CN105002290A - Molecular detection kit and application for brassica plant cytoplasmic type - Google Patents
Molecular detection kit and application for brassica plant cytoplasmic type Download PDFInfo
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Abstract
The invention discloses a molecular detection kit and application for a brassica plant cytoplasmic type. The invention provides 12 pairs of primers, a proper primer can be selected to identify the brassica plant especially the cytoplasmic type of cabbage type rape according to the specific condition, and the specific identifiable types are pol CMS cytoplasm, nap cytoplasm, cam cytoplasm, ole cytoplasm, ogu CMS cytoplasm and hau CMS cytoplasm. According to the molecular detection kit and application for the brassica plant cytoplasmic type, SCAR marks are designed and exploited based on peculiar MSS sequences of different cytoplasmic types, and in crossbreeding, the application of the brassica plant cytoplasmic type can identify the cytoplasmic type of the selfing line of the cabbage type rape, guide breeding of the cytoplasmic male sterility line, the maintainer line and the restoring line and can also identify the purity of the sterility line, improve the breeding efficiency and lower the breeding cost.
Description
Technical field
The invention belongs to rape molecular breeding technical field, be specifically related to a kind of molecular detection kit and application of brassica plant cytoplasm type.
Background technology
Heterosis utilization is the main method of rape yield increasing both at home and abroad at present.At present, the main path that rape heterosis utilizes mainly contains method (Fu Tingdong, 2000 such as cytoplasmic male sterility, nuclear male sterility, Self-Incompatibility, chemical emasculation and transgenosis; Official's spring cloud etc., 2012).Cytoplasmic male sterility, as one of the main path of heterosis utilization, Rape-seed production obtains very large application.In the kind that current production uses, more than 60% is the cross-fertilize seed be bred as by cytoplasmic male sterility.The sterile system of pol CMS China three be with temperature sensitive type bilinear method hybrid seeding in apply very wide, apply more (Fu Tingdong, 2000) in ogura CMS (writing a Chinese character in simplified form ogu CMS) rapeseed breeding abroad.In Semen Brassicae campestris production process, the utilization of cytoplasmic male sterility can reduce breeding cost, and hybrid vigour is strong, and the procedure of breeding is simple.
Along with the research of napus hybrid breeding, in rape, find a lot of different kytoplasm type, comprised homology kytoplasm and allo-plasm.Wherein homology kytoplasm has, pol CMS kytoplasm, nap kytoplasm, cam kytoplasm, ole kytoplasm (Fu Tingdong, 2000); Allo-plasm has: ogu CMS kytoplasm, hau CMS kytoplasm, Tour CMS kytoplasm, kos CMS kytoplasm, (Fu Tingdong, 2000 such as Nca CMS kytoplasm, Nsa CMS kytoplasm; Hu et al, 2002; Wan Zhengjie etc., 2006; Wei et al, 2007; Wan et al, 2008).Along with the development of sequencing technologies, in rape, the Mitochondrial Genome Overview of pol CMS kytoplasm, nap kytoplasm, cam kytoplasm, ole kytoplasm, ogu CMS kytoplasm, hau CMS kytoplasm has all checked order (Handa, 2003; Chang et al, 20011; Chen et al, 2011; Tanaka et al, 2012; Wang et al, 2012; Heng et al, 2014).In order to these cytoplasmic regions can be separated molecular level is more effective, be contrasted by Mitochondrial Genome Overview, developing the Mitochondrial DNA molecule marker that different kytoplasm is special, in order to identify the different kytoplasm type of swede type rape rapidly and accurately.The special mark of these kytoplasms not only can be used for the seed selection instructed the analysis of swede type rape Cytoplasm classification, instruct cytoplasmic male sterile line and maintenance line and restorer, and may be used for distinguishing novel kytoplasm type and cytoplasmic male sterile line Purity.
Summary of the invention
The object of the present invention is to provide a kind of molecular detection kit of brassica plant cytoplasm type, this test kit comprises a kind or 2 kinds or 3 kinds or 4 kinds or 5 kinds or 6 kinds or multiple combination in primer MSS-2, MSS-4, MSS-6, MSS-7, MSS-8, MSS-9, MSS-13, MSS-14, MSS-21, MSS-26, MSS-61, MSS-67.
Another object of the present invention there are provided a kind of application of molecular detection kit of brassica plant cytoplasm type, this test kit is Chinese cabbage, for the qualification of kytoplasm type in wild cabbage and swede type rape, distinguish pol CMS kytoplasm, nap kytoplasm, cam kytoplasm, ole kytoplasm, ogu CMS kytoplasm, the kytoplasm type of hau CMS kytoplasm, for the identification of different kytoplasm types and sterile line purity, to accelerate the differentiation of the different kytoplasm type of brassica plant and the discovery of new kytoplasm and the cultivation of new variety, be specially adapted to the Molecular Detection of swede type rape cytoplasm type.
The present invention is achieved through the following technical solutions:
Invention thinking of the present invention is:
Plant the Mitochondrial Genome Overview of turnip type rape cam kytoplasm and wild cabbage ole kytoplasm according to 4 kinds of swede type rape kytoplasms such as pol CMS, nap, ogu CMS and the hau CMS checked order and swede type rape two ancestors, extracted Mitochondrial Genome Overview sequence (the mitotype specific sequences of 90 different kytoplasm type specifics by alignment.MSSs) (Fig. 1), based on these special Mitochondrial Genome Overview sequence nucleotide sequences design primer, exploitation obtains a set of SCAR molecule marker combination distinguished for brassica plant kytoplasm, comprises 12 pairs of SCAR primers altogether.The name of 12 pairs of SCAR mark is called: MSS-2, MSS-4, MSS-6, MSS-7, MSS-8, MSS-9, MSS-13, MSS-14, MSS-21, MSS-26, MSS-61 and MSS-67.Utilize this 12 pairs of SCAR mark, can isolate distinguished sequence fragment from the swede type rape of 6 kinds of different kytoplasms such as pol CMS, nap, ogu CMS, hau CMS, cam, ole, the nucleotide sequence amplified is as shown in sequence table SEQ ID NO:1 ~ NO:12.
A molecular detection kit for brassica plant cytoplasm type, this test kit comprises a kind or 2 kinds or 3 kinds or 4 kinds or 5 kinds or 6 kinds or multiple combination in primer pair shown in table 1.
Table 1 is for the SCAR mark primer sequence of brassica plant cytoplasm type Molecular Detection
Described brassica plant is preferred: swede type rape, turnip type rape, wild cabbage.
An application for the molecular detection kit of brassica plant cytoplasm type, is included in the application in expert evidence pol CMS kytoplasm, nap kytoplasm, cam kytoplasm, ole kytoplasm, ogu CMS kytoplasm or hau CMS kytoplasm; Be included in the application on Cytoplasm classification; Application etc. on application on swede type rape cytoplasmic male sterile line, maintenance line and Breeding for restoration lines and swede type rape cytoplasm male sterility line Purity.
Application process comprises, and utilizes the DNA of the material to be identified of the primer pair in test kit to carry out PCR, and in order to judge the kytoplasm type of this material pol CMS kytoplasm, nap kytoplasm, cam kytoplasm, ole kytoplasm, ogu CMS kytoplasm or hau CMS kytoplasm, decision procedure is as follows:
MSS-2 can amplify band in the material of nap and ogu CMS kytoplasm type;
MSS-4 can amplify band in the material of nap and ogu CMS kytoplasm type;
MSS-6 can amplify band in the material of cam and pol CMS kytoplasm type;
MSS-7 only can amplify band in the material of ole kytoplasm type;
MSS-8 only can amplify band in the material of pol CMS kytoplasm type
MSS-9 only can amplify band in the material of pol CMS kytoplasm type;
MSS-13 only can amplify band in the material of ogu CMS kytoplasm type;
MSS-14 only can amplify band in the material of ogu CMS kytoplasm type
MSS-21 only can amplify band in the material of ogu CMS kytoplasm type;
MSS-26 only can amplify band in the material of hau CMS kytoplasm type;
MSS-61 can amplify band in the material of nap, cam, ole, pol CMS and ogu CMS kytoplasm type;
MSS-67 can amplify band in nap kytoplasm, pol CMS and ogu CMS cytosolic material.
The result of remaining is then other types kytoplasm.
Those skilled in the art can the kytoplasm type of expert evidence according to specific needs, effectively selects above-mentioned primer or combination of primers to identify.
The kytoplasm type of unknown brassica plant material can be identified fast in order to upper mark.
More detailed technical scheme is as described in " embodiment ".
Positively effect of the present invention
1. plant the special Mitochondrial Genome Overview sequence of kytoplasm of turnip type rape and wild cabbage based on 6 kinds of different swede type rapes such as pol CMS, nap, cam, ole, ogu CMS and the hau CMS checked order and ancestors thereof, design and develop the 12 pairs of SCAR mark combinations obtaining distinguishing for swede type rape kytoplasm, differentiating.
2. provided by the invention 12 can be used for swede type rape Cytoplasm classification, swede type rape cytoplasmic male sterile line, maintenance line and Breeding for restoration lines to the SCAR mark combination that special MSS is correlated with, and swede type rape cytoplasm male sterility line Purity.
Accompanying drawing explanation
Fig. 1 is the visual figure of the MSS sequence that in the swede type rape of 6 kinds of different kytoplasm types, kytoplasm is special.
6 kinds of different kytoplasm type pcr amplification situation schematic diagram in 12 pairs of primer pair swede type rapes in Fig. 2 embodiment 1.
Embodiment
Below in conjunction with specific embodiment, illustrate the present invention further.These examples of implementation are only not used in for illustration of the present invention and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usual conveniently condition is as " molecular cloning: lab guide " (New York:Cold Spring Harbor Laboratory, 1989) condition described in, or the method for advising in the operational manual provided according to manufacturer.Experiment material of the present invention, if not otherwise specified, is commercially available.
Embodiment 1:
The exploitation of the molecular detection kit primer of brassica plant cytoplasm type and checking:
The exploitation of 1.1 different kytoplasm type specific marks
1) first at GeneBank, pol CMS kytoplasm Mitochondrial Genome Overview sequence (sequence number: FR715249) will checked order in EMBL and DDBJ tri-large database concept, ogu CMS kytoplasm Mitochondrial Genome Overview sequence (sequence number: AB694744), hau CMS kytoplasm Mitochondrial Genome Overview sequence (sequence number: KF736092), nap kytoplasm Mitochondrial Genome Overview sequence (sequence number: AP006444), cam kytoplasm Mitochondrial Genome Overview sequence (sequence number: JF920285), ole kytoplasm Mitochondrial Genome Overview sequence (sequence number: JF920286) extracts.
2) utilize Progressive Mauve and the sequence of BLASTN localization software to these 6 Mitochondrial Genome Overview to be analyzed (e-value:1e-5), the sequence bit dot information of collinearity in the Mitochondrial Genome Overview of these six kinds of kytoplasm types and different kytoplasm type specific is analyzed.According to sequence bit dot information, Mitochondrial Genome Overview sequence special for each kytoplasm is extracted.From these six kinds different kytoplasm plastosomes, altogether obtain the sequence MSSs (Mitotype Specisic Sequences) of 90 different kytoplasm type specifics: the sequence (from MSS1 to MSS5) that 5 nap kytoplasms are special; Article 1, the sequence (MSS7) that ole kytoplasm is special; Article 2, the sequence (MSS8 and MSS9) that pol CMS kytoplasm is special; Article 13, the sequence (from MSS10 to MSS22) that ogu CMS kytoplasm is special; Article 9, the sequence (from MSS23 to MSS31) that hau CMS kytoplasm is special; Article 1, cam kytoplasm and the jointly special sequence (MSS6) of pol CMS kytoplasm; And 59 two or more five kinds of special MSS sequences (from MSS32 to MSS90) had with endoplasm.
3) according to the MSS-SCAR primer of the different kytoplasm type specific of the sequences Design extracted.
Therefrom choose 12 sequences at the not conservative section design primer of sequence, the primer sequence designed is in table 1.
1.2 utilize the material of the known different kytoplasm type of below the primer pair that designs to carry out pcr amplification checking
The material of nap kytoplasm: in two No. 11, middle oily 821 and swede type rape Wester self-mating system (Handa, 2003);
The material of cam kytoplasm: triumph rape, salt oil No. 2 and breeds of Chinese cabbage Suzhongqing (Chang et al, 2011);
The material of ole kytoplasm: wild cabbage self-mating system 08C717 (Chang et al, 2011), ole kytoplasm swede type rape;
The material of pol CMS kytoplasm: China's oil assorted 62 and swede type rape sterile line 1141A (Wan et al, 2008);
The material of ogu CMS kytoplasm: river oil 41 and swede type rape sterile line 6-300A (Wan et al, 2008);
The material of hau CMS kytoplasm type: 6W-301A (Wan et al, 2008), swede type rape sterile line 00-6-102A (Wan et al, 2008).
Pcr amplification program: 94 DEG C of total sex change 5min; 94 DEG C of sex change 30S, 55 DEG C of annealing 30S, 72 DEG C extend 1min, 25cycles; 72 DEG C extend 10min.Amplification after product is detected by agarose gel electrophoresis, and in swede type rape, 6 kinds of different kytoplasm type pcr amplification situation results are as Fig. 2.
MSS-2 can amplify band in the material of nap and ogu CMS kytoplasm type;
MSS-4 can amplify band in the material of nap and ogu CMS kytoplasm type;
MSS-6 can amplify band in the material of cam and pol CMS kytoplasm type;
MSS-7 only can amplify band in the material of ole kytoplasm type;
MSS-8 only can amplify band in the material of pol CMS kytoplasm type
MSS-9 only can amplify band in the material of pol CMS kytoplasm type;
MSS-13 only can amplify band in the material of ogu CMS kytoplasm type;
MSS-14 only can amplify band in the material of ogu CMS kytoplasm type
MSS-21 only can amplify band in the material of ogu CMS kytoplasm type;
MSS-26 only can amplify band in the material of hau CMS kytoplasm type;
MSS-61 can amplify band in the material of nap, cam, ole, pol CMS and ogu CMS kytoplasm type;
MSS-67 can amplify band in nap kytoplasm, pol CMS and ogu CMS kytoplasm.
The kytoplasm type that those skilled in the art identify according to specific needs, effectively selects above-mentioned primer or combination of primers to identify.
Because swede type rape is the allotrtraploid formed by turnip type rape and wild cabbage hybridization Natural double.Therefore, the mark of above-mentioned exploitation can also be used for the qualification work of the kytoplasm type of turnip type rape and wild cabbage.Experimental result confirms simultaneously, and the wild cabbage (as ole kytoplasm) that kytoplasm type is identical with swede type rape or turnip type rape (as cam kytoplasm), the amplification of 12 pairs of primers is also identical with swede type rape.
Embodiment 2:
The qualification of the kytoplasm type of 570 parts of swede type rape self-mating systems:
From in pair MSS-SCAR labeled primer of 12 embodiment 1,6 couple mark (MSS-4, MSS-6, MSS-7, MSS-8, MSS-13, MSS-26) the kytoplasm type to 570 parts of swede type rape self-mating systems chosen wherein is identified.
Pcr amplification program: 94 DEG C of total sex change 5min; 94 DEG C of sex change 30S, 55 DEG C of annealing 30S, 72 DEG C extend 1min, 25cycles; 72 DEG C extend 10min.Then add up the kytoplasm type of each swede type rape self-mating system, the decision method of kytoplasm type is as follows:
Only MSS-7 can amplify band, illustrates that the kytoplasm type of this material is ole kytoplasm;
Only MSS-8 and MSS-6 can amplify band, illustrates that the kytoplasm type of this material is pol CMS kytoplasm;
Only MSS-13 can amplify band, illustrates that the kytoplasm type of this material is ogu CMS kytoplasm;
Only MSS-26 can amplify band, illustrates that the kytoplasm type of this material is hau CMS kytoplasm;
Only MSS-6 can amplify band, and MSS-8 can not amplify band simultaneously, illustrates that the kytoplasm type of this material is cam kytoplasm;
Only MSS-4 can amplify band, and MSS-13 can not amplify band simultaneously, illustrates that the kytoplasm type of this material is nap kytoplasm.
Statistics is in table 2, wherein the self-mating system of nap kytoplasm and pol CMS kytoplasm type is maximum, account for 80% more than of 570 parts of swede type rape self-mating system kytoplasm types of qualification, and cam kytoplasm, ogu CMS kytoplasm, the ratio that ole kytoplasm and hau CMS kytoplasm account for is less, illustrates that they also have very large development space in breeding.In addition, also have the kytoplasm type of 37 parts of materials not to be detected, illustrate that the self-mating system of these swede type rapes may be some new not certified kytoplasm types, need qualification further, exploitation, utilize.
Table 2 is to 570 parts of qualification results of 570 parts of swede type rape self-mating systems from each breeding units of the whole nation
Embodiment 3:
Be known as the material qualification of nap kytoplasm, pol CMS kytoplasm, ogu CMS kytoplasm and hau CMS kytoplasm type:
Material used is:
In embodiment 2 identify into nap kytoplasm, pol CMS kytoplasm, ogu CMS kytoplasm and hau CMS kytoplasm type material and in two No. 11, China's oil assorted 62, river oil 41,6W-301A.
Primer MSS-2, MSS-14, MSS-21, MSS-61, MSS-67 in embodiment 1 is utilized to increase to above-mentioned materials, PCR decision method according to embodiment 1, MSS-2, MSS-61, MSS-67 all have band, and MSS-14, MSS-21 all without band be nap CMS kytoplasm type; What MSS-61, MSS-67 all had band is pol CMS kytoplasm type; What MSS-2, MSS-14, MSS-21, MSS-61, MSS-67 all had band is ogu CMS kytoplasm type; MSS-2, MSS-14, MSS-21, MSS-61, MSS-67 all without band be hau CMS kytoplasm type.Net result and reality completely the same.
Embodiment 4:
Be known as the material qualification of pol CMS kytoplasm type:
Material used is:
In embodiment 2 identify into nap kytoplasm, pol CMS kytoplasm, ogu CMS kytoplasm and hau CMS kytoplasm type material and in two No. 11, China's oil assorted 62, river oil 41,6W-301A.
Utilize the primer MSS-9 in embodiment 1 to increase to above-mentioned materials, the PCR decision method according to embodiment 1, what have band is pol CMS kytoplasm type, and other cytosolic material are all without amplified production.Net result and reality completely the same.
SEQUENCE LISTING
<110> Hua Zhong Agriculture University
The molecular detection kit of a <120> brassica plant cytoplasm type and application
The molecular detection kit of a <130> brassica plant cytoplasm type and application
<160> 36
<170> PatentIn version 3.1
<210> 1
<211> 560
<212> DNA
<213> swede type rape
<400> 1
ctgaagttga aaaagggaat gtaaatgcca acattgcatt ccaattccaa gttggtcgtc 60
cctacaagac gaaatcattt ctgctttgtt acgattgatc aaaagaggag ctcctcaagg 120
cattcaaaag aagactcttc tggagaagtc tcattaacta gttagttgat gaggatttga 180
agggtcagaa aaaagccttt caccaagagt ccttcttatc cccttcacga cctgcagatc 240
ctctaaaaca ttcatcatat gattatagga gcgtcatacc tgacttcggg ggtcgcagtc 300
gaagtcaaga tcgtaccagt ccgaacaacc cttgggtttt gatataatta gaatctagtc 360
cgcagtaggc ttgtgctcgg cttatgattt tgactagcaa ccgctattaa aagaatctgc 420
tccgccgtgt agtccaatcg ggatctttga ctcttgactt atatattcaa agaaaagccg 480
ccccccgaat cgaaggacac cttaaagtac acgacagtct ggctcttttt tctttcggag 540
ctactcctcg tcttgatcaa 560
<210> 2
<211> 474
<212> DNA
<213> swede type rape
<400> 2
agtatttcgt tcaccttggc tctcttcgaa tctcgcagac ggtctaggta ccgaaagacc 60
cggaagtggt gatccaagct cctgacctcc cacttcatga tatcgacgag ggcttgacgc 120
cgcgcgatat catgctcacc ctctaggtca cgggttatct cttgcactcg gctaatatgc 180
gctagcatat cagcattccg cgcttgttga gagtcggaac tctcaacatc cgcgtacaaa 240
agatgcagtg gcgggcgctc gttgagatct agagctgggg gcgcgacgcc atctggtccg 300
acgccgaact gaaatatctc atttcctagc aacccccatt taatcccaaa aataaaattc 360
aaaccatata ttagatatat agctagccgc atttttggga ccacgaacat gaggaaatag 420
cgcgtggcga acgctaagac cccgaactta cttgtacgat cttttccacc aagc 474
<210> 3
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<213> swede type rape
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cgtcatctca tatcatcaga taaaacgact attaatttca agtcagtcag aatttgcggg 180
atcttgactg tgaggagcaa gaaaagaaag aagaactcat caggcaagaa gggacttttc 240
actctttcgg cgatgtaata gaagatgcag acttcgggca ttcaaatgac ttcctgttct 300
ggaatgtcaa tccattagta gtagatgtcg gcatttccta aagaaagctg tttgagtagc 360
aggaggaccc ggcggagcgc taatgaataa tcgaaggcgg attaagagaa gagagctgtt 420
agcgtagatg aaagttgcgg gttagtgctc caatatcctc agtagataga ggtagaagaa 480
cagctataac agtatacata gtaggtagcg agatcaaacc tagtgcgtca ctatcatctt 540
acgcaccgat cggtctatct gatcaggttc agtatccaat cctgtgtagg gcgggggagc 600
tgctagttcg ctaggaaaat agaaaaagaa tatagaaaat ctataatcag tcaattgcaa 660
ttggagaata aagttagata tacttctcgc taatccttct tcttttgatc agcctagcat 720
ctcctcgtgc ttatttattc tttcactttg acctactgca agacacatgc ccatcccaag 780
agcgctatga attcatagag tggagaattc ctcatggttc atcctttttt catgcaaggc 840
tagtacagat ggtgagaatg ctctggaaga aggtgtccca gtccgtaggg gcactttcaa 900
tcaacccggt gggcgctcag tgactcgact gaaacgcact ttagtgacta gagaaagaag 960
ttctctagga gaaggtactc tcttacttat tggtccaacg gaattctgct ttatcatgaa 1020
agttctcgac cgaggaattc catttcggta gcaggcttct aaatacgctg cttgcccgaa 1080
ccaaggacct tcacattcta atcaaaggta agatgctaag ggtaccgata acttctgatt 1140
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tggttaaggc aaggccttaa aacatcgatc aagcattcta tcgagccctt actcatcaat 1260
caatgaaaag agataccgac cctcttgtgg attgtgcgtg aggagcctcc ctatcaaaac 1320
aattagtctc ttccaatgag cagaaaaaca acgataggca attaccccat gctactctca 1380
gccagatg 1388
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<213> swede type rape
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cccccatcta ttcactccga ggaacgcctg ttgttagcca accaatccca tgagctagct 60
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aaaggaaaga tcgtaaaagt agcgtagaac cctctccctt cgggtgtact tgtcctatcg 180
aacaaacttc ctgtaaggga ggaatttatt tctattctat aatacagaga aagaaagcgg 240
gaaagaaaac tcactgggga gacgcttgat tcgctgcaaa tcagagtgta agaaagcaaa 300
gaagagaagt cgacaaataa gctgctagtg cttgagtaag cggcgtgaga gtatccagtc 360
ctgggggaag agaagctcta agcgtacccg cagttcacgt accagctctt agaaatgttc 420
acgtatccag tgctagtctt ttctgttcca gtaagagtag ttggaggact agctagtgaa 480
tcagctgtta ggggcaaggc cgcttgacca acgggagaag ccaactccgt tcctcctaac 540
acagatatca gatattaggc ttggcaacag acgctttgat atgtgttaca cagacgccct 600
gtagacattg aatcggctca ctccgacttc gattgctaaa atgttgacgt gaatacgctt 660
gctggttgtg atatgttccc tactatacta ctattgaaga ccttgacttt tgatcttcct 720
tctaggagaa ttcatcaaac cttgcttatc cttctgatga gcaagctttt cctctaacta 780
agttagactg cctcttacga gcaggtaacc taccttctaa accatgcccc actatacttt 840
cttctaagcc agcttttcac tctttgtttt cgtatcatct cattggactt atttcaaaca 900
aactcgttac actcgcacct gatacacggg aacgaagaaa actacatggg gagcacgctt 960
acacctgcaa gaactaaggg tccagaccaa gcacagacag at 1002
<210> 5
<211> 599
<212> DNA
<213> swede type rape
<400> 5
gagaaagaga gaaggttgct gcgctaggag agtgttgaag ctatagattg atttagtaga 60
gactaggagt ctctaactta ctgtgctttg gccgagggtg aagtcgaaag atatatcaga 120
gggctgccct ttcttcttaa gtcaaataaa gctcctcaaa actgacctga tctattgttt 180
gagtctaacc gagaagtctc atcgctttga gcctggttta ggcgcatacg cccatagagt 240
agcccagccc gaccaatcat cagtgtacgt ggtccaagga aatcaaggct ttatggccaa 300
tgaagaggtg ggctatgaag gaatggaaaa gcagggttgt gcttgtgctg cagtttagaa 360
tgctgattcc tgggtgggta gttcctactc ttgcttccct tggttttagc aggtatttgt 420
ttctagcttc ggtagttaac caactattcc cttttcagca cactctttgg cgtgcgataa 480
tagtaaactt ttaggaatcc cacaccaagt gaacagagtt atcggctgat actttccttg 540
ttcctagggt tgagcagaag ttggttgact agctactgaa gtcagcacac tcctctgct 599
<210> 6
<211> 656
<212> DNA
<213> swede type rape
<400> 6
ggatctcgtt caccttggct ctctgcgaat ctcgcagacg atcaaggtgc cgaaagatcc 60
gaaagtgctc ctgaaggctt ctgacctccc atttcattat atccaggagg gcttgacgcc 120
gcacgatatc gcgctcaccc tctagttttt gggtgatctc ctgtacgcgc ctaagatgcg 180
cgtacatgtc attatttcgc gcttgttgag agtcggaact ctcaacatcc gcgtacaaaa 240
gatgcagagg cgggcgctcg ttgagatcca gcgctggggg cgcgacgcca tctggtccga 300
cgccgaaatg acaaatttca tttcctaata ccccccattt caaccccaac aaacaaaaaa 360
aattatatat aatgtagaaa actggtccca attttgggac cacgaaaatg ataaaataat 420
gggcggccaa cgctgatacc tcgaaccgac ttgaatctga actacgattt tttccaagcc 480
ttcccttgct caggagggtc ttcccccggt gtgaaagcag ttggttccat agttttagaa 540
ttctgctgat cccaagtact ccatctccat cattgcatat gaaaatatag aaagtaaaga 600
agaaaaggca taaccagaag aattgtgaaa aataagtgaa tttatccagt tgaggc 656
<210> 7
<211> 1307
<212> DNA
<213> swede type rape
<400> 7
tagcgctatc tttcggccct ctctcggttt gtagcgcgta agatatagta cttcaagacc 60
caaccagtac cgaacctttc ttatggtccc atttccgtgc aactctatgg aatcgtagta 120
gcatagctgc attctcttcg tatttagatc tggaaagctg ctcccccttg cccgggatca 180
ctttcacttc tgccgacgtt taccgttaag actggtgcct tccgttctag aaaggaaagg 240
tttggattcc tactgtcctc ttacgaagga aaataggatt gctaactttt cctgatatat 300
aaagagaaag tttgcattct aggaaagggc tcggactcct cttctctcag atcactcggt 360
gctgaagtgg cacgaccatt tgtgaaacat ggtaatctag atcccctact gcgtcggtgg 420
attctagaac tccatcccga gatccgacac tccgtcatgg gtttgatgtc tcgattcagg 480
cggagagagc tggctcgaaa gaggagccat ggactggacc agatctgata gagcttggga 540
agaaactgac ctcctgaaca ggtctggtgg agacaaacgg attcgaaaca caggggtgat 600
agcagaatgg agacagttag cttcacagaa caggattaca tggagtaata aggatccctc 660
tcagctggta gagaacgaga caggagagtg aatactgggt ataagatttc gggctgctca 720
gggacagtcc tggaaaaatg gatcaaccat ctggaatcat atttctggga gtatcacatc 780
caagagaatc agagagagcc ggttgtgtac ctctgtctcg agggaccaac agggatttgg 840
atttcggaac tgagggatag agatccacca aacagttggg aagagcttaa acagaggcta 900
cgacgagggg cagatgcgat ggcaaaggaa accaatcagc ctcggtattt tggaattcgt 960
caggatgggt ctgtccgtga gtataggaaa aggtttgaag ctctctgtct taagactctt 1020
agcttgccaa ggaaatcttt tgaggaggtt ttcttgctgg ggttgaagcc gcagctacaa 1080
gcggcagttc gtcggctacg ccgcttcaat gacaggatca ggcccaaaag tccaaggggt 1140
ggggtcagcc ggaagaggaa ggtatagcca caacacatag aagaacccca cttacagcta 1200
atgactacac agaaatcttt ctgcggaacc cttgctgaat agcttaccag ccacttgtca 1260
agactgccaa atggtgaagc tgctaaaaga gaccaggacc ttcgggt 1307
<210> 8
<211> 317
<212> DNA
<213> swede type rape
<400> 8
gctcgttcga ttaagctcaa aaagcatttg ccggaacaag agtgaattca tctctttttt 60
tcatcgacgc aaggcaactc tgttgaatca atgccctagt gatttaggaa atggagatct 120
gtctgaagcc tacgatcagg ggaaagagag atctaaagtc aaagaaagct ctagtcatgc 180
tagttttcta tgatcccaag tcaagatcat ccttcagtca acattctact accggttatt 240
ttataactca atcaaagaag agagcgatcc aggggtctag ttgcaaatag gtatcacccg 300
caatgaaaga ggaattc 317
<210> 9
<211> 970
<212> DNA
<213> swede type rape
<400> 9
ggattatcct gtggtgtggc tatctgctgc ttcagattca tttcattccc tcccctcaac 60
tgattatgat tgcgaggccc cctataagat aagacttagg ttatctattc ctccttgcag 120
gtccaagcgc aaggcgaaca atgcctatct ttgggatcct agctttgcag cagggggcca 180
aaaccaccgt ttaactagct ctttcgacat cctgccggga gtatgaatcc gtattaagct 240
aaggctttca atccaaagca ttgaagaaag aagaaactcc gagtggaagg ttcaaggatt 300
attctgattg cacgagagat tgccctacca gctaactcct attgacctta ctaaaacaaa 360
agcaccaaga cttatactag ctaacacaga agagacacca taggaaaggg cacaccaaac 420
cattttttca caaggtcacc ctcaaccact gtcactttga tcaatacctt gctttactgt 480
aaaagatcct tacacatggt ttcttcctag aacacacagt gccagaactt atgacacata 540
cctgcttcca tcaaccacat cttgcaatat ttcacaccac ataaacaacc aagaaatact 600
tctttctttg cttgtttacc ttattcccaa aacgcaactc atatggcttc acaataaacc 660
attggttcca agagtcttcc ttcctcttat gttgtaagag catgaactac cccactgttt 720
tcttcaaact tctgtgcagg tcgagtctcc cttcttgttc tttccatggg aagatgacaa 780
tctgccaaac ccacaagatc tcgatgaatc aacctctgag tcagccactt caacaccagt 840
ttcagacttc tccaatcctc tatgagaatc accagtaacc ctgtttacgc cacctgagac 900
aagagttcct tgttctccca gatttcctgt aaactgacaa caaattttgt tccaacaaac 960
ccaccatgtg 970
<210> 10
<211> 1401
<212> DNA
<213> swede type rape
<400> 10
gagtagttta gcggctgcct tctaattcct aggttcttac ctgtttcgta aaccaggact 60
cttatccagc acactagggt gcctgttttc ctcttctgct atttcctgtt ttcctgttct 120
gctaatagta cgcaagtcaa ggtcttctgt tttgtgtgca attaaatccc atttgacaaa 180
actcttagat actcagcaca gaccttcaag ggttttctca actcgatttc ttaacagaag 240
aatggccata caaagaggat tggtatgttt cgaaatgtga cagaaaagcc aatctaatag 300
gcattcctac tataagagta gtgtgctgga gttgagctct ttgtttaaga taggaatccc 360
aggagttcag cagcacatca atcgattaaa ggcagataaa aggcatctga gatgagatgg 420
ataagccagg ctagtatgtt ttggtaatag cacactgctg tgaagctggt ttgtagttct 480
tagactagct agggagtaat gcgttagccg tattcctagt ggagcacact gatgtacgag 540
acaggggtag gaagattcct ccctaacttc tgatctgtaa ctagaactct ttttttgttg 600
gcggtgtgct gcaggtgagt tccttgttct ttctagtatg gagtaaacct tgtcctattc 660
cttttcatag gtaggtaaaa agtgaagggt tcctctttta ggcttttaag gcagctaatt 720
gctgaggtgg gagttgaaca aatagggaga cttatctgaa ttcctaggta tgggcagcta 780
gagctagtaa gggttgtaat agctagggag ttagcagccc taagagctgc attcctaagt 840
atgggttggt ccagcacacc gcagtgtgtt ggaagctctg tttgtttcct ttcctaatag 900
ctctgtttgt ttcctttcag cacacttagc taaagagtta tctctagtat tcagttgtgt 960
gctgaagatg aatccctaag gctagtactt ctatccagca caccgaaggg tagttctgtt 1020
cctttctttc agccagtctt ataatctgac tatctatgtt acccctagta ataagttgta 1080
agattactcc cacgggatgc agcacaccgg tgtgctctct tcatctttca taggtagctt 1140
tataagagta ctagctaggg agttagcctt ggcagtattc ctaagtatag atgtgggaat 1200
gttctaagcg atagtaagat gacataggtg cccggccagc acaccgctgc tttagtgagg 1260
ctggaaactc tttcctttcc tcttccttag attctttaga agctggtatt ttcagaagta 1320
gtagaattga attctttagc tagggtaagg gtttgttttc cttggttttc caatgaatac 1380
catttctctg ctttggtatg a 1401
<210> 11
<211> 607
<212> DNA
<213> swede type rape
<400> 11
ctttccgcag gcaactgttc cgaccaggaa ggtgaacccc tccgctgatc gttagagcgc 60
tcattatcaa aatagctgct aagaaaaccg tcgaatgggt ttgtaggggg cataggcgta 120
tttccccccc ccataaatcc tgatatcggc gggtagaatt gagaaatggc acgagtcaaa 180
tcgtcgaagc ataagtaacc cactgaaaat aataatttta tcataataat ggaaaaaatg 240
aatttgaaaa tagaaaacca aaggaattgc catttcattg acaatttcct tataatagga 300
aatgacgaaa agtaagtgaa aaaggtttct atgctgcaaa agactgggat gggaaacaaa 360
acggaatgta caaaatttat agcacgtgta accaataaac caaagactaa agcagggctc 420
gacaaaacag aaagtatcat gggtacgtcg tccttccctg aaatggaact ttcatgctag 480
ttcttgcttc agcatgaaag tcgatctatt acgaccagcg cggttgttcc tatttcattt 540
tcttcttcct tgcccttctt tcttagaaag cactcactga gttacttacg gaatctcaat 600
gcagaaa 607
<210> 12
<211> 468
<212> DNA
<213> swede type rape
<400> 12
aagcaaagca gcaccaacct agctctactc tagctaatca atcatggcct acttctaact 60
gtaaatgtga tcactagcat cacaaaaaca acagaagcgg tatcgaaata agcacctgtc 120
ccaactcaag cctgatatcc ctggcacaac agagtttgag acttcgccgg ataagcccga 180
tattgagcct atctgactga gccttagcca gagatgaagg gcttggcacc accctaacgc 240
agtaaatgaa gcgatatccc ttgagcgaaa gctttatttt gggcatctac aggagaagtc 300
gcttatggaa agaaacagaa gcctagctgc taacaaagac ctatgcggct tgcttggggt 360
agtgggcagg catttaatcg agaaagaagg ctcttgagta aacagagtca tcatatgaga 420
cttctgtcgg agtgctgtgg aaagactttc gtccgcataa gcaaagat 468
<210> 13
<211> 22
<212> DNA
<213> artificial sequence
<400> 13
ctgaagttga aaaagggaat gt 22
<210> 14
<211> 22
<212> DNA
<213> artificial sequence
<400> 14
ttgatcaaga cgaggagtag ct 22
<210> 15
<211> 20
<212> DNA
<213> artificial sequence
<400> 15
agtatttcgt tcaccttggc 20
<210> 16
<211> 20
<212> DNA
<213> artificial sequence
<400> 16
gcttggtgga aaagatcgta 20
<210> 17
<211> 20
<212> DNA
<213> artificial sequence
<400> 17
cagagggcaa aactgtatga 20
<210> 18
<211> 22
<212> DNA
<213> artificial sequence
<400> 18
catctggctg agagtagcat gg 22
<210> 19
<211> 29
<212> DNA
<213> artificial sequence
<400> 19
ctagtgaatc agctgttagg ggcaaggtc 29
<210> 20
<211> 32
<212> DNA
<213> artificial sequence
<400> 20
atagtggggc atggtttaga aggtaggtta cc 32
<210> 21
<211> 20
<212> DNA
<213> artificial sequence
<400> 21
gagaaagaga gaaggttgct 20
<210> 22
<211> 20
<212> DNA
<213> artificial sequence
<400> 22
agcagaggag tgtgctgact 20
<210> 23
<211> 20
<212> DNA
<213> artificial sequence
<400> 23
ggatctcgtt caccttggct 20
<210> 24
<211> 20
<212> DNA
<213> artificial sequence
<400> 24
gcctcaactg gataaattca 20
<210> 25
<211> 20
<212> DNA
<213> artificial sequence
<400> 25
tagcgctatc tttcggccct 20
<210> 26
<211> 20
<212> DNA
<213> artificial sequence
<400> 26
acccgaaggt cctggtctct 20
<210> 27
<211> 20
<212> DNA
<213> artificial sequence
<400> 27
gctcgttcga ttaagctcaa 20
<210> 28
<211> 20
<212> DNA
<213> artificial sequence
<400> 28
gaattcctct ttcattgcgg 20
<210> 29
<211> 20
<212> DNA
<213> artificial sequence
<400> 29
ggattatcct gtggtgtggc 20
<210> 30
<211> 20
<212> DNA
<213> artificial sequence
<400> 30
cacatggtgg gtttgttgga 20
<210> 31
<211> 20
<212> DNA
<213> artificial sequence
<400> 31
gagtagttta gcggctgcct 20
<210> 32
<211> 20
<212> DNA
<213> artificial sequence
<400> 32
tcataccaaa gcagagaaat 20
<210> 33
<211> 20
<212> DNA
<213> artificial sequence
<400> 33
ctttccgcag gcaactgttc 20
<210> 34
<211> 20
<212> DNA
<213> artificial sequence
<400> 34
tttctgcatt gagattccgt 20
<210> 35
<211> 20
<212> DNA
<213> artificial sequence
<400> 35
aagcaaagca gcaccaacct 20
<210> 36
<211> 20
<212> DNA
<213> artificial sequence
<400> 36
atctttgctt atgcggacga 20
Claims (7)
1. a molecular detection kit for brassica plant cytoplasm type, this test kit comprises a kind or 2 kinds or 3 kinds or 4 kinds or 5 kinds or 6 kinds or multiple combination in primer MSS-2, MSS-4, MSS-6, MSS-7, MSS-8, MSS-9, MSS-13, MSS-14, MSS-21, MSS-26, MSS-61, MSS-67;
Described primer is as follows:
2. molecular detection kit according to claim 1, described test kit comprises primer MSS-4, MSS-6, MSS-7, MSS-8, MSS-13 and MSS-26.
3. molecular detection kit according to claim 1, described test kit comprises primer MSS-2, MSS-14, MSS-21, MSS-61, MSS-67.
4. molecular detection kit according to claim 1, described test kit comprises primer MSS-9.
5. molecular detection kit according to claim 1 is differentiating the application in brassica plant kytoplasm type; Described brassica plant is wild cabbage, swede type rape or turnip type rape.
6. the application of molecular detection kit according to claim 1 on swede type rape cytoplasmic male sterile line, maintenance line and Breeding for restoration lines.
7. molecular detection kit according to claim 1 is in the application on swede type rape cytoplasm male sterility line Purity.
Priority Applications (1)
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Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510477044.8A CN105002290B (en) | 2015-08-06 | 2015-08-06 | A kind of molecular detection kit of brassica plant cytoplasm type and application |
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| CN105002290A true CN105002290A (en) | 2015-10-28 |
| CN105002290B CN105002290B (en) | 2017-12-22 |
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| CN201510477044.8A Expired - Fee Related CN105002290B (en) | 2015-08-06 | 2015-08-06 | A kind of molecular detection kit of brassica plant cytoplasm type and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913800A (en) * | 2018-07-24 | 2018-11-30 | 信阳师范学院 | A kind of Chinese cabbage hau CMS sterile cytoplasm specific molecular marker and its application |
| CN111154908A (en) * | 2020-01-20 | 2020-05-15 | 中国农业科学院油料作物研究所 | KASP labeled primer for identifying Brassica crop Cam and Pol cytoplasm types and application thereof |
| CN114350655A (en) * | 2017-08-24 | 2022-04-15 | 湖南省作物研究所 | Exogenous radish fragment specific marker and preparation method and application thereof |
| CN114561485A (en) * | 2022-03-19 | 2022-05-31 | 陕西省杂交油菜研究中心 | Molecular marker group for identifying cabbage type rape cytoplasm three lines and method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1849879A (en) * | 2006-06-05 | 2006-10-25 | 华中农业大学 | Selective breeding method preserved sichuan pickle cytoplasm male sterile line |
| CN101608198A (en) * | 2009-04-30 | 2009-12-23 | 西北农林科技大学 | Method for establishing rape cytoplasm type specific PCR (polymerase chain reaction) marker and rapidly identifying rape cytoplasm type |
| CN102071250A (en) * | 2010-06-25 | 2011-05-25 | 温州科技职业学院 | Broccoli Ogu cytoplasm quick identification kit and detection method thereof |
-
2015
- 2015-08-06 CN CN201510477044.8A patent/CN105002290B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1849879A (en) * | 2006-06-05 | 2006-10-25 | 华中农业大学 | Selective breeding method preserved sichuan pickle cytoplasm male sterile line |
| CN101608198A (en) * | 2009-04-30 | 2009-12-23 | 西北农林科技大学 | Method for establishing rape cytoplasm type specific PCR (polymerase chain reaction) marker and rapidly identifying rape cytoplasm type |
| CN102071250A (en) * | 2010-06-25 | 2011-05-25 | 温州科技职业学院 | Broccoli Ogu cytoplasm quick identification kit and detection method thereof |
Non-Patent Citations (2)
| Title |
|---|
| SHUANGPING HENG ET AL: "Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in Brassica napus L.", 《PLANT CELL REP》 * |
| 衡双平: "油菜hau CMS线粒体基因组和不育基因的研究", 《中国博士学位论文全文数据库》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350655A (en) * | 2017-08-24 | 2022-04-15 | 湖南省作物研究所 | Exogenous radish fragment specific marker and preparation method and application thereof |
| CN114350655B (en) * | 2017-08-24 | 2023-09-26 | 湖南省作物研究所 | Exogenous radish fragment specific marker and preparation method and application thereof |
| CN108913800A (en) * | 2018-07-24 | 2018-11-30 | 信阳师范学院 | A kind of Chinese cabbage hau CMS sterile cytoplasm specific molecular marker and its application |
| CN108913800B (en) * | 2018-07-24 | 2021-12-28 | 信阳师范学院 | Chinese cabbage hau CMS sterile cytoplasm specific molecular marker and application thereof |
| CN111154908A (en) * | 2020-01-20 | 2020-05-15 | 中国农业科学院油料作物研究所 | KASP labeled primer for identifying Brassica crop Cam and Pol cytoplasm types and application thereof |
| CN114561485A (en) * | 2022-03-19 | 2022-05-31 | 陕西省杂交油菜研究中心 | Molecular marker group for identifying cabbage type rape cytoplasm three lines and method and application thereof |
| CN114561485B (en) * | 2022-03-19 | 2023-09-29 | 陕西省杂交油菜研究中心 | Molecular marker group for identifying brassica napus cytoplasm three lines, and method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105002290B (en) | 2017-12-22 |
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