CN104988061A - SOD enzyme activity integrating light reaction experimental instrument and applications thereof - Google Patents
SOD enzyme activity integrating light reaction experimental instrument and applications thereof Download PDFInfo
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- CN104988061A CN104988061A CN201510126121.5A CN201510126121A CN104988061A CN 104988061 A CN104988061 A CN 104988061A CN 201510126121 A CN201510126121 A CN 201510126121A CN 104988061 A CN104988061 A CN 104988061A
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Abstract
The present invention provides a SOD enzyme activity integrating light reaction experimental instrument and applications thereof, wherein a 80C51 microcontroller is adopted as a microprocessor, an illuminance sensor is used to control light intensity so as to achieve real-time precise regulation, a time control circuit is provided to control the light reaction time, a temperature control instrument is used to adjust and control the ambient temperature of the light reaction, and the ambient temperature change is subjected to thermal-cold adjusting by using a magnetic disk type heater and a semiconductor air conditioner. According to the present invention, the enzyme activity experiment is integrated as more as possible so as to improve the experiment precision and the experiment efficiency; and the results are subjected to multiple experiment measurements, the overall performance is good, the technology is advanced, the effect is good, the regulation is easy, the precision and the repeatability are greatly improved, and the originality and the promotional value are provided.
Description
Technical field
The invention belongs to Biochemistry Experiment instrument field, particularly relate to a kind of integrated SOD enzyme activity photoresponse experiment instrument and application thereof.
Background technology
After nineteen sixty-eight finds superoxide-dismutase (Superoxide Dismutase, SOD), cause the great attention of scientific circles at once, over nearly 40 years, the progress of this respect is very fast, and its Application Areas is widened day by day, and SOD there has also been product.In the twentieth century later stage eighties, China also form focus about the Study and appliance of SOD, nowadays cosmetics additive, beverage and medical in show special-effect.
Superoxide radical is the common intermediate product of some biochemical reactions of biomass cells.Free radical is the molecule with paired valence electron own, atom, atomic group or ion, and chemical property is very active, is the one of active oxygen.If when lacking the enzyme of scavenging free radicals in cell, body will be subject to various damage.The superoxide radical in biomass cells is removed, Hydrogen Peroxide and O by disproportionation reaction
2.Hydrogen peroxide generates H by catalase (CAT) catalysis
2o and O
2, thus reduce the organic murder by poisoning of radical pair.
Because superoxide radical is unstable radical, the life-span is extremely short, measures SOD activity and is generally indirect method.And utilize various color reaction to measure the vigor of SOD.Riboflavin can produce superoxide radical negative ion under aerobic conditions, after adding NBT, under illumination condition, react with superoxide radical and generate single first moon for (yellow), then reduction generates and replaces by the diformazan moon, and it is a kind of blue material, under 560nm wavelength, have maximum absorption.When adding SOD, superoxide radical can be made to be combined with H and to generate H
2o
2and O
2, thus inhibit the carrying out of NBT photoreduction, the blue diformazan moon is slowed down for formation speed.By adding the SOD enzyme liquid of different amount in reaction solution, each liquid optical density value under measuring 560nm wavelength after illumination certain hour, suppress NBT photoreduction relative percentage and enzymic activity proportional within the specific limits.NBT photoreduction relative percentage is suppressed to calculate enzymic activity according to SOD.Find out enzyme amount when SOD suppresses NBT photoreduction relative percentage to be 50% as an enzyme activity unit (U).
SOD enzyme activity detects, and in life science, in chemistry and biophysical studies, is an extremely important and very general experiment, mainly for detection of the index such as resistance enzymic activity, content of organism.But photoreduction method is in actual experiment process, owing to there is no specific equipment, exist random in intensity control, temperature control etc., there is comparatively big error in lack of standardization, not rigorous making, so that can not calculate the active size of SOD enzyme exactly in experimentation.
Summary of the invention
The object of the present invention is to provide a kind of integrated SOD enzyme activity photoresponse experiment instrument and application thereof, be intended to solve existing SOD enzyme activity and detect shortage specific equipment, the inaccurate problem of active size detection of SOD enzyme.
The present invention is achieved in that a kind of integrated SOD enzyme activity photoresponse experiment instrument, comprises illumination lamp, light-operated timing system and temperature controlling system; Wherein,
Described light-operated timing system comprises: illuminance sensor, for gathering the illumination intensity value of described illumination lamp irradiated region; Luminosity control module, for regulating and controlling the intensity of illumination of illumination lamp; Button time block, for carrying out timing to the light application time of described illumination lamp and the acquisition time of illuminance sensor illumination; Alarm module, for giving the alarm after described button time block timing terminates; LCD display module, for showing described intensity of illumination and light application time; And micro-chip, for illuminance sensor is transmitted illumination intensity value, button time block timing show in LCD display module, and when reaching timing control alarm module give the alarm; Described micro-chip is connected with illuminance sensor, luminosity control module, button time block, alarm module and LCD display module respectively;
Described temperature controlling system comprises: temperature sensor, for gathering the temperature value of illumination lamp irradiated region; Temperature raising device, heats for giving illumination lamp irradiated region; Refrigeration equipment, lowers the temperature for giving illumination lamp irradiated region; And temperature controller;
Described temperature controller comprises: display control module, for controlling temperature raising device and refrigeration equipment work by keying input mode, and the temperature variation of the temperature value of illumination lamp irradiated region, temperature raising device and refrigeration equipment is shown; And data processing module, for the keying instruction control temperature sensor according to display control module, temperature raising device and refrigeration equipment work, and show on display control module after the signal received is carried out data processing;
Described temperature controller is connected with temperature sensor, temperature raising device, refrigeration equipment respectively;
Described illumination lamp, temperature controller are connected with micro-chip respectively.
Preferably, this tester also comprises shell, and described shell comprises housing and lid; Install micro-chip, refrigeration equipment, temperature raising device and data processing module in described housing, described surface of shell installs button time block, alarm module, LCD display module and display control module; Be provided with reactive tank above described housing, install illumination lamp, temperature sensor and illuminance sensor in described reactive tank, the notch of described reactive tank installs lid.
Preferably, be provided with bracket in described reactive tank, described bracket is made up of the plate face of mesh and pillar stiffener, is fixed on reaction tank bottom, top support plate face bottom described pillar stiffener; Wherein, described illumination lamp, temperature sensor and illuminance sensor are positioned at below described plate face.
Preferably, described micro-chip is 80C51 micro-chip.
Preferably, described alarm module is hummer, and described LCD display module is LCD1602.
Preferably, described temperature raising device is magnetic sheet calorifier, and described refrigeration equipment is semiconductor air conditioner.
Invention further provides the application of above-mentioned integrated SOD enzyme activity photoresponse experiment instrument in SOD enzyme activity context of detection.
The present invention overcomes the deficiencies in the prior art, a kind of integrated SOD enzyme activity photoresponse experiment instrument and application thereof are provided, adopt 80C51 micro-chip as microprocessor, control the real-time accuracy controlling of light intensity by illuminance sensor, time control circuit is provided, controls optical response time; In addition, adopt temperature controller, the envrionment temperature of regulable control photoresponse, variation of ambient temperature employing magnetic sheet calorifier, semiconductor air conditioner carry out hot cool tone joint.The present invention, by by integrated as much as possible for Enzyme assay experiment, improves experimental precision and conventional efficient.Result is measured through many experiments, and overall performance is good, and advanced technology is respond well, and manipulation is convenient, and precision and repeatability improve greatly, have originality and promotional value.
Accompanying drawing explanation
Fig. 1 is the structural representation of light-operated timing system in the present invention's integrated SOD enzyme activity photoresponse experiment instrument;
Fig. 2 is the structural representation of temperature controlling system in the present invention's integrated SOD enzyme activity photoresponse experiment instrument;
Fig. 3 is the structural representation of the present invention's integrated SOD enzyme activity photoresponse experiment instrument housing;
Fig. 4 is the using state figure of LCD1602 display panel in the present invention's integrated SOD enzyme activity photoresponse experiment instrument;
Fig. 5 is that the LCD1602 of the present invention's integrated SOD enzyme activity photoresponse experiment instrument shows state graph.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The invention provides a kind of integrated SOD enzyme activity photoresponse experiment instrument, comprise illumination lamp, light-operated timing system and temperature controlling system, shell; Wherein, described light-operated timing system, as shown in Figure 1, comprising: illuminance sensor 10, for gathering the illumination intensity value of described illumination lamp irradiated region; Luminosity control module 11, for regulating and controlling the intensity of illumination of illumination lamp; Button time block 12, for carrying out timing to the light application time of described illumination lamp and the acquisition time of illuminance sensor illumination; Alarm module 13, for giving the alarm after described button time block timing terminates; LCD display module 14, for showing described intensity of illumination and light application time; And micro-chip 15, the timing for regulating illumination intensity, the illumination intensity value transmitted by illuminance sensor, button time block shows in LCD display module, and control alarm module gives the alarm when reaching timing; Described micro-chip 15 is connected with illuminance sensor 10, luminosity control module 11, button time block 12, alarm module 13 and LCD display module 14 respectively.
Described temperature controlling system, as shown in Figure 2, comprising: temperature sensor 20, for gathering the temperature value of illumination lamp irradiated region; Temperature raising device 21, heats for giving illumination lamp irradiated region; Refrigeration equipment 22, lowers the temperature for giving illumination lamp irradiated region; And temperature controller 23; Described temperature controller 23 comprises: display control module 230, for being worked by keying input mode control temperature raising device 21 and refrigeration equipment 22, and the temperature variation of the temperature value of illumination lamp irradiated region, temperature raising device 21 and refrigeration equipment 22 is shown; And data processing module 231, work for the keying instruction control temperature sensor 20 according to display control module 230, temperature raising device 21 and refrigeration equipment 22, and show on display control module 230 after the signal received being carried out data processing; Described temperature controller 23 is connected with temperature sensor 20, temperature raising device 21, refrigeration equipment 22 respectively.
Described illumination lamp, temperature controller 23 are connected with micro-chip 15 respectively.
In actual applications, described micro-chip is 80C51 micro-chip.The regulative mode of luminosity control module 11 pairs of illuminance is manual/auto two kinds of modes, and wherein manual regulation realizes by adjusting knob, automatically regulates and is realized by function software.Described alarm module 13 is hummer, and described LCD display module 14 is LCD1602.
Described temperature raising device 21 is magnetic sheet calorifier, described refrigeration equipment 22 is semiconductor air conditioner, described temperature controller 23, namely temperature controller is that society of Japan Chemical strain society produces, the REX-C400 type Digital Displayed Temperature Controller that available from the glad trade Co., Ltd of Xiamen Photar.
Because temperature controller includes microprocessor, the circuit such as its detection, information acquisition, display, control can independent component composition, to avoid and the influencing each other, to reduce failure rate of other circuit.
Described shell, as shown in Figure 3, comprises housing 30 and lid 31; Micro-chip 15, refrigeration equipment 21, temperature raising device 22 and data processing module 231 are installed, described housing 30 surface mounting luminosity control module 11, button time block 12, alarm module 13, LCD display module 14 and display control module 230 in described housing 30; Be provided with reactive tank 32 above described housing 30, install illumination lamp, temperature sensor 20 and illuminance sensor 10 in described reactive tank 32, the notch of described reactive tank 32 installs lid 31.For the ease of placing testing sample and insulation, minimizing interference, more specifically, be provided with bracket 33 in described reactive tank 32, described bracket 33 is made up of the plate face of mesh and pillar stiffener, is fixed on bottom reactive tank 32, top support plate face bottom described pillar stiffener; Wherein, described illumination lamp, temperature sensor 20 and illuminance sensor 10 are positioned at below described plate face.In embodiments of the present invention, illumination lamp is two rows and is arranged on bracket 33 both sides respectively, and two row's illumination lamps share a circuit, to make to control simultaneously.
In actual application of the present invention, the specific works parameter of the present invention's integrated SOD enzyme activity photoresponse experiment instrument is as follows:
Temperature control scope: 10 DEG C ~ 50 DEG C; Temperature resolution: 0.1 DEG C; Temperature fluctuation: ± 0.5 DEG C
Temperature regulation mechanism: manually/setting/automatically
Temperature rise rate: be not less than 0.5 DEG C/min; Rate of temperature fall: be not less than 0.2 DEG C/min
Temperature sampling speed: 0.1s/ time
Illuminance: warm white 0-6000Lx; Illuminance fluctuates: 2% of ± set(ting)value
Illuminance regulative mode: manual/auto/setting
Illuminance sampling rate: 0.1s/ time
Illuminance internal regulation: current setting/voltage-regulation
Time sets: 0-59min59s
Timing mode: setting
Rated output: 1KW (maximum value)
Working power: 220V ~ 240V/50Hz ~ 60Hz
The actual operation instruction of the present invention's integrated SOD enzyme activity photoresponse experiment instrument is as follows:
(1) key part (comprising timing, illumination, temperature setting etc.), as shown in Figure 4, wherein:
1. key (reserving) is expanded;
2. reset key, data initial value is " 59:59 ", and experiment each time terminates when testing next time again, all will by " reset key " initialize, otherwise the time cannot set;
3. OK button, presses this key, and both can set " second ", and setting terminates to press " determination " key, can set " minute ";
4. increase key, press this key once, current controlled quatity (comprising the amount that time, illumination, temperature etc. regulate) numerical value increases by 1;
5. reduce key, press this key once, current controlled quatity numerical value reduces 1.
(2) LCD1602 display section, as shown in Figure 5, wherein: the first row " Time " displaying time, the second row " Light " display illuminance now.
(3) Illumination adjusting
The present invention adopts two lamp plate illumination, and each lamp plate has an adjustable knob to control, and in order to reduce the error in regulate process, adds a scale card, is convenient to regulate.
(4) illumination collection
Illuminance sensor uses BH1750 ambient light sensor, and the digital quantity that this module exports is directly inputted in micro-chip, then display light illumination constantly.
(5) alarm portion
At the end of setting-up time countdown, hummer can send audible warning.
Compared to the shortcoming and defect of prior art, the present invention has following beneficial effect: specification of the present invention is rigorous, easy to operate, and design concept is advanced, and successful, has higher integrated level; Showing through using, reach design requirements, and design concept is more advanced, rational in infrastructure, there is wide popularizing application prospect.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. an integrated SOD enzyme activity photoresponse experiment instrument, is characterized in that, comprises illumination lamp, light-operated timing system and temperature controlling system; Wherein,
Described light-operated timing system comprises: illuminance sensor, for gathering the illumination intensity value of described illumination lamp irradiated region; Luminosity control module, for regulating and controlling the intensity of illumination of illumination lamp; Button time block, for carrying out timing to the light application time of described illumination lamp and the acquisition time of illuminance sensor illumination; Alarm module, for giving the alarm after described button time block timing terminates; LCD display module, for showing described intensity of illumination and light application time; And micro-chip, for illuminance sensor is transmitted illumination intensity value, button time block timing show in LCD display module, and when reaching timing control alarm module give the alarm; Described micro-chip is connected with illuminance sensor, luminosity control module, button time block, alarm module and LCD display module respectively;
Described temperature controlling system comprises: temperature sensor, for gathering the temperature value of illumination lamp irradiated region; Temperature raising device, heats for giving illumination lamp irradiated region; Refrigeration equipment, lowers the temperature for giving illumination lamp irradiated region; And temperature controller;
Described temperature controller comprises: display control module, for controlling temperature raising device and refrigeration equipment work by keying input mode, and the temperature variation of the temperature value of illumination lamp irradiated region, temperature raising device and refrigeration equipment is shown; And data processing module, for the keying instruction control temperature sensor according to display control module, temperature raising device and refrigeration equipment work, and show on display control module after the signal received is carried out data processing;
Described temperature controller is connected with temperature sensor, temperature raising device, refrigeration equipment respectively;
Described illumination lamp, temperature controller are connected with micro-chip respectively.
2. integrated SOD enzyme activity photoresponse experiment instrument as claimed in claim 1, it is characterized in that, this tester also comprises shell, and described shell comprises housing and lid; Install micro-chip, refrigeration equipment, temperature raising device and data processing module in described housing, described surface of shell installs button time block, alarm module, LCD display module and display control module; Be provided with reactive tank above described housing, install illumination lamp, temperature sensor and illuminance sensor in described reactive tank, the notch of described reactive tank installs lid.
3. integrated SOD enzyme activity photoresponse experiment instrument as claimed in claim 2, is characterized in that, be provided with bracket in described reactive tank, and described bracket is made up of the plate face of mesh and pillar stiffener, is fixed on reaction tank bottom, top support plate face bottom described pillar stiffener; Wherein, described illumination lamp, temperature sensor and illuminance sensor are positioned at below described plate face.
4. integrated SOD enzyme activity photoresponse experiment instrument as claimed in claim 3, it is characterized in that, described micro-chip is 80C51 micro-chip.
5. integrated SOD enzyme activity photoresponse experiment instrument as claimed in claim 4, it is characterized in that, described alarm module is hummer, and described LCD display module is LCD1602.
6. integrated SOD enzyme activity photoresponse experiment instrument as claimed in claim 5, it is characterized in that, described temperature raising device is magnetic sheet calorifier, and described refrigeration equipment is semiconductor air conditioner.
7. the integrated SOD enzyme activity photoresponse experiment instrument described in any one of claim 1 ~ 6 is in the application of SOD enzyme activity context of detection.
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| CN201510126121.5A CN104988061A (en) | 2015-03-23 | 2015-03-23 | SOD enzyme activity integrating light reaction experimental instrument and applications thereof |
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|---|---|---|---|
| CN201510126121.5A CN104988061A (en) | 2015-03-23 | 2015-03-23 | SOD enzyme activity integrating light reaction experimental instrument and applications thereof |
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| CN104988061A true CN104988061A (en) | 2015-10-21 |
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Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN87216728U (en) * | 1987-12-24 | 1988-10-26 | 北京工业学院 | Controlling instrument for physical and chemical experiment |
| CN2110225U (en) * | 1991-09-14 | 1992-07-15 | 北京中生生物工程高技术公司 | Semi-automatic biochemistry analysis instrument |
| JP2001074725A (en) * | 1999-09-09 | 2001-03-23 | Ishimekkusu:Kk | Method and apparatus for measuring concentration of organic substance |
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| CN101055242A (en) * | 2006-04-14 | 2007-10-17 | 中国科学院电子学研究所 | Portable dry method instrument for quickly detecting optical and biochemical parameters |
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