CN104981546B - The method for saccharifying of raw potatoes and the manufacture method of liquid fuel - Google Patents
The method for saccharifying of raw potatoes and the manufacture method of liquid fuel Download PDFInfo
- Publication number
- CN104981546B CN104981546B CN201380010643.8A CN201380010643A CN104981546B CN 104981546 B CN104981546 B CN 104981546B CN 201380010643 A CN201380010643 A CN 201380010643A CN 104981546 B CN104981546 B CN 104981546B
- Authority
- CN
- China
- Prior art keywords
- potato
- raw material
- enzyme
- acremonium
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 146
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 146
- 239000007788 liquid Substances 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 51
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 24
- 239000000446 fuel Substances 0.000 title abstract description 15
- 235000012015 potatoes Nutrition 0.000 title description 3
- 102000004190 Enzymes Human genes 0.000 claims abstract description 85
- 108090000790 Enzymes Proteins 0.000 claims abstract description 85
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 27
- 230000028327 secretion Effects 0.000 claims abstract description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 50
- 238000000855 fermentation Methods 0.000 claims description 40
- 230000004151 fermentation Effects 0.000 claims description 40
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 claims description 34
- 239000000835 fiber Substances 0.000 claims description 24
- 239000001963 growth medium Substances 0.000 claims description 22
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 19
- 244000005700 microbiome Species 0.000 claims description 16
- 238000012545 processing Methods 0.000 claims description 13
- 241000726108 Blastocystis Species 0.000 claims description 7
- 241001215623 Talaromyces cellulolyticus Species 0.000 claims description 7
- 239000002609 medium Substances 0.000 claims description 4
- -1 lcohol Species 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 230000005906 menstruation Effects 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 65
- 241000233866 Fungi Species 0.000 abstract description 45
- 210000000170 cell membrane Anatomy 0.000 abstract description 10
- 238000005054 agglomeration Methods 0.000 abstract description 7
- 229940088598 enzyme Drugs 0.000 description 81
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 34
- 229910052799 carbon Inorganic materials 0.000 description 34
- 239000000243 solution Substances 0.000 description 23
- 108010059892 Cellulase Proteins 0.000 description 22
- 229940106157 cellulase Drugs 0.000 description 22
- 239000001913 cellulose Substances 0.000 description 22
- 229920002678 cellulose Polymers 0.000 description 22
- 235000010980 cellulose Nutrition 0.000 description 21
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 19
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 19
- 229920002472 Starch Polymers 0.000 description 19
- 239000008103 glucose Substances 0.000 description 19
- 239000008107 starch Substances 0.000 description 19
- 235000019698 starch Nutrition 0.000 description 19
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 16
- 239000000203 mixture Substances 0.000 description 14
- 241000894006 Bacteria Species 0.000 description 13
- 230000000694 effects Effects 0.000 description 12
- 235000015097 nutrients Nutrition 0.000 description 11
- 239000001814 pectin Substances 0.000 description 11
- 229920001277 pectin Polymers 0.000 description 11
- 235000010987 pectin Nutrition 0.000 description 11
- 238000000354 decomposition reaction Methods 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 108090000604 Hydrolases Proteins 0.000 description 7
- 102000004157 Hydrolases Human genes 0.000 description 7
- 239000002893 slag Substances 0.000 description 7
- 241000228150 Penicillium chrysogenum Species 0.000 description 6
- 238000004108 freeze drying Methods 0.000 description 6
- 230000006698 induction Effects 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000004382 Amylase Substances 0.000 description 4
- 102000013142 Amylases Human genes 0.000 description 4
- 108010065511 Amylases Proteins 0.000 description 4
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 4
- 235000019418 amylase Nutrition 0.000 description 4
- 239000012228 culture supernatant Substances 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 241000228143 Penicillium Species 0.000 description 3
- 240000005384 Rhizopus oryzae Species 0.000 description 3
- 235000013752 Rhizopus oryzae Nutrition 0.000 description 3
- 230000001925 catabolic effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 238000005360 mashing Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 150000002772 monosaccharides Chemical class 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 2
- 102000002659 Amyloid Precursor Protein Secretases Human genes 0.000 description 2
- 108010043324 Amyloid Precursor Protein Secretases Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 244000205754 Colocasia esculenta Species 0.000 description 2
- 235000006481 Colocasia esculenta Nutrition 0.000 description 2
- 241000235649 Kluyveromyces Species 0.000 description 2
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 241000235645 Pichia kudriavzevii Species 0.000 description 2
- 241000235070 Saccharomyces Species 0.000 description 2
- 241000824156 Saccharomyces cerevisiae IR-2 Species 0.000 description 2
- 244000253911 Saccharomyces fragilis Species 0.000 description 2
- 235000018368 Saccharomyces fragilis Nutrition 0.000 description 2
- 241001123227 Saccharomyces pastorianus Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 241000499912 Trichoderma reesei Species 0.000 description 2
- 241000452385 Trichoderma reesei RUT C-30 Species 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000002478 diastatic effect Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 229940031154 kluyveromyces marxianus Drugs 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- WDMUXYQIMRDWRC-UHFFFAOYSA-N 2-hydroxy-3,4-dinitrobenzoic acid Chemical compound OC(=O)C1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1O WDMUXYQIMRDWRC-UHFFFAOYSA-N 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- 241000235644 Issatchenkia Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 108010076039 Polyproteins Proteins 0.000 description 1
- 241000235346 Schizosaccharomyces Species 0.000 description 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 230000003625 amylolytic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008618 cell wall macromolecule catabolic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000002683 foot Anatomy 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 150000002303 glucose derivatives Chemical class 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000012770 industrial material Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000001472 potassium tartrate Substances 0.000 description 1
- 235000011005 potassium tartrates Nutrition 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 150000003672 ureas Chemical class 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The method that will be effectively saccharified from the raw material of potato tubers is provided and utilizes the manufacture method of the liquid fuel for the raw material for deriving from potato tubers.Especially provide the manufacture method of saccharide, it is characterised in that will be handled from the raw material of potato tubers with the secretion enzyme liquid of the cell membrane high de-agglomeration filamentous fungi containing hydrolase.
Description
Technical field
It the present invention relates to the use of the manufacturer of the method for saccharifying of the raw potatoes of enzyme and the liquid fuel based on this method
Method.
Background technology
The liquid fuels such as the ethanol by biomass manufacture help to cut down greenhouse effect gas due to that can substitute oil
Discharge rate, thus its production and using being developed in the world.
Using starch as principal component beyond the stem tuber moisture removal of potato, by starch starch enzymatic conversion, and it is allowed to ferment
It is to be relatively easy to manufacture the liquid fuels such as ethanol.But potato tubers also contains cellulose, pectin etc. in addition to starch
Composition, it is difficult to decompose amylase used during these compositions only decompose by starch.Particularly extracted from potato tubers
Residue after starch（Liquid is referred to as potato residues or farina extraction residue）In, the content ratio phase of cellulose, pectin etc.
Increase over the ground, using them as the cell membrane of composition in the utilization of starch that remains become difficult, this, which turns into, limits above-mentioned residue
Utilization the main reason for.Recorded in non-patent literature 1 secretion the starch in potato residues is decomposed from the enzyme of Rhizopus oryzae and
Lactic acid is generated, but the cell wall breakdown ability of Rhizopus oryzae is low（The table 2 of non-patent literature 1）And lactic acid generation can cause sugar
Yield reduces, thus the method for saccharifying of the starch inside the cell membrane of use Rhizopus oryzae still has the leeway of improvement.In addition, should
A variety of enzymes are needed to use in the decomposition of potato residues, it was reported that preferably it is expected to use 3 kinds of enzymes（Non-patent literature 2）.But
The main reason for use of a variety of enzymes is cost rise, thus need more simple and inexpensive saccharification technology.
Prior art literature
Non-patent literature
Non-patent literature 1：Oda Y., et al., Curr Microbiol., (2002) 45(1): p.1-4
Non-patent literature 2：Miyaji et al., J. Agric. Sci., Tokyo Univ. Agric., 52(3)
147-150 (2007)。
The content of the invention
Problems to be solved by the invention
The problem of the present invention be to provide the method that will be effectively saccharified from the raw material of potato tubers and using come
Come from the manufacture method of the liquid fuel of the raw material of potato tubers.
For solving the method for problem
The present inventor furthers investigate repeatedly in order to solve above-mentioned problem, as a result finds, filamentous fungi can be produced to source
It is raw material in taro class, especially effective as the efficient saccharification of the raw material from potato tubers of representative using potato residues
Enzyme group, so as to complete the present invention.
That is, the present invention is comprising following.
[1] manufacture method of saccharide, it is characterised in that by from the raw material of potato tubers with containing hydrolase
The secretion enzyme liquid of cell membrane high de-agglomeration filamentous fungi handled.
The method of [2] above-mentioned [1], wherein, the raw material from potato tubers is potato residues.
[3] method described in above-mentioned [1] or [2], wherein, cell membrane high de-agglomeration filamentous fungi is that acremonium belongs to thread
Fungi or Penicillium filamentous fungi.
[4] method of any one of above-mentioned [1]~[3], wherein, cell membrane high de-agglomeration filamentous fungi is Xie Xianweiding
Spore is mould（Acremonium cellulolyticus）.
[5] method of any one of above-mentioned [1]~[3], wherein, cell membrane high de-agglomeration filamentous fungi is Xie Xianweiding
The mould TN strains of spore（Deposit number FERM BP-11452）Or 101300 plants of Penicillium notatum NBRC.
[6] method of any one of above-mentioned [1]~[5], wherein, with the training containing the raw material from potato tubers
The generation of the foregoing filamentous fungi of base culture and induced hydrolysis enzyme is supported, thus prepares foregoing secretion enzyme liquid, and use it for deriving from
The processing of the raw material of potato tubers.
[7] manufacture method of alcohol, it is characterised in that carried out to saccharide obtained by the method by above-mentioned [1]~[6]
Alcohol fermentation.
The method of [8] above-mentioned [7], wherein, carry out alcohol fermentation using Blastocystis yeast.
[9] method of above-mentioned [7] or [8], it carries out alcohol fermentation using Blastocystis yeast to foregoing saccharide
The manufacture method of ethanol.
[10] method of above-mentioned [7]~[9], wherein, with the training of the saccharide containing the raw material from potato tubers
Support before base is carried out to fermentative microorganism and cultivate, and alcohol fermentation is carried out with it.
This specification includes the content of the Japanese Patent Application 2012-037404 as the application priority request basis.
Invention effect
The method according to the invention, efficiently it can will hydrolyze and be saccharified from the raw material of potato tubers.
Brief description of the drawings
[Fig. 1] Fig. 1 is to show the figure using the sugared receipts amount obtained by the saccharification of the potato residues of commercially available cellulase.A tables
Show that glucose, B represent the receipts amount of galactolipin（g/L）.
[Fig. 2] Fig. 2 is to show to utilize the solution fiber acremonium using cellulose powder or potato residues as carbon source
（Acremonium cellulolyticus）Cellulase yield figure.
[Fig. 3] Fig. 3 is to show the figure that cellulose powder addition influences caused by being produced on cellulase." cellulose powder "
The result of the culture using the culture medium containing 5% cellulose powder is represented, " only potato residues ", which represent to use, contains 5% potato
The culture medium of slag（Cellulose powder addition 0%）Culture result, " potato residues+0.5% ", " potato residues+1.5% ",
" potato residues+5% " represent to use respectively to contain 5% potato residues+cellulose powder addition：0.5%th, 1.5%, 5% culture
The result of the culture of base.
[Fig. 4] Fig. 4 has been shown with by solution fiber acremonium（Acremonium cellulolyticus）Caused enzyme
Saccharification sugared receipts amount figure.A：Glucose amount（g/L）、B：Gala sugar amount（g/L）.In each figure, from being acremonium fiber from left to right
Plain enzyme preparation（Marketed cellulose enzyme preparation）, cellulose powder induction enzyme liquid（Induced and produced using cellulose powder as carbon source
Enzyme liquid）, potato residues induction enzyme liquid（The enzyme liquid caused by induction using potato residues as carbon source）.White bars represent 24 hours
Saccharification result, black bar represent 48 hours saccharification result.
[Fig. 5] Fig. 5 is the sugar for being shown with the potato residues of enzyme liquid obtained by 3 kinds of filamentous fungis of potato residues culture
The figure of the sugared receipts amount of change.A：Glucose amount（g/L）、B：Gala sugar amount（g/L）.In figure, rhombus represents acremonium category bacterium（Solve fiber
Acremonium TN strains）Result, square represent trichoderma bacterium（Trichoderma reesei RUT C-30 strains）Result, triangular representation it is blue or green
Mould category bacterium（101300 plants of Penicillium notatum NBRC）Result.
[Fig. 6] Fig. 6 is to be shown with the potato saccharified liquid figure horizontal as the propagation of the yeast of culture nutrient source.Each examination
What percentage described in sample name represented each composition in culture medium contains concentration.
[Fig. 7] Fig. 7 is shown with using potato residues and cellulose as enzyme liquid obtained by carbon source culture filamentous fungi, to horse
Bell potato slag carries out the figure of the concentration of ethanol obtained by diastatic fermentation.
Embodiment
Hereinafter, the present invention is described in detail.
In the method for the present invention, raw material is used as using the raw material from potato tubers.From potato tubers
Raw material refers to the stem tuber or its processed material of potato, and is processed into the state suitable for ferment treatment.From potato tubers
Raw material does not limit, for example, in addition to the form of potato tubers in itself, can also be section, disorderly chops thing, stripping and slicing, smashs to pieces
Thing, fragment, crushed material crush the arbitrary forms such as suspended things, can remove or not remove peeling, bud, preferably as far as possible
Keep potato tubers contains composition（Particularly starch, cellulose etc.）State.In addition, the original from potato tubers
Material can be residue caused by processing potato stem tuber, for example, particularly preferably potato residues.Potato residues mean with Ma Ling
The product that residue after being separated when potato wedge stem carries out the extraction of farina for raw material as farina remains.Ma Ling
Potato slag is also referred to as potato extraction residue.Raw material from potato tubers is stem tuber or its processing of above-mentioned potato
Thing and the state suitable for ferment treatment is processed into, for example, it may be by residue caused by processing potato stem tuber and other lifes
The material that raw material of substance is mixed together.In addition, from potato tubers raw material can be raw state or
Heated state.Raw material from potato tubers can be chilled state or through dry state, also
It can be freeze-drying thing.Raw material from potato tubers can carry out sterilization processing.As one, for from horse
The raw material of bell potato wedge stem, 121 DEG C, 10~30 minutes or so can be carried out used by the sterilization processing in usual microculture
Autoclave process.Thereby, the living contaminants in diastatic fermentation step of the raw material through sterilization and after can preventing, also simultaneously
The effect of the decomposability of raising raw material can be expected, thus it is useful processing.
In the present invention, potato tubers refers to generally edible potato underground stem portion.The horse used in the present invention
Bell potato can be any kind or its mutation or wild species.
Filamentous fungi used is the fungi with the ability for producing a variety of biomass decomposition enzymes of secretion in the present invention, excellent
Elect as its secretase it is at least one kind of possess the main composition of potato tubers i.e. starch, cellulose, pectin etc. are hydrolyzed to
Form the fungi of the ability of sugar.The filamentous fungi used in the present invention is preferably cell membrane high de-agglomeration filamentous fungi.The present invention
In, cell membrane high de-agglomeration filamentous fungi is to refer to the composition that high-efficiency decomposition of cellulose, pectin etc. form cell membrane, and can be incited somebody to action
Containing these cell wall constituents for example from the raw material of potato tubers（Potato residues etc.）Decompose to the thread of liquid
Fungi.For example, belonging to can be listed as used in the present invention as the acremonium category of phorozoon or the filamentous fungi of Penicillium
Preferred filamentous fungi.As the filamentous fungi used in the present invention, preference can enumerate solution fiber acremonium（Acremonium
cellulolyticus）.The particularly preferred example of the filamentous fungi used in the present invention is solution fiber acremonium
（Acremonium cellulolyticus）TN strains and mould（Penicillium）101300 plants of bacterium NBRC.
Various filamentous fungis can be obtained by commercially available product or culture presevation or the preservation mechanism based on budapest treaty
.
Solution fiber acremonium TN strains are on January 12nd, 2012, based on budapest treaty, with deposit number FERM
BP-11452, international accession is in independent administrative corporation's product assessment technique fundamental mechanism Patent Organism collection（NITE-
IPOD；The center of a kind of ground of postcode 305-8566 this country Ci Cheng Ken つ く ば Shi 1 fourth mesh of East 1 the 6th）.
101300 plants of Penicillium notatum NBRC is recorded in independent administrative corporation's product assessment technique fundamental mechanism（NITE；Qian Leaf
Ken Mu Geng Jinshi City か ず さ Sickle foots 2-5-8）NBRC（NITE Biological Resource Centers)（Japan）Catalogue ［ NBRC
Catalogue of Biological Resources, Microorganisms, Microorganism-Related DNA
Resources, Human-Related DNAResources, Second Edition (2010) ］ in, can be with numbering
101300 are obtained by NBRC.
In the method for the present invention, in the processing from the raw material of potato tubers, produced using foregoing filamentous fungi
The secretion enzyme liquid containing hydrolase.In the present invention, the secretion enzyme liquid of filamentous fungi refers to produce by cultivating filamentous fungi,
Liquid containing secretion to the secretase group in culture medium（Enzyme liquid）.The secretion enzyme liquid of filamentous fungi contain it is one or more,
It is preferred that the enzyme of the ability of a variety of polysaccharides with contained by the potatos such as starch-splitting, cellulose, pectin（Hydrolase）, at least
Contain cellulase.The secretion enzyme liquid of the filamentous fungi of the present invention typically contains cellulase（Cellulolytic enzyme）, pectin
Enzyme（Pectin hydrolase）And amylase（Amylolytic enzyme）.The secretion enzyme liquid of the filamentous fungi of the present invention further preferably further contains
There is galactan catabolic enzyme.It should illustrate, the secretion enzyme liquid containing a variety of enzymes is expressed as enzyme system sometimes in this specification.These
Enzyme is that filamentous fungi produces and secreted after manufacture to extracellular in the cell.The secretion enzyme liquid can be filamentous fungi training
Support thing or culture supernatant.The secretion enzyme liquid of foregoing filamentous fungi can be by cultivating more than one in the presence of carbon source（It is preferred that 1
Kind）Filamentous fungi and the production of induced hydrolysis enzyme, be allowed to secrete the enzyme in its nutrient solution to prepare.Cultivate filamentous fungi
Culture medium can add carbon source etc. to prepare in any culture medium cultivated suitable for filamentous fungi.It is thread true as cultivating
The carbon source in culture medium needed for bacterium, arbitrary organic matter can be used, uses the raw material from potato tubers（Such as horse
Bell potato slag）Or its enzyme analyte（Such as saccharide）As carbon source in hydrolases such as cellulase induction, pectase and amylase
Generation on more preferably.In the situation of the latter, compared with using the situation of other carbon sources, it can produce to special in potato tubers
Not a large amount of existing compositions（Starch, cellulose, pectin etc.）The excellent enzyme of decomposability.Therefore, the culture of filamentous fungi is cultivated
Base preferably comprises at least one kind of in starch, cellulose and pectin（It is it is preferred that whole）As carbon source.Cultivate the culture medium of filamentous fungi
In, except the raw material from potato tubers（Such as potato residues）Outside, further preferably containing its enzyme analyte（Such as it is saccharified
Thing）Or the carbon source beyond the raw material of potato tubers（For example, the refined cellulose such as cellulose powder or purified starch
Deng）.Carbon source beyond the raw material of potato tubers can with such as more than 0.5%, preferably more than 1.5%, it is further excellent
More than 5%, such as 0.5~30% concentration is selected to make an addition in the raw material of potato tubers.Except from potato ball
Other carbon sources are also added to culture medium outside the raw material of stem, it is possible thereby to effectively further cellulase induction, pectase
With the generation of the hydrolase such as amylase.
The secretion enzyme liquid of filamentous fungi can be from the nutrient solution of this kind of filamentous fungi（Such as in the form of culture supernatant）Enter
Row collection, separation are refined to use, and can also be directly used in nutrient solution without gathering, separate or refining and be derived from
The processing of the raw material of potato tubers.As the secretion enzyme liquid of filamentous fungi, industrial commercial articles can be used（Example
Such as, acremonium cellulase（Meiji Seika Off ァ Le マ Co. Ltd. systems））.
, can be by the raw material from potato tubers or containing from potato tubers in the method for the present invention
Raw material solution or fluid nutrient medium in, add the secretion enzyme liquid of filamentous fungi and be allowed to reaction and to from potato ball
The raw material of stem carries out ferment treatment.
Or in method of the invention, can also be in the raw material from potato tubers or containing from potato
In the fluid nutrient medium of the raw material of stem tuber, the generation of filamentous fungi of the invention and the hydrolase such as cellulase induction is cultivated, and
It is allowed to secretion and secretion enzyme liquid is formed into culture medium, further adds the raw material from potato tubers thereto and be allowed to
Reaction, ferment treatment thus is carried out to the raw material from potato tubers.
Carried out under optimum temperature of the ferment treatment preferably in each enzyme, pH, for example, in the feelings of enzyme system caused by solution fiber acremonium
In shape, it is proper that 40~60 DEG C, pH4~6 or so, preferably 45~55 DEG C, pH4.5~5.5 are lower carries out.
Ferment treatment preferably uses with more than 5%, preferably more than 7%, more preferably more than 10%, it is further preferred more than 20%, also
It is preferred that more than 30%, such as more than 50% and less than 100% concentration, such as 5~50% concentration contain from potato tubers
Raw material（Preferably potato residues）Solution or culture medium carry out.
By enzyme reaction as described above, the polysaccharide of potato tubers is formed（Cellulose, starch, pectin etc.）It is hydrolyzed
And generate monosaccharide or oligosaccharide kind（For example, glucose, galactolipin, maltose, cellobiose etc.）（Saccharification reaction）.The present invention
The method that the raw material manufacture saccharide that this origin comes from potato tubers is also provided.In addition, the present invention is also provided using this
The method for saccharifying of the raw material from potato tubers of saccharification reaction.
In this way, by make from potato tubers it is raw saccharified obtained by saccharide（Typically saccharified liquid）Hair
Ferment, various utilities can be changed into.Especially, by alcohol fermentation, can be given birth to by the monosaccharide in saccharide or oligosaccharide kind
Produce alcohol.The alcohol of gained may be used as such as liquid fuel or industrial materials.
Alcohol fermentation can use the fermentative microorganism for carrying out alcohol fermentation, such as yeast, to carry out.Yeast is simultaneously not limited, can
Enumerate for example：Saccharomyces cerevisiae（Saccharomyces cerevisiae）, saccharomyces pastorianus（Saccharomyces
pastorianus）Deng Blastocystis（Saccharomyces）Bacterium, schizosaccharomyces pombe（Saccharomyces pombe)）Deng
Schizosccharomyces（Schizosaccharomyces）Bacterium, kluyveromyces marxianus（Kluyveromyces marxianus）
Deng Kluyveromyces（Kluyveromyces）Bacterium, Issatchenkia orientalis（Issatchenkia orientalis）Deng her Sa ferment
Mother's category（Issatchenkia）Bacterium etc..
For example, by using the Blastocystis yeast with ethanol fermentation ability as yeast, can be to above-mentioned saccharide
Carry out alcohol fermentation.Particularly preferred saccharomyces cerevisiae, is cultivated by being added into saccharide, can be by monose such as glucose
Class produces（Manufacture）Ethanol.The ethanol can use as the liquid fuel for being capable of replacing gasoline.By the fermentation using saccharomyces cerevisiae
Experiment shows, the inhibition to yeast is not observed from the saccharide of the raw material of potato tubers, may be used as good
Good fermentation raw material.In the present invention, " fermentative microorganism " refers to the microorganism with the ability fermented.
Above-mentioned fermentation is not limited to the alcohol fermentation using saccharomyces cerevisiae generation ethanol, also corresponds to the micro- life of fermentation used
Thing and manufacture other alcohol such as butanol.Such various alcohol can be used for liquid fuel etc..
By to saccharide obtained by raw saccharified from potato tubers is fermented, can also produce alcohol with
Outer material, for example, aliphatic acid etc. can be as the utility of liquid fuel component.The fermentation is micro- by using corresponding fermentation
Biology is realized.
Fermentative microorganism preferably carries out propagation and is prepared as a certain amount of step before the fermentation for saccharide（Generally
Referred to as preceding culture）.Process of the present invention it is preferred the culture medium pair with the saccharide containing the raw material from potato tubers
Fermentative microorganism is cultivated before carrying out, and carries out the fermentation of saccharide using it（It is preferred that alcohol fermentation）.Fermentation can be micro- in the fermentation
Carry out under the usual fermentation condition of biology, for example, can carry out under anaerobic, can also carry out under aerobic conditions.Wine brewing
In the situation of yeast, preferably carried out under the fermentation condition of anaerobism.
In the preceding culture of the present invention, the culture medium of the saccharide containing the raw material from potato tubers, for example, can be with
The saccharified liquid for being derived from the raw material of potato tubers in itself, can also add the saccharide of the raw material from potato tubers
Enter in other culture mediums such as culture medium to prepare.By using the saccharide of the raw material from potato tubers, with making
Compared with the situation that conventionally used nutrient source is blackstrap, the better propagation of microorganism can be obtained.In this way, pass through by
The microorganism that preceding culture has been carried out by the use of the saccharide of the raw material from potato tubers is used to be used as the saccharide formally cultivated
Fermentation in, alcohol fermentation can particularly well be carried out with efficiency.It should illustrate, with the saccharification of the raw material from potato tubers
Thing carried out preceding culture microorganism cannot be only used for from potato tubers raw material saccharide fermentation, can also be extensive
Ground is used in the fermentation using other various raw materials.
In the past, for the raw material from potato tubers, particularly potato residues etc., in the absence of can be comprehensive by its composition
Close property efficient-decomposition saccharification technology, turn into its obstacle utilized.In the method for the present invention, by using from thread
The enzyme system of fungi, contained cellulose in the raw material of potato tubers, starch, pectin etc. can be decomposed well with efficiency
Main component, as a result, it is possible to which efficiency obtains by fermentation to be changed into well the monosaccharide of liquid fuel.Pass through this
The method of invention, the comprehensive decomposition for being difficult to come from the raw material of potato tubers in the past become able to easily carry out, and lead to
Cross and it is fermented, the manufacture using alcohol as the liquid fuel of representative can be carried out, thus can provide conventional availability low life
Effective Application way of raw material of substance.
Whole publications, the patents and patent applicationss of issuing quoted in this specification are by reference to being fully incorporated this explanation
In book.
Embodiment
Hereinafter, enumerate embodiment to illustrate the present invention in more detail, but the present invention is not limited by them.
［ embodiment 1 ］
（1）Farina squeezes the analysis of slag basis
Obtain the potato residues gathered in the starch plants of Heilongjiang Province of China Beidahuang Potato Industry Group Co., Ltd.
（Farina squeezes slag）Freeze-drying thing, for aftermentioned mashing test.
Therefore, analyzed first by the composition composition of potato residues of the well-established law to using.The results are shown in table 1.
[table 1]
It should illustrate, the bottom of table 1 shows that the monose after the polysaccharide in above-mentioned potato residues is decomposed completely forms.The list
Sugar composition be potato residues are decomposed completely with sulfuric acid obtained by measured value.Glucose is substantially point by starch and cellulose
Solution generation, thus the total of the amount of starch and cellulose and the glucose content in monose composition are basically identical.
（2）Use the saccharification of enzyme
Above-mentioned（1）Freeze-drying potato residues（Sample）Middle addition water, it is 10%, 20%, 30% to make concentration（Each w/v）.
Wherein, with 10 Filter Paper Unit（FPU；Filter paper degrading activity）The ratio of/g substrates adds marketed cellulose enzyme system
Agent acremonium cellulase（Meiji Seika Off ァ Le マ Co. Ltd. systems；Solve cellulase caused by fiber acremonium）,
In 0.05M citrate buffer solution, carry out reacting for 24 hours or 48 hours at 50 DEG C, determine glucose, the galactolipin of generation
Amount.The measure of glucose and galactolipin is using the light splitting society of Japan for being provided with Aminex HPX-87H (BioRad societies) post
Highly effective liquid phase chromatographic system processed（Detector：Differential refractometer 2031Plus）, quantified on the basis of respective standard items.Its
As a result it is shown in Fig. 1.As shown in figure 1, the growing amount of glucose and galactolipin depends on the concentration of sample used and increased.Pass through enzyme
Processing, originally the liquid for the decomposition of gelatinous potato residues, generated the monose such as glucose, galactolipin.
On the other hand, the use of identical marketed cellulose enzyme preparation is Accellerase 1000（Genencore societies system；
From trichoderma reesei）, similarly carry out pair（1）Potato residues carry out ferment treatment experiment, even as a result 10% concentration,
Residue also not liquid, is not observed decomposition.
The result shows that cellulase caused by acremonium category bacterium is very suitable for from taro class, particularly deriving from horse
The saccharification of the raw material of bell potato.
［ embodiment 2 ］
（1）Produced using the enzyme of potato residues
It is carbon source to cellulase production fungi degradation fiber acremonium TN strains using potato residues（Deposit number：FERM BP-
11452）Cultivated, be allowed to produce enzyme.In addition, as control, also carry out will typically serve as the carbon source of cellulase production
The culture that cellulose powder substitutes potato residues and used as carbon source.
The composition of culture medium used is as described below in culture：24 g/L KH2PO4、1 g/L Tween 80、5 g/L
(NH4)2SO4、1.2 g/L MgSO4・7H2O、0.01 g/L ZnSO4・7H2O、0.01 g/L MnSO4・6H2O、0.01 g/L
CuSO47H2O, 4 g/L ureas, 4.7 g/L potassium tartrates monohydrates and the cellulose powder as carbon source（Solka Floc
（Registration mark）；Control）Or freeze-drying potato residues（5%、7%、10%）.
Culture is carried out under 30 DEG C of reaction temperature, mixing speed 200rpm.Cultivated before being carried out using cellulose powder as carbon source,
In the past after culture has started 72 hours, preceding nutrient solution is added in culture medium in a manner of reaching 5%, formally cultivated.
Gather the culture formally cultivated started 5 days after, the culture medium after 7 days, after 14 days, determine and secrete into culture medium
Enzyme-cellulose enzyme activity.The measure of cellulase activity is with filter paper（Whatman No.1）For substrate, in 50 mM lemons
Lemon acid buffer（pH 5.0）In reacted 60 minutes at 50 DEG C, pass through dinitrosalicylic acid system and the amount of the reduced sugar of generation carried out
Colorimetric, quantify, and activity is represented with FPU（Fig. 2）.
Although as shown in Fig. 2 low as the concentration of carbon source phase specific activity with using cellulose powder, by with potato residues
The enzyme with cellulase activity can be produced for the culture of carbon source.
And then with freeze-drying potato residues（5%）The material conduct of 0.5%, 1.5% or 5% cellulose powder of middle addition
Carbon source, carry out solving fiber acremonium in the same manner as described above（A. cellulolyticus）The culture of TN strains, it is allowed to produce enzyme.It is right
Culture medium after culture, determines cellulase activity in the same manner as described above（FPU activity）, results verification to in potato residues
Cellulose addition corresponding to FPU activity raising（Fig. 3）.
（2）Utilize the saccharification using potato residues as the potato residues of enzyme caused by carbon source
By the above-mentioned of the present embodiment（1）In using potato residues as culture obtained by carbon source culture solution fiber acremonium TN strains
Culture supernatant collection be used as enzyme liquid, use the pipe with milipore filter（VIVASPIN 6, Sartorius societies）Ultrafiltration is carried out to it
And concentration.It is respectively added to 30% with 5 FPU/g ratio（w/v）In potato residues, with embodiment 1-（2）Carry out in the same manner
The saccharification of 24 hours or 48 hours.
In the same manner, by the above-mentioned of the present embodiment（1）In using cellulose powder as carbon source culture solution fiber acremonium TN strains and
The culture supernatant collection of the culture obtained is used as enzyme liquid, and mashing test is carried out under reaction condition same as described above.In addition, make
For control, the mashing test of the marketed cellulose enzyme preparation acremonium cellulase used in embodiment 1 has been used also identical
Implement under reaction condition.
Then, for gained saccharified liquid, the amount of glucose and galactolipin is determined respectively same as Example 1ly.
Result above is shown in Fig. 4.As shown in figure 4, use is produced using potato residues as carbon source by solution fiber acremonium TN strains
Enzyme liquid, glucose and galactolipin can be produced by potato residues.In addition, use is using cellulose powder as enzyme liquid caused by carbon source
Or the situation of marketed cellulose enzyme preparation acremonium cellulase can also produce glucose and galactolipin by potato residues.Especially
It is use using potato residues during enzyme liquid, can be obtained caused by carbon source higher than use using cellulose powder as enzyme caused by carbon source
Sugared receipts amount when liquid or marketed cellulose enzyme preparation acremonium cellulase.For galactolipin, and using cellulose powder as carbon source
Caused enzyme liquid is compared, and significantly higher receipts amount can be obtained as the enzyme liquid that carbon source obtains using potato residues.It is thus regarded that with horse
When bell potato slag is carbon source, the generation of galactan catabolic enzyme is induced, and contains galactan catabolic enzyme in gained secretion enzyme liquid.
［ embodiment 3 ］
Solution fiber acremonium TN strains are substituted, by 101300 plants of filamentous fungi Penicillium notatum NBRC（Independent administrative corporation's product
Assessment technique fundamental mechanism（NITE）NBRC catalogue NBRC Catalogue of Biological Resources,
Microorganisms, Microorganism-Related DNA Resources, Human-Related DNA
Resources, Second Edition (2010) ］ in, obtained by NBRC）, trichoderma reesei RUT C-30 strains（the
American Type Culture Collection （ATCC）no.56765））Under the same conditions as in practical example 2, use
Potato residues are cultivated as carbon source, and enzyme liquid obtained by use makes potato residues be saccharified.For saccharified liquid, glucose is determined
Fig. 5 is shown in the result of the amount of galactolipin.
Its result shows that 3 kinds of filamentous fungis produce glucose and galactolipin.As shown in figure 5, using from solution fiber
Glucose amount highest during the enzyme liquid of acremonium, in saccharification particularly preferably.But for the enzyme liquid from Penicillium notatum, obtain
From about 75% glucose amount of the enzyme liquid of solution fiber acremonium, and for gala sugar amount, obtain than solution fiber acremonium
Higher receipts amount, therefore show that the bacterium is also preferred to being saccharified.The generation of saccharification enzyme of these bacterium for potato residues especially has
With.
For from as cellulase producing bacteria and industrially for the enzyme liquid of commonly used trichoderma reesei,
Residue liquid, saccharification start to need more than 40 hours, and it reacts relatively solution fiber acremonium TN strains and Penicillium notatum NBRC
101300 plants slow.
［ embodiment 4 ］
（1）Utilize the preceding culture of the fermentative microorganism of potato saccharified liquid
For microorganism used in the fermentation for making saccharified liquid increase to it is a certain amount of before cultivate in whether can be with
Tested using potato saccharified liquid as nutrient source.
By yeast Saccharomyces cerevisiae IR-2 strains（On April 8th, 1985, in independent administrative corporation's system
Judge valency technical foundation mechanism Patent Organism collection（1 kind of postcode 305-8566 this country 1 fourth mesh of Ci Cheng Ken つ く ば Shi East
The center of ground 1 the 6th）Preservation.Deposit number FERM BP-754）With YPD culture mediums（1% yeast extract, 1% polyprotein peptone,
2% glucose）A Dinner is cultivated, and with OD600Value（Optical density：The index of somatic cells number is represented using absorbance）Reach 0.1
Mode makes an addition to culture medium, and culture experiment is carried out under 30 DEG C, the rpm of mixing speed 120.
Culture medium be based on YPD culture mediums, using by potato residues in embodiment 1-（2）Middle commercially available enzyme acremonium
Saccharified liquid obtained by cellulase processing（Potato saccharified liquid）And prepared by the blackstrap of the sugar beet obtained in China.
Control medium is used as using YPD culture mediums.Incubation time is set to 6 hours, 18 hours or 24 hours.
Generally, in the culture of yeast, blackstrap is used as preferable nutrient source, but is saccharified by using potato
Liquid, obtain the proliferative amount of the yeast higher than blackstrap（Fig. 6）.
The result shows, serves not only as carbon source from the saccharide of the raw material of potato tubers, and be used as and include nitrogen
Or the comprehensive nutrient source of other inorganic constituents is also excellent.
（2）Alcohol fermentation is tested
Embodiment 2-（1）In, with freeze-drying potato residues（5%）0.5%, 1.5% or 5% cellulose powder of middle addition
Material as in enzyme liquid obtained by carbon source culture solution fiber acremonium TN strains, add yeast Saccharomyces
Cerevisiae IR-2 strains are fermented.In the fermentation, with OD600Yeast is added to foregoing saccharification by the mode that value reaches 0.1
After in liquid, carry out cultivating for 24 hours under 30 DEG C, the rpm of mixing speed 120.For the supernatant after gained culture, measure ethanol is received
Amount（Fig. 7）.The measure of ethanol is using the Japan's light splitting efficient liquid of society's system for being provided with Aminex HPX-87H (BioRad societies) post
Phase chromatographic system（Detector：Differential refractometer 2031Plus）Come carry out.
As a result the glucose in saccharified liquid is promptly consumed and is changed into ethanol, and its receipts amount reaches relative to theoretical value
97%（Fig. 7, " only potato residues "）.It is therefore shown that without the material for causing fermentation to hinder in saccharified liquid obtained by this method.
Industrial applicability
The method of the present invention can be by carrying out enzyme decomposition and to realizing efficient profit from the raw material of potato tubers
With, and can be used for handling it when it is discarded object.And then by the sugar obtained by the saccharification is fermented and
Changed, the manufacture of novel liquid fuel can also be implemented.Utilize the decomposition sugar of the raw material from potato of the present invention
Change technology can be widely used in effective utilization of its raw material, its its processing when being discarded object.It is sugared as obtained by the technology in addition
Compound is useful for efficiently manufacturing liquid fuel.
Claims (7)
1. the manufacture method of saccharide, it is characterised in that by solution fiber acremonium of the potato residues containing hydrolase
（Acremonium cellulolyticus）Secretion enzyme liquid individually handled, solution fiber acremonium be that deposit number is
FERM BP-11452 solution fiber acremonium TN strains.
2. the method described in claim 1, wherein, induce water with the medium culture solution fiber acremonium containing potato residues
The generation of enzyme is solved, thus prepares foregoing secretion enzyme liquid, and use it for the processing of potato residues.
3. the manufacture method of alcohol, it is characterised in that to carrying out alcohol by saccharide obtained by the method described in claim 1 or 2
Fermentation.
4. the method described in claim 3, wherein, carry out alcohol fermentation using Blastocystis yeast.
5. the method described in claim 3, it is the ethanol for carrying out alcohol fermentation to foregoing saccharide using Blastocystis yeast
Manufacture method.
6. the method described in claim 3, wherein, fermentative microorganism is carried out with the culture medium of the saccharide containing potato residues
Preceding culture, and carry out alcohol fermentation with the fermentative microorganism cultivated before menstruation.
7. the method described in claim 6, wherein, fermentative microorganism is Blastocystis yeast, and alcohol fermentation is alcohol fermentation.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012037404A JP5843264B2 (en) | 2012-02-23 | 2012-02-23 | Method for saccharification of potato raw material and method for producing liquid fuel |
| JP2012-037404 | 2012-02-23 | ||
| PCT/JP2013/052485 WO2013125336A1 (en) | 2012-02-23 | 2013-02-04 | Method for saccharification of potato starting material and method for producing liquid fuel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104981546A CN104981546A (en) | 2015-10-14 |
| CN104981546B true CN104981546B (en) | 2018-02-13 |
Family
ID=49005525
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380010643.8A Active CN104981546B (en) | 2012-02-23 | 2013-02-04 | The method for saccharifying of raw potatoes and the manufacture method of liquid fuel |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP5843264B2 (en) |
| CN (1) | CN104981546B (en) |
| WO (1) | WO2013125336A1 (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101633938A (en) * | 2009-09-01 | 2010-01-27 | 山西省生物研究所 | Preparation method of fuel alcohol by fermenting waste potato dregs |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4876247B2 (en) * | 2006-04-19 | 2012-02-15 | 国立大学法人山口大学 | A new microorganism belonging to the genus Penicillium |
| CN101041849A (en) * | 2007-04-26 | 2007-09-26 | 湖南南方马铃薯产业化工程技术有限公司 | Method for producing fuel ethanol by using potato as raw material |
| JP5339250B2 (en) * | 2008-07-28 | 2013-11-13 | 独立行政法人産業技術総合研究所 | Method for producing enzyme solution and method for producing sugar |
| JP5629886B2 (en) * | 2010-04-06 | 2014-11-26 | 学校法人東京農業大学 | Fermentation system using ethanol bag and ethanol production method |
-
2012
- 2012-02-23 JP JP2012037404A patent/JP5843264B2/en active Active
-
2013
- 2013-02-04 CN CN201380010643.8A patent/CN104981546B/en active Active
- 2013-02-04 WO PCT/JP2013/052485 patent/WO2013125336A1/en active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101633938A (en) * | 2009-09-01 | 2010-01-27 | 山西省生物研究所 | Preparation method of fuel alcohol by fermenting waste potato dregs |
Non-Patent Citations (2)
| Title |
|---|
| Acremonium cellulolyticus纤维素酶在纤维素乙醇生产中的应用;方诩;《全国生物化工学术、技术交流会论文集》;20100312;摘要及第115页倒数第1段 * |
| Practical Liquefaction of Potato Pulp and Sugar-beet Pulp by Commercial Enzymes;Miyaji等;《东京农大农学集报》;20070920;第52卷(第3期);摘要、第148页左栏倒数第1段、第149页右栏第1段及表2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104981546A (en) | 2015-10-14 |
| JP5843264B2 (en) | 2016-01-13 |
| WO2013125336A1 (en) | 2013-08-29 |
| JP2013172645A (en) | 2013-09-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kolasa et al. | Co-cultivation of Trichoderma reesei RutC30 with three black Aspergillus strains facilitates efficient hydrolysis of pretreated wheat straw and shows promises for on-site enzyme production | |
| da Silva Delabona et al. | Using Amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes | |
| Reddy et al. | Cellulase production by Aspergillus niger on different natural lignocellulosic substrates | |
| Bansal et al. | A novel strain of Aspergillus niger producing a cocktail of hydrolytic depolymerising enzymes for the production of second generation biofuels | |
| CN101555461B (en) | Bacterial strain LT3 producing alkalescence cellulase and breeding method and initial optimization of cellulase production conditions thereof | |
| Naik et al. | Screening of agro-industrial waste and physical factors for the optimum production of pullulanase in solid-state fermentation from endophytic Aspergillus sp. | |
| Han et al. | Solid-state fermentation on poplar sawdust and corncob wastes for lignocellulolytic enzymes by different Pleurotus ostreatus strains | |
| CN102876589B (en) | Strains with high cellulase activity as well as screening method and use method of strains | |
| CN103436569A (en) | Method for preparing sugar and ethanol by using cassava wastes | |
| JP2011050359A (en) | New microorganism, enzyme derived from the microorganism and method for producing saccharified solution by using the same | |
| CN103421851B (en) | A kind of method preparing sugar and ethanol with sweet potato waste | |
| CN101855973A (en) | Fungus strain irpex iacteus for producing laccase, and culturing method and application thereof | |
| Arnthong et al. | Towards a miniaturized culture screening for cellulolytic fungi and their agricultural lignocellulosic degradation | |
| Ogbonna et al. | Isolation of amylase and cellulase producing fungi from decaying tubers and optimization of their enzyme production in solid and submerged cultures | |
| CN113512501B (en) | Penicillium oxalicum XZH-2 and application thereof | |
| Noor El-Deen et al. | Improvement of β-glucosidase production by co-culture of Aspergillus niger and A. oryzae under solid state fermentation through feeding process | |
| Ilić et al. | Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: Screening, hydrolysis, and bioethanol production | |
| Khan et al. | Enhancing a-amylase and cellulase in vivo enzyme expressions on sago pith residue using Bacilllus amyloliquefaciens UMAS 1002 | |
| CN102807958B (en) | Bacterial strain capable of secreting cellulase as well as cellulase extraction method and application thereof | |
| Sharma et al. | Utilization of agro-industrial residues for pectinase production by the novel strain Pseudozyma sp. SPJ under solid state cultivation | |
| Irfan et al. | Influence of nutritional conditions for endoglucanase production by Trichoderma viride in SSF | |
| Zapata et al. | Cellulases production on paper and sawdust using native Trichoderma asperellum | |
| Heng et al. | Effects of different parameters on cellulase production by Trichoderma harzianum TF2 using solid‐state fermentation (SSF) | |
| CN108424896B (en) | Method for producing cellulase by mixed fermentation of corn straw furfural residues | |
| Agrawal et al. | Production of an extracellular cellobiase in solid state fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240305 Address after: Osaka City, Osaka of Japan Patentee after: HITACHI ZOSEN Corp. Country or region after: Japan Address before: Tokyo, Japan Patentee before: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY Country or region before: Japan Patentee before: SOJITZ Corp. Patentee before: HITACHI ZOSEN Corp. |