CN105028413A - Cold resistance treating fluid for peanut seeds - Google Patents

Cold resistance treating fluid for peanut seeds Download PDF

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Publication number
CN105028413A
CN105028413A CN201510366619.9A CN201510366619A CN105028413A CN 105028413 A CN105028413 A CN 105028413A CN 201510366619 A CN201510366619 A CN 201510366619A CN 105028413 A CN105028413 A CN 105028413A
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China
Prior art keywords
peanut
peanut seeds
cold resistance
weight
cold
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CN201510366619.9A
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Chinese (zh)
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CN105028413B (en
Inventor
于树涛
于洪波
于国庆
赵立仁
王海新
孙泓希
任亮
尤淑丽
史普想
张宇
蔡立夫
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LIAONING WIND DRIFT SAND MELIORATE APPLICATIONS INSTITUTE
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LIAONING WIND DRIFT SAND MELIORATE APPLICATIONS INSTITUTE
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Publication of CN105028413A publication Critical patent/CN105028413A/en
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Abstract

The invention relates to cold resistance treating fluid for peanut seeds, which comprises the following components: 500 to 1500 parts of by weight of proline, 0.05 to 0.15 parts by weight of abscisic acid, 100000 to 200000 parts by weight of polyethylene glycol-6000 and 1000000 parts by weight of water. According to the cold resistance treating fluid for the peanut seeds provided by the invention, the ratio of the proline and the abscisic acid can improve the freeze resistance capability of seeds, with the addition of the ratio of the polyethylene glycol-6000, the seed germination rate is effectively improved. Early sowing of the peanut seeds can be realized, so that the peanut seeds have the cold resistance, can grown up from the soil after the ground temperature is increased, and sprout rapidly. The cold resistance treating fluid for the peanut seeds can remarkably improve the cold resistance of the peanut seeds, resist reduction of output caused by encountering a cold current and chilling damage after spring sowing, and the rate of emergence is significantly increased.

Description

Peanut seed resists cold treatment fluid
Technical field
The present invention relates to agricultural biological technical field, relate to peanut seed specifically and to resist cold treatment fluid.
Background technology
Peanut is the important oil crop of China and economic crops, but can be subjected to various environment-stress at whole growthdevelopmental stage, and wherein temperature is exactly one of key factor affecting Development of Peanut.Because peanut is comparatively extensive at China's planting range, Heilongjiang Province's Heihe City (north latitude 50 °) has been planted in the north.These high latitude areas usually there will be the microthermal climate of climatic extreme year.After peanut seeding, the normal low temperature that meets with threatens, and the generation physiological performance of this disaster is: poor growth, and the biomass in the plant unit interval is little, and metabolism is weak, and root water uptake reduces, and transpiration weakens, and finally causes the reduction of output.In addition, the Northeast China grey bristle production area being in high latitude area due to breeding time short, to plant Pearl Type and Valencia type peanut, and the plain edition major peanut with high yield potential is often subject to restriction breeding time, thus the lifting of restriction output.Be in the lifting of peasant in order to output of high latitude area in recent years, the major peanut that blind import breeding time is long, after sowing in spring, employings as many in low temperature are reseeded, are ruined kind of a method, time-consumingly take a lot of work, and also add production cost.
At present, treatment fluid research aspect is resisted cold seldom for peanut seed in China high latitude area, and peanut is sowed in advance, avoids the harm of cold in the late spring, is beneficial to prolong growth period, improves peanut yield significant.
Summary of the invention
The object of the invention is the problem for resisting low temperature after peanut seeding, providing a kind of peanut seed to resist cold treatment fluid, peanut cold tolerance can be significantly improved, ensure emergence rate.
For achieving the above object, the technical solution used in the present invention is:
Peanut seed resists cold treatment fluid, includes following component:
The proline of 500-1500 weight portion, the abscisic acid of 0.05-0.15 weight portion, the weight portion PEG-4000 of 100000-200000, the water of 1000000 weight portions.
Described peanut seed resists cold treatment fluid, includes following component:
The proline of 1000 weight portions, the abscisic acid of 0.10 weight portion, the PEG-4000 of 150000 weight portions, the water of 1000000 weight portions.
Described peanut seed resists cold treatment fluid, also includes the calcium chloride of 10-20 weight portion, the boric acid of 1000-5000 weight portion.
Described peanut seed resists cold treatment fluid, also includes the calcium chloride of 15 weight portions, the boric acid of 2500 weight portions.
The advantage that the present invention has and effect are:
Peanut seed of the present invention resists cold treatment fluid owing to having proline, abscisic acid, PEG-4000 and water; the effect of Synergistic can be produced after these four kinds of component mixing; when plant is subject to affecting of multiple adverse environment; proline can be combined more fully with protein under the impact of other composition; diaphragm is formed between protein surface and hydrone; fetter moisture to flow to extracellular; reduce scattering and disappearing of moisture; also can improve the hydrophily of albuminate in addition, avoid albuminate to disturb metabolic activity.Along with the accumulation of proline in plant corpus, under adverse environmental factor, also there is regulating cell stable state, the electron stream of light stable reaction center, reduce the Xanthophyll cycle of Photosynthetic, reduce moisture loss.Abscisic acid under the impact of other composition can more effective promotion moisture absorption, reduce Water Transportation, and inducing specific albumen, form the Biological Free Radicals scavenger that more low-temperature adaptation albumen comprises SOD, cell is escaped injury, improve cold resistant property; Sow particularly crucially minimizing seed in advance and rest on the time in soil, avoid damage by disease and insect infecting seed in soil, PEG-4000 is conducive to the sprouting of seed under the impact of other composition, also helps the damage of repairing during seed storage, improves the germination rate of seed.In addition, the Ca of calcium chloride increase 2+ion is the required a kind of nutritive element of growth and development of plants, participates in the transmission of second messenger's signal of intracellular physiological biochemical reaction, improves stress resistance of plant, has important regulating and controlling effect to the growth of plant with growing; Boric acid can be used for insecticidal action, increases the raising of plant strain growth later stage quality and yield.In the present invention, the proportioning of proline, abscisic acid improves the tolerance to cold of seed, adds that the proportioning of PEG-4000 effectively improves seed germination speed.Can realize the early sowing of peanut seed, peanut seed is resisted cold, and surface temperature can also leave soil, fast germination after rising.The present invention can significantly improve peanut seed tolerance to cold, and meet cold current, chilling injury and the underproduction risk caused after resisting spring sowing, emergence rate significantly improves.
Peanut of the present invention resists cold seed treatment liquid field trial
1. not cold-resistant Screening of Germplasm
1.1 test material
Commercially available common peanut kind 50 parts.
1.2 test method
The distilled water of test proposes the previous day of precooling at 2 DEG C, gets 30 Kernel of Peanuts, is laid in and is added with on the culture dish of gauze; add rapidly the distilled water of precooling, process 96 hours at 2 DEG C, every part of seed repeats for 2 times; continue at then proceeding to 25 DEG C to cultivate, finally add up germination rate.
1.3 result of the test
Candidate go out germination rate lower than 2% not cold-resistant peanut kind.
2 field trials
2.1 test material
Through cold-resistant process germination rate lower than 2% peanut kind.
2.2 test method
Test employing three factor orthogonal regression experimental design method, design 23 groups of tests altogether, often organize 30 seeds, embodiment 1-6 treatment fluid and peanut ratio are that 1 seed adds treatment fluid 1-2ml, and peanut kind soaks 6-12h in treatment fluid.
2.3 field emergence investigation
Design 8 groups of tests, often organize 30 seeds, twice repetition, embodiment 1-6 and control group distilled water process process seed 6-12h, control group dry seeds is without any process.Liaoning Area monoseeding on April 20, in three days, (April 20, April 21, April 22) mean ground temperature is in 6 DEG C, with distilled water process and in contrast untreated.
Investigation on May 14 emergence rate, untreated dry seeds insemination and emergence rate is 35%, and insemination and emergence rate of only soaking seed is 53.3%, and embodiment 1-6 sows average emergence rate 82.5%.
Table 1 embodiment 1-6 and control group 1,2 laboratory and field statistics
As can be seen from Table 1, embodiment 1-6 entirety compared with control group 1,2 is better than control group.Because in embodiment 2, the proportioning of proline, abscisic acid improves the tolerance to cold of seed, add that the proportioning of PEG-4000 effectively improves seed germination speed.Can realize the early sowing of peanut seed, peanut seed is resisted cold, and surface temperature can also leave soil, fast germination after rising.Absolutely prove that the present invention can significantly improve peanut seed tolerance to cold, meet cold current, chilling injury and the underproduction risk caused after resisting spring sowing, emergence rate significantly improves.
Embodiment
In order to better the present invention is described, be further described below by embodiment, but special instruction is the scope that the application is not limited to following embodiment.
Embodiment 1
Take 1500mg proline, 0.15mg abscisic acid, 100000mg PEG-4000, calcium chloride 20mg, boric acid 5000mg, water 1000000mg in the present embodiment, stirred by above-mentioned raw materials, namely obtained peanut seed resists cold treatment fluid.
Embodiment 2
Take 1000mg proline, 0.1mg abscisic acid, 150000mg PEG-4000, calcium chloride 15mg, boric acid 2500mg, water 1000000mg in the present embodiment, stirred by above-mentioned raw materials, namely obtained peanut seed resists cold treatment fluid.
Embodiment 3
Take 500mg proline, 0.05mg abscisic acid, 200000mg PEG-4000, calcium chloride 10mg, boric acid 1000mg, water 1000000mg in the present embodiment, stirred by above-mentioned raw materials, namely obtained peanut seed resists cold treatment fluid.
Embodiment 4
Take the proline of 500mg, the abscisic acid of 0.15mg, the mg PEG-4000 of 200000, the water of 1000000mg in the present embodiment, stirred by above-mentioned raw materials, namely obtained peanut seed resists cold treatment fluid.
Embodiment 5
Take the proline of 1500mg, the abscisic acid of 0.05mg, the PEG-4000 of 100000mg, the water of 1000000mg in the present embodiment, stirred by above-mentioned raw materials, namely obtained peanut seed resists cold treatment fluid.
Embodiment 6
Take the proline of 1000mg, the abscisic acid of 0.10mg, the PEG-4000 of 150000mg, the water of 1000000mg in the present embodiment, stirred by above-mentioned raw materials, namely obtained peanut seed resists cold treatment fluid.

Claims (4)

1. peanut seed resists cold treatment fluid, it is characterized in that including following component:
The proline of 500-1500 weight portion, the abscisic acid of 0.05-0.15 weight portion, the weight portion PEG-4000 of 100000-200000, the water of 1000000 weight portions.
2. peanut seed according to claim 1 resists cold treatment fluid, it is characterized in that including following component:
The proline of 1000 weight portions, the abscisic acid of 0.10 weight portion, the PEG-4000 of 150000 weight portions, the water of 1000000 weight portions.
3. peanut seed according to claim 1 and 2 resists cold treatment fluid, characterized by further comprising the calcium chloride of 10-20 weight portion, the boric acid of 1000-5000 weight portion.
4. peanut seed according to claim 3 resists cold treatment fluid, characterized by further comprising the calcium chloride of 15 weight portions, the boric acid of 2500 weight portions.
CN201510366619.9A 2015-06-26 2015-06-26 Peanut seed resists cold treatment fluid Expired - Fee Related CN105028413B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106116829A (en) * 2016-06-22 2016-11-16 武汉农尚环境股份有限公司 A kind of freeze proof reagent of ornamental plant and compound method thereof
CN111359914A (en) * 2020-01-18 2020-07-03 山西大学 Device and method for screening crop seeds

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6150167A (en) * 1998-02-19 2000-11-21 Weyerhaeuser Company Method of determining conifer embryo maturity using sugar alcohol content
CN1358432A (en) * 2000-12-14 2002-07-17 中国科学院黑龙江农业现代化研究所 Cold-resistance agent for soybean
CN1973630A (en) * 2006-12-08 2007-06-06 浙江大学 Seed coating agent for raising cold resistance of super sweet corn seed
CN101642132A (en) * 2009-08-27 2010-02-10 华南农业大学 Cold-resistant seed soaking agent for direct sowing of rice as well as preparation method and applications thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6150167A (en) * 1998-02-19 2000-11-21 Weyerhaeuser Company Method of determining conifer embryo maturity using sugar alcohol content
CN1358432A (en) * 2000-12-14 2002-07-17 中国科学院黑龙江农业现代化研究所 Cold-resistance agent for soybean
CN1973630A (en) * 2006-12-08 2007-06-06 浙江大学 Seed coating agent for raising cold resistance of super sweet corn seed
CN101642132A (en) * 2009-08-27 2010-02-10 华南农业大学 Cold-resistant seed soaking agent for direct sowing of rice as well as preparation method and applications thereof

Non-Patent Citations (2)

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Title
FRANCOIS ROBERT LARHER ET AL: "Potentiation of praline accumulation in oilseed rape leaf discs exogenously supplied with combinations of PEG and cryoprotective agents is associated with overproduction of ABA", 《ENVIRONMENTAL AND EXPERIMENTAL BOTANY》 *
王宝增: "脯氨酸与植物的抗逆性", 《生物学教学》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106116829A (en) * 2016-06-22 2016-11-16 武汉农尚环境股份有限公司 A kind of freeze proof reagent of ornamental plant and compound method thereof
CN111359914A (en) * 2020-01-18 2020-07-03 山西大学 Device and method for screening crop seeds

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