CN105028490B - A method of utilizing the leaf of lantana or the flower poisoning adult Pomacea canaliculata of the leaf of Allemanda cathartica L or Allemanda cathartica L - Google Patents
A method of utilizing the leaf of lantana or the flower poisoning adult Pomacea canaliculata of the leaf of Allemanda cathartica L or Allemanda cathartica L Download PDFInfo
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Abstract
The invention discloses a kind of methods using the leaf of lantana or the flower poisoning adult Pomacea canaliculata of the leaf of Allemanda cathartica L or Allemanda cathartica L, spend three kinds of plant organs as material using the leaf of lantana or the leaf of Allemanda cathartica L or Allemanda cathartica L, crushed, being sieved is made pulvis of different sizes, be arranged to clear water control, 1g/L, 3g/L, 6g/L, 9g/L, 12g/L, 15g/L, 18g/L, 21g/L concentration and indoor poisoning carried out at spiral shell to Pomacea canaliculata；Lantana and switch Huang the moon all have molluscicidal effect, and the leaf molluscicidal effect of lantana is more preferable than Allemanda cathartica L flower, leaf, under same concentrations, the time of poisoning is longer, and effect is better, when poisoning, powder particles size also has a certain impact, and mesh number is bigger, and toxic effect is better.
Description
Technical field
The present invention relates to a kind of method for killing adult Pomacea canaliculata, especially a kind of leaves or Allemanda cathartica L using lantana
Leaf or Allemanda cathartica L flower poisoning adult Pomacea canaliculata method.
Background technique
Pomacea canaliculata also known as Ampullaria gigas, apple spiral shell, amphibious freshwater shellfish software is biological, and Mollusca, abdominal foot are belonged in classification
Guiding principle, Mesogastropoda, Ampullariidae, bottle spiral shell category, belong to Perenniporia martius kind；South America Amazon River basin is originated in, in 20 generation
Record the eighties after Pomacea canaliculata be gradually introduced as a kind of food to countries such as Japan, Philippine, Vietnam, Thailand；1981, good fortune
Longevity spiral shell is similarly firstly introduced into China Guangdong as a kind of food, after 1984, is widely cultivated in Guangdong, and rapidly diffuse into
The ground such as Guangxi, Fujian, Hainan, Zhejiang, Shanghai, Jiangsu, after because of Pomacea canaliculata food flavor Yu Tianye that is bad, and being abandoned；Due to blindly
It introduces and manages improperly, Pomacea canaliculata cause disaster in south China by many provinces outbursts at present, and becomes China and other Asia states
The pernicious aquatic animal of family's hazard rice and other water plants, is known as " rice killer "；Pomacea canaliculata adaptability is strong, likes
It is pure and fresh in water quality, it lives in the fresh water of foodstuff abundance, multigroup collection inhabits phytal zone, or is adsorbed on water plant cauline leaf；It is suitable
It is preferably grown under conditions of water temperature is 25~32 DEG C, water temperature is more than that 35 DEG C of growths are decreased obviously, and the existence critical water temperature of highest is 45
DEG C, minimum critical water temperature is 5 DEG C；Pomacea canaliculata is the omnivorousness snail based on vegetal bait, and feeding habits are wide, staple food rice, hay
The aquatic crops such as white, duckweed, the sweetish food of preference；Although it is water biological species, water body life of short duration can be left, it can be with
It survives 6~8 months in wet soil, once water layer is restored in field, and energy activity feeding is caused harm；It breeds within Pomacea canaliculata 1 year
2~3 generations, and have apparent generation overlap phenomenon, with young spiral shell or at spiral shell 2~3 under aquatic crops base portion or paddy field soil table
The depths ㎝ is overwintering on field side, limes marginis etc.；Pieces of an egg are often produced in the table for planting item or drying objects from 10~15 ㎝ or more of the water surface
Face, such as rice, wild rice stem, barnacle furrow bank, ridge, weeds；1 female spiral shell 1 year total can produce hatches young spiral shell more than 32.5 ten thousand only,
The feeding habits of the extremely strong Pomacea canaliculata of reproductive capacity are very miscellaneous, and almost any biology in feeding environment, also attacks other snails and spiral shell ovum sometimes,
But its Major Foods or higher plant and some dryland vegetables etc..
As Pomacea canaliculata is in the increase of China's invasion time, in addition global climate warms, Pomacea canaliculata annidation
Constantly enhancing and artificial introducing, endangering area gradually will migrate and extend to the northern area of China；Pomacea canaliculata is suitable
In China, wide geographic area lives harm, endangers area's area and accounts for 60% or so, and the Rice Production and ecological safety to China constitute serious
It threatens；Meanwhile in recent years, due to the variation of agricultural planting structure, the type that Pomacea canaliculata harms the crops also is increasing, except harm
Outside rice, Pomacea canaliculata also encroaches on thatch reality, water chestnut, sweet potato, arrowhead, Chinese milk vetch and aquatic vegetable in dark and damp habitat, in recent years,
Pomacea canaliculata is even more widespread harm, causes extensive concern.
Currently, there are many modes for Pomacea canaliculata prevention and treatment, such as: the finishing of winter rice field, Artificially taking away the egg cases, is tended ducks at science Guan Shui
Spiral shell etc. is eaten, but because being restricted by the stability of effect, the selection of action opportunity and the factors such as the acquisition of material and cost, mostly not
It is preferably a wide range of to use.Pesticide control is one of the measure of Pomacea canaliculata prevention and treatment, and used medicament is substantially chemical agent, in addition to
Environment is caused outside a degree of pollution, it is higher there is also price the problems such as；The chemicals for being sprayed at vegetable surface can be because
The health of eater is influenced, residue is easy to endanger ecological environment, and the Pomacea canaliculata in some areas generates chemical pesticide
Resistance, makes difficulty of prevention and cure and added cost.
The natural drug of plant origin, especially with discarded in the local plant with affluent resources or processing of farm products
Object is as natural drug, with the incomparable advantage of chemicals: abundance, low in cost, easy drop in the natural environment
Solution, small pollution of the environment.
Summary of the invention
The object of the present invention is to provide a kind of flowers using the leaf of lantana or the leaf of Allemanda cathartica L or Allemanda cathartica L to kill
The method of adult Pomacea canaliculata.
This method using the leaf of lantana or the flower poisoning adult Pomacea canaliculata of the leaf of Allemanda cathartica L or Allemanda cathartica L:
It spends two kinds of plant organs as material using the leaf or Allemanda cathartica L of Allemanda cathartica L, is crushed, being sieved is made
Then pulvis of different sizes spends chlorine water or tap water and stands 48h, be configured to 6g/L, 9g/L, 12g/L, 15g/L,
18g/L, 21g/L concentration carry out indoor poisoning at spiral shell to Pomacea canaliculata；
The pulvis is spent and two kinds of plant organ samples of Allemanda cathartica L leaf, difference the preparation method comprises the following steps: acquiring Allemanda cathartica L
It is cleaned with clear water, after draining, using electric heating constant-temperature blowing drying box, is dried under 60 DEG C of constant temperature for 24 hours, then,
Taking-up is crushed with high speed Universal pulverizer, is then screened into different mesh with the mesh screen of 20 mesh, 30 mesh, 40 mesh, 50 mesh, 60 mesh respectively
Several powder, it is spare to be placed in sealed bag.
Advantageous effect of the invention:
Use material for the natural drug of plant origin, with the incomparable advantage of chemicals: abundance, cost
Cheap, degradable, small pollution of the environment in the natural environment.
The present invention has carried out the screening test of plant source molluscacide, and lantana and switch Huang the moon all have molluscicidal effect,
The leaf molluscicidal effect of lantana is more preferable than Allemanda cathartica L flower, leaf, and under same concentrations, the time of poisoning is longer, and effect is better, poison
When killing, powder particles size also has a certain impact, and mesh number is bigger, and toxic effect is better.Further to utilize plant abundant
Resource provides basic data for Pomacea canaliculata study on prevention, while also providing material for bionical pharmaceutical synthesis.
(1) vegetable material lantana, the frequently seen plants that Allemanda cathartica L is South China, obtain and are easy.
(2) processing method is easy to operate, and without complicated program, convenient for operating in field, save the cost reduces pollution.
(3) be conducive to control invasion animal Pomacea canaliculata, and open new approach for the resource utilization of plant, for
The research of invasion ecology and biotic intrusion control technology has great reference value.
(4) have the characteristics that environmental pollution effect is relatively small；Labor intensity is low, has wide range of applications, and work efficiency is high.
Specific embodiment
Embodiment:
Utilize the method for the leaf of lantana or the flower poisoning adult Pomacea canaliculata of the leaf of Allemanda cathartica L or Allemanda cathartica L:
Spend three kinds of plant organs as material using the leaf of lantana or the leaf of Allemanda cathartica L or Allemanda cathartica L, by its powder
It is broken, sieving pulvis of different sizes is made, be arranged to clear water control, 1g/L, 3g/L, 6g/L, 9g/L, 12g/L, 15g/L,
18g/L, 21g/L concentration carry out indoor poisoning at spiral shell to Pomacea canaliculata.Lantana, Allemanda cathartica L all have certain molluscicidal effect.
In each organ, the molluscicidal effect of lantana leaf is best, followed by the flower of Allemanda cathartica L, after each pulvis poisoning 72h of 60 mesh, Ma Ying
The half lethal concentration of red leaf is 6.532g/L, and the half lethal concentration of Allemanda cathartica L flower is 11.853g/L, half cause of Allemanda cathartica L leaf
Dead concentration is 12.805g/L.Under same concentrations, the time of poisoning is longer, and effect is better.When poisoning, each organ powder particles
Size also will affect ovicidal trait, and mesh number is bigger, and toxic effect is better.
Specifically comprise the steps of:
1.1 materials:
1.1.1 experimental plant: lantana (Lantana camara), Allemanda cathartica L (Allamanda
cathartica)。
1.1.2 Pomacea canaliculata: picking up from paddy field at spiral shell, and spiral shell is 4 more than the monthly age, quality 20g~25g, and individual is big, spiral shell
The individual that shell is intact, healthy.Laboratory rearing is taken back after acquisition.
1.1.3 instrument and equipment includes:
Electric heating constant-temperature blowing drying box, high speed Universal pulverizer, 20 mesh filter screen, 30 mesh filter screen, 40 mesh filter screen, 50
Mesh filter screen, 60 mesh filter screen, clip, plastic tub, cover drum, screen cloth, glass bar, 500mL beaker, disposal plastic cup,
Disposable glove, sealed bag, electronic analytical balance, refrigerator.
1.2 experimental methods:
1.2.1 the preparation of plant sample:
Lantana leaf, Allemanda cathartica L flower and three kinds of plant organ samples of Allemanda cathartica L leaf are acquired, are washed respectively with clear water
Only, it after draining, is dried for 24 hours, is then taken out with the omnipotent powder of high speed under 60 DEG C of constant temperature with electric heating constant-temperature blowing drying box
Broken machine crushes, and is then screened into the powder of different meshes with the mesh screen of 20 mesh, 30 mesh, 40 mesh, 50 mesh, 60 mesh respectively, is placed in envelope
Pocket is spare.
1.2.2 Pomacea canaliculata is raised and train
It is raised and train next being placed in 5 drums at spiral shell will be acquired, drainage was added or tap water stands 48h, the depth of water
Simultaneously suitable feed is added in 20cm, 24 hours oxygenations, this experiment feed used is fresh romaine lettuce.
To keep water quality pure and fresh, a water should be replaced daily, feeds enough feeds sooner or later daily.Nurturing period ensures water
Matter is pure and fresh and environment quiet.
1.2.3 spiral shell experiment is killed in the interior of plant powder:
Then the amount of plant sample needed for weighing various gradient concentrations on electronic analytical balance spends chlorine water or tap water
It stands 48 h and is configured to required concentration.Be each configured to clear water control 1g/L, 3g/L, 6g/L, 9g/L, 12g/L, 15g/L,
4 groups of repetitions are arranged in the medical fluid of 18g/L, 21g/L totally 9 kinds of gradient concentrations, each concentration, every group of placement 20 only at spiral shell, carry out for 24 hours,
The death conditions of 48h, 72h, 96h different periods is observed, and no longer feeding feed in experimentation in time takes dead individual
Out, the number of record different periods Pomacea canaliculata death in time, is made table.
1.2.4 Pomacea canaliculata life or death standard of perfection:
After medicine for 24 hours, 48h, 72h, 96h take out the dead spiral shell of suspection in each processing, put in a suitable place to breed observed in going chlorine water respectively, for 24 hours
It is interior cannot open operculum it is movable for death.
2 interpretations of result:
Spiral shell test is killed in the leaf powder solvent compartment of 2.1 lantanas:
Table 1~5 is that the lantana leaf powder being sieved with the mesh screen of 20 mesh, 30 mesh, 40 mesh, 50 mesh, 60 mesh is processed into respectively
Year Pomacea canaliculata is provided with clear water and compares 1g/L, 3g/L, 6g/L, 9g/L, 12g/L, 15g/L, 18g/L, 21g/L concentration ladder.By
Analysis can obtain in table, 20 mesh lantanas under 1g/L concentration, for 24 hours, the average killing snail rate of 48h, 72h be respectively 2.50%, 5.50%,
6.25%.Under 18g/L concentration, for 24 hours, after 48h, 72h, killing snail rate 30%, 78.75%, 90%.Show that lantana just has in 1g/L
There is ovicidal trait, under 18g/L concentration after 96h, toxic effect reaches 100%.60 mesh lantanas under 18g/L concentration, for 24 hours,
The average killing snail rate of 48h, 72h are respectively 36.25%, 82.50%, 93.75%.Show that lantana has ovicidal trait in 1g/L,
72h reaches 79.5%, 96h and then reaches 100% under 18g/L concentration.
Spiral shell test is killed in the powder solvent compartment of 2.2 Allemanda cathartica Ls flower:
Table 6~10 is to handle adult Fu Shou respectively with the Allemanda cathartica L pollen end of 20 mesh, 30 mesh, 40 mesh, 50 mesh, 60 mesh
Spiral shell is provided with clear water control, 1g/L, 3g/L, 6g/L, 9g/L, 12g/L, 15g/L, 18g/L, 21g/L concentration ladder.By in table
Analysis obtains, 20 mesh Allemanda cathartica Ls flower under 1g/L concentration, for 24 hours, the average killing snail rate of 48h, 72h be respectively 1.25%, 1.25%,
2.50%.Under 18g/L concentration, for 24 hours, after 48h, 72h, killing snail rate 29.50%, 56.25%, 72.50%.60 mesh 1g/L switch are yellow
Cicada fungus solution, poisoning for 24 hours, after 48h, 72h, killing snail rate 0%, 1.25%, 1.25%.Show that Allemanda cathartica L flower does not have in 1g/L
There is ovicidal trait, and 72h killing snail rate reaches 86.25%, 96h and then also reaches 100% under 21g/L concentration.
Spiral shell test is killed in the powder solvent compartment of 2.3 Allemanda cathartica L leaves:
Table 11~15 is to handle adult Fu Shou respectively with the Allemanda cathartica L leaf powder of 20 mesh, 30 mesh, 40 mesh, 50 mesh, 60 mesh
Spiral shell is provided with clear water control, 1g/L, 3g/L, 6g/L, 9g/L, 12g/L, 15g/L, 18g/L, 21g/L isoconcentration ladder.By table
In analyze, 20 mesh Allemanda cathartica L leaves under 1g/L concentration, for 24 hours, the average killing snail rate of 48h, 72h be respectively 1.25%, 2.50%,
2.50%.Under 3g/L concentration, for 24 hours, after 48h, 72h, killing snail rate is also unobvious, respectively 1.25%, 2.50%, 3.75%.60
Under mesh 21g/L concentration, for 24 hours, after 48h, 72h, after the death rate 32.50%, 62.50%, 83.75%, 96h, killing snail rate is up to 100%.
The half lethal concentration of 2.4 lantanas and Allemanda cathartica L powder solvent poisoning Pomacea canaliculata:
Table 16 is 20 mesh, 30 mesh, 40 mesh, 50 mesh, the lantana of 60 mesh and Allemanda cathartica L leaf powder-processed adult good fortune respectively
The half lethal concentration obtained after longevity spiral shell, under the conditions of for 24 hours, 20 mesh, 30 mesh, 40 mesh, 50 mesh, the semilethal of 60 mesh lantana leaves are dense
Degree is 39.691g/L, 35.284g/L, 31.824g/L, 28.321g/L, 27.688g/L, is sequentially reduced, similarly 48h, 72h item
Under part, also there is this rule.Thus, mesh number be on half lethal concentration it is influential, mesh number is bigger, and half lethal concentration is smaller.
Under the conditions of 72h, the half lethal concentration of the lantana leaves of 20 mesh, Allemanda cathartica L flower and leaf is respectively 7.633g/L, 12.790g/
L, 15.308g/L is sequentially increased, this rule is similarly also complied with for 24 hours, under 48h and other sieve meshes, thus, three kinds of plants
The descending half lethal concentration of organ is Allemanda cathartica L leaf, Allemanda cathartica L flower, Leaves of Lantana Camara respectively.
3, it summarizes:
The death rate all very littles of spiral shell after clear water control group each time, or even be often zero, it will thus be seen that starvation is not to lead
Cause into the principal element of spiral shell death.In this experiment, hungry lethal factor can be ignored.
The toxic action of this experimental study lantana and Allemanda cathartica L to adult Pomacea canaliculata.Experimental data shows: horse
Cherry is red, Allemanda cathartica L has certain molluscicidal effect.The molluscicidal effect of lantana leaf is best, followed by the flower of Allemanda cathartica L, such as
Under the conditions of 72h, the half lethal concentration of the lantana leaves of 60 mesh, Allemanda cathartica L flower and leaf is respectively 6.532g/L, 11.853g/
L, 12.805g/L is successively increased, and has identical rule with the lower three kinds of organs of sieve mesh at other times.When poisoning, powder particles are big
Small also to will affect ovicidal trait, mesh number is bigger, and particle is smaller, and toxic effect is better, such as under the conditions of for 24 hours, 20 mesh, 30 mesh, 40
Mesh, 50 mesh, 60 mesh lantana leaves half lethal concentration be 39.691g/L, 35.284g/L, 31.824g/L, 28.321g/L,
27.688g/L is successively reduced, while at other times, also has identical rule for other organs.Different Organs with concentration increasing
Add, ovicidal trait also increases.Under same concentrations, the time of poisoning is longer, and effect is better.
Table 1~5:
Table 1 crosses average mortality (%) of the lantana leaf powder to Pomacea canaliculata of 20 meshes
Note: the significant difference in the level of p=0.05 is indicated with the letter marked after column data in table, there is same word
Matrix shows that difference is not significant, the same below
Table 2 crosses average mortality (%) of the lantana leaf powder to Pomacea canaliculata of 30 meshes
Table 3 crosses average mortality (%) of the lantana leaf powder to Pomacea canaliculata of 40 meshes
Table 4 crosses average mortality (%) of the lantana leaf powder to Pomacea canaliculata of 50 meshes
Table 5 crosses average mortality (%) of the lantana leaf powder to Pomacea canaliculata of 60 meshes
Table 6~10:
Table 6 crosses the average mortality (%) of the Allemanda cathartica L pollen foot couple Pomacea canaliculata of 20 meshes
Table 7 crosses the average mortality (%) of the Allemanda cathartica L pollen foot couple Pomacea canaliculata of 30 meshes
Table 8 crosses the average mortality (%) of the Allemanda cathartica L pollen foot couple Pomacea canaliculata of 40 meshes
Table 9 crosses the average mortality (%) of the Allemanda cathartica L pollen foot couple Pomacea canaliculata of 50 meshes
Table 10 crosses the average mortality (%) of the Allemanda cathartica L pollen foot couple Pomacea canaliculata of 60 meshes
Table 11~15:
Table 11 crosses average mortality (%) of the Allemanda cathartica L leaf powder to Pomacea canaliculata of 20 meshes
Table 12 crosses average mortality (%) of the Allemanda cathartica L leaf powder to Pomacea canaliculata of 30 meshes
Table 13 crosses average mortality (%) of the Allemanda cathartica L leaf powder to Pomacea canaliculata of 40 meshes
Table 14 crosses average mortality (%) of the Allemanda cathartica L leaf powder to Pomacea canaliculata of 50 meshes
Table 15 crosses average mortality (%) of the Allemanda cathartica L leaf powder to Pomacea canaliculata of 60 meshes
Table 16:
The half lethal concentration (g/L) of the powder solvent poisoning Pomacea canaliculata of 16 3 kinds of plant organs of table
Claims (1)
1. a kind of method of the pulvis poisoning adult Pomacea canaliculata of flower of the leaf or Allemanda cathartica L using Allemanda cathartica L, feature exist
In:
It spends two kinds of plant organs as material using the leaf or Allemanda cathartica L of Allemanda cathartica L, is crushed, being sieved is made size
Then the pulvis of different size spends chlorine water or tap water and stands 48h, be configured to 6g/L, 9g/L, 12g/L, 15g/L, 18g/L,
21g/L concentration carries out indoor poisoning at spiral shell to Pomacea canaliculata；
The pulvis the preparation method comprises the following steps: acquisition Allemanda cathartica L is colored and two kinds of plant organ samples of Allemanda cathartica L leaf, respectively with clear
Water is cleaned, and after draining, using electric heating constant-temperature blowing drying box, is dried under 60 DEG C of constant temperature for 24 hours, then, is taken out
It is crushed with high speed Universal pulverizer, is then screened into different meshes with the mesh screen of 20 mesh, 30 mesh, 40 mesh, 50 mesh, 60 mesh respectively
It is spare to be placed in sealed bag for powder.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006036745A (en) * | 2004-07-26 | 2006-02-09 | Yoshihiko Kamimura | Freshwater snail exterminator |
| CN103004891A (en) * | 2012-12-10 | 2013-04-03 | 华南农业大学 | Application of alien invasive plant Ageratum conyzoides L. in control of golden apple snail |
| CN103039535A (en) * | 2012-12-10 | 2013-04-17 | 华南农业大学 | Application of alien invasive plant wedelia trilobata in killing ampullaria gigas |
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2015
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006036745A (en) * | 2004-07-26 | 2006-02-09 | Yoshihiko Kamimura | Freshwater snail exterminator |
| CN103004891A (en) * | 2012-12-10 | 2013-04-03 | 华南农业大学 | Application of alien invasive plant Ageratum conyzoides L. in control of golden apple snail |
| CN103039535A (en) * | 2012-12-10 | 2013-04-17 | 华南农业大学 | Application of alien invasive plant wedelia trilobata in killing ampullaria gigas |
Non-Patent Citations (2)
| Title |
|---|
| "Effect of the aqueous extract from Allamanda cathartica L (Apocynaceae) upon Bradybaena similaris (Férussac,1821) (Bradybaenidae) in laboratory conditions";Carla Aparecida Azevedo do Nascimento 等;《Revista Brasileira de Zoociências》;20060430;第8卷(第1期);摘要、第78页右栏倒数第2段、第81页左栏最后一段 |
| "四种与福寿螺（Ampullaria gigas）同源地入侵植物的杀螺效果";曾坤玉 等;《生态学报》;20080115;第28卷(第1期);摘要、第261页第1.2节、第263页第2.3节、表1 |
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