CN104987225A - Culture medium material for cultivating Morchella conica by using cellulosic ethanol waste residue and preparation method therefor - Google Patents
Culture medium material for cultivating Morchella conica by using cellulosic ethanol waste residue and preparation method therefor Download PDFInfo
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Abstract
The invention relates to the technical field of preparation of culture medium materials for edible fungi. A culture medium material for cultivating Morchella conica by using cellulosic ethanol waste residue is prepared from the cellulosic ethanol waste residue, plant organics and auxiliaries. According to the invention, the culture medium material is prepared by a simple preparation process in a manner that cellulosic ethanol, which serves as a main material, is matched with appropriate plant organics and auxiliaries. According to the invention, the cost is reduced, the utilization ratio of the cellulosic ethanol waste residue is increased, and the wasting of resources is reduced; and through cultivating Morchella conica by using the culture medium material provided by the invention, the fruiting stage of Morchella conica can be prolonged, and the yield of Morchella conica is increased.
Description
Technical field
The present invention relates to culture medium of edible fungus material manufacture technology field, particularly relate to a kind of cellulose ethanol waste residue that utilizes and cultivate culture base-material of morel and preparation method thereof.
Background technology
Morel (Morchellaconica) has another name called delicious morel, be commonly called as sheep sparrow bacterium, sheep tripe dish, maize bacterium etc., being under the jurisdiction of Ascomycotina, Ascomycetes, Pezizale, Morchellaceae, morchella by categorizing system, is one of foremost rare edible medicinal fungus in the world.Its delicious flavour, nutritious, its sporophore rich in proteins, polysaccharide, nucleic acid, various trace elements and VITAMIN, the 7 kinds of indispensable amino acids wherein having human body to need, have unique health-care effect, are the edible mushroomss of old and young all benefits.In China, the medicinal record of morel starts from the Compendium of Material Medica of LI Shi-Zhen, " sweet cold nontoxic, beneficial stomach, phlegm-eliminiating and qi-regulating ", polysaccharide etc. are the main effective medicinal components of morel, have multiple efficacies such as strengthening immunologic function, antifatigue, anti-ageing, antitumor, antimutagenic, reducing blood-fat, prevention of arterial sclerosis and flu.
Although morel has important edible and pharmaceutical use, but because wild toadstool requires very high to growing environment, China's wild toadstool annual production is about 10t, and the national annual production only about 300t of artificial bionic cultivation, China in Recent Years market morel price is basically stable at 1600 ~ 2000 yuan/kg, product main exit is external, and supply falls short of demand, has very large market development prospect.Its artificial domesticating cultivation is the problem that domestic and international fungus scholar endeavours research and probe always, current morel factorial praluction technology is also immature, all utilize Emulational culture mostly, obtain the cultivation being applicable to local environment, the morel product consistent with the local wild toadstool season of growth, it is also just effective that semi-artificial planting patterns imitative wild in recent years has been risen gradually.
Nanyang City, Henan Province Tian Guan group has a large amount of cellulose ethanol production line, it utilizes maize straw to produce ethanol, 400t cellulose ethanol waste residue can be produced every day, cellulose ethanol waste residue closes a large amount of organism, its composition formation comprises through assay: Mierocrystalline cellulose 21.32wt%, hemicellulose 2.43wt%, xylogen 52.12wt%, ash content 23.9wt%, wherein xylogen is the main component in cellulose ethanol waste residue.Not yet find both at home and abroad at present to utilize cellulose ethanol waste residue to cultivate the research report of the culture base-material of morel and preparation method thereof.
CN 101508599 B discloses a kind of method being raw material production high-quality edible mushroom culture medium with manioc waste and acid-sludge; By by composite for the tankage science of manioc waste and the common fermentation of acid-sludge two kinds or foodstuffs industry, as production high-quality edible mushroom culture medium, can be applied in the production of all culture medium of edible fungus.CN 103880538 A discloses culture base-material of a kind of morel and preparation method thereof, and culture base-material is made up of following component: peat, residue from beans after making, pomelo peel, tomato seedling, plant ash, potassium primary phosphate, urea, terra alba, unslaked lime and conditioning agent; The preparation method of culture base-material, comprises the following steps: (1) gets the raw materials ready; (2) pulverize; (3) mix; (4) ferment; (5) feed.The culture base-material of the morel that this invention provides, on the basis of conventional medium material, add peat and multiple waste, not only meet the nutritional needs of morel growth, also utilize mycelia to produce, and material source is extensive, cost is lower.The saprophytic edible mushrooms of morchella low temperature and high relative humidity type, the culture base-material cost of existing conventional cultivation morel is high, send out bacterium speed slow, growth cycle is long, send out a bacterium yields poorly, and it is short etc. to adopt the mushroom phase, along with the development of social city, technicalization, hommization, a kind of the bacterium time of design is short, produce cellulose ethanol residue culture medium material of mushroom time length and preparation method thereof, to meet the need of market, be very important.
Summary of the invention
Technical problem to be solved by this invention is, high for existing culture base-material cultivation morel cost, send out that the bacterium time is long, the production cycle is long, adopt the technical problems such as the mushroom phase is short, provide a kind of cellulose ethanol waste residue that utilizes to cultivate culture base-material of morel and preparation method thereof.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is:
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the raw material of following weight part: the auxiliary material of the cellulose ethanol waste residue of 35 ~ 60 weight parts, the plant organic matter of 35 ~ 55 weight parts, 3 ~ 5 weight parts.
Preferably, described plant organic matter is one or more the composition in cotton seed hulls, husk, Pericarppium arachidis hypogaeae, wheat bran, corn cob, agricultural crop straw, branch, leaf.
Preferably, described agricultural crop straw is
wheatstalk, rice straw,
cornstalk, potato class stalk,
cottonstalk,
rapestalk, peanut straw,
sugarcaneone or more compositions in stalk.
Preferably, described auxiliary material is the customary adjuvant in morel cultivation; Preferably, described auxiliary material is selected from one or more combinations in gypsum, lime, calcium superphosphate, potassium primary phosphate, magnesium sulfate, VITAMIN.
Preferably, the described culture base-material utilizing cellulose ethanol waste residue to cultivate morel, is prepared by the following raw material of weight part: cellulose ethanol waste residue 35 ~ 60 weight part, corn cob 2.5 ~ 7.5 weight part, cotton seed hulls 12.5 ~ 17.5 weight part, wheat bran 10 ~ 15 weight part, husk 10 ~ 15 weight part, lime 2 weight part, calcium superphosphate 1 weight part.
Utilize cellulose ethanol waste residue to cultivate a preparation method for the culture base-material of morel, comprise the following steps:
1) get the raw materials ready: each raw material preparing and take above-mentioned weight part respectively;
2) mix: mixed by the above-mentioned each raw material taken, and stir, add water the water content and pH value that regulate mixture, obtains wet feed;
3) pack: will through above-mentioned steps gained wet feed, adopt artificial or mechanical system to load in the packing bag making morel substratum, obtain pocket;
4) sterilizing: above-mentioned pocket is adopted normal-pressure sterilization, is rapidly heated to 100 ± 2 DEG C, maintains 20h.
Preferably, described cellulose ethanol waste residue is dry, pulverizing, makes it granularity and is not more than 1 centimetre.
Preferably, described cellulose ethanol waste residue and cotton seed hulls are sprayed water the 10h that prewets before combination, and described corn cob and husk are sprayed water the 5h that prewets before compound.
Preferably, in described mixing step, the water content control of described wet feed is at 60 ~ 65wt%, and pH value is 6.5 ~ 7.5.
Preferably, in described pouch step, in described each packing bag, fill wet feed 1.0 ~ 1.2kg, wherein containing siccative 0.5 ~ 0.6kg.
Preferably, described packing bag adopts polypropylene plastics pocket; Preferred, described packing bag employing specification is the polypropylene plastics pocket of 17cm × 38cm × 0.05cm or 20cm × 33cm × 0.05cm; In bagging process, note degree of tightness appropriateness, pack complete, adopt Plastibell sealing.
Utilize cellulose ethanol waste residue to cultivate a method for the culture base-material cultivation morel of morel, comprise the following steps:
1) inoculation fermentation: the substratum pocket temperature after sterilizing is down to 20 ± 2 DEG C, then moves in transfer room, inoculates by aseptic technique code; After inoculation, the temperature that controls environment 18 ~ 22 DEG C, control environment humidity 70% ~ 80%, and cultivate 30 ~ 35 days, namely Morciiella Esculeuta Mycelia covers with pocket, obtains cultivation strain;
2) management of producing mushroom: cultivation strain is moved in the cultivation place put in order, plant ash and wheat are first spread in the ground of cultivation place, broadcast cultivation strain again, the last thin vegetable mould covering upper about 3cm above, keep the humidity and temperature that culture material is suitable, after morel is unearthed, color becomes light grey or isabelline can gathering from Dark grey.
Described plant ash every mu spreads 150 ~ 200g, is used for regulating soil acidity or alkalinity, increases the content of P, K in soil simultaneously.
Described per mu spreads 0.5Kg, is mainly used to accompany bacteria growing, simultaneously water conservation, sunshade.
The cellulose ethanol waste residue adopted in the present invention, the waste residue that the cellulose ethanol production line preferably deriving from Nanyang City, Henan Province Tian Guan group produces, the composition formation of this waste residue comprises through assay: Mierocrystalline cellulose 21.32wt%, hemicellulose 2.43wt%, xylogen 52.12wt%, total nitrogen 2.49wt%, ash content 21.64wt%, the outstanding advantage of this waste residue is that nutritive ingredient is high.From value, raw material is stablized, and draws materials conveniently, decreases transport and the handling cost of raw material.Because cellulose ethanol waste particle is little, ventilation property is poor, the plant organic matter of good permeability need be coordinated to solve ventilatory problem, plant organic matter comprises cotton seed hulls, husk, Pericarppium arachidis hypogaeae, wheat bran, corn cob, agricultural crop straw, branch, leaf etc. simultaneously, equal wide material sources, they are generally byproduct or waste, are used as the culture base-material composition of morel, can also turn waste into wealth.
Corn cob uses
corn earthreshing processing is made through screening again, has homogeneous microstructure, hardness is suitable, good toughness, water-absorbent strong, the easy advantage such as broken; Required nutritive ingredient is produced required for the morels such as crude protein, robust fibre, carbohydrate, crude fat, mineral substance.Cotton seed hulls also claims cotton skin, is the shell that cottonseed is left after decorticator is separated, the crude protein usually containing 5 ~ 8wt%, before using, needs first to reject the rotten cotton seed hulls that goes rotten, is then crushed to suitable granularity.Wheat bran is gained byproduct after wheat processing flour, and be typically used as feed and use, economic worth is not high; Wherein can for the nutritive substance of morel growth containing Mierocrystalline cellulose, protein, VITAMIN, growth hormone etc.Husk is generally the byproduct of Rice producing process, rich cellulose in husk,
xylogenand silicon-dioxide, the content of fat and protein is extremely low, has the features such as good toughness, porousness, low density and quality are coarse.The pH of cellulose ethanol waste residue is generally 5.0 ~ 5.5, slant acidity, during lime used is used in the present invention and the acidity of cellulose ethanol waste residue, makes it reach suitable pH value.
Known, the saprophytic edible mushrooms of morchella low temperature and high relative humidity type, they are different from general club fungi, are the ascomycetess in fungi, and required condition of growing is also more special, so manually lower in the operability of cultivation.The nutritive ingredient such as carbon source, nitrogenous source, inorganic salt needed for morel growth, and the envrionment conditions such as temperature, humidity, potential of hydrogen, have significant difference with other edible mushrooms.The source of above-mentioned nutritive ingredient and content thereof, have very important impact to the upgrowth situation of morel, if nutritive ingredient is excessive, heavy then cause morel cannot normal growth, nutritive ingredient deficiency then can cause later stage nutrition supply not enough.Therefore, need to consider the synergy between each feed composition when selecting composition of raw materials.Secondly, the strict growing environment controlling morel is needed.Morel is very strict to the requirement of its production environment, therefore, in the preparation and cultivation of culture base-material, needs specific temperature, humidity and pH value etc.The present invention puts into practice in a large number and shows, the water content control of substratum wet feed is at 60 ~ 65wt%, and pH value is 6.5 ~ 7.5, the most favourable to the growth of Morciiella Esculeuta Mycelia; The temperature that controls environment in cultivation 18 ~ 22 DEG C, control environment humidity 70% ~ 80%, the most favourable to the fruiting of morel, by the optimization to culture base-material parameter and planting environment parameter, can realize controlling cost low, the effect that output is high.
The culture base-material utilizing cellulose ethanol waste residue to cultivate morel provided by the invention, using cellulose ethanol waste residue as major ingredient, not only reduce cost, also improve the utilization ratio of cellulose ethanol waste, decrease the waste of resource, and the morel utilizing culture base-material provided by the invention to cultivate, the morel fruiting phase can be extended, improving morel output, is ideal morel culture base-material.
Found by the continuous exploratory development of nearly 3 years, utilize cellulose ethanol waste residue feasible as morel culturing raw material, not only have with low cost, send out bacterium speed fast, the object of increasing economic efficiency, simultaneously, change the fermentation residue produced in cellulose ethanol production process usually to be burnt by as fuel, economic benefit is not high, and the predicament of contaminate environment, and the efficient circulation for cellulose ethanol waste residue utilizes walks out a new way.
Beneficial effect of the present invention is as follows:
1) raw material of the present invention is easy to get, wide material sources, cellulose ethanol waste residue, plant organic matter etc. is turned waste into wealth, improves its utility value, economize on resources, simultaneously for the comprehensive utilization of cellulose ethanol waste residue provides a new thinking.
2) Raw composition of the present invention and reasonable ratio, Composition Control is strict, and the growth for Morciiella Esculeuta Mycelia provides favourable developing environment, while guarantee nutrition, can promote the rapidly and efficiently ripe of Morciiella Esculeuta Mycelia.
3) the present invention is in conjunction with raw materials feature, provides the culture base-material preparation method utilizing cellulose ethanol waste residue to cultivate morel, and preparation method's step of the present invention is reasonable, and state modulator is suitable for, and the high yield for Morciiella Esculeuta Mycelia grows fast and provides favourable guarantee.
4) compared with prior art, morel of the present invention buddings fruiting time advance more than 1 week, and the fruiting phase extends more than 1 week, output increased 24.2% ~ 39.02%.
Embodiment
In order to understand the present invention further, below in conjunction with embodiment, the preferred embodiment of the invention is described, but should be appreciated that these describe just for further illustrating the features and advantages of the present invention, instead of limiting to the claimed invention.
Embodiment 1
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the following raw material of weight part: cellulose ethanol waste residue 45 weight part, corn cob 7.5 weight part, cotton seed hulls 17.5 weight part, wheat bran 13.5 weight part, husk 14.5 weight part, lime 2 weight part, calcium superphosphate 1 weight part.
The above-mentioned preparation method utilizing cellulose ethanol waste residue to cultivate the culture base-material of morel, carries out according to the following steps:
(1) get the raw materials ready: each raw material preparing and take above-mentioned weight part;
(2) mix: mixed by the above-mentioned each raw material taken, and stir, add water the water content and pH value that regulate mixture, and maintenance water content is 63wt%, and pH value is 6.8.The screening of cellulose ethanol waste residue, fully drying; Cellulose ethanol waste residue and cotton seed hulls should be sprayed water the 10h that prewets before combination, and corn cob and husk are prewetted 5h;
(3) pack: employing specification is the polypropylene plastics pocket of 17cm × 38cm × 0.05cm, artificial or machinery pack, every packed siccative galassing all about 0.51kg, make cylindrical, namely culture base-material is made;
(4) sterilizing: pack complete, Plastibell seals; Adopt conventional atmospheric sterilizing, be rapidly heated to 100 ± 2 DEG C, maintain this temperature 20h.
Utilize above-mentioned culture base-material to carry out the method for morel cultivation, can carry out according to the following steps:
1) inoculation fermentation: the substratum pocket temperature after sterilizing is down to 20 ± 2 DEG C, then moves in transfer room, inoculates by aseptic technique code; After inoculation, the temperature that controls environment 20 DEG C, control environment humidity 74%, and cultivate 30 days, namely Morciiella Esculeuta Mycelia covers with pocket, obtains cultivation strain;
2) management of producing mushroom: cultivation strain is moved in the cultivation place put in order, plant ash and wheat are first spread in the ground of cultivation place, broadcast cultivation strain again, the last thin vegetable mould covering upper about 3cm above, keep the humidity and temperature that culture material is suitable, after morel is unearthed, color becomes light grey or isabelline can gathering from Dark grey.
Wherein, described plant ash every mu spreads 180g, is used for regulating soil acidity or alkalinity, increases the content of P, K in soil simultaneously; Described per mu spreads 0.5Kg, is mainly used to accompany bacteria growing, simultaneously water conservation, sunshade.
Embodiment 2
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the following raw material of weight part: cellulose ethanol waste residue 50 weight part, corn cob 5.5 weight part, cotton seed hulls 15.5 weight part, wheat bran 15 weight part, husk 13 weight part, lime 2 weight part, calcium superphosphate 1 weight part.
The above-mentioned preparation method utilizing cellulose ethanol waste residue to cultivate the culture base-material of morel, carries out according to the following steps:
(1) get the raw materials ready: each raw material preparing and take above-mentioned weight part;
(2) mix: mixed by the above-mentioned each raw material taken, and stir, add water the water content and pH value that regulate mixture, and maintenance water content is 62.6wt%, and pH value is 6.7.The screening of cellulose ethanol waste residue, fully drying; Cellulose ethanol waste residue and cotton seed hulls should be sprayed water the 10h that prewets before combination, and corn cob and husk are prewetted 5h;
(3) pack: employing specification is the polypropylene plastics pocket of 20cm × 33cm × 0.05cm, artificial or machinery pack, every packed wet feed is containing siccative on average heavily about 0.58kg, and make cylindrical, namely culture base-material is made;
(4) sterilizing: pack complete, Plastibell seals; Adopt conventional atmospheric sterilizing, be rapidly heated to 100 ± 2 DEG C, maintain this temperature 20h.
Utilize above-mentioned culture base-material to carry out the method for morel cultivation, can carry out according to the following steps:
1) inoculation fermentation: the substratum pocket temperature after sterilizing is down to 20 ± 2 DEG C, then moves in transfer room, inoculates by aseptic technique code; After inoculation, the temperature that controls environment 18 DEG C, control environment humidity 80%, and cultivate 32 days, namely Morciiella Esculeuta Mycelia covers with pocket, obtains cultivation strain;
2) management of producing mushroom: cultivation strain is moved in the cultivation place put in order, plant ash and wheat are first spread in the ground of cultivation place, broadcast cultivation strain again, the last thin vegetable mould covering upper about 3cm above, keep the humidity and temperature that culture material is suitable, after morel is unearthed, color becomes light grey or isabelline can gathering from Dark grey.
Wherein, described plant ash every mu spreads 150g, is used for regulating soil acidity or alkalinity, increases the content of P, K in soil simultaneously; Described per mu spreads 0.5Kg, is mainly used to accompany bacteria growing, simultaneously water conservation, sunshade.
Embodiment 3
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the following raw material of weight part: cellulose ethanol waste residue 55 weight part, corn cob 2.5 weight part, cotton seed hulls 12.5 weight part, wheat bran 15 weight part, husk 13 weight part, lime 2 weight part, calcium superphosphate 1 weight part.
The above-mentioned preparation method utilizing cellulose ethanol waste residue to cultivate the culture base-material of morel, carries out according to the following steps:
(1) get the raw materials ready: each raw material preparing and take above-mentioned weight part;
(2) mix: mixed by the above-mentioned each raw material taken, and stir, add water the water content and pH value that regulate mixture, and maintenance water content is 64wt%, and pH value is 7.1.The screening of cellulose ethanol waste residue, fully drying; Cellulose ethanol waste residue and cotton seed hulls should be sprayed water the 10h that prewets before combination, and corn cob and husk are prewetted 5h;
(3) pack: employing specification is the polypropylene plastics pocket of 17cm × 38cm × 0.05cm, artificial or machinery pack, often packed containing siccative heavily about 0.53kg, make cylindrical, namely culture base-material is made;
(4) sterilizing: pack complete, Plastibell seals.Adopt conventional atmospheric sterilizing, be rapidly heated to 100 ± 2 DEG C, maintain this temperature 20h.
Utilize above-mentioned culture base-material to carry out the method for morel cultivation, can carry out according to the following steps:
1) inoculation fermentation: the substratum pocket temperature after sterilizing is down to 20 ± 2 DEG C, then moves in transfer room, inoculates by aseptic technique code; After inoculation, the temperature that controls environment 22 DEG C, control environment humidity 70%, and cultivate 35 days, namely Morciiella Esculeuta Mycelia covers with pocket, obtains cultivation strain;
2) management of producing mushroom: cultivation strain is moved in the cultivation place put in order, plant ash and wheat are first spread in the ground of cultivation place, broadcast cultivation strain again, the last thin vegetable mould covering upper about 3cm above, keep the humidity and temperature that culture material is suitable, after morel is unearthed, color becomes light grey or isabelline can gathering from Dark grey.
Wherein, described plant ash every mu spreads 200g, is used for regulating soil acidity or alkalinity, increases the content of P, K in soil simultaneously; Described per mu spreads 0.5Kg, is mainly used to accompany bacteria growing, simultaneously water conservation, sunshade.
Embodiment 4
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the following raw material of weight part: cellulose ethanol waste residue 35 weight part, corn cob 7.5 weight part, cotton seed hulls 17.5 weight part, wheat bran 15 weight part, husk 15 weight part, lime 2 weight part, calcium superphosphate 1 weight part.
The above-mentioned preparation method utilizing cellulose ethanol waste residue to cultivate the culture base-material of morel, carries out according to the following steps:
(1) get the raw materials ready: each raw material preparing and take above-mentioned weight part;
(2) mix: mixed by the above-mentioned each raw material taken, and stir, add water the water content and pH value that regulate mixture, and maintenance water content is 60wt%, and pH value is 6.5.The screening of cellulose ethanol waste residue, fully drying; Cellulose ethanol waste residue and cotton seed hulls should be sprayed water the 10h that prewets before combination, and corn cob and husk are prewetted 5h;
(3) pack: employing specification is the polypropylene plastics pocket of 17cm × 38cm × 0.05cm, artificial or machinery pack, every packed siccative galassing all about 0.50kg, make cylindrical, namely culture base-material is made;
(4) sterilizing: pack complete, Plastibell seals; Adopt conventional atmospheric sterilizing, be rapidly heated to 100 ± 2 DEG C, maintain this temperature 20h.
Above-mentioned culture base-material is utilized to carry out the method for morel cultivation, with embodiment 1.
Embodiment 5
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the following raw material of weight part: cellulose ethanol waste residue 60 weight part, corn cob 6.5 weight part, cotton seed hulls 12.5 weight part, wheat bran 10 weight part, husk 10 weight part, lime 2 weight part, calcium superphosphate 1 weight part.
The above-mentioned preparation method utilizing cellulose ethanol waste residue to cultivate the culture base-material of morel, carries out according to the following steps:
(1) get the raw materials ready: each raw material preparing and take above-mentioned weight part;
(2) mix: mixed by the above-mentioned each raw material taken, and stir, add water the water content and pH value that regulate mixture, and maintenance water content is 65wt%, and pH value is 7.5.The screening of cellulose ethanol waste residue, fully drying; Cellulose ethanol waste residue and cotton seed hulls should be sprayed water the 10h that prewets before combination, and corn cob and husk are prewetted 5h;
(3) pack: employing specification is the polypropylene plastics pocket of 20cm × 33cm × 0.05cm, artificial or machinery pack, every packed siccative galassing all about 0.60kg, make cylindrical, namely culture base-material is made;
(4) sterilizing: pack complete, Plastibell seals; Adopt conventional atmospheric sterilizing, be rapidly heated to 100 ± 2 DEG C, maintain this temperature 20h.
Above-mentioned culture base-material is utilized to carry out the method for morel cultivation, with embodiment 2.
Embodiment 6
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the following raw material of weight part: cellulose ethanol waste residue 55 weight part, cotton seed hulls 11.5 weight part, wheat bran 14 weight part, husk 13 weight part, maize straw 3.5 weight part, leaf 1 weight part, lime 2 weight part, calcium superphosphate 1 weight part.
The above-mentioned preparation method utilizing cellulose ethanol waste residue to cultivate the culture base-material of morel, with implementing 1.
Above-mentioned culture base-material is utilized to carry out the method for morel cultivation, with embodiment 3.
Embodiment 7
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the following raw material of weight part: cellulose ethanol waste residue 50 weight part, cotton seed hulls 12.5 weight part, husk 15 weight part, Pericarppium arachidis hypogaeae 6.5 weight part, agricultural crop straw 4.5 weight part, corn cob 7.5 weight part, leaf 2 weight part, magnesium sulfate 1 weight part, calcium superphosphate 1 weight part, gypsum 1 weight part, lime 2 weight part.Wherein, agricultural crop straw is selected from
wheatstalk, rice straw,
cornstalk, potato class stalk,
cottonstalk,
rapestalk, peanut straw,
sugarcaneone or more compositions in stalk, if when it is multiple combination thing, can proportioning arbitrarily.
The above-mentioned preparation method utilizing cellulose ethanol waste residue to cultivate the culture base-material of morel, with implementing 2.
Above-mentioned culture base-material is utilized to carry out the method for morel cultivation, with embodiment 1.
Embodiment 8
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the following raw material of weight part: cellulose ethanol waste residue 45 weight part, cotton seed hulls 13.5 weight part, husk 12 weight part, wheat bran 11.5 weight part, Pericarppium arachidis hypogaeae 7.5 weight part, corn cob 7.5 weight part, branch 1 weight part, potassium primary phosphate 1 weight part, lime 2 weight part, VITAMIN 1 weight part.
The above-mentioned preparation method utilizing cellulose ethanol waste residue to cultivate the culture base-material of morel, with implementing 3.
Above-mentioned culture base-material is utilized to carry out the method for morel cultivation, with embodiment 1.
Embodiment 9
Utilize cellulose ethanol waste residue to cultivate a culture base-material for morel, prepared by the following raw material of weight part: cellulose ethanol waste residue 48 weight part, cotton seed hulls 12 weight part, husk 15 weight part, wheat bran 12 weight part, Pericarppium arachidis hypogaeae 11 weight part, calcium superphosphate 1 weight part, lime 2 weight part.
The above-mentioned preparation method utilizing cellulose ethanol waste residue to cultivate the culture base-material of morel, with implementing 4.
Above-mentioned culture base-material is utilized to carry out the method for morel cultivation, with embodiment 1.
Below by compared with prior art, set forth the present invention further, it should be noted that, following testing data be all in northern environment cultivation survey data.Wherein, the said north is as the criterion with Geographic.
The culture base-material of morel cultivation conventional in prior art is utilized to be contrast, as a control group.Morel experiment in cultivation is carried out to embodiment 1 ~ 9 and control group.In test, embodiment group and control group adopt identical morel kind, and it is identical with the temperature in management of producing mushroom, humidity, illumination, ventilation management work and earthing condition that bacterium is sent out in inoculation, and observation fruiting time, fruiting phase and output are as shown in table 1.
The experimental result of table 1 morel fruiting time, fruiting phase and output
Test-results is as follows: after utilizing cellulose ethanol waste residue to substitute, different culture base-materials has certain difference in fruiting time of buddingging, stopping fruiting time and output, the fruiting time of buddingging of embodiment 1 ~ 9 gained morel all early than control group, and does sth. in advance 12 ~ 33 days; Stop the fruiting time to be then later than control group, namely the fruiting phase is long; Output aspect, every per mu yield of embodiment 1 ~ 9 gained morel is all higher than control group, and embodiment 2 gained morel output is the highest, higher than control group 39.02%.
In addition, the grow situation of the present invention to the cellulose ethanol waste residue cultivar culture material mycelia utilizing Different Weight percentage composition in culture base-material is added up, respectively the mycelial growth situation inoculating latter 5th day, the 10th day and the 15th day is observed, mycelia primary growth speed (the average daily speeds of growth after mycelia field planting in 5 d), the speed of growth in mid-term (the average daily speed of growth after mycelia field planting in 10-15 d) and late growing stage speed (mycelia purseful number of days) directly can be obtained by above-mentioned observation, as shown in table 2.
Wherein, the composition of formula culture base-material is culture base-material moiety of the present invention: cellulose ethanol waste residue, corn cob, cotton seed hulls, wheat bran, husk, lime and calcium superphosphate.In table, shown % represents: the weight of cellulose ethanol waste residue accounts for the per-cent of culture base-material gross weight.Each numerical value shown in table 2 is all the mean value of measurement 30 bags of growing states.Control group is the culture base-material composition of morel cultivation conventional in prior art.
Table 2 ethanol cellulose waste residue cultivation Morciiella Esculeuta Mycelia grows situation
Can be found out by table 2, the content that culture base-material adds cellulose ethanol waste residue is different, gained Morciiella Esculeuta Mycelia grows situation obvious difference, when the weight of cellulose ethanol waste residue accounts for 35% ~ 60% of culture base-material gross weight, the situation of growing of Morciiella Esculeuta Mycelia is all better than control group, especially when the weight of cellulose ethanol waste residue accounts for 50% of culture base-material gross weight, the situation of growing of Morciiella Esculeuta Mycelia is best.
Using cellulose ethanol waste residue as the culture base-material of major ingredient, low raw-material cost, making method are simple, mycelial growth is healthy and strong, not only save cost, and morel budding fruiting time advance, the fruiting phase extend, output gets a promotion.Cellulose ethanol waste residue is recycled, decreases the pollution of cellulose ethanol waste residue to environment, improve the biological transformation ratio of culturing raw material and the economic benefit of morel cultivation.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are realized or uses the present invention.
Claims (10)
1. utilize cellulose ethanol waste residue to cultivate the culture base-material of morel, it is characterized in that: described culture base-material is prepared by the raw material of following weight part: the auxiliary material of the cellulose ethanol waste residue of 35 ~ 60 weight parts, the plant organic matter of 35 ~ 55 weight parts, 3 ~ 5 weight parts.
2. utilize cellulose ethanol waste residue to cultivate the culture base-material of morel as claimed in claim 1, it is characterized in that: described plant organic matter is one or more the composition in cotton seed hulls, husk, Pericarppium arachidis hypogaeae, wheat bran, corn cob, agricultural crop straw, branch, leaf.
3. utilize cellulose ethanol waste residue to cultivate the culture base-material of morel as claimed in claim 2, it is characterized in that: described agricultural crop straw is
wheatstalk, rice straw,
cornstalk, potato class stalk,
cottonstalk,
rapestalk, peanut straw,
sugarcaneone or more compositions in stalk.
4. utilize cellulose ethanol waste residue to cultivate the culture base-material of morel as claimed in claim 1, it is characterized in that: described auxiliary material is one or more combinations in gypsum, lime, calcium superphosphate, potassium primary phosphate, magnesium sulfate, VITAMIN.
5. utilize cellulose ethanol waste residue to cultivate the culture base-material of morel as claimed in claim 1, it is characterized in that: described culture base-material is prepared by the following raw material of weight part: the cotton seed hulls of the cellulose ethanol waste residue of 35 ~ 60 weight parts, the corn cob of 2.5 ~ 7.5 weight parts, 12.5 ~ 17.5 weight parts, the wheat bran of 10 ~ 15 weight parts, the husk of 10 ~ 15 weight parts, the lime of 2 weight parts, the calcium superphosphate of 1 weight part.
6. utilize cellulose ethanol waste residue to cultivate a preparation method for the culture base-material of morel, it is characterized in that: comprise the following steps:
1) get the raw materials ready: each raw material preparing and take above-mentioned weight part respectively;
2) mix: mixed by the above-mentioned each raw material taken, and stir, add water the water content and pH value that regulate mixture, obtains wet feed;
3) pack: will through above-mentioned steps gained wet feed, adopt artificial or mechanical system to load in the packing bag making morel substratum, obtain pocket;
4) sterilizing: above-mentioned pocket is adopted normal-pressure sterilization, is rapidly heated to 100 ± 2 DEG C, maintains 20h.
7. utilize cellulose ethanol waste residue to cultivate the preparation method of culture base-material of morel as claimed in claim 6, it is characterized in that: described cellulose ethanol waste residue and cotton seed hulls are sprayed water the 10h that prewets before combination, described corn cob and husk are sprayed water the 5h that prewets before combination.
8. utilize cellulose ethanol waste residue to cultivate the preparation method of the culture base-material of morel as claimed in claim 6, it is characterized in that: in described mixing step, the water content control of described wet feed is at 60 ~ 65wt%, and pH value is 6.5 ~ 7.5.
9. utilize cellulose ethanol waste residue to cultivate the preparation method of the culture base-material of morel as claimed in claim 6, it is characterized in that: in described pouch step, in described each packing bag, fill siccative 0.5 ~ 0.6kg.
10. utilize cellulose ethanol waste residue to cultivate the preparation method of the culture base-material of morel as claimed in claim 6, it is characterized in that: described packing bag employing specification is the polypropylene plastics pocket of 17cm × 38cm × 0.05cm or 20cm × 33cm × 0.05cm.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105154342A (en) * | 2015-10-27 | 2015-12-16 | 宋瑞鹏 | Method for cultivating liquid-state morchella strain |
| CN106518358A (en) * | 2016-11-04 | 2017-03-22 | 颍上县唐垛湖现代农业科技有限公司 | Mineral matter-enriched efficient utilization-type golden mushroom cultivation material and preparation method thereof |
| CN106518360A (en) * | 2016-11-04 | 2017-03-22 | 颍上县唐垛湖现代农业科技有限公司 | Sustainably utilized efficient edible fungus culture medium and preparation method thereof |
| CN109168960A (en) * | 2018-10-31 | 2019-01-11 | 芒市喜丰农业发展有限公司 | A kind of hickory chick ecology expanding propagation method effectively improving yield |
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| CN1095885A (en) * | 1993-10-30 | 1994-12-07 | 朱斗锡 | The cultivation method of hickory chick |
| CN102161603A (en) * | 2010-12-28 | 2011-08-24 | 南阳天冠生物发酵有限公司 | Method for producing culture material of edible fungi by utilizing cellulosic ethanol factory waste material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1095885A (en) * | 1993-10-30 | 1994-12-07 | 朱斗锡 | The cultivation method of hickory chick |
| CN102161603A (en) * | 2010-12-28 | 2011-08-24 | 南阳天冠生物发酵有限公司 | Method for producing culture material of edible fungi by utilizing cellulosic ethanol factory waste material |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154342A (en) * | 2015-10-27 | 2015-12-16 | 宋瑞鹏 | Method for cultivating liquid-state morchella strain |
| CN106518358A (en) * | 2016-11-04 | 2017-03-22 | 颍上县唐垛湖现代农业科技有限公司 | Mineral matter-enriched efficient utilization-type golden mushroom cultivation material and preparation method thereof |
| CN106518360A (en) * | 2016-11-04 | 2017-03-22 | 颍上县唐垛湖现代农业科技有限公司 | Sustainably utilized efficient edible fungus culture medium and preparation method thereof |
| CN109168960A (en) * | 2018-10-31 | 2019-01-11 | 芒市喜丰农业发展有限公司 | A kind of hickory chick ecology expanding propagation method effectively improving yield |
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Inventor after: Liu Zijie Inventor after: Li Junjie Inventor after: Zeng Zhaoguo Inventor after: Chen Jinping Inventor after: Hua Dongbiao Inventor after: Guo Guizhen Inventor after: Zhou Xin Inventor after: Li Haoyan Inventor after: Yu Baofeng Inventor after: Lu Chunyan Inventor after: Du Minhua Inventor after: Ma Rubin Inventor after: Du Keheng Inventor after: Li Guoyun Inventor after: Bai Mingxia Inventor after: Deng Baohong Inventor before: Liu Zijie Inventor before: Hua Dongbiao Inventor before: Guo Guizhen Inventor before: Zhou Xin Inventor before: Li Haoyan Inventor before: Lu Chunyan Inventor before: Du Minhua Inventor before: Du Keheng Inventor before: Bai Mingxia Inventor before: Deng Baohong Inventor before: Li Junjie Inventor before: Zeng Zhaoguo Inventor before: Chen Jinping |
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Application publication date: 20151021 |