CN104969771A - High-cordycepin-content cordyceps militaris strain breeding technology - Google Patents

Abstract

The invention provides a high-cordycepin-content cordyceps militaris strain breeding technology. The technology is characterized in that cordyceps mycelium of wild cordyceps militaris successfully developed by the designer used for preparing a high-cordycepin-content cordyceps militaris mycelium liquid 1; a high-cordycepin-content cordyceps militaris liquid mother strain culture solution 2 is prepared; the wild cordyceps militaris cordyceps mycelium liquid is inoculated in the high-cordycepin-content cordyceps militaris liquid mother strain culture solution, and reciprocating shaking culture is carried out, such that a high-cordycepin-content cordyceps militaris strain 3 is obtained. The technology is applied in the production process with a high-cordycepin-content cordyceps militaris strain breeding technology. The technology can bringing in significant economic and social benefits if widely applied.

Description

High plain Cordyceps militaris spawn breeding technique

Technical field

The present invention is a kind of breeding technique of edible bacterium.

Background technology

Cordyceps militaris manually carries training for many years in China, it is all utilize fruiting bodies of cordyceps militaris to be separated female kind and do not have the requirement of cordycepin content to being separated fruit body used that present China Cordyceps militaris carries training, it is extremely low that this makes to produce year training fruiting bodies of cordyceps militaris cordycepin content out, this makes the health care function of Cordyceps militaris receive significant limitation, people expect to have a novel high cordycepin Cordyceps militaris spawn always and breeding technique comes out, and this is insurmountable problem always for a long time.

Height provided by the invention plain Cordyceps militaris spawn breeding technique overcomes the deficiency of Cordyceps militaris breeding technique traditional for many years in cordycepin content requires etc., for people provide a kind of height element Cordyceps militaris spawn breeding technique of novelty of science, thus change the backward situation of Cordyceps militaris breeding, for people provide a whole set of high plain Cordyceps militaris spawn breeding technique and product.

Summary of the invention

The present invention be designer oneself is succeeded in developing add sterile water 100 milliliter at 0.5%-0.99% with the polypide mycelia 5 grams of wild cordyceps militaris with cordycepin content, high plain Cordyceps militaris wild polypide mycelia liquid 1 is stirred under aseptic condition, directly cultivate high plain Cordyceps militaris mother with height plain wild cordyceps militaris polypide mycelia liquid to plant, liquid Mother culture formula of liquid is peptone 5 grams, soluble starch 10 grams, royal jelly 10 grams, 1000 milliliters, water, high plain Cordyceps militaris parent culture fluid 2 is after conventional method sterilizing cooling, aseptically access high plain wild cordyceps militaris polypide mycelia liquid 5 milliliters of reciprocating shaken cultivation with 250 milliliters of bottled 125 milliliters high plain Cordyceps militaris Mother culture liquid of triangle within 96 hours, to be the technology such as high plain Cordyceps militaris spawn 3 and to be applied among the production technology of high plain Cordyceps militaris spawn breeding technique, the present invention will produce obvious economic benefit and social benefit if be used widely.

Accompanying drawing explanation

Accompanying drawing is high plain Cordyceps militaris spawn breeding technique production operation schematic flow sheet, in figure, 1 for adding sterile water 100 milliliter at 0.5%-0.99% with the polypide mycelia 5 grams of wild cordyceps militaris with cordycepin content, high plain Cordyceps militaris wild polypide mycelia liquid is stirred under aseptic condition, 2 plant for directly cultivating high plain Cordyceps militaris mother with high plain wild cordyceps militaris polypide mycelia liquid, liquid Mother culture formula of liquid is peptone 5 grams, soluble starch 10 grams, royal jelly 10 grams, 1000 milliliters, water, high plain Cordyceps militaris parent culture fluid is after conventional method sterilizing cooling, 3 are high plain Cordyceps militaris spawn for 96 hours for aseptically accessing high plain wild cordyceps militaris polypide mycelia liquid 5 milliliters of reciprocating shaken cultivation with 250 milliliters of bottled 125 milliliters high plain Cordyceps militaris Mother culture liquid of triangle.

Embodiment:

The plain Cordyceps militaris spawn breeding technique of height provided by the invention, implement as shown in drawings, sterile water 100 milliliters is added at 0.5%-0.99% with the polypide mycelia 5 grams of wild cordyceps militaris with cordycepin content, high plain Cordyceps militaris wild polypide mycelia liquid 1 is stirred under aseptic condition, directly cultivate high plain Cordyceps militaris mother with height plain wild cordyceps militaris polypide mycelia liquid to plant, liquid Mother culture formula of liquid is peptone 5 grams, soluble starch 10 grams, royal jelly 10 grams, 1000 milliliters, water, high plain Cordyceps militaris parent culture fluid 2 is after conventional method sterilizing cooling, aseptically access high plain wild cordyceps militaris polypide mycelia liquid 5 milliliters of reciprocating shaken cultivation with 250 milliliters of bottled 125 milliliters high plain Cordyceps militaris Mother culture liquid of triangle and within 96 hours, be high plain Cordyceps militaris spawn 3.

Claims (3)

1. add sterile water 100 milliliter at 0.5%-0.99% with the polypide mycelia 5 grams of wild cordyceps militaris with cordycepin content, under aseptic condition, stir into high plain Cordyceps militaris wild polypide mycelia liquid.

2. directly cultivate high plain Cordyceps militaris mother with high plain wild cordyceps militaris polypide mycelia liquid to plant, liquid Mother culture formula of liquid is peptone 5 grams, soluble starch 10 grams, royal jelly 10 grams, 1000 milliliters, water, is high plain Cordyceps militaris parent culture fluid after conventional method sterilizing cooling.

3. aseptically access high plain wild cordyceps militaris polypide mycelia liquid 5 milliliters of reciprocating shaken cultivation with 250 milliliters of bottled 125 milliliters high plain Cordyceps militaris Mother culture liquid of triangle and within 96 hours, be high plain Cordyceps militaris spawn.