CN104975055A - Degreasing method for low-ester pectin - Google Patents
Degreasing method for low-ester pectin Download PDFInfo
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- CN104975055A CN104975055A CN201510374281.1A CN201510374281A CN104975055A CN 104975055 A CN104975055 A CN 104975055A CN 201510374281 A CN201510374281 A CN 201510374281A CN 104975055 A CN104975055 A CN 104975055A
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Abstract
The invention discloses a degreasing method for low-ester pectin. According to the degreasing method, pectinase directly acts on a high-ester pectin concentrated solution, so that dosage of pectinase is reduced greatly; by controlling temperature of the concentrated solution, the activity of pectinase is kept, and pectinase plays the role of degreasing; and after degreasing, precipitation is carried out quickly, so that production efficiency is improved greatly, and the production cost is reduced. The degreasing method has the advantages of high extraction efficiency, high production efficiency, high product quality and simple process, and is very suitable for scale production and industrial production. The low-ester pectin production process provided by the invention not only fills the blank of industrially producing low-ester pectin inland, but also breaks the monopoly of foreign manufacturers on low-ester pectin products, industrial development of low-ester pectin in our country is facilitated, and a national pectin brand is built.
Description
Technical field
The present invention relates to a kind of pectin, particularly relate to a kind of de-ester method of low-ester pectin.
Background technology
The de-ester technique of current pectin has: acid system takes off ester, base catalysed de-esterification and enzyme process and takes off ester.
(1) acid system takes off ester: the pectin polygalacturonic acid content obtained is higher, but speed of response is slow, in equality of temperature with under concentration conditions, its de-ester speed is far below base catalysed de-esterification, intensification can fast reaction speed, but the depolymerization speed of pectin chain also can be accelerated greatly, the havoc gel-strength of pectin.
(2) base catalysed de-esterification: its de-ester speed is fast, but pectin is when base catalysed de-esterification, except methoxy content in molecule reduces, also can produce the depolymerization of pectin molecule, i.e. β-elimination reaction simultaneously, cause pectin molecule amount, viscosity and degree of gelation to decline.
(3) enzyme process takes off ester: in the process of pectin extraction, adds polygalacturonase directly de-ester; Shortcoming: the first, because the pectin extraction time is long, causes polygalacturonase considerable damage pectin molecule chain, reduces pectin grade and gamma value, have a strong impact on pectin quality; The second, to producing, the feed quality requirements of pectin is higher, and the low raw material of gel content, in leaching process, causes a large amount of pectin to go to pot because polygalacturonase acts on for a long time, finally cannot extract pectin; 3rd, need higher temperature in leaching process, the havoc activity of polygalacturonase, makes a large amount of polygalacturonases not play corresponding effect, causes polygalacturonase consumption excessive.
Summary of the invention
Instant invention overcomes deficiency of the prior art, provide a kind of de-ester method of low-ester pectin.
The present invention is achieved by the following technical solutions:
A de-ester method for low-ester pectin, comprises the following steps:
(1) one or more in new fresh orange peel, Watermelon rind, Pericarpium Mali pumilae, sunflower plate and bar being crushed to particle diameter is 2-4mm, the raw material obtained mixes its weight 8-10 clear water doubly, be heated to 80-90 DEG C, add the pH to 7-8 of sodium carbonate regulating solution, insulation 1-2 hour;
(2) filtered by above-mentioned solution, the warm water of filter residue 40-50 DEG C that obtains is washed to colorless and odorless repeatedly, adds hydrochloric acid, and regulate pH to 1.5-2, at 60-70 DEG C, extract 30-40 minute, suction filtration obtains filtrate;
(3) filter vacuum is concentrated, obtain the concentrated solution of high ester pectin, in concentrated solution, add ammoniacal liquor regulate pH to 3.5-3.8, the polygalacturonase of the 0.012-0.013% of concentrated liquid measure is added after being cooled to 25-30 DEG C, carry out de-ester reaction, the alcohol adding alcoholic strength 15-20% after 1-2 hour carrys out the activity of passivation polygalacturonase;
(4) add concentrated solution volume 1-1.5 dehydrated alcohol doubly, precipitate centrifugation after 1-2 hour, filter residue spraying dry, obtains low-ester pectin.
Technological principle: the present invention is after producing the concentrated solution of high ester pectin, add high purity pectin enzyme again and carry out de-ester, by controlling the de-ester time, the low-ester pectin product of different gamma value can be generated, the alcohol adding alcoholic strength 15-20% after period terminates carrys out the activity of passivation polygalacturonase, prevent from taking off ester further, effectively control de-ester degree.
Compared with prior art, advantage of the present invention is:
Polygalacturonase is directly acted on high ester pectin concentrated solution by the present invention, greatly reduces the consumption of polygalacturonase; By controlling the temperature of concentrated solution, keeping the activity of polygalacturonase, giving full play to the de-ester effect of polygalacturonase; After completing de-ester, can promptly enter precipitation link, substantially increase production efficiency, reduce production cost.Have that extraction efficiency is high, production efficiency is high, quality product is high, the simple advantage of technique, be very applicable to mass-producing, suitability for industrialized production.Low-ester pectin production technique provided by the invention has not only filled up the blank that domestic industryization produces low-ester pectin, also the technical monopoly of foreign producer to low-ester pectin product has been broken, be conducive to the development promoting China's low-ester pectin industrialization, make national pectin brand.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail:
Embodiment 1: a kind of de-ester method of low-ester pectin, comprises the following steps:
(1) one or more in new fresh orange peel, Watermelon rind, Pericarpium Mali pumilae, sunflower plate and bar being crushed to particle diameter is 2mm, and the raw material obtained mixes the clear water of its weight 8 times, is heated to 90 DEG C, adds the pH to 7 of sodium carbonate regulating solution, is incubated 2 hours;
(2) filtered by above-mentioned solution, the filter residue the obtained warm water of 50 DEG C is washed to colorless and odorless repeatedly, adds hydrochloric acid, regulates pH to 1.5, and extract 30 minutes at 60 DEG C, suction filtration obtains filtrate;
(3) filter vacuum is concentrated, obtain the concentrated solution of high ester pectin, in concentrated solution, add ammoniacal liquor regulate pH to 3.5, after being cooled to 25 DEG C, add the polygalacturonase of 0.0125% of concentrated liquid measure, carry out de-ester reaction, the alcohol adding alcoholic strength 15% after 2 hours carrys out the activity of passivation polygalacturonase;
(4) add the dehydrated alcohol of concentrated solution volume 1.5 times, precipitate centrifugation after 2 hours, filter residue spraying dry, obtains low-ester pectin.
Claims (1)
1. a de-ester method for low-ester pectin, is characterized in that comprising the following steps:
(1) one or more in new fresh orange peel, Watermelon rind, Pericarpium Mali pumilae, sunflower plate and bar being crushed to particle diameter is 2-4mm, the raw material obtained mixes its weight 8-10 clear water doubly, be heated to 80-90 DEG C, add the pH to 7-8 of sodium carbonate regulating solution, insulation 1-2 hour;
(2) filtered by above-mentioned solution, the warm water of filter residue 40-50 DEG C that obtains is washed to colorless and odorless repeatedly, adds hydrochloric acid, and regulate pH to 1.5-2, at 60-70 DEG C, extract 30-40 minute, suction filtration obtains filtrate;
(3) filter vacuum is concentrated, obtain the concentrated solution of high ester pectin, in concentrated solution, add ammoniacal liquor regulate pH to 3.5-3.8, the polygalacturonase of the 0.012-0.013% of concentrated liquid measure is added after being cooled to 25-30 DEG C, carry out de-ester reaction, the alcohol adding alcoholic strength 15-20% after 1-2 hour carrys out the activity of passivation polygalacturonase;
(4) add concentrated solution volume 1-1.5 dehydrated alcohol doubly, precipitate centrifugation after 1-2 hour, filter residue spraying dry, obtains low-ester pectin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510374281.1A CN104975055A (en) | 2015-07-01 | 2015-07-01 | Degreasing method for low-ester pectin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510374281.1A CN104975055A (en) | 2015-07-01 | 2015-07-01 | Degreasing method for low-ester pectin |
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| CN104975055A true CN104975055A (en) | 2015-10-14 |
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| CN201510374281.1A Pending CN104975055A (en) | 2015-07-01 | 2015-07-01 | Degreasing method for low-ester pectin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108715618A (en) * | 2018-04-13 | 2018-10-30 | 安徽宇宁果胶股份有限公司 | A kind of production method of the special pectin of colloidal bismuth pectin |
| CN111004788A (en) * | 2019-12-17 | 2020-04-14 | 中国农业科学院麻类研究所 | Pectin esterase and preparation method and application thereof |
| CN112266941A (en) * | 2020-09-30 | 2021-01-26 | 浙江果源康品生物科技有限公司 | Method for producing micromolecular pectin by combining enzyme and alkali |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174611A (en) * | 2010-12-27 | 2011-09-07 | 安徽金枫果胶有限公司 | Process for degreasing low methoxyl pectin |
| CN102260723A (en) * | 2010-05-24 | 2011-11-30 | 淄博中轩生化有限公司 | Technology for producing low fat pectin with enzymatic method |
-
2015
- 2015-07-01 CN CN201510374281.1A patent/CN104975055A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102260723A (en) * | 2010-05-24 | 2011-11-30 | 淄博中轩生化有限公司 | Technology for producing low fat pectin with enzymatic method |
| CN102174611A (en) * | 2010-12-27 | 2011-09-07 | 安徽金枫果胶有限公司 | Process for degreasing low methoxyl pectin |
Non-Patent Citations (1)
| Title |
|---|
| JUN CHEN 等: "Extraction of pectin from Premna microphylla turcz leaves and its physicochemical properties", 《CARBOHYDRATE POLYMERS》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108715618A (en) * | 2018-04-13 | 2018-10-30 | 安徽宇宁果胶股份有限公司 | A kind of production method of the special pectin of colloidal bismuth pectin |
| CN111004788A (en) * | 2019-12-17 | 2020-04-14 | 中国农业科学院麻类研究所 | Pectin esterase and preparation method and application thereof |
| CN112266941A (en) * | 2020-09-30 | 2021-01-26 | 浙江果源康品生物科技有限公司 | Method for producing micromolecular pectin by combining enzyme and alkali |
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Application publication date: 20151014 |
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