CN105012040A - Model-making method of hepatic stagnation and spleen deficiency type functional dyspepsia model for rats - Google Patents
Model-making method of hepatic stagnation and spleen deficiency type functional dyspepsia model for rats Download PDFInfo
- Publication number
- CN105012040A CN105012040A CN201510291105.1A CN201510291105A CN105012040A CN 105012040 A CN105012040 A CN 105012040A CN 201510291105 A CN201510291105 A CN 201510291105A CN 105012040 A CN105012040 A CN 105012040A
- Authority
- CN
- China
- Prior art keywords
- rat
- model
- group
- modeling
- electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a model-making method of hepatic stagnation and spleen deficiency type functional dyspepsia model for rats, pertaining to fields of medical evaluation and detection technology. The model-making method is characterized in that after healthy Wistar male rats are subjected to electrode-embedding operation, simulation of each rat by clipping tails, overfatigue and operation for drinking and eating disorder can be carried out in order to perform composite model-making operation; by means of weight, electrophysiology activities, gastric emptying, intestine propulsion and objective detection of molecular biology, the purpose of complying a made hepatic stagnation and spleen deficiency type FD model and diagnostic criteria for a clinical hepatic stagnation and spleen deficiency type FD theory is fulfilled in order to achieve consistency of clinical and basic science study; and acquired animals of the hepatic stagnation and spleen deficiency type functional dyspepsia model have symptoms of dyspepsia without other organic pathologic changes.
Description
Technical field
The invention belongs to medical care evaluation, detection technique field, relate to a kind of modeling method of animal model, be specifically related to a kind of modeling method of liver-depression and spleen-insufficiency type functional dyspepsia rat model.
Background technology
Functional dyspepsia (functional dyspepsia, FD) be typical somatopsychic illness, psychology-social factor plays important influence factor to morbidity, and feelings will, psychology, society and various biological factor interact, and jointly take part in the generation of FD.In recent years, the sickness rate of FD shows a rising trend, Epidemiological study shows, American-European countries's prevalence is 20% ~ 25%, Asian countries's sickness rate is about 8%-23%, the prevalence of China FD is totally higher, and investigation Shanghai has 782 patients of indigestion symptom for more than 18 years old, finds that FD patient accounts for 69%.The symptom of high incidence and recurrent exerbation or chronic delay, reduces quality of life, causes negative effect to body & mind psychology.The main Bian symptomatic treatment of the treatment of modern medicine to FD and Comprehensive Treatment; many employings promote digestive tract power reinforcing medicine; achieve certain clinical efficacy; but it all may increase the weight of symptom to patients with arrhythmia, Tumor Patients after Chemotherapy and hypokalemia patient; and medicine is through Liver and kidney metabolism; should not use for long-term hepatic and kidney function obstacle person, so it has certain limitation.The traditional Chinese medical science thinks that emotional stimulation is the key factor of functional dyspepsia morbidity, and syndrome of stagnation of liver qi and spleen deficiency is its main pathogenesis, and a large amount of report clinically illustrates that differentiation of tcm treatment functional dyspepsia has significant clinical efficacy.
Controlling according to the typing of etiology and pathogenesis opinion is the principle for the treatment of of the traditional Chinese medical science, usually the pattern of syndrome of FD can be divided into caused by hepatic stagnation qi stagnation, liver-depression and spleen-insufficiency type, spleen-stomach deficiency-cold, insufficiency of spleen-qi type, insufficiency of stomach drink stops type five class, the animal model making different pattern of syndrome according to etiology and pathogenesis is then even more important, wherein, syndrome of stagnation of liver qi and spleen deficiency is mainly based on emotional stimulation, the clinical common TCM Syndrome Type animal model preparation method of existing liver-depression and spleen-insufficiency type FD has: Chen Suning etc. adopt the folder anal spine of improvement Guo Haijun etc. to swash legal system for FD stagnation of liver-QI with deficiency of the spleen model, i.e. continuous tweezers clamp rat tail end 1/3 place, every daily Changhai, make its scream struggle but broken skin for degree, make its rage and beat up with other rats, each lasting 30 minutes, every day 2 times, continued stimulus 3 weeks, during modeling, each group of rat ad lib and drinking-water, rat appearance is restless waits anxiety, anxiety reaction, and food-intake obviously reduces, then think that modeling is successful, Zhang Guoping etc. make stagnation of liver-QI with deficiency of the spleen rat model with compound chronic constriction legal system, model group rats is bound by special constraint frame, the head and tail of rat bar is fixed on two of frame, make rat arbitrarily can not overturn health during fettering and movable, fetter 3 hours every day, morning 8:00 ~ 11:00, rat model is put in the large Plastic Drum went swimming filling warm water (22 ± 1) DEG C by 14:00, time is 10 minutes, feeding method next day that intemperance of taking food adopting (next day fasting, the next day give enough foods), continuous 3 weeks, the every cage of attack group rat is first put one by Han Qiuyan etc., with tip parcel gauze and large curved hemostat, clamps the tail attacking Mus, rat rage, itself and other rat is made to beat up, each stimulation 30 minutes, 3 times on the one, start after 3 days to use Radix Et Rhizoma Rhei gavage, every day 2 times, folder tail changes 2 times on the one into, each 15 minutes, gavage adds folder tail and carries out totally 10 days simultaneously, and modeling amounts to 13 days.
Above-mentioned existingly exist many defects for liver-depression and spleen-insufficiency type FD scale-model investigation: model production method is more single, and it is all use the folder tail of Guo Haijun etc. to enrage stimulus method that such as a large amount of bibliographical informations prepares stagnation of liver-QI with deficiency of the spleen model; Some model lacks the successful objective detection standard of modeling, the FD animal model of foundation theory of Chinese medical science and dyspeptic etiology theoretical modeling, should meet the diagnostic criteria of FD, FD animal pattern not only should have dyspeptic disease but also this animal must without other organic diseases; In addition, also lack the good and bad comparative study of different modeling method, simultaneously for concrete group match, and success rate and mortality rate all hand over unclear or not explanation.
Along with the development of TCM Modernization, about the animal experiment study of Traditional Chinese Medicine Function mechanism also gets more and more, the animal model preparation meeting TCM Syndrome Type becomes crucial.Therefore to strengthen leakage detection to fill a vacancy, research prepared by gradual perfection TCM Syndrome Type animal model in research in the future, better to carry out the pharmacological effect research of Chinese medicine.
Summary of the invention
The present invention is directed to the above-mentioned defect that prior art exists, a kind of modeling method of liver-depression and spleen-insufficiency type functional dyspepsia rat model is provided, the method enrages based on stimulus method by the folder tail of Guo Haijun etc., improve, mainly take to press from both sides that anal spine swashs, means compound that is overtired, drinking and eating irregularly carries out modeling, and by body weight, bioelectrical activity, gastric emptying, Intestinal pushing and molecular biological detection, reach the object that the theoretical diagnostic criteria of made liver-depression and spleen-insufficiency type FD model and liver-depression and spleen-insufficiency type FD is consistent, make clinically to reach concordance with basic research.
To achieve these goals, following technical scheme is adopted to be achieved:
A modeling method for liver-depression and spleen-insufficiency type functional dyspepsia rat model, comprises following concrete steps:
(1) conventional raising
Get the healthy Wistar male rat of body weight 180g-220g, raise 7 days in the receptacle routine of 45-65% at indoor temperature 20 DEG C-28 DEG C, relative humidity, period freely drinks water, feed;
(2) the embedding art of electrode
Conventional raising is in the embedding art of the descending electrode of aseptic condition after 7 days, and postoperative point of cage routine is raised, and recovers one week;
(3) gastrointestinal electrical activity is recorded
Water 18-24h is can't help in fasting on the same day in postoperative a week, and electrode cable is connected with the biological technical ability experimental system of BL-420S by next day, record normal rat Gastrointestinal Myoelectric Activity;
(4) modeling
1. use 1/3 place of oval clamp rat tail China and foreign countries, with the resistance of rat indignation or bait as degree, try not to clip broken the skin of afterbody, successively stimulate folder tail 30min at every turn;
2. press from both sides after anal spine swashs end and rat is placed on tired transfer rod instrument, make its running 10min, rotating speed 35r/min;
3. step operation 1. and is 2. repeated every 3h every day, every day carries out 4 times, continuous modeling 10 days, the feeding next day of simultaneously in these 10 days, observes the hair color of rat, hogback, addicted to asthenia, weak, irritability situation, and records body weight, body temperature, appetite, amount of drinking water, rat stomach Intestinal myoelectric activity, feces situation, to meeting the theoretical diagnostic criteria of FD, modeling terminates, and namely obtains liver-depression and spleen-insufficiency type FD rat model, records bioelectrical activity again after modeling terminates.
The embedding art of described electrode aseptically operates, preoperative self-control needle electrode, and configure 0.3% pentobarbital sodium anaesthetic, experimental day intraperitoneal injection of anesthesia rat 1ml/100g, abdominal part and nape portion are shaved Mao Bingyong 0.5% povidone iodine routine disinfection, paving sterilization hole towel, xiphoid-process Ventral Midline line otch, take out Stomach duodenum, by 2, muscle layer under gastric antrum and duodenal serosa is embedded in respectively to self-control needle electrode.Pair of electrodes is embedded in gastric antrum place, about 1cm on pylorus, second pair of electrode is placed in duodenum apart from pylorus 1-2cm place, bipolar electrode is along gastric antrum and intestinal tube longitudinal arrangement, spacing 5mm, electrode cable is walked to walk to nape portion pass fixing along subcutaneous, several penicillin injection liquid are dripped to prevent abdominal cavity infection in abdominal cavity, layer-by-layer suture wound, postoperative with aseptic 0.9%NaCl injection configuration penicillin injection liquid 0.8 ten thousand U/ml, every rat 1ml lumbar injection, every day 1 time, continuous injection 3 days, to prevent abdominal cavity infection.
In described step (4), folder anal spine swashs must, at 1/3 place of rat tail China and foreign countries, be avoided pressing from both sides tail base as far as possible, folder tail base may clip to caudal vertebra,, there is the possibility of fecaluria incontinence, thus correct model cannot be set up in the local paralysis that strong impulse may make rat occur.
In described step (4)-2., after folder anal spine swashs and terminates, experimenter first trains rat, then makes it run voluntarily on tired transfer rod instrument.
Compared with prior art, the invention has the beneficial effects as follows: (1) take to swash with a single only folder anal spine, means compound that is overtired and drinking and eating irregularly carries out modeling, and by body weight, bioelectrical activity, gastric emptying, Intestinal pushing and molecular biological objective detection, reach the object that the theoretical diagnostic criteria of made liver-depression and spleen-insufficiency type FD model and liver-depression and spleen-insufficiency type FD is consistent, make clinically to reach concordance with basic research; (2) the modeling method that compensate for Guo Haijun etc. can only form the defect of simple stagnation of liver-QI model, the modeling method of liver-depression and spleen-insufficiency type FD model is made to improve further and improve, the liver-depression and spleen-insufficiency type FD animal pattern obtained not only has dyspeptic disease, and without other organic diseases.
Accompanying drawing explanation
Experimental rat normal group gastric slow wave and intestinal slow wave testing result in Fig. 1 embodiment of the present invention;
Experimental rat normal group stomach fast wave and intestinal fast wave testing result in Fig. 2 embodiment of the present invention;
Experimental rat model group gastric slow wave and intestinal slow wave testing result in Fig. 3 embodiment of the present invention;
Experimental rat model group stomach fast wave and intestinal fast wave testing result in Fig. 4 embodiment of the present invention;
The ELISA testing result of experimental rat serum gastrointestinal mucin (Ghrelin) in Fig. 5 embodiment of the present invention;
The ELISA testing result of experimental rat leptin (Leptin) in Fig. 6 embodiment of the present invention;
The ELISA testing result of experimental rat 5-hydroxy tryptamine (5-hydroxytryptamine, 5-HT) in Fig. 7 embodiment of the present invention;
The ELISA testing result of experimental rat vasoactive intestinal peptide (Vasoactive intestinalpeptide, VIP) in Fig. 8 embodiment of the present invention.
Detailed description of the invention
Embodiment
In order to prove the effectiveness of the modeling method of this kind of liver-depression and spleen-insufficiency type functional dyspepsia rat model, carry out following experiment:
1 material
1.1 laboratory animal
Healthy Wistar male rat 80, body weight (200 ± 20) g, is provided by Gansu Chinese of Traditional Chinese Medicine's animal experimental center, credit number: SCKX (sweet) 2014-0001.
1.2 medicine
Pingwei Capsule is on the basis of Pingwei San in the Song dynasty " formulary of peaceful benevolent dispensary ", comprehensive modern medicine pathogeny, modern pharmacological research and clinical experience addition or subtraction of changes for many years.Be made up of 16 taste Chinese medicines such as parched with bran Rhizoma Atractylodis, Cortex Magnoliae Officinalis, stir-baked Fructus Aurantii in bran, Pericarpium Citri Reticulataes.Be approved as preparation in institute of Affiliated Hospital of Gansu University of Traditi in 2012, authentication code is: sweet medicine word Z120022224, and effect is soothing liver and strengthening spleen, heat clearing and damp drying, Analgesia; Motilium (domperidone tablet), authentication code: the accurate word H10910003 of traditional Chinese medicines, is produced by Xian-Janssen Pharmaceutical Ltd..
The preparation that 1.3 trophism semisolids are stuck with paste
The preparation that trophism semisolid is stuck with paste: get Sodium Tvlose 5g, be dissolved in 100ml distilled water, add milk powder 8g, sugared 4g, starch 4g, burnt black ink 2ml respectively, stir, prepare the black semisolid pastel into about 150ml, refrigerator cold-storage, the used time returns to room temperature.
1.4 reagent
Serum gastrointestinal mucin (Ghrelin), leptin (Leptin), 5-hydroxy tryptamine (5-hydroxytryptamine, 5-HT) with vasoactive intestinal peptide (Vasoactive intestinalpeptide, VIP) Elisa test kit, by Shanghai, Heng Yuan bio tech ltd provides.
1.5 instrument
MA-100A type microplate reader (Hangzhou Bo Te instrument and equipment company limited), GNP-9080 type water isolation type constant incubator (the upper grand experimental facilities company limited of Nereid), pipettor, the biological technical ability experimental system of BL-420S, TGL-16 high speed desktop refrigerated centrifuger (Changsha Xiang Yi centrifugal apparatus company limited), tired transfer rod instrument (Chengdu TME Technology Co., Ltd.) of ZB-200.
2 methods
2.1 animal model methods
Get ready experimental rat, raise 7 days at indoor temperature 20 DEG C-28 DEG C, relative humidity at the receptacle of 45-65%, in the embedding art of the descending electrode of aseptic condition, preoperative self-control needle electrode, configures 0.3% pentobarbital sodium anaesthetic.Intraperitoneal injection of anesthesia rat 1ml/100g, abdominal part and nape portion are shaved Mao Bingyong 0.5% povidone iodine routine disinfection, paving sterilization hole towel, xiphoid-process Ventral Midline line otch, take out stomach, duodenum, by 2, muscle layer under gastric antrum and duodenal serosa is embedded in respectively to self-control needle electrode, pair of electrodes is embedded in gastric antrum place, about 1cm on pylorus, second pair of electrode is placed in duodenum apart from pylorus 1-2cm place, bipolar electrode is along gastric antrum and intestinal tube longitudinal arrangement, spacing 5mm, electrode cable is walked to walk to nape portion pass fixing along subcutaneous, several penicillin injection liquid are dripped to prevent abdominal cavity infection in abdominal cavity, layer-by-layer suture wound, postoperative with aseptic 0.9%NaCl injection configuration penicillin injection liquid 0.8 ten thousand U/ml, every rat 1ml lumbar injection, every day 1 time, continuous injection 3 days, to prevent abdominal cavity infection, postoperative point of cage routine is raised, after recovering one week, water 18-24h is can't help in fasting, wire is connected with the biological technical ability experimental system of BL-420S, first record rat stomach Intestinal myoelectric activity, as normal control.Carry out modeling afterwards: with 1/3 place of oval clamp rat tail China and foreign countries, with the resistance of rat indignation or bait as degree, try not to clip broken the skin of afterbody, successively stimulate folder tail 30min at every turn; After folder anal spine swashs end, rat is placed on tired transfer rod instrument, makes its running 10min, rotating speed 35r/min; Repeat folder anal spine every 3h every day and swash the operation with tired transfer rod instrument road-work, every day carries out 4 times, continuous modeling 10 days, the feeding next day of in 10 days, observe the hair color of rat, hogback, addicted to asthenia, flock together, weak, irritability situation, and record body weight, body temperature, appetite, amount of drinking water, rat stomach Intestinal myoelectric activity, feces situation, to meeting the theoretical diagnostic criteria of FD, modeling terminates.
2.2 animal groupings
Random packet is carried out: normal control 8, all the other rats carry out modeling after first record rat normal gastric Intestinal myoelectric activity.Rat model is divided into 5 groups at random, and be respectively the high, medium and low dosage group of Pingwei Capsule, motilium group, model group, often organize each 8, Normal group 8, normal group does not give any stimulation, ad lib water, freely movable.
2.3 medication
After modeling completes, be converted to dose,equivalent by people and rat body surface area ratio.The high, medium and low dosage component of Pingwei Capsule not An 24 times, 16 times, 8 multiple doses convert after, Pingwei Capsule is not configured to 0.24g/ml, 0.16g/ml, 0.08g/ml all with 1ml/100g gavage by high, medium and low dosage component; Motilium group with the suspension of 0.5mg/ml with 1ml/100g gavage, model group and Normal group all with normal saline gavage, six groups of equal every days 2 times, administration 3 weeks.
2.5 collections of specimens and detection
(1) gastrointestinal electrical activity is recorded
Administration, after three weeks, records gastrointestinal electrical activity situation again.
(2) get blood and carry out the test of gastric emptying Intestinal pushing
1. after water 18-24h is can't help in fasting, with 5% carbon powder suspension 1ml/100g gastric infusion, after 20min, fallen by rat dizzy, femoral artery is taken a blood sample, after room temperature leaves standstill 1-2 hour, centrifugally under 4 DEG C of conditions get the supernatant and serum with the centrifugal 10Min of 3500-4000rpm rotating speed, be sub-packed in centrifuge tube, leave in-80 DEG C of refrigerators, intend day row Elisa and detect.
2. open abdominal cavity, ligation stomach cardia and pylorus, take out stomach, weigh after blotting with filter paper, then cut off body of stomach along greater gastric curvature, wash away gastric content, then blot with filter paper, claim stomach net weight.Calculate gastric emptying (%)=1-[(stomach full weight-stomach net weight)/semi-solid paste] × 100%, evaluate gastric emptying speed with this.
3. then take out small intestinal naturally to straighten and be laid on blank sheet of paper, measure total small intestinal length L (pylorus is to the distance of ileocecus), charcoal end advance distance L
0(pylorus advances front end to charcoal end).Intestinal propulsion degree (%)=L
0/ L × 100%, evaluates intestinal propulsion speed with this.
2.6 statistical procedures
Adopt SPSSl9.0 software to analyze, experimental data is with mean ± standard deviation
represent, between group, compare the variance analysis adopting single-factor variable.Statistical significance is indicated with p<0.05.
3 results
3.1 physiologys observe
Observe and record ordinary circumstances such as model experiment rat hair color, feces, mobility and the emotions before and after modeling, result is as follows:
Table 1 physiology observed result
| Project | Before modeling | After modeling | After treatment |
| Hair color | Smooth | Withered and yellowly to have some setbacks | Take a turn for the better |
| Feces | Normally | Rare half congealed | Take a turn for the better |
| Mobility | Active | Weak, addicted to asthenia | Take a turn for the better |
| Emotion | Normally | Anxiety, anxiety, anger | Take a turn for the better |
3.2 food-intakes detect
Measure respectively the food-intake before and after modeling and after Drug therapy, body weight, testing result is as follows:
Δ
The change of table 2 rats eating amount (n=8,
)
| Group | Dosage (g/ml) | Before modeling (g) | After modeling (g) | After treatment (g) |
| Normal group | -- | 30.7±1.4 | 31.7±2.0 | 33.9±2.1 |
| Model group | -- | 30.0±2.1 | 23.4±0.9﹡ | 24.5±1.0 |
| Motilium group | 0.0005 | 29.8±2.5 | 23.2±1.3﹡ | 29.6±1.1 Δ |
| High dose group | 0.24 | 30.5±1.8 | 24.0±1.5﹡ | 29.9±1.3 Δ★ |
| Middle dosage group | 0.16 | 29.8±1.7 | 23.6±0.8﹡ | 28.9±1.0 Δ★ |
| Low dose group | 0.08 | 29.8±2.1 | 23.6±1.6﹡ | 26.9±1.1 Δ |
Note: compare with normal group
﹡p<0.01, compares with model group
Δp<0.01, compares with motilium group
★p>0.05.
The change of table 3 rat body weight (n=8,
)
Note: compare with normal group
﹡p<0.01, compares with model group
Δp<0.05, compares with motilium group
★p>0.05.
3.3 gastrointestinal electrical activities detect
Measure respectively the Stomach duodenum electricity slow wave amplitude before and after modeling and after Drug therapy, frequency, fast wave-amplitude, incidence rate, testing result is as follows:
The change of table 4 gastric electrical activity (n=8,
)
Note: compare with normal group
﹡p<0.01, compares with model group
Δp<0.01, compares with motilium group
★p>0.05.
The electrophysiological change of table 5 duodenum (n=8,
)
Note: compare with normal group
﹡p<0.01, compares with model group
Δp<0.01, compares with motilium group
★p>0.05.
3.4 gastric emptying rates and intestinal propulsion detect
Measure respectively the gastric emptying rate before and after modeling and after Drug therapy, intestinal propulsion percentage rate, testing result is as follows:
The change of table 6 gastric emptying and intestinal propulsion degree (n=8,
)
| Group | Dosage (g/ml) | Gastric emptying (%) | Intestinal pushing (%) |
| Normal group | -- | 78.1±1.9 | 70.0±1.7 |
| Model group | -- | 62.6±2.3 ﹡ | 53.1±1.9 ﹡ |
| Motilium group | 0.0005 | 73.5±1.6 Δ | 69.3±2.1 Δ |
| High dose group | 0.24 | 73.2±2.1 Δ★ | 69.6±1.7 Δ★ |
| Middle dosage group | 0.16 | 71.5±2.1 Δ | 65.2±2.9 Δ |
| Low dose group | 0.08 | 68.9±2.0 Δ | 61.0±2.9 Δ |
Note: compare with normal group
﹡p<0.01, compares with model group
Δp<0.01, compares with motilium group
★p>0.05.
3.5 serum gastrointestinal mucin and leptin content detect
Measure respectively the serum gastrointestinal mucin before and after modeling and after Drug therapy, leptin content, testing result is as follows:
The change of table 7 serum gastrointestinal mucin (n=8,
)
| Group | Dosage (g/ml) | Gastrointestinal mucin content (ng/L) |
| Normal group | -- | 1486.23±114.85 |
| Model group | -- | 749.13±55.63 ﹡﹡ |
| Motilium group | 0.0005 | 1360.53±168.58 ΔΔ |
| High dose group | 0.24 | 1517.67±95.33 ΔΔ★ |
| Middle dosage group | 0.16 | 1011.72±89.25 ΔΔ |
| Low dose group | 0.08 | 933.59±82.23 Δ |
Note: compare with normal group
﹡ ﹡p<0.01; Compare with model group
Δp<0.05;
Δ Δp<0.01, compares with positive controls
★p>0.05.
The change of table 8 Serum Leptin Levels content (n=8,
)
| Group | Dosage (g/ml) | Leptin (μ g/L) |
| Normal group | -- | 2.32±0.11 |
| Model group | -- | 3.24±0.42 ﹡﹡ |
| Motilium group | 0.0005 | 2.28±0.10 ΔΔ |
| High dose group | 0.24 | 2.46±0.09 ΔΔ★ |
| Middle dosage group | 0.16 | 2.82±0.12 Δ |
| Low dose group | 0.08 | 2.72±0.09 Δ |
Note: compare with normal group
﹡ ﹡p<0.01; Compare with model group
Δp<0.05;
Δ Δp<0.01; With motilium group ratio
★p>0.05.
3.6 serum 5-HTs and vasoactive intestinal peptide content detection
Measure respectively the serum 5-HT before and after modeling and after Drug therapy, vasoactive peptide content, testing result is as follows:
The change of table 9 serum 5-HT (n=8,
)
| Group | Dosage (g/ml) | 5-hydroxy tryptamine (ng/L) |
| Normal group | -- | 314.64±10.11 |
| Model group | -- | 228.15±7.82 ﹡﹡ |
| Motilium group | 0.0005 | 282.35±6.05 ΔΔ |
| High dose group | 0.24 | 286.72±7.74 ΔΔ★ |
| Middle dosage group | 0.16 | 263.14±8.75 ΔΔ |
| Low dose group | 0.08 | 257.01±6.27 Δ |
Note: compare with normal group
﹡ ﹡p<0.01; Compare with model group
Δp<0.05;
Δ Δp<0.01; With motilium group ratio
★p>0.05.
The change of table 10 vasoactive peptide content (n=8,
)
| Group | Dosage (g/ml) | Vasoactive intestinal peptide (ng/L) |
| Normal group | -- | 79.95±2.33 |
| Model group | -- | 136.65±4.02 ﹡﹡ |
| Motilium group | 0.0005 | 102.49±4.86 ΔΔ |
| High dose group | 0.24 | 97.93±4.15 ΔΔ★ |
| Middle dosage group | 0.16 | 112.36±4.48 ΔΔ |
| Low dose group | 0.08 | 124.64±4.74 Δ |
Note: compare with normal group
﹡ ﹡p<0.01; Compare with model group
Δp<0.05;
Δ Δp<0.01; With motilium group ratio
★p>0.05.
4 experiment conclusion
(1), before experiment starts, each group rat body weight there are no significant difference, hair is smooth, and feces is normal, enlivens active.After modeling, rat model hair is withered and yellow to have some setbacks, loose stool, typical insufficiency of the spleen symptoms such as mobility minimizing, and have anxiety, anxiety, time and and send " z z z " anger etc. stagnation of liver-QI symptom, dietary amount, body weight more all reduce with normal group, and use has the motilium of good efficacy and Pingwei Capsule to treat rat model to liver-depression and spleen-insufficiency type FD, find that above-mentioned situation all take a favorable turn, diet, body weight all increase.
(2) each group rats eating amount, body weight zero difference (p>0.05) before modeling; After modeling, compared with normal group, model group experimental rat food-intake, body weight obviously reduce, and have significant difference (P < 0.01); After treatment compared with model group, Pingwei Capsule group and motilium group dietary amount, body weight obviously increase, and all have significant difference (P < 0.01).
(3) model group Stomach duodenum electricity slow wave amplitude, frequency, fast wave-amplitude, incidence rate are all lower than normal group, and significant difference (p<0.01), after using the treatment of Pingwei Capsule, motilium group, stomach, intestinal electricity slow wave amplitude, frequency, fast wave-amplitude, incidence rate all higher than model group, significant difference (p<0.01).
(4) compared with normal group, gastric emptying, the Intestinal propulsive rate of model group rats obviously reduce, and all have significant difference (P < 0.01); Compared with model group, Pingwei Capsule group and motilium group gastric emptying, Intestinal propulsive rate obviously raise, and all have significant difference (P < 0.05).
(5) compared with normal group, model group serum Ghrelin content obviously reduces, and leptin content obviously raises, and has significant difference (P < 0.01); Compared with model group, Pingwei Capsule group and motilium group serum Ghrelin content obviously raise, and leptin content obviously reduces, and all has significant difference (P < 0.05, p<0.01).
(6) compared with normal group, model group serum 5-HT content obviously reduces, and VIP content obviously raises, and has significant difference (P < 0.01); Compared with model group, Pingwei Capsule group and motilium group serum 5-HT content obviously raise, and VIP content obviously reduces, and all has significant difference (P < 0.05, p<0.01).
In sum, compared with normal rat, measure on the feed with the rat model that the modeling method of liver-depression and spleen-insufficiency type functional dyspepsia rat model of the present invention makes, body weight, harmonization of the stomach duodenum electric wave, gastric emptying, Intestinal propulsive rate, serum Ghrelin content, leptin content, 5-HT content, VIP content aspect all have significant difference, and after using and having the Pingwei Capsule of good definite curative effect and motilium to treat to liver-depression and spleen-insufficiency type functional dyspepsia, the revolution of rat model indices is normal.As can be seen here, the modeling method of liver-depression and spleen-insufficiency type functional dyspepsia rat model of the present invention is successfully effective, is suitable for actual clinical Study and appliance and promotes.
Claims (3)
1. a modeling method for liver-depression and spleen-insufficiency type functional dyspepsia rat model, is characterized in that: comprise following concrete steps:
(1) conventional raising
Get the healthy Wistar male rat of body weight 180g-220g, raise 7 days in the receptacle routine of 45-65% at indoor temperature 20 DEG C-28 DEG C, relative humidity, period freely drinks water, feed;
(2) the embedding art of electrode
Conventional raising is in the embedding art of the descending electrode of aseptic condition after 7 days, and postoperative point of cage routine is raised, and recovers one week;
(3) record gastrointestinal electrical activity
Water 18-24h is can't help in fasting on the same day in postoperative a week, and electrode cable is connected with the biological technical ability experimental system of BL-420S by next day, record normal rat Gastrointestinal Myoelectric Activity;
(4) modeling
1. use 1/3 place of oval clamp rat tail China and foreign countries, with the resistance of rat indignation or bait as degree, try not to clip broken the skin of afterbody, avoid pressing from both sides tail base, successively stimulate folder tail 30min at every turn;
2. press from both sides after anal spine swashs end and rat is placed on tired transfer rod instrument, make its running 10min, rotating speed 35r/min;
3. step operation 1. and is 2. repeated every 3h every day, every day carries out 4 times, continuous modeling 10 days, the feeding next day of simultaneously in these 10 days, observes the hair color of rat, hogback, addicted to asthenia, weak, irritability situation, and records body weight, body temperature, appetite, amount of drinking water, rat stomach Intestinal myoelectric activity, feces situation, to meeting the theoretical diagnostic criteria of FD, modeling terminates, and namely obtains liver-depression and spleen-insufficiency type FD rat model, records bioelectrical activity again after modeling terminates.
2. the modeling method of a kind of liver-depression and spleen-insufficiency type functional dyspepsia rat model according to claim 1, it is characterized in that: the embedding art of described electrode aseptically operates, preoperative self-control needle electrode, configure 0.3% pentobarbital sodium, intraperitoneal injection of anesthesia rat (1ml/100g), abdominal part and nape portion are shaved Mao Bingyong 0.5% povidone iodine routine disinfection, paving sterilization hole towel, xiphoid-process Ventral Midline line otch, take out stomach, duodenum, by 2, muscle layer under gastric antrum and duodenal serosa is embedded in respectively to self-control needle electrode, pair of electrodes is embedded in gastric antrum place, about 1cm place on pylorus, second pair of electrode is placed in duodenum apart from pylorus 1-2cm place, bipolar electrode is along gastric antrum and intestinal tube longitudinal arrangement, spacing 5mm, electrode cable is walked to walk to nape portion pass fixing along subcutaneous, several penicillin injection liquid are dripped to prevent abdominal cavity infection in abdominal cavity, layer-by-layer suture wound, postoperative with aseptic 0.9%NaCl injection configuration penicillin injection liquid 0.8 ten thousand U/ml, every rat 1ml lumbar injection, every day 1 time, continuous injection 3 days, to prevent abdominal cavity infection.
3. the modeling method of a kind of liver-depression and spleen-insufficiency type functional dyspepsia rat model according to claim 1, it is characterized in that: in described step (4)-2., after folder anal spine swashs end, experimenter first trains rat, then makes it run voluntarily on tired transfer rod instrument.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510291105.1A CN105012040A (en) | 2015-05-29 | 2015-05-29 | Model-making method of hepatic stagnation and spleen deficiency type functional dyspepsia model for rats |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510291105.1A CN105012040A (en) | 2015-05-29 | 2015-05-29 | Model-making method of hepatic stagnation and spleen deficiency type functional dyspepsia model for rats |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105012040A true CN105012040A (en) | 2015-11-04 |
Family
ID=54402603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510291105.1A Pending CN105012040A (en) | 2015-05-29 | 2015-05-29 | Model-making method of hepatic stagnation and spleen deficiency type functional dyspepsia model for rats |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105012040A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070052A (en) * | 2016-07-07 | 2016-11-09 | 王涛 | A kind of method building the irritability stasis of blood stagnant card animal model |
| CN106165671A (en) * | 2016-07-31 | 2016-11-30 | 甘肃农业大学 | A kind of method setting up diarrhea due to damp-heat animal model |
| CN108815706A (en) * | 2018-05-23 | 2018-11-16 | 广州中医药大学(广州中医药研究院) | The method for establishing the mouse model of functional dyspepsia FD |
| CN110558285A (en) * | 2019-11-05 | 2019-12-13 | 江西中医药大学 | Animal model construction method for spleen deficiency and excessive dampness syndrome research and application thereof |
| CN112106729A (en) * | 2020-10-28 | 2020-12-22 | 童萍 | Modeling method for mice with depression, liver depression and spleen deficiency syndrome |
| CN113491251A (en) * | 2021-07-02 | 2021-10-12 | 山西中医药大学 | Kidney-yang deficiency type Parkinson disease rat model and preparation method thereof |
-
2015
- 2015-05-29 CN CN201510291105.1A patent/CN105012040A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070052A (en) * | 2016-07-07 | 2016-11-09 | 王涛 | A kind of method building the irritability stasis of blood stagnant card animal model |
| CN106165671A (en) * | 2016-07-31 | 2016-11-30 | 甘肃农业大学 | A kind of method setting up diarrhea due to damp-heat animal model |
| CN108815706A (en) * | 2018-05-23 | 2018-11-16 | 广州中医药大学(广州中医药研究院) | The method for establishing the mouse model of functional dyspepsia FD |
| CN108815706B (en) * | 2018-05-23 | 2022-02-01 | 广州中医药大学(广州中医药研究院) | Method for establishing mouse model of functional dyspepsia |
| CN110558285A (en) * | 2019-11-05 | 2019-12-13 | 江西中医药大学 | Animal model construction method for spleen deficiency and excessive dampness syndrome research and application thereof |
| CN112106729A (en) * | 2020-10-28 | 2020-12-22 | 童萍 | Modeling method for mice with depression, liver depression and spleen deficiency syndrome |
| CN113491251A (en) * | 2021-07-02 | 2021-10-12 | 山西中医药大学 | Kidney-yang deficiency type Parkinson disease rat model and preparation method thereof |
| CN113491251B (en) * | 2021-07-02 | 2022-12-20 | 山西中医药大学 | Preparation method of kidney-yang deficiency type Parkinson disease rat model |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105012040A (en) | Model-making method of hepatic stagnation and spleen deficiency type functional dyspepsia model for rats | |
| CN103549074B (en) | Power lifting tea | |
| CN102641395B (en) | Medicament composite for treating constipation as well as preparation method and application of medicament composite | |
| CN102357198B (en) | Chinese medicinal composition for treating constipation | |
| CN103285092B (en) | Patch for treating infantile diarrhea caused by damp and heat | |
| CN102475205A (en) | Medicinal diet for treating constipation | |
| CN105106228B (en) | The pharmaceutical composition containing ginsenoside Rb1 as TMEM16A ion channel activators | |
| CN105796654A (en) | Traditional Chinese medicine composition for treating diarrhea irritable bowel syndrome, navel paste and application | |
| CN104623499B (en) | A kind of Chinese medicine preparation for aiding in acupuncture treatment DGP and preparation method and application | |
| CN104383423B (en) | A kind of Chinese medicine for treating sitophobia disease | |
| CN105770920B (en) | A kind of pharmaceutical preparation and its application for stomach and intestine color ultrasound assistant | |
| CN104784627A (en) | Traditional Chinese medicine externally used film agent used for treatment surgical open or closed injury and preparation method thereof | |
| CN109674980A (en) | A kind of Chinese medicine composition and preparation method thereof for treating constipation-predominant of irritable bowel syndrome | |
| CN108619432A (en) | A kind of preparation method for treating fat nano plaster | |
| CN104491041B (en) | Gegen Qinlian oral fluid and preparation method thereof | |
| CN102614426A (en) | Medicinal preparation and preparation method for helping digestion and removing stagnation | |
| CN104740302B (en) | A kind of Chinese medicine composition for treating Qi deficiency blood stasis type lumbar spinal stenosis | |
| CN103393965B (en) | Traditional Chinese medicine for treating wind dryness lung impairment type cough | |
| CN103191243A (en) | Application of medicament composition composed of coptis chinensis and fructus evodiae and preparation method of medicament composition | |
| CN107583023A (en) | Treat Chinese medicine preparation and the preparation of intestinal obstruction | |
| CN114344388B (en) | Traditional Chinese medicine composition for treating insomnia and application thereof | |
| CN103340402B (en) | Cream of peanuts, red dates and edible fungus, and preparation method and application of cream | |
| CN105477102A (en) | Traditional Chinese medicine for treating type 2 diabetes | |
| CN104547113B (en) | Chinese medicinal composition for treating post-menopausal female urethral syndrome | |
| CN102526369B (en) | The new application of Compound Zhongjiefeng |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151104 |