CN105000673A - Method used for treating sorbic acid wastewater with Trichosporoncutaneum - Google Patents
Method used for treating sorbic acid wastewater with Trichosporoncutaneum Download PDFInfo
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- CN105000673A CN105000673A CN201510383950.1A CN201510383950A CN105000673A CN 105000673 A CN105000673 A CN 105000673A CN 201510383950 A CN201510383950 A CN 201510383950A CN 105000673 A CN105000673 A CN 105000673A
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- sorbic acid
- acid wastewater
- cortex
- trichosporon
- wastewater treatment
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Abstract
The invention discloses a method used for treating sorbic acid wastewater with Trichosporoncutaneum, and belongs to the technical field of microbial technology and environmental engineering. The method is used for treating sorbic acid wastewater with biotechnology, obtaining microbial oils, reducing COD of sorbic acid wastewater at the same time, and realizing comprehensive utilization and purifying of sorbic acid wastewater with Trichosporoncutaneum. The method comprises following steps: Rhodococcuserythropolis is adopted to prepare a microbial flocculant; a Trichosporoncutaneum culture solution is prepared; the microbial flocculant is adopted for pretreatment of sorbic acid wastewater; and Trichosporoncutaneum with a strain screening number of CGMCC No:2.0571 is adopted to treat sorbic acid wastewater. According to the preparation method, Rhodococcuserythropolis is adopted to prepare the microbial flocculant, the microbial flocculant is adopted for pretreatment of sorbic acid wastewater, and Trichosporoncutaneum is adopted to treat sorbic acid wastewater, so that microbial oils are obtained, COD of sorbic acid wastewater is reduced, and comprehensive utilization and purifying of sorbic acid wastewater is realized.
Description
Technical field
The invention belongs to microbial technique and field of environment engineering technology.
Background technology
Sorbic Acid is a kind of safety, efficiently food antiseptic, preservation agent, is widely used in the industries such as beverage, food, makeup at present.Can produce a large amount of waste water in Sorbic Acid production process, waste water is in acid, and chemical oxygen demand (COD) (COD), colourity, turbidity are all very high.As directly discharged without process, serious environmental pollution can be caused.
At present, the energy and environment safety problem become increasingly conspicuous, increases the active demand of people to recyclable organism fuel, this facilitates the further research and development of novel biological fuel-microbial oil.The research of microbial oil, can enrich traditional oil prodution industry technology, develops an important channel of suitability for industrialized production grease; Especially, when the contradiction of population growth, grease demand and natural resources critical shortage is becoming increasingly acute, the research of microbial oil has more practical significance.
Microbial oil, also known as Unicell Oils and Fats, is utilize carbohydrate, hydrocarbon polymer and common grease to be carbon source under certain condition by microorganisms such as yeast, mould, bacterium and algae, a large amount of greases produced in thalline.Sorbic acid wastewater treatment organic loading is high, containing carbohydrate, can provide nutritive substance for the metabolism of Lipid-producing microorganism, makes Lipid-producing microorganism run-up microbial oil.Importantly, Lipid-producing microorganism, while accumulation microbial oil, make use of the carbohydrate in sorbic acid wastewater treatment, therefore makes the COD of sorbic acid wastewater treatment reduce, reach the object of purifying treatment sorbic acid wastewater treatment.Utilize Lipid-producing microbiological treatment sorbic acid wastewater treatment, can microbial oil be obtained, the COD of waste water can be reduced again, reach the doulbe-sides' victory of economy and environment.
At present, cortex trichosporon is conventional Lipid-producing microorganism, can be used for the preparation of microbial oil.
Sorbic acid wastewater treatment colourity, turbidity are also very high.Microbial flocculant can be utilized to carry out pre-treatment to sorbic acid wastewater treatment, reduce colourity and the turbidity of sorbic acid wastewater treatment, be convenient to the Carbohydrate Accumulation microbial oil that follow-up Lipid-producing microorganism utilizes sorbic acid wastewater treatment, reduce the COD of sorbic acid wastewater treatment.At present, red city rhodococcus is usually used in the preparation of microbial flocculant.
Summary of the invention
The object of this invention is to provide a kind of Applied Biotechnology process sorbic acid wastewater treatment, while obtaining microbial oil, reduce the COD of sorbic acid wastewater treatment, realize the comprehensive utilization of sorbic acid wastewater treatment and the method utilizing cortex trichosporon process sorbic acid wastewater treatment of purification.
Step of the present invention is:
A, red city rhodococcus prepare microbial flocculant: be that the red city rhodococcus of CGMCC NO:4.1814 is linked in the aseptic fermention medium of 100 mL by the strain number be kept on inclined-plane, in described red city rhodococcus fermention medium, each amounts of components is respectively: sucrose, glucose, urea, yeast extract paste, CaCl
2, MgSO
47H
2o, NaCl; PH=8.0; 28 DEG C of shaking tables cultivate 3 days with 150 rpm, obtains microbial flocculant; After being cooled to room temperature, microbial flocculant being left in 4 DEG C of refrigerators and save backup;
The preparation of b, cortex trichosporon nutrient solution: be that CGMCC NO:2.0571 cortex trichosporon is linked in the aseptic seed culture medium of 100 mL by the strain number be kept on inclined-plane, in described cortex trichosporon seed culture medium, each amounts of components is respectively: glucose, yeast powder, (NH
4)
2sO
4, KH
2pO
4, MgSO
47H
2o, pH=6.5; 32 DEG C of shaking tables cultivate 1 day with 200 rpm, obtains cultured cortex trichosporon bacteria suspension, the bacteria suspension obtained is left in 4 DEG C of refrigerators and save backup;
C, microbial flocculant carry out pre-treatment to sorbic acid wastewater treatment: get the microbial flocculant that 2 mL step a obtain, put in 200 mL sorbic acid wastewater treatments, with coagulation agitator with 200 rpm rapid stirring 3 min, then 15 min are left standstill, double gauze filters disgorging, obtains the sorbic acid wastewater treatment that pre-treatment is good;
D, strain number be CGMCC NO:2.0571 cortex trichosporon to the process of sorbic acid wastewater treatment: get the cortex trichosporon bacteria suspension that 0.5 ~ 1 mL step b obtains, put in the good sorbic acid wastewater treatment of pre-treatment that 150 ~ 200 mL step c obtain, regulating the pH=7.0 of sorbic acid wastewater treatment with the NaOH that concentration is 0.01 mol/L, is with 100 rpm process 6 days on 22 DEG C of shaking tables in temperature.
The present invention utilizes red city rhodococcus to prepare microbial flocculant, carries out pre-treatment to sorbic acid wastewater treatment; And then utilize cortex trichosporon process sorbic acid wastewater treatment, while obtaining microbial oil, reduce the COD of sorbic acid wastewater treatment, realize comprehensive utilization and the purification of sorbic acid wastewater treatment.Its positively effect is:
1, utilize cortex trichosporon to process sorbic acid wastewater treatment, while obtaining microbial oil, reduce the COD of waste water, realize the doulbe-sides' victory of economy and environment, reach the object of energy-saving and emission-reduction;
2, utilize cortex trichosporon to process sorbic acid wastewater treatment, water treatment process is simple, easy to operate;
3, utilize cortex trichosporon and microbial flocculant to process sorbic acid wastewater treatment, the yield of microbial oil is higher, the obvious processing effect of waste water.Microbial oil yield remains on 15.55% ~ 17.26%; COD clearance remains on 79.29% ~ 84.41%.
Embodiment
Step of the present invention is:
A, red city rhodococcus prepare microbial flocculant
Be that the red city rhodococcus (Rhodococcus erythropolis) of CGMCC NO:4.1814 is linked in the aseptic fermention medium of 100 mL by the strain number be kept on inclined-plane, in described red city rhodococcus fermention medium, each amounts of components is respectively: sucrose 30.0 g/L; Glucose 5.0 g/L; Urea 12.0 g/L; Yeast extract paste 2.0 g/L; CaCl
21.8 g/L; MgSO
47H
2o 0.8 g/L; NaCl 0.3 g/L; PH=8.0; 28 DEG C of shaking tables cultivate 3 days with 150 rpm, obtains microbial flocculant; After being cooled to room temperature, microbial flocculant being left in 4 DEG C of refrigerators and save backup;
The preparation of b, cortex trichosporon nutrient solution
Be that CGMCC NO:2.0571 cortex trichosporon (Trichosporon cutaneum) is linked in the aseptic seed culture medium of 100 mL by the strain number be kept on inclined-plane, in described cortex trichosporon seed culture medium, each amounts of components is respectively: glucose 18.0 g/L; Yeast powder 1.0 g/L; (NH
4)
2sO
46.0 g/L; KH
2pO
40.8 g/L; MgSO
47H
2o 0.5 g/L; PH=6.5; 32 DEG C of shaking tables cultivate 1 day with 200 rpm, obtains cultured cortex trichosporon bacteria suspension, the bacteria suspension obtained is left in 4 DEG C of refrigerators and save backup;
C, microbial flocculant carry out pre-treatment to sorbic acid wastewater treatment
Get the microbial flocculant that 2 mL steps (1) obtain, put in 200 mL sorbic acid wastewater treatments, with coagulation agitator with 200 rpm rapid stirring 3 min, then leave standstill 15 min, double gauze filters disgorging, obtains the sorbic acid wastewater treatment that pre-treatment is good;
D, strain number are that CGMCC NO:2.0571 cortex trichosporon is to the process of sorbic acid wastewater treatment
Get the cortex trichosporon bacteria suspension that 0.5 mL step (2) obtains, put in the good sorbic acid wastewater treatment of pre-treatment that 200 mL steps (3) obtain, regulating the pH=7.0 of sorbic acid wastewater treatment with the NaOH that concentration is 0.01 mol/L, is with 100 rpm process 6 days on 22 DEG C of shaking tables in temperature; The clearance of measure and calculation sorbic acid wastewater treatment microbial oil yield and COD, realizes the preparation of microbial oil and the process of sorbic acid wastewater treatment.
Red city rhodococcus of the present invention and cortex trichosporon derive from China General Microbiological culture presevation administrative center, and strain number is respectively CGMCC NO:4.1814 and CGMCC NO:2.0571.
The microbial oil yield of sorbic acid wastewater treatment and COD treatment effect are in table 1.
Table 1 0.5 mL cortex trichosporon bacteria suspension is to the oil yield of 200 mL sorbic acid wastewater treatments and treatment effect
As can be seen from Table 1, through the process of cortex trichosporon to sorbic acid wastewater treatment, obtain microbial oil, and waste water COD is effectively degraded.
A kind of method utilizing cortex trichosporon process sorbic acid wastewater treatment of embodiment 2, it comprises the step (1) identical with embodiment 1, (2) and (3), and its difference step is:
(4) strain number is that CGMCC NO:2.0571 cortex trichosporon is to the process of sorbic acid wastewater treatment
Get the cortex trichosporon bacteria suspension that 0.7 mL step (2) obtains, put in the good sorbic acid wastewater treatment of pre-treatment that 170 mL steps (3) obtain, regulating the pH=7.0 of sorbic acid wastewater treatment with the NaOH that concentration is 0.01 mol/L, is with 100 rpm process 6 days on 22 DEG C of shaking tables in temperature; The clearance of measure and calculation sorbic acid wastewater treatment microbial oil yield and COD, realizes the preparation of microbial oil and the process of sorbic acid wastewater treatment.The microbial oil yield of sorbic acid wastewater treatment and COD treatment effect are in table 2.
Table 2 0.7 mL cortex trichosporon bacteria suspension is to the oil yield of 170 mL sorbic acid wastewater treatments and treatment effect
As can be seen from Table 2, through the process of cortex trichosporon to sorbic acid wastewater treatment, obtain microbial oil, and waste water COD is effectively degraded.
A kind of method utilizing cortex trichosporon process sorbic acid wastewater treatment of embodiment 3, it comprises the step (1) identical with embodiment 1, (2) and (3), and its difference step is:
(4) strain number is that CGMCC NO:2.0571 cortex trichosporon is to the process of sorbic acid wastewater treatment
Get the cortex trichosporon bacteria suspension that 1.0 mL steps (2) obtain, put in the good sorbic acid wastewater treatment of pre-treatment that 150 mL steps (3) obtain, regulating the pH=7.0 of sorbic acid wastewater treatment with the NaOH that concentration is 0.01 mol/L, is with 100 rpm process 6 days on 22 DEG C of shaking tables in temperature; The clearance of measure and calculation sorbic acid wastewater treatment microbial oil yield and COD, realizes the preparation of microbial oil and the process of sorbic acid wastewater treatment.The microbial oil yield of sorbic acid wastewater treatment and COD treatment effect are in table 3.
Table 3 1.0 mL cortex trichosporon bacteria suspension is to the oil yield of 150 mL sorbic acid wastewater treatments and treatment effect
As can be seen from Table 3, through the process of cortex trichosporon to sorbic acid wastewater treatment, obtain microbial oil, and waste water COD is effectively degraded.
Specific embodiments of the invention a pair content of the present invention has made detailed explanation, but is not limited to the present embodiment, any apparent change that those skilled in the art's enlightenment according to the present invention is done, and all belongs to the scope of rights protection of the present invention.
Claims (1)
1. utilize a method for cortex trichosporon process sorbic acid wastewater treatment, it is characterized in that: step is:
A, red city rhodococcus prepare microbial flocculant: be that the red city rhodococcus of CGMCC NO:4.1814 is linked in the aseptic fermention medium of 100 mL by the strain number be kept on inclined-plane, in described red city rhodococcus fermention medium, each amounts of components is respectively: sucrose, glucose, urea, yeast extract paste, CaCl
2, MgSO
47H
2o, NaCl; PH=8.0; 28 DEG C of shaking tables cultivate 3 days with 150 rpm, obtains microbial flocculant; After being cooled to room temperature, microbial flocculant being left in 4 DEG C of refrigerators and save backup;
The preparation of b, cortex trichosporon nutrient solution: be that CGMCC NO:2.0571 cortex trichosporon is linked in the aseptic seed culture medium of 100 mL by the strain number be kept on inclined-plane, in described cortex trichosporon seed culture medium, each amounts of components is respectively: glucose, yeast powder, (NH
4)
2sO
4, KH
2pO
4, MgSO
47H
2o, pH=6.5; 32 DEG C of shaking tables cultivate 1 day with 200 rpm, obtains cultured cortex trichosporon bacteria suspension, the bacteria suspension obtained is left in 4 DEG C of refrigerators and save backup;
C, microbial flocculant carry out pre-treatment to sorbic acid wastewater treatment: get the microbial flocculant that 2 mL step a obtain, put in 200 mL sorbic acid wastewater treatments, with coagulation agitator with 200 rpm rapid stirring 3 min, then 15 min are left standstill, double gauze filters disgorging, obtains the sorbic acid wastewater treatment that pre-treatment is good;
D, strain number be CGMCC NO:2.0571 cortex trichosporon to the process of sorbic acid wastewater treatment: get the cortex trichosporon bacteria suspension that 0.5 ~ 1 mL step b obtains, put in the good sorbic acid wastewater treatment of pre-treatment that 150 ~ 200 mL step c obtain, regulating the pH=7.0 of sorbic acid wastewater treatment with the NaOH that concentration is 0.01 mol/L, is with 100 rpm process 6 days on 22 DEG C of shaking tables in temperature.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111690587A (en) * | 2019-03-13 | 2020-09-22 | 华东理工大学 | Method for centrifugally screening grease yeast strains with high oil content and application thereof |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2259060A1 (en) * | 1974-01-29 | 1975-08-22 | British Petroleum Co | Biological purification of effluent - esp contg phenolics and detergents using Trichosporon cutaneum |
JPS59118089A (en) * | 1982-12-23 | 1984-07-07 | Agency Of Ind Science & Technol | Preparation of microbial flocculant using bacteria belonging to corynebacterium genus |
CN103121769A (en) * | 2013-02-05 | 2013-05-29 | 金能科技股份有限公司 | Sorbic acid wastewater treatment process |
CN103173497A (en) * | 2013-03-11 | 2013-06-26 | 宁波飞日水产实业有限公司 | Microbial flocculating agent preparation method |
-
2015
- 2015-07-03 CN CN201510383950.1A patent/CN105000673B/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2259060A1 (en) * | 1974-01-29 | 1975-08-22 | British Petroleum Co | Biological purification of effluent - esp contg phenolics and detergents using Trichosporon cutaneum |
JPS59118089A (en) * | 1982-12-23 | 1984-07-07 | Agency Of Ind Science & Technol | Preparation of microbial flocculant using bacteria belonging to corynebacterium genus |
CN103121769A (en) * | 2013-02-05 | 2013-05-29 | 金能科技股份有限公司 | Sorbic acid wastewater treatment process |
CN103173497A (en) * | 2013-03-11 | 2013-06-26 | 宁波飞日水产实业有限公司 | Microbial flocculating agent preparation method |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111690587A (en) * | 2019-03-13 | 2020-09-22 | 华东理工大学 | Method for centrifugally screening grease yeast strains with high oil content and application thereof |
CN111690587B (en) * | 2019-03-13 | 2022-10-25 | 上海凯赛生物技术股份有限公司 | Method for centrifugally screening grease yeast strains with high oil content and application thereof |
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