CN105018378A - Complex microbial inoculant for purifying anaerobic fermentation waste gas containing benzene series and preparation method and purpose of complex microbial inoculant - Google Patents
Complex microbial inoculant for purifying anaerobic fermentation waste gas containing benzene series and preparation method and purpose of complex microbial inoculant Download PDFInfo
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- CN105018378A CN105018378A CN201510416898.5A CN201510416898A CN105018378A CN 105018378 A CN105018378 A CN 105018378A CN 201510416898 A CN201510416898 A CN 201510416898A CN 105018378 A CN105018378 A CN 105018378A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
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Abstract
The invention discloses a complex microbial inoculant for purifying anaerobic fermentation waste gas containing benzene series and a preparation method and purpose of the complex microbial inoculant. The complex microbial inoculant comprises methane-oxidizing bacteria such as Methylosinus trichosporium, Methylosinus sporium, Methylocystis sp. and Methylosinus parvus and benzene series degrading bacteria such as Azoarcus indigens, Terrimonas sp., Pseudomonas aeruginosa, Bacillus sp., Ralstonia sp. And Runella sp.. The complex microbial inoculant has the advantages that the complex microbial inoculant can purify the anaerobic fermentation waste gas containing methane and benzene series, is applicable to repairing of sites polluted by the benzene series and purifying of landfill gas of refuse landfills, has certain economic efficiency and environmental protection significance, and is low in production cost, convenient to use and free of secondary pollution to environments.
Description
Technical field
The invention belongs to microbiobacterial agent preparation field, relate to a kind of purification and contain composite fungus agent of benzene homologues anaerobically fermenting waste gas and its production and use.
Technical background
Domestic refuse, waste residue and waste liquid also can produce a large amount of fermentation waste gas in anaerobically fermenting treating processes, and its component comprises methane, carbonic acid gas and multiple volatile organic contaminant (VOCs).Benzene homologues (BTEX comprises benzene, toluene, ethylbenzene, dimethylbenzene) is wherein comparatively common and main VOCs.In the soil that some are petroleum-polluted, petroleum pollution common contaminant BTEX and the methane produced by deep subsoil microbiological anaerobic degradation of organic substances are present in soil gas simultaneously, enter air by the Transport And Transformation in soil.Wherein, methane has very strong Greenhouse effect; And BTEX has stronger bio-toxicity and lower smelly threshold value, be defined as strong carcinogenic substance by the World Health Organization, be positioned on pollutent priority acccess control list.Larger risk is all there is in the release of this kind of waste gas to surrounding enviroment and HUMAN HEALTH.Therefore, research and development can have great importance to the environment protection of these particular places and HUMAN HEALTH containing the technology of BTEX anaerobically fermenting waste gas by high-efficient purification.
At present, purification conventional both at home and abroad comprises condensation method, catalytic burning method, absorption method, solution absorption method and biological degradation method etc. containing the method for BTEX waste gas, in addition, some emerging technology, as photocatalytic conversion method, plasma method, pulse ionization technique etc. are still in development.Traditional materializing strategy ubiquity working cost is high, the problem that secondary pollution is serious.In the organic exhaust gas that biological process is large-minded in process, concentration is low, biological degradability is good, there is certain advantage.
Purification biotechnology, can be divided into by the difference of the addition manner of the existing way of microorganism and moisture, nutrition: biofilter, bio-trickling filter and biological scrubber three class.Wherein, biological scrubber is made up of gas wash tower and active sludge regenerated reactor usually, is applicable to that load is high, the water-soluble larger situation of pollutent.Biofilter is a kind of cleaning equipment for waste organic gas of filling the pond shape structure with the moist filtrate of adsorptivity wherein.Traditional biological filter method also exists that reaction conditions is as wayward in pH, nutrition etc. in actual applications, filler degraded needs renewal, the too high easy blocking of load, filler mummification, poorly water-soluble the problems such as compound treatment efficiency is low.Bio-trickling filter, on the basis of biofiltration, arranges circulating sprayer at top, can be the nutritive element such as nitrogen, phosphorus that microbiological deterioration organism provides required.For obtaining efficient BTEX decontamination effect improving, in biological filter, inoculation has the microbial preparation of benzene homologues degradation function or spray containing the nutrient solution of microbial preparation of benzene homologues degradation function is method comparatively conventional at present.But system extended with working time, there will be the phenomenon of the clearance decline caused because system carbon source is not enough.Although there is abundant carbon source in fermentation waste gas, benzene homologues degradation bacteria can not directly utilize methane as its carbon source.Therefore, these current microbial preparations all need additionally to add carbon source and it could be kept the removal effect of BTEX, and can not realize the effective removal to wherein methane.The intermediate product of methane-oxidizing bacteria metabolize methane, as methyl alcohol, is that a class is more easily by carbon source material that non-methane oxidation bacteria utilizes.Therefore, improve the discharge that system methane oxidation ability not only can reduce greenhouse gas methane, also can strengthen the removal effect of system to BTEX.But, do not have methane oxidation ability for the microbial preparation containing BTEX waste gas at present, thus effectively can not reduce the Greenhouse effect that exhaust gas emission brings, also can not keep purifying the economical and efficient of BTEX in anaerobically fermenting waste gas.
For the problems referred to above, develop a kind of can simultaneously in efficient degradation anaerobism waste gas the composite fungus agent of methane and BTEX seem particularly important.
Summary of the invention
The technical problem to be solved in the present invention is exactly, the problem coexisted containing multiple gases in the purification of BTEX anaerobically fermenting waste gas and BTEX contaminated site can not be solved for current existing removal containing the biotechnological formulation of BTEX waste gas comprehensively, provide a kind of efficient purification containing the composite fungus agent of the anaerobically fermenting waste gas of BTEX.Another object of the present invention is to provide the concrete preparation method and its usage of this complex micro organism fungicide.
To achieve these goals, concrete scheme of the present invention is:
Purification is containing a composite fungus agent for benzene homologues anaerobically fermenting waste gas, and this composite fungus agent contains methane-oxidizing bacteria and benzene homologues degradation bacteria, and the number ratio of methane-oxidizing bacteria and benzene homologues degradation bacteria is (2 ~ 5): 1;
Wherein, described methane-oxidizing bacteria comprises
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvus, its number ratio is (35 ~ 45): (3 ~ 10): (2 ~ 8): 1;
Described benzene homologues degradation bacteria comprises
azoarcus indigens,
terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp., its number ratio is 1:(4 ~ 8): (4 ~ 8): (2 ~ 6): (2 ~ 6): (2 ~ 6).
Further, in described composite fungus agent, total cell concn of methane-oxidizing bacteria and benzene homologues degradation bacteria is at least 0.3 mg
dry weight/ mL.
A kind of preparation method for purifying the composite fungus agent containing benzene homologues anaerobically fermenting waste gas, be specially by methane-oxidizing bacteria mixed bacteria liquid and benzene homologues degradation bacteria mixed bacteria liquid under aerobic condition by number ratio (2 ~ 5): encapsulation after 1 mixing, and under 2-8 ° of C freezen protective, obtain described for purifying the composite fungus agent containing benzene homologues anaerobically fermenting waste gas;
Wherein, described methane-oxidizing bacteria mixed bacteria liquid is for containing
methylosinus trichosporium,
methylosinus sporium,
methylocystissp. and
methylocystis parvusthe mixed bacteria liquid of four kinds of methane-oxidizing bacterias;
Described benzene homologues degradation bacteria mixed bacteria liquid is for containing
azoarcus indigens,
terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. the mixed bacteria liquid of six kinds of benzene homologues degradation bacteria.
Further, described methane-oxidizing bacteria mixed bacteria liquid preparation method is: by described
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvusfour kinds of methane-oxidizing bacterias are individually inoculated in the culture tank containing the nitrate inorganic medium of sterilizing, inject high-purity methane and oxygen, make methane and the concentration of oxygen in culture tank head space be respectively 10% ~ 20%(v/v), 18% ~ 21% (v/v), individually carry out enlarged culturing step by step, obtain corresponding single bacterium liquid, namely respective methane-oxidizing bacteria seed liquor, will
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvusfour kinds of methane-oxidizing bacteria seed liquor are by bacterium number ratio (35 ~ 45): (3 ~ 10): (2 ~ 8): 1 mixing, obtains methane-oxidizing bacteria mixed bacteria liquid.
Further, described benzene homologues degradation bacteria mixed bacteria liquid preparation method is:
Aseptically, will to have activated
azoarcus indigens, Terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. bacterium liquid is individually inoculated in the culture tank containing sterilized nitrate inorganic medium, after culture tank sealing, inject benzene homologues, make Benzene series Concentrations in headspace gas be 0.6-1.0%(v/v), in 30 ~ 35 ° of C, carry out enlarged culturing step by step under 100 ~ 150 rpm and obtain
azoarcus indigens, Terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. respective seed liquor;
Will
azoarcus indigens,
terrimonassp.
, Pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. six kinds of seed liquor are by bacterium number ratio 1:(4 ~ 8): (4 ~ 8): (2 ~ 6): (2 ~ 6): (2 ~ 6) are mixed to get benzene homologues degradation germ liquid.
Further, the formula of described nitrate inorganic medium is: NaNO
385 mg/L, Na
2sO
4142 mg/L, K
2hPO
43H
2o 1368 mg/L, KH
2pO
4h
2o 600.6 mg/L, CaCl
25.3 mg/L, FeSO
45H
2o 9.7 mg/L, ZnSO
47H
2o 0.6 mg/L, MgSO
47H
2o 36.9 mg/L, MnSO
4h
2o 0.44 mg/L, CuSO
46H
2o 0.27 mg/L, KI 0.17 mg/L, H
3bO
30.37 mg/L, CoCl
26H
2o 0.09 mg/L, NaMoO
42H
2o 0.09 mg/L, NaFeEDTA salt 3.8 mg/L, pH are 6 ~ 8; The incubation time of every one-level of enlarged culturing is 4 ~ 8 days step by step.
Use a purposes for described composite fungus agent, for purifying methane in anaerobically fermenting waste gas and benzene homologues simultaneously, wherein benzene homologues is one or more in benzene, toluene, ethylbenzene and dimethylbenzene.
Further, the concrete grammar that microbial inoculum is used for purifying methane in anaerobically fermenting waste gas and benzene homologues is simultaneously: pass in biological filter by the anaerobically fermenting waste gas containing methane and benzene homologues, and described composite fungus agent and nitrate inorganic medium are pressed the dilution proportion of 1:10 ~ 1:15, be sprayed at the surface of biological filter, methane and benzene homologues are degraded in biological filter.
beneficial effect:
Compared to prior art, the invention provides a kind of composite fungus agent that simultaneously can purify greenhouse gas methane in anaerobically fermenting and BTEX.In this microbial inoculum, methane-oxidizing bacteria can not only oxidizing of methylene, and can produce methane carbon, the growth of non-methane oxidation bacteria in Promotion system, improves the degraded of BTEX.In addition, this microbial inoculum can make up existing purification BTEX waste gas biotechnological formulation and only remove BTEX, and can not remove the defect of greenhouse gas methane simultaneously.This products production cost is low, easy to use, to environment non-secondary pollution, can be used for the purification etc. of landfill gas in the reparation of BETX contaminated site and refuse landfill, has certain economy and environment protection significance.
Accompanying drawing explanation
Fig. 1 is the treatment effect figure of embodiments of the invention 1;
Fig. 2 is the treatment effect figure of embodiments of the invention 2.
Embodiment
Below in conjunction with embodiment, the invention will be further described.Methane-oxidizing bacteria of the present invention (
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvus) and BTEX degradation bacteria (
azoarcusindigens,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp.
, Terrimonassp. and
runellasp.) be existing bacterial classification, can buy from American Type Culture collection warehousing (American type culture collection, ATCC).
Purification is containing a composite fungus agent for benzene homologues anaerobically fermenting waste gas, and this composite fungus agent contains methane-oxidizing bacteria and benzene homologues degradation bacteria, and the number ratio of methane-oxidizing bacteria and benzene homologues degradation bacteria is (2 ~ 5): 1;
Wherein, described methane-oxidizing bacteria comprises
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvus, its number ratio is (35 ~ 45): (3 ~ 10): (2 ~ 8): 1;
Described benzene homologues degradation bacteria comprises
azoarcus indigens,
terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp., its number ratio is 1:(4 ~ 8): (4 ~ 8): (2 ~ 6): (2 ~ 6): (2 ~ 6).
Further, in described composite fungus agent, total cell concn of methane-oxidizing bacteria and benzene homologues degradation bacteria is at least 0.3 mg
dry weight/ mL.
A kind of preparation method for purifying the composite fungus agent containing benzene homologues anaerobically fermenting waste gas, be specially by methane-oxidizing bacteria mixed bacteria liquid and benzene homologues degradation bacteria mixed bacteria liquid under aerobic condition by number ratio (2 ~ 5): encapsulation after 1 mixing, and under 2-8 ° of C freezen protective, obtain described for purifying the composite fungus agent containing benzene homologues anaerobically fermenting waste gas;
Wherein, described methane-oxidizing bacteria mixed bacteria liquid is for containing
methylosinus trichosporium,
methylosinus sporium,
methylocystissp. and
methylocystis parvusthe mixed bacteria liquid of four kinds of methane-oxidizing bacterias;
Described benzene homologues degradation bacteria mixed bacteria liquid is for containing
azoarcus indigens,
terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. the mixed bacteria liquid of six kinds of benzene homologues degradation bacteria.
Further, described methane-oxidizing bacteria mixed bacteria liquid preparation method is: by described
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvusfour kinds of methane-oxidizing bacterias are individually inoculated in the culture tank containing the nitrate inorganic medium of sterilizing, inject high-purity methane and oxygen, make methane and the concentration of oxygen in culture tank head space be respectively 10% ~ 20%(v/v), 18% ~ 21% (v/v), individually carry out enlarged culturing step by step, obtain corresponding single bacterium liquid, namely respective methane-oxidizing bacteria seed liquor, will
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvusfour kinds of methane-oxidizing bacteria seed liquor are by bacterium number ratio (35 ~ 45): (3 ~ 10): (2 ~ 8): 1 mixing, obtains methane-oxidizing bacteria mixed bacteria liquid.
Further, described benzene homologues degradation bacteria mixed bacteria liquid preparation method is:
Aseptically, will to have activated
azoarcus indigens, Terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. bacterium liquid is individually inoculated in the culture tank containing sterilized nitrate inorganic medium, after culture tank sealing, inject benzene homologues, make Benzene series Concentrations in headspace gas be 0.6-1.0%(v/v), in 30 ~ 35 ° of C, carry out enlarged culturing step by step under 100 ~ 150 rpm and obtain
azoarcus indigens, Terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. respective seed liquor;
Will
azoarcus indigens,
terrimonassp.
, Pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. six kinds of seed liquor are by bacterium number ratio 1:(4 ~ 8): (4 ~ 8): (2 ~ 6): (2 ~ 6): (2 ~ 6) are mixed to get benzene homologues degradation germ liquid.
Further, the formula of described nitrate inorganic medium is: NaNO
385 mg/L, Na
2sO
4142 mg/L, K
2hPO
43H
2o 1368 mg/L, KH
2pO
4h
2o 600.6 mg/L, CaCl
25.3 mg/L, FeSO
45H
2o 9.7 mg/L, ZnSO
47H
2o 0.6 mg/L, MgSO
47H
2o 36.9 mg/L, MnSO
4h
2o 0.44 mg/L, CuSO
46H
2o 0.27 mg/L, KI 0.17 mg/L, H
3bO
30.37 mg/L, CoCl
26H
2o 0.09 mg/L, NaMoO
42H
2o 0.09 mg/L, NaFeEDTA salt 3.8 mg/L, pH are 6 ~ 8; The incubation time of every one-level of enlarged culturing is 4 ~ 8 days step by step.
Use a purposes for described composite fungus agent, for purifying methane in anaerobically fermenting waste gas and benzene homologues simultaneously, wherein benzene homologues is one or more in benzene, toluene, ethylbenzene and dimethylbenzene.
Further, microbial inoculum is used for purifying the concrete grammar of methane in anaerobically fermenting waste gas and benzene homologues simultaneously, namely the using method of microbial inoculum is: pass in biological filter by the anaerobically fermenting waste gas containing methane and benzene homologues, and described composite fungus agent and nitrate inorganic medium are pressed the dilution proportion of 1:10 ~ 1:15, be sprayed at the surface of biological filter, once a day, 3 ~ 5 days are sprayed continuously.Waste gas, by the solution absorbs containing microbial inoculum of the filter material surface in biological filter, makes methane and benzene homologues be degraded by microorganisms in biological filter, reaches waste-gas cleaning object.
embodiment 1
Step 1: obtained methane-oxidizing bacteria mixed bacteria liquid.
Respectively by activated methane-oxidizing bacteria (
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvusdeng) be inoculated into and be equipped with in the culture tank of the nitrate inorganic medium of 120 ° of C high-temperature sterilizations, inject methane and oxygen simultaneously, its content is made to be respectively 10%(v/v), 21% (v/v), at 30 ° of C, cultivate under rotating speed 100 rpm, cultivate until bacterial concentration reaches 0.4 mg dry weight/mL,, the obtained methane-oxidizing bacteria seed liquor with high-efficiency methane oxidation capacity.By four kinds of methane-oxidizing bacterias (
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvus) seed liquor by number ratio 45:3:2:1 mixing, obtained methane-oxidizing bacteria mixed solution.
Step 2: obtained BTEX degradation bacteria mixed bacteria liquid.
Aseptically, will to have activated respectively
azoarcusindigens,
terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. waiting bacterium liquid to be inoculated in is equipped with in the culture tank of the nitrate inorganic medium of 120 ° of C high-temperature sterilizations, after culture tank sealing, add a certain amount of BTEX wherein, BTEX concentration in headspace gas is made to be 0.6%(v/v), in 30 ° of C, cultivate under rotating speed 100 rpm, cultivate until bacterial concentration reaches 0.4 mg dry weight/mL, obtained can efficient degradation BTEX
azoarcusindigens, Terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. six kinds of degradation of toluene bacterium seed liquor.
Will
azoarcus indigens,
terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. six kinds of seed liquor are by number ratio 1:8:8:6:6:6 mixing, obtained BTEX degradation bacteria mixed-culture medium.
Step 3: aseptically, the ratio of group mixed bacteria liquid of 2 described in step 1 and step 2 according to number ratio 5:1 mixed, bacterial concentration reaches 0.4 mg
dry weight/ mL, encapsulation, obtained purification is containing BTEX anaerobically fermenting waste gas composite fungus agent.
Composite fungus agent effect test:
The decontamination effect improving of biotechnological formulation to the anaerobically fermenting waste gas containing BTEX of the present invention and commercially available conventional removal BTEX waste gas contrasts.The biotechnological formulation called after T of the removal BTEX waste gas selected, main component is
burkholderia,
flavobacteria,
azoarcuswith
microbacterium.Activated in nitrate inorganic medium by reference substance, adding BTEX to concentration is 0.6%(v/v), be cultured to bacterial concentration and reach 0.4 mg
dry weight/ mL, this product (called after MT) and reference substance are inoculated in identical amount in the culture tank that sterilized substratum is housed, after sealing, inject the anaerobically fermenting waste gas containing BTEX of identical amount wherein, wherein methane content 50%(v/v), BTEX content 1%(v/v), culture tank is positioned over 30 ° of C, vibrate under rotating speed 100 rpm, every 12 h measure the content of culture tank head space methane and BTEX, ventilation 1 h is carried out after mensuration, then refill containing BTEX waste gas, wherein methane content 50%(v/v), BTEX content 1%(v/v), culture tank is positioned over 30 ° of C, vibrate under rotating speed 100 rpm, every 12 h measure the content of culture tank head space methane and BTEX, so repeat cultivation 10 days, relatively in incubation period this product and reference substance to the clearance of methane in waste gas and BTEX and degradation rate, test-results as shown in Figure 1.Result shows, biological reagent T only possesses BTEX degradation capability and do not possess methane oxidation ability, and its BTEX average removal rate is 85%, and the highest degradation rate is 202 μm of ol/Lh; This product then possesses the ability removing methane and BTEX simultaneously, and its methane and BTEX average removal rate can reach 90% and 95% respectively, and the highest degradation rate can reach 180 and 285 μm of ol/Lh respectively.。
embodiment 2
Four kinds of methane-oxidizing bacterias are individually at methane content 20%(v/v), oxygen content 18%(v/v), 35 ° of C, carry out enlarged culturing step by step under rotating speed 150 rpm, are 0.4 mg to often kind of methane-oxidizing bacteria bacterial concentration
dry weight/ mL, four kinds of methane-oxidizing bacterias are 35:10:8:1 mixing by number ratio; BTEX degradation bacteria
azoarcus indigens1%(v/v is respectively at BTEX and oxygen content) and 5%(v/v), 35 ° of C, carry out enlarged culturing step by step under rotating speed 150 rpm, are 0.4 mg to bacterial concentration
dry weight/ mL;
terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. individually 1%(v/v is respectively at BTEX and oxygen content) and 21%(v/v), 35 ° of C, carry out enlarged culturing step by step under rotating speed 150 rpm, are 0.4 mg to often kind of BTEX degradation germ liquid concentration
dry weight/ mL, six kinds of BTEX degradation bacteria are by number ratio 1:4:4:2:2:2 mixing; Methane-oxidizing bacteria mixed bacteria liquid mixes by number ratio 2:1 with BTEX degradation bacteria mixed bacteria liquid, and other culturing steps as described in example 1 above.
Composite fungus agent effect test:
The decontamination effect improving of biotechnological formulation T to the anaerobically fermenting waste gas containing different concns BTEX and methane that the present invention is identical with described in embodiment 1 contrasts.Wherein, in gas phase, BTEX concentration is set as 0.2%, 0.6%, 0.8% respectively, and methane concentration is 50%, and test-results as shown in Figure 2.Result shows, and biotechnological formulation T only possesses BTEX removal ability, and it is respectively 82%, 86%, 85% to BTEX average removal rate, and the highest degradation rate is respectively 38,109,158 μm of ol/Lh.The present invention can remove methane and BTEX simultaneously.Wherein methane clearance is respectively 98%, 93% and 90%, and the highest methane oxidation speed is respectively 212,200 and 193 μm of ol/Lh.The present invention, under different B TEX concentration, is respectively the highest degradation rate of 94%, 93% and 95%, BTEX to BTEX clearance and is respectively 59,180 and 230 μm of ol/Lh.
Below by reference to the accompanying drawings the specific embodiment of the present invention is described; but these explanations can not be understood to limit scope of the present invention; protection scope of the present invention is limited by the claims of enclosing, and any change on the claims in the present invention basis is all protection scope of the present invention.
Claims (8)
1. purification is containing a composite fungus agent for benzene homologues anaerobically fermenting waste gas, and it is characterized in that, this composite fungus agent contains methane-oxidizing bacteria and benzene homologues degradation bacteria, and the number ratio of methane-oxidizing bacteria and benzene homologues degradation bacteria is (2 ~ 5): 1;
Wherein, described methane-oxidizing bacteria comprises
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvus, its number ratio is (35 ~ 45): (3 ~ 10): (2 ~ 8): 1;
Described benzene homologues degradation bacteria comprises
azoarcus indigens,
terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp., its number ratio is 1:(4 ~ 8): (4 ~ 8): (2 ~ 6): (2 ~ 6): (2 ~ 6).
2. a kind of composite fungus agent for purifying containing benzene homologues anaerobically fermenting waste gas according to claim 1, is characterized in that, in described composite fungus agent, total cell concn of methane-oxidizing bacteria and benzene homologues degradation bacteria is at least 0.3 mg
dry weight/ mL.
3. one kind for purifying the preparation method of the composite fungus agent containing benzene homologues anaerobically fermenting waste gas, it is characterized in that, methane-oxidizing bacteria mixed bacteria liquid and benzene homologues degradation bacteria mixed bacteria liquid are pressed number ratio (2 ~ 5) under aerobic condition: encapsulation after 1 mixing, and under 2-8 ° of C freezen protective, obtain described for purifying the composite fungus agent containing benzene homologues anaerobically fermenting waste gas;
Wherein, described methane-oxidizing bacteria mixed bacteria liquid is for containing
methylosinus trichosporium,
methylosinus sporium,
methylocystissp. and
methylocystis parvusthe mixed bacteria liquid of four kinds of methane-oxidizing bacterias;
Described benzene homologues degradation bacteria mixed bacteria liquid is for containing
azoarcus indigens,
terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. the mixed bacteria liquid of six kinds of benzene homologues degradation bacteria.
4. as claimed in claim 3 for purifying the preparation method of the composite fungus agent containing benzene homologues anaerobically fermenting waste gas, it is characterized in that, described methane-oxidizing bacteria mixed bacteria liquid preparation method is: by described
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvusfour kinds of methane-oxidizing bacterias are individually inoculated in the culture tank containing the nitrate inorganic medium of sterilizing, inject high-purity methane and oxygen, make methane and the concentration of oxygen in culture tank head space be respectively 10% ~ 20%(v/v), 18% ~ 21% (v/v), individually carry out enlarged culturing step by step, obtain corresponding single bacterium liquid, namely respective methane-oxidizing bacteria seed liquor, will
methylosinus trichosporium,
methylosinus sporium,
methylocystissp.,
methylocystis parvusfour kinds of methane-oxidizing bacteria seed liquor are by bacterium number ratio (35 ~ 45): (3 ~ 10): (2 ~ 8): 1 mixing, obtains methane-oxidizing bacteria mixed bacteria liquid.
5. as claimed in claim 3 for purifying the preparation method of the composite fungus agent containing benzene homologues anaerobically fermenting waste gas, it is characterized in that, described benzene homologues degradation bacteria mixed bacteria liquid preparation method is:
Aseptically, will to have activated
azoarcus indigens, Terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. bacterium liquid is individually inoculated in the culture tank containing sterilized nitrate inorganic medium, after culture tank sealing, inject benzene homologues, make Benzene series Concentrations in headspace gas be 0.6-1.0%(v/v), in 30 ~ 35 ° of C, carry out enlarged culturing step by step under 100 ~ 150 rpm and obtain
azoarcus indigens, Terrimonassp.,
pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. respective seed liquor;
Will
azoarcus indigens,
terrimonassp.
, Pseudomonasaeruginosa,
bacillussp.,
ralstoniasp. and
runellasp. six kinds of seed liquor are by bacterium number ratio 1:(4 ~ 8): (4 ~ 8): (2 ~ 6): (2 ~ 6): (2 ~ 6) are mixed to get benzene homologues degradation germ liquid.
6. the preparation method for purifying the composite fungus agent containing benzene homologues anaerobically fermenting waste gas as described in claim arbitrary in claim 4 or 5, it is characterized in that, the formula of described nitrate inorganic medium is: NaNO
385 mg/L, Na
2sO
4142 mg/L, K
2hPO
43H
2o 1368 mg/L, KH
2pO
4h
2o 600.6 mg/L, CaCl
25.3 mg/L, FeSO
45H
2o 9.7 mg/L, ZnSO
47H
2o 0.6 mg/L, MgSO
47H
2o 36.9 mg/L, MnSO
4h
2o 0.44 mg/L, CuSO
46H
2o 0.27 mg/L, KI 0.17 mg/L, H
3bO
30.37 mg/L, CoCl
26H
2o 0.09 mg/L, NaMoO
42H
2o 0.09 mg/L, NaFeEDTA salt 3.8 mg/L, pH are 6 ~ 8; The incubation time of every one-level of enlarged culturing is 4 ~ 8 days step by step.
7. use the purposes of composite fungus agent as claimed in claim 1, it is characterized in that, for purifying methane in anaerobically fermenting waste gas and benzene homologues simultaneously, wherein benzene homologues is one or more in benzene, toluene, ethylbenzene and dimethylbenzene.
8. the purposes of composite fungus agent as claimed in claim 7, it is characterized in that, the concrete grammar that microbial inoculum is used for purifying methane in anaerobically fermenting waste gas and benzene homologues is simultaneously: pass in biological filter by the anaerobically fermenting waste gas containing methane and benzene homologues, and described composite fungus agent and nitrate inorganic medium are pressed the dilution proportion of 1:10 ~ 1:15, be sprayed at the surface of biological filter, methane and benzene homologues are degraded in biological filter.
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| CN109609411A (en) * | 2019-01-08 | 2019-04-12 | 北京科技大学 | A kind of microbial flora and preparation method thereof for erythromycin of degrading |
| CN111440754A (en) * | 2020-03-19 | 2020-07-24 | 南京农业大学 | Method for eliminating organic pollutant residues in soil by using genetically engineered methane-oxidizing bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109609411A (en) * | 2019-01-08 | 2019-04-12 | 北京科技大学 | A kind of microbial flora and preparation method thereof for erythromycin of degrading |
| CN111440754A (en) * | 2020-03-19 | 2020-07-24 | 南京农业大学 | Method for eliminating organic pollutant residues in soil by using genetically engineered methane-oxidizing bacteria |
| CN111440754B (en) * | 2020-03-19 | 2022-05-27 | 南京农业大学 | Method for eliminating organic pollutant residue in soil by using genetically engineered methane-oxidizing bacteria |
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