CN104911273B - A kind of chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken and its application - Google Patents
A kind of chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken and its application Download PDFInfo
- Publication number
- CN104911273B CN104911273B CN201510378546.5A CN201510378546A CN104911273B CN 104911273 B CN104911273 B CN 104911273B CN 201510378546 A CN201510378546 A CN 201510378546A CN 104911273 B CN104911273 B CN 104911273B
- Authority
- CN
- China
- Prior art keywords
- chicken
- fabp1
- site
- genotype
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken, it is the pleomorphism site in chicken FABP1 gene mononucleotide polymorphism sequences, the site is in the 83rd nucleotide polymorphisms site for C or T of FABP1 gene order Second Exons, tri- kinds of genotype of CC, CT or TT are shown as, wherein TT types are the excellent slaughter trait molecular genetic marker mark of meat chicken.Assisting sifting is marked at the invention also discloses described or prediction possesses application in the carnivorous chicken breed of excellent slaughter trait.Experiment is confirmed：Possesses correlation between the polymorphism of Idiotype restriction enzyme site and production performance, it is CC and CT individual that the most measurement indexes of individual that genotype is TT, which are above genotype, points out genotype to cultivate the kind chicken with excellent slaughter trait as breeder for TT individual.
Description
Technical field
The invention belongs to field of molecular biotechnology, be related to a kind of meat chicken excellent slaughter trait molecular genetic marker and
Its assisting sifting or prediction possess the carnivorous chicken breed of excellent slaughter trait in application.
Background technology
At present for molecular genetic marker more complete description, refer to it is readily identified, follow Mendelian inheritance pattern,
There is a certain class table feature or inhereditary material of germplasm feature with individual specificity or the regularity of distribution, its scope includes：Base
Because or inhereditary material product variation features, as the morphological variant of gene or the chromosome of inhereditary material carrier, gene or
The variation of inhereditary material in itself etc..DNA molecular Genetic Markers are a kind of emerging molecular marking techniques, are being divided at present
Sub- biology is particularly widely used in molecular genetics.Because Eukaryotic hereditary information is stored within dye
In colour solid and the DNA sequence dna of organelle gene group, therefore theoretically, the molecular labeling on DNA level is all heredity marks
Stablize the most in note the most reliable.The development of modern molecular biology technique directly to utilize DNA sequence dna nucleotide
Variation becomes possibility as genetic marker.
SNP (single nucleotide polymorphism, SNP) refers in DNA sequence
DNA sequence polymorphism caused by the variation of single nucleotide acid in level, and a kind of wherein minimum frequency of allele in colony
Rate is not less than 1%.It includes the forms such as conversion, transversion, insertion and the missing of base.Because SNP has high density, rich in generation
The advantages of automation of table, genetic stability and easy realization analysis, therefore as a class genetic marker, SNP is in hereditary credit
It is widely used in analysis.
Fatty acid binding protein (Fatty acid binding protein, FABP) is widely present in vertebrate and expense
In the cytoplasm of vertebrate, belong to lipid binding protein superfamily member.FABP albumen is albumen in family's small molecular cell,
Have very high affinity to long chain fatty acids, aliphatic acid can be transferred to from cell membrane it is intracellular, in the metabolism of long chain fatty acids
In play an important role.The FABP albumen of 9 types is had discovered that so far, and each type of FABP albumen is in different groups
Knit and expressed with cell.
FABP1 is liver type fatty acid binding protein, and a member belonged in FABP superfamilies, is present in liver cell and small intestine is viscous
Theca cell kytoplasm, has high affinity to palmitate, oleic acid and stearate etc., participates in the intake of aliphatic acid and turns
Fortune.In liver, long chain fatty acids are typically absorbed into born of the same parents by the FABP fatty acid transport proteins on liver plasma membrane, are then existed
Mitochondria, peroxisome, endoplasmic reticulum etc. are transported under FABP1 auxiliary and carries out beta oxidation, so that in regulating cell
Fatty acid metabolism, finally makes body fat acid metabolic keep relative equilibrium.As molecular labeling, FABP1 shows high heredity
Polymorphism.Fatty character is the important indicator for evaluating chicken meat quality, and the development of such character can directly affect its commodity performance.Cause
This, for regulate and control fatty character development gene SNPs sign for improving it by the molecular breeding and assisted Selection of broiler chicken
Production performance has a very important role.It is related in applicant's related experiment in the colony of chicken, FABP1 mononucleotides are unmutated
Its individual meat productivity and meat be superior to homozygous mutation individual and heterozygous mutant individual, intuitively embodied from data
Influences of the FABP1 gene SNPs s to the chicken production traits.If this discovery is applied in the research work such as the breeding of high-quality poultry, have
Hope and provide help to cultivate the improved seeds of high yield and high quality meat character.Through retrieval, current FABP1 gene SNPs s is marked as heredity
Note, the excellent slaughter trait molecular genetic marker especially as meat chicken is applied to screening or prediction possesses excellent slaughter trait
Carnivorous chicken breed have not been reported.
The content of the invention
In view of the shortcomings of the prior art, the problem to be solved in the present invention is to provide a kind of related to the excellent production traits of chicken
Chicken FABP1 gene molecules genetic marker and its answering in assisting sifting or prediction possess the carnivorous chicken breed of excellent slaughter trait
With.
The chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken of the present invention is chicken FABP1 bases
Because of the pleomorphism site in mononucleotide polymorphism sequence, it is characterised in that：The chicken FABP1 gene mononucleotide polymorphism sequences
The pleomorphism site of row is the performance in the 83rd nucleotide polymorphisms site for C or T of FABP1 gene order Second Exons
For tri- kinds of genotype of CC, CT or TT, wherein TT types are the excellent slaughter trait molecular genetic marker of meat chicken.
The chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken of the present invention is in assisting sifting or pre-
Measuring tool is for the application in the carnivorous chicken breed of excellent slaughter trait.
The specific method of above-mentioned application is：Using chicken FABPl genomic DNAs to be detected as template, with primer pair FABP1
E2F/E2R enters performing PCR amplification, row agarose gel electrophoresis is entered after the completion of amplification, then using specific restriction nuclease inscribe
Enzyme carries out digestion to the purpose fragment amplified；Row agarose gel electrophoresis are entered again to digestion products afterwards, to detect enzyme
The clip size of product is cut, analysis determines the nucleotide polymorphisms site of chicken FABP1 genes, when FABP1 gene orders second are outer aobvious
Son the 83rd be C or T, and genotype be TT types when, you can judge chicken breed to be detected as the product with excellent slaughter trait
Breeder；
Wherein：
The nucleotides sequence of the primer pair FABP1 E2F/E2R is classified as：
FABP1 E2F：CTTGAGAGGCTCTTCCACAATAG；
FABP1 E2R：GTTGGACTGGATAGCCTTTAAGTTC；
The response procedures of PCR amplification are：95 DEG C of pre-degeneration 4-5min；94 DEG C of denaturation 30-35s, 55-61 DEG C of annealing
30-35s, 72 DEG C of extension 1min, 28-35 circulation, last 72 DEG C of extensions 10min is placed in 4 DEG C of preservations；
The specific restriction endonuclease is：ASP MDI, Bsp AI, MboI, CacI and HacI, its digestion are made
It is to amplify the following sequence in target gene with site：5’…↓GATC ... 3 ' or 3 ' ... CTAG↑…5’。
The above method is preferred embodiment：For FABP1 E2F/E2R primer pairs, PCR amplification programs are：95
DEG C pre-degeneration 4min；94 DEG C of denaturation 30s, 57 DEG C of annealing 30s, 72 DEG C of extension 1min, 35 circulate, last 72 DEG C of extensions 10min,
It is placed in 4 DEG C of preservations.The specific restriction endonuclease is：MboI.
The present invention is using on digestion method rapid screening chicken FABP1 gene Second Exons site in restriction nuclease
The SNP of generation is detected, carries out Genotyping by the SNP to chicken breed and gene frequency is analyzed, simultaneously
To being associated analysis between digestion polymorphic site and chicken growth traits, find the SNPs related to chicken growth traits and be used as molecule
Mark, and the excellent slaughter trait molecular genetic marker using function SNPs as meat chicken, possess in assisting sifting or prediction
Extensive use in the carnivorous chicken breed of excellent slaughter trait, accelerates the seed selection speed of breeding chicken and improves population quality.
The present invention is by using SNP site as restriction enzyme site, selecting suitable specific endonucleases, then according to SNP
Digestion goes out after mutation DNA fragmentation is of different sizes to determine chicken FABP1 gene mononucleotide polymorphism features.
According to chicken FABP1 gene mononucleotide polymorphism features, present invention determine that chicken FABP1 gene mononucleotide polymorphisms
The pleomorphism site of sequence is in the 83rd nucleotide polymorphisms site for C or T of FABP1 gene order Second Exons, table
It is now tri- kinds of genotype of CC, CT or TT, wherein TT types are the excellent slaughter trait molecular genetic marker target of meat chicken.Experiment hair
It is existing：The chicken of tri- kinds of genotype of CC, CT or TT has very big difference, butchering property of TT type chickens in terms of meat productivity and fleshy optimization
Shape (meat productivity and meat) is substantially better than CC, CT two types, so the chick of the genotype can be as possessing excellent Tu
Kill the breeder of character to cultivate, this has also affirmed that the chicken that genotype is TT can be cultivated with excellent slaughter trait as breeder
Kind chicken exploitativeness.Application process of the present invention is simple, quick, inexpensive, easy to utilize, can be widely applied to chicken
Assisted Selection and molecular breeding in, support will be provided for the improved seeds for cultivating high yield and high quality meat character.
Brief description of the drawings
Fig. 1 is the practical application schematic flow sheet of the excellent slaughter trait molecular genetic marker of meat chicken of the present invention.
The FABP1 encoding genes structural representation for the chicken that Fig. 2 expands for the present invention and Second Exon sequence.
Wherein, 83 nucleotides underscores of Second Exon and black matrix amplification letter signal.In wild gene, the
It in T, mutator is C that 83 nucleotides, which are,.
Fig. 3 is the signal of gained clip size after different genotype FABP1 genes restriction enzyme site of the present invention and digestion
Figure.
The chicken FABP1 gene order agaroses that Fig. 4 arrives for part sample P CR products in the present invention through specific digestion examination
Gel electrophoresis results figure.Wherein, TT is unmutated type, and CT is heterozygous mutant, and CC is homozygous mutant.
Embodiment
The present invention designs primer according to the conserved sequence of chicken FABP1 genes first, using different cultivars chicken genomic DNA as mould
Plate, enters performing PCR amplification, sequencing.Then, specific digestion is carried out, and by the agarose gel electrophoresis figure and sequence of digestion result
Compare examination and go out different cultivars chicken FABP1 gene SNP sites.Then, according to the genotype detected in colony, colony is carried out
Genetic statistics analysis and the association analysis of growth traits, filter out the molecular labeling closely related with chicken growth traits.Finally, it is sharp
Applied, will be screened in the carnivorous chicken breed that assisting sifting or prediction possess excellent slaughter trait with the molecular genetic marker of acquisition
To possess and cultivated with the chicken of the excellent slaughter trait related molecular marker of chicken as breeder, to breed more high-quality
Chicken.The present invention is elaborated below, the content is explanation of the invention rather than restriction.
Embodiment 1：The clone of different cultivars chicken FABP1 Gene Partial DNA sequence dnas
1st, the collection and processing of chicken blood：Randomly select disease-free healthy chicken, including 670 Type 3 Luqin chickens and 60 Lang Ya
Chicken, the venous blood collection under wing, EDTA does anti-freezing processing.
2nd, the extraction of genomic DNA：DNA is extracted using Tiangen blood DNAs extracts kit.Using 200 μ L it is fresh,
The blood of various anti-coagulants is freezed or added, 20 μ L Proteinase K Solutions are added, is mixed；Then 200 μ L buffer solution GB are added, are mixed
Placed 10 minutes for 70 DEG C after even；200 μ L absolute ethyl alcohols are added, fully vibration is mixed, and flocculent deposit now occurs；Gained is molten
Liquid and flocculent deposit are transferred completely into the adsorption column CB3 being put into collecting pipe, and 12000rpm is centrifuged 30 seconds and abandoned waste liquid；Add
500 μ L buffer solutions GD, 12000rpm centrifugations 30 seconds simultaneously abandon waste liquid；700 μ L rinsing liquids PW (adding absolute ethyl alcohol using preceding) are added,
12000rpm is centrifuged 30 seconds and is abandoned waste liquid, and repeats the step twice；Thoroughly eliminate after the rinsing liquid in sorbing material, use
Water or eluent TE elute gained DNA product as far as possible.
3rd, amplimer is designed：According to (http in ncbi database://www.ncbi.nlm.nih.gov/) chicken
GenBank accession number is：The exon sequence of reflNP_989523.1 FABP1 genes, with the gene order conserved region sequence
For Reference Design PCR primer pair, its primer pair sequence is as follows：
Sense primer (FABP1 E2F)：CTTGAGAGGCTCTTCCACAATAG；
Anti-sense primer (FABP1 E2R)：GTTGGACTGGATAGCCTTTAAGTTC；
The primer has expanded FABP1 gene Second Exon 447bp sequences.
4th, PCR expands chicken FABP1 gene extrons：Using the DNA of chicken as masterplate, performing PCR is entered with the primer pair of above-mentioned design
Amplification, PCR overall reactions system is 30 μ L, is shown in Table 1；PCR overall reaction programs, are shown in Table 2.
The PCR reaction systems of table 1
The PCR response procedures optimal in the scope of application of table 2
Embodiment 2：Specific digestion is carried out to PCR primer in embodiment 1 using MboI enzymes
1. specific digestion is carried out to target gene using MboI enzymes.The action site of this kind of enzyme-specific is：
5’…↓GATC…3’
3’…CTAG↑…5’
2. endonuclease reaction system is shown in Table 3.
The MboI endonuclease reaction systems of table 3
After each solution of reaction system is mixed according to above-mentioned system, it is positioned in 37 DEG C of waters bath with thermostatic control and reacts 3-5h, make enzyme
Cut reaction fully to carry out, follow-up test is carried out afterwards.
3. a pair digestion products enter row agarose gel electrophoresis analysis.Then according to different digestion results by all samples
Classification summary is carried out, different types are summarized.
Digestion result has three kinds, is SNP site full mutation, heterozygous mutant respectively and is not mutated completely.SNP
In the case of point full mutation, MboI has point of contact at three in purpose fragment, obtain four different size of bands (5bp,
63bp, 146bp and 233bp)；In the case of the non-full mutation of SNP site, MboI has three restriction enzyme sites in purpose fragment,
Obtain five bands (5bp, 63bp, 146bp, 233bp and 296bp) of different sizes；In the case that SNP site is unmutated,
MboI has two restriction enzyme sites in purpose fragment, and digestion products size is (5bp, 146bp and 296bp).Because of 5bp and 64bp pieces
Duan Feichang is small, and the bar segment of 146bp, 233bp and 296bp tri- (as shown in Figure 3) is only detected in the sugared electrophoresis of plain agar.
The nucleotide site marked in sequence below with square frame is polymorphic site, and the site has two kinds of genotype of C/T,
When genotype is T, PCR primer can only be cut into three bar segments by restriction endonuclease, but electrophoresis can detect two of which (146bp
And 296bp)；When genotype is C, PCR primer can be cut into more shorter fragments by restriction endonuclease.
TTCAAGATACTGTGACTA
Embodiment 3：The diagnostic application of molecular genetic marker prepared by the present invention in different chicken colonies polymorphism
1st, the diagnosis in colony's polymorphism：Using above-mentioned SNP pleiomorphism detecting methods to 670 parts of Type 3 Luqin chickens and 60
Part thinkling sound Ya chicken FABP1 genes carry out specific digestion detection.
2nd, the frequency statistics analysis of SNP site：
Genotype frequency refers to that the number of certain specific gene type individual in a colony accounts for the ratio of individual total number.Paa
=Aaa/N, wherein Paa represent the AA genotype frequencies in a certain site, and Aaa represents the number of individuals in colony with AA genotype, N
To detect the total quantity of colony.
Gene frequency refers to relative ratios of a certain gene number to its allele sum in a colony.The formula of calculating
It can be write as：PA=(2NM+NAal+NAa2+......+NAan)/2N.In formula, PA represents allele A frequencies, and NM represents colony
In have AA genotype individual amount, Aal-anFor allele A n different multiple alleles.Statistical result is shown in Table 6 Hes
Table 7.
The genotype and gene frequency in the Type 3 Luqin chicken FABP1 gene polymorphics site of table 6
The genotype and gene frequency in the thinkling sound's Ya chicken FABP1 gene polymorphics site of table 7
3rd, the association analysis of gene effect
Using PASW (18.0) statistical software, to Type 3 Luqin chicken and Shiqiza different genotype individual and body footage evidence
Significance test is carried out.
(1) major traits determined include：Slaughter weight, slaughter traits, dressing percentage, half net thorax weight, complete net thorax weight, half net thorax
Rate, complete net thorax rate, chest muscle weight, chest muscle rate, leg flesh weight, leg flesh rate, abdominal fat weight, abdominal fat, sebum thickness, flesh fat are wide etc..
(2) colony determined：Randomly select healthy disease-free Type 3 Luqin chicken 670 and only reach thinkling sound Ya chickens 60 and only analyzed respectively.
Statistical result is shown in Table 8 and table 9.
The association analysis of the Type 3 Luqin chicken FABP1 E2 polymorphisms of table 8 and slaughter trait
The association analysis of the thinkling sound's Ya chickens FABP1 E2 polymorphisms of table 9 and slaughter trait
| FABP1SNP sites | C/C(3)Mean±SD | C/T(12)Mean±SD | T/T(45)Mean±SD |
| Slaughter weight | 1332.67±300.55 | 1389.03±76.32 | 1335.87±39.12 |
| Slaughter traits | 1194.57±274.12 | 1260.40±66.14 | 1196.66±34.84 |
| Carcass rate | 89.39±0.10 | 90.90±0.68 | 89.67±0.52 |
| Half net thorax weight | 1100.23±254.94 | 1140.49±61.12 | 1100.22±31.95 |
| Half net thorax rate | 82.29±0.62 | 82.26±0.80 | 82.49±3.72 |
| Complete net thorax weight | 1002.33±233.23 | 1039.56±57.43 | 996.60±30.35 |
| Complete net thorax rate | 74.95±1.03 | 74.89±3.02 | 74.61±3.59 |
| Chest muscle weight | 144.75±26.74 | 139.84±6.36 | 158.83±114.48 |
| Chest muscle rate | 15.14±0.88 | 13.56±0.26 | 16.53±2.32 |
| Leg flesh weight | 177.53±47.88 | 190.42±11.43 | 182.25±6.14 |
| Leg flesh rate | 17.42±0.66 | 18.29±17.63 | 18.28±0.23 |
| Abdominal fat weight | 17.96±9.92 | 25.36±5.17 | 17.91±1.70 |
| Abdominal fat | 1.51±0.53 | 2.13±0.42 | 1.86±0.19 |
| Sebum is thick | 3.24±0.66 | 4.02±0.37 | 4.68±0.25 |
| Flesh fat is wide | 5.43±0.55 | 6.02±0.45 | 5.67±0.28 |
| Wing weight | 103.59±36.72 | 73.00±10.79 | 87.10±5.50 |
| Wing rate | 11.47±0.35 | 10.90±0.22 | 11.17±0.20 |
| Leg weight | 279.51±103.60 | 264.75±76.22 | 236.30±15.51 |
| Leg ratio | 30.53±0.89 | 46.54±19.26 | 29.91±0.30 |
Note：Letter in genotype subscript shows the difference between different genotype, and two kinds of bases are represented with same letter
Because of the not notable (P of difference between type>0.05), letter is different represents significant difference (P between two kinds of genotype<0.05), do not mark
Note show be not significantly different between the genotype and other genotype.
Analysis display, in Type 3 Luqin chicken genotype be TT its individual Slaughter weight, slaughter traits, half net thorax weight, Quan Jing
Thorax weight, chest muscle weight and leg flesh again etc. measurement index with CC types and CT types individual difference significantly, and TT types measured value maximum.Together
Sample, in thinkling sound Ya chickens, for CC individuals, the measurement data of the TT types individual production traits is even more ideal, i.e. TT types chicken
It is more suitable as screening and breeding that breeder carries out excellent kind chicken.
Claims (1)
1. a kind of chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken possesses excellent in assisting sifting or prediction
Application in the Type 3 Luqin kind chicken of good slaughter trait；
It is characterized in that：
The mark is pleomorphism site in chicken FABP1 gene mononucleotide polymorphism sequences, the pleomorphism site be
The 83rd of FABP1 gene order Second Exons is C or T nucleotide polymorphisms site, shows as tri- kinds of genes of CC, CT or TT
Type, wherein TT types are the excellent slaughter trait molecular genetic marker of meat chicken；When agarose gel electrophoresis determines chicken to be detected
The 83rd of FABP1 gene order Second Exons be C or T, and genotype be TT types when, you can judge chicken breed to be detected as
Type 3 Luqin kind chicken with excellent slaughter trait；
Wherein, the experimental method of the agarose gel electrophoresis is：Using Type 3 Luqin kind chicken FABPl genomic DNAs to be detected as
Template, enters performing PCR amplification with primer pair FABP1 E2F/E2R, row agarose gel electrophoresis is entered after the completion of amplification, then using spy
Fixed restriction endonuclease MboI carries out digestion to the purpose fragment amplified, and its digestion action site is to amplify purpose
Following sequence in gene：5’…↓GATC ... 3 ' or 3 ' ... CTAG↑…5’；Afterwards digestion products are carried out with agarose again to coagulate
Gel electrophoresis, to detect the clip size of digestion products, analysis determines the nucleotide polymorphisms site of chicken FABP1 genes；
Above-mentioned primer pair FABP1 E2F/E2R nucleotides sequence is classified as：
FABP1 E2F：CTTGAGAGGCTCTTCCACAATAG；
FABP1 E2R：GTTGGACTGGATAGCCTTTAAGTTC；
The response procedures of PCR amplification are：95 DEG C of pre-degeneration 4min；94 DEG C of denaturation 30s, 57 DEG C of annealing 30s, 72 DEG C of extensions
1min, 35 circulations, last 72 DEG C of extensions 10min is placed in 4 DEG C of preservations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510378546.5A CN104911273B (en) | 2015-07-01 | 2015-07-01 | A kind of chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510378546.5A CN104911273B (en) | 2015-07-01 | 2015-07-01 | A kind of chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104911273A CN104911273A (en) | 2015-09-16 |
| CN104911273B true CN104911273B (en) | 2017-10-31 |
Family
ID=54080713
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510378546.5A Expired - Fee Related CN104911273B (en) | 2015-07-01 | 2015-07-01 | A kind of chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104911273B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755447B (en) * | 2017-01-04 | 2020-06-09 | 安徽农业大学 | Molecular marker-assisted selection method for reducing abdomen fat rate of white-feather king pigeon and young pigeon |
| CN108251539B (en) * | 2018-02-06 | 2021-09-07 | 河南农业大学 | SNP (Single nucleotide polymorphism) marker related to chicken carcass traits and application thereof, detection primer and detection kit |
| CN110872612B (en) * | 2019-12-19 | 2021-02-09 | 南昌师范学院 | Detection method for correlation between VIPR2 gene 3' regulatory locus point and chicken testicular character and application |
| CN114150071B (en) * | 2020-09-08 | 2023-07-25 | 河南农业大学 | Application of chicken TRIM13 gene SNP molecular marker in chicken growth and slaughter trait improvement breeding and breeding method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1982478A (en) * | 2004-08-06 | 2007-06-20 | 张沅 | Method for inspecting chicken fat quale by mononucleotide polymorphism |
| WO2011006070A2 (en) * | 2009-07-09 | 2011-01-13 | Interleukin Genetics, Inc. | Genetic association of polymorphisms in perilipin (plin) gene with resistance to weight loss |
-
2015
- 2015-07-01 CN CN201510378546.5A patent/CN104911273B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1982478A (en) * | 2004-08-06 | 2007-06-20 | 张沅 | Method for inspecting chicken fat quale by mononucleotide polymorphism |
| WO2011006070A2 (en) * | 2009-07-09 | 2011-01-13 | Interleukin Genetics, Inc. | Genetic association of polymorphisms in perilipin (plin) gene with resistance to weight loss |
Non-Patent Citations (7)
| Title |
|---|
| Identification of SNPs of the L-BABP and L-FABP and their Association with Growth and Body Composition Traits in Chicken;Yufang Zhao等;《Japan Poultry Science》;20130425;第50卷;第303页表2和第308页表6 * |
| Lb-FABP和L-FABP与鸡生长和体组成性状的关联及其配基结合属性研究;赵玉芳;《中国优秀硕士学位论文全文数据库》;20131015;第21-22,27-28页 * |
| Submitted SNP(ss)Details:ss947848613;Submitter;《dbSNP》;20140127;第1页 * |
| Tissue Expression and Association with Fatness Traits of Liver Fatty Acid-Binding Protein Gene in Chicken;Q. Wang等;《Poultry Science》;20061231;第85卷;图5与表1 * |
| 脂肪酸结合蛋白及其主要家族基因的研究进展;徐海霞等;《畜牧与兽医》;20081231;第40卷(第1期);第94-97页 * |
| 鲁禽3号麻鸡优良肉质性状分子遗传标记筛选;王丹丹等;《中国家禽》;20160531;第38卷(第9期);第10-14页 * |
| 鸡FABP基因克隆、表达特性及功能研究;王启贵;《中国博士学位论文全文数据库》;20040315;第75-79页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104911273A (en) | 2015-09-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111705140B (en) | SNPs molecular marker related to weight traits and application thereof in Hu sheep assisted breeding | |
| CN110734983B (en) | SNP marker related to intramuscular fat character of Suhuai pig, detection method and application | |
| CN104911273B (en) | A kind of chicken FABP1 gene molecule genetic marker related to the excellent production traits of chicken and its application | |
| CN109371143B (en) | SNP molecular marker associated with pig growth traits | |
| CN108410994A (en) | It is a kind of influence sheep Fecundity Trait SNP marker and its application | |
| KR101770075B1 (en) | Pig production and traceability systems selected as a single nucleotide polymorphism markers for the introduction of the method | |
| CN104946776A (en) | Chicken FABP4 gene molecular genetic marker related to good chicken slaughtering character and application of chicken FABP4 gene molecular genetic marker | |
| CN109371144B (en) | SNP molecular marker associated with pig growth traits | |
| CN108998541B (en) | SNP (Single nucleotide polymorphism) marker primer pair related to hip circumference traits of Suhuai pig legs and application thereof | |
| CN108103208A (en) | A kind of SNP marker for influencing sheep Fecundity Trait and its application | |
| CN107937556A (en) | One and the relevant SNP site of pannage conversion ratio and its application | |
| CN108676897A (en) | It is a kind of influence daily gain in pigs character SNP marker and its application | |
| CN111926085B (en) | Molecular marker influencing chicken muscle brightness and application thereof | |
| CN111926086B (en) | Molecular marker influencing oblique growth of chicken body and application thereof | |
| CN108913779B (en) | SNP marker influencing daily gain traits of pigs and application thereof | |
| CN104673902A (en) | SNP molecular marker related to breast muscle weight and breast muscle percentage of chicken and application of SNP molecular marker | |
| CN106636429A (en) | Tetra-primer amplification refractory mutation system-PCR (polymerase chain reaction) method for detecting cattle ADNCR gene single nucleotide polymorphism and application of tetra-primer amplification refractory mutation system-PCR method | |
| CN104593363B (en) | Excellent slaughter trait molecular genetic marker of broiler chicken and application of excellent slaughter trait molecular genetic marker | |
| CN112176073B (en) | PROS1 gene molecular marker related to chicken carcass traits and application | |
| CN112575096A (en) | SNP molecular marker related to total papilla number of large white pigs and acquisition method thereof | |
| CN111088327B (en) | Method for detecting cattle body size characters under assistance of SIKE1 gene CNV marker and application thereof | |
| CN111269989B (en) | Pig MID1 gene as mortality-related molecular marker and application thereof | |
| CN111961732A (en) | Molecular marker influencing full bore weight of chicken and application thereof | |
| CN112779339A (en) | SNP molecular marker related to total papilla number of large white pigs as well as acquisition and application thereof | |
| CN106319063B (en) | A method of ox meat quality is detected using PRSS2 genetic marker |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171031 Termination date: 20190701 |