CN104988157A - OsTAL gene related to plant height and seed size and application of OsTAL gene - Google Patents

Abstract

The invention discloses an OsTAL gene related to plant height and seed size and application of the OsTAL gene. A protein is a protein which has an SEQ ID No:2 amino acid residue sequence in a sequence table, or a protein which has one or more amino acid residues replaced, lost or added in the SEQ ID No:2 amino acid residue sequence, has the same activity as the SEQ ID No:2 amino acid residue sequence and is derived from SEQ ID No:2. When the gene is expressed in a plant, the transgenic plant presents stronger growth vigor including the increase of plant height and the increase of hundred-grain weight in the mature period. According to the gene, the transgenic plant can be cultivated through the gene engineering method, agronomic traits such as plant type and hundred-grain weight are adjusted, and therefore the gene can be well applied to the improvement of crop varieties.

Description

The OsTAL gene that plant plant height is relevant with seed size and application thereof

Technical field

The invention belongs to plant genetic engineering field.Be specifically related to a kind of plant plant height OsTAL gene relevant with seed size and application thereof.

Background technology

Paddy rice is the most important food crop of China, is also one of most important food crop in the whole world.According to statistics, the whole world accounts for the population of 50% take rice as staple food.One of most important food crop of paddy rice Ye Shi China.According to statistics, 2008-2012 whole nation Monitoring of Paddy Rice Plant Area accounts for 27.2% of the food crop total area, and average annual production paddy total amount accounts for 35.7% (China Statistical Yearbook, 2013) of total grain output.

Plant type and seed size are the important determinatives of rice yield and rice quality.The sixties in 20th century, Mexican wheat, China and Filipine paddy rice improve output owing to reducing plant height, enhance lodging resistance, are called as " Green Revolution " (Monna L.DNAResearch, 2002,9 (1): 11-17; Spielmeyer, W.Proc Natl Acad SciU S A, 2002,99 (13): 9043-9048).Research shows, in this course, the raising of output, mainly round to utilize based on dwarf gene, is improved by plant type and carries out.The dwarf gene applied in paddy rice is sd1, and encode a GA ₂₀oxydase, take part in the synthesis of Plant hormones regulators,gibberellins.The dwarf gene utilized in present Rice Production and breeding still mainly sd1, the excessive use of same dwarf gene lies dormant by the single risk brought of heredity, and the trend of High-yield Rice Breeding is " asking high in short ", namely suitably plant height is improved, with increase biological yield provide paddy output (Yuan Longping. hybrid rice, 1996,2:1-3).On the other hand, while increase rice biomass production ability, also to improve " storage capacity " of paddy rice, namely increase paddy rice unit surface spike number, number of grain per ear and grain weight.Wherein, grain is heavily one of three elements of Yield And Yield Components, and seed is long, seed is wide and seed is thick is proportionate, grain heavy sometimes also with grain length and width and thick overall target weigh (before money. Science Press: paddy gene design and context, 2007,87).Therefore, the regulatory mechanism of Study On Rice plant height and seed size not only has theoretic meaning, also has important practical significance simultaneously.By the size of adjusting and controlling rice plant height height and seed, the output of paddy rice can be increased, and then ensure the grain security of China.

Summary of the invention

An object of the present invention is to provide a kind of plant plant height OsTAL gene relevant with seed size, the protein of this genes encoding.

Another object of the present invention is to provide a kind of method of cultivating the transgenic plant of plant height and seed size improvement.

The technical solution realizing the object of the invention is:

The invention provides the OsTAL gene that a kind of plant plant height is relevant with seed size, its nucleotide sequence is as shown in SEQID No:1.

Present invention also offers the protein of described OsTAL genes encoding, its aminoacid sequence is as shown in SEQ ID No:2.This protein, name is called OsTAL, derives from (the Oryza sativa L.cv.Wuyunjing Jiangsu authorization in 8,1999 of paddy rice Wuyunjing No.8, authorization numbering: Soviet Union's kind of No. 314th, careful word), be following (1) or the recombinant protein described in (2);

(1) protein be made up of the amino acid residue sequence of SEQ ID No:2;

(2) by the amino acid residue sequence in (1) through the replacement of one or several amino-acid residue and/or disappearance and/or interpolation and the protein that by (1) derived relevant to plant strain height, seed grain type or seed weight.

Above-mentioned SEQ ID No:2 is made up of 432 amino-acid residues.

The replacement of one or several amino-acid residue described and/or disappearance and/or interpolation refer to the replacement and/or disappearance and/or interpolation that are no more than 10 amino-acid residues.

The encoding gene of described albumen OsTAL also belongs to protection scope of the present invention.

The encoding gene of described albumen OsTAL is following 1) ~ 3) in arbitrary described DNA molecular

1) DNA molecular shown in SEQ ID No:1;

2) DNA sequence dna limited to SEQ ID No:1 under strict conditions hybridize and with plant species seed type or seed is wide or seed is thick or Seed weight is relevant DNA molecular;

3) DNA sequence dna limited with SEQ ID No:1 at least has 70%, at least have 75%, at least have 80%, at least have 85%, at least have 90%, at least have 95%, at least have 96%, at least have 97%, at least have 98% at least there is 99% homology and with plant plant height, seed grain type or seed is wide or seed is thick or seed heavy phase is closed DNA molecular.

Present invention also offers the plant expression vector containing said gene.

Recombinant vectors containing described encoding gene, recombinant bacterium, transgenic cell line or expression cassette are also the scope of protection of the invention.The promotor of described recombinant vectors is corn Uniquitin promotor UbiPro.

Described recombinant vectors obtains as follows:

1) cut pBI121 carrier with Sac I and EcoR I enzyme and obtain terminator Noster, be connected into same pCAMBIA1301 after Sac I and EcoR I enzyme cut, obtain carrier p1301NOS; With corn inbred line B73 genome (Schnable PS, Science.2009,326 (5956): 1112-1115) DNA is template, by the method amplification corn Uniquitin promotor of PCR, introduces Hind III and BamH I restriction enzyme site in PCR primer; Be connected in the p1301NOS carrier after Hind III and BamH I enzyme cut, obtain intermediate carrier p1301UN.

2), between the Kpn I encoding gene of OsTAL albumen being inserted intermediate carrier p1301UN and Sac I recognition site, recombinant vectors p1301UN-OsTAL is obtained.

Increase the primer pair in described OsTALl encoding gene encodes district; Described primer pair is as follows: upstream primer sequence is as shown in SEQID No:3, and downstream primer sequence is as shown in SEQ ID No:4.Kpn I and Sac I restriction enzyme site is introduced in PCR primer.

The present invention also provides a kind of method of cultivating the transgenic plant of plant height and seed size improvement.Be that described OsTALl encoding gene is imported object plant, cultivate the transgenic plant obtaining plant height and the improvement of grain type.

The improvement of described grain type is following 1)-4) at least one:

1) plant height of described transgenic plant is greater than described object plant;

2) seed grain of described transgenic plant is wider than described object plant;

3) seed grain of described transgenic plant is thick is greater than described object plant;

4) grain of the seed of described transgenic plant is great in described object plant.

Described grain is heavily 100-grain weight.

Described encoding gene is imported in described object plant by described recombinant vectors.

Described object plant is dicotyledons or monocotyledons, and described monocotyledons is preferably paddy rice, and the kind of described paddy rice is especially preferably Wuyunjing No.8.

This is compared with prior art, and its remarkable advantage is:

The OsTAL channel genes paddy rice Wuyunjing No.8 be cloned in japonica rice Wuyunjing No.8 is obtained the paddy rice of overexpression OsTAL gene, plant height, the seed of process LAN paddy rice are wide, seed is thick and seed 100-grain weight is all greater than described object plant.Confirm that this gene has the effect regulating Plant Height of Rice and seed size, increase the heavy output that effectively can increase crop of grain, thus can be advantageously applied to the improvement of crop varieties.OsTAL gene provided by the invention, the theoretical investigation not being only plant control plant height and seed size aspect provides important molecular genetics information, and also in raising crop yield, the genetic engineering breeding aspects such as the economical character of Crop Improvement provide the foundation.

Accompanying drawing explanation

Fig. 1 is the coding region with pcr amplification OsTAL gene, and Marker is DL 2000 (purchased from precious biotechnology (Dalian) company limited, production number: 3427A).

Fig. 2 is the plant expression vector figure of corn Ubiquitin promoters driven rice Os TAL gene (i.e. p1301UN-OsTAL fusion gene).

Fig. 3 is wild-type Wuyunjing No.8 and the mRNA transcript analysis turning OsTAL in p1301UN-OsTAL fusion gene plant.In wild-type Wuyunjing No.8, the mRNA transcript of OsTAL gene is very low, and the mRNA transcript degree turning OsTAL in p1301UN-OsTAL fusion gene plant very high (* * represents P<0.01 level difference).

Fig. 4 is wild-type Wuyunjing No.8 and the statistics (* * represents P<0.01 level difference) turning p1301UN-OsTAL fusion gene plant plant height.

Fig. 5 is wild-type Wuyunjing No.8 and turns the long statistics of p1301UN-OsTAL fusion gene plant seed.

Fig. 6 is that (* represents P<0.05 level difference to wild-type Wuyunjing No.8 with turning the wide statistics of p1301UN-OsTAL fusion gene plant seed; * represents P<0.01 level difference).

Fig. 7 is wild-type Wuyunjing No.8 and turns the thick statistics (* * represents P<0.01 level difference) of p1301UN-OsTAL fusion gene plant seed.

Fig. 8 is wild-type Wuyunjing No.8 and the statistics (* * represents P<0.01 level difference) turning p1301UN-OsTAL fusion gene plant seed 100-grain weight.

Fig. 9 is wild-type Wuyunjing No.8 and turns p1301UN-OsTAL fusion gene plant forms.

Figure 10 is wild-type Wuyunjing No.8 and the Grain Morphology turning p1301UN-OsTAL fusion gene.

Embodiment

Below in conjunction with accompanying drawing, the present invention is described in further detail, but does not limit the present invention.

Experimental technique in following embodiment, comprises DNA extraction, RNA extraction, the preparation of cDNA, enzyme is cut, the structure of cloning and expressing carrier, connection, bacterial strain transform and screening etc., if no special instructions, be the ordinary method in this area.

In following embodiment, the various experiment materials, reagent, enzyme, carrier etc. that use, all obtain by commercial sources.

Embodiment 1

The clone of OsTAL gene:

The Trizol extraction method of this area routine is adopted to extract the total mRNA of rice leaf, DNase I is (purchased from precious biotechnology (Dalian) company limited, production number: 2270A) process after, by M-MLV ThermoScript II (purchased from precious biotechnology (Dalian) company limited, production number: 2641Q) reverse transcription synthesis cDNA, reaction system and reaction conditions are with reference to related kit explanation.Utilize this cDNA for pcr template, the encoding sequence SEQ ID No:1 of amplification OsTAL gene, the Auele Specific Primer used that increases is as follows:

Upstream primer (SEQ ID No:3): AAA gGTACCaTGACCGGCGCCGTCTCCA

Underlined sequences is Kpn I site;

Downstream primer (SEQ ID No:4): AAA gAGCTCcTACAGGCTCACGGTCTTCTTGA

Underlined sequences is Spe I site.

PCR reaction system: 10 х Taq Buffer 5 μ L, 10mM dNTP Mix 2 μ L, upstream primer (SEQ ID No:3) (10 μm of ol/L) 2 μ L, downstream primer (SEQ ID No:4) (10 μm of ol/L) 2 μ L, cDNA template 2 μ L, high-fidelity Taq enzyme (5U/ μ L) 1 μ L and ddH ₂o 36 μ L.

PCR response procedures: enter PCR circulation after 94 DEG C of denaturation 3min, loop parameter is that DEG C 30s renaturation → 72,94 DEG C of 30s sex change → 55 DEG C 2min extends, and carries out 35 circulations, finally at 72 DEG C of downward-extension 10min.

The PCR primer increased is separated through the agarose gel electrophoresis of 1%, result as shown in Figure 1, as can be seen from the figure, obtain the band of the about 1.3Kb of molecular weight, test kit is reclaimed (purchased from precious biotechnology (Dalian) company limited with DNA gel, production number: 7345) reclaim this fragment, obtains and reclaims product.By PCR primer and pZeroBack/blunt carrier (purchased from TIANGEN Biotech (Beijing) Co., Ltd., production number: VT204-01) at 16 DEG C, connect 30min, 42 DEG C of thermal shock 60s, transformation of E. coli DH5 α, conversion product is growing containing on the LB plate culture medium of penbritin, select positive colony, extract plasmid and check order (entrusting Shenzhen Hua Da gene to complete), the Nucleotide containing sequence table SEQ ID No:1 in this plasmid.By this plasmid called after p-OsTAL.

Embodiment 2

The acquisition of OsTAL overexpression recombinant vectors:

The construction process of p1301UN carrier is:

Get 0.1g Leaves of Maize Seedlings, be placed in liquid nitrogen and grind, add 800 μ L Extraction buffers (0.1M Tris-HclpH8.0,50mM EDTA, 0.5M NaCl, 1%SDS and 1% beta-mercaptoethanol), repeat concussion and suspend; 65 DEG C of water-bath 40min, every 10min mixing is once; Then add the 5M potassium acetate of 250 μ L precoolings, put upside down mixing, ice bath 5min; Add equal amounts of phenolic/chloroform, mixing extracting; The centrifugal 5min of 12000rpm; Draw supernatant in another centrifuge tube, add the Virahol of 0.6 times of volume, room temperature is placed 60min and is precipitated DNA; 4 DEG C of centrifugal 15min of 12000rpm, abandon supernatant; Precipitate with 70% ethanol wash once; After drying, add 20 μ L ddH ₂o dissolves, and obtains corn gene group DNA.With this DNA for template, (upstream primer sequence is: AAA to add upstream and downstream primer aAGCTTcTGCAGTGCAGCGTGACCCGGTCGTG, downstream primer sequence is: AAA gGATCCtGCAGAAGTAACACCAAACAACAGGG) carry out pcr amplification, in primer, introduce Hind III and BamH I restriction enzyme site (dashed part sequence) respectively.

PCR response procedures: enter PCR circulation after 94 DEG C of denaturation 3min, loop parameter is that DEG C 30s renaturation → 72,94 DEG C of 30s sex change → 59 DEG C 2min extends, and carries out 35 circulations, finally at 72 DEG C of downward-extension 10min.The PCR primer increased is separated through the agarose gel electrophoresis of 1%, and (purchased from precious biotechnology (Dalian) company limited, production number: 7345) reclaim this fragment, obtains and reclaim product to reclaim test kit with DNA gel.By PCR primer and pZeroBack/blunt carrier (purchased from TIANGEN Biotech (Beijing) Co., Ltd., production number: VT204-01) at 16 DEG C, connect 30min, 42 DEG C of thermal shock 60s, transformation of E. coli DH5 α, conversion product is growing containing on the LB plate culture medium of penbritin, select positive colony, extract plasmid and check order (entrusting Shenzhen Hua Da gene to complete), Nucleotide containing corn Ubiquitin promotor in this plasmid, by this plasmid called after p-UbiPro.

Cut pBI121 carrier with Sac I and EcoR I enzyme and obtain terminator Noster, be connected into same pCAMBIA1301 after Sac I and EcoR I enzyme cut, obtain carrier p1301NOS; Ubiquitin promotor cut from p-UbiPro with Hind III and BamH I double digestion, the p1301NOS after cutting through same enzyme enzyme connects again, and obtains intermediate carrier p1301UN.

The acquisition of recombinant vectors p1301UN-OsTAL:

With the p-OsTAL plasmid obtained in Kpn I and Sac I double digestion embodiment 1, the enzyme system of cutting is: 10 х enzymes cut Buffer 5 μ L, p-OsTAL plasmid 15 μ L, Kpn I (5U/ μ L) and Sac I (5U/ μ L) each 1 μ L, ddH ₂o 28 μ L.37 DEG C of enzymes cut 12 hours.Digestion products is separated through the agarose gel electrophoresis of 1%, test kit is reclaimed (purchased from precious biotechnology (Dalian) company limited with DNA gel, production number: the OsTAL gene coding region DNA fragmentation 7345) reclaiming about 1.3Kb, is dissolved in 30 μ L ddH ₂for subsequent use in O.

Adopt the p1301UN that substep enzyme cutting method acquisition Kpn I and Sac I enzyme are cut: first, with Kpn I single endonuclease digestion intermediate carrier p1301UN, the enzyme system of cutting is: 10 х enzymes cut Buffer 3 μ L, p1301UN plasmid 15 μ L, Kpn I (5U/ μ L) 1 μ L, ddH ₂o 11 μ L; 37 DEG C of enzymes cut 4 hours; Then the 3M CH3COONa (pH 5.2) of 1/10 volume is added, mixing; Add the precooling dehydrated alcohol of 2.5 times of volumes, mixing, place 60min for-20 DEG C; 12000rpm, 4 DEG C of centrifugal 10min, reclaim precipitation; Then use the pre-cooled ethanol washing and precipitating of 1ml 70%, 12000rpm, 4 DEG C of centrifugal 10min, removing supernatant, drying at room temperature precipitates; Precipitate with 20 μ L ddH ₂o dissolves; Cut by recovery product continuation Sac I enzyme, the enzyme system of cutting is: 10 х enzymes cut Buffer 3 μ L, p1301UN plasmid 20 μ L, Kpn I (5U/ μ L) 1 μ L, ddH ₂o 6 μ L; 37 DEG C of enzymes cut 4 hours.Digestion products is separated through the agarose gel electrophoresis of 1%, and (purchased from precious biotechnology (Dalian) company limited, production number: 7345) reclaim linearizing p1301UN carrier segments, is dissolved in 30 μ L ddH to reclaim test kit with DNA gel ₂for subsequent use in O.

The OsTAL gene fragment of above-mentioned recovery and p1301UN carrier segments are carried out ligation, and linked system is: OsTAL gene fragment 6 μ L, p1301UN carrier segments 2 μ L, 10 х connect Buffer 1 μ L, T ₄ligase enzyme 1 μ L; After mixing, 16 DEG C connect 12 hours, then product conversion bacillus coli DH 5 alpha will be connected, conversion product is growing containing on the LB plate culture medium of kantlex, select positive colony, extract plasmid and qualification of checking order (entrusting Shenzhen Hua Da gene to complete), the structure of result corn Ubiquitin promotor, OsTAL gene and Noster terminator is correct, by this plasmid called after p1301UN-OsTAL.The physical map of this recombinant vectors as shown in Figure 2.

Embodiment 3

Acquisition containing p1301UN-OsTAL recombinant vectors transgenic paddy rice

P1301UN-OsTAL recombinant plasmid transformed Agrobacterium A.Tumefaciens EHA105, the LB substratum containing kantlex chooses mono-clonal, and identifies positive colony with PCR.

This is imported the Agrobacterium-mediated Transformation paddy rice Wuyunjing No.8 callus of p1301UN-OsTAL recombinant plasmid, through callus of induce, infect, callus that Dual culture, screening have resistance, break up, take root, transplant, finally obtain transfer-gen plant.Main method (the Agrobacterium-mediatedtransformation of rice using immature embryos or calli induced from mature seed with reference to people such as Hiei of agriculture bacillus mediated rice transformation system, NatureProtocols, 2008 (3): 824-834) basis is carried out.Central genetic step of converting is as follows:

1, the seed of full Wuyunjing No.8 is selected, the seed of maturation is shelled, with the alcohol disinfecting 30s-1min of 75%, then clorox sterilizing (clorox/distilled water=1:2 is added, abundant mixing), add 1-2 again and drip Tween-20, leave standstill 35-40min, period shakes once gently every 10min.Then rinsed with sterile water 3-5 time is used, air-dry, be inoculated in N ₆d ₂on substratum, 28 DEG C of light culture 10-15 days.The callus derived is cut, in N ₆d ₂subculture 3-5 days on substratum.

2, the single colony inoculation of Agrobacterium of picking importing p1301UN-OsTAL recombinant plasmid is in 3ml containing in the LB liquid nutrient medium of 50mg/l kantlex, at 28 DEG C of shaking culture 16h, then inoculates in LB liquid nutrient medium by 1/100 (v/v) inoculum size.When being cultured to logarithmic phase, collected by centrifugation Agrobacterium is also resuspended in appropriate AAM (Syringylethanone containing 100-400 μm of ol/l) liquid nutrient medium, is soaked into by the callus cultivating Wuyunjing No.8 in this Agrobacterium bacterium liquid.After infecting 15-20min, callus is placed on aseptic filter paper and sucks too much bacterium liquid, then callus is proceeded to N ₆d ₂c substratum carries out Dual culture under 28 DEG C of dark conditions.Callus, after 3 days, is proceeded to the Selective agar medium CCD containing 25mg/L Totomycin and 600mg/L cephamycin by Dual culture ₂s ₁on carry out first round screening and culturing; After 10 days, the fresh kanamycin-resistant callus tissue grown is proceeded to again the Selective agar medium CCD containing 50mg/L Totomycin and 300mg/L cephamycin ₂s ₂upper continuation screening.

3, callus is after the screening of 2 generations, and the vigorous fresh resistant calli of growth selection is transferred on the CCA substratum containing 50mg/L Totomycin and 300mg/L cephamycin and carry out pre-differentiation culture under 28 DEG C of dark conditions.After 10 days, then break up under the resistant calli of pre-differentiation culture being transferred to dark in 16h light/8h on the division culture medium MSR containing 50mg/L Totomycin and 300mg/L cephamycin, 28 DEG C of conditions.

4, the seedling of regeneration strong plantlets and rootage on the 1/2MS substratum containing 12.5mg/l Totomycin and 150mg/l cephamycin, finally transfers to field cultivation.The cultivation management of transgenic paddy rice carries out according to a conventional method.

The substratum that table 1 rice tissue uses in cultivating and transforming and composition thereof

Above-mentioned plant expression vector pCAMBIA1301, pBI121, bacillus coli DH 5 alpha and Agrobacterium A.TumefaciensEHA105 are the experiment material that those skilled in the art commonly use.PCR involved in above-mentioned molecule manipulation process, enzyme are cut, connect, Bacillus coli cells transforms and the conversion of Agrobacterium is the routine operation of this area.

Embodiment 4

Molecular Identification containing p1301UN-OsTAL transgenic plant and phenotype analytical

Obtain 14 independently T altogether ₀generation turn p1301UN-OsTAL paddy rice, by these plant together with unconverted acceptor Wuyunjing No.8 at field planting.T is extracted by Trizol method ₀for the total serum IgE in the transfer-gen plant of overexpression OsTAL gene and unconverted acceptor Wuyunjing No.8 blade, reverse transcription obtains the first chain cDNA, utilizing real-time fluorescence quantitative PCR, is that primer is analyzed the transcriptional level of OsTAL in the transfer-gen plant of 3 wherein at random with gene specific sequence.Reverse Transcription for real-time fluorescence quantitative PCR is PrimeScript ^tMrT Master Mix (purchased from precious biotechnology (Dalian) company limited, production number: RR036A), reaction system and reaction conditions are with reference to specification sheets.Reagent for Real time PCR is premix Ex Taq ^tMiI (purchased from precious biotechnology (Dalian) company limited, production number: RR820A).Instrument is Life Technologies company of U.S. real-time fluorescence quantitative PCR instrument ABIVii A7.Reaction system is with reference to specification sheets.OsTAL gene specific upstream primer sequence is: 5'-AGATACGAGGCTGTGATTGA-3'; Downstream primer sequence is: 5'-TCTTGGCACCTTTCTTGAC-3'.With Actin gene crops internal reference, its upstream primer sequence is: 5'-GATGACCCAGATCATGTTTG-3'; Its downstream primer sequence is: 5'-GGGCGATGTAGGAAAGC-3'.PCR program is: denaturation 3min, then enters PCR circulation, and loop parameter is that DEG C 15s renaturation → 72,94 DEG C of 15s sex change → 55 DEG C 40s extends, and carries out 40 circulations.

Result as shown in Figure 3, compared with unconverted contrast Wuyunjing No.8, when Actin gene is as internal reference, in 7 transfer-gen plants, the transcriptional level of OsTAL all has remarkable rise, illustrate that target gene OsTAL successfully imports in acceptor Wuyunjing No.8, and played effect.Gather in the crops this 3 T ₀for the seed of transfer-gen plant, plantation obtains T ₁for plant, results T ₂for seed, then plant T ₂for transfer-gen plant.

Fig. 4 ~ Fig. 8 respectively illustrates T ₂plant plant height, seed are long, seed is wide, seed is thick and seed 100-grain weight statistical graph, investigate 10-20 strain respectively, results averaged.Result shows, 112% (120.5cm), 110% (118.2cm) and 106% (113.7cm) of to be the plant height of 107.7cm, OsTAL overexpression transgenic line (strain is numbered: ZY14895, ZY14906, ZY14908) be the respectively contrast of the plant height of unconverted contrast Wuyunjing No.8; The grain length of unconverted contrast Wuyunjing No.8 is 7.2355mm, and the grain length of overexpression transgenic line (ZY14895, ZY14906, ZY14908) is 101% (7.3423mm), 103% (7.4400mm) and 103% (7.4775mm) of contrast respectively; The grain of unconverted contrast Wuyunjing No.8 is wide is 3.1840mm, and the grain of overexpression transgenic line (ZY14895, ZY14906, ZY14908) is wide is 104% (3.3270mm), 107% (3.4220mm) and 110% (3.4870mm) that contrast respectively; The grain of unconverted contrast Wuyunjing No.8 is thick is 2.3035mm, and the grain of overexpression transgenic line (ZY14895, ZY14906, ZY14908) is thick is 105% (2.4250mm), 108% (2.4790mm) and 107% (2.4665mm) that contrast respectively; The 100-grain weight of unconverted contrast Wuyunjing No.8 is 2.6781g, and the 100-grain weight of overexpression transgenic line (ZY14895, ZY14906, ZY14908) is 104% (2.7720g), 106% (2.8460g) and 106% (2.8350g) of contrast respectively.

Plant and seed phenotype are distinguished as shown in Figure 9 and Figure 10, compared with unconverted contrast Wuyunjing No.8, and T ₂plant Height of Rice for overexpression OsTAL gene increases, and seed becomes large (strain numbering: ZY14908).

Claims (9)

1. the OsTAL gene that plant plant height is relevant with seed size, is characterized in that, its nucleotide sequence is as shown in SEQID No:1.

2. a protein for the OsTAL genes encoding that plant plant height described in claim 1 is relevant with seed size, is characterized in that, its aminoacid sequence is as shown in SEQ ID No:2.

3. an expression vector, is characterized in that, containing nucleotide sequence according to claim 1.

4. a recombinant bacterial strain, is characterized in that, containing expression vector according to claim 3.

5. cultivate a method for the transgenic plant of plant height and seed size improvement, it is characterized in that, comprising the channel genes object plant with having the nucleotide sequence shown in SEQ ID No:1, cultivate the transgenic plant obtaining plant height and the improvement of grain type.

6. the method for transgenic plant of cultivation plant height according to claim 5 and seed size improvement, is characterized in that, described grain type improvement is following 1)-4) at least one:

1) plant height of described transgenic plant is greater than described object plant;

2) seed grain of described transgenic plant is wider than described object plant;

3) seed grain of described transgenic plant is thick is greater than described object plant;

4) grain of the seed of described transgenic plant is great in described object plant, and described grain is heavily 100-grain weight.

7. the method for the transgenic plant of cultivation plant height according to claim 6 and seed size improvement, it is characterized in that, described object plant is dicotyledons or monocotyledons,

8. the method for the transgenic plant of cultivation plant height according to claim 7 and seed size improvement, it is characterized in that, described monocotyledons is paddy rice,

9. the method for the transgenic plant of cultivation plant height according to claim 8 and seed size improvement, it is characterized in that, the kind of described paddy rice is Wuyunjing No.8.