CN105036354A - Bioremediation device of petroleum-polluted sand beach - Google Patents
Bioremediation device of petroleum-polluted sand beach Download PDFInfo
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- CN105036354A CN105036354A CN201510455134.7A CN201510455134A CN105036354A CN 105036354 A CN105036354 A CN 105036354A CN 201510455134 A CN201510455134 A CN 201510455134A CN 105036354 A CN105036354 A CN 105036354A
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- biological restoration
- silk screen
- pad
- upper strata
- restoration device
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- 239000004576 sand Substances 0.000 title abstract 2
- 239000003208 petroleum Substances 0.000 claims abstract description 42
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 36
- 239000007787 solid Substances 0.000 claims abstract description 31
- 239000003795 chemical substances by application Substances 0.000 claims description 37
- 235000016709 nutrition Nutrition 0.000 claims description 36
- 239000003209 petroleum derivative Substances 0.000 claims description 29
- 230000015556 catabolic process Effects 0.000 claims description 24
- 238000006731 degradation reaction Methods 0.000 claims description 24
- 241000894006 Bacteria Species 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 150000001335 aliphatic alkanes Chemical class 0.000 claims description 4
- 238000010276 construction Methods 0.000 claims description 3
- 238000005469 granulation Methods 0.000 claims description 3
- 230000003179 granulation Effects 0.000 claims description 3
- 239000007769 metal material Substances 0.000 claims description 3
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000002686 phosphate fertilizer Substances 0.000 claims description 3
- 238000004064 recycling Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 150000002430 hydrocarbons Chemical class 0.000 description 2
- 239000003305 oil spill Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of petroleum pollution bioremediation in coastal zones, and relates to a bioremediation device for petroleum-polluted beaches, wherein a solid nutrient and a granular microbial inoculum are arranged in an upper-layer screen; the aperture of the upper layer of silk screen and the aperture of the lower layer of silk screen are both 2mm, the upper layer of silk screen is of a convex structure, the lower layer of silk screen is of a planar structure, the lower layer of silk screen covers the upper layer of silk screen and is inversely placed between the upper layer of porous PVC supporting plate and the lower layer of porous PVC supporting plate, and the upper layer of porous PVC supporting plate and the lower layer of porous PVC supporting plate are tightly pressed by the plastic pressing strip and then are placed in the plastic frame; the device has the advantages of simple structure, convenient operation, reliable principle, high restoration rate, environmental protection and low cost, and is used for the biological restoration of the oil-polluted sand beach or coastal beach.
Description
Technical field:
The invention belongs to littoral zone petroleum pollution technical field of biological remediation, relate to a kind of biological restoration device of petroleum pollution sandy beach, in the beach body at petroleum pollution sandy beach, biological restoration device is installed, by adding petroleum hydrocarbon degradation microbial inoculum and nutrition agent in biological restoration device, make petroleum hydrocarbon degradation bacterium fully contact with the petroleum pollution in the body of beach and it to be removed fast, reach the effect that the fast quick-recovery in petroleum pollution sandy beach is clean.
Background technology:
Marine oil overflow and Lu Yuan blowdown make littoral zone suffer severe contamination, as 19-3 oil field, Concophillips Company Peng Lai in 2011 oil spill accident causes 870 square kilometres of seawater to be subject to severe contamination, contaminated area sediment pollution area is 1600 square kilometres, and the marine ecology infringement caused is worth total 16.83 hundred million yuans.The oil that oil spill accident leaks can infiltrate the sandy beach 15-25cm degree of depth, and what have even can penetrate into 60cm, and the degree of depth of infiltration and speed depend on the sedimental porosity in excessive oil viscosity, volume and sandy beach.Littoral zone is natural also exists petroleum hydrocarbon degradation bacterium.Oil spilling is that microorganism provides sufficient carbon source, in the growth that Polluted area supplements nitrogen, phosphorus can promote petroleum hydrocarbon degradation bacterium, this is traditional biological restoration, compared with physics, chemical redemption, biological restoration efficiency is high, it is low to spend and non-secondary pollution, is applied at multiple oil spilling scene.But because sandy beach can be subject to the effect of wave, ocean current, the nutrition agent of applying is not probably made full use of by petroleum hydrocarbon degradation bacterium and is just lost in ocean environment, brings the threat of eutrophication; Original inhabitants' petroleum hydrocarbon degradation bacterium lacks the enzyme system acting on difficult degradation petroleum pollution, low to the clearance of difficult degradation petroleum pollution; The efficient degrading bacteria added to littoral zone is also faced with the problem that microbial inoculum runs off, and therefore above-mentioned several mode all exists limitation.Therefore, seek a kind of biological restoration device of petroleum pollution sandy beach, by installing biological restoration device on the beach body at petroleum pollution sandy beach, and in device, add immobilization highly effective petroleum surfactant hydrocarbon degradation bacteria, be aided with slow release type nutrition agent simultaneously, make petroleum hydrocarbon degradation bacterium fully contact with the petroleum pollution on sandy beach and it to be removed fast, reach the effect that the fast quick-recovery in petroleum pollution sandy beach is clean.
Summary of the invention:
The object of the invention is to the shortcoming overcoming prior art existence, seek design and a kind of petroleum pollution sandy beach biological restoration device is provided, petroleum pollution sandy beach can be made within the short period (30 ~ 60 days) to obtain fast quick-recovery by this device to clean, complete biological restoration process.
To achieve these goals, agent structure of the present invention comprises upper strata porous PVC back up pad, lower floor's porous PVC back up pad, upper strata silk screen, lower floor's silk screen, plastic frame, plastic layer, Solid nutritional agent and particulate state microbial inoculum; Upper strata porous PVC back up pad is rectangle bulge-structure, and lower floor's porous PVC back up pad is slab construction, and the aperture of upper strata porous PVC back up pad and lower floor's porous PVC back up pad is 10-15mm; Upper strata silk screen is built-in with Solid nutritional agent and particulate state microbial inoculum; The upper strata silk screen adopting stainless steel metal material to make and the aperture of lower floor's silk screen are 2mm, upper strata silk screen is bulge-structure, lower floor's silk screen is two dimensional structure, after lower floor's silk screen covers upper strata silk screen, upside down is between upper strata porous PVC back up pad and lower floor's porous PVC back up pad, and plastic layer is put into plastic frame after upper strata porous PVC back up pad and lower floor's porous PVC back up pad being compressed and formed petroleum pollution sandy beach biological restoration device.
The detailed process that the present invention adopts petroleum pollution sandy beach biological restoration device to realize biological restoration comprises the following steps:
(1), determine the addition of particulate state microbial inoculum and Solid nutritional agent to adopt immobilized particle diameter to be greater than the highly effective petroleum alkane degradation microbial inoculum of 2mm as particulate state microbial inoculum, its addition is determined according to the operation instruction of microbial inoculum; Adopt commercially available slowly-releasing agriculture fertilizer as Solid nutritional agent, the nitrogenous fertilizer added in Solid nutritional agent, phosphate fertilizer amount make the mass ratio of C:N:P be 100:10:1, and wherein C pollutes total C amount that sandy beach is contained in petroleum hydrocarbon; Or adopt NaNO
3, KH
2pO
4prepare voluntarily according to the quality of above-mentioned C:N:P, and utilize prior art to obtain Solid nutritional agent after granulation, slowly-releasing process;
(2), on petroleum pollution sandy beach, contaminated area and the degree of depth is determined, according to the height of pollution depth determination biological restoration device, then using 2 times of length values as biological restoration device of biological restoration device height, the area of single biological restoration device is determined;
(3), by Solid nutritional agent and the particulate state microbial inoculum high spot loading the upper strata silk screen of biological restoration device after gauze parcel, and be inverted in the middle of upper strata porous PVC back up pad and lower floor's porous PVC back up pad after covering lower floor's silk screen, with plastic layer, upper strata porous PVC back up pad is compressed, then load in plastic frame fixing;
(4), according to the Solid nutritional agent in the Solid nutritional agent needing in step (1) to add and particulate state microbial inoculum total amount and each biological restoration device and particulate state microbial inoculum addition, the biological restoration device number needed is determined;
(5), contaminated tideland is divided into latticed,, spaced principle staggered according to biological restoration device, a lot of groove is vertically dug out in contaminated tideland at petroleum pollution sandy beach, the degree of depth of groove is 1.1-1.5 times of biological prosthetic device height, then biological restoration device is imbedded tideland;
(6), determine within 15 days, to be a cycle of operation, within the cycle of operation, the quantity of total petroleum hydrocarbon and petroleum hydrocarbon degradation bacterium in the interstitial water of sandy beach is measured respectively every 3 days, after running one-period, according to the number change situation of total petroleum hydrocarbon and petroleum hydrocarbon degradation bacterium, the microbial inoculum in adjustment or supplementary biological restoration device and Solid nutritional dosage;
(7), 2-4 all after dates, stop biological restoration process, then biological restoration device taken out recycling, realize the biological restoration at petroleum pollution sandy beach.
Compared with prior art, its structure is simple, easy to operate in the present invention, and principle is reliable, and repair rate is high, environmental friendliness, and cost is low, for the biological restoration of petroleum pollution sandy beach or coastal tidal.
Accompanying drawing illustrates:
Fig. 1 is agent structure principle schematic of the present invention.
Fig. 2 is plan structure principle schematic of the present invention.
Fig. 3 is during the embodiment of the present invention adopts the test group of biological restoration device and do not carry out the control group that processes, the number change situation of interstitial water Petroleum Hydrocarbon concentration (a) and petroleum hydrocarbon degradation bacterium (b).
Embodiment:
Below by embodiment, also the invention will be further described by reference to the accompanying drawings.
The agent structure of petroleum pollution sandy beach biological restoration device described in the present embodiment comprises upper strata porous PVC back up pad 1, lower floor's porous PVC back up pad 2, upper strata silk screen 3, lower floor's silk screen 4, plastic frame 5, plastic layer 6, Solid nutritional agent 7 and particulate state microbial inoculum 8; Upper strata porous PVC back up pad 1 is rectangle bulge-structure, and lower floor's porous PVC back up pad 2 is slab construction, and the aperture of upper strata porous PVC back up pad 1 and lower floor's porous PVC back up pad 2 is 10-15mm; Solid nutritional agent 7 and particulate state microbial inoculum 8 in upper strata silk screen 3; The upper strata silk screen 3 adopting stainless steel metal material to make and the aperture of lower floor's silk screen 4 are 2mm, upper strata silk screen 3 is bulge-structure, lower floor's silk screen 4 is two dimensional structure, after lower floor's silk screen 4 covers upper strata silk screen 3, upside down is between upper strata porous PVC back up pad 1 and lower floor's porous PVC back up pad 2, and plastic layer 6 is put into plastic frame 5 after upper strata porous PVC back up pad 1 and lower floor's porous PVC back up pad 2 being compressed and formed petroleum pollution sandy beach biological restoration device.
The present embodiment realizes, to the biological restoration at petroleum pollution sandy beach, specifically comprising the following steps in the biological restoration device of petroleum pollution sandy beach:
(1), determine the addition of particulate state microbial inoculum 8 and Solid nutritional agent 7 to adopt immobilized particle diameter to be greater than the highly effective petroleum alkane degradation microbial inoculum of 2mm as particulate state microbial inoculum 8, its addition is determined according to the operation instruction of highly effective petroleum alkane degradation microbial inoculum; Adopt commercially available slowly-releasing agriculture fertile in Solid nutritional agent 7, the nitrogenous fertilizer added in Solid nutritional agent 7, phosphate fertilizer amount make the mass ratio of C:N:P be 100:10:1, and wherein C pollutes total C amount that sandy beach is contained in petroleum hydrocarbon; Or adopt NaNO
3, KH
2pO
4prepare voluntarily according to the quality of above-mentioned C:N:P, and utilize prior art to obtain Solid nutritional agent 7 after granulation, slowly-releasing process;
(2), on petroleum pollution sandy beach, contaminated area and the degree of depth is determined, according to the height of pollution depth determination biological restoration device, then using 2 times of length values as biological restoration device of biological restoration device height, the area of single biological restoration device is determined;
(3), by Solid nutritional agent 7 and particulate state microbial inoculum 8 high spot loading the upper strata silk screen 3 of biological restoration device after gauze parcel, and be inverted in the middle of upper strata porous PVC back up pad 1 and lower floor's porous PVC back up pad 2 after covering lower floor's silk screen 4, with plastic layer 6, upper strata porous PVC back up pad 1 is compressed, then load in plastic frame 5 fixing;
(4), according to the Solid nutritional agent 7 in the Solid nutritional agent 7 needing in step (1) to add and particulate state microbial inoculum 8 total amount and each biological restoration device and particulate state microbial inoculum 8 addition, the biological restoration device number needed is determined;
(5), contaminated tideland is divided into latticed,, spaced principle staggered according to biological restoration device, a lot of groove is vertically dug out in contaminated tideland at petroleum pollution sandy beach, the degree of depth of groove is 1.1-1.5 times of biological prosthetic device height, then biological restoration device is imbedded tideland;
(6), determine within 15 days, to be a cycle of operation, within the cycle of operation, the quantity of total petroleum hydrocarbon and petroleum hydrocarbon degradation bacterium in the interstitial water of sandy beach is measured respectively every 3 days, after running one-period, according to the number change situation of total petroleum hydrocarbon and petroleum hydrocarbon degradation bacterium, the microbial inoculum in adjustment or supplementary biological restoration device and the amount of Solid nutritional agent;
(7), 2-4 all after dates, stop biological restoration process, then biological restoration device taken out recycling, realize the biological restoration at petroleum pollution sandy beach.
Embodiment 1:
The present embodiment has carried out simultaneous test on petroleum pollution sandy beach, sandy beach is divided into 2 blocks, is respectively test group and control group.Control group does not do any process, and test group adopts biological restoration device to process, and first calculates N, P nutrition of needs according to the petroleum hydrocarbon C content on sandy beach, prepares slow release type N, P nutrition agent; Adopt immobilization highly effective petroleum surfactant hydrocarbon degradation bacteria as microbial inoculum, according to the usefulness determination applied amount of microbial inoculum, then nutrition agent and microbial inoculum are added in biological restoration device, the Polluted area at sandy beach is divided into latticed, by biological restoration device interval, be staggered; 30d is carried out in test, total petroleum hydrocarbon concentration in the interstitial water of 3d mensuration sandy beach and the quantity of petroleum hydrocarbon degradation bacterium, as shown in Figure 3, as can be seen from the figure, after adopting biological restoration device, total petroleum hydrocarbon concentration in interstitial water is starkly lower than control group, and the quantity of petroleum hydrocarbon degradation bacterium is apparently higher than the control group not adding process.
Claims (2)
1. the biological restoration device at petroleum pollution sandy beach, is characterized in that agent structure comprises upper strata porous PVC back up pad, lower floor's porous PVC back up pad, upper strata silk screen, lower floor's silk screen, plastic frame, plastic layer, Solid nutritional agent and particulate state microbial inoculum; Upper strata porous PVC back up pad is rectangle bulge-structure, and lower floor's porous PVC back up pad is slab construction, and the aperture of upper strata porous PVC back up pad and lower floor's porous PVC back up pad is 10-15mm; Upper strata silk screen is built-in with Solid nutritional agent and particulate state microbial inoculum; The upper strata silk screen adopting stainless steel metal material to make and the aperture of lower floor's silk screen are 2mm, upper strata silk screen is bulge-structure, lower floor's silk screen is two dimensional structure, after lower floor's silk screen covers upper strata silk screen, upside down is between upper strata porous PVC back up pad and lower floor's porous PVC back up pad, and plastic layer is put into plastic frame after upper strata porous PVC back up pad and lower floor's porous PVC back up pad being compressed and formed petroleum pollution sandy beach biological restoration device.
2. the biological restoration device at petroleum pollution sandy beach according to claim 1, is characterized in that adopting this device to the detailed process that biological restoration is carried out at petroleum pollution sandy beach be:
(1), determine the addition of particulate state microbial inoculum and Solid nutritional agent to adopt immobilized particle diameter to be greater than the highly effective petroleum alkane degradation microbial inoculum of 2mm as particulate state microbial inoculum, its addition is determined according to the operation instruction of microbial inoculum; Adopt commercially available slowly-releasing agriculture fertilizer as Solid nutritional agent, the nitrogenous fertilizer added in Solid nutritional agent, phosphate fertilizer amount make the mass ratio of C:N:P be 100:10:1, and wherein C pollutes total C amount that sandy beach is contained in petroleum hydrocarbon; Or adopt NaNO
3, KH
2pO
4prepare voluntarily according to the quality of above-mentioned C:N:P, and utilize prior art to obtain Solid nutritional agent after granulation, slowly-releasing process;
(2), on petroleum pollution sandy beach, contaminated area and the degree of depth is determined, according to the height of pollution depth determination biological restoration device, then using 2 times of length values as biological restoration device of biological restoration device height, the area of single biological restoration device is determined;
(3), by Solid nutritional agent and the particulate state microbial inoculum high spot loading the upper strata silk screen of biological restoration device after gauze parcel, and be inverted in the middle of upper strata porous PVC back up pad and lower floor's porous PVC back up pad after covering lower floor's silk screen, with plastic layer, upper strata porous PVC back up pad is compressed, then load in plastic frame fixing;
(4), according to the Solid nutritional agent in the Solid nutritional agent needing in step (1) to add and particulate state microbial inoculum total amount and each biological restoration device and particulate state microbial inoculum addition, the biological restoration device number needed is determined;
(5), contaminated tideland is divided into latticed,, spaced principle staggered according to biological restoration device, a lot of groove is vertically dug out in contaminated tideland at petroleum pollution sandy beach, the degree of depth of groove is 1.1-1.5 times of biological prosthetic device height, then biological restoration device is imbedded tideland;
(6), determine within 15 days, to be a cycle of operation, within the cycle of operation, the quantity of total petroleum hydrocarbon and petroleum hydrocarbon degradation bacterium in the interstitial water of sandy beach is measured respectively every 3 days, after running one-period, according to the number change situation of total petroleum hydrocarbon and petroleum hydrocarbon degradation bacterium, the microbial inoculum in adjustment or supplementary biological restoration device and Solid nutritional dosage;
(7), 2-4 all after dates, stop biological restoration process, then biological restoration device taken out recycling, realize the biological restoration at petroleum pollution sandy beach.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510455134.7A CN105036354B (en) | 2015-07-30 | 2015-07-30 | Bioremediation device of petroleum-polluted sand beach |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510455134.7A CN105036354B (en) | 2015-07-30 | 2015-07-30 | Bioremediation device of petroleum-polluted sand beach |
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| Publication Number | Publication Date |
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| CN105036354A true CN105036354A (en) | 2015-11-11 |
| CN105036354B CN105036354B (en) | 2016-11-30 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002205092A (en) * | 2001-01-15 | 2002-07-23 | Ryoichi Tsumoto | Plantation for water cleaning |
| RU2008123891A (en) * | 2008-06-18 | 2009-12-27 | Екатерина Анатольевна Сребняк (RU) | CARTRIDGE FOR CLEANING NATURAL WATERS FROM OIL CONTAMINATIONS |
| CN102021132A (en) * | 2009-12-03 | 2011-04-20 | 北京航空航天大学 | Method for screening and remediation of petroleum-contaminated soil bioremediation agent |
| CN102249428A (en) * | 2011-06-10 | 2011-11-23 | 吉林大学 | In-situ remediation method of petroleum-polluted groundwater |
| US20110315628A1 (en) * | 2010-06-24 | 2011-12-29 | Shaw Mark D | System and method for capturing and bioremediating hydrocarbon pollutants using dynamic filters |
| CN102491494A (en) * | 2011-11-24 | 2012-06-13 | 北京师范大学 | Simulator for adsorption and strengthened microbiological degradation of marine spilled oil pollution |
-
2015
- 2015-07-30 CN CN201510455134.7A patent/CN105036354B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002205092A (en) * | 2001-01-15 | 2002-07-23 | Ryoichi Tsumoto | Plantation for water cleaning |
| RU2008123891A (en) * | 2008-06-18 | 2009-12-27 | Екатерина Анатольевна Сребняк (RU) | CARTRIDGE FOR CLEANING NATURAL WATERS FROM OIL CONTAMINATIONS |
| CN102021132A (en) * | 2009-12-03 | 2011-04-20 | 北京航空航天大学 | Method for screening and remediation of petroleum-contaminated soil bioremediation agent |
| US20110315628A1 (en) * | 2010-06-24 | 2011-12-29 | Shaw Mark D | System and method for capturing and bioremediating hydrocarbon pollutants using dynamic filters |
| CN102249428A (en) * | 2011-06-10 | 2011-11-23 | 吉林大学 | In-situ remediation method of petroleum-polluted groundwater |
| CN102491494A (en) * | 2011-11-24 | 2012-06-13 | 北京师范大学 | Simulator for adsorption and strengthened microbiological degradation of marine spilled oil pollution |
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| CN105036354B (en) | 2016-11-30 |
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