CN105010306A - Fox semen diluting liquid - Google Patents
Fox semen diluting liquid Download PDFInfo
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- CN105010306A CN105010306A CN201510388030.9A CN201510388030A CN105010306A CN 105010306 A CN105010306 A CN 105010306A CN 201510388030 A CN201510388030 A CN 201510388030A CN 105010306 A CN105010306 A CN 105010306A
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Abstract
The invention discloses a fox semen diluting agent. The raw materials for the semen diluting agent are all analytically pure. The diluting agent is composed of trihydroxymethyl aminomethane, citric acid, D-fructose, streptomycin, egg yolk and Ham's F-10. According to the invention, 2.13g of trihydroxymethyl aminomethane, 1.24g of citric acid and 0.71g of D-fructose are accurately weighed and dissolved in 30ml of double-distilled water; and the mixture is well mixed in 50ml of double-distilled water. The solution is filtered with double-layer qualitative filter paper, and is subjected to high-pressure sterilization; the solution is completely cooled, and 10ml of egg yolk, 10ml of Ham's F-10 and 70,000 U penicillin are added into the solution; and the mixture is stored under a temperature of 4 DEG C. When compared to a diluting agent purchased from the market, an artificial insemination litter size with the diluting agent provided by the invention is 6.5+/-1.5 (n=15), while an artificial insemination litter size with the diluting agent purchased from the market is 2.5+/-1.5 (n=185). Through a t test, the difference is significant. As a result, when semen is diluted with the diluting agent provided by the invention, more offspring can be obtained.
Description
Technical field
The present invention relates to liquefacient duration technical field, be specifically related to a kind of novel fox semen diluent.
Background technology
Fox belongs to vertebrate the carnivore of door, Mammalia, Carnivora, Canidae.The fox kind of the fox of artificial feeding mainly Vulpes and arctic Vulpes.The Representative Cultivars of Vulpes is silver-colored black fox, arctic Vulpes be blue fox.
Foster fox is the industry that economic benefit is higher, the current especially good social connections in rural prosperity especially.The whole body of fox is precious.Its skin is the high-grade goods of animal product, earns foreign exchange through export higher, but present this precious fur-bearing animal is raised and management exists a lot of difficult problems, particularly on the reproduction rate of its young baby.So people need a kind of new method of maturation at present, improve offspring's output capacity, fox cultivation obtains larger economic benefit.
Summary of the invention
It is simple that technical problem to be solved by this invention is to provide a kind of composition, can improve the novel fox semen diluent of fox offspring output capacity.
Technical problem to be solved by this invention realizes by the following technical solutions:
A kind of fox semen diluent, the raw materials used middle trishydroxymethylaminomethane of semen diluent, citric acid and D-Fructose are pure for analyzing, Ham's F-10 is for originating from Life technologies Products, and this dilution is formulated by the component of following weight and volume:
Compound method is as follows:
1) precise 2.13 grams of trishydroxymethylaminomethanes, 1.24 grams of citric acids and 0.71 gram of D-Fructose add 30 milliliters of distilled waters dissolvings, then add 50 milliliters of distilled waters mixings;
2) get Fresh Egg cleaned after put into 75% ethanol soak 30min take out, treat that vaporized alcohol is complete, in ultra-clean operating desk, egg shell broken, puncture vitellinae membrana with rifle head and carefully draw yolk to beaker, for subsequent use;
3) with double-deck qualitative filter paper by step 1) in solution filter after autoclave sterilization, completely cooling after add 10 milliliters successively by step 2) the yolk liquid prepared, 10 milliliters of Ham's F-10 and 70,000 U penicillin, regulate PH to 7.0;
4) store under 4 DEG C of environment.
Using method: take out dilution, in 28 DEG C of fertilizations indoor by its equalized temperature to room temperature, by 1:4 dilution proportion seminal fluid.
The invention has the beneficial effects as follows: semen diluent used in the present invention, test through Linquan County Fox farms, offspring is compared with the dilution bought from market in its artificial insemination farrowing, after this dilution artificial insemination farrowing, algebraically is 6.5 ± 1.5 (n=15 only), and the dilution artificial insemination farrowing offspring bought is 2.5 ± 1.5 (n=185 only), through t inspection, significant difference.Result shows that the semen diluent with this standard specifies dilutes seminal fluid, can the more offspring of output.
Embodiment
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.
Embodiment 1
A kind of fox semen diluent, semen diluent is raw materials used to be and to analyze pure, is made up of trishydroxymethylaminomethane, citric acid, D fructose, streptomycin, yolk liquid and Ham's F-10; Concrete weight is:
Compound method is as follows:
1) precise 2.13 grams of trishydroxymethylaminomethanes, 1.24 grams of citric acids and 0.71 gram of D-Fructose add 30 milliliters of distilled waters dissolvings, then add 50 milliliters of distilled waters mixings;
2) get Fresh Egg cleaned after put into 75% ethanol soak 30min take out, treat that vaporized alcohol is complete, in ultra-clean operating desk, egg shell broken, puncture vitellinae membrana with rifle head and carefully draw yolk to beaker, for subsequent use;
3) with double-deck qualitative filter paper by step 1) in solution filter after autoclave sterilization, completely cooling after add 10 milliliters successively by step 2) the yolk liquid prepared, 10 milliliters of Ham's F-10 and 70,000 U penicillin, regulate PH to 7.0;
4) store under 4 DEG C of environment.
5) using method: take out dilution, in 28 DEG C of fertilizations indoor by its equalized temperature to room temperature, by 1:4 dilution proportion seminal fluid.
Semen diluent used in the present invention, another Fox farms test through Linquan County, offspring is compared with the dilution bought from market in its artificial insemination farrowing, after this dilution artificial insemination farrowing, algebraically is 6.7 ± 1.6 (n=55 only), and the dilution artificial insemination farrowing offspring bought is 2.4 ± 1.3 (n=165 only), through t inspection, significant difference.Result shows to dilute seminal fluid with this semen diluent, can the more offspring of output after artificial insemination.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (2)
1. a fox semen diluent, is characterized in that, formulated by following component:
2. prepare a method for dilution described in claim 1, it is characterized in that, step is as follows:
1) precise 2.13 grams of trishydroxymethylaminomethanes, 1.24 grams of citric acids and 0.71 gram of D-Fructose add 30 milliliters of distilled waters dissolvings, then add 50 milliliters of distilled waters mixings;
2) get Fresh Egg cleaned after put into 75% ethanol soak 30min take out, treat that vaporized alcohol is complete, in ultra-clean operating desk, egg shell broken, puncture vitellinae membrana with rifle head and carefully draw yolk to beaker, for subsequent use;
3) with double-deck qualitative filter paper by step 1) in solution filter after autoclave sterilization, completely cooling after add 10 milliliters successively by step 2) the yolk liquid prepared, 10 milliliters of Ham's F-10 and 70,000 U penicillin, regulate PH to 7.0;
4) store under 4 DEG C of environment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510388030.9A CN105010306A (en) | 2015-06-30 | 2015-06-30 | Fox semen diluting liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510388030.9A CN105010306A (en) | 2015-06-30 | 2015-06-30 | Fox semen diluting liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105010306A true CN105010306A (en) | 2015-11-04 |
Family
ID=54400964
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510388030.9A Pending CN105010306A (en) | 2015-06-30 | 2015-06-30 | Fox semen diluting liquid |
Country Status (1)
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| CN (1) | CN105010306A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110622958A (en) * | 2016-06-29 | 2019-12-31 | 许红喜 | Animal sperm diluent |
| CN114403131A (en) * | 2022-01-21 | 2022-04-29 | 河北科技师范学院 | Semen cryopreservation liquid and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563359A (en) * | 2004-04-22 | 2005-01-12 | 上海交通大学 | Diluent for sperm of blue fox |
| CN1800369A (en) * | 2005-12-15 | 2006-07-12 | 上海交通大学 | Canine semen dilution liquid |
| CN102812950A (en) * | 2012-09-07 | 2012-12-12 | 江苏省家禽科学研究所 | Chicken semen diluent as well as preparation method and application thereof |
| CN102823580A (en) * | 2012-08-19 | 2012-12-19 | 四川省畜牧科学研究所 | Diluted preservation liquid for rabbit semen |
| CN104012520A (en) * | 2014-05-23 | 2014-09-03 | 山西省生态畜牧产业管理站 | Anti-oxidative cattle frozen semen diluent and preparation method thereof |
-
2015
- 2015-06-30 CN CN201510388030.9A patent/CN105010306A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1563359A (en) * | 2004-04-22 | 2005-01-12 | 上海交通大学 | Diluent for sperm of blue fox |
| CN1800369A (en) * | 2005-12-15 | 2006-07-12 | 上海交通大学 | Canine semen dilution liquid |
| CN102823580A (en) * | 2012-08-19 | 2012-12-19 | 四川省畜牧科学研究所 | Diluted preservation liquid for rabbit semen |
| CN102812950A (en) * | 2012-09-07 | 2012-12-12 | 江苏省家禽科学研究所 | Chicken semen diluent as well as preparation method and application thereof |
| CN104012520A (en) * | 2014-05-23 | 2014-09-03 | 山西省生态畜牧产业管理站 | Anti-oxidative cattle frozen semen diluent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李新红等: "甘油浓度对蓝狐精子深低温冷冻保存的影响及冻融前后精子的超微结构", 《动物学研究》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110622958A (en) * | 2016-06-29 | 2019-12-31 | 许红喜 | Animal sperm diluent |
| CN110622958B (en) * | 2016-06-29 | 2022-02-01 | 许红喜 | Animal sperm diluent |
| CN114403131A (en) * | 2022-01-21 | 2022-04-29 | 河北科技师范学院 | Semen cryopreservation liquid and application thereof |
| CN114403131B (en) * | 2022-01-21 | 2023-02-24 | 河北科技师范学院 | Semen cryopreservation liquid and application thereof |
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Application publication date: 20151104 |
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| RJ01 | Rejection of invention patent application after publication |