CN105036354B - A kind of biological restoration device at oil pollution sandy beach - Google Patents
A kind of biological restoration device at oil pollution sandy beach Download PDFInfo
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- CN105036354B CN105036354B CN201510455134.7A CN201510455134A CN105036354B CN 105036354 B CN105036354 B CN 105036354B CN 201510455134 A CN201510455134 A CN 201510455134A CN 105036354 B CN105036354 B CN 105036354B
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Abstract
The invention belongs to littoral zone oil pollution technical field of biological remediation, relate to the biological restoration device at a kind of oil pollution sandy beach, upper strata silk screen is built-in with Solid nutritional agent and graininess microbial inoculum；The aperture of upper strata silk screen and lower floor's silk screen is 2mm, upper strata silk screen is bulge-structure, lower floor's silk screen is planar structure, lower floor's silk screen cover upper strata silk screen after upside down between upper strata porous PVC gripper shoe and lower floor's porous PVC gripper shoe, plastic layer is put in plastic frame after upper strata porous PVC gripper shoe and lower floor porous PVC gripper shoe being compressed；Its simple in construction, easy to operate, principle is reliable, and repair rate is high, and environmental friendliness, low cost, for oil pollution sandy beach or the biological restoration of coastal tidal.
Description
Technical field:
The invention belongs to littoral zone oil pollution technical field of biological remediation, the biology relating to a kind of oil pollution sandy beach is repaiied
Apparatus for coating, installs biological restoration device in the beach body at oil pollution sandy beach, by adding petroleum hydrocarbon in biological restoration device
Degradation bacterial agent and nutrient, make petroleum hydrocarbon degradation bacterium can be fully contacted with the petroleum pollution in the body of beach and it quickly be removed,
Reach the effect of oil pollution sandy beach fast quick-recovery cleaning.
Background technology:
Marine oil overflow and Lu Yuan blowdown made littoral zone suffer severe contamination, such as Concophillips Company Peng Lai 19-3 oil in 2011
Field oil spill accident causes 870 square kilometres of sea water heavily contaminated, and contaminated area sediment pollution area is 1600 squares
Kilometer, the marine ecology caused infringement is worth and amounts to 16.83 hundred million yuans.The oil of oil spill accident leakage can penetrate into sandy beach
The 15-25cm degree of depth, have even can penetrate into 60cm, the degree of depth of infiltration and speed depend on overflow oil viscosity, volume and
The porosity of sandy beach deposit.Littoral zone naturally occurs petroleum hydrocarbon degradation bacterium.Oil spilling is the carbon source that microorganism provides abundance,
Supplement nitrogen at Polluted area, phosphorus can promote the growth of petroleum hydrocarbon degradation bacterium, and this is traditional biological restoration, with physics,
Chemical redemption is compared, and biological restoration efficiency is high, spend low and non-secondary pollution, is applied at multiple oil spilling scenes.So
And, owing to sandy beach can be acted on by wave, ocean current, the nutrient of applying is likely to just not made full use of by petroleum hydrocarbon degradation bacterium
It is lost in marine environment, brings the threat of eutrophication；Original inhabitants' petroleum hydrocarbon degradation bacterium shortage acts on difficult degradation oil pollution
The enzyme system of thing, low to the clearance of difficult degradation petroleum pollution；The efficient degrading bacteria added to littoral zone is also faced with microbial inoculum stream
The problem lost, all there is limitation in the most above-mentioned several ways.Therefore, the biological restoration dress at a kind of oil pollution sandy beach is sought
Put, by installing biological restoration device on the beach body at oil pollution sandy beach, and in device, add immobilization highly effective petroleum hydrocarbon
Degradation bacteria, is aided with spacetabs type nutrient simultaneously, make petroleum hydrocarbon degradation bacterium can and sandy beach on petroleum pollution be fully contacted and incite somebody to action
It is quickly removed, and reaches the effect of oil pollution sandy beach fast quick-recovery cleaning.
Summary of the invention:
It is an object of the invention to the shortcoming overcoming prior art to exist, seeking design provides a kind of oil pollution sandy beach raw
Thing prosthetic device, can make oil pollution sandy beach obtain fast quick-recovery within the short period (30～60 days) by this device and clean,
Complete biological restoration process.
To achieve these goals, the agent structure of the present invention includes that upper strata porous PVC gripper shoe, lower floor's porous PVC prop up
Fagging, upper strata silk screen, lower floor's silk screen, plastic frame, plastic layer, Solid nutritional agent and graininess microbial inoculum；Upper strata porous PVC props up
Fagging is rectangle bulge-structure, and lower floor's porous PVC gripper shoe is slab construction, upper strata porous PVC gripper shoe and lower floor's porous
The aperture of PVC gripper shoe is 10-15mm；Upper strata silk screen is built-in with Solid nutritional agent and graininess microbial inoculum；Employing rustless steel gold
Upper strata silk screen and the aperture of lower floor's silk screen that genus material is made are 2mm, and upper strata silk screen is bulge-structure, and lower floor's silk screen is plane
Structure, lower floor's silk screen cover after the silk screen of upper strata upside down upper strata porous PVC gripper shoe and lower floor's porous PVC gripper shoe it
Between, plastic layer puts into formation oil in plastic frame after upper strata porous PVC gripper shoe and lower floor's porous PVC gripper shoe being compressed
Pollute sandy beach biological restoration device.
The detailed process that the present invention uses oil pollution sandy beach biological restoration device to realize biological restoration comprises the following steps:
(1), determine graininess microbial inoculum and the addition of Solid nutritional agent, use immobilized particle diameter to be more than the efficient of 2mm
Petroleum hydrocarbon degradation microbial inoculum determines according to the operation instruction of microbial inoculum as graininess microbial inoculum, its addition；Use commercially available slow release agriculture fertile
As Solid nutritional agent, the nitrogenous fertilizer added in Solid nutritional agent, phosphate fertilizer amount make the mass ratio of C:N:P be 100:10:1, and wherein C is
Pollute the total C amount contained in petroleum hydrocarbon on sandy beach；Or use NaNO3、KH2PO4Prepare voluntarily according to the quality of above-mentioned C:N:P,
And utilize prior art to obtain Solid nutritional agent after pelletize, slow release process；
(2), on oil pollution sandy beach, determine contaminated area and the degree of depth, determine that biological restoration fills according to pollution depth
The height put, then using 2 times of length values as biological restoration device of biological restoration device height, determines single biological restoration
The area of device；
(3) the upper strata silk screen of biological restoration device is loaded with gauze after, Solid nutritional agent and graininess microbial inoculum being wrapped up
High spot, and it is inverted in the middle of upper strata porous PVC gripper shoe and lower floor's porous PVC gripper shoe, with moulding after covering lower floor's silk screen
Upper strata porous PVC gripper shoe is compressed by material press strip, is then charged in plastic frame fixing；
(4), according to step (1) needs Solid nutritional agent and graininess microbial inoculum total amount and each biological restoration added
Solid nutritional agent in device and graininess microbial inoculum addition, determine the biological restoration device number of needs；
(5), contaminated Intertidal zone is divided into latticed, spaced principle staggered according to biological restoration device,
Vertically digging out a lot of groove on oil pollution sandy beach on contaminated Intertidal zone, the degree of depth of groove is that biological prosthetic device is high
1.1-1.5 times of degree, then imbeds Intertidal zone by biological restoration device；
(6), determine to be a cycle of operation 15 days, in cycle of operation, measured in the Interstitial Water of sandy beach every 3 days total respectively
Petroleum hydrocarbon and the quantity of petroleum hydrocarbon degradation bacterium, run all after date, becomes according to the quantity of total petroleum hydrocarbon and petroleum hydrocarbon degradation bacterium
Change situation, adjusts or supplements the microbial inoculum in biological restoration device and Solid nutritional dosage；
(7), 2-4 week after date, stop biological restoration process, then by biological restoration device take out recycling, it is achieved
The biological restoration at oil pollution sandy beach.
The present invention compared with prior art, its simple in construction, easy to operate, principle is reliable, and repair rate is high, environmental friendliness,
Low cost, for oil pollution sandy beach or the biological restoration of coastal tidal.
Accompanying drawing illustrates:
Fig. 1 is the agent structure principle schematic of the present invention.
Fig. 2 is the plan structure principle schematic of the present invention.
Fig. 3 is that the embodiment of the present invention uses in the matched group that the test group and not carrying out of biological restoration device processes, gap
Water Petroleum Hydrocarbon concentration (a) and the number change situation of petroleum hydrocarbon degradation bacterium (b).
Detailed description of the invention:
Below by embodiment and combine accompanying drawing the invention will be further described.
The agent structure of oil pollution sandy beach biological restoration device described in the present embodiment include upper strata porous PVC gripper shoe 1,
Lower floor's porous PVC gripper shoe 2, upper strata silk screen 3, lower floor's silk screen 4, plastic frame 5, plastic layer 6, Solid nutritional agent 7 and granule
Shape microbial inoculum 8；Upper strata porous PVC gripper shoe 1 is rectangle bulge-structure, and lower floor's porous PVC gripper shoe 2 is slab construction, upper strata
The aperture of porous PVC gripper shoe 1 and lower floor's porous PVC gripper shoe 2 is 10-15mm；Solid nutritional agent 7 He in upper strata silk screen 3
Graininess microbial inoculum 8；Upper strata silk screen 3 and the aperture of lower floor's silk screen 4 that employing stainless steel metal material is made are 2mm, upper strata silk
Net 3 is bulge-structure, and lower floor's silk screen 4 is planar structure, lower floor's silk screen 4 cover upper strata silk screen 3 after upside down in upper strata porous
Between PVC gripper shoe 1 and lower floor's porous PVC gripper shoe 2, plastic layer 6 is by upper strata porous PVC gripper shoe 1 and lower floor's porous PVC
Gripper shoe 2 is put into after compressing in plastic frame 5 and is formed oil pollution sandy beach biological restoration device.
The present embodiment realizes the biological restoration to oil pollution sandy beach in the biological restoration device of oil pollution sandy beach, specifically
Comprise the following steps:
(1), determine graininess microbial inoculum 8 and the addition of Solid nutritional agent 7, use the immobilized particle diameter height more than 2mm
Effect petroleum hydrocarbon degradation microbial inoculum determines according to the operation instruction of highly effective petroleum alkane degradation microbial inoculum as graininess microbial inoculum 8, its addition；
Using commercially available slow release agriculture fertile as Solid nutritional agent 7, the nitrogenous fertilizer added in Solid nutritional agent 7, phosphate fertilizer amount make the mass ratio of C:N:P
For 100:10:1, wherein C is to pollute the total C amount contained in petroleum hydrocarbon on sandy beach；Or use NaNO3、KH2PO4According to above-mentioned C:
The quality of N:P is prepared voluntarily, and utilizes prior art to obtain Solid nutritional agent 7 after pelletize, slow release process；
(2), on oil pollution sandy beach, determine contaminated area and the degree of depth, determine that biological restoration fills according to pollution depth
The height put, then using 2 times of length values as biological restoration device of biological restoration device height, determines single biological restoration
The area of device；
(3) the upper strata silk screen 3 of biological restoration device is loaded with gauze after, Solid nutritional agent 7 and graininess microbial inoculum 8 being wrapped up
High spot, and be inverted into after covering lower floor's silk screen 4 in the middle of upper strata porous PVC gripper shoe 1 and lower floor's porous PVC gripper shoe 2,
With plastic layer 6, upper strata porous PVC gripper shoe 1 is compressed, be then charged in plastic frame 5 fixing；
(4), according to step (1) needing the Solid nutritional agent 7 added and graininess microbial inoculum 8 total amount and each biology repair
Solid nutritional agent 7 in apparatus for coating and graininess microbial inoculum 8 addition, determine the biological restoration device number of needs；
(5), contaminated Intertidal zone is divided into latticed, spaced principle staggered according to biological restoration device,
Vertically digging out a lot of groove on oil pollution sandy beach on contaminated Intertidal zone, the degree of depth of groove is that biological prosthetic device is high
1.1-1.5 times of degree, then imbeds Intertidal zone by biological restoration device；
(6), determine to be a cycle of operation 15 days, in cycle of operation, measured in the Interstitial Water of sandy beach every 3 days total respectively
Petroleum hydrocarbon and the quantity of petroleum hydrocarbon degradation bacterium, run all after date, becomes according to the quantity of total petroleum hydrocarbon and petroleum hydrocarbon degradation bacterium
Change situation, adjusts or supplements the microbial inoculum in biological restoration device and the amount of Solid nutritional agent；
(7), 2-4 week after date, stop biological restoration process, then by biological restoration device take out recycling, it is achieved
The biological restoration at oil pollution sandy beach.
Embodiment 1:
The present embodiment has carried out contrast test on oil pollution sandy beach, and sandy beach is divided into 2 blocks, respectively test group
And matched group.Matched group does not make any process, and test group uses biological restoration device to process, first according to the oil on sandy beach
Hydrocarbon C content calculates N, P nutrition of needs, prepares spacetabs type N, P nutrient；Immobilization highly effective petroleum surfactant hydrocarbon degradation bacteria is used to make
For microbial inoculum, determine applied amount according to the usefulness of microbial inoculum, then nutrient and microbial inoculum are added in biological restoration device, by the dirt at sandy beach
Dye region is divided into latticed, and biological restoration device is spaced, is staggered；Test carries out 30d, measures gap, sandy beach every 3d
Total petroleum hydrocarbon concentration in water and the quantity of petroleum hydrocarbon degradation bacterium, as it is shown on figure 3, it can be seen that use biological restoration
After device, the total petroleum hydrocarbon concentration in Interstitial Water is significantly lower than matched group, and the quantity of petroleum hydrocarbon degradation bacterium is apparently higher than being not added with
The matched group processed.
Claims (2)
1. the biological restoration device at an oil pollution sandy beach, it is characterised in that agent structure include upper strata porous PVC gripper shoe,
Lower floor's porous PVC gripper shoe, upper strata silk screen, lower floor's silk screen, plastic frame, plastic layer, Solid nutritional agent and graininess microbial inoculum；
Upper strata porous PVC gripper shoe is rectangle bulge-structure, and lower floor's porous PVC gripper shoe is slab construction, and upper strata porous PVC supports
The aperture of plate and lower floor's porous PVC gripper shoe is 10-15mm；Upper strata silk screen is built-in with Solid nutritional agent and graininess microbial inoculum,
Graininess microbial inoculum uses the immobilized particle diameter highly effective petroleum alkane degradation microbial inoculum more than 2mm；Stainless steel metal material is used to make
Upper strata silk screen and the aperture of lower floor's silk screen be 2mm, upper strata silk screen is bulge-structure, and lower floor's silk screen is planar structure, lower floor
Silk screen cover upper strata silk screen after upside down between upper strata porous PVC gripper shoe and lower floor's porous PVC gripper shoe, plastic layer
Put into after upper strata porous PVC gripper shoe and lower floor's porous PVC gripper shoe are compressed in plastic frame and form the life of oil pollution sandy beach
Thing prosthetic device.
The biological restoration device at oil pollution sandy beach the most according to claim 1, it is characterised in that use this device to oil
Pollution sandy beach carries out the detailed process of biological restoration:
(1), determine graininess microbial inoculum and the addition of Solid nutritional agent, use the immobilized particle diameter highly effective petroleum more than 2mm
Alkane degradation microbial inoculum determines according to the operation instruction of microbial inoculum as graininess microbial inoculum, its addition；Use commercially available slow release agriculture fertilizer conduct
Solid nutritional agent, the nitrogenous fertilizer added in Solid nutritional agent, phosphate fertilizer amount make the mass ratio of C:N:P be 100:10:1, and wherein C is for polluting
The total C amount contained in petroleum hydrocarbon on sandy beach；Or use NaNO3、KH2PO4Prepare voluntarily according to the quality of above-mentioned C:N:P, and profit
After pelletize, slow release process, Solid nutritional agent is obtained by prior art；
(2), on oil pollution sandy beach, determine contaminated area and the degree of depth, determine biological restoration device according to pollution depth
Highly, then using 2 times of length values as biological restoration device of biological restoration device height, single biological restoration device is determined
Area；
(3) projection of the upper strata silk screen of biological restoration device is loaded with gauze after, Solid nutritional agent and graininess microbial inoculum being wrapped up
Place, and be inverted in the middle of upper strata porous PVC gripper shoe and lower floor's porous PVC gripper shoe after covering lower floor's silk screen, use plastics pressure
Upper strata porous PVC gripper shoe is compressed by bar, is then charged in plastic frame fixing；
(4), according to step (1) needs the Solid nutritional agent and graininess microbial inoculum total amount and each biological restoration device that add
In Solid nutritional agent and graininess microbial inoculum addition, determine the biological restoration device number of needs；
(5), being divided into latticed by contaminated Intertidal zone, spaced principle staggered according to biological restoration device, at stone
Vertically digging out a lot of groove on contaminated Intertidal zone on greasy dirt dye sandy beach, the degree of depth of groove is biological prosthetic device height
1.1-1.5 times, then biological restoration device is imbedded Intertidal zone；
(6), determine to be a cycle of operation 15 days, in cycle of operation, measured total oil in the Interstitial Water of sandy beach respectively every 3 days
Hydrocarbon and the quantity of petroleum hydrocarbon degradation bacterium, run all after date, according to the number change feelings of total petroleum hydrocarbon and petroleum hydrocarbon degradation bacterium
Condition, adjusts or supplements the microbial inoculum in biological restoration device and Solid nutritional dosage；
(7), 2-4 week after date, stop biological restoration process, then by biological restoration device take out recycling, it is achieved oil
Pollute the biological restoration at sandy beach.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002205092A (en) * | 2001-01-15 | 2002-07-23 | Ryoichi Tsumoto | Plantation for water cleaning |
| RU2008123891A (en) * | 2008-06-18 | 2009-12-27 | Екатерина Анатольевна Сребняк (RU) | CARTRIDGE FOR CLEANING NATURAL WATERS FROM OIL CONTAMINATIONS |
| CN102021132A (en) * | 2009-12-03 | 2011-04-20 | 北京航空航天大学 | Method for screening and remediation of petroleum-contaminated soil bioremediation agent |
| CN102249428A (en) * | 2011-06-10 | 2011-11-23 | 吉林大学 | In-situ remediation method of petroleum-polluted groundwater |
| CN102491494A (en) * | 2011-11-24 | 2012-06-13 | 北京师范大学 | Simulator for adsorption and strengthened microbiological degradation of marine spilled oil pollution |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110315628A1 (en) * | 2010-06-24 | 2011-12-29 | Shaw Mark D | System and method for capturing and bioremediating hydrocarbon pollutants using dynamic filters |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002205092A (en) * | 2001-01-15 | 2002-07-23 | Ryoichi Tsumoto | Plantation for water cleaning |
| RU2008123891A (en) * | 2008-06-18 | 2009-12-27 | Екатерина Анатольевна Сребняк (RU) | CARTRIDGE FOR CLEANING NATURAL WATERS FROM OIL CONTAMINATIONS |
| CN102021132A (en) * | 2009-12-03 | 2011-04-20 | 北京航空航天大学 | Method for screening and remediation of petroleum-contaminated soil bioremediation agent |
| CN102249428A (en) * | 2011-06-10 | 2011-11-23 | 吉林大学 | In-situ remediation method of petroleum-polluted groundwater |
| CN102491494A (en) * | 2011-11-24 | 2012-06-13 | 北京师范大学 | Simulator for adsorption and strengthened microbiological degradation of marine spilled oil pollution |
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