CN104561189A - Method for improving hydrolysis efficiency of corn straw and obtaining xylose and glucose simultaneously - Google Patents
Method for improving hydrolysis efficiency of corn straw and obtaining xylose and glucose simultaneously Download PDFInfo
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Abstract
The invention discloses a method for improving the hydrolysis efficiency of corn straw and obtaining xylose and glucose simultaneously, and relates to a method for improving the corn straw hydrolysis efficiency and obtaining xylose and glucose simultaneously. The method aims at reducing the probability of precipitation side reaction of extract during hydrothermal pretreatment, hydrolyzing pretreated hemicellulose by adding xylanase and Beta-xylosidase, and improving the hydrolysis efficiency of corn straw subjected to hydrothermal pretreatment and obtaining xylose. The method comprises the following steps: crushing the corn straw, mixing the crushed corn straw with 5%-ethyl alcohol, shaking, separating and collecting solid-phase materials, drying the solid-phase materials, adding the solid-phase materials to a reaction kettle, heating to 180 to 200 DEG C and keeping the temperature for 20 min, and adding xylanase, Beta-xylosidase, cellulose and Beta-glucosaccharase for enzymolysis. According to the method, the extract is removed before hydrothermal pretreatment, the probability of precipitation side reaction of the extract during pretreatment is furthest reduced, the hindering effect on the cellulose by precipitate is reduced, and the hydrolysis efficiency is improved.
Description
Technical field
The present invention relates to technical field of biomass resource utilization, specifically a kind of pre-treatment of maize straw and the method for enzymolysis.
Background technology
Utilize the reproducible lignocellulose based biomass such as such as maize straw to produce fuel and namely can alleviate environmental pollution, also can alleviate the dependence to Nonrenewable energy resources such as oil.The main component of lignocellulose based biomass is Mierocrystalline cellulose, hemicellulose and xylogen.At present, mainly utilize cellulase hydrolysis Mierocrystalline cellulose to produce glucose to the application approach of lignocellulose based biomass, recycling glucose fermentation produces ethanol, the fuel such as butanols.Due to hemicellulose and xylogen and Mierocrystalline cellulose structurally mutually wrap up winding cause Mierocrystalline cellulose and cellulase contact area less, the efficiency of the direct enzymic hydrolysis of Mierocrystalline cellulose is lower.In order to improve the enzymic hydrolysis efficiency of lignocellulose thus obtain higher fuel output, raw material had to pass through suitable pre-treatment before carrying out enzymic hydrolysis, reduction hemicellulose and xylogen are wound around cellulosic parcel, increased fiber element enzyme and cellulosic contact area.The pretreatment technology that oneself has comprises physical method, chemical process, physico-chemical process, biological method etc.Wherein, hydrothermal pretreatment is easy to amplify and the advantage such as environmentally friendly with it, and enjoys favor.This technique is carried out at 120-260 DEG C usually, only relies on the H that high-pressure water heating self produces
+catalyzed reaction, cause hemicellulose and cellulosic syndeton to be destroyed, part hemicellulose is hydrolyzed, and improves cellulase and cellulosic contact area.Extract is a series of being extensively present in the middle of plant stem-leaf, with lignocellulose (Mierocrystalline cellulose, hemicellulose, xylogen) be connected without covalent linkage, can apply the general name of the compound that opposed polarity or non-polar solvent extract from biomass, the extractives content scope in herbaceous plant stem is generally between 10-25%.The composition of extract mainly includes machine acid, nitrogenous compound, chlorophyll, wax, carbohydrate, phenols, the materials such as alkaloid.Thammasouk, Tamaki and Zimbardi etc. all report that some compositions in extract can form precipitation in acid condition.These precipitations of pointing out Ballesteros etc. can hinder cellulase to contact with cellulosic, thus cut down enzymolysis efficiency.In addition, ligno-cellulosic materials is that whole technique (pre-treatment, enzymolysis, fermentation and distillation) cost is higher as the bottleneck of raw material production fuel ethanol industry.
Summary of the invention
The object of the invention is the generation precipitating side reaction in order to reduce extract in hydrothermal pretreatment process, and by adding zytase and xylobiase is hydrolyzed pretreated hemicellulose, after improving hydrothermal pretreatment maize straw enzymolysis efficiency and obtain wood sugar.
Of the present inventionly a kind ofly improve maize straw enzymolysis efficiency and obtain the method for xylose and glucose simultaneously, it carries out according to following steps:
One, cut off by maize straw, being then crushed to granularity is 0.250 ~ 0.425mm;
Two, be then that 5% ethanolic soln mixes with the ratio of solid-to-liquid ratio 1:15 by the maize straw after pulverizing in step one with mass percentage, under room temperature, rotating speed are the condition of 150r/min, jolting 24h, then through solid-liquid separation, collect solid formation, solid formation is dried 12h under 35 DEG C of conditions;
Three, after the solid formation after oven dry in step 2 being mixed according to the ratio that solid-to-liquid ratio is 1g:8 ~ 12mL with water, be transferred in hydrothermal reaction kettle, reactor is heated to 180 ~ 200 DEG C, after maintaining 20min, reactor is immersed in mixture of ice and water and terminates hydro-thermal reaction;
Four, pH value to 4.5 ~ 5.0 of the reaction solution of set-up procedure three, then zytase and xylobiase is added, temperature be 50 DEG C, under rotating speed is the condition of 150rpm, enzymolysis 48h, add cellulase and beta-glucosidase again, temperature be 50 DEG C, under rotating speed is the condition of 150rpm, enzymolysis 24h, obtains glucose and xylose; Wherein, zytase and xylobiase addition are 2400U/g Mierocrystalline cellulose, and the addition of cellulase is 15FPU/g Mierocrystalline cellulose, and beta-glucosidase addition is 15CBU/g Mierocrystalline cellulose.
Design of the present invention:
(1) in hydrothermal pretreatment process, only use water as medium, under high temperature, water power plays the effect hydrolyzed hemicellulose of acid from the oxonium ion produced, hydrolysis of hemicellulose generates the acids such as acetic acid, uronic acid and phenolic acid, cause the further degraded of biomass, this process is referred to as the hydrolysis certainly of biomass.Maize straw is owing to containing higher extractives content, in preprocessing process, precipitation can be formed be attached to maize straw surface owing to being in sour environment extract, in follow-up enzymic hydrolysis process, reduce cellulase and cellulosic contact area, thus cut down enzymatic hydrolyzation.If before pre-processing, the extract in maize straw is removed as far as possible, then can farthest reduce in preprocessing process the generation precipitating side reaction.
(2) although partial solution is wound around except hemicellulose and Mierocrystalline cellulose parcel structurally and has part hemicellulose to be hydrolyzed in hydrothermal pretreatment process, increase cellulase and cellulosic contact area, but still have part hemicellulose not to be hydrolyzed and remain in solids, cellulase is hindered to contact with cellulosic, if now zytase can be added and xylobiase is hydrolyzed this part hemicellulose, then can improve cellulosic enzymolysis efficiency further.In addition, add zytase and xylobiase and not only decrease steric restriction between cellulase and Mierocrystalline cellulose, can also the xylan in unhydrolyzed solids in hemicellulose and pretreatment fluid be wood sugar, while acquisition higher fiber element enzymolysis efficiency, can wood sugar be obtained again.
The present invention has following characteristics and advantage:
1. the present invention removes extract before hydrothermal pretreatment, reduces the probability that side reaction occurs to precipitate extract in preprocessing process to greatest extent, reduces the inhibition of sedimentable matter to cellulase, and then improve enzymolysis efficiency.
2. in preprocessing process, because extract is attached to maize straw surface, oxonium ion and maize straw contact area are reduced, causes hydrothermal pretreatment effect to reduce, after removing extract, hydrothermal pretreatment effect can be improved.
3. in pretreated solidliquid mixture, add zytase and xylobiase to be hydrolyzed after pre-treatment still residual part hemicellulose in solids, further increase cellulase and cellulosic contact area, improve enzymolysis efficiency.
4. in pretreated solidliquid mixture, add zytase and xylobiase can xylan be wood sugar in hemicellulose in unhydrolyzed solids and pretreatment fluid, while improve enzymolysis efficiency (obtaining comparatively glucosan), obtain wood sugar, improve the utilization ratio of maize straw.
The present invention adopts total composition to apply and the theory of biorefining: namely while guarantee Mierocrystalline cellulose acquisition high enzyme solution rate the value product that also obtains from hemicellulose and xylogen as much as possible to reduce costs.The theory of phenomenon in conjunction with biorefining of precipitation is formed in acid condition for extract, the present invention applies 5% ethanol-water solution and removes extract, to reduce the generation that extract precipitates side reaction in hydrothermal pretreatment process, increased fiber element enzyme and cellulosic contact area, lowers its impact on follow-up enzymolysis, in addition, solid-liquid separation is not carried out after pre-treatment, zytase and xylobiase is added directly in pretreated mixture, by still retaining hemicellulose in solids after hydrolysis pre-treatment with increased fiber element enzyme and cellulosic contact area, improve cellulase hydrolysis rate, the part hemicellulose in solid phase and the acquisition of the xylan in pretreatment fluid wood sugar is still present in after the zytase simultaneously added and xylobiase can also be hydrolyzed pre-treatment, wood sugar is obtained while raising cellulase hydrolysis efficiency (obtaining more glucosan), improve the utilization ratio of maize straw.
Accompanying drawing explanation
Fig. 1 be through embodiment 1 remove extract carry out hydrothermal pretreatment after maize straw scanning electron microscopic picture;
Fig. 2 is the maize straw scanning electron microscopic picture of comparative example 1 after hydrothermal treatment consists;
Fig. 3 be through embodiment 2 remove extract carry out hydrothermal pretreatment after hydrothermal treatment consists after maize straw scanning electron microscopic picture;
Fig. 4 is the maize straw scanning electron microscopic picture of comparative example 2 after hydrothermal treatment consists;
Fig. 5 be through embodiment 3 remove extract carry out hydrothermal pretreatment after hydrothermal treatment consists after maize straw scanning electron microscopic picture;
Fig. 6 is the maize straw scanning electron microscopic picture of comparative example 3 after hydrothermal treatment consists.
Embodiment
Embodiment one: a kind of of present embodiment improves the method that maize straw enzymolysis efficiency also obtains xylose and glucose simultaneously, and it carries out according to following steps:
One, cut off by maize straw, being then crushed to granularity is 0.250 ~ 0.425mm;
Two, be then that 5% ethanolic soln mixes with the ratio of solid-to-liquid ratio 1:15 by the maize straw after pulverizing in step one with mass percentage, under room temperature, rotating speed are the condition of 150r/min, jolting 24h, then through solid-liquid separation, collect solid formation, solid formation is dried 12h under 35 DEG C of conditions;
Three, after the solid formation after oven dry in step 2 being mixed according to the ratio that solid-to-liquid ratio is 1g:8 ~ 12mL with water, be transferred in hydrothermal reaction kettle, reactor is heated to 180 ~ 200 DEG C, after maintaining 20min, reactor is immersed in mixture of ice and water and terminates hydro-thermal reaction;
Four, pH value to 4.5 ~ 5.0 of the reaction solution of set-up procedure three, then zytase and xylobiase is added, temperature be 50 DEG C, under rotating speed is the condition of 150rpm, enzymolysis 48h, add cellulase and beta-glucosidase again, temperature be 50 DEG C, under rotating speed is the condition of 150rpm, enzymolysis 24h, obtains glucose and xylose; Wherein, zytase and xylobiase addition are 2400U/g Mierocrystalline cellulose, and the addition of cellulase is 15FPU/g Mierocrystalline cellulose, and beta-glucosidase addition is 15CBU/g Mierocrystalline cellulose.
Embodiment two: present embodiment and embodiment one unlike: after the solid formation after drying in step 2 is mixed according to the ratio that solid-to-liquid ratio is 1g:9mL with water described in step 3.Other is identical with embodiment one.
Content of the present invention is not limited only to the content of the respective embodiments described above, and the combination of one of them or several embodiment equally also can realize the object of inventing.
Beneficial effect of the present invention is verified by following examples:
Embodiment 1
The a kind of of the present embodiment improves the method that maize straw enzymolysis efficiency also obtains xylose and glucose simultaneously, and it carries out according to following steps:
Maize straw is ground to 0.250 ~ 0.425mm, by U.S.'s renewable energy source laboratory (National Renewable Energy Laboratory, NREL) method measures maize straw composition following (with dry weight basis): Mierocrystalline cellulose 43.4%, hemicellulose 21.2%, xylogen 18.3%, extract 13.5%.
Maize straw and mass percentage are that 5% ethanol-water solution mixes with solid-to-liquid ratio 1:15, room temperature, 120r/min, and jolting 24h, obtains solid product through solid-liquid separation, dry solid product 12h for 35 DEG C.Measure extract by U.S.'s renewable energy source laboratory method and be removed 91.5%.
The solid product of having dried is transferred in hydrothermal reaction kettle after mixing with solid-to-liquid ratio 2.63g:23.7mL with water, is heated by reactor to 180 DEG C, maintains 20min, is then immersed in mixture of ice and water by reactor immediately and terminate hydro-thermal reaction.
Solidliquid mixture after twice hydrothermal pretreatment is poured in 100mL Erlenmeyer flask.
6mol/LHCl adjusted to ph is dripped to 4.5-5.0 in solidliquid mixture.
In solidliquid mixture, add zytase and xylobiase, addition is 2400U/g Mierocrystalline cellulose, 50 DEG C, 150rpm, enzymolysis 48h.
In solidliquid mixture, add cellulase and beta-glucosidase, addition is 15FPU+15CBU/g Mierocrystalline cellulose, 50 DEG C, 150rpm, and enzymolysis 24h, obtains glucose and xylose.Cellulosic enzymatic hydrolyzation and wood sugar output are in table 1.
The present embodiment removal extract carries out the maize straw scanning electron microscopic picture after the hydrothermal treatment consists after hydrothermal pretreatment as shown in Figure 1, and as shown in Figure 1, stalk surface is more smooth does not have obvious sediment to adhere to.
Embodiment 2
The a kind of of the present embodiment improves the method that maize straw enzymolysis efficiency also obtains xylose and glucose simultaneously, and it carries out according to following steps:
Maize straw is ground to 0.250-0.425mm, by U.S.'s renewable energy source laboratory (National Renewable Energy Laboratory, NREL) method measures maize straw composition following (with dry weight basis): Mierocrystalline cellulose 43.4%, hemicellulose 21.2%, xylogen 18.3%, extract 13.5%.
Maize straw and mass percentage are that 5% ethanol-water solution mixes with solid-to-liquid ratio 1:15, room temperature, 120r/min, and jolting 24h, obtains solid product through solid-liquid separation, dry solid product 12h for 35 DEG C.Measure extract by U.S.'s renewable energy source laboratory method and be removed 91.5%.
The solid product of having dried is transferred in hydrothermal reaction kettle after mixing with solid-to-liquid ratio 2.63g:23.7mL with water, is heated by reactor to 190 DEG C, maintains 20min, is then immersed in mixture of ice and water by reactor immediately and terminate hydro-thermal reaction.
Solidliquid mixture after twice hydrothermal pretreatment is poured in 100mL Erlenmeyer flask.
6mol/LHCl adjusted to ph is dripped to 4.5-5.0 in solidliquid mixture.
In solidliquid mixture, add zytase and xylobiase, addition is 2400U/g Mierocrystalline cellulose, 50 DEG C, 150rpm, enzymolysis 48h.
In solidliquid mixture, add cellulase and beta-glucosidase, addition is 15FPU+15CBU/g Mierocrystalline cellulose, 50 DEG C, 150rpm, and enzymolysis 24h, obtains glucose and xylose.Cellulosic enzymatic hydrolyzation and wood sugar output are in table 1.
The present embodiment removal extract carries out the maize straw scanning electron microscopic picture after the hydrothermal treatment consists after hydrothermal pretreatment as shown in Figure 3, and as shown in Figure 3, stalk surface is more smooth does not have obvious sediment to adhere to.
Embodiment 3:
The a kind of of the present embodiment improves the method that maize straw enzymolysis efficiency also obtains xylose and glucose simultaneously, and it carries out according to following steps:
Maize straw is ground to 0.250-0.425mm, by U.S.'s renewable energy source laboratory (National Renewable Energy Laboratory, NREL) method measures maize straw composition following (with dry weight basis): Mierocrystalline cellulose 43.4%, hemicellulose 21.2%, xylogen 18.3%, extract 13.5%.
Maize straw mixes with solid-to-liquid ratio 1:15 with 5% ethanol-water solution, room temperature, 120r/min, and jolting 24h, obtains solid product through solid-liquid separation, dries solid product 12h for 35 DEG C.Measure extract by U.S.'s renewable energy source laboratory method and be removed 91.5%.
The solid product of having dried is transferred in hydrothermal reaction kettle after mixing with solid-to-liquid ratio 2.63g:23.7mL with water, is heated by reactor to 200 DEG C, maintains 20min, is then immersed in mixture of ice and water by reactor immediately and terminate hydro-thermal reaction.
Solidliquid mixture after twice hydrothermal pretreatment is poured in 100mL Erlenmeyer flask.
In solidliquid mixture, drip concentration is that the HCl solution adjusted to ph of 6mol/L is to 4.5-5.0.
In solidliquid mixture, add zytase and xylobiase, addition is 2400U/g Mierocrystalline cellulose, 50 DEG C, 150rpm, enzymolysis 48h.
In solidliquid mixture, add cellulase and beta-glucosidase, addition is 15FPU+15CBU/g Mierocrystalline cellulose, 50 DEG C, 150rpm, and enzymolysis 24h, obtains glucose and xylose.Cellulosic enzymatic hydrolyzation and wood sugar output are in table 1.
The present embodiment removal extract carries out the maize straw scanning electron microscopic picture after the hydrothermal treatment consists after hydrothermal pretreatment as shown in Figure 5, and as shown in Figure 5, stalk surface is more smooth does not have obvious sediment to adhere to.
Comparative example 1:
Maize straw is ground to 0.250-0.425mm, by U.S.'s renewable energy source laboratory (National Renewable Energy Laboratory, NREL) method measures maize straw composition following (with dry weight basis): Mierocrystalline cellulose 43.4%, hemicellulose 21.2%, xylogen 18.3%, extract 13.5%.
Maize straw is transferred in hydrothermal reaction kettle after mixing with solid-to-liquid ratio 3g:23.7mL with water after drying 12h through 35 DEG C, is heated by reactor to 180 DEG C, maintains 20min, is then immersed in mixture of ice and water by reactor immediately and terminate hydro-thermal reaction.
Solidliquid mixture after separation and culture, solid phase is dried after 12h as enzymolysis raw material through 35 DEG C, cellulase and beta-glucosidase is added in the citric acid-sodium citrate damping fluid (enzymolysis raw material and damping fluid solid-to-liquid ratio are 1:9) of pH=4.8, enzyme dosage is 15FPU+15CBU/g Mierocrystalline cellulose, 50 DEG C, 150rpm, enzymolysis 48h.Cellulosic enzymatic hydrolyzation is in table 1.
The maize straw Electronic Speculum figure of the present embodiment after hydrothermal treatment consists as shown in Figure 2, as shown in Figure 2, stalk surface all has precipitates attachment (extract is formed in pre-treatment) in a large number, and these precipitations can reduce cellulosic contact area in cellulase and stalk thus reduce enzymolysis efficiency.
Comparative example 2:
Maize straw is ground to 0.250-0.425mm, by U.S.'s renewable energy source laboratory (National Renewable Energy Laboratory, NREL) method measures maize straw composition following (with dry weight basis): Mierocrystalline cellulose 43.4%, hemicellulose 21.2%, xylogen 18.3%, extract 13.5%.
Maize straw is transferred in hydrothermal reaction kettle after mixing with solid-to-liquid ratio 3g:23.7mL with water after drying 12h through 35 DEG C, is heated by reactor to 190 DEG C, maintains 20min, is then immersed in mixture of ice and water by reactor immediately and terminate hydro-thermal reaction.
Solidliquid mixture after separation and culture, solid phase is dried after 12h as enzymolysis raw material through 35 DEG C, cellulase and beta-glucosidase is added in the citric acid-sodium citrate damping fluid (enzymolysis raw material and damping fluid solid-to-liquid ratio are 1:9) of pH=4.8, enzyme dosage is 15FPU+15CBU/g Mierocrystalline cellulose, 50 DEG C, 150rpm, enzymolysis 48h.Cellulosic enzymatic hydrolyzation is in table 1.
The maize straw Electronic Speculum figure of the present embodiment after hydrothermal treatment consists as shown in Figure 4, as shown in Figure 4, stalk surface all has precipitates attachment (extract is formed in pre-treatment) in a large number, and these precipitations can reduce cellulosic contact area in cellulase and stalk thus reduce enzymolysis efficiency.
Comparative example 3:
Maize straw is ground to 0.250-0.425mm, by U.S.'s renewable energy source laboratory (National Renewable Energy Laboratory, NREL) method measures maize straw composition following (with dry weight basis): Mierocrystalline cellulose 43.4%, hemicellulose 21.2%, xylogen 18.3%, extract 13.5%.
Maize straw is transferred in hydrothermal reaction kettle after mixing with solid-to-liquid ratio 3g:23.7mL with water after drying 12h through 35 DEG C, is heated by reactor to 200 DEG C, maintains 20min, is then immersed in mixture of ice and water by reactor immediately and terminate hydro-thermal reaction.
Solidliquid mixture after separation and culture, solid phase is dried after 12h as enzymolysis raw material through 35 DEG C, cellulase and beta-glucosidase is added in the citric acid-sodium citrate damping fluid (enzymolysis raw material and damping fluid solid-to-liquid ratio are 1:9) of pH=4.8, enzyme dosage is 15FPU+15CBU/g Mierocrystalline cellulose, 50 DEG C, 150rpm, enzymolysis 48h.Cellulosic enzymatic hydrolyzation is in table 1.
The maize straw Electronic Speculum figure of the present embodiment after hydrothermal treatment consists as shown in Figure 6, as shown in Figure 6, stalk surface all has precipitates attachment (extract is formed in pre-treatment) in a large number, and these precipitations can reduce cellulosic contact area in cellulase and stalk thus reduce enzymolysis efficiency.
The each embodiment of table 1 and comparative example experimental result
| Embodiment and comparative example | Embodiment 1 | Comparative example 1 | Embodiment 2 | Comparative example 2 | Embodiment 3 | Comparative example 3 |
| Cellulase hydrolysis rate (%) | 43.5 | 30.4 | 62.1 | 46.8 | 85.1 | 64.4 |
| Wood sugar output (mg/ml) | 21.3 | 3.39 | 21.7 | 1.99 | 22.6 | 0.49 |
As can be seen from Table 1, employing the inventive method (embodiment) afterwards cellulase hydrolysis rate, all higher than the cellulase hydrolysis rate in comparative example (ortho-water Grape berry+solid-liquid separation+cellulase hydrolysis), illustrates that the inventive method can improve hydrothermal pretreatment effect.In addition, compared with ortho-water Grape berry technique, after adopting the inventive method, obtain the wood sugar of higher concentration.
Claims (2)
1. improve the method that maize straw enzymolysis efficiency also obtains xylose and glucose simultaneously, it is characterized in that it carries out according to following steps:
One, cut off by maize straw, being then crushed to granularity is 0.250 ~ 0.425mm;
Two, be then that 5% ethanolic soln mixes with the ratio of solid-to-liquid ratio 1:15 by the maize straw after pulverizing in step one with mass percentage, under room temperature, rotating speed are the condition of 150r/min, jolting 24h, then through solid-liquid separation, collect solid formation, solid formation is dried 12h under 35 DEG C of conditions;
Three, after the solid formation after oven dry in step 2 being mixed according to the ratio that solid-to-liquid ratio is 1g:8 ~ 12mL with water, be transferred in hydrothermal reaction kettle, reactor is heated to 180 ~ 200 DEG C, after maintaining 20min, reactor is immersed in mixture of ice and water and terminates hydro-thermal reaction;
Four, pH value to 4.5 ~ 5.0 of the reaction solution of set-up procedure three, then zytase and xylobiase is added, temperature be 50 DEG C, under rotating speed is the condition of 150rpm, enzymolysis 48h, add cellulase and beta-glucosidase again, temperature be 50 DEG C, under rotating speed is the condition of 150rpm, enzymolysis 24h, obtains glucose and xylose; Wherein, zytase and xylobiase addition are 2400U/g Mierocrystalline cellulose, and the addition of cellulase is 15FPU/g Mierocrystalline cellulose, and beta-glucosidase addition is 15CBU/g Mierocrystalline cellulose.
2. according to claim 1ly a kind ofly improve maize straw enzymolysis efficiency and obtain the method for xylose and glucose simultaneously, after it is characterized in that being mixed according to the ratio that solid-to-liquid ratio is 1g:9mL with water by the solid formation after drying in step 2 described in step 3.
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Cited By (6)
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| CN107012182A (en) * | 2017-05-09 | 2017-08-04 | 陕西兆信生物科技有限公司 | The method that xylo-oligosaccharide, lignin and glucose are extracted using walnut shell |
| CN110066840A (en) * | 2019-03-08 | 2019-07-30 | 华南农业大学 | A kind of method that hydro-thermal-microwave coupled ion liquid two-step method lignocellulosic material improves enzymolysis efficiency |
| CN113416760A (en) * | 2021-07-09 | 2021-09-21 | 河南农业大学 | Method for promoting hydrolysis of lignocellulose by heat treatment |
| CN114045312A (en) * | 2021-11-22 | 2022-02-15 | 国网内蒙古东部电力有限公司电力科学研究院 | Corn straw xylo-oligosaccharide and biogas co-production method |
| CN115341004A (en) * | 2021-05-12 | 2022-11-15 | 国投生物科技投资有限公司 | Method for preparing monosaccharide from biomass raw material |
| CN117187318A (en) * | 2023-07-26 | 2023-12-08 | 华南农业大学 | Method for preparing fermentable sugar solution by corn stalk enzymolysis |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012182A (en) * | 2017-05-09 | 2017-08-04 | 陕西兆信生物科技有限公司 | The method that xylo-oligosaccharide, lignin and glucose are extracted using walnut shell |
| CN110066840A (en) * | 2019-03-08 | 2019-07-30 | 华南农业大学 | A kind of method that hydro-thermal-microwave coupled ion liquid two-step method lignocellulosic material improves enzymolysis efficiency |
| CN115341004A (en) * | 2021-05-12 | 2022-11-15 | 国投生物科技投资有限公司 | Method for preparing monosaccharide from biomass raw material |
| CN113416760A (en) * | 2021-07-09 | 2021-09-21 | 河南农业大学 | Method for promoting hydrolysis of lignocellulose by heat treatment |
| CN113416760B (en) * | 2021-07-09 | 2024-03-01 | 河南农业大学 | Method for promoting lignocellulose hydrolysis by heat treatment |
| CN114045312A (en) * | 2021-11-22 | 2022-02-15 | 国网内蒙古东部电力有限公司电力科学研究院 | Corn straw xylo-oligosaccharide and biogas co-production method |
| CN114045312B (en) * | 2021-11-22 | 2023-09-26 | 国网内蒙古东部电力有限公司电力科学研究院 | Corn straw xylooligosaccharide and methane co-production method |
| CN117187318A (en) * | 2023-07-26 | 2023-12-08 | 华南农业大学 | Method for preparing fermentable sugar solution by corn stalk enzymolysis |
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