CN104988055A - Device for converting microalgae adherent culture and liquid submerged culture - Google Patents
Device for converting microalgae adherent culture and liquid submerged culture Download PDFInfo
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- CN104988055A CN104988055A CN201510478010.0A CN201510478010A CN104988055A CN 104988055 A CN104988055 A CN 104988055A CN 201510478010 A CN201510478010 A CN 201510478010A CN 104988055 A CN104988055 A CN 104988055A
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- liquid
- microalgae
- algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
Abstract
The invention provides a device for converting microalgae adherent culture and liquid submerged culture, and relates to a device for culturing microalgae. The problem that multiple and repeated conversions between the liquid submerged culture and the adherent culture cannot be achieved at present is mainly solved. The device comprises a concentrator, a communicating pipe, a drip irrigation pipe, a groove plate, a rotatable support and a collecting box. The device for converting the microalgae adherent culture and liquid submerged culture has the advantages that the cost is low, the operation is easy, and the maintenance is easy; the light decay phenomenon which is caused by mutual shielding of algae and exists in submerged culture is solved, and meanwhile the defects that the solubility of CO2 in a water body is lower, and the CO2 absorption efficiency is limited are overcome simultaneously; the conversion between the microalgae adherent culture and liquid submerged culture is achieved, the microalgae high carbon sequestration efficiency is achieved, and rapid and high-density culture is achieved simultaneously; the growth rate of microalgae is effectively improved, the culture cost of the microalgae is reduced, when the microalgae are cultured to reach the maximum biomass, the processes can reappear by placing the microalgae in the concentrator, and the purpose for converting repeatedly for multiple times is achieved.
Description
Technical field
The present invention relates to a kind of device cultivating micro-algae.
Background technology
Micro-algae can carry out putting oxygen, photosynthetic lower hydrobiont, and its structure is simple, and breeding rapidly, high to solar energy utilization ratio, there is the ability of GHG (Greenhouse Gases) emissions mitigation (carbonic acid gas), strong to environmental compatibility, be regarded as third generation biomass energy raw material.
Cultivate micro-algae many employings liquid submersion cultivate, water consumption is huge, and exist light propagates in water body occur decay and CO
2the problems such as the solubleness in water body is lower, limit the solid carbon efficiencies of chlorella and efficiency of light absorption.Adherent culture utilizes the adherent characteristic of frustule, decreases water consumption, makes CO
2can fully contact with frustule, CO
2utilization ratio significantly improve, and the conduction of light without the need to longer water body transmission, the efficiency of light energy utilization significantly improves, but micro algae growth rate is cultivated lower than liquid submersion.
Current wall-attaching type and liquid submersion training method are independently carry out substantially, do not occur that realizing liquid submersion cultivates the device repeatedly repeatedly changed between wall-attaching type cultivation.
Summary of the invention
The present invention seeks to solve the problem that cannot realize at present repeatedly repeatedly changing between liquid submersion cultivation with wall-attaching type cultivation, and a kind of microalgae adherence formula provided is cultivated and liquid submersion cultivates the device changed.
Microalgae adherence formula is cultivated and liquid submersion cultivates the device changed, and it comprises thickner, communicating pipe, drip irrigation pipe, concave-board, rotatable support frame, collection box; The placement direction of described concave-board is horizontal by the angle of 5-15 °; Described concave-board can not plate of material be made up of thoroughly the liquid permeable plate of material of upper surface flexibility and the liquid of lower surface rigidity; There is protruding structural unit on the liquid permeable plate of material surface of described upper surface flexibility, and the row of going and extending longitudinally extended transversely form building block array; The cross section of described structural unit is circular, square, rectangle, ellipse, trilateral or rhombus; Described structural unit in the same row equidistant from distance arranges or increases spacing successively; Line space between the adjacent lines of described building block array increases with constant increment or increment changes linearly; The described row extended transversely becomes same angle to extend in the horizontal direction, and the row extended longitudinally become same angle to extend in vertical direction.
Principle of work of the present invention: microalgae adherence formula provided by the invention is cultivated and liquid submersion cultivates the device changed, principle of work: the liquid containing micro-algae utilizes ultra-filtration technique to concentrate by thickner, then communicating pipe is flowed into, enter drip irrigation pipe again, micro-algae of flowing out through drip irrigation pipe is attached to concave-board upper surface, realize the transformation of liquid submersion cultivation conditions to wall-attaching type cultivation conditions, concussion is rocked through rotatable support frame, the micro-algae realizing being attached to concave-board upper surface revert to liquid submersion cultivation conditions by rocking concussion, collection box is in order to store the micro-algae come off from concave-board upper surface, realize the collection of algae liquid, when microdisk electrode is to most high-biomass, being placed in thickner can reproduce said process, realizes repetitious switch target.
Advantage of the present invention:
1, the device realizing the cultivation of microalgae adherence formula and liquid submersion cultivation conversion provided by the invention, cost is low, simple to operate, is easy to safeguard.
2, adopt device of the present invention to cultivate micro-algae, solve immersion and cultivate mutually covering due to algae of existing, the optical attenuation phenomenon caused, solves CO simultaneously
2solubleness in water body is lower, restriction CO
2the deficiency of assimilated efficiency.
3, adopt device of the present invention to cultivate micro-algae, solve the problem that the micro algae growth rate of wall-attaching type cultivation existence is slower.
4, device of the present invention is adopted to cultivate micro-algae, in the micro algae growth stage, achieve microalgae adherence formula to cultivate and liquid submersion cultivation conversion, give full play to the advantage that liquid submersion is cultivated and wall-attaching type is cultivated, while realizing the solid carbon efficiencies of micro-algae height, achieve fast high-density to cultivate, effectively improve the growth velocity of micro-algae, reduce the toxigenic capacity of micro-algae.
5, adopt device of the present invention to cultivate micro-algae, when microdisk electrode is to most high-biomass, being placed in thickner can reproduce said process, realizes repetitious switch target.
Accompanying drawing explanation
Fig. 1 is that in the present invention, microalgae adherence formula is cultivated and liquid submersion cultivates the structural representation of transfer equipment, 1 be thickner, 2 is wherein communicating pipe, 3 is drip irrigation pipe, 4 is concave-board, 5 be rotatable support frame, 6 is collection box;
Fig. 2 is that in the present invention, microalgae adherence formula is cultivated and the side view cutaway drawing of liquid submersion cultivation transfer equipment concave frid, and wherein the liquid of 7 to be the liquid permeable plate of material, 8 of upper surface flexibility be lower surface rigidity can not plate of material thoroughly;
Fig. 3 is the schematic diagram that in embodiment 1, the cultivation of microalgae adherence formula and liquid submersion cultivate transfer equipment concave frid upper surface, is wherein 9 structural units;
Fig. 4 is the schematic diagram that in embodiment 2, the cultivation of microalgae adherence formula and liquid submersion cultivate transfer equipment concave frid upper surface, is wherein 9 structural units.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: shown in composition graphs 1, Fig. 2, a kind of microalgae adherence formula of present embodiment is cultivated and liquid submersion cultivates the device changed, and it comprises thickner 1, communicating pipe 2, drip irrigation pipe 3, concave-board 4, rotatable support frame 5, collection box 6; The placement direction of described concave-board 4 is horizontal by the angle of 5-15 °; Described concave-board 4 can not plate of material 8 be made up of thoroughly the liquid permeable plate of material 7 of upper surface flexibility and the liquid of lower surface rigidity; There is protruding structural unit 9 on liquid permeable plate of material 7 surface of described upper surface flexibility, and the row of going and extending longitudinally extended transversely form building block array; The cross section of described structural unit 9 is circular, square, rectangle, ellipse, trilateral or rhombus; Described structural unit 9 in the same row equidistant from distance arranges or increases spacing successively; Line space between the adjacent lines of described building block array increases with constant increment or increment changes linearly; The described row extended transversely becomes same angle to extend in the horizontal direction, and the row extended longitudinally become same angle to extend in vertical direction.
Embodiment two: what present embodiment and embodiment one flowed out unlike, described thickner 1 exit is the algae liquid after concentrated, and the working pressure of thickner 1 is 0.10-0.20MPa.Other step and parameter identical with embodiment one.
Embodiment three: present embodiment and embodiment one or two unlike, described drip irrigation pipe 3, be responsible for upper surface algae liquid being laid in concave-board 4, realize the transformation of liquid submersion cultivation conditions to wall-attaching type cultivation conditions, the nominal diameter of drip irrigation pipe 3 is 12-16mm.Other step and parameter identical with embodiment one or two.
Embodiment four: one of present embodiment and embodiment one to three are placed in highest point unlike, described concave-board 4 one section of being placed with drip irrigation pipe 3, and the speed that drip irrigation pipe 3 flows out algae liquid is 0.008ml/s.Other step and parameter identical with one of embodiment one to three.
Embodiment five: one of present embodiment and embodiment one to four unlike, the two ends of described rotatable support frame 5 are connected to concave-board 4 and collection box 6, in order to realize the rotation of anchor frid 4, and the micro-algae being attached to concave-board 4 upper surface is revert to liquid submersion cultivation conditions by rocking concussion.Other step and parameter identical with one of embodiment one to four.
Embodiment six: one of present embodiment and embodiment one to five unlike, the rotating speed of described rotatable support frame 5 is 100-300r/min, and material is 301 stainless steels, 304 stainless steels, 312 stainless steels or 316 stainless steels.Other step and parameter identical with one of embodiment one to five.
Embodiment seven: one of present embodiment and embodiment one to six are unlike, described collection box 6 in order to store the micro-algae come off from concave-board 4 upper surface, and realize the collection of algae liquid, long-width ratio is (1-8): 1, and height is 0.2-0.35m.Other step and parameter identical with one of embodiment one to six.
Embodiment eight: one of present embodiment and embodiment one to seven unlike, the height of described structural unit 9 is 5-20mm.Other step and parameter identical with one of embodiment one to seven.
Embodiment nine: one of present embodiment and embodiment one to eight unlike, described, the modular construction 9 in the row extended longitudinally has identical size.Other step and parameter identical with one of embodiment one to eight.
Embodiment ten: one of present embodiment and embodiment one to nine unlike, described in the size of modular construction 9 in the row that extend longitudinally can also to reduce with constant decrease or decrease change linearly.Other step and parameter identical with one of embodiment one to nine.
Embodiment 11: one of present embodiment and embodiment one to nine are that one or more materials in glass fibre, nylon, cotton, fiber crops, carbon fiber, synthon, sponge, plastic foam are made unlike the material of, the liquid permeable plate of material 7 of described upper surface flexibility.
In present embodiment, material is that multiple material is mixed by any ratio when making.
Following examples are adopted to verify beneficial effect of the present invention:
Embodiment 1:
Shown in composition graphs 1, Fig. 2 and Fig. 3, adopt microalgae adherence formula provided by the invention cultivation and liquid submersion to cultivate the device of conversion, wherein the working pressure of thickner 1 is 0.15MPa; The nominal diameter of drip irrigation pipe 3 is 16mm; The speed that drip irrigation pipe 3 flows out algae liquid is 0.008ml/s; The material of the liquid permeable plate of material 7 of upper surface flexibility is glass fibre, is of a size of 300mm × 300mm; The cross section of structural unit 9 is circular, is highly 5mm.
In incubator, algae liquid amasss as 500L, and starting point concentration is 1 × 10
9individual/ml, after thickner 1 concentrates 3 hours, concentration becomes 7.342 × 10
10individual/ml, concentrated solution is 6.8L; Concave-board 4 upper surface is paved with after 1 hour; Collected with the lower concentration algae liquid slided from concave-board 4 in titration process by concentrated solution and mix, cumulative volume is about 500L, and recording concentration of algae is 6.3 × 10
7individual/ml.
The ratio that the algae amount be attached on concave-board 4 accounts for original total algae amount is:
Cultivate after 1 day, cleaned by the lower concentration algae liquid that the algae that concave-board 4 attaches puts into collection, rotating speed is 150r/min, and the concentration measuring algae is 4.5 × 10
9individual/ml, algae liquid cumulative volume is 499L, and volume change is little.
The increased times of algae is:
Visible, adopt apparatus of the present invention can improve the growth velocity of micro-algae, ideal adherent effect.
Embodiment 2:
Shown in composition graphs 1, Fig. 2 and Fig. 4, adopt microalgae adherence formula provided by the invention cultivation and liquid submersion to cultivate the device of conversion, wherein the working pressure of thickner 1 is 0.15MPa; The nominal diameter of drip irrigation pipe 3 is 16mm; The speed that drip irrigation pipe 3 flows out algae liquid is 0.008ml/s; The material of the liquid permeable plate of material 7 of upper surface flexibility is glass fibre, is of a size of 300mm × 300mm; The cross section of structural unit 10 is rhombus, is highly 6mm.
The ratio that the algae amount measured on concave-board 4 accounts for original total algae amount is:
Cultivate after 1 day, cleaned by the lower concentration algae liquid that the algae that concave-board 4 attaches puts into collection, the concentration measuring algae is 3.0/ml, and algae liquid cumulative volume is 499L, and volume change is little.
The increased times of algae is:
Visible, adopt apparatus of the present invention can improve the growth velocity of micro-algae, ideal adherent effect.
Claims (10)
1. the device that microalgae adherence formula is cultivated and liquid submersion cultivation is changed, is characterized in that it comprises thickner (1), communicating pipe (2), drip irrigation pipe (3), concave-board (4), rotatable support frame (5), collection box (6); The placement direction of described concave-board (4) is horizontal by the angle of 5-15 °; Described concave-board (4) can not plate of material (8) be made up of thoroughly the liquid permeable plate of material (7) of upper surface flexibility and the liquid of lower surface rigidity; There is protruding structural unit (9) on liquid permeable plate of material (7) surface of described upper surface flexibility, and the row of going and extending longitudinally extended transversely form building block array; The cross section of described structural unit (9) is circular, square, rectangle, ellipse, trilateral or rhombus; Described structural unit (9) in the same row equidistant from distance arranges or increases spacing successively; Line space between the adjacent lines of described building block array increases with constant increment or increment changes linearly; The described row extended transversely becomes same angle to extend in the horizontal direction, and the row extended longitudinally become same angle to extend in vertical direction.
2. the device that a kind of microalgae adherence formula according to claim 1 is cultivated and liquid submersion cultivation is changed, what it is characterized in that described thickner (1) exit flows out is the algae liquid after concentrated, and the working pressure of thickner (1) is 0.10-0.20MPa.
3. the device that a kind of microalgae adherence formula according to claim 1 is cultivated and liquid submersion cultivation is changed, it is characterized in that described drip irrigation pipe (3), be responsible for upper surface algae liquid being laid in concave-board (4), realize the transformation of liquid submersion cultivation conditions to wall-attaching type cultivation conditions, the nominal diameter of drip irrigation pipe (3) is 12-16mm.
4. the device that a kind of microalgae adherence formula according to claim 1 is cultivated and liquid submersion cultivation is changed, it is characterized in that described concave-board (4) is placed with a section of drip irrigation pipe (3) and is placed in highest point, the speed that drip irrigation pipe 3 flows out algae liquid is 0.008ml/s.
5. the device that a kind of microalgae adherence formula according to claim 1 is cultivated and liquid submersion cultivation is changed, it is characterized in that the two ends of described rotatable support frame (5) are connected to concave-board (4) and collection box (6), in order to realize the rotation of anchor frid (4), and the micro-algae being attached to concave-board (4) upper surface is revert to liquid submersion cultivation conditions by rocking concussion.
6. the device that a kind of microalgae adherence formula according to claim 1 is cultivated and liquid submersion cultivation is changed, it is characterized in that the rotating speed of described rotatable support frame (5) is 100-300r/min, material is 301 stainless steels, 304 stainless steels, 312 stainless steels or 316 stainless steels.
7. the device that a kind of microalgae adherence formula according to claim 1 is cultivated and liquid submersion cultivation is changed, it is characterized in that described collection box (6) is in order to store the micro-algae come off from concave-board (4) upper surface, realize the collection of algae liquid, long-width ratio is (1-8): 1, and height is 0.2-0.35m.
8. the device that a kind of microalgae adherence formula according to claim 1 is cultivated and liquid submersion cultivation is changed, is characterized in that the height of described structural unit (9) is 5-20mm.
9. the device that a kind of microalgae adherence formula according to claim 1 is cultivated and liquid submersion cultivation is changed, the modular construction 9 in the row then extended longitudinally described in it is characterized in that has identical size.
10. the device that a kind of microalgae adherence formula according to claim 1 is cultivated and liquid submersion cultivation is changed, the size of the modular construction (9) in the row extended longitudinally described in it is characterized in that can also reduce with constant decrease or decrease change linearly.
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| CN201510478010.0A CN104988055B (en) | 2015-08-06 | 2015-08-06 | A kind of device of the culture of microalgae adherence formula and liquid submersion culture conversion |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110903947A (en) * | 2019-12-20 | 2020-03-24 | 深圳泰利能源有限公司 | Open type photobioreactor for microalgae cultivation |
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| US4908128A (en) * | 1987-09-15 | 1990-03-13 | Envirocycle Pty. Ltd. | Composite bacteria support medium |
| US6664095B1 (en) * | 1998-11-17 | 2003-12-16 | Biocon India Limited | Solid state fermentation |
| CN101870950A (en) * | 2009-04-24 | 2010-10-27 | 中国石油化工股份有限公司 | Device for culturing microalgae |
| CN103289888A (en) * | 2012-03-01 | 2013-09-11 | 中国科学院青岛生物能源与过程研究所 | Inserting-plate type microalgae semi-dry solid adherent culture device |
| CN103396936A (en) * | 2013-07-30 | 2013-11-20 | 新奥科技发展有限公司 | Light biological cultivation unit and cultivation system |
| CN104328048A (en) * | 2014-10-30 | 2015-02-04 | 国家开发投资公司 | Surface growth type photosynthetic microorganism culture plate and surface growth type photosynthetic microorganism culture system |
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- 2015-08-06 CN CN201510478010.0A patent/CN104988055B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4908128A (en) * | 1987-09-15 | 1990-03-13 | Envirocycle Pty. Ltd. | Composite bacteria support medium |
| US6664095B1 (en) * | 1998-11-17 | 2003-12-16 | Biocon India Limited | Solid state fermentation |
| CN101870950A (en) * | 2009-04-24 | 2010-10-27 | 中国石油化工股份有限公司 | Device for culturing microalgae |
| CN103289888A (en) * | 2012-03-01 | 2013-09-11 | 中国科学院青岛生物能源与过程研究所 | Inserting-plate type microalgae semi-dry solid adherent culture device |
| CN103396936A (en) * | 2013-07-30 | 2013-11-20 | 新奥科技发展有限公司 | Light biological cultivation unit and cultivation system |
| CN104328048A (en) * | 2014-10-30 | 2015-02-04 | 国家开发投资公司 | Surface growth type photosynthetic microorganism culture plate and surface growth type photosynthetic microorganism culture system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110903947A (en) * | 2019-12-20 | 2020-03-24 | 深圳泰利能源有限公司 | Open type photobioreactor for microalgae cultivation |
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