CN105027984B - One kind cultivation high temperature resistant lung shape is picked up the ears method - Google Patents
One kind cultivation high temperature resistant lung shape is picked up the ears method Download PDFInfo
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- CN105027984B CN105027984B CN201510495956.8A CN201510495956A CN105027984B CN 105027984 B CN105027984 B CN 105027984B CN 201510495956 A CN201510495956 A CN 201510495956A CN 105027984 B CN105027984 B CN 105027984B
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- 210000004072 lung Anatomy 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 32
- 210000005069 ears Anatomy 0.000 title claims abstract description 31
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 28
- 239000001963 growth medium Substances 0.000 claims abstract description 18
- 241000894007 species Species 0.000 claims abstract description 17
- 239000000463 material Substances 0.000 claims abstract description 16
- 241000209140 Triticum Species 0.000 claims abstract description 15
- 235000021307 Triticum Nutrition 0.000 claims abstract description 15
- 239000010902 straw Substances 0.000 claims abstract description 15
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 14
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 10
- 238000003306 harvesting Methods 0.000 claims abstract description 10
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 7
- 240000001462 Pleurotus ostreatus Species 0.000 claims abstract description 5
- 230000035784 germination Effects 0.000 claims abstract description 5
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 2
- 230000004069 differentiation Effects 0.000 claims 1
- 230000000638 stimulation Effects 0.000 claims 1
- 241001138370 Pleurotus pulmonarius Species 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 238000002255 vaccination Methods 0.000 abstract description 2
- 230000003213 activating effect Effects 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000222485 Agaricales Species 0.000 description 1
- 241000222382 Agaricomycotina Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000004928 Paspalum scrobiculatum Species 0.000 description 1
- 235000003675 Paspalum scrobiculatum Nutrition 0.000 description 1
- 241001492261 Pleurotaceae Species 0.000 description 1
- 241000222350 Pleurotus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- FYHXNYLLNIKZMR-UHFFFAOYSA-N calcium;carbonic acid Chemical compound [Ca].OC(O)=O FYHXNYLLNIKZMR-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
One kind cultivation high temperature resistant lung shape is picked up the ears (Pleurotus pulmonarius (Fr.) Qu é l) method, and this method includes original seed and parent species making, cultigen making, bacterium germination, management of producing mushroom step.The high warm lung shape oyster mushroom strain CCMSSC00499 for taking national Culture Collection is material, use PDA culture medium activate after for parent species, original seed is made with wheat culture medium, finally using 80 90% straw, 9 18% wheat brans and calcium carbonate 1 2% make cultivating bag；After traditional vaccination, cultivated in 20 28 DEG C 25 35 days and all cover with cultivating bag to mycelia, finally in 28 32 DEG C of temperature, relative air humidity is cultivates 7 10 days to harvesting under conditions of 80% 95%.The invention provides the cultural method that a kind of high warm lung shape is picked up the ears, it is therefore intended that provides a new culture under high temperature bacterial strain for mushroom industry, enriches germ plasm resource.
Description
Technical field
The invention belongs to biological technical field, and in particular to the cultural method that a kind of edible mushroom high temperature resistant lung shape is picked up the ears, bag
Compost is included with when management of producing mushroom step etc..
Background technology
Lung shape picks up the ears (Pleurotus pulmonarius (Fr.) Qu é l), trade name phoenix-tail mushroom, belongs on taxology
Basidiomycotina, Hymenomycetes, Agaricales, Pleurotaceae, Pleurotus, it is one of most important traditional cultivation kind in China, main point
Cloth is in Henan, Guangxi, Guangdong, Shaanxi, Tibet.Lung shape picks up the ears not only to have that growth is fast, yield is high, delicious flavour and nutritious
The features such as, also containing several amino acids and vitamin, and its physiological activator has raising immune function of human body, anti-cancer, resists
The effect of cancer.Current high warm lung shape is picked up the ears, and planting technology difficulty is big, and success rate is very low.Want to make high warm lung shape to pick up the ears high yield, steady
Production, grasps the correct manufacture craft of strain, ensures that the good quality of strain is critically important.It was verified that breeding increases production than inferior strain
More than 30%, and fruiting is strong, and pest and disease damage seldom occurs.At present, the patent application picked up the ears on lung shape, which is detected in utilize, to divide
Son mark distinguishes the method (application number 200810072137.2) of phoenix-tail mushroom and oyster cap fungus, high temperature pick up the ears cultivation it is existing largely into
Work(reports, such as all guests propose high temperature and picked up the ears the formula (application number of parent species formula, original seed formula and planting material
200910182143.8；Application number 200910182145.7；Application number 200910182144.2).But use cultivation using staw high temperature
Lung shape is picked up the ears there is not yet relevant report.
The content of the invention
It is an object of the invention to provide the cultural method that a kind of high temperature resistant lung shape is picked up the ears.Using this method cultivation lung shape side
Ear, the Utilizing question of rice straw is not only solved, be also greatly reduced the cost of raw material, improve the economic effect of the producer
Benefit.
The above-mentioned purpose of the present invention is realized by following technical schemes：
A kind of method cultivated high temperature resistant lung shape and picked up the ears, this method includes original seed and parent species make, cultigen makes, hair
Bacterium, management of producing mushroom step, it is parent species to take the lung shape that culture presevation numbering is CCMSSC00499 to pick up the ears in activation in PDA culture medium,
Cultigen is made using wheat culture medium again, finally cultigen is inoculated into the cultivating bag equipped with aseptic culture material, bacterium germination, gone out
Mushroom is managed to harvesting.
Picked up the ears cultural method according to described high temperature resistant lung shape, wherein the formula of the aseptic culture material is：80-90% rice
Grass, 9-18% wheat brans and calcium carbonate 1-2%, water content 60-65%.
Picked up the ears cultural method according to described high temperature resistant lung shape, wherein the bacterium germination and fruiting step are cultigen in 20-
After 28 DEG C of cultures all cover with cultivating bag in 25-35 days to mycelia, in 28-32 DEG C of temperature, relative air humidity is 80%-95%'s
Under the conditions of cultivate 7-10 days harvest.
Picked up the ears cultural method according to described high temperature resistant lung shape, wherein the wheat culture medium is 98% wheat, 2% carbonic acid
Calcium.
Picked up the ears cultural method according to described high temperature resistant lung shape, wherein methods described is with high warm lung shape oyster mushroom strain
CCMSSC00499 is material, use PDA culture medium activate after for parent species, original seed is made with wheat culture medium, finally using 80-
90% straw, 9-18% wheat brans and calcium carbonate 1-2% make cultigen, load cultivating bag；After traditional vaccination, in 20-28 DEG C of training
Support 25-35 days and all cover with cultivating bag to mycelia, finally in 28-32 DEG C of temperature, relative air humidity is 80%-95% condition
It is lower to cultivate 7-10 days to harvesting.
The present invention is picked up the ears the isolated high warm lung shape of fructification by the high temperature resistant lung shape selected from fruiting testing sieve
Novel bacterial bacterial strain CCMSSC00499-001 pick up the ears as cultivation object, there is provided a kind of cost is cheap, process is simple, repeatability is high, week
Phase is shorter, is easily managed the cultural method that the high warm lung shape of maintenance is picked up the ears, and the cultural method can be utilized in arviculture often
The waste material seen, and can improve agricultural production efficiency.
Embodiment
The present invention is further described below in conjunction with specific embodiment, but embodiment is merely to illustrate, and can not be limited
The scope of the invention processed.
Embodiment 1：
The high temperature resistant lung shape cultural method of picking up the ears of the present invention generally includes：With high warm lung shape oyster mushroom strain CCMSSC00499
For material, use PDA culture medium activate after for parent species, be made original seed with wheat culture medium, wheat culture medium be 98% wheat,
2% calcium carbonate.Cultigen is finally made using 80-90% straw, 9-18% wheat brans and calcium carbonate 1-2%, loads cultivating bag；Often
After rule inoculation, cultivating bag is all covered with to mycelia within 25-35 days in 20-28 DEG C of culture, finally in 28-32 DEG C of temperature, air is relative
Humidity cultivates 7-10 days to harvesting under conditions of being 80%-95%.
This method concretely comprises the following steps：
Original seed makes：The lung shape that culture presevation numbering is CCMSSC00499 is taken to pick up the ears (Pleurotus pulmonarius
(Fr.) Qu é l) in activating 7 days in common PDA culture medium it is parent species.Parent species are inoculated into wheat 98%, on calcium carbonate 2% again,
Lucifuge culture 28 days, are made cultigen at 25 DEG C.
Planting material makes：Whole straw is cut into 3cm or so straw section, by straw 80%, wheat bran 18%, calcium carbonate 2%
Ratio is mixed thoroughly, water content 65%, autoclave sterilization 2 hours, is fitted into stand-by in cultivating bag.
Implantation methods：Above-mentioned cultigen is cultivated into the cultivating bag equipped with planting material, cultivated using stack, 3 layers,
25 DEG C, lucifuge culture 26 days.
Manage and protect method：30 DEG C, illumination 200lux of temperature, relative air humidity cultivate 7 days to harvesting under conditions of being 90%.
Embodiment 2：
Operated with reference to the overall plan of embodiment 1.
Original seed makes：The lung shape that culture presevation numbering is CCMSSC00499 is taken to pick up the ears (Pleurotus pulmonarius
(Fr.) Qu é l) in activating 7 days in common PDA culture medium it is parent species.Parent species are inoculated into wheat 98%, on calcium carbonate 2% again,
Lucifuge culture 25 days, are made cultigen at 25 DEG C, are fitted into stand-by in cultivating bag.
Planting material makes：Whole straw is cut into 3cm or so straw section, compared by straw 90%, wheat bran 8%, calcium carbonate 2%
Example is mixed thoroughly, water content 65%, autoclave sterilization 2 hours.
Implantation methods：Above-mentioned cultigen is cultivated into the cultivating bag equipped with planting material, cultivated using stack, typically
4 layers, 25 DEG C, lucifuge culture 30 days.
Manage and protect method：32 DEG C, illumination 250lux of temperature, relative air humidity cultivate 8 days to harvesting under conditions of being 90%.
Embodiment 3：
Operated with reference to the overall plan of embodiment 1.
Original seed makes：The lung shape that culture presevation numbering is CCMSSC00499 is taken to pick up the ears (Pleurotus pulmonarius
(Fr.) Qu é l) in activating 7 days in common PDA culture medium it is parent species.Parent species are inoculated into wheat 98%, on calcium carbonate 2% again,
Lucifuge culture 28 days, are made cultigen at 25 DEG C, are fitted into stand-by in cultivating bag.
Planting material makes：Whole straw is cut into 3cm or so straw section, by straw 85%, wheat bran 14%, calcium carbonate 1%
Ratio is mixed thoroughly, water content 65%, autoclave sterilization 2 hours.
Implantation methods：Above-mentioned cultigen is cultivated into the cultivating bag equipped with planting material, cultivated using stack, typically
5 layers, 28 DEG C, lucifuge culture 28 days.
Manage and protect method：35 DEG C, illumination 150lux of temperature, relative air humidity cultivate 8 days to harvesting under conditions of being 85%.
Claims (3)
- A kind of 1. method cultivated high temperature resistant lung shape and picked up the ears, it is characterised in that this method includes parent species making, cultigen makes, hair Bacterium, management of producing mushroom step, the high temperature resistant lung shape that culture presevation numbering is CCMSSC00499-001 is taken to pick up the ears in PDA culture medium Activate as parent species, then cultigen is made using wheat culture medium, cultigen is finally inoculated into the cultivation equipped with aseptic culture material In bag, bacterium germination, management of producing mushroom to harvesting, the formula of the aseptic culture material are：85% straw, 14% wheat bran and calcium carbonate 1%, Water content 65%, the bacterium germination and fruiting step are that cultigen is cultivated 25-35 days at 20-28 DEG C and all covers with cultivating bag to mycelia Afterwards, in 28-32 DEG C of temperature, relative air humidity is cultivated 7-10 days under conditions of being 80%-95% and harvested, the wheat culture medium For 98% wheat and 2% calcium carbonate.
- The cultural method 2. high temperature resistant lung shape according to claim 1 is picked up the ears, it is characterised in that the cultigen mycelium training It is 26 DEG C to support temperature, and fruiting body differentiation temperature is 30 DEG C, and fruiting stage air relative humidity is 90%.
- The cultural method 3. high temperature resistant lung shape according to claim 1 is picked up the ears, it is characterised in that methods described is with high temperature resistant Lung shape oyster mushroom strain CCMSSC00499-001 is material, is activated 7 days using PDA culture medium lucifuge and obtains parent species, parent species are inoculated with Cultigen is made to wheat culture medium, is planting material with 85% straw, 14% wheat bran and calcium carbonate 1%, loads cultivating bag；It is conventional After inoculation, cultivated in 26 DEG C 25-35 days and all cover with cultivating bag to mycelia, opening sack after mycelium stimulation is placed in 30 DEG C of temperature, air Relative humidity cultivates 7-10 days to harvesting under conditions of being 90%.
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| CN107736190A (en) * | 2017-11-13 | 2018-02-27 | 云南菌视界生物科技有限公司 | A kind of waste material after fruiting using schizophyllum commune cultivates the method that high warm lung shape is picked up the ears |
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| CN1065176A (en) * | 1991-12-03 | 1992-10-14 | 刘德礼 | A kind of method of making flat mushroom, phoenix-tail mushroom bacterial classification |
| CN101759457A (en) * | 2008-11-19 | 2010-06-30 | 赵春 | High yield method utilizing straw mixture to cultivate phoenix-tail mushroom |
| CN103125261A (en) * | 2009-06-12 | 2013-06-05 | 镇江市食用菌研究所 | Method to interplant phoenix mushrooms in biological sunshelter in summer |
| CN103319234A (en) * | 2013-06-03 | 2013-09-25 | 长沙湘新农业科技有限公司 | Pleurotus geesteranus medium and its cultivation method |
| CN103918475B (en) * | 2014-03-26 | 2016-04-27 | 上海惠芬果蔬专业合作社 | The elegant precious method of mushroom bonsai type cultivation and the medium for cultivating elegant precious mushroom |
| CN104663250A (en) * | 2015-03-12 | 2015-06-03 | 象州县科学技术局 | Method for cultivating phoenix mushroom with mulberry twigs |
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