CN104971935A - Technological method for innocent treatment of animals dying from diseases through subcritical water - Google Patents
Technological method for innocent treatment of animals dying from diseases through subcritical water Download PDFInfo
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Abstract
The invention discloses a technological method for innocent treatment of animals dying from diseases through subcritical water. The technological method includes the steps that crushed animal carcasses are sealed after the addition of purified water; the animal carcasses are put at the temperature of 170-240 DEG C and the pressure of 0.5-3.6 Mpa, and the temperature and the pressure are kept for certain time; preferably, the temperature and the pressure are kept for 1-2 hours. According to the technological method for innocent treatment of the animals dying from diseases through subcritical water, the animals dying from diseases and waste of the animals are used, and therefore various liquid amino acid with high yield can be prepared through the method. The technological method is low in cost, convenient to implement and simple, the environment and the waste are utilized, and the technological method is worthy of being popularized greatly.
Description
Technical field
The present invention relates to animals died of illness harmless treatment process technical field, particularly relate to a kind of process utilizing subcritical water harmless treatment animals died of illness.
Background technology
Animals died of illness, as a kind of alternative materials of fossil feedstock, because it has resource extensively and the feature such as recyclability, more and more causes the attention of people " current expired animals died of illness is processed by as discarded object ".Present stage, in dead livestock and poultry harmless treatment, apply more technology and mainly comprise buried method, burning method, composting process, change corpse cellar for storing things facture, change the processing method such as method for making, biological degradation method.(1) buried method: refer to by utilizing the self purification of soil to make it innoxious by the method for burying.But because its innoxious process is slow, likely contaminated soil or underground water.In addition, this law is not suitable for the process of epidemic-infected animal and product, tissue.(2) burning method: refer to and the livestock and poultry died of illness are deposited on enough fuel things or are placed in incinerator, within the shortest time, realize livestock and poultry corpse to burn completely carbonization, but a large amount of pollutant (flue gas) can be produced in process, simultaneously in combustion process if any imperfect combustion organic matter, can to environment.(3) spoil compost: refer to and spoil is placed in compost inside, by the metabolic process degraded spoil of microorganism, but may cause the diffusion of pathogenic microorganism, also can pollute turning device simultaneously, even infect turning personnel.Frequent turning can upset flora around spoil in addition, the degraded of interference animal tissue.(4) change corpse cellar for storing things: namely with the change corpse of appropriate volume cellar for storing things deposition spoil, allow its naturally rot degrade method.Can not be cycled to repeat utilization, natural degradation process affects very large by season, regional temperature.(5) biodegradation refers to and puts in degradation reaction device by animals died of illness corpse, utilize the fermentative degradation principle of microorganism, by the process of the fragmentation of animals died of illness corpse, degraded, sterilizing, but need to add auxiliary material and biological bacteria, processing cost is high, and treating capacity is little.(6) process of change method for making refers to and puts in hydrolysis tank by animals died of illness corpse, animals died of illness corpse is cleared up and is converted into aseptic aqueous solution (amino acid is main) and dry bone slag, simultaneously by process that all pathogenic microorganisms are thoroughly killed.But existing chemical hydrolysis and enzymatic isolation method processing procedure easily produce foul gas (peculiar smell is obvious) and waste water.
How reasonably recycling animals died of illness and a discarded object thereof! Increase technology content! Improve added value! Reduce production cost! Reduce environmental pollution, make it resource, real realization is turned waste into wealth, and has very important actual production meaning.And animals died of illness and discarded object thereof to be hydrolyzed into high value-added product be a kind of real effective method, just seem particularly important so find and develop a kind of eco-friendly method for hydrolysis completely newly to the shortcoming overcoming chemical hydrolysis and enzymolysis.
Subcritical technology is a kind of new and high technology that development in recent years is got up, have broad application prospects at field of environment protection, utilize it the organic pollution of difficult degradation in waste water can be rapidly decomposed into small-molecule substance, as CO2, H2O etc., and decompose completely, any secondary pollution can not be caused " compared with traditional solvent to environment, subcritical not only in ecology, economical, the aspects such as safety have certain advantage, and due to its density, ionic product and dielectric constant etc. can be regulated by pressure and temperature, it is a kind of suitable reaction medium, therefore the chemical reaction in subcritical water causes extensive attention.
Summary of the invention
The object of the present invention is to provide a kind of low cost, convenient and simple, environmental protection, the method for the utilization dead spoil production Liquid amino acid of twice laid.
For achieving the above object, the invention provides a kind of process utilizing subcritical water harmless treatment animals died of illness, it is characterized in that, step is, seals after spoil after fragmentation is added pure water; In 170-240 DEG C, 0.5-3.6Mpa, and keep temperature, pressure certain hour; Preferably, temperature, pressure 1-2 hour is kept.
Further, step is, by spoil chopping to being less than or equal to 15cm, drops into high-temperature high pressure water solution reaction kettle; Seal after adding pure water; Design temperature is 170-240 DEG C, and pressure is start reactor after 0.5-3.6Mpa to start heating; Reach after 100 DEG C, open vent valve, after the air in emptying reactor, close emptying valve and keep airtight, during to design temperature, make pure water be in subcritical state, and keep certain hour; Preferably, 1-2 hour is kept; When off-response still makes it naturally cool to normal pressure, discharging.
Further, step is, by spoil chopping to being less than or equal to 15cm, drops into high-temperature high pressure water solution reaction kettle; Seal after adding pure water; Design temperature is 170-240 DEG C, and pressure is start reactor after 0.5-3.6Mpa to start heating; Reach after 100 DEG C, open vent valve, after air in emptying reactor, close emptying valve and keep airtight, during to design temperature, pure water is made to be in subcritical state, and keep 15 minutes, after carrying out first time pressure release to normal pressure, close pressure relief opening and keep reactor airtight, continue to be heated to design temperature and keep 15 minutes, carrying out second time pressure release; When falling back to normal pressure Deng reactor, discharging.
Further, step is, is shredded by spoil, drops into reactor; Seal after adding pure water; Carbon dioxide is squeezed into from air inlet; Design temperature is 170-220 DEG C, and pressure is start reactor after 0.5-2.6Mpa to start heating; During to design temperature, keep 1-2 hour, when off-response still makes it naturally cool to normal pressure, discharging.
Further, described animal is the animal died of illness.Further, described animal is pig, ox, horse, sheep, chicken, dog or cat.
Due to animals died of illness resource extensively and renewable, available animals died of illness is raw material, is middlely hydrolyzed to high value-added product or the raw material of industry subcritical; Some be there is no to the discarded object of recycle value, can make it to decompose completely in subcritical, reduce their pollutions to environment; Can the discarded object of recycling for those, can make it in subcritical middle hydrolysis or be degraded to industrial chemicals or intermediate etc. thus have found the green approach processing solid biologic discarded object, for the recycling of animals died of illness and discarded object thereof opens new approach.The features such as animals died of illness and discarded object thereof are hydrolyzed into amino acid whose method and have pollution-free in near-critical water, catalyst-free, efficient, have good application prospect "
Invention has been the research that animals died of illness and discarded object thereof are hydrolyzed in near-critical water, make large biological molecule, as protein, fat etc., in near-critical water, hydrolysis generates biological micromolecule, as the industrial chemicals such as amino acid, organic acid or intermediate, not only solve animals died of illness surplus and problem of environmental pollution, and resource is fully used, produce larger economic benefit.
Applicant's technological experiment result of the present invention shows: affect animals died of illness and be hydrolyzed into amino acid whose factor and mainly contain four aspects, i.e. reaction temperature, reaction pressure, reaction time and reaction atmosphere, if temperature is too low, then the subcritical character of water just can not be accomplished, and temperature is too high, then can make again the too high generation hydrolysis of amino acid Yin Wendu or other side reaction, so, find suitable temperature, be improve of hydrolysate productive rate main because of, the size of pressure also can affect amino acid whose content in hydrolyzate to a certain extent, in general, pressure is larger, content will be higher, but consider the condition such as equipment and power, so pressure cannot control get Tai Gao " reaction time can not be too short, also unsuitable long, the incomplete product yield of too short hydrolysis is low, longly can reduce production efficiency.
Embodiments of the invention fully demonstrate, and the present invention can take to be low to moderate 170 DEG C, and pressure also can be low to moderate the situation of 0.5Mpa, and the amino acid whose yield obtained is still very high, greatly reduces production cost.
Detailed description of the invention
Be described below in detail embodiments of the invention, the example of described embodiment is intended to for explaining the present invention, and can not be interpreted as limitation of the present invention.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition or carry out according to product description.Agents useful for same or the unreceipted production firm person of instrument, being can by the conventional products of commercial acquisition.
Embodiment 1: prepare Liquid amino acid with dead pig
1, clean GSH-10L reactor, connect power supply etc., prepare before carrying out experiment;
2, sick dead pig is shredded, make its raw meal particle size be less than or equal to 15cm;
3, by the pig 1.46KG of chopping, reactor is dropped into;
4, pure water 0.52KG is added;
5, sealed reactor is bolted on;
6, temperature sets 170 DEG C, and pressure setting 0.5Mpa starts reactor and starts heating;
7, observe temperature, reach after 100 DEG C, open vent valve, the air in emptying reactor;
8, observed and recorded parameter, after equitemperature reaches 170 DEG C, keep 1.5 hours, off-response still makes it naturally cool;
9, etc. when reactor pressure falls back to normal pressure, discharging.
The hydrolyzate amino-acid analyzer analysis of final acquisition amino acid classes wherein and productive rate, the results are shown in Table 1.
Table 1 the present embodiment amino acid classes and productive rate table
| Amino acid | Productive rate (g/L) |
| Aspartic acid | 0.25 |
| Threonine | 0.07 |
| Serine | 0.35 |
| Glutamic acid | 0.09 |
| Glycine | 1.17 |
| Alanine | 0.79 |
| Cystine | 0.74 |
| Valine | 0.06 |
| Methionine | 0.05 |
| Isoleucine | 0.15 |
| Leucine | 0.12 |
| Tyrosine | 0.1 |
| Phenylalanine | 0.36 |
| Histidine | 0.05 |
| Lysine | 0.12 |
| Arginine | 0.22 |
| Proline | 0.24 |
| Total amount | 4.93 |
From the results shown in Table 1, sick dead pig, at 170 DEG C, can start under the condition of 0.5MPa to be hydrolyzed into Liquid amino acid, wherein higher with glycine ratio.
Embodiment two: prepare Liquid amino acid with dead pig
1, clean GSH-10L reactor, connect power supply etc., prepare before carrying out experiment;
2, sick dead pig is shredded, make its raw meal particle size be less than or equal to 15cm;
3, get the dead pig 3.905KG after chopping, drop into reactor;
4, pure water 1.755KG is added;
5, sealed reactor is bolted on;
6, temperature setting: 200 DEG C, pressure setting 1.6Mpa, starts reactor and starts heating;
7, observe temperature, reach after 100 DEG C, open vent valve, the air in emptying reactor;
8, observed and recorded parameter, after equitemperature reaches 200 DEG C, keep 1.5 hours, off-response still makes it naturally cool;
9, etc. when reactor pressure falls back to normal pressure, discharging.
The hydrolyzate amino-acid analyzer analysis of final acquisition amino acid classes wherein and productive rate, the results are shown in Table 2.
Table 2 the present embodiment amino acid classes and productive rate table
| Amino acid | Productive rate (g/L) |
| Aspartic acid | 0.1 |
| Threonine | 0.04 |
| Serine | 0.02 |
| Glutamic acid | 1.95 |
| Glycine | 0.91 |
| Alanine | 0.05 |
| Cystine | 0.67 |
| Valine | 0.04 |
| Methionine | 0.02 |
| Isoleucine | 0.26 |
| Leucine | 0.1 |
| Tyrosine | 0.13 |
| Phenylalanine | 0.27 |
| Histidine | 0.21 |
| Lysine | 0.11 |
| Arginine | 0.21 |
| Proline | 0.24 |
| Total amount | 5.33 |
From the results shown in Table 2, rise to 200 DEG C along with temperature and pressure, can find out during 1.6MPa, the productive rate that sick dead pig is hydrolyzed into Liquid amino acid increases, wherein higher with glutamic acid ratio.
Embodiment 3: prepare Liquid amino acid with dead pig
1, clean GSH-10L reactor, connect power supply etc., prepare before carrying out experiment;
2, sick dead pig is shredded, make its raw meal particle size be less than or equal to 15cm;
3, by the pig 2.7KG of chopping, reactor is dropped into;
4, pure water 1.415KG is added;
5, sealed reactor is bolted on;
6, temperature setting: 220 DEG C, pressure setting 2.6Mpa starts reactor and starts heating;
7, observe temperature, reach after 100 DEG C, open vent valve, the air in emptying reactor;
8, observed and recorded parameter, after equitemperature reaches 220 DEG C, keep 1.5 hours, off-response still makes it naturally cool;
9, etc. when reactor pressure falls back to normal pressure, discharging.
The hydrolyzate amino-acid analyzer analysis of final acquisition amino acid classes wherein and productive rate, the results are shown in Table 3.
Table 3 the present embodiment amino acid classes and productive rate table
| Amino acid | Productive rate (g/L) |
| Aspartic acid | 0.02 |
| Threonine | 0.02 |
| Serine | 0.03 |
| Glutamic acid | 2.2 |
| Glycine | 0.91 |
| Alanine | 0.05 |
| Cystine | 0.88 |
| Valine | 0.06 |
| Methionine | 0.03 |
| Isoleucine | 0.41 |
| Leucine | 0.09 |
| Tyrosine | 0.11 |
| Phenylalanine | 0.18 |
| Histidine | 0.24 |
| Lysine | 0.14 |
| Arginine | 0.15 |
| Proline | 0.31 |
| Total amount | 5.83 |
From the results shown in Table 3, be increased to 220 DEG C along with the setting value by reaction temperature and pressure, can find out during 2.6MPa, the productive rate that sick dead pig is hydrolyzed into Liquid amino acid increases, wherein higher with glutamic acid ratio.
Embodiment 4: prepare Liquid amino acid with dead pig
1, clean GSH-10L reactor, connect power supply etc., prepare before carrying out experiment;
2, sick dead pig is shredded, make its raw meal particle size be less than or equal to 15cm;
3, by the pig 2.69KG of chopping, reactor is dropped into;
4, pure water 1.42KG is added;
5, sealed reactor is bolted on;
6, carbon dioxide is squeezed into from air inlet;
7, temperature setting: 200 DEG C, pressure setting 1.6Mpa starts reactor and starts heating;
8, observed and recorded parameter, after equitemperature reaches 200 DEG C, keep 1.5 hours, off-response still makes it naturally cool;
9, etc. when reactor pressure falls back to normal pressure, discharging.
The hydrolyzate amino-acid analyzer analysis of final acquisition amino acid classes wherein and productive rate, the results are shown in Table 4.
Table 4 the present embodiment amino acid classes and productive rate table
| Amino acid | Productive rate (g/L) |
| Aspartic acid | 0.05 |
| Threonine | 0.01 |
| Serine | 0.01 |
| Glutamic acid | 2.13 |
| Glycine | 0.9 |
| Alanine | 0.05 |
| Cystine | 0.77 |
| Valine | 0.06 |
| Methionine | 0.02 |
| Isoleucine | 0.37 |
| Leucine | 0.1 |
| Tyrosine | 0.11 |
| Phenylalanine | 0.21 |
| Histidine | 0.23 |
| Lysine | 0.13 |
| Arginine | 0.1 |
| Proline | 0.26 |
| Total amount | 5.5 |
From the results shown in Table 4, when adding the assist gas such as carbon dioxide, the productive rate that sick dead pig is hydrolyzed into Liquid amino acid can increase, wherein higher with glutamic acid ratio.
Embodiment 5: prepare Liquid amino acid with dead pig
1, clean GSH-10L reactor, connect power supply etc., prepare before carrying out experiment;
2, sick dead pig is shredded, make its raw meal particle size be less than or equal to 15cm;
3, cleaning reaction still, connects power supply etc., prepares before carrying out experiment;
4, by the pig 1.635KG of chopping, reactor is dropped into;
5, pure water 0.81KG is added;
6, sealed reactor is bolted on;
7, carbon dioxide is squeezed into from air inlet;
8, temperature sets 220 DEG C, and pressure setting 2.6Mpa starts reactor and starts heating;
9, observed and recorded parameter, after equitemperature reaches 220 DEG C, keep 1.5 hours, off-response still makes it naturally cool;
8, etc. when reactor pressure falls back to normal pressure, discharging.
The hydrolyzate amino-acid analyzer analysis of final acquisition amino acid classes wherein and productive rate, the results are shown in Table 5.
Table 5 the present embodiment amino acid classes and productive rate table
| Amino acid | Productive rate (g/L) |
| Aspartic acid | 0.02 |
| Threonine | 0.01 |
| Serine | 0.02 |
| Glutamic acid | 2.14 |
| Glycine | 0.87 |
| Alanine | 0.04 |
| Cystine | 0.99 |
| Valine | 0.06 |
| Methionine | 0.03 |
| Isoleucine | 0.4 |
| Leucine | 0.1 |
| Tyrosine | 0.13 |
| Phenylalanine | 0.15 |
| Histidine | 0.2 |
| Lysine | 0.14 |
| Arginine | 0.14 |
| Proline | 0.29 |
| Total amount | 5.73 |
From the results shown in Table 5, at 220 DEG C, when adding the assist gas such as carbon dioxide under 2.6MPa equal conditions, the productive rate that sick dead pig is hydrolyzed into Liquid amino acid remains basically stable or has and reduces by a small margin, wherein higher with glutamic acid ratio.
Embodiment 6: prepare Liquid amino acid with dead pig
1. clean GSH-10L reactor, connect power supply etc., prepare before carrying out experiment;
2. sick dead pig is shredded, make its raw meal particle size be less than or equal to 15cm;
3. adding pure water makes it not have pork
4. sealed reactor is bolted on
5. temperature sets 240 DEG C, and pressure setting 3.6Mpa starts reactor and starts heating;
6. start reactor and start heating
7. observe temperature, reach after 100 DEG C, open vent valve, the air in emptying reactor
8. observed and recorded parameter, after equitemperature reaches 240 DEG C, keep 1.5 hours, off-response still makes it naturally cool
9. when pressure such as reactor such as grade falls back to normal pressure, discharging
The hydrolyzate amino-acid analyzer analysis of final acquisition amino acid classes wherein and productive rate, the results are shown in Table 6.
Table 6 the present embodiment amino acid classes and productive rate table
From the results shown in Table 6, at 240 DEG C, 3.6MPa, the productive rate that sick dead pig is hydrolyzed into Liquid amino acid increases substantially, wherein higher with glycine ratio.
Embodiment 7: prepare Liquid amino acid with dead pig
1. clean GSH-10L reactor, connect power supply etc., prepare before carrying out experiment;
2. sick dead pig is shredded, make its raw meal particle size be less than or equal to 15cm;
3. adding pure water makes it not have pork
4. sealed reactor is bolted on
5. temperature sets 240 DEG C, and pressure setting 3.6Mpa starts reactor and starts heating;
6. start reactor and start heating;
7. observe temperature, reach after 100 DEG C, open vent valve, the air in emptying reactor;
8. observed and recorded parameter, after equitemperature reaches 240 DEG C, keeps 15min, starts first time pressure release, make it to normal pressure; Closing pressure relief opening keeps reactor airtight, continues to be heated to design temperature, and keeps 15min, carry out second time pressure release; When falling back to normal pressure Deng reactor, discharging.
The hydrolyzate amino-acid analyzer analysis of final acquisition amino acid classes wherein and productive rate, the results are shown in Table 7.
Table 7 the present embodiment amino acid classes and productive rate table
| Amino acid | Productive rate (g/L) |
| Aspartic acid | 0.06 |
| Threonine | 0.02 |
| Serine | 0.07 |
| Glutamic acid | 0.10 |
| Glycine | 3.12 |
| Alanine | 2.97 |
| Cystine | 0.72 |
| Valine | 0.72 |
| Methionine | 0.22 |
| Isoleucine | 0.18 |
| Leucine | 0.77 |
| Tyrosine | 0.32 |
| Phenylalanine | 0.33 |
| Histidine | 0.41 |
| Lysine | 0.39 |
| Arginine | 0.05 |
| Proline | 1.49 |
| Total amount | 11.94 |
From the results shown in Table 7, at 240 DEG C, 3.6MPa, the productive rate that during pressure release, sick dead pig is hydrolyzed into Liquid amino acid at twice improves greatly, wherein higher with glycine, alanine ratio.
Although illustrate and describe embodiments of the invention above, be understandable that, above-described embodiment is exemplary, can not be interpreted as limitation of the present invention, those of ordinary skill in the art can change above-described embodiment within the scope of the invention when not departing from principle of the present invention and aim, revising, replacing and modification.
Claims (6)
1. utilize a process for subcritical water harmless treatment animals died of illness, it is characterized in that, step is, seals after spoil after fragmentation is added pure water; In 170-240 DEG C, 0.5-3.6Mpa, and keep temperature, pressure certain hour; Preferably, temperature, pressure 1-2 hour is kept.
2. utilize the process of subcritical water harmless treatment animals died of illness described in claim 1, it is characterized in that, step is, by spoil chopping to being less than or equal to 15cm, drops into high-temperature high pressure water solution reaction kettle; Seal after adding pure water; Design temperature is 170-240 DEG C, and pressure is start reactor after 0.5-3.6Mpa to start heating; Reach after 100 DEG C, open vent valve, after the air in emptying reactor, close emptying valve and keep airtight, during to design temperature, make pure water be in subcritical state, and keep certain hour; Preferably, 1-2 hour is kept; When off-response still makes it naturally cool to normal pressure, discharging.
3. utilize the process of subcritical water harmless treatment animals died of illness described in claim 1, it is characterized in that, step is, by spoil chopping to being less than or equal to 15cm, drops into high-temperature high pressure water solution reaction kettle; Seal after adding pure water; Design temperature is 170-240 DEG C, and pressure is start reactor after 0.5-3.6Mpa to start heating; Reach after 100 DEG C, open vent valve, after air in emptying reactor, close emptying valve and keep airtight, during to design temperature, pure water is made to be in subcritical state, and keep 15 minutes, after carrying out first time pressure release to normal pressure, close pressure relief opening and keep reactor airtight, continue to be heated to design temperature and keep 15 minutes, carrying out second time pressure release; When falling back to normal pressure Deng reactor, discharging.
4. utilize the process of subcritical water harmless treatment animals died of illness described in claim 1, it is characterized in that, step is, is shredded by spoil, drops into reactor; Seal after adding pure water; Carbon dioxide is squeezed into from air inlet; Design temperature is 170-220 DEG C, and pressure is start reactor after 0.5-2.6Mpa to start heating; During to design temperature, keep 1-2 hour, when off-response still makes it naturally cool to normal pressure, discharging.
5. utilize the process of subcritical water harmless treatment animals died of illness described in claim 1, it is characterized in that, described animal is the animal died of illness.
6. utilize the process of subcritical water harmless treatment animals died of illness described in claim 1, it is characterized in that, described animal is pig, ox, horse, sheep, chicken, dog or cat.
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| CN108610265A (en) * | 2016-12-12 | 2018-10-02 | 佳纶生技股份有限公司 | Manufacturing method containing amino acid product |
| CN109570200A (en) * | 2018-12-10 | 2019-04-05 | 厦门康浩科技有限公司 | A kind of device and method using subcritical water harmless treatment animals died of illness |
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Application publication date: 20151014 |