CN104981546B - 马铃薯原料的糖化方法和液体燃料的制造方法 - Google Patents
马铃薯原料的糖化方法和液体燃料的制造方法 Download PDFInfo
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- CN104981546B CN104981546B CN201380010643.8A CN201380010643A CN104981546B CN 104981546 B CN104981546 B CN 104981546B CN 201380010643 A CN201380010643 A CN 201380010643A CN 104981546 B CN104981546 B CN 104981546B
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- potato
- raw material
- enzyme
- acremonium
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Links
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 146
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 146
- 239000007788 liquid Substances 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 51
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 24
- 239000000446 fuel Substances 0.000 title abstract description 15
- 235000012015 potatoes Nutrition 0.000 title description 3
- 102000004190 Enzymes Human genes 0.000 claims abstract description 85
- 108090000790 Enzymes Proteins 0.000 claims abstract description 85
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 27
- 230000028327 secretion Effects 0.000 claims abstract description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 50
- 238000000855 fermentation Methods 0.000 claims description 40
- 230000004151 fermentation Effects 0.000 claims description 40
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 claims description 34
- 239000000835 fiber Substances 0.000 claims description 24
- 239000001963 growth medium Substances 0.000 claims description 22
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- 238000012545 processing Methods 0.000 claims description 13
- 241000726108 Blastocystis Species 0.000 claims description 7
- 241001215623 Talaromyces cellulolyticus Species 0.000 claims description 7
- 239000002609 medium Substances 0.000 claims description 4
- -1 lcohol Species 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
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- 239000002994 raw material Substances 0.000 abstract description 65
- 241000233866 Fungi Species 0.000 abstract description 45
- 210000000170 cell membrane Anatomy 0.000 abstract description 10
- 238000005054 agglomeration Methods 0.000 abstract description 7
- 229940088598 enzyme Drugs 0.000 description 81
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 34
- 229910052799 carbon Inorganic materials 0.000 description 34
- 239000000243 solution Substances 0.000 description 23
- 108010059892 Cellulase Proteins 0.000 description 22
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 19
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 19
- 229920002472 Starch Polymers 0.000 description 19
- 239000008103 glucose Substances 0.000 description 19
- 239000008107 starch Substances 0.000 description 19
- 235000019698 starch Nutrition 0.000 description 19
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 16
- 239000000203 mixture Substances 0.000 description 14
- 241000894006 Bacteria Species 0.000 description 13
- 230000000694 effects Effects 0.000 description 12
- 235000015097 nutrients Nutrition 0.000 description 11
- 239000001814 pectin Substances 0.000 description 11
- 229920001277 pectin Polymers 0.000 description 11
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- 238000000354 decomposition reaction Methods 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 108090000604 Hydrolases Proteins 0.000 description 7
- 102000004157 Hydrolases Human genes 0.000 description 7
- 239000002893 slag Substances 0.000 description 7
- 241000228150 Penicillium chrysogenum Species 0.000 description 6
- 238000004108 freeze drying Methods 0.000 description 6
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- 239000004382 Amylase Substances 0.000 description 4
- 102000013142 Amylases Human genes 0.000 description 4
- 108010065511 Amylases Proteins 0.000 description 4
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 4
- 235000019418 amylase Nutrition 0.000 description 4
- 239000012228 culture supernatant Substances 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 241000228143 Penicillium Species 0.000 description 3
- 240000005384 Rhizopus oryzae Species 0.000 description 3
- 235000013752 Rhizopus oryzae Nutrition 0.000 description 3
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- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 2
- 102000002659 Amyloid Precursor Protein Secretases Human genes 0.000 description 2
- 108010043324 Amyloid Precursor Protein Secretases Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 244000205754 Colocasia esculenta Species 0.000 description 2
- 235000006481 Colocasia esculenta Nutrition 0.000 description 2
- 241000235649 Kluyveromyces Species 0.000 description 2
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 241000235645 Pichia kudriavzevii Species 0.000 description 2
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- 241001123227 Saccharomyces pastorianus Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 241000499912 Trichoderma reesei Species 0.000 description 2
- 241000452385 Trichoderma reesei RUT C-30 Species 0.000 description 2
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- 229940031154 kluyveromyces marxianus Drugs 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- WDMUXYQIMRDWRC-UHFFFAOYSA-N 2-hydroxy-3,4-dinitrobenzoic acid Chemical compound OC(=O)C1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1O WDMUXYQIMRDWRC-UHFFFAOYSA-N 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- 241000235644 Issatchenkia Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 108010076039 Polyproteins Proteins 0.000 description 1
- 241000235346 Schizosaccharomyces Species 0.000 description 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 230000003625 amylolytic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
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- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
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- 230000002255 enzymatic effect Effects 0.000 description 1
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- 210000002683 foot Anatomy 0.000 description 1
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- 239000007791 liquid phase Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000001472 potassium tartrate Substances 0.000 description 1
- 235000011005 potassium tartrates Nutrition 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 150000003672 ureas Chemical class 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012-037404 | 2012-02-23 | ||
JP2012037404A JP5843264B2 (ja) | 2012-02-23 | 2012-02-23 | ジャガイモ原料の糖化方法及び液体燃料の製造方法 |
PCT/JP2013/052485 WO2013125336A1 (ja) | 2012-02-23 | 2013-02-04 | ジャガイモ原料の糖化方法及び液体燃料の製造方法 |
Publications (2)
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CN104981546A CN104981546A (zh) | 2015-10-14 |
CN104981546B true CN104981546B (zh) | 2018-02-13 |
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CN201380010643.8A Active CN104981546B (zh) | 2012-02-23 | 2013-02-04 | 马铃薯原料的糖化方法和液体燃料的制造方法 |
Country Status (3)
Country | Link |
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JP (1) | JP5843264B2 (ja) |
CN (1) | CN104981546B (ja) |
WO (1) | WO2013125336A1 (ja) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101633938A (zh) * | 2009-09-01 | 2010-01-27 | 山西省生物研究所 | 马铃薯废渣发酵燃料酒精的制备方法 |
Family Cites Families (4)
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JP4876247B2 (ja) * | 2006-04-19 | 2012-02-15 | 国立大学法人山口大学 | ペニシリウム属に属する新規微生物 |
CN101041849A (zh) * | 2007-04-26 | 2007-09-26 | 湖南南方马铃薯产业化工程技术有限公司 | 一种以马铃薯为原料生产燃料乙醇的方法 |
JP5339250B2 (ja) * | 2008-07-28 | 2013-11-13 | 独立行政法人産業技術総合研究所 | 酵素液の製造方法及び糖の製造方法 |
JP5629886B2 (ja) * | 2010-04-06 | 2014-11-26 | 学校法人東京農業大学 | 発酵袋を利用した発酵システム及びエタノール製造方法 |
-
2012
- 2012-02-23 JP JP2012037404A patent/JP5843264B2/ja active Active
-
2013
- 2013-02-04 WO PCT/JP2013/052485 patent/WO2013125336A1/ja active Application Filing
- 2013-02-04 CN CN201380010643.8A patent/CN104981546B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101633938A (zh) * | 2009-09-01 | 2010-01-27 | 山西省生物研究所 | 马铃薯废渣发酵燃料酒精的制备方法 |
Non-Patent Citations (2)
Title |
---|
Acremonium cellulolyticus纤维素酶在纤维素乙醇生产中的应用;方诩;《全国生物化工学术、技术交流会论文集》;20100312;摘要及第115页倒数第1段 * |
Practical Liquefaction of Potato Pulp and Sugar-beet Pulp by Commercial Enzymes;Miyaji等;《东京农大农学集报》;20070920;第52卷(第3期);摘要、第148页左栏倒数第1段、第149页右栏第1段及表2 * |
Also Published As
Publication number | Publication date |
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JP5843264B2 (ja) | 2016-01-13 |
CN104981546A (zh) | 2015-10-14 |
WO2013125336A1 (ja) | 2013-08-29 |
JP2013172645A (ja) | 2013-09-05 |
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