CN108273117A - A kind of preparation method of Medical absorbable hemostatic material - Google Patents
A kind of preparation method of Medical absorbable hemostatic material Download PDFInfo
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- CN108273117A CN108273117A CN201810166769.9A CN201810166769A CN108273117A CN 108273117 A CN108273117 A CN 108273117A CN 201810166769 A CN201810166769 A CN 201810166769A CN 108273117 A CN108273117 A CN 108273117A
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Abstract
The present invention provides a kind of preparation methods of Medical absorbable hemostatic material.Preparation method is as follows：（1）Spinning solution is prepared with polylactic acid, cellulose acetate, the compound porous superfine fibre film of polylactic acid/acetate fiber is made by electrostatic spinning；（2）Tunica fibrosa is impregnated with NaOH；（3）Tunica fibrosa EEDC and Sulfo NHS mixed solutions are set again to impregnate；（4）After clean film is placed in hyaluronic acid solution reaction, drying for standby；（5）Prepare the mixed solution that modified composite membrane impregnates；（6）By the tunica fibrosa of modification in step（5）Hot pressing after being impregnated in solution obtained, obtains Medical absorbable hemostatic material.The Medical absorbable hemostatic material water absorbing properties of the present invention are good, and hemostasis is fast.
Description
Technical field
The present invention relates to field of medical materials, and in particular to a kind of Medical absorbable hemostatic material and preparation method thereof.
Background technology
Electrostatic spinning technique, which is one kind, can continuously, quickly prepare a diameter of 10 μm of -10nm fibers.With traditional spinning fibre
Dimension is compared, and electrospinning fibre has high-specific surface area, and there is also nanoscale hole holes on many fibers, and porosity is very high, because
This filtering material, tissue engineering bracket material field show the foreground of being widely applied.Especially three-dimensional porous material is conducive to
Nutriment transports transmission wherein, thus it is particularly suited for biological tissue's branch than the fiber of nonporous fiber or only surface holes
Frame material or drug release material.Therefore the research of electrospinning fibre and its membrane material with three-dimensional porous structure causes in recent years
The extensive concern of people.Polylactic acid has good biocompatibility, high osmosis, good mechanical performance and biodegradable
Property, therefore polylactic acid is with a wide range of applications in tissue engineering bracket field.But polylactic acid membrane is more crisp, passes through at present
The method of many blendings improves its mechanical performance.Cellulose acetate is the derivative of natural polymer cellulose, is a kind of tool
There is the biomaterial of good biocompatibility and biological degradability.As a kind of aliphatic poly ester fiber, acid fiber by polylactic is hydrophilic
Property it is poor, it is therefore desirable to improve hydrophily by modified.Drug can be preferably transmitted using the porous structure of fiber, makes its hair
Wave best effect.
Invention content
Technical problems to be solved：The object of the present invention is to provide a kind of Medical absorbable hemostatic materials, pass through Static Spinning
Technology makes film have porous structure, is suitble to the transmission of drug, while the material must have good water absorbing properties.
Technical solution：A kind of preparation method of Medical absorbable hemostatic material, includes the following steps：
（1）It is 5 that polylactic acid, cellulose acetate, which are dissolved in volume ratio,:1-2:1 dichloromethane and n,N-Dimethylformamide
In mixed solution, it is 10% to be configured to mass concentration, and 4-10h is stirred under room temperature, is gathered by the way that electrostatic spinning is obtained after obtaining spinning solution
The compound porous superfine fibre film of lactic acid/acetate fiber is dried in vacuo 12h in 60-90 DEG C, spare；
（2）Tunica fibrosa is initially positioned in the NaOH solution of 0.1wt%, ultrasonic shear 30-60min uses PBS solution after taking-up
10min is impregnated, then wash with distilled water 3 times；
（3）Tunica fibrosa is placed in the EDS solution of 0.01-0.5wt% again, Sulfo-NHS is pressed into nEDC：NSulfo-NHS is 5:1
Amount be added, placed at 4 DEG C for 24 hours, then with PBS solution impregnate 1h after, 3 times wash with distilled water；
（4）Clean film is placed in 0.01-0.2wt% hyaluronic acid solutions, after aspirating vacuum, 10- is preserved in 4 DEG C of refrigerators
Centrifugation 2-10min, rotating speed 1000-2000r/min are taken out after 30 h, then after impregnating 1h with PBS solution, wash with distilled water 3
It is secondary, drying for standby under room temperature；
（5）0.8-2.5 parts of native fragile blueberry root leaf extract, 0.5-3.2 parts of Orbicular Lindsaea extract, Wa Wei are weighed by weight
0.5-1.6 parts of extract, 0.5-1.2 parts of canarium pimela extract, 1-3 parts of glycine, -3 parts of arginase 12, sulfated chitosan 1-5
Part, 1.5-2.5 parts of glutathione, 40-60 parts of medical distilled water, are configured to aqueous solution, are stirred at room temperature uniformly；
（6）By the tunica fibrosa of modification in step（5）2-4h is impregnated in solution obtained, then freeze-drying exists to being dehydrated
Hot pressing 20min in 35-45 DEG C of heating platen obtains the Medical absorbable hemostatic material.
Further, the preparation method of a kind of Medical absorbable hemostatic material, the step（1）Middle dichloromethane
Volume ratio with n,N-Dimethylformamide is 4:1, mixing time 5-8h, drying temperature are 70-80 DEG C.
Further, the preparation method of a kind of Medical absorbable hemostatic material, the step（2）Middle ultrasonic wave is cut
The time cut is 50min.
Further, the preparation method of a kind of Medical absorbable hemostatic material, the step（3）Middle EDS concentration
For 0.1wt%.
Further, the preparation method of a kind of Medical absorbable hemostatic material, the step（4）Hyaluronic acid is molten
A concentration of 0.05-0.15wt% of liquid, holding time 15-25h, centrifugation time 5min, rotating speed 1200-1600r/min.
Further, the preparation method of a kind of Medical absorbable hemostatic material, the step（5）In extract
It is extracted by 50-75wt% ethyl alcohol.
Further, the preparation method of a kind of Medical absorbable hemostatic material, the step（5）Raw material is by as follows
Parts by weight weigh, 1.2-2 parts of fragile blueberry root leaf extract, 1.5-2.5 parts of Orbicular Lindsaea extract, watt Wei extract 0.8-
0.4 part, 0.6-1 parts of canarium pimela extract, 1.5-2.5 parts of glycine, -2.6 parts of arginase 12,2-4 parts of sulfated chitosan, paddy Guang
Sweet peptide 1.8-2.2 parts, 45-55 parts of medical distilled water.
Further, the preparation method of a kind of Medical absorbable hemostatic material, the step（6）Middle soaking time
For 3h, hot pressing plate temperature is 40 DEG C.
Advantageous effect：The perforated membrane that is prepared by electrostatic spinning of Medical absorbable hemostatic material of the present invention, using changing
Property after there is very high water absorption rate, the water absorption rate of the hemostatic material to be 668-758%, on the basis of porous and modified, film is soaking
Contain Chinese medicine hemostatic compositions after bubble, the more advantageous hemostatic material plays the effect of hemostasis, and the clotting index of the material is 16.3-
23.4。
Specific implementation mode
Embodiment 1
A kind of preparation method of Medical absorbable hemostatic material, includes the following steps：（1）Polylactic acid, cellulose acetate are dissolved
It is 2 in volume ratio:In 1 dichloromethane and n,N-Dimethylformamide mixed solution, it is 10% to be configured to mass concentration, room temperature
Lower stirring 10h is obtained and the compound porous superfine fibre film of polylactic acid/acetate fiber is made by electrostatic spinning after spinning solution, in 90
DEG C vacuum drying 12h, it is spare；（2）Tunica fibrosa is initially positioned in the NaOH solution of 0.1wt%, ultrasonic shear 30min, is taken out
10min is impregnated with PBS solution afterwards, then wash with distilled water 3 times；（3）Tunica fibrosa is placed in the EDS solution of 0.5wt% again,
Sulfo-NHS is pressed into nEDC：NSulfo-NHS is 5:1 amount is added, and is placed at 4 DEG C for 24 hours, then after impregnating 1h with PBS solution,
3 times wash with distilled water；（4）Clean film is placed in 0.01wt% hyaluronic acid solutions, after aspirating vacuum, in 4 DEG C of refrigerators
Centrifugation 10min, rotating speed 2000r/min are taken out after 10 h of middle preservation, then after impregnating 1h with PBS solution, wash with distilled water 3
It is secondary, drying for standby under room temperature；（5）0.8 part of native fragile blueberry root leaf extract, Orbicular Lindsaea extract 3.2 are weighed by weight
0.5 part of part, watt Wei extract, 1.2 parts of canarium pimela extract, 3 parts of glycine, arginase 12 part, 1 part of sulfated chitosan, gluathione
1.5 parts of peptide, 40 parts of medical distilled water, are configured to aqueous solution, are stirred at room temperature uniformly；（6）By the tunica fibrosa of modification in step
（5）2h is impregnated in solution obtained, freeze-drying is to being dehydrated, and then the hot pressing 20min in 45 DEG C of heating platen, obtains the doctor
Use Absorbable hemostatic material.
Embodiment 2
A kind of preparation method of Medical absorbable hemostatic material, includes the following steps：（1）Polylactic acid, cellulose acetate are dissolved
It is 5 in volume ratio:In 1 dichloromethane and n,N-Dimethylformamide mixed solution, it is 10% to be configured to mass concentration, room temperature
Lower stirring 4h is obtained and the compound porous superfine fibre film of polylactic acid/acetate fiber is made by electrostatic spinning after spinning solution, in 60 DEG C
It is dried in vacuo 12h, it is spare；（2）Tunica fibrosa is initially positioned in the NaOH solution of 0.1wt%, ultrasonic shear 60min, after taking-up
10min is impregnated with PBS solution, then wash with distilled water 3 times；（3）Tunica fibrosa is placed in the EDS solution of 0.01wt% again, it will
Sulfo-NHS presses nEDC：NSulfo-NHS is 5:1 amount is added, and is placed at 4 DEG C for 24 hours, then with after PBS solution immersion 1h, uses
Distilled water cleans 3 times；（4）Clean film is placed in 0.2wt% hyaluronic acid solutions, after aspirating vacuum, is protected in 4 DEG C of refrigerators
It deposits and takes out centrifugation 2min, rotating speed 1000r/min after 30 h, then after impregnating 1h with PBS solution, 3 times wash with distilled water, room temperature
Lower drying for standby；（5）2.5 parts of native fragile blueberry root leaf extract, 0.5 part of Orbicular Lindsaea extract, Wa Wei are weighed by weight
1.6 parts of extract, 0.5 part of canarium pimela extract, 1 part of glycine, 3 parts of arginine, 5 parts of sulfated chitosan, 2.5 parts of gluathione,
60 parts of medical distilled water, is configured to aqueous solution, is stirred at room temperature uniformly；（6）By the tunica fibrosa of modification in step（5）It is obtained
4h is impregnated in solution, freeze-drying is to being dehydrated, and then the hot pressing 20min in 35 DEG C of heating platen, obtains the Medical absorbable
Hemostatic material.
Embodiment 3
A kind of preparation method of Medical absorbable hemostatic material, includes the following steps：（1）Polylactic acid, cellulose acetate are dissolved
It is 4 in volume ratio:In 1 dichloromethane and n,N-Dimethylformamide mixed solution, it is 10% to be configured to mass concentration, room temperature
Lower stirring 5h is obtained and the compound porous superfine fibre film of polylactic acid/acetate fiber is made by electrostatic spinning after spinning solution, in 70 DEG C
It is dried in vacuo 12h, it is spare；（2）Tunica fibrosa is initially positioned in the NaOH solution of 0.1wt%, ultrasonic shear 50min, after taking-up
10min is impregnated with PBS solution, then wash with distilled water 3 times；（3）Tunica fibrosa is placed in the EDS solution of 0.1wt% again, it will
Sulfo-NHS presses nEDC：NSulfo-NHS is 5:1 amount is added, and is placed at 4 DEG C for 24 hours, then with after PBS solution immersion 1h, uses
Distilled water cleans 3 times；（4）Clean film is placed in 0.05wt% hyaluronic acid solutions, after aspirating vacuum, in 4 DEG C of refrigerators
It preserves and takes out centrifugation 5min, rotating speed 1200r/min after 15 h, then after impregnating 1h with PBS solution, 3 times wash with distilled water, often
The lower drying for standby of temperature；（5）1.2 parts of fragile blueberry root leaf extract, 2.5 parts of Orbicular Lindsaea extract, Wa Wei are weighed by weight
0.8 part of extract, 0.6 part of canarium pimela extract, 1.5 parts of glycine .6 parts of arginase 12,4 parts of sulfated chitosan, glutathione
1.8 parts, 45 parts of medical distilled water, are configured to aqueous solution, are stirred at room temperature uniformly；（6）By the tunica fibrosa of modification in step（5）
3h is impregnated in solution obtained, freeze-drying is to being dehydrated, and then the hot pressing 20min in 40 DEG C of heating platen, obtains described medical
Absorbable hemostatic material.
Embodiment 4
A kind of preparation method of Medical absorbable hemostatic material, includes the following steps：（1）Polylactic acid, cellulose acetate are dissolved
It is 4 in volume ratio:In 1 dichloromethane and n,N-Dimethylformamide mixed solution, it is 10% to be configured to mass concentration, room temperature
Lower stirring 8h is obtained and the compound porous superfine fibre film of polylactic acid/acetate fiber is made by electrostatic spinning after spinning solution, in 80 DEG C
It is dried in vacuo 12h, it is spare；（2）Tunica fibrosa is initially positioned in the NaOH solution of 0.1wt%, ultrasonic shear 50min, after taking-up
10min is impregnated with PBS solution, then wash with distilled water 3 times；（3）Tunica fibrosa is placed in the EDS solution of 0.1wt% again, it will
Sulfo-NHS presses nEDC：NSulfo-NHS is 5:1 amount is added, and is placed at 4 DEG C for 24 hours, then with after PBS solution immersion 1h, uses
Distilled water cleans 3 times；（4）Clean film is placed in 0.15wt% hyaluronic acid solutions, after aspirating vacuum, in 4 DEG C of refrigerators
It preserves and takes out centrifugation 5min, rotating speed 1600r/min after 25 h, then after impregnating 1h with PBS solution, 3 times wash with distilled water, often
The lower drying for standby of temperature；（5）2 parts of fragile blueberry root leaf extract, 1.5 parts of Orbicular Lindsaea extract, Wa Wei is weighed by weight to carry
Take 0.4 part of object, 1 part of canarium pimela extract, 2.5 parts of glycine, arginase 12 part, 2 parts of sulfated chitosan, 2.2 parts of glutathione,
55 parts of medical distilled water, is configured to aqueous solution, is stirred at room temperature uniformly；（6）By the tunica fibrosa of modification in step（5）It is obtained
3h is impregnated in solution, freeze-drying is to being dehydrated, and then the hot pressing 20min in 40 DEG C of heating platen, obtains the Medical absorbable
Hemostatic material.
Embodiment 5
A kind of preparation method of Medical absorbable hemostatic material, includes the following steps：（1）Polylactic acid, cellulose acetate are dissolved
It is 4 in volume ratio:In 1 dichloromethane and n,N-Dimethylformamide mixed solution, it is 10% to be configured to mass concentration, room temperature
Lower stirring 6h is obtained and the compound porous superfine fibre film of polylactic acid/acetate fiber is made by electrostatic spinning after spinning solution, in 75 DEG C
It is dried in vacuo 12h, it is spare；（2）Tunica fibrosa is initially positioned in the NaOH solution of 0.1wt%, ultrasonic shear 50min, after taking-up
10min is impregnated with PBS solution, then wash with distilled water 3 times；（3）Tunica fibrosa is placed in the EDS solution of 0.1wt% again, it will
Sulfo-NHS presses nEDC：NSulfo-NHS is 5:1 amount is added, and is placed at 4 DEG C for 24 hours, then with after PBS solution immersion 1h, uses
Distilled water cleans 3 times；（4）Clean film is placed in 0.1wt% hyaluronic acid solutions, after aspirating vacuum, is protected in 4 DEG C of refrigerators
It deposits and takes out centrifugation 5min, rotating speed 1400r/min after 20h, then after impregnating 1h with PBS solution, 3 times wash with distilled water, room temperature
Lower drying for standby；（5）1.6 parts of fragile blueberry root leaf extract, the extraction of 2 part, watt Wei of Orbicular Lindsaea extract are weighed by weight
0.7 part of object, 0.8 part of canarium pimela extract, 2 parts of glycine .4 parts of arginase 12,3 parts of sulfated chitosan, 2 parts of glutathione, doctor
With 50 parts of distilled water, it is configured to aqueous solution, is stirred at room temperature uniformly；（6）By the tunica fibrosa of modification in step（5）It is obtained molten
3h is impregnated in liquid, freeze-drying is to being dehydrated, and then the hot pressing 20min in 40 DEG C of heating platen, obtains the Medical absorbable and stop
Blood material.
Comparative example 1
A kind of preparation method of Medical absorbable hemostatic material, includes the following steps：（1）Polylactic acid, cellulose acetate are dissolved
It is 2 in volume ratio:In 1 dichloromethane and n,N-Dimethylformamide mixed solution, it is 10% to be configured to mass concentration, room temperature
Lower stirring 10h is obtained and the compound porous superfine fibre film of polylactic acid/acetate fiber is made by electrostatic spinning after spinning solution, in 90
DEG C vacuum drying 12h, it is spare；（2）Tunica fibrosa is initially positioned in the NaOH solution of 0.1wt%, ultrasonic shear 30min, is taken out
10min is impregnated with PBS solution afterwards, then wash with distilled water 3 times；（3）Native fragile blueberry root leaf extract 0.8 is weighed by weight
Part, 3.2 0.5 part of part, watt Wei extract of Orbicular Lindsaea extract, 1.2 parts of canarium pimela extract, 3 parts of glycine, arginase 12 part,
1 part of sulfated chitosan, 1.5 parts of glutathione, 40 parts of medical distilled water, are configured to aqueous solution, are stirred at room temperature uniformly；（4）It will
Tunica fibrosa is in step（3）2h, freeze-drying to dehydration, the then hot pressing in 45 DEG C of heating platen are impregnated in solution obtained
20min obtains the Medical absorbable hemostatic material.
Comparative example 2
A kind of preparation method of Medical absorbable hemostatic material, includes the following steps：（1）Polylactic acid, cellulose acetate are dissolved
It is 5 in volume ratio:In 1 dichloromethane and n,N-Dimethylformamide mixed solution, it is 10% to be configured to mass concentration, room temperature
Lower stirring 4h is obtained and the compound porous superfine fibre film of polylactic acid/acetate fiber is made by electrostatic spinning after spinning solution, in 60 DEG C
It is dried in vacuo 12h, it is spare；（2）Tunica fibrosa is initially positioned in the NaOH solution of 0.1wt%, ultrasonic shear 60min, after taking-up
10min is impregnated with PBS solution, then wash with distilled water 3 times；（3）Tunica fibrosa is placed in the EDS solution of 0.01wt% again, it will
Sulfo-NHS presses nEDC：NSulfo-NHS is 5:1 amount is added, and is placed at 4 DEG C for 24 hours, then with after PBS solution immersion 1h, uses
Distilled water cleans 3 times；（4）Clean film is placed in 0.2wt% hyaluronic acid solutions, after aspirating vacuum, is protected in 4 DEG C of refrigerators
It deposits and takes out centrifugation 2min, rotating speed 1000r/min after 30 h, then after impregnating 1h with PBS solution, 3 times wash with distilled water, room temperature
Lower drying for standby；（5）1 part of glycine, 3 parts of arginine, 5 parts of sulfated chitosan, 2.5 parts of gluathione, doctor are weighed by weight
With 60 parts of distilled water, it is configured to aqueous solution, is stirred at room temperature uniformly；（6）By the tunica fibrosa of modification in step（5）It is obtained molten
4h is impregnated in liquid, freeze-drying is to being dehydrated, and then the hot pressing 20min in 35 DEG C of heating platen, obtains the Medical absorbable and stop
Blood material.
Water absorption rate test：The water absorption rate for changing material is measured using mass method, is weighed the hemostatic material of certain mass (m1), is put
Enter in distilled water and impregnate, surface moisture then is wiped into hemostatic material taking-up after impregnating 10 min respectively, claims its quality (m2
), water absorption rate is calculated as follows：ε=[(m2-m1)/m1] × 100%.
Clotting index is tested：It extracts rabbit new blood and to be added appropriate anti-coagulants for use, which is cut into 0.5cm2
It lies in beaker, is put into constant temperature 5min in 37 DEG C of water-bath, the blood of 0.1mL is added drop-wise on sample, is dripped immediately after
Add 0.02mLCaCl2Solution starts coagulation process, and 25mL 37 DEG C of constant temperature of deionized water, which are added, after 5min waves 5min, then removes
Solution surveys its Abs value in wavelength with ultraviolet specrophotometer at 415nm.After 0.1mL blood is diluted 25mL with deionized water
Abs values make reference value, clotting index is calculated as follows：BCI=100 × Abs samples/Abs references.
The property indices of material of the present invention see the table below, it may be seen that this Medical absorbable hemostatic material pass through it is quiet
Perforated membrane prepared by Electrospun has very high water absorption rate using modified, and the water absorption rate of the hemostatic material is 668-758%,
On the basis of porous and modified, film contains Chinese medicine hemostatic compositions after steeping, and the more advantageous hemostatic material plays the work of hemostasis
With the clotting index of the material is 16.3-23.4.
The property indices of 1 Medical absorbable hemostatic material of table
Claims (8)
1. a kind of preparation method of Medical absorbable hemostatic material, which is characterized in that include the following steps：
（1）It is 5 that polylactic acid, cellulose acetate, which are dissolved in volume ratio,:1-2:1 dichloromethane and n,N-Dimethylformamide
In mixed solution, it is 10% to be configured to mass concentration, and 4-10h is stirred under room temperature, is gathered by the way that electrostatic spinning is obtained after obtaining spinning solution
The compound porous superfine fibre film of lactic acid/acetate fiber is dried in vacuo 12h in 60-90 DEG C, spare；
（2）Tunica fibrosa is initially positioned in the NaOH solution of 0.1wt%, ultrasonic shear 30-60min uses PBS solution after taking-up
10min is impregnated, then wash with distilled water 3 times；
（3）Tunica fibrosa is placed in the EDS solution of 0.01-0.5wt% again, Sulfo-NHS is pressed into nEDC：NSulfo-NHS is 5:1
Amount be added, placed at 4 DEG C for 24 hours, then with PBS solution impregnate 1h after, 3 times wash with distilled water；
（4）Clean film is placed in 0.01-0.2wt% hyaluronic acid solutions, after aspirating vacuum, 10- is preserved in 4 DEG C of refrigerators
Centrifugation 2-10min, rotating speed 1000-2000r/min are taken out after 30 h, then after impregnating 1h with PBS solution, wash with distilled water 3
It is secondary, drying for standby under room temperature；
（5）0.8-2.5 parts of native fragile blueberry root leaf extract, 0.5-3.2 parts of Orbicular Lindsaea extract, Wa Wei are weighed by weight
0.5-1.6 parts of extract, 0.5-1.2 parts of canarium pimela extract, 1-3 parts of glycine, -3 parts of arginase 12, sulfated chitosan 1-5
Part, 1.5-2.5 parts of glutathione, 40-60 parts of medical distilled water, are configured to aqueous solution, are stirred at room temperature uniformly；
（6）By the tunica fibrosa of modification in step（5）2-4h is impregnated in solution obtained, then freeze-drying exists to being dehydrated
Hot pressing 20min in 35-45 DEG C of heating platen obtains the Medical absorbable hemostatic material.
2. a kind of preparation method of Medical absorbable hemostatic material according to claim 1, it is characterised in that：The step
（1）The volume ratio of middle dichloromethane and n,N-Dimethylformamide is 4:1, mixing time 5-8h, drying temperature 70-80
℃。
3. a kind of preparation method of Medical absorbable hemostatic material according to claim 1, it is characterised in that：The step
（2）The time of middle ultrasonic shear is 50min.
4. a kind of preparation method of Medical absorbable hemostatic material according to claim 1, it is characterised in that：The step
（3）Middle a concentration of 0.1wt% of EDS.
5. a kind of preparation method of Medical absorbable hemostatic material according to claim 1, it is characterised in that：The step
（4）A concentration of 0.05-0.15wt% of hyaluronic acid solution, holding time 15-25h, centrifugation time 5min, rotating speed are
1200-1600r/min。
6. thoroughly doing away with a kind of preparation method of Medical absorbable hemostatic material described in claim 1, it is characterised in that：The step
（5）In extract extracted by 50-75wt% ethyl alcohol.
7. a kind of preparation method of Medical absorbable hemostatic material according to claim 1, it is characterised in that：The step
（5）Raw material is weighed by following parts by weight, 1.2-2 parts of fragile blueberry root leaf extract, 1.5-2.5 parts of Orbicular Lindsaea extract, watt
0.8-0.4 parts of Wei extract, 0.6-1 parts of canarium pimela extract, 1.5-2.5 parts of glycine, -2.6 parts of arginase 12, chitosan sulfate
2-4 parts of ester, 1.8-2.2 parts of glutathione, 45-55 parts of medical distilled water.
8. a kind of preparation method of Medical absorbable hemostatic material according to claim 1, it is characterised in that：The step
（6）Middle soaking time is 3h, and hot pressing plate temperature is 40 DEG C.
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| CN113478601A (en) * | 2021-08-04 | 2021-10-08 | 广西容县美柏工艺品有限公司 | Dyeing method for improving dyeing fastness of woven rattan |
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