oryzae 35; The Calculation Basis of described percentage is the gross mass to account for culture medium.
Further, described lipase active reaches 100 ~ 300U/g, can be applied to the processing requirements of mthod of white water from paper making completely.
Further, described plain boiled water resin mould thing is olein or lignin fatty acid ester, or resin acid, glyceric acid fat or unsaponifiable matter.
Further, step (2) is specifically: it is in the phosphate buffer of 7 ~ 8 that mthod of white water from paper making resin mould thing is added PH, and then add the lipase of Aspergillus oryzae 35 fermenting and producing that step (1) obtains, be lower reaction 20 ~ 120min at 20 ~ 70 DEG C, in reaction system, concentration of substrate is the volume ratio of 30 ~ 50%(olein and phosphate buffer).
The present invention can effectively degrade the object module things such as lipid and some unsaponifiable matter such as olein, aliphatic acid, resin acid, glyceric acid fat, is corresponding acid by its catalytic decomposition.Utilize NaOH titer to carry out the oleic acid of titration generation, judge the amount generating acid, thus infer the decomposition situation of Aspergillus oryzae to object module thing.
Principle of the present invention is: the chemical composition of resin mainly contains stationery and some unsaponifiable matter etc. such as aliphatic acid, resin acid, glyceric acid fat.Lipid material in resin can be hydrolyzed into the resin acid and alcohols etc. of low stickiness by lipase, alleviates or eliminate the problem on obstacle of resin.
Compared with prior art, the present invention has the following advantages and beneficial effect:
(1) method of the present invention adopts whole-cell catalyst that is efficient, high stability to carry out catalytic decomposition olein, overcomes the easy inactivation of enzyme in prior art, the shortcoming that reaction efficiency is low;
(2) reaction condition is gentle, environmental friendliness;
(3) the whole-cell catalyst Aspergillus oryzae needed for reaction is produced simple, cheaply, greatly reduces production cost, improves production efficiency.
(4) output of Aspergillus oryzae is more considerable compared to other bacterial fungus class cells, and this is also that further industrial applicability may improve.
Accompanying drawing explanation
Fig. 1 is the result of reaction temperature to Aspergillus oryzae catalytic decomposition olein.
Fig. 2 is the result of PH to Aspergillus oryzae catalytic decomposition olein.
Fig. 3 is the result of concentration of substrate to Aspergillus oryzae catalytic decomposition olein.
Detailed description of the invention
For better understanding the present invention, below in conjunction with embodiment the present invention done and describe in detail further, but the scope of protection of present invention being not limited to the scope that embodiment represents.
The screening of Aspergillus oryzae, due to the difference of culture medium, considerable influence can be produced to the activity of Aspergillus oryzae and purposes, glucose induction and Tween 80 is adopted to induce two kinds of different culture medias, and two kinds of Aspergillus oryzaes 3.4862, Aspergillus oryzae 3.5232(is purchased from Institute of Microorganism, Academia Sinica), Dual culture compares the lipase active of 4 kinds of different Aspergillus oryzaes, selects lipase active the highest relative Aspergillus oryzae to test.Under Tween 80 inductive condition, Aspergillus oryzae 35 lipase active of gained is the highest, can be applied to the resin deposit processing requirements of mthod of white water from paper making.So the present invention adopt Tween 80 to induce Aspergillus oryzae 35 as whole-cell catalyst to process mthod of white water from paper making pitch deposition object model.
Embodiment 1
A method for Aspergillus oryzae process mthod of white water from paper making resin, comprises the following steps:
(1) primary election of Aspergillus oryzae.Be 0.5% (NH by Tween 80 at condition of culture
4)
2sO
4, 0.5% Tween 80,0.1% yeast extract (Yeastextract), 0.1%K
2hPO
4, 0.02%MgSO
47H
2o seed culture medium, the Aspergillus oryzae of induction is as whole-cell catalyst process mthod of white water from paper making pitch deposition object model (triglyceride, lower same).
(2) lipase of Aspergillus oryzae 35 fermenting and producing step (1) obtained is for the treatment of mthod of white water from paper making resin mould thing.Taking 15mL olein, to join PH be in the 15mL phosphate buffer of 7.5, the lipase of 0.3g Aspergillus oryzae fermenting and producing is added after mixing, accurately react 60min in 45 DEG C of water-baths after, get 6mL sample and join cessation reaction in the ethanol of 15mL95%, then utilize 0.005mol/L NaOH titer on potentiometric titrimeter, to be titrated to pH10.3 for terminal.Volume according to the NaOH titer of titration consumption judges the decomposition situation of Aspergillus oryzae to olein.The Aspergillus oryzae catalytic decomposition olein of the present embodiment, the degradation rate of olein is in table 1.
Embodiment 2
A method for Aspergillus oryzae process mthod of white water from paper making resin, comprises the following steps:
(1) primary election of Aspergillus oryzae.Be 0.5% (NH by Tween 80 at condition of culture
4)
2sO
4, 0.5% Tween 80,0.1% yeast extract (Yeastextract), 0.1%K
2hPO
4, 0.02%MgSO
47H
2o seed culture medium, the Aspergillus oryzae of induction is as whole-cell catalyst process mthod of white water from paper making pitch deposition object model.
(2) lipase of Aspergillus oryzae 35 fermenting and producing step (1) obtained is for the treatment of mthod of white water from paper making resin mould thing.Taking 15mL olein, to join PH be in the 15mL phosphate buffer of 9.0, the lipase of 0.3g Aspergillus oryzae fermenting and producing is added after mixing, accurately react 30min in 45 DEG C of water-baths after, get 6mL sample and join cessation reaction in the ethanol of 15mL95%, then utilize 0.005mol/L NaOH titer on potentiometric titrimeter, to be titrated to pH10.3 for terminal.Volume according to the NaOH titer of titration consumption judges the decomposition situation of Aspergillus oryzae to olein.The Aspergillus oryzae catalytic decomposition olein of the present embodiment, the degradation rate of olein is in table 1.
Embodiment 3
A method for Aspergillus oryzae process mthod of white water from paper making resin, comprises the following steps:
(1) primary election of Aspergillus oryzae.Be 0.5% (NH by Tween 80 at condition of culture
4)
2sO
4, 0.5% Tween 80,0.1% yeast extract (Yeastextract), 0.1%K
2hPO
4, 0.02%MgSO
47H
2o seed culture medium, the Aspergillus oryzae of induction is as whole-cell catalyst process mthod of white water from paper making pitch deposition object model.
(2) lipase of Aspergillus oryzae 35 fermenting and producing step (1) obtained is for the treatment of mthod of white water from paper making resin mould thing.Taking 5mL olein, to join PH be in the 25mL phosphate buffer of 7.5, the lipase of 0.3g Aspergillus oryzae fermenting and producing is added after mixing, accurately react 30min in 45 DEG C of water-baths after, get 6mL sample and join cessation reaction in the ethanol of 15mL95%, right, it is terminal that how many buildings potentiometric titrimeter are titrated to pH10.3.Volume according to the NaOH titer of titration consumption judges the decomposition situation of Aspergillus oryzae to olein.The Aspergillus oryzae catalytic decomposition olein of the present embodiment, the degradation rate of olein is in table 1.
Embodiment 4
A method for Aspergillus oryzae process mthod of white water from paper making resin, comprises the following steps:
(1) primary election of Aspergillus oryzae.Be 0.5% (NH by Tween 80 at condition of culture
4)
2sO
4, 0.5% Tween 80,0.1% yeast extract (Yeastextract), 0.1%K
2hPO
4, 0.02%MgSO
47H
2o seed culture medium, the Aspergillus oryzae of induction is as whole-cell catalyst process mthod of white water from paper making pitch deposition object model.
(2) lipase of Aspergillus oryzae 35 fermenting and producing step (1) obtained is for the treatment of mthod of white water from paper making resin mould thing.Taking 25mL olein, to join PH be in the 5mL phosphate buffer of 7.5, the lipase of 0.3g Aspergillus oryzae fermenting and producing is added after mixing, accurately react 10min in 45 DEG C of water-baths after, get 6mL sample and join cessation reaction in the ethanol of 15mL95%, then utilize 0.005mol/L NaOH titer on potentiometric titrimeter, to be titrated to pH10.3 for terminal.Volume according to the NaOH titer of titration consumption judges the decomposition situation of Aspergillus oryzae to olein.The Aspergillus oryzae catalytic decomposition olein of the present embodiment, the degradation rate of olein is in table 1.
Embodiment 5
A method for Aspergillus oryzae process mthod of white water from paper making resin, comprises the following steps:
(1) primary election of Aspergillus oryzae.Be 0.5% (NH by Tween 80 at condition of culture
4)
2sO
4, 0.5% Tween 80,0.1% yeast extract (Yeastextract), 0.1%K
2hPO
4, 0.02%MgSO
47H
2o seed culture medium, the Aspergillus oryzae of induction is as whole-cell catalyst process mthod of white water from paper making pitch deposition object model.
(2) lipase of Aspergillus oryzae 35 fermenting and producing step (1) obtained is for the treatment of mthod of white water from paper making resin mould thing.Taking 15mL olein, to join PH be in the 15mL phosphate buffer of 8.5, the lipase of 0.3g Aspergillus oryzae fermenting and producing is added after mixing, accurately react 120min in 45 DEG C of water-baths after, get 6mL sample and join cessation reaction in the ethanol of 15mL95%, then utilize 0.005mol/L NaOH titer on potentiometric titrimeter, to be titrated to pH10.3 for terminal.Volume according to the NaOH titer of titration consumption judges the decomposition situation of Aspergillus oryzae to olein.The Aspergillus oryzae catalytic decomposition olein of the present embodiment, the degradation rate of olein is in table 1.
Embodiment 6
A method for Aspergillus oryzae process mthod of white water from paper making resin, comprises the following steps:
(1) primary election of Aspergillus oryzae.Be 0.5% (NH by Tween 80 at condition of culture
4)
2sO
4, 0.5% Tween 80,0.1% yeast extract (Yeastextract), 0.1%K
2hPO
4, 0.02%MgSO
47H
2o seed culture medium, the Aspergillus oryzae of induction is as whole-cell catalyst process mthod of white water from paper making pitch deposition object model.
(2) lipase of Aspergillus oryzae 35 fermenting and producing step (1) obtained is for the treatment of mthod of white water from paper making resin mould thing.Taking 15mL olein, to join PH be in the 15mL phosphate buffer of 8.5, the lipase of 0.3g Aspergillus oryzae fermenting and producing is added after mixing, accurately react 60min in 65 DEG C of water-baths after, get 4mL sample and join cessation reaction in the ethanol of 15mL95%, then utilize 0.005mol/L NaOH titer on potentiometric titrimeter, to be titrated to pH10.3 for terminal.Volume according to the NaOH titer of titration consumption judges the decomposition situation of Aspergillus oryzae to olein.The Aspergillus oryzae catalytic decomposition olein of the present embodiment, the degradation rate of olein is in table 1.
Embodiment 7
A method for Aspergillus oryzae process mthod of white water from paper making resin, comprises the following steps:
(1) primary election of Aspergillus oryzae.Be 0.5% (NH by Tween 80 at condition of culture
4)
2sO
4, 0.5% Tween 80,0.1% yeast extract (Yeastextract), 0.1%K
2hPO
4, 0.02%MgSO
47H
2o seed culture medium, the Aspergillus oryzae of induction is as whole-cell catalyst process mthod of white water from paper making pitch deposition object model.
(2) lipase of Aspergillus oryzae 35 fermenting and producing step (1) obtained is for the treatment of mthod of white water from paper making resin mould thing.Taking 15mL olein, to join PH be in the 15mL phosphate buffer of 8.5, the lipase of 0.3g Aspergillus oryzae fermenting and producing is added after mixing, accurately react 15min in 55 DEG C of water-baths after, get 4mL sample and join cessation reaction in the ethanol of 15mL95%, then utilize 0.005mol/L NaOH titer on potentiometric titrimeter, to be titrated to pH10.3 for terminal.Volume according to the NaOH titer of titration consumption judges the decomposition situation of Aspergillus oryzae to olein.The Aspergillus oryzae catalytic decomposition olein of the present embodiment, the degradation rate of olein is in table 1.
The result of table 1 embodiment 1-7 Aspergillus oryzae catalytic decomposition olein
| Sample | Concentration of substrate | Temperature | PH | Reaction time | Degradation rate |
| Embodiment 1 | 50% | 45 | 7.5 | 60min | 94.8% |
| Embodiment 2 | 50% | 45 | 9.0 | 30min | 43.5% |
| Embodiment 3 | 16.7% | 45 | 7.5 | 30min | 50.9% |
| Embodiment 4 | 83.3% | 45 | 7.5 | 10min | 37.9% |
| Embodiment 5 | 50% | 45 | 8.5 | 120min | 73.8% |
| Embodiment 6 | 50% | 65 | 8.5 | 60min | 38.2% |
| Embodiment 7 | 50% | 55 | 8.5 | 15min | 45.7% |
As can be seen from Table 1, the present invention utilizes Aspergillus oryzae process mthod of white water from paper making resin mould thing, and the degradation rate of olein is very high, infers that plain boiled water resin mould thing is decomposed thus.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; modification, the change made under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be all included within protection scope of the present invention.
Claims (5)
1. adopt a method for Aspergillus oryzae whole-cell catalyst process mthod of white water from paper making, it is characterized in that, comprise the following steps:
(1) screening of Aspergillus oryzae, adopts Tween 80 induction Aspergillus oryzae 35;
(2) lipase of Aspergillus oryzae 35 fermenting and producing step (1) obtained is for the treatment of mthod of white water from paper making resin mould thing.
2. a kind of method adopting Aspergillus oryzae whole-cell catalyst process mthod of white water from paper making according to claim 1, is characterized in that, the described employing Tween 80 of step (1) induces Aspergillus oryzae 35 specifically: to contain 0.1% ~ 1.5% (NH
4)
2sO
4, 0.1% ~ 1.0% Tween 80,0.01% ~ 0.2% yeast extract (Yeastextract), 0.01% ~ 0.1%K
2hPO
4, 0.01 ~ 0.05%MgSO
47H
2the culture medium of O, cultivation and fermentation Aspergillus oryzae 35; The Calculation Basis of described percentage is the gross mass to account for culture medium.
3. the method for Aspergillus oryzae process mthod of white water from paper making resin according to claim 1, it is characterized in that, described lipase active reaches 100 ~ 300U/g, can be applied to the processing requirements of mthod of white water from paper making completely.
4. the method for Aspergillus oryzae process mthod of white water from paper making resin according to claim 1, is characterized in that, described plain boiled water resin mould thing is olein, lignin fatty acid ester, resin acid, glyceric acid fat or unsaponifiable matter.
5. the method for Aspergillus oryzae process mthod of white water from paper making resin according to claim 1, it is characterized in that, step (2) is specifically: it is in the phosphate buffer of 7 ~ 8 that mthod of white water from paper making resin mould thing is added PH, and then add the lipase of Aspergillus oryzae 35 fermenting and producing that step (1) obtains, be lower reaction 20 ~ 120min at 20 ~ 70 DEG C, in reaction system, concentration of substrate is 30 ~ 50%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106480771A (en) * | 2016-12-23 | 2017-03-08 | 江南大学 | A kind of method of utilization cutin ferment treatment mthod of white water from paper making |
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| US4221634A (en) * | 1979-02-21 | 1980-09-09 | Federal Paper Board Company, Inc. | Method of treating papermaking white water |
| CN101418287A (en) * | 2008-11-28 | 2009-04-29 | 天津科技大学 | Aspergillus niger liquid state fermentation pectic enzyme and control to colloidal matter in white water and paper stuff |
| CN103061184A (en) * | 2012-12-26 | 2013-04-24 | 泰兴市一鸣生物制品有限公司 | Method of controlling secondary gummy substances by using lipase |
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| CN106480771A (en) * | 2016-12-23 | 2017-03-08 | 江南大学 | A kind of method of utilization cutin ferment treatment mthod of white water from paper making |
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