CN104961796A - Method for extracting rice bran protein from degreased rice bran - Google Patents

Abstract

The invention provides a method for extracting rice bran protein from degreased rice bran. The method comprises the following steps: 1) delivering the degreased rice bran into a refrigeration apparatus for refrigeration and carrying out refrigeration for 4 to 5 h in an environment with a temperature below 20 DEG C; 2) delivering the refrigerated degreased rice bran to a dryer and allowing the refrigerated degreased rice bran to enter a first temperature zone for drying; 3) allowing the degreased rice bran dried in the first temperature zone for to enter a second temperature zone; 4) pouring the dried degreased rice bran into a reaction vessel, introducing steam under stirring and carrying out heating and stirring for 30 to 60 min; 5) stopping heating of the reaction vessel, maintaining a temperature for 20 min, adding water 10 to 20 times of the degreased rice bran and soaking the degreased rice bran at 40 to 60 DEG C for 0.5 to 2 h; and 6) subjecting slurry obtained in the step 5) to ultrasonic extraction three times, wherein temperature for ultrasonic extraction is 40 to 50 DEG C, ultrasonic power is 500 W and each ultrasonic extraction lasts for 20 to 30 min, combining extracts, then carrying out filtering and concentration, adding 95% ethanol, carrying out standing for 24 h, removing supernatant through inclination, then carrying out pressure-reduced pumping filtration, heating an obtained filter cake with water until the filter cake is completely dissolved, then carrying out concentration, adding 95% ethanol and successively carrying out standing for 24 h, pumping filtration, vacuum drying at 50 DEG C and drying so as to obtain the rice bran protein.

Description

The method of rice bran protein is extracted from defatted rice bran

Technical field

The present invention relates to food technology field, be specifically related to a kind of method extracting rice bran protein from defatted rice bran.

Background technology

Rice bran is the pericarp, seed coat, perisperm, aleurone layer etc. of brown rice essence being smash the secondary rice generated when (essence grind) manufactures rice, and known oil content, protein, inorganics, vitamin B complex (particularly B1) are abundant.In addition, the gamma oryzanol also contained in known rice bran has antioxygenation, reduces the effect of cholesterol.Therefore, attempted rice bran processed goods to be used for nutritional fortification or applied as with the complementary goods acting as object, the food additives of enjoying them.And in production rice bran nutrients and rice bran nutritive fiber, rice bran stabilization is very important process procedure.

Rice bran protein is universally acknowledged quality plant albumen, research for many years shows, protein in rice bran is a kind of hypoallergenic albumen, its amino acid composition is close to the recommendation pattern of FAO/WH0, its nutritive value is far above the vegetable-protein such as corn, wheat, can match in excellence or beauty with the high-quality such as egg, milk animal proteinum, be a kind of novel plant albumen of great exploitation potential for its.According to rough reckoning, China can obtain about 2,000 ten thousand tons, rice bran every year, is a kind of renewable resources had a large capacity and a wide range, if protein wherein all can be extracted, can obtain the quality plant albumen of about 2,000,000 tons every year.Therefore, from rice bran, seek new protein resource, increase gradually for alleviation China population, the present situation that protein resource is day by day in short supply has important practical significance.

Summary of the invention

It is simple that technical problem to be solved by this invention is to provide a kind of technique, the method extracting rice bran protein from defatted rice bran that yield is high.

Technical problem to be solved by this invention realizes by the following technical solutions:

From defatted rice bran, extract a method for rice bran protein, step is as follows:

1) defatted rice bran is sent into refrigerating apparatus and carry out freezing treatment, freezing 4-5 hour under subzero 20 DEG C of environment; Make defatted rice bran stabilization through freezing treatment, facilitate subsequent disposal;

2) by step 1) in freezing after defatted rice bran send in dryer, enter the first humidity province and dry, the temperature of the first humidity province is incremented to 80 DEG C gradually from 70 DEG C, and time controling is at 1-3 minute, and speed is 0.2-0.5 meter per second;

3) by step 2) in first humidity province dry after defatted rice bran enter the second humidity province, the temperature of the second humidity province is decremented to 70 DEG C gradually from 80 DEG C, and time controling was at 4 minutes, and speed is 0.2-0.5 meter per second;

4) by step 3) in the defatted rice bran of drying pour in reactor and inject steam while stirring, under vapour temperature in reactor reaches the condition of 130 DEG C, allow defatted rice bran fully be stirred in a kettle., make it be heated evenly, heated and stirred 30-60 minute;

5) to step 4) in reactor stop heating, be incubated 20 minutes, add water 10-20 times amount, in 40-60 DEG C immersion 0.5-2 hour;

6) by step 5) in slurry in temperature 40 DEG C-50 DEG C, ultrasonic power is 500W, supersound extraction 3 times, each 20-30 minute, united extraction liquid, filters, concentrated, add 95% ethanol, leave standstill 24 hours, incline supernatant liquor, decompress filter, and filter cake is heated to dissolve completely with water again, concentrated, add 95% ethanol, leave standstill 24 hours, suction filtration, 50 DEG C of vacuum-dryings, dry and obtain rice bran protein.

The invention has the beneficial effects as follows: extraction time of the present invention is short, and energy consumption is low, feed throughput is large, at double or several times improve, and impurity is few, and effective constituent is easy to be separated, purification, and cost is low, and overall economic efficiency is remarkable.

Embodiment

The technique means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.

Embodiment 1

From defatted rice bran, extract a method for rice bran protein, step is as follows:

1) defatted rice bran is sent into refrigerating apparatus and carry out freezing treatment, freezing 4-5 hour under subzero 20 DEG C of environment; Make defatted rice bran stabilization through freezing treatment, facilitate subsequent disposal;

2) by step 1) in freezing after defatted rice bran send in dryer, enter the first humidity province and dry, the temperature of the first humidity province is incremented to 80 DEG C gradually from 70 DEG C, and time controling is at 1-3 minute, and speed is 0.2-0.5 meter per second;

3) by step 2) in first humidity province dry after defatted rice bran enter the second humidity province, the temperature of the second humidity province is decremented to 70 DEG C gradually from 80 DEG C, and time controling was at 4 minutes, and speed is 0.2-0.5 meter per second;

4) by step 3) in the defatted rice bran of drying pour in reactor and inject steam while stirring, under vapour temperature in reactor reaches the condition of 130 DEG C, allow defatted rice bran fully be stirred in a kettle., make it be heated evenly, heated and stirred 30-60 minute;

5) to step 4) in reactor stop heating, be incubated 20 minutes, add water 10-20 times amount, in 40-60 DEG C immersion 0.5-2 hour;

6) by step 5) in slurry in temperature 40 DEG C-50 DEG C, ultrasonic power is 500W, supersound extraction 3 times, each 20-30 minute, united extraction liquid, filters, concentrated, add 95% ethanol, leave standstill 24 hours, incline supernatant liquor, decompress filter, and filter cake is heated to dissolve completely with water again, concentrated, add 95% ethanol, leave standstill 24 hours, suction filtration, 50 DEG C of vacuum-dryings, dry and obtain rice bran protein.

More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.

Claims (1)

1. from defatted rice bran, extract a method for rice bran protein, it is characterized in that, step is as follows:

1) defatted rice bran is sent into refrigerating apparatus and carry out freezing treatment, freezing 4-5 hour under subzero 20 DEG C of environment;

2) by step 1) in freezing after defatted rice bran send in dryer, enter the first humidity province and dry, the temperature of the first humidity province is incremented to 80 DEG C gradually from 70 DEG C, and time controling is at 1-3 minute, and speed is 0.2-0.5 meter per second;

3) by step 2) in first humidity province dry after defatted rice bran enter the second humidity province, the temperature of the second humidity province is decremented to 70 DEG C gradually from 80 DEG C, and time controling was at 4 minutes, and speed is 0.2-0.5 meter per second;

4) by step 3) in the defatted rice bran of drying pour in reactor and inject steam while stirring, under vapour temperature in reactor reaches the condition of 130 DEG C, allow defatted rice bran fully be stirred in a kettle., make it be heated evenly, heated and stirred 30-60 minute;

5) to step 4) in reactor stop heating, be incubated 20 minutes, add water 10-20 times amount, in 40-60 DEG C immersion 0.5-2 hour;

6) by step 5) in slurry in temperature 40 DEG C-50 DEG C, ultrasonic power is 500W, supersound extraction 3 times, each 20-30 minute, united extraction liquid, filters, concentrated, add 95% ethanol, leave standstill 24 hours, incline supernatant liquor, decompress filter, and filter cake is heated to dissolve completely with water again, concentrated, add 95% ethanol, leave standstill 24 hours, suction filtration, 50 DEG C of vacuum-dryings, dry and obtain rice bran protein.