CN105012991B - Antibacterial anti hemorrhagic material with non-woven fibrous fabric structure and preparation method thereof - Google Patents
Antibacterial anti hemorrhagic material with non-woven fibrous fabric structure and preparation method thereof Download PDFInfo
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- CN105012991B CN105012991B CN201510425280.5A CN201510425280A CN105012991B CN 105012991 B CN105012991 B CN 105012991B CN 201510425280 A CN201510425280 A CN 201510425280A CN 105012991 B CN105012991 B CN 105012991B
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Abstract
A kind of antibacterial anti hemorrhagic material with non-woven fibrous fabric structure and preparation method thereof, belongs to biology medical material technical field.The antibacterial anti hemorrhagic material is characterized in that by aliphatic polyester dissolving electrospun fiber membrane is made in the technique for first passing through electrostatic spinning in a solvent, and on electrospun fiber membrane surface after loading chitosan, further loads fibrin ferment and form.Utilize the material characteristics of aliphatic polyester and chitosan, and the architectural feature such as high porosity high-specific surface area of the electrospun fiber membrane formed, steady load is carried out to fibrin ferment by the crosslinked action of chitosan and glutaraldehyde, forms the antibacterial anti hemorrhagic material with non-woven fibrous fabric structure.The material has good antibacterial and haemostatic effect, hence it is evident that better than commercially available gelfoam, and the advantages of be easy to remove with good mechanical performance, after easy to maintain, use, and preparation method is simple to operate, raw material usage saving.Product can be applied to the fields such as surgical operation, wound first aid.
Description
Technical field
The present invention relates to a kind of with electrospinning fibre antibacterial-hemostatic material of non-woven fibrous fabric structure and its preparation side
Method, belong to biological medical polymer material technical field.
Background technology
Hemostatic material and anti-biotic material have highly important status after wound in first aid or medical aid.At present clinically
Conventional hemostatic material has gelatin, oxycellulose, Fibrin Glue, chitosan etc., and form is often sponge, gauze etc., this
The anthemorrhagic performance especially anthemorrhagic speed of conventional hemostatic material can not still fully meet the demand of all emergency situations a bit.Blood coagulation
Enzyme has good haemostatic effect as a kind of biological agent, but commercially available is freeze-dried powder state, exist application method it is complicated and
The shortcomings of easy in inactivation.In addition, all kinds of anti-biotic materials also have substantial amounts of research report at present, wherein as natural macromolecular material
Chitosan not only has good biocompatibility and biodegradability, and has good antibacterial functions, is cured in biology
Had a wide range of applications with field.But its acidic aqueous solution has high viscosity, high electric charge and strong molecule due to chitosan
Interior and intermolecular hydrogen bonding effect, it is difficult to the spinning that individually succeeds.
Electrostatic spinning is that a kind of jet-action progress spinning using polymer solution or melt under forceful electric power field action adds
The technique of work.The fiber product for processing to obtain by electrostatic spinning is referred to as electrospinning fibre.The characteristics of electrospinning fibre maximum is that diameter is non-
Often small, several orders of magnitude smaller than fibre diameter made from conventional method, its diameter range is typically in 3nm~1 μm.Electrospinning fibre without
Spinning cloth porosity is high, specific surface area is big, fiber fine degree and homogeneity are high, draw ratio is big, and these are by conventional spinning method institute
The good characteristic that can not be obtained, impart the wide application prospect of electrospinning fibre.At present, in sensor, wound dressing, UF membrane
And the field such as organizational project, electrospinning fibre, which is obtained for, is widely applied research.
From material angle, aliphatic polyester has good biodegradability and good biocompatibility, has certain
Mechanical strength, compliance and spinnability, if aliphatic polyester can be prepared into electrospun fiber membrane through electrostatic spinning, and pass through
If the mode of surface coating chitosan is combined aliphatic polyester with the advantages of chitosan, then one is that can utilize its electrospinning fibre
Film has the architectural feature of very high porosity, makes it as the hemostatic material commonly used is if hemostatic gauze is as gelfoam,
The effect on complicated surface of a wound surface is easily adhered to by porous physical arrangement；Second, chitosan can be overcome to be not easy the difficulty of spinning
Point, by the use of chitosan as antibacterial material, make product that there is anti-microbial property.Third, chitosan stabilization, high-efficient carrier can be utilized
Haemostatic medicament fibrin ferment, realizes quick-acting haemostatic powder, product is can overcome the disadvantages that the blank in rapid hemostatic material.
Compared with the current existing hemostatic material containing fibrin ferment or clotting factor, the present invention has preferably hemostatic
Can, by the use of non-woven fibrous fabric structure as carrier, make product that there is more easily performance, moved after being easy to hemostasis
Remove.In addition, adding the storage stability of fibrin ferment using chitosan as stabilizer, the antibacterial-hemostasia products are made to be more easy to store.
And preparation method has simple to operate, raw material usage saving.
The content of the invention
It is an object of the invention to propose a kind of electrospinning fibre antibacterial-hemostatic material with non-woven fibrous fabric structure
And preparation method thereof, it is desirable to using the material characteristics of aliphatic polyester and chitosan, and the height of the electrospun fiber membrane formed
The architectural feature of porosity high-specific surface area etc., using chitosan and the crosslinked action steady load fibrin ferment of glutaraldehyde, improve
Fibrin ferment storage stability, material is set to reach application purpose and effect as antibacterial-hemostatic material.
Technical scheme is as follows：
A kind of antibacterial-hemostatic material with non-woven fibrous fabric structure, it is characterised in that the antibacterial-hemostatic material
In porous nonwoven fabric construct, contain aliphatic poly ester fiber, chitosan and fibrin ferment；Aliphatic polyester fiberoptic fiber is a diameter of
100nm~1500nm, porosity are 50%~90%, and static contact angle is 0 °~130 °, and material water absorbent rate is 2~20；
The material is prepared using following methods:Aliphatic polyester is made by fat using the method for electrostatic spinning first
Adoption ester fiber film, is then sufficiently impregnated in chitosan solution, makes the coated with nanometre yardstick of aliphatic poly ester fiber
Chitosan coat, then the aliphatic polyester tunica fibrosa of loading chitosan is completely infused in blood coagulation enzyme aqueous solution, dried
Obtain the antibacterial-hemostatic material with non-woven fibrous fabric structure afterwards.
A kind of preparation method of antibacterial-hemostatic material with non-woven fibrous fabric structure provided by the invention, it is special
Sign is that this method comprises the following steps：
1) spinning solution is made in aliphatic polyester dissolving in a solvent, then using electrospinning process by spinning solution
Aliphatic polyester electrospun fiber membrane is made；
2) dry aliphatic polyester electrospun fiber membrane is sufficiently impregnated in chitosan solution, takes out and done through room temperature
It is dry, obtain the aliphatic polyester electrospun fiber membrane of loading chitosan；
3) the aliphatic polyester electrospun fiber membrane of loading chitosan is completely infused in glutaraldehyde water solution, after taking-up
And through drying at room temperature, then be completely infused in blood coagulation enzyme aqueous solution, it is dried at room temperature for, that is, is obtained with nonwoven after taking-up
Antibacterial-hemostatic material of measuring fiber fabric construction.
In the method for the invention, preferably：The molecular weight 50000~500000 of chitosan described in step 2), take off second
Acyl degree >=80%, the chitosan solution are acidic aqueous solution, the mass percentage concentration of chitosan solution for 0.5wt%~
15wt%.The concentration of described blood coagulation enzyme aqueous solution is 0.5U/mL~10U/mL.The quality percentage of described glutaraldehyde water solution
Concentration is 0.0001wt%~1wt%.
In the above-mentioned technical proposal of the present invention, it is characterised in that described aliphatic polyester, refer to by having hydroxyl simultaneously
The polyester formed with the monomer of carboxyl through polycondensation, or polyester is formed through polycondensation by aliphatic dibasic acid and aliphatic dihydroxy alcohol, or
The polyester or copolyesters formed by aliphatic lactones through ring-opening polymerisation, aliphatic polyester molecular weight are 50000~250000.It is described
The polyester that there is the monomer of hydroxyl and carboxyl to be formed through polycondensation simultaneously is the PLA formed through acid through direct polycondensation by lactic；It is described by fat
The polyester that fat race binary acid and aliphatic dihydroxy alcohol form through condensation polymerization is poly butylene succinate, poly- decanedioic acid hexylene glycol
Ester, polyethylene glycol succinate or poly-succinic hexylene glycol ester；The polyester formed by aliphatic lactones through ring-opening polymerisation is by third
PLA that lactide ring-opening polymerisation forms, the polycaprolactone formed by caprolactone ring-opening polymerisation；Copolyesters is poly (glycolide-lactide).
Preferably, the solvent described in step 1) for dissolved fat adoption ester is hexafluoroisopropanol, tetrahydrofuran, N, N-
One or more of mixtures in dimethylformamide and trifluoroacetic acid；Formed spinning solution when mass percentage concentration be
5wt%~30wt%.Voltage in electrospinning process is 10KV~30KV, and spinning distance 10cm~20cm, injecting speed is
0.3mL/h~2mL/h, it is 100rpm~500rpm to receive rolling speed.
The present invention has advantages below and high-lighting effect：One kind proposed by the present invention has non-woven fibrous fabric structure
Antibacterial-hemostatic material, can fully with reference to raw material performance and electrospun fiber membrane architectural feature, reach antibacterial-hemostasis
Purpose.The antibacterial anti hemorrhagic material use chitosan and glutaraldehyde for the non-woven fibrous fabric structure that the present invention obtains are to fibrin ferment
The storage stability that steady load improves fibrin ferment is carried out, improves anthemorrhagic performance using fibrin ferment, hence it is evident that bright better than commercially available
Gelatin sponge.Product is more convenient using, storage and remove after being easy to hemostasis using non-woven fabrics as carrier.It is simultaneously of the present invention
The advantages of preparation method has simple to operate, is easy to produce in batches, and raw material usage is saved.
Brief description of the drawings
Fig. 1 is the scanning electricity of poly butylene succinate (PBS) nonwoven fabric construct that the present invention is prepared by embodiment 1
Mirror figure.
Fig. 2 is that poly butylene succinate (PBS) nonwoven fabric construct dip-coating shell that the present invention is prepared by embodiment 1 gathers
Scanning electron microscope (SEM) photograph after sugar.
Fig. 3 is that poly butylene succinate (PBS) nonwoven fabric construct dip-coating shell that the present invention is prepared by embodiment 1 gathers
Scanning electron microscope (SEM) photograph after sugar and fibrin ferment.
Embodiment
A kind of antibacterial-hemostatic material with non-woven fibrous fabric structure provided by the invention, in porous non-woven fabrics
Structure, contain aliphatic poly ester fiber, chitosan and fibrin ferment；A diameter of 100nm~the 1500nm of aliphatic polyester fiberoptic fiber,
Porosity is 50%~90%, and static contact angle is 0 °~130 °, and material water absorbent rate is 2~20；The material uses Static Spinning
Silk method carries out Electrospun to aliphatic polyester, and electrospun fiber membrane is made, and on electrospun fiber membrane surface after loading chitosan,
Further load fibrin ferment forms；Its specific preparation method is as follows：
Spinning solution is made in aliphatic polyester dissolving in a solvent first, then using the method for electrostatic spinning by spinning
Aliphatic polyester electrospun fiber membrane is made in solution；The species of described aliphatic poly ester solvent includes but is not limited to hexafluoro isopropyl
Alcohol, tetrahydrofuran, N,N-dimethylformamide or trifluoroacetic acid.Electrospinning conditions are unlimited but recommendation condition is：Form spinning
Mass percentage concentration during solution is 5wt%-30wt%, is voltage 10KV-30KV, spinning distance 10cm-20cm, injects speed
For 0.3mL/h-2Ml/h, it is 100rpm-500rpm to receive rolling speed.
The aliphatic polyester electrospun fiber membrane dried by more than, taken out and through room temperature after being sufficiently impregnated in chitosan solution
Dry, obtain the electrospun fiber membrane of loading chitosan；Deacetylation >=80% and molecular weight 50000- of described chitosan
500000, chitosan solution is generally acidic aqueous solution, but unlimited using the species of acid, when forming dipping chitosan solution
Mass percentage concentration is 0.5wt%-15wt%.
It is first complete using the glutaraldehyde water solution of debita spissitudo by the aliphatic polyester electrospun fiber membrane of loading chitosan
After dipping and through drying at room temperature, then using the blood coagulation enzyme aqueous solution thorough impregnation of debita spissitudo after, be dried at room temperature for, just obtain
Antibacterial-hemostatic material with non-woven fibrous fabric structure；The mass percentage concentration of described glutaraldehyde water solution is
0.0001wt%-1wt%；The concentration of described blood coagulation enzyme aqueous solution is 0.5U/mL-10U/mL.
Aliphatic polyester described in the preparation method, refer to by having the monomer of hydroxyl and carboxyl through polycondensation simultaneously and
Into, or formed through condensation polymerization by aliphatic dibasic acid and aliphatic dihydroxy alcohol, or formed by aliphatic lactones through ring-opening polymerisation
Polyester or copolyesters, its molecular weight are 50000-250000, wherein by having the monomer of hydroxyl and carboxyl to be formed through polycondensation simultaneously
Polyester include but is not limited to through acid through direct polycondensation by lactic into PLA (L-PLA, D-PLA)；By aliphatic dibasic acid and fat
The polyester that race's dihydric alcohol forms through condensation polymerization includes but is not limited to poly butylene succinate (PBS), poly- decanedioic acid hexylene glycol ester
(PHS), polyethylene glycol succinate (PES), poly-succinic hexylene glycol ester (PHS)；Formed by aliphatic lactones through ring-opening polymerisation
Polyester include but is not limited to the PLA (PLA) that is formed by lactide ring-opening polymerisation, formed by caprolactone ring-opening polymerisation poly-
Caprolactone (PCL)；Or copolyesters includes but is not limited to poly (glycolide-lactide) (PLGA).
The present invention will be further illustrated in following Examples：
Embodiment 1：The preparation of electrospun fiber membrane：9 grams of hexafluoroisopropanol is added in beaker, adds poly-succinic acid-butanediol
Ester (PBS) (molecular weight Mw=65000) 1g, at ambient temperature stirring make its dissolving, and it is 10wt% to obtain mass percentage concentration
Spinning solution；The spinning solution prepared obtains electrospun fiber membrane by electrostatic spinning, and prepared electrospun fiber membrane is existed
Desolvation 24h in vacuum drying oven.
Prepare chitosan solution：Glacial acetic acid dissolving is prepared into the acetum that concentration is 5wt% in deionized water, weighed
1.5g Chitosan powder (deacetylation>90%, molecular weight 200000) it is dissolved in the above-mentioned acetums of 100g and obtains quality
Fraction is 1.5wt% chitosan solution.
The electrospun fiber membrane of the certain area of clip is impregnated into above-mentioned chitosan solution, is soaked 30min, is taken out in room temperature bar
Dried under part, obtain the electrospun fiber membrane of loading chitosan.
The glutaraldehyde solution that mass fraction is 0.01% is prepared, the electrospun fiber membrane of the loading chitosan of above-mentioned preparation is soaked
Not in glutaraldehyde solution, 30min is soaked, is dried again under room temperature condition after taking-up.Fibrin ferment freeze-dried powder is taken to be matched somebody with somebody with deionized water
The thrombin solution that enzyme activity concentration is 2U/mL is made, above-mentioned electrospun fiber membrane is placed in thrombin solution and soaks 30min, is taken
Go out it is rearmounted dry at room temperature, obtain the antibacterial-hemostatic material with non-woven fibrous fabric structure.
Anthemorrhagic performance：It is bright with market product using the anthemorrhagic performance of rabbit liver bleeding due to trauma scale-model investigation electrospun fiber membrane
Gelatin sponge compares, and electrospun fiber membrane bleeding stopping period is shorter than gelfoam.
Anti-microbial property：Prepared electrospun fiber membrane is possessed into antibacterial ability, according to following characterizing method measure for
E.coli (DH5 α) 2h contact sterilization rates reach 80%.
Antibacterial assay method：
LB culture mediums：1g NaCl, 1g tryptones (tryptone), 0.5g yeast are added in 100mL deionized waters
Extract (yeast extract), it is distributed into 50mL conical flasks after stirring and dissolving, every bottle of 5mL, in high-pressure steam sterilizing pan
120 DEG C of sterilizing 15min.
LB- agar mediums (LB flat boards)：1g NaCl, 1g tryptones are added in 100mL deionized waters
(tryptone), 0.5g yeast extracts (yeast extract), 2g agar (agar) is added after stirring and dissolving, is steamed in high pressure
120 DEG C of sterilizing 15min in vapour autoclave.Sterilized culture medium is distributed into the 9cm through high pressure steam sterilization before cooling and solidifying
In culture dish, each culture dish adds about 20mL culture mediums, is advisable so that culture dish is completely covered, is cooled and solidified in super-clean bench.
Antibacterial ability test is carried out using Gram-E. coli (E.coli, DH5 α).
Bacterium preculture：Strain is accessed in LB culture mediums, at 37 DEG C, to be incubated in the shaking table of 230rpm velocity fluctuation
18h is educated, it is standby.The dense about 109cfu/mL of bacterium.
Anti-microbial property determination experiment is determined using contact sterilization method.Testing sample and reference sample are cut into 1cm × 1cm
Size, after 30min sterilizings are handled in 75% ethanol, dried in super-clean bench.Sterilize and dried sample is positioned over LB
On flat board, it is ensured that it is bonded completely with media surface.The good bacterium solution of 10 μ L precultures is inoculated with each sample, in 37 DEG C of cultures
After being incubated 2h in case, sample is removed, is put into and is previously added in the test tube of 1mL deionized waters, sustained vibration 5min, by sample
Bacterium elute completely, obtain 100 times of bacterial suspension of dilution.Bacterial suspension is through gradient dilution to 102、103、104、105、106、
107After times, 100 μ L are respectively taken, are coated on LB flat boards, the bacterium measured using colony counting method on each sample is dense.Test sample
On bacterium dense be designated as A1(cfu/mL) bacterium, in corresponding reference sample is dense to be designated as A0(cfu/mL), then sterilizing rate P passes through following formula meter
Obtain：
Embodiment 2：The molecular weight of poly butylene succinate in embodiment 1 is changed to Mw=100000, preparation method is same
Embodiment 1, the bleeding stopping period of electrospun fiber membrane are shorter than gelfoam.Reach for E.coli (DH5 α) 2h contact sterilization rates
83%.
Embodiment 3：Poly butylene succinate in embodiment 1 is changed to polycaprolactone, usage amount 0.6g, preparation method
5U/mL is changed to embodiment 1, but by thrombin solution concentration, the bleeding stopping period of electrospun fiber membrane is shorter than gelfoam.For
E.coli (DH5 α) 2h contact sterilization rates reach 72%.
Embodiment 4：Poly butylene succinate in embodiment 1 is changed to PLA, usage amount 0.6g, preparation method is same
Embodiment 1, the bleeding stopping period of electrospun fiber membrane are shorter than gelfoam.Reach for E.coli (DH5 α) 2h contact sterilization rates
68%.
Embodiment 5：Poly butylene succinate in embodiment 1 is changed to the poly- decanedioic acid hexylene glycol ester of same amount, solvent changes
For trifluoroacetic acid, preparation method is shorter than gelfoam with embodiment 1, the bleeding stopping period of electrospun fiber membrane.For E.coli (DH5
2h contact sterilization rates α) reach 69%.
Embodiment 6：Poly butylene succinate in embodiment 1 is changed to the poly (glycolide-lactide) of same amount, chitosan (de- second
Acyl degree is 80%, molecular weight 300000), compound concentration is 2wt% solution, and other preparation methods are fine with embodiment 1, electrospinning
The bleeding stopping period of dimension film is shorter than gelfoam.Reach 90% for E.coli (DH5 α) 2h contact sterilization rates.
Embodiment 7：The fixed amount of fibrin ferment in embodiment 5 is changed to 5U/mL, preparation method is the same as embodiment 5, electrospinning fibre
The bleeding stopping period of film is shorter than gelfoam.Reach 92% for E.coli (DH5 α) 2h contact sterilization rates.
Embodiment 8：Preparation method is with embodiment 4, and the concentration for changing glutaraldehyde solution is 0.1%, and other preparation methods are same
Embodiment 4, the bleeding stopping period of electrospun fiber membrane are shorter than gelfoam.Reach for E.coli (DH5 α) 2h contact sterilization rates
72%.
Embodiment 9：The mass concentration of solute in embodiment 1 is changed to 15%, preparation method is the same as embodiment 1, electrospinning fibre
The bleeding stopping period of film is shorter than gelfoam.Reach 82% for E.coli (DH5 α) 2h contact sterilization rates.
Embodiment 10：The acetum of 5wt% in embodiment 6 is changed to 1wt% aqueous hydrochloric acid solution, other preparation sides
Method is shorter than gelfoam with embodiment 6, the bleeding stopping period of electrospun fiber membrane.Reached for E.coli (DH5 α) 2h contact sterilization rates
To 90%.
Comparative example 1：Directly rabbit liver surface damage Hemorrhage Model is stopped blooding using hospital gauze, bleeding stopping period is significantly
It is longer than gained electrospinning fibre sample.It is invalid for E.coli (DH5 α) 2h contact sterilizations.
Comparative example 2：Rabbit liver surface damage Hemorrhage Model is stopped blooding using gelfoam, bleeding stopping period is longer than gained
Electrospinning fibre sample.It is invalid for E.coli (DH5 α) 2h contact sterilizations.
The present invention can be summarized with others without prejudice to the concrete form of the spirit or essential characteristics of the present invention.Therefore, nothing
By from which
From the point of view of a bit, the embodiment above of the invention can only all be considered the description of the invention and can not limit this hair
It is bright.
Claims (10)
1. a kind of antibacterial-hemostatic material with non-woven fibrous fabric structure, it is characterised in that the antibacterial-hemostatic material is in
Porous nonwoven fabric construct, contain aliphatic poly ester fiber, chitosan and fibrin ferment；Aliphatic polyester fiberoptic fiber is a diameter of
100nm~1500nm, porosity are 50%~90%, and static contact angle is 0 °~130 °, and material water absorbent rate is 2~20；
The material is prepared using following methods：Aliphatic polyester is made by aliphatic poly using the method for electrostatic spinning first
Ester fiber film, is then sufficiently impregnated in chitosan solution, gathers the shell of the coated with nanometre yardstick of aliphatic poly ester fiber
Sugared coating, then the aliphatic polyester tunica fibrosa of loading chitosan is completely infused in blood coagulation enzyme aqueous solution, after drying i.e.
Obtain the antibacterial-hemostatic material with non-woven fibrous fabric structure.
2. a kind of preparation method of antibacterial-hemostatic material with non-woven fibrous fabric structure as claimed in claim 1, it is special
Sign is that this method comprises the following steps：
1) spinning solution is made in aliphatic polyester dissolving in a solvent, then spinning solution is made using electrospinning process
Aliphatic polyester electrospun fiber membrane；
2) dry aliphatic polyester electrospun fiber membrane is sufficiently impregnated in chitosan solution, takes out and through drying at room temperature, obtain
To the aliphatic polyester electrospun fiber membrane of loading chitosan；
3) the aliphatic polyester electrospun fiber membrane of loading chitosan is completely infused in glutaraldehyde water solution, after taking-up and passed through
Drying at room temperature, then be completely infused in blood coagulation enzyme aqueous solution, it is dried at room temperature for, that is, obtains fine with non-woven fabrics after taking-up
Antibacterial-hemostatic material of dimensional fabric structure.
3. according to a kind of preparation method of antibacterial-hemostatic material with non-woven fibrous fabric structure described in claim 2,
Characterized in that, the molecular weight 50000~500000 of the chitosan described in step 2), deacetylation >=80%, chitosan are molten
Liquid is acidic aqueous solution, and the mass percentage concentration of chitosan solution is 0.5wt%~15wt%.
4. according to a kind of preparation method of antibacterial-hemostatic material with non-woven fibrous fabric structure described in claim 2,
Characterized in that, the concentration of described blood coagulation enzyme aqueous solution is 0.5U/mL~10U/mL.
5. according to a kind of preparation method of antibacterial-hemostatic material with non-woven fibrous fabric structure described in claim 2,
Characterized in that, the mass percentage concentration of described glutaraldehyde water solution is 0.0001wt%~1wt%.
6. according to a kind of system of antibacterial-hemostatic material with non-woven fibrous fabric structure described in claim 2,3,4 or 5
Preparation Method, it is characterised in that described aliphatic polyester, refer to what is formed by having the monomer of hydroxyl and carboxyl simultaneously through polycondensation
Polyester, or polyester is formed through polycondensation by aliphatic dibasic acid and aliphatic dihydroxy alcohol, or by aliphatic lactones through ring-opening polymerisation and
Into polyester or copolyesters, aliphatic polyester molecular weight be 50000~250000.
7. according to a kind of preparation method of antibacterial-hemostatic material with non-woven fibrous fabric structure described in claim 6,
Characterized in that, polyester described while that there is the monomer of hydroxyl and carboxyl to be formed through polycondensation forms through acid through direct polycondensation by lactic
PLA；The polyester formed by aliphatic dibasic acid and aliphatic dihydroxy alcohol through condensation polymerization be poly butylene succinate,
Poly- decanedioic acid hexylene glycol ester, polyethylene glycol succinate or poly-succinic hexylene glycol ester；By aliphatic lactones through ring-opening polymerisation and
Into polyester be the PLA, the polycaprolactone that is formed by caprolactone ring-opening polymerisation that are formed by lactide ring-opening polymerisation；Copolyesters
For poly (glycolide-lactide).
8. according to a kind of system of antibacterial-hemostatic material with non-woven fibrous fabric structure described in claim 2,3,4 or 5
Preparation Method, it is characterised in that the solvent that step 1) is used for dissolved fat adoption ester is hexafluoroisopropanol, tetrahydrofuran, N, N- bis-
One or more of mixtures in NMF and trifluoroacetic acid.
9. according to a kind of system of antibacterial-hemostatic material with non-woven fibrous fabric structure described in claim 2,3,4 or 5
Preparation Method, it is characterised in that mass percentage concentration when spinning solution is formed in step 1) is 5wt%~30wt%.
10. according to a kind of antibacterial-hemostatic material with non-woven fibrous fabric structure described in claim 2,3,4 or 5
Preparation method, it is characterised in that the voltage in electrospinning process is 10KV~30KV, spinning distance 10cm~20cm, is injected
Speed is 0.3mL/h~2mL/h, and it is 100rpm~500rpm to receive rolling speed.
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| CN201510425280.5A CN105012991B (en) | 2015-07-17 | 2015-07-17 | Antibacterial anti hemorrhagic material with non-woven fibrous fabric structure and preparation method thereof |
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