CN104962468A - Epiphytic diatom culture and collection device - Google Patents
Epiphytic diatom culture and collection device Download PDFInfo
- Publication number
- CN104962468A CN104962468A CN201510384155.4A CN201510384155A CN104962468A CN 104962468 A CN104962468 A CN 104962468A CN 201510384155 A CN201510384155 A CN 201510384155A CN 104962468 A CN104962468 A CN 104962468A
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- diatom
- cage
- water
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- buoyancy aid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Abstract
The invention relates to an epiphytic diatom culture and collection device. The culture and collection device comprises an on-water floating body, a suspending cage, culture medium, a stainless steel wire rope, an anchor device, a porcelain dish, a brush, a narrow-mouthed bottle, formaldehyde reagent and the like, wherein the suspending cage is installed with a water temperature and flow rate integrated intelligent remote sensor; a signboard is placed at the top of the on-water floating body, the floating body is installed with a signal acquisition and transmitting device and a power supply, and water temperature and flow rate are monitored through wireless remote monitoring and are transmitted to a data center through wireless transmission. The diatom culture and collection device disclosed by the invention can flexibly and conveniently realize the culture and collection of epiphytic diatom in rivers and lakes which lack for qualified sampling medium, and can acquire the water temperature and flow rate of the region on line according to the needs.
Description
Technical field
The present invention relates to the cultivation of the water quality biological monitoring equipment, particularly Attached diatom in a kind of river, lake and acquisition method and equipment.
Background technology
The water quality biological Monitoring techniques in river and lake is a kind of technological method pressing close to human health responding to water pollutant situation based on biology, and the monitoring of diatom water quality biological belongs to this category.The diatom that grows nonparasitically upon another plant refers to the attached microorganism grown on rock or emergent, and the sample relating to diatom in diatom water quality monitoring (WQM) is chosen and gathered.Very high to the requirement of sampling position, sampling matrix in diatom water quality monitoring (WQM) code, and require that sample area has certain flow velocity; Illumination is sufficient; The depth of water is 0.3 ~ 0.8 meter of the best; Riverbed is the few non sludge of sandstone; The nature culture that matrix preferably uses natural sillar or ovum rock etc. stable.Experiment proves to take diatom biological sample well can reflect local water quality condition by aforesaid method.
Above-mentioned matrix is difficult to find in some river, and artificial matrices is easily subject to the factor such as resident, weather conditions impact along the river; Even if do not affect by above-mentioned, the change of sampled result depends primarily on the length of matrix soaking time, and the impact of change on sampled result of water level be can not ignore.In lake, this phenomenon is more outstanding, if the sample gathered contains earth, very large on experimental result impact, now failed sample.
Summary of the invention
The present invention proposes a kind of simple and easy cultivation and gathering device, has water temperature and flow velocity integrated intelligent remotely monitored sensor and supporting signal wireless projector concurrently.The invention solves the drawback that experimenter cannot collect qualified water biological sample in rivers and lakes, overcome disadvantageous outside atmosphere simultaneously, effectively can carry out biological monitoring sample cultivation and the collection of rivers and lakes.For achieving the above object, the present invention by the following technical solutions:
One grow nonparasitically upon another plant diatom cultivate and gathering device, described cultivation and gathering device comprise water floating body 7, and there is bolt interface buoyancy aid 7 upper end, connection department monitoring direction board 10; Growing nonparasitically upon another plant diatom incubator 6 in lower end, only welds when a position 4, four side weld connects and weld four positions 4 when being welded on middle part.Incubator 6 adopts rectangular parallelepiped cage, and developing medium 1 to insert in cage 6 on request; Padlock 13 on diatom culture apparatus is connected to bank with stainless steel wire rope 11, and is fixed by ground tackle, to facilitate sampling.During diatom sampling, naturally the hard stone 1 cultivating more than 1 month in cage 6 is taken out, with brush 16, the diatom brush of growing nonparasitically upon another plant of sunny slope in medium 1 is entered in porcelain dish 15, the surperficial diatom brush that faced south by medium 1 as far as possible when sampling enters in 15, and load in narrow-mouthed bottle 14 by brush 16, and wash away totally with pure water, guarantee that brushing the diatom sample got all loads in bottle 14, the alcohol or the formaldehyde formulations that finally add final concentration 10% are preserved.According to the needs of water quality monitoring (WQM), can select to install water temperature and flow velocity integrated intelligent remotely monitored sensor 2, auxiliary facility such as installation signals collecting and projector etc. 9 buoyancy aid 7 in, water temperature and flow velocity are by radio telecontrol monitoring and be wirelessly transmitted to Data centre.
The feature of the technical program is: for great rivers, the river of the few stone of high concentration of sediment, lake, exist and be difficult to collect qualified diatom sample, or adopt the defect that diatom sample can not represent the actual water quality condition in this region, the diatom water quality biological Monitoring Design of the present invention especially for this reason under situation a set of biological culture and sampling apparatus.Device can be seen in real time and look into record and affect flow velocity and the water temperature of growth of diatom algae, horizontal buoyancy aid firm under, under the cultivation of the diatom that grows nonparasitically upon another plant is in normal depth condition all the time.
Described buoyancy aid 7 is horizontal, adopts the hollow plastics of vinyl polymer, the bushing stainless steel junctor of surrounding, plastics aperture strict seal.
Described incubator 6, adopts netted cage, welds with horizontal buoyancy aid 7, and weld is arranged by five hole plum blossoms, guarantees to be connected firmly.
Described water temperature flow rates survey sensor 2, adopt compact spiral formula hydrometic propeller to carry sensor for measuring temperature, such sensor performance is stablized, energy life-time service; Supporting signals collecting and projector can realize automation collection metrical information.
Beneficial effect of the present invention is:
structure is simple, easily installs, convenient operation.
be highly suitable for great rivers, the river of the few stone of high concentration of sediment, uses in various lake.
device is stablized, and can adapt to the change of water-level fluctuation, presses close to the result of natural substrates.
Accompanying drawing explanation
Fig. 1 is the structural representation of apparatus of the present invention;
Fig. 2 is the structural map of incubator in culture apparatus;
Fig. 3 is the simple device composition schematic diagram for sample collecting.
Number in the figure: developing medium 1, integrated remotely monitored sensor 2, developing medium imports and exports 3, connecting device 4, monitored water body 5, cultivates boxes and baskets 6, buoyancy aid 7, sense line 8, signals collecting and projector 9, direction board 10, stainless steel wire rope 11, ground tackle 12, padlock 13, narrow-mouthed bottle 14, square plate 15, brush 16.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is further described, but protection scope of the present invention is not limited to described embodiment.
Embodiment 1: the cultivation and the collection that the invention provides a kind of periphyton culture and gathering device, particularly benthic diatom, comprises the natural culture apparatus 6 of the diatom that grows nonparasitically upon another plant, and water temperature flow rates measures collecting unit, diatom Sampling hold unit of growing nonparasitically upon another plant.
As shown in accompanying drawing 1 and Fig. 2, diatom culture apparatus of growing nonparasitically upon another plant comprises water floating body 7, and buoyancy aid 7 upper end bolt connects the billboard 10 of monitoring department, and billboard indicates purposes and setting unit, is damaged by ignorant person to prevent instrument; Growing nonparasitically upon another plant diatom incubator 6 in water floating body lower end, only welds a central part, weld position, four corners when four side welds connect when being welded on middle part.Incubator 6 adopts rectangular parallelepiped cage body structure, make hexagon and water body penetrating, the developing medium 1 of diatom is inserted on request and is cultivated in cage 6, temporally waits requirement to cultivate.Culture apparatus is provided with water temperature and flow velocity integrated intelligent remotely monitored sensor 2, sensor 2 is arranged on the central top of incubator, the transmission line 8 of sensor 2 is connected in buoyancy aid 7, buoyancy aid is provided with signals collecting and projector 9, wherein battery uses chargeable lithium cell, and buoyancy aid installing port will carry out seal approach.Affecting the water temperature of growth of diatom algae and flow velocity important information by radio telecontrol monitoring is wirelessly transmitted to Data centre.
For making diatom culture apparatus be fixed in the region of monitoring water body, connect culture apparatus and bank with stainless steel wire rope 11, wireline one end adopts padlock 13 to fasten buoyancy aid central authorities, and the other end connects ground tackle 12 by padlock 13 and is fixed on bank, to facilitate sampling.During diatom sampling, naturally the N number of hard stone 1 cultivating more than 1 month in cage 6 is taken out, with brush 16, the diatom brush of growing nonparasitically upon another plant of sunny slope in medium 1 is entered in porcelain dish 15, the surperficial diatom brush that faced south by medium 1 as far as possible when sampling enters in 15, and wash away totally with pure water, guarantee that brushing the diatom sample got all loads in bottle 14, the alcohol or the formaldehyde formulations that finally add final concentration 10% are preserved.
Embodiment 2: not needing the monitored area gathering water temperature and flow rate information, as Fig. 1 and Fig. 2, remove integrated remotely monitored sensor 2, sense line 8 and signals collecting and projector 9 during design, comparatively speaking, equipment is simpler, comprises the natural culture apparatus 6 of the diatom that grows nonparasitically upon another plant, diatom Sampling hold unit of growing nonparasitically upon another plant.
Now, diatom culture apparatus of growing nonparasitically upon another plant comprises water floating body 7, billboard 10, diatom incubator 6 of growing nonparasitically upon another plant, and buoyancy aid does not establish installation entrance, only has buoyancy aid sealing requirements all day.
Described water floating body 7 is rectangle structure, center of top erection instruction plate 10, and bottom bolts connects cage 6, can select to be provided with water temperature and flow velocity integrated intelligent remotely monitored sensor 2, be provided with signals collecting and projector 9 in corresponding buoyancy aid in cage 6;
Described cage is connected with buoyancy aid and can adopts bolt or be welded to connect, and cage and buoyancy aid length and width, 0.3 ~ 1.0 meter of scope, are 0.5 ~ 1.0 meter apart from buoyancy aid top at the bottom of cage.
Described developing medium 1 is placed in the cage 6 of six water flowings, it is characterized in that diatom easily in medium 1 surface growth, and matter is hard, and require not to be with silt particle without soft impurity, developing medium 1 preferentially adopts goose soft rock, and be generally 5 ~ 8 pieces, incubation time is advisable for more than 15 days.
3. as the collecting device in claim 1, it is characterized in that cage 6 upper surface establishes an opening, get the sunny slope of developing medium 1, brush 16 diatom brush of growing nonparasitically upon another plant is utilized to enter in plate 15, period can adopt watering can to spread the collection helping sunny slope diatom, finally the diatom gathered is injected volume 150 ~ 500ml narrow-mouthed bottle 14, add 1 ~ 2ml formaldehyde or alcohol reagent preservation.
The present invention is directed in existing sampling and be difficult to find qualified sampling media, medium is easily by technological deficiencies such as change of level affect, a kind of culture apparatus is devised specially for diatom biological survey and diatom water quality monitoring (WQM), device can stably float on water body, carries out cultivation and the collection of all kinds of periphyton simply and easily.
Claims (3)
1. the diatom that grows nonparasitically upon another plant is cultivated and a gathering device, by water floating body, and cage, developing medium, stainless steel wire rope, ground tackle, plate, brush, specimen bottle, and the composition such as formaldehyde formulations, is characterized in that:
Described floating (7) body waterborne is rectangle structure, center of top erection instruction plate (10), bottom bolts connects cage (6), can select to be provided with water temperature and flow velocity integrated intelligent remotely monitored sensor (2), be provided with signals collecting and projector (9) in corresponding buoyancy aid in cage (6);
Described cage is connected with buoyancy aid and can adopts bolt or be welded to connect, and cage and buoyancy aid length and width, 0.3 ~ 1.0 meter of scope, are 0.5 ~ 1.0 meter apart from buoyancy aid top at the bottom of cage.
2. developing medium as claimed in claim 1 is placed in the cage (6) of six water flowings, it is characterized in that diatom easily in medium (1) surface growth, matter is hard, require not to be with silt particle without soft impurity, developing medium (1) preferentially adopts goose soft rock, be generally 3 ~ 6 pieces, incubation time is advisable for more than 15 days.
3. as the collecting device in claim 1, it is characterized in that cage (6) upper surface establishes an opening, get the sunny slope of developing medium (1), brush (16) diatom brush of growing nonparasitically upon another plant is utilized to enter in plate (15), period can adopt watering can to spread the collection helping sunny slope diatom, finally the diatom gathered is injected volume 150 ~ 500ml narrow-mouthed bottle (14), add 1 ~ 2ml formaldehyde or alcohol reagent preservation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510384155.4A CN104962468A (en) | 2015-07-03 | 2015-07-03 | Epiphytic diatom culture and collection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510384155.4A CN104962468A (en) | 2015-07-03 | 2015-07-03 | Epiphytic diatom culture and collection device |
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| CN104962468A true CN104962468A (en) | 2015-10-07 |
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| CN201510384155.4A Pending CN104962468A (en) | 2015-07-03 | 2015-07-03 | Epiphytic diatom culture and collection device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838585A (en) * | 2016-06-01 | 2016-08-10 | 海南大学 | Device and method for cultivating diatom |
| CN108593338A (en) * | 2018-06-14 | 2018-09-28 | 西华师范大学 | A kind of multi-functional acquisition instrument of attached algae |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487808A (en) * | 2011-11-28 | 2012-06-13 | 暨南大学 | Method and special device for monitoring epiphytic algae in multiple-sediment river |
| CN202508263U (en) * | 2012-03-02 | 2012-10-31 | 珠海天岳科技有限公司 | Floating device and system |
| CN103175946A (en) * | 2013-03-04 | 2013-06-26 | 中国科学院南京地理与湖泊研究所 | System and method for automatically monitoring lake-flooding of shallow lake |
| CN203083840U (en) * | 2013-02-07 | 2013-07-24 | 陕西理工学院 | Sampling device for periphytic algae in still water |
| CN104663120A (en) * | 2015-03-27 | 2015-06-03 | 中国水产科学研究院黑龙江水产研究所 | Device and method for collecting field periphytic algae in open water stage |
| CN204824892U (en) * | 2015-07-03 | 2015-12-02 | 黎佛林 | Diatom that grows nonparasitically upon another plant is cultivateed and collection system |
-
2015
- 2015-07-03 CN CN201510384155.4A patent/CN104962468A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487808A (en) * | 2011-11-28 | 2012-06-13 | 暨南大学 | Method and special device for monitoring epiphytic algae in multiple-sediment river |
| CN202508263U (en) * | 2012-03-02 | 2012-10-31 | 珠海天岳科技有限公司 | Floating device and system |
| CN203083840U (en) * | 2013-02-07 | 2013-07-24 | 陕西理工学院 | Sampling device for periphytic algae in still water |
| CN103175946A (en) * | 2013-03-04 | 2013-06-26 | 中国科学院南京地理与湖泊研究所 | System and method for automatically monitoring lake-flooding of shallow lake |
| CN104663120A (en) * | 2015-03-27 | 2015-06-03 | 中国水产科学研究院黑龙江水产研究所 | Device and method for collecting field periphytic algae in open water stage |
| CN204824892U (en) * | 2015-07-03 | 2015-12-02 | 黎佛林 | Diatom that grows nonparasitically upon another plant is cultivateed and collection system |
Non-Patent Citations (1)
| Title |
|---|
| 易燃: "龙江底栖硅藻水质生物监测技术研究", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838585A (en) * | 2016-06-01 | 2016-08-10 | 海南大学 | Device and method for cultivating diatom |
| CN108593338A (en) * | 2018-06-14 | 2018-09-28 | 西华师范大学 | A kind of multi-functional acquisition instrument of attached algae |
| CN108593338B (en) * | 2018-06-14 | 2023-12-15 | 西华师范大学 | Multifunctional acquisition instrument for attached algae |
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Application publication date: 20151007 |
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