CN104931560A - Biosensor and preparation method and application thereof - Google Patents
Biosensor and preparation method and application thereof Download PDFInfo
- Publication number
- CN104931560A CN104931560A CN201510424013.6A CN201510424013A CN104931560A CN 104931560 A CN104931560 A CN 104931560A CN 201510424013 A CN201510424013 A CN 201510424013A CN 104931560 A CN104931560 A CN 104931560A
- Authority
- CN
- China
- Prior art keywords
- layer
- electrode
- agglutinin
- reagent
- base material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to the field of biological detection instruments, in particular to a biosensor. The biosensor comprises a reagent layer. The reagent layer contains red blood cell agglutinant or an omentum layer adhering to the reagent layer. The omentum layer is treated through the red blood cell agglutinant. The invention further provides a preparation method and application of the biosensor. As the problem of the sensibility of the hematocrit value is not solved completely in the prior art, the problem of the sensibility of the hematocrit value is solved by reducing the sensibility of the hematocrit value; accordingly, the accuracy of testing results is improved, and interference of red blood cells on the testing results is reduced to the maximum extent.
Description
Technical field
The present invention relates to biological detection instrument field, be specifically related to a kind of biology sensor and its preparation method and application.
Background technology
Biology sensor (biosensor) is a kind of responsive and its concentration is converted to the instrument that electric signal detects to biological substance.Make analysis tool that recognition component (comprising the bioactivators such as enzyme, antibody, antigen, microorganism, cell, tissue, nucleic acid), suitable physics and chemistry transducer (as oxygen electrode, photosensitive tube, field effect transistor, piezoelectric crystal etc.) and signal amplifying apparatus form or system by immobilized biological sensitive materials.
Electrochemica biological sensor has been widely used in in-vitro diagnosis field, these instrument systems can detect the physiologically substance in body fluid, facilitate doctor to patient status analysis and diagnosis, also have some instrument systems to be applied to family to use, facilitate the self-monitoring of patient, these physical signs comprise blood sugar, cholesterol, uric acid, triglyceride, lactic acid, ketoboidies, enzyme etc.Current biology sensor has been widely used in clinical detection, and wherein the most universal example is portable blood sugar test macro.
Hematocrit value (Hematocrit), also known as packed cell volume (Hct), refer to that a certain amount of anticoagulated whole blood is after centrifugation, the red blood cell recording sinking accounts for the volumetric ratio of whole blood, is the straightforward procedure of a kind of indirect reflection erythrocyte number size and volume.Hematocrit value normal reference value is as follows: man 0.40 ~ 0.50; Female 0.35 ~ 0.45.Clinically, due to different physiology, pathological state, hematocrit value can increase or reduce.As gestation, anaemia or treatment, hematocrit value can reduce, and some extreme case even can lower than 20%; Neonatal packed cell volume can be higher, usually can to 50% ~ 65%, and the infant of some polycythemia even can to 70%.
Relatively more conventional detection body fluid is blood and urine, for blood, due to sex, physiological status, the pathological state difference of individual, hematocrit value may have larger difference, and therefore hematocrit value is the major reason affecting electrochemica biological sensor accuracy in detection.
The method that current electrochemica biological sensor solves hematocrit value impact mainly contains, reflection district adds one deck porous nethike embrane, or in reagent system, add the polymer that can form grid structure, red blood cell filtration is fallen, avoid them to enter conversion zone disturbance reponse.As: the United States Patent (USP) (Disposable glucose test strip and method and compositions for making same) that publication date is on 09 14th, 1999, patent publication No. is US5951836 (A), by adding silicon dioxide in reagent, hydrophilic and hydrophobic two-dimensional network structure is formed after dry, stop red blood cell to enter electrode by this reticulate texture, thus reduce the susceptibility of test to hematocrit value.And for example: the United States Patent (USP) (Gel formation to reduce hematocrit sensitivity in electrichemical test) that publication date is on 06 26th, 2008, publication number is US2008/0149480A1, by adding gel-type vehicle in reagent, can filtering red blood cell, stop or reduce a part of red blood cell and enter contact electrode, thus reduce the susceptibility of electrochemica biological sensor test to hematocrit value.And for example: publication date is on Dec 03rd, 2009, publication number is the United States Patent (USP) (Use of Alginate to Reduce Hematocrit Bias in Biosensors) of US20090294302A1, by adding alginate polymer in reagent, reduce erythrocytic migration, stop or reduce a part of red blood cell and enter contact electrode, thus reduce the susceptibility of electrochemica biological sensor test to hematocrit value.And for example: the United States Patent (USP) (Biosensor having improved hematocrit and oxygen biases) that publication date is on March 10th, 2009, publication number is US7501053 (B2), mention and pass through membrane technology, red blood cell filtration is fallen, thus reduces the susceptibility of electrochemica biological sensor test to hematocrit value.
But the method for these patent literatures above-mentioned does not thoroughly solve the problem of hematocrit value susceptibility, these technological means still have some red blood cells can pass the micropore of film or the grid structure of polymer, enter reaction zone and contact electrode, thus disturbance reponse and test; More have some personnel or sample, erythrocytic size is smaller, is more prone to the grid structure of micropore through film or polymer, have impact on test result greatly.
Summary of the invention
The problems referred to above existed for prior art and defect, the present invention aims to provide a kind of biology sensor, from reduction hematocrit value susceptibility, to the problem of hematocrit value susceptibility can be improved, thus improve the accuracy of test result, at utmost reduce red blood cell to the interference of test result.
For realizing object of the present invention, inventor provides following technical scheme:
The present invention provide firstly the first biology sensor, its composition comprises insulated base material layer, be attached to the electrode system in insulated base material layer, described electrode system at least comprises a working electrode and one to electrode, cover the reagent layer above electrode system, described reagent layer at least should cover a working electrode, and the reaction chamber be positioned at above electrode system and reagent layer, described reaction chamber is made up of space layer and overlayer, and reaction chamber has bleeder vent, wherein: the component of described reagent layer contains red cell agglutination agent.
Present invention also offers the second biology sensor, its composition comprises insulated base material layer, be attached to the electrode system in insulated base material layer, described electrode system at least comprises a working electrode and one to electrode, cover the reagent layer above electrode system, described reagent layer at least should cover a working electrode, adhere to the nethike embrane layer on reagent layer, and the reaction chamber be positioned at above electrode system and nethike embrane layer, described reaction chamber is made up of space layer and overlayer, and reaction chamber has bleeder vent, wherein: described nethike embrane layer is the nethike embrane layer of red cell agglutination agent process.
As preferably, in biology sensor of the present invention, described red cell agglutination agent is agglutinin or anti erythrocyte antibody, and agglutinin comprises animal origin agglutinin and phytolectin.Phytolectin ratio is easier to obtain, price is also more cheap, optional phytolectin includes but not limited to concanavalin A (Conconvalina, ConA), wheat germ element (Wheat germ agglutinin, WGA), peanut agglutinin (Peanut agglutinin, PNA) and soybean agglutinin (Soybean agglutinin, SBA), working concentration interval in reagent solution is 0.01% ~ 5.0%, and preferred working concentration interval is 0.1% ~ 1.0%.Anti erythrocyte antibody comprises monoclonal antibody and polyclonal antibody, preferably can make the polyclonal antibody of different blood group red cell agglutination, and the working concentration interval in reagent solution is 1ug/mL ~ 10mg/mL, and preferred working concentration interval is 10ug/mL ~ 0.1mg/mL.
As preferably, in biology sensor of the present invention, each electrode in described electrode system has extension wire and contact region, and extension wire can be identical with electrode material with the material of contact region, also can be different; Be connected with tester by contact region, tester can provide and receive the reaction information of biology sensor, and the original signal of reaction information is changed into test result by computing.
As preferably, in biology sensor of the present invention, the thickness of described space layer is 0.025mm ~ 0.5mm.More preferably the thickness of space layer is 0.075mm ~ 0.125mm.Space layer, in reaction chamber region hollow out, defines reaction chamber in conjunction with overlayer.The material of space layer can be band base material or the adhesive tape not with base material, processes rear bonding and gets on; Also can be glue or polymer paste, be got on by serigraphy; As electrode separates without other insulating material, the material of space layer must be good insulating material.
As preferably, in biology sensor of the present invention, described overlayer can use PET material, the material that the hydrophilic treatment of preferably clear is crossed, transparently user can be helped easily to confirm reaction zone sample introduction state, the hydrophilic sample introduction that can make is more smooth and easy, and non-reaction chamber region can be opaque, printing sample introduction mark and Logo, available material is as 9971 hydrophilic film of 3M company.Reaction chamber should have bleeder vent, to form syphonic effect, the bleeder vent of the embodiment of the present invention is realized by the space layer of hollow out reaction chamber.
Reaction chamber is built by base material, space layer and overlayer and forms, and reaction chamber comprises the electrode on base material and the reagent on electrode.
As preferably, in biology sensor of the present invention, the material of insulated base material layer includes but not limited to the one in PET, PP, PVC, acrylic.
As preferably, in biology sensor of the present invention, the material of electrode includes but not limited to the one in graphite, silver, gold, platinum, palladium, ITO, IZO.
Reagent layer of the present invention comprises the components such as enzyme, electron mediator, damping fluid, surfactant, polymer.The effect of enzyme is specific and detected material reaction; Electron mediator obtains electronics from the reaction of enzyme and detected material, and is diffused on electrode and discharges electronics, and forms signal, and this signal can be caught by tested instrument; Damping fluid ensures a suitable reaction environment; Surfactant can Optimal reaction conditions, and promotes that sample spreads; Polymer can form grid structure, and immobilized enzyme to a certain extent.
According to the difference of test item, different enzymes can being selected, glucose dehydrogenase, glucose oxidase can be selected as detected blood sugar; Detect lactic acid, Lactate Oxidase, lactic dehydrogenase can be selected; Detect uric acid, can urate oxidase be selected, also can select without enzyme without enzymuria acid sensor; Detect beta-hydroxybutyric acid, can beta-hydroxybutyric dehydrogenase etc. be used; Separately some needs the parameter of two or more enzymes, as cholesterol detection, needs the combination of cholesterol esterase and cholesterol oxidase or cholesterin dehydrogenase; The combination of some oxidase and peroxidase detects etc.
Representational electron mediator has ferrocene derivatives, as ferrocenecarboxylic acid; The ferricyanide, as the potassium ferricyanide; Ruthenium complex, as six ammonia ruthenium trichlorides; Prussian blue; Methyl blue; Mai Er Doran; Toluphenazine Methylsulfate salt compounds; 2,6-dichloroindophenol; Quinones; Cobalt, nickel complex; Four nitre azole compounds; Naphthols chlorine; 4-AA etc.
Polymer comprises bonding agent and filling agent etc.Bonding agent can be xanthans, gelatin, hydroxyethyl cellulose, hydroxymethyl-propyl cellulose etc.; Filling agent can be cellulose family, as microcrystalline cellulose, methylcellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxymethyl-propyl cellulose, sodium carboxymethyl cellulose, sodium carboxymethyl starch, apatite, gasification silicon dioxide, porcelain earth, smectite, shitosan, cyclodextrin, sodium alginate, glucosides, PMMA, agar, gelatin, polyglycol, polyvinylpyrrolidone, PEO, PVA, PVP/VA etc.These polymers can be formed and better can form good grid structure, filtering red blood cell and immobilized enzyme are all had certain effect, wherein sodium carboxymethyl cellulose, hydroxymethyl-propyl cellulose sodium and sodium alginate filtering red blood cell better effects if, and do not affect the diffusion of reactive material.
Alternative surfactant has: Triton X-100 class, as Triton X-100; Polyoxyethylene 20 sorbitan monolaurate, as Tween 20; Chlolic acid derivatives class, as 3-[(3-courage amidopropyl) dimethylammonio]-1-propane sulfonic acid salt, NaTDC; Brij-35, as Brij 35; Quaternary ammonium salt, as cetyl trimethyl ammonium bromide; Glucosides class, as dodecyl-β-D-Maltose glycosides; Poly-(oxo-1,2-second dimethylene)-α-nonyl phenyl-ω-hydroxy kind, as TERGITOL (TM) NP-40, etc.
Alternative damping fluid is also a lot, and due to activity and the stable region restriction of enzyme, the pH value buffering range of selection is usually between 3 ~ 11.Typical damping fluid has, acetate buffer, citrate buffer solution, phosphate buffer, imidazole buffer, borate buffer solution, TES, BES, HEPES, PIPES, MOPS, Bicine, Tris, CHES, MES, ADA, CAPS, EPPS, POPSO, CAPS etc.
In the present invention, described red cell agglutination agent can be prepared together with above-mentioned reaction reagent (comprising the components such as enzyme, electron mediator, damping fluid, surfactant and polymer), and is fixed to together on electrode as reagent layer; Red cell agglutination agent also can be mixed with solution separately in conjunction with other reagent (comprising the component such as surfactant, polymer), then with this solution-treated porous nethike embrane, after drying, this nethike embrane adheres on reagent layer, and nethike embrane can select the polyester nethike embrane of Sefar AG company.Also can above-mentioned two kinds of methods combining together.
Present invention also offers the preparation method of the first above-mentioned biology sensor, comprising:
(1) in insulated base material layer, electrode system is formed, described electrode system at least comprises a working electrode and one to electrode, job operation has: by serigraphy, specific electrode pattern is printed onto in insulated base material layer, then by heating or infrared or ultraviolet is dry; Or electrode material is attached in insulated base material layer by the mode of sputtering, then processes specific electrode pattern by the mode of laser ablation,
(2) reagent layer (reagent layer component comprises enzyme, electron mediator, damping fluid, surfactant, polymer and red cell agglutination agent) the method processing such as to be extruded by some liquid, serigraphy, inkjet printing, crack and is fixed on electrode,
(3) space layer of reaction chamber of fitting on electrode system and reagent layer and overlayer, complete, wherein: with base material or the adhesive tape not with base material for space layer material, be bonded on electrode system and reagent layer after processing; Or with glue or polymer paste for space layer material, by serigraphy on electrode system and reagent layer.
Present invention also offers the preparation method of above-mentioned the second biology sensor, comprising:
(1) in insulated base material layer, electrode system is formed, described electrode system at least comprises a working electrode and one to electrode, job operation has: by serigraphy, specific electrode pattern is printed onto in insulated base material layer, then by heating or infrared or ultraviolet is dry; Or electrode material is attached in insulated base material layer by the mode of sputtering, then processes specific electrode pattern by the mode of laser ablation,
(2) reagent layer (reagent layer component comprises enzyme, electron mediator, damping fluid, surfactant and polymer) the method processing such as to be extruded by some liquid, serigraphy, inkjet printing, crack and is fixed on electrode,
(3) with containing red cell agglutination agent solution process porous nethike embrane, after drying, this nethike embrane layer is adhered on reagent layer,
(4) space layer of reaction chamber of fitting on electrode system and nethike embrane layer and overlayer, complete, wherein: with base material or the adhesive tape not with base material for space layer material, be bonded on electrode system and nethike embrane layer after processing; Or with glue or polymer paste for space layer material, by serigraphy on electrode system and nethike embrane layer.
As preferably, in preparation method of the present invention, described red cell agglutination agent is agglutinin or anti erythrocyte antibody, and agglutinin comprises animal origin agglutinin and phytolectin.Phytolectin ratio is easier to obtain, price is also more cheap, optional phytolectin includes but not limited to concanavalin A (Conconvalina, ConA), wheat germ element (Wheat germ agglutinin, WGA), peanut agglutinin (Peanut agglutinin, PNA) and soybean agglutinin (Soybean agglutinin, SBA), working concentration interval in reagent solution is 0.01% ~ 5.0%, and preferred working concentration interval is 0.1% ~ 1.0%.Anti erythrocyte antibody comprises monoclonal antibody and polyclonal antibody, preferably can make the polyclonal antibody of different blood group red cell agglutination, and the working concentration interval in reagent solution is 1ug/mL ~ 10mg/mL, and preferred working concentration interval is 10ug/mL ~ 0.1mg/mL.
Present invention also offers the application of a kind of biology sensor in blood testing, wherein: described biology sensor composition comprises the immobilized biological sensitive materials of employing and makes recognition component, and biological sensitive materials is containing red cell agglutination agent.
As preferably, in the application of a kind of biology sensor of the present invention in blood testing: described red cell agglutination agent is agglutinin or anti erythrocyte antibody, and agglutinin comprises animal origin agglutinin and phytolectin.Phytolectin ratio is easier to obtain, price is also more cheap, optional phytolectin includes but not limited to concanavalin A (Conconvalina, ConA), wheat germ element (Wheat germ agglutinin, WGA), peanut agglutinin (Peanut agglutinin, PNA) and soybean agglutinin (Soybean agglutinin, SBA), working concentration interval in reagent solution is 0.01% ~ 5.0%, and preferred working concentration interval is 0.1% ~ 1.0%.Anti erythrocyte antibody comprises monoclonal antibody and polyclonal antibody, preferably can make the polyclonal antibody of different blood group red cell agglutination, and the working concentration interval in reagent solution is 1ug/mL ~ 10mg/mL, and preferred working concentration interval is 10ug/mL ~ 0.1mg/mL.
As preferably, in the application of a kind of biology sensor of the present invention in blood testing, detection method operates in the steps below:
(1) sample of blood, drawn (sample can be capillary blood, venous blood, arterial blood);
(2) biology sensor is connected detecting instrument by contact element;
(3) detecting instrument provides detection signal, adds potential difference (PD), and monitor sample introduction signal as on Different electrodes on a biosensor;
(4) blood sample is added to biology sensor adding mouth, sample automatic siphon is to reaction zone;
(5), after detecting instrument detects sample introduction signal, require to start to gather reaction signal according to test logic;
(6) detecting instrument is according to the signal collected, the working curve made in advance, and reaction signal is converted into result.
Present invention also offers the application of red cell agglutination agent in a kind of reagent composition reducing hematocrit value susceptibility, wherein, when described red cell agglutination agent is agglutinin, in reagent composition, working concentration interval is 0.01% ~ 5.0%, preferably 0.1% ~ 1.0%; When described red cell agglutination agent is anti erythrocyte antibody, in reagent composition, working concentration interval is 1ug/mL ~ 10mg/mL, and preferred working concentration interval is 10ug/mL ~ 0.1mg/mL.The composition reducing the reagent composition of hematocrit value susceptibility comprises enzyme and (can not contain, as uric acid detect in can not need to add enzyme), electron mediator, damping fluid, surfactant, at least one can form polymer and the red cell agglutination agent of rack or micropore, or the polymer that composition comprises surfactant, at least one can form rack or micropore and red cell agglutination agent.The proportioning of other various materials except red cell agglutination agent, selects according to this area routine according to actual needs, repeats no longer one by one herein.
The effect of enzyme is specific and detected material reaction; Electron mediator obtains electronics from the reaction of enzyme and detected material, and is diffused on electrode and discharges electronics, and forms signal, and this signal can be caught by tested instrument; Damping fluid ensures a suitable reaction environment; Surfactant can Optimal reaction conditions, and promotes that sample spreads; Polymer can form grid structure, and immobilized enzyme to a certain extent.Prepare together with the components such as red cell agglutination agent, enzyme, electron mediator, damping fluid, surfactant and polymer, and be fixed to together on electrode as reagent layer.
Described red cell agglutination agent is agglutinin or anti erythrocyte antibody, and agglutinin comprises animal origin agglutinin and phytolectin.Phytolectin ratio is easier to obtain, price is also more cheap, optional phytolectin includes but not limited to concanavalin A (Conconvalina, ConA), wheat germ element (Wheat germ agglutinin, WGA), peanut agglutinin (Peanut agglutinin, PNA) and soybean agglutinin (Soybean agglutinin, SBA), working concentration interval in reagent solution is 0.01% ~ 5.0%, and preferred working concentration interval is 0.1% ~ 1.0%.Anti erythrocyte antibody comprises monoclonal antibody and polyclonal antibody, preferably can make the polyclonal antibody of different blood group red cell agglutination, and the working concentration interval in reagent solution is 1ug/mL ~ 10mg/mL, and preferred working concentration interval is 10ug/mL ~ 0.1mg/mL.
According to the difference of test item, different enzymes can being selected, glucose dehydrogenase, glucose oxidase can be selected as detected blood sugar; Detect lactic acid, Lactate Oxidase, lactic dehydrogenase can be selected; Detect uric acid, can urate oxidase be selected, also can select without enzyme without enzymuria acid sensor; Detect beta-hydroxybutyric acid, can beta-hydroxybutyric dehydrogenase etc. be used; Separately some needs the parameter of two or more enzymes, as cholesterol detection, needs the combination of cholesterol esterase and cholesterol oxidase or cholesterin dehydrogenase; The combination of some oxidase and peroxidase detects etc.
Representational electron mediator has ferrocene derivatives, as ferrocenecarboxylic acid; The ferricyanide, as the potassium ferricyanide; Ruthenium complex, as six ammonia ruthenium trichlorides; Prussian blue; Methyl blue; Mai Er Doran; Toluphenazine Methylsulfate salt compounds; 2,6-dichloroindophenol; Quinones; Cobalt, nickel complex; Four nitre azole compounds; Naphthols chlorine; 4-AA etc.
The polymer that at least one can form rack or micropore comprises bonding agent and filling agent etc.Bonding agent can be xanthans, gelatin, hydroxyethyl cellulose, hydroxymethyl-propyl cellulose etc.; Filling agent can be cellulose family, as microcrystalline cellulose, methylcellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxymethyl-propyl cellulose, sodium carboxymethyl cellulose, sodium carboxymethyl starch, apatite, gasification silicon dioxide, porcelain earth, smectite, shitosan, cyclodextrin, sodium alginate, glucosides, PMMA, agar, gelatin, polyglycol, polyvinylpyrrolidone, PEO, PVA, PVP/VA etc.These polymers can be formed and better can form good grid structure, filtering red blood cell and immobilized enzyme are all had certain effect, wherein sodium carboxymethyl cellulose, hydroxymethyl-propyl cellulose sodium and sodium alginate filtering red blood cell better effects if, and do not affect the diffusion of reactive material.
Alternative surfactant has: Triton X-100 class, as Triton X-100; Polyoxyethylene 20 sorbitan monolaurate, as Tween 20; Chlolic acid derivatives class, as 3-[(3-courage amidopropyl) dimethylammonio]-1-propane sulfonic acid salt, NaTDC; Brij-35, as Brij 35; Quaternary ammonium salt, as cetyl trimethyl ammonium bromide; Glucosides class, as dodecyl-β-D-Maltose glycosides; Poly-(oxo-1,2-second dimethylene)-α-nonyl phenyl-ω-hydroxy kind, as TERGITOL (TM) NP-40, etc.
Alternative damping fluid is also a lot, and due to activity and the stable region restriction of enzyme, the pH value buffering range of selection is usually between 3 ~ 11.Typical damping fluid has, acetate buffer, citrate buffer solution, phosphate buffer, imidazole buffer, borate buffer solution, TES, BES, HEPES, PIPES, MOPS, Bicine, Tris, CHES, MES, ADA, CAPS, EPPS, POPSO, CAPS etc.
Compared with prior art, the present invention has the following advantages:
The present invention introduces red cell agglutination agent in reagent component, after blood sample adds, red cell agglutination agent impels red cell agglutination, make it to form the larger agglomerates of size, not easily pass through micropore or the grid structure of film or polymer formation, effectively filtered red blood cell, avoid red blood cell to enter reaction zone and and electrode contact, thus achieve and reduce biosensor test to the susceptibility of hematocrit value.
Experimental results shows, what use without agglutinin in the deviation ratio reagent of the hematocrit value sample test result of biology sensor of the present invention is less, illustrates that the reduction hematocrit value susceptibility of the present invention to sensor has actual effect.
Accompanying drawing explanation
Figure 1A and Figure 1B is STRUCTURE DECOMPOSITION schematic diagram and the structural integrity schematic diagram of a kind of biology sensor of the present invention.
Fig. 2 A and Fig. 2 B is STRUCTURE DECOMPOSITION schematic diagram and the structural integrity schematic diagram of the another kind of biology sensor of the present invention.
Fig. 3 is the deviation comparison diagram (blood sugar concentration 150 mg/dL) (in figure, example refers to embodiment, lower same) that the present invention contains the biology sensor of two kinds of structures of phytolectin and the height two ends hematocrit value sample test result of comparative example and 40% hematocrit value sample test result.
Fig. 4 is the deviation comparison diagram (blood sugar concentration 400 mg/dL) that the present invention contains the biology sensor of two kinds of structures of phytolectin and the height two ends hematocrit value sample test result of comparative example and 40% hematocrit value sample test result.
Fig. 5 is the deviation comparison diagram (blood sugar concentration 150 mg/dL) that the present invention contains the biology sensor of two kinds of structures of anti-human erythrocyte antibody and the height two ends hematocrit value sample test result of comparative example and 40% hematocrit value sample test result.
Fig. 6 is deviation comparison diagram (blood sugar concentration 400 mg/dL that the present invention contains the biology sensor of two kinds of structures of anti-human erythrocyte antibody and the height two ends hematocrit value sample test result of comparative example and 40% hematocrit value sample test result.
Fig. 7 is the deviation comparison diagram (blood sugar concentration 150 mg/dL) of the biology sensor of the first structure of another content phytolectin of the present invention and the height two ends hematocrit value sample test result of comparative example and 40% hematocrit value sample test result.
Fig. 8 is the deviation comparison diagram (blood sugar concentration 400 mg/dL) of the biology sensor of the first structure of another content phytolectin of the present invention and the height two ends hematocrit value sample test result of comparative example and 40% hematocrit value sample test result.
Fig. 9 is the deviation comparison diagram (blood sugar concentration 150 mg/dL) of the biology sensor of the first structure of another kind phytolectin of the present invention and the height two ends hematocrit value sample test result of comparative example and 40% hematocrit value sample test result.
Figure 10 is the deviation comparison diagram (blood sugar concentration 400 mg/dL) of the biology sensor of the first structure of another kind phytolectin of the present invention and the height two ends hematocrit value sample test result of comparative example and 40% hematocrit value sample test result.
Embodiment
Below in conjunction with embodiment, further illustrate content of the present invention.Should be appreciated that enforcement of the present invention is not limited to the following examples, any pro forma accommodation make the present invention and/or change all will fall into scope.
In the present invention, if not refer in particular to, all equipment and raw material etc. all can be buied from market or the industry is conventional.Method in following embodiment, if no special instructions, is the conventional method of this area.
embodiment 1
As shown in FIG. 1A and 1B, a kind of biology sensor, composition comprises insulated base material layer, insulating substrate is PET, be attached to the electrode system in insulated base material layer, described electrode system comprises a working electrode and one to electrode, electrode material is graphite, each electrode in described electrode system has extension wire and contact region, be connected with tester by contact region, cover the reagent layer above electrode system, described reagent layer covers working electrode, and the reaction chamber be positioned at above electrode system and reagent layer, described reaction chamber is made up of space layer and overlayer, the thickness of space layer is 0.125mm, overlayer adopts 9971 hydrophilic film of 3M company, and reaction chamber has bleeder vent, so that entering of sample, wherein: the component of described reagent layer contains red cell agglutination agent-agglutinin,
Containing the reagent solution of agglutinin, composed as follows:
Phosphate dihydrate disodium hydrogen: 1.1395g
Sodium dihydrogen phosphate dihydrate: 2.1223g
Sodium carboxymethyl cellulose (Sigma C5678): 1.0000g
Gelatin (Sigma G7041): 0.0500g
Triton X-100：0.2000g
Polyglycol (Sigma 95904): 0.5000g
The potassium ferricyanide: 3.2900g
Glucose oxidase (Amano, GO-AM): 600KU
Lectin (Kidney bean) (Sigma L8754): 0.1000g
Purified water: 92.7000g,
Reagent solution containing agglutinin passes through to stir, and makes it abundant dissolving, uniform composition, for subsequent use.
Biology sensor preparation method is as follows:
(1) by serigraphy, specific electrode pattern is printed onto in insulated base material layer, then heat drying, electrode system is attached in insulated base material layer,
(2) reagent solution containing agglutinin after stirring is assigned on the graphite electrode of serigraphy making by some liquid mode, and then dry 15 minutes at temperature 55 DEG C, reagent layer is fixedly worked on electrode,
(3) space layer of reaction chamber of fitting on electrode system and reagent layer and overlayer, complete, wherein: with the adhesive tape with base material for space layer material, be bonded on electrode system and reagent layer after processing.
embodiment 2
As shown in Figure 2 A and 2 B, a kind of biology sensor, composition comprises insulated base material layer, insulating substrate is acrylic, be attached to the electrode system in insulated base material layer, described electrode system comprises a working electrode and one to electrode, electrode material is graphite, each electrode in described electrode system has extension wire and contact region, be connected with tester by contact region, cover the reagent layer above electrode system, described reagent layer covers working electrode, adhere to the nethike embrane layer on reagent layer, and the reaction chamber be positioned at above electrode system and reagent layer, described reaction chamber is made up of space layer and overlayer, the thickness of space layer is 0.125mm, overlayer adopts 9971 hydrophilic film of 3M company, and reaction chamber has bleeder vent, so that entering of sample, wherein: described nethike embrane layer is the nethike embrane layer that red cell agglutination agent (i.e. agglutinin) processes,
Not containing the reagent solution of agglutinin, composed as follows: phosphate dihydrate disodium hydrogen: 1.1395g, sodium dihydrogen phosphate dihydrate: 2.1223g, sodium carboxymethyl cellulose (Sigma C5678): 1.0000g, gelatin (Sigma G7041): 0.0500g, Triton X-100:0.2000g, polyglycol (Sigma 95904): 0.5000g, the potassium ferricyanide: 3.2900g, glucose oxidase (Amano, GO-AM): 600KU and purified water: 92.7000g, reagent solution not containing agglutinin passes through to stir, make it abundant dissolving, uniform composition, for subsequent use;
Containing the reagent solution of the process nethike embrane of agglutinin, composed as follows: sodium carboxymethyl cellulose (Sigma C5678): 1.0000g, Triton X-100:0.2000g, lectin (Kidney bean) (Sigma L8754): 0.1000g and purified water: 98.7000g, reagent solution containing the process nethike embrane of agglutinin passes through to stir, make it abundant dissolving, uniform composition, for subsequent use
Biology sensor preparation method is as follows:
(1) by serigraphy, specific electrode pattern is printed onto in insulated base material layer, then heat drying, electrode system is attached in insulated base material layer,
(2) reagent solution not containing agglutinin after stirring is assigned on the graphite electrode of serigraphy making by a some liquid mode, and then dry 15 minutes at temperature 55 DEG C, reagent layer is fixedly worked on electrode,
(4) the reagent solution process porous nethike embrane (selecting the Polyester nethike embrane of Sefar) of the process nethike embrane containing agglutinin after stirring, then dries 15 minutes, adheres on reagent layer after drying by this nethike embrane layer at temperature 55 DEG C,
(4) space layer of reaction chamber of fitting on electrode system and reagent layer and overlayer, complete, wherein: with the adhesive tape with base material for space layer material, be bonded on electrode system and reagent layer after processing.
embodiment 3
The present embodiment structure and process are with embodiment 1, but reagent solution component is different, composed as follows:
Phosphate dihydrate disodium hydrogen: 1.1395g
Sodium dihydrogen phosphate dihydrate: 2.1223g
Sodium carboxymethyl cellulose (Sigma C5678): 1.0000g
Gelatin (Sigma G7041): 0.0500g
Triton X-100：0.2000g
Polyglycol (Sigma 95904): 0.5000g
The potassium ferricyanide: 3.2900g
Glucose oxidase (Amano, GO-AM): 600KU
Anti-human erythrocyte antibody: 0.01g
Purified water: 92.000g,
Reagent solution containing agglutinin passes through to stir, and makes it abundant dissolving; Then add purified water and be settled to 100mL, continue to be stirred to uniform composition, for subsequent use.
Then biology sensor is made into according to embodiment 1 preparation method.
embodiment 4
The present embodiment structure and process are with embodiment 2, and reaction reagent solution is also identical with embodiment 2, but different, composed as follows containing the reagent solution component of the process nethike embrane of agglutinin:
Sodium carboxymethyl cellulose (Sigma C5678): 1.0000g
Triton X-100：0.2000g
Anti-human erythrocyte antibody: 0.01g
Purified water: 98.000g,
Reagent solution containing the process nethike embrane of agglutinin passes through to stir, and makes it abundant dissolving; Then add purified water and be settled to 100mL, continue to be stirred to uniform composition, for subsequent use.
Then biology sensor is made into according to embodiment 2 preparation method.
embodiment 5
The present embodiment structure and process are with embodiment 1, but reagent solution agglutinin content is different, composed as follows:
Phosphate dihydrate disodium hydrogen: 1.1395g
Sodium dihydrogen phosphate dihydrate: 2.1223g
Sodium carboxymethyl cellulose (Sigma C5678): 1.0000g
Gelatin (Sigma G7041): 0.0500g
Triton X-100：0.2000g
Polyglycol (Sigma 95904): 0.5000g
The potassium ferricyanide: 3.2900g
Glucose oxidase (Amano, GO-AM): 600KU
Lectin (Kidney bean) (Sigma L8754): 0.0100g
Purified water: 92.7000g,
Reagent solution containing agglutinin passes through to stir, and makes it abundant dissolving, uniform composition, for subsequent use.
Then biology sensor is made into according to embodiment 1 preparation method.
embodiment 6
The present embodiment structure and process are with embodiment 1, but reagent solution agglutinin content is different, composed as follows:
Phosphate dihydrate disodium hydrogen: 1.1395g
Sodium dihydrogen phosphate dihydrate: 2.1223g
Sodium carboxymethyl cellulose (Sigma C5678): 1.0000g
Gelatin (Sigma G7041): 0.0500g
Triton X-100：0.2000g
Polyglycol (Sigma 95904): 0.5000g
The potassium ferricyanide: 3.2900g
Glucose oxidase (Amano, GO-AM): 600KU
Lectin (Kidney bean) (Sigma L8754): 1.0000g
Purified water: 92.7000g,
Reagent solution containing agglutinin passes through to stir, and makes it abundant dissolving, uniform composition, for subsequent use.
Then biology sensor is made into according to embodiment 1 preparation method.
embodiment 7
The present embodiment structure and process are with embodiment 1, but reagent solution agglutinin kind is different, composed as follows:
Phosphate dihydrate disodium hydrogen: 1.1395g
Sodium dihydrogen phosphate dihydrate: 2.1223g
Sodium carboxymethyl cellulose (Sigma C5678): 1.0000g
Gelatin (Sigma G7041): 0.0500g
Triton X-100：0.2000g
Polyglycol (Sigma 95904): 0.5000g
The potassium ferricyanide: 3.2900g
Glucose oxidase (Amano, GO-AM): 600KU
Lectin (Kidney bean) (Sigma L7647): 0.1000g
Purified water: 92.7000g,
Reagent solution containing agglutinin passes through to stir, and makes it abundant dissolving, uniform composition, for subsequent use.
Then biology sensor is made into according to embodiment 1 preparation method.
comparative example
A kind of method for making of traditional blood sugar biosensor:
Not containing the reagent solution of agglutinin, composed as follows:
Phosphate dihydrate disodium hydrogen: 1.1395g
Sodium dihydrogen phosphate dihydrate: 2.1223g
Sodium carboxymethyl cellulose (Sigma C5678): 1.0000g
Gelatin (Sigma G7041): 0.0500g
Triton X-100：0.2000g
Polyglycol (Sigma 95904): 0.5000g
The potassium ferricyanide: 3.2900g
Glucose oxidase (Amano, GO-AM): 600KU
Purified water: 92.7000g,
Reagent, by stirring, makes it abundant dissolving, uniform composition.Then be assigned on the graphite electrode of serigraphy making by some liquid mode, within 15 minutes, dry for 55 degrees Celsius, the space layer of laminating reaction chamber and overlayer, complete.
experimental example
Preparation has the blood sample of Different Red specific volume of cell 20%, 40%, 60%, and often kind of sample is mixed with 150mg/dL, 400mg/dL two kinds of blood sugar concentrations respectively.On electrochemical workstation, carry out chrono-amperometric test with these six kinds of blood and the biology sensor made, operating voltage is 0.36V, records 5 seconds current values after each test sample introduction.Calculate the deviation of the often kind of each blood sample of sensor 5 seconds electric current averages and 40% hematocrit value sample, 5 seconds electric current averages, 40% hematocrit value is the hematocrit value intermediate value of normal population, the deviation of height two ends hematocrit value sample test result and 40% hematocrit value sample test result describes the susceptibility of sensor to hematocrit value, difference is larger, susceptibility is higher, difference is less, and susceptibility is lower.The test result of embodiment and comparative example is shown in Fig. 3 ~ Figure 10 and following form:
the result of embodiment 1:
the result of embodiment 2:
the result of embodiment 3:
the result of embodiment 4:
the result of embodiment 5:
the result of embodiment 6:
the result of embodiment 7:
the result of comparative example:
Result shows, 7 kinds of comparative examples added in reagent without agglutinin in the height two ends hematocrit value sample test result of the embodiment of agglutinin and the deviation ratio reagent of 40% hematocrit value sample test result are less, illustrate that the reduction hematocrit value susceptibility of the present invention to sensor has actual effect.
Claims (10)
1. a biology sensor, it is characterized in that composition comprises insulated base material layer, be attached to the electrode system in insulated base material layer, described electrode system at least comprises a working electrode and one to electrode, cover the reagent layer above electrode system, described reagent layer at least should cover a working electrode, and the reaction chamber be positioned at above electrode system and reagent layer, described reaction chamber is made up of space layer and overlayer, and reaction chamber has bleeder vent, wherein: the component of described reagent layer contains red cell agglutination agent.
2. a kind of biology sensor according to claim 1, is characterized in that, described red cell agglutination agent is agglutinin or anti erythrocyte antibody, and agglutinin comprises animal origin agglutinin and phytolectin; Anti erythrocyte antibody comprises monoclonal antibody and polyclonal antibody.
3., according to the preparation method of a kind of biology sensor one of claim 1-2 Suo Shu, it is characterized in that comprising:
(1) in insulated base material layer, electrode system is formed, described electrode system at least comprises a working electrode and one to electrode, job operation has: by serigraphy, specific electrode pattern is printed onto in insulated base material layer, then by heating or infrared or ultraviolet is dry; Or electrode material is attached in insulated base material layer by the mode of sputtering, then processes specific electrode pattern by the mode of laser ablation,
(2) reagent layer is fixed on electrode by some liquid, serigraphy, inkjet printing or the processing of crack extrusion method,
(3) space layer of reaction chamber of fitting on electrode system and reagent layer and overlayer, complete, wherein: with base material or the adhesive tape not with base material for space layer material, be bonded on electrode system and reagent layer after processing; Or with glue or polymer paste for space layer material, by serigraphy on electrode system and reagent layer.
4. a biology sensor, it is characterized in that composition comprises insulated base material layer, be attached to the electrode system in insulated base material layer, described electrode system at least comprises a working electrode and one to electrode, cover the reagent layer above electrode system, described reagent layer at least should cover a working electrode, adhere to the nethike embrane layer on reagent layer, and the reaction chamber be positioned at above electrode system and nethike embrane layer, described reaction chamber is made up of space layer and overlayer, and reaction chamber has bleeder vent, wherein: described nethike embrane layer is the nethike embrane layer of red cell agglutination agent process.
5. a kind of biology sensor according to claim 4, is characterized in that, described red cell agglutination agent is agglutinin or anti erythrocyte antibody, and agglutinin comprises animal origin agglutinin and phytolectin; Anti erythrocyte antibody comprises monoclonal antibody and polyclonal antibody.
6. the preparation method of a kind of biology sensor as described in one of claim 4-5, is characterized in that, comprising:
(1) in insulated base material layer, electrode system is formed, described electrode system at least comprises a working electrode and one to electrode, job operation has: by serigraphy, specific electrode pattern is printed onto in insulated base material layer, then by heating or infrared or ultraviolet is dry; Or electrode material is attached in insulated base material layer by the mode of sputtering, then processes specific electrode pattern by the mode of laser ablation,
(2) reagent layer is fixed on electrode by some liquid, serigraphy, inkjet printing or the processing of crack extrusion method,
(3) with containing red cell agglutination agent solution process porous nethike embrane, after drying, this nethike embrane layer is adhered on reagent layer,
(4) space layer of reaction chamber of fitting on electrode system and nethike embrane layer and overlayer, complete, wherein: with base material or the adhesive tape not with base material for space layer material, be bonded on electrode system and nethike embrane layer after processing; Or with glue or polymer paste for space layer material, by serigraphy on electrode system and nethike embrane layer.
7. the application of biology sensor in blood testing, is characterized in that, described biology sensor composition comprises the immobilized biological sensitive materials of employing and makes recognition component, and biological sensitive materials is containing red cell agglutination agent.
8. the application of a kind of biology sensor according to claim 7 in blood testing, it is characterized in that described red cell agglutination agent is agglutinin or anti erythrocyte antibody, agglutinin comprises animal origin agglutinin and phytolectin; Anti erythrocyte antibody comprises monoclonal antibody and polyclonal antibody.
9. the application of red cell agglutination agent in a kind of reagent composition reducing hematocrit value susceptibility, is characterized in that, when described red cell agglutination agent is agglutinin, in reagent composition, working concentration interval is 0.01% ~ 5.0%; When described red cell agglutination agent is anti erythrocyte antibody, in reagent composition, working concentration interval is 1ug/mL ~ 10mg/mL.
10. application according to claim 9, is characterized in that, described red cell agglutination agent is agglutinin or anti erythrocyte antibody, and agglutinin comprises animal origin agglutinin and phytolectin; Anti erythrocyte antibody comprises monoclonal antibody and polyclonal antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510424013.6A CN104931560A (en) | 2015-07-17 | 2015-07-17 | Biosensor and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510424013.6A CN104931560A (en) | 2015-07-17 | 2015-07-17 | Biosensor and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104931560A true CN104931560A (en) | 2015-09-23 |
Family
ID=54118829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510424013.6A Pending CN104931560A (en) | 2015-07-17 | 2015-07-17 | Biosensor and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104931560A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770187A (en) * | 2016-11-21 | 2017-05-31 | 东南大学 | A kind of electrochromic devices array and its preparation, application method |
| CN108872322A (en) * | 2018-08-22 | 2018-11-23 | 江苏鱼跃医疗设备股份有限公司 | With can automatic identification distinguish information test paper |
| CN109900689A (en) * | 2019-03-28 | 2019-06-18 | 长沙中生众捷生物技术有限公司 | Interference rejection membrane and liver function joint test item |
| CN111474352A (en) * | 2020-04-29 | 2020-07-31 | 北京乐普医疗科技有限责任公司 | Novel coronavirus COVID-2019 detection card and preparation method thereof |
| CN111624246A (en) * | 2020-06-18 | 2020-09-04 | 桂林乐尔医疗器械有限公司 | Detection formula of uric acid biosensor |
| DE212020000554U1 (en) | 2020-06-30 | 2022-01-20 | Jiangsu Yuyue Information Technology System Co., Ltd. | Biosensor with reduced hematocrit interference |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62228273A (en) * | 1986-03-29 | 1987-10-07 | Fujitsu Ltd | Membrane of immobilized enzyme and enzymic electrode |
| US5296192A (en) * | 1992-04-03 | 1994-03-22 | Home Diagnostics, Inc. | Diagnostic test strip |
| EP1271147A1 (en) * | 2000-03-17 | 2003-01-02 | Sanwa Kagaku Kenkyusho Co., Ltd. | Test paper |
| US20060201805A1 (en) * | 2002-10-23 | 2006-09-14 | Forrow Nigel J | Biosensor having improved hematocrit and oxygen biases |
| US20080149480A1 (en) * | 2006-12-22 | 2008-06-26 | Home Diagnostics, Inc. | Gel formation to reduce hematocrit sensitivity in electrochemical test |
| CN101520430A (en) * | 2009-02-24 | 2009-09-02 | 上海大学 | Method for manufacturing biological detector of field effect transistor based on carbon nano tube |
-
2015
- 2015-07-17 CN CN201510424013.6A patent/CN104931560A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62228273A (en) * | 1986-03-29 | 1987-10-07 | Fujitsu Ltd | Membrane of immobilized enzyme and enzymic electrode |
| US5296192A (en) * | 1992-04-03 | 1994-03-22 | Home Diagnostics, Inc. | Diagnostic test strip |
| EP1271147A1 (en) * | 2000-03-17 | 2003-01-02 | Sanwa Kagaku Kenkyusho Co., Ltd. | Test paper |
| US20040022678A1 (en) * | 2000-03-17 | 2004-02-05 | Yoshiyuki Komagoe | Test paper |
| US20060201805A1 (en) * | 2002-10-23 | 2006-09-14 | Forrow Nigel J | Biosensor having improved hematocrit and oxygen biases |
| US20080149480A1 (en) * | 2006-12-22 | 2008-06-26 | Home Diagnostics, Inc. | Gel formation to reduce hematocrit sensitivity in electrochemical test |
| CN101520430A (en) * | 2009-02-24 | 2009-09-02 | 上海大学 | Method for manufacturing biological detector of field effect transistor based on carbon nano tube |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770187A (en) * | 2016-11-21 | 2017-05-31 | 东南大学 | A kind of electrochromic devices array and its preparation, application method |
| CN106770187B (en) * | 2016-11-21 | 2020-02-18 | 东南大学 | Electrochromic sensor array and preparation and use methods thereof |
| CN108872322A (en) * | 2018-08-22 | 2018-11-23 | 江苏鱼跃医疗设备股份有限公司 | With can automatic identification distinguish information test paper |
| CN109900689A (en) * | 2019-03-28 | 2019-06-18 | 长沙中生众捷生物技术有限公司 | Interference rejection membrane and liver function joint test item |
| CN109900689B (en) * | 2019-03-28 | 2021-12-10 | 复星诊断科技(长沙)有限公司 | Anti-interference membrane and liver function combined test strip |
| CN111474352A (en) * | 2020-04-29 | 2020-07-31 | 北京乐普医疗科技有限责任公司 | Novel coronavirus COVID-2019 detection card and preparation method thereof |
| CN111474352B (en) * | 2020-04-29 | 2023-12-22 | 北京乐普诊断科技股份有限公司 | Novel coronavirus COVID-2019 detection card and preparation method thereof |
| CN111624246A (en) * | 2020-06-18 | 2020-09-04 | 桂林乐尔医疗器械有限公司 | Detection formula of uric acid biosensor |
| DE212020000554U1 (en) | 2020-06-30 | 2022-01-20 | Jiangsu Yuyue Information Technology System Co., Ltd. | Biosensor with reduced hematocrit interference |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104931560A (en) | Biosensor and preparation method and application thereof | |
| Cao et al. | A novel 3D paper-based microfluidic electrochemical glucose biosensor based on rGO-TEPA/PB sensitive film | |
| Aro et al. | Saliva liquid biopsy for point-of-care applications | |
| Malon et al. | Saliva‐based biosensors: Noninvasive monitoring tool for clinical diagnostics | |
| JP5203620B2 (en) | Method and apparatus for analyzing a sample in the presence of interfering substances | |
| Singh et al. | Amperometric cholesterol biosensor based on immobilized cholesterol esterase and cholesterol oxidase on conducting polypyrrole films | |
| Parrilla et al. | based enzymatic electrode with enhanced potentiometric response for monitoring glucose in biological fluids | |
| US6033866A (en) | Highly sensitive amperometric bi-mediator-based glucose biosensor | |
| Moodley et al. | Historical perspectives in clinical pathology: a history of glucose measurement | |
| Pundir et al. | Biosensing methods for xanthine determination: A review | |
| CA2772738C (en) | Analyte measurement method and system | |
| AU2013224918B2 (en) | Test strip with stacked unidirectional contact | |
| JP2003501627A (en) | Disposable sensor and manufacturing method | |
| CN107064261A (en) | A kind of biology sensor and detection method based on glucose dehydrogenase | |
| JP2011080990A (en) | Multi-analyte test strip with inline working electrode and shared opposing counter/reference electrode | |
| CN106124595A (en) | A kind of biosensor and preparation method thereof and reaction reagent | |
| KR102051811B1 (en) | Biosensor for measuring glucose comprising cytoplasmic filter | |
| JP4516559B2 (en) | Biosensor using non-enzymatic uric acid reagent, biosensor test piece and method for producing the same | |
| Li et al. | Smart diaper based on integrated multiplex carbon nanotube-coated electrode array sensors for in situ urine monitoring | |
| CN107121478A (en) | A kind of biology sensor that can detect that sample is not enough, method | |
| CN104990913B (en) | A kind of method based on gold nano grain detection of the growth glucose | |
| US20030146111A1 (en) | Enzymatic-electrochemical measuring device | |
| WO2017161363A1 (en) | Point-of-care device for the colorimetric determination of hemoglobin and glucose-6-phsphate dehydrogenase in biological samples | |
| WO2019176339A1 (en) | Protective film material for biosensor probe | |
| JP3477511B2 (en) | Biosensor using gold platinum electrode |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150923 |
|
| RJ01 | Rejection of invention patent application after publication |