CN104904744A - Pesticide composition containing Dufulin and imidazole fungicide as well as fungicide - Google Patents

Abstract

The invention provides a pesticide composition and a fungicide. The pesticide composition contains Dufulin and imidazole fungicide, wherein the imiddazole fungicide is one or more of cyazofamid, prochloraz, triflumizole, pefurazoate, imazalil and fenamidone, and the weight ratio of Dufulin to the imidazole fungicide is (60-1):(1-30). The pesticide composition provided by the invention accounts for 0.1-90% of the total weight of the fungicide. Compared with single dosages, the pesticide composition and the fungicide have the advantages of improving control effect, reducing dosage and delaying drug resistance; and the pesticide composition and the fungicide can be used for treating viral diseases and also can be used for preventing and treating fungal diseases, so that the pesticide composition and the fungicide are ecological pesticides with high safety and extremely low toxicity.

Description

The composition pesticide of a kind of toxic fluorine phosphorus and imidazoles bactericide and bactericide

Technical field

The present invention relates to technical field of pesticide, particularly a kind of malicious fluorine phosphorus and the bactericide compounded composition pesticide of imidazoles and bactericide thereof.

Background technology

On nearly 2 years pesticide markets, domestic bactericide is sold and is glided always, and the preventive effect mainly controlling disease because of it can not show a candle to the preventive effect of external bactericide.And develop a kind of novel pesticide composition in China, Meteorological is high, and technology is immature.Though external novel pesticide composition drug effect is good, price is high.Therefore, utilizing the pesticide active ingredient of the different mechanism of action to carry out composite is the one mode effectively efficiently developed pesticide new product at present and prevent and treat resistance damage by disease and insect.Different pesticide active ingredient carry out composite after, three kinds of type of action can be shown: summation action, synergistic effect and antagonism.Usually, only have by a large amount of tests just can learn different pesticide active ingredient composite after type of action.

Poison fluorine phosphorus is that inventor is transferred the possession of the plant virus resistance product of new generation of coming by Guizhou University, and the patent No. is ZL200510003041.7, and it is a kind of novel Antiphytoviral medicament of fluorine-containing amino phosphonate ester of novel structure.Because of its characteristic such as novel structure, mechanism of action uniqueness, remarkably productive for preventing and treating virus disease.But in pesticide field, during good Pesticide use, also to note protection.Therefore, find different classes of pesticide species and its mixture uses, not only improve the control efficiency of malicious fluorine phosphorus, also delay the service life of excellent agricultural chemicals.

And imidazoles bactericide sterilization mechanism complexity is various, fungicidal spectrum is wide, has good protectiveness and permeability.But imidazoles bactericide because of service time long, pathogen is strengthened its resistance, preventive effect decline.Conventional imidazoles bactericide is mainly cyazofamid, Prochloraz, fluorine bacterium azoles, pefurazoate, imazalil, Fenamidone.Above-mentioned imidazoles bactericide is all known in the art and disclosed pesticide species.

Cyazofamid, No. CAS is 120116-88-3, is a kind of mitochondrial respiratory inhibitor, has very high biologically active to Oomycete fungal.

Prochloraz, No. CAS is 67747-09-5, is a kind of efficient, wide spectrum, low-toxicity bactericide, has the multiple actions such as prevention & protection treatment.

Fluorine bacterium azoles, CAS 68694-11-1 is broad-spectrum germicide, and sterol demethylation inhibitors has interior suction, protection, treatment, eradicant action.

Pefurazoate, No. CAS is 101903-30-4, is a kind of ergosterol demethylation inhibitors, and to kind of the disease fungus passed, the bakanae disease of rice particularly caused by Fusorium moniliforme Sheldon, rice blast have excellent control efficiency.The fungal disease that Ascomycetes, Basidiomycetes and deuteromycetes cause can be prevented and treated.

Imazalil, No. CAS is 35554-44-0, is the good bactericide of a kind of interior absorption.Can destroy the membrane structure of mould, sterilization is extensively general, can be used for control oranges and tangerines, mango, banana, apple, the crop pest such as melon, also can be used for control cereal disease.

Fenamidone, No. CAS is 161326-34-7, is a kind of mitochondrial respiratory inhibitor, and fungicidal spectrum is wide, and infecting caused most of fungal diseases to lower fungus has good protection therapeutic action.

A large amount of laboratory test and field control effectiveness test result show, malicious fluorine phosphorus and imidazoles bactericide compounded, there is obvious synergistic effect.Through retrieval, do not find the relevant report of the composition of malicious fluorine phosphorus and imidazoles bactericide.

Summary of the invention

For the deficiency that prior art exists, the invention provides a kind of composition pesticide and bactericide, this composition pesticide is containing poisonous fluorine phosphorus and imidazoles bactericide, described composition pesticide and bactericide have obvious synergistic function relative to single dose, both reduced pesticide usage amount, the pesticide resistance that can also delay pathogen produces.

For realizing above-mentioned object, the technical solution used in the present invention is:

There is provided a kind of composition pesticide, described composition pesticide is containing poisonous fluorine phosphorus and imidazoles bactericide, and wherein imidazoles bactericide is selected from one or more in cyazofamid, Prochloraz, fluorine bacterium azoles, pefurazoate, imazalil or Fenamidone; The weight ratio of described malicious fluorine phosphorus and imidazoles bactericide be (60 ~ 1) (: 1 ~ 30).

Preferably, the weight ratio of described malicious fluorine phosphorus and imidazoles bactericide is (20 ~ 1): (1 ~ 10).

The present invention also provides a kind of bactericide containing composition pesticide described above, and this composition pesticide accounts for the 0.1-90% of described bactericide gross weight.Preferably, composition pesticide described above accounts for the 6-70% of bactericide gross weight of the present invention.

Bactericide provided by the invention, except toxic fluorine phosphorus and imidazoles bactericide, also contains acceptable auxiliary element on agricultural chemicals.On its Pesticides, acceptable auxiliary element is the mixture of a kind of and auxiliary agent in solvent and filler.Also can add other conventional functionality auxiliary agents such as antifreezing agent, thickener, stabilizing agent, defoamer, disintegrant, film forming agent if desired.

Filler comprises kaolin, bentonite, diatomite, earth powder, ammonium dihydrogen phosphate (ADP), urea, montmorillonite, potassium sulfate.

Solvent is water.

Dispersant, wetting agent, emulsifier comprises sodium carboxymethylcellulose, sodium metnylene bis-naphthalene sulfonate, alkylphenol-polyethenoxy base phosphate, polycarboxylate, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, aliphatic alcohol polyethenoxy base ether, alkylbenzenesulfonate, sodium butylnaphthalenesulfonate, lauryl alcohol polyethylene glycol oxide base ether, lauryl sodium sulfate, Tea Saponin, alkylphenol-polyethenoxy base ether, pull open powder, neopelex, Negel, agriculture breast 600#, alkylaryl polyoxyethylene polyoxypropylene ether, fatty alcohol-polyoxyethylene ether, calcium dodecyl benzene sulfonate, SOPA-270, triphen ethyl phenol polyoxyethylene ether phosphate amine salt, thermally coupled distillation columns, methyl naphthalene sulfonate formaldehyde condensate, styrylphenol polyoxyethylene ether, polyoxyethylene nonylphenol ether.

Binding agent is polyvinyl alcohol, starch, carboxymethyl cellulose, ethyl cellulose, Magnesiumaluminumsilicate.

Disintegrant is sodium carbonate, sodium sulphate, ammonium sulfate, ammonium chloride.

Antifreezing agent is ethylene glycol, glycerine, isopropyl alcohol.

Thickener is xanthans, gum Arabic, urea.

Bactericide of the present invention can the multiple formulation in preparation cost field as missible oil, ultra low volume liquids etc.As preferably, in embodiment provided by the invention, bactericide can be prepared into following formulation: wettable powder, water dispersible granules, suspending agent, granule.

Bactericide of the present invention may be used for preventing and treating the Disease on multiple sections genus crops such as tobacco virus, tomato virus disease, cucumber virus disease, oryza virus 3 disease, rice dwarf virus disease viral disease, MRDV viral disease, rice black-streaked dwarf virus disease.May be used for preventing and treating cucumber downy mildew, rice blast, tomato late blight, banana spike rot, Muskmelon Fusarium wilt, citrus anthracnose, grape powdery mildew, corn northern and southern leaf blight, stinking smut and Solanaceae, ground family crop simultaneously and found fungal disease in the various crop such as withered damping off.

Compared with prior art, composition pesticide of the present invention and bactericide tool have the following advantages:

1, malicious fluorine phosphorus and imidazoles bactericide are in (60 ~ 1): after composite in (1 ~ 30) scope, the co-toxicity coefficient of controlling disease is all greater than 120, has obvious synergistic function.

2, effect during bactericidal agent for preventing and treating disease of the present invention, improving drug effect, reduce costs, go back delaying drug resistance simultaneously, reduce chemical pesticide consumption.

Embodiment

Embodiment prepared by preparation:

One, the preparation of water dispersible granules

Press the formula rate of routine each embodiment, each component is mixed, after airslide disintegrating mill is pulverized, stir 30min, mediate, then add in fluid bed and carry out granulation, the water dispersible granules dry after granulation well, namely screening obtains bactericide of the present invention.

Two, the preparation of wettable powder

Malicious fluorine phosphorus, imidazoles bactericide (abbreviation B component), wetting agent, dispersant, filler are mixed, after cracker is pulverized and mixed, the wettable powder of obtained bactericide of the present invention.

Three, the preparation of suspending agent

Malicious fluorine phosphorus, imidazoles bactericide (abbreviation B component), auxiliary agent and water are ground in sand mill, after having ground, in suction high-speed shearing machine, add antifreezing agent, thickener, surplus water mixing carry out high speed shear, sheared the suspending agent of rear obtained bactericide of the present invention.

Four, the preparation of granule

Malicious fluorine phosphorus, imidazoles bactericide (abbreviation B component), auxiliary agent, binding agent, disintegrant, filler are mixed; pulverize, stir after adding water-wet, then use screw extruder pelletizer granulation; sieve after drying, obtain the granule of bactericide of the present invention.

BIOLOGICAL ACTIVITY EXAMPLES

In order to the control efficiency of clear and definite malicious fluorine phosphorus and the bactericide compounded use of imidazoles, inventors performed Toxicity Determination and drug effect test of field zone.

Indoor bioassay is tested:

Composition pesticide of the present invention, combines between them and has obvious synergistic function to different diseases, and be not only the simple addition of two kinds of pharmacy effects, is specifically illustrated with following Assays Example.

Toxicity Determination:

Below raw test is tested and is adopted Sun Yun abundant method calculating co-toxicity coefficient (CTC) to evaluate mixed effect.

Toxicity index TI (B)=(EC of standard agent A ₅₀the EC of ÷ B agent ₅₀) × 100

Actual toxicity index ATI (AB)=(EC of A ₅₀the EC of ÷ AB ₅₀) × 100

Theoretical toxicity index TTI (AB)=TI (the A) × A percentage+TI (B) in the mixture × percentage of B in mixture

Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI) ÷ mixture] × 100

Evaluation criterion: (CTC) >=120 show as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.

Toxicity Determination one:

Test method: raise the malicious plant hopper of band before test.Use polypots nursery, the strong seedling that every alms bowl retains 10 strain neat and consistent is used for connecing worm.Inoculate when rice shoot grows to 1 leaf 1 heart ~ 2 leaf 1 heart, every alms bowl cover is with translucent cover, and top gauze seals, and calculating by every seedling 5 small brown rice planthoppers needs worm amount, accesses for 2 ~ 3 small brown rice planthopper in ages (being with malicious rate about 40%).Expelling parasite every day 2 times, makes tested rice seedling evenly by poison.The liquid of preparation respective concentration, evenly spray above for subsequent use by malicious rice seedling, often process and repeat for 4 times, the rice seedling of each process grows morbidity under natural lighting condition, test establish not containing the process of medicament as blank, wait the blank incidence of disease the to reach state of an illness that 50% time stage investigates each process, utilizes DPS data processing software and Excel, set up the virulence regression equation of each medicament, calculate EC ₅₀value, and calculate co-toxicity coefficient by the abundant method of Sun Yun.Measurement result is in Table 1-3.

The Toxicity Determination result of table 1 water prevention rice black streak dwarf

Composition	EC 50(μg/ml)	ATI	TTI	CTC
					Poison fluorine phosphorus (A)	36.46	100.00	/	/
Cyazofamid (B)	73.52	49.59	/	/
					A:B＝60:1	29.82	122.27	99.17	123.29
A:B＝50:1	27.68	131.72	99.01	133.03

A:B＝40:1	26.18	139.27	98.77	141.00
					A:B＝30:1	25.56	142.64	98.37	145.00
A:B＝20:1	24.76	147.25	97.60	150.88
					A:B＝10:1	22.89	159.28	95.42	166.93
A:B＝5:1	21.35	170.77	91.60	186.44
					A:B＝3:1	19.66	185.45	87.40	212.19
A:B＝2:1	18.88	193.11	83.20	232.12
					A:B＝1:1	21.35	170.77	74.80	228.32
A:B＝1:2	24.34	149.79	66.39	225.61
					A:B＝1:3	26.87	135.69	62.19	218.17
A:B＝1:5	31.83	114.55	57.99	197.52
					A:B＝1:10	42.84	85.11	54.17	157.10
A:B＝1:20	51.32	71.04	51.99	136.64
					A:B＝1:30	58.81	62.00	51.22	121.04
A:B＝1:40	63.37	57.54	50.82	113.21

The Toxicity Determination result of table 2 water prevention rice black streak dwarf

Composition	EC 50(μg/ml)	ATI	TTI	CTC
					Poison fluorine phosphorus (A)	35.91	100.00	/	/
Prochloraz (C)	33.77	106.34	/	/
					Fluorine bacterium azoles (D)	72.91	49.25	/	/
A:C＝60:1	28.53	125.87	100.10	125.74
					A:C＝50:1	26.28	136.64	100.12	136.47
A:C＝40:1	24.86	144.45	100.15	144.23
					A:C＝30:1	24.01	149.56	100.20	149.26
A:C＝20:1	23.45	153.13	100.30	152.67
					A:C＝10:1	21.57	166.48	100.58	165.53

A:C＝5:1	18.73	191.72	101.06	189.72
					A:C＝2:1	16.34	219.77	102.11	215.22
A:C＝1:1	17.57	204.38	103.17	198.11
					A:C＝1:2	20.03	179.28	104.22	172.01
A:C＝1:5	21.04	170.67	105.28	162.11
					A:C＝1:10	22.58	159.03	105.76	150.37
A:C＝1:20	24.55	146.27	106.04	137.95
					A:C＝1:30	27.51	130.53	106.13	122.99
A:C＝1:40	30.38	118.20	106.18	111.32
					A:D＝80:1	32.77	109.58	99.37	110.27
A:D＝60:1	29.64	121.15	99.17	122.17
					A:D＝40:1	25.75	139.46	98.76	141.20
A:D＝30:1	24.37	147.35	98.36	149.81
					A:D＝20:1	23.18	154.92	97.58	158.75
A:D＝10:1	21.66	165.79	95.39	173.81
					A:D＝5:1	18.93	189.70	91.54	207.23
A:D＝2:1	17.82	201.52	83.08	242.54
					A:D＝1:1	21.59	166.33	74.63	222.88
A:D＝1:2	26.49	135.56	66.17	204.87
					A:D＝1:5	32.75	109.65	57.71	190.00
A:D＝1:10	43.72	82.14	53.87	152.48
					A:D＝1:20	49.97	71.86	51.67	139.08
A:D＝1:30	56.88	63.13	50.89	124.06

The Toxicity Determination result of table 3 water prevention rice black streak dwarf

Composition	EC 50(μg/ml)	ATI	TTI	CTC
					Poison fluorine phosphorus (A)	34.89	100.00	/	/
Pefurazoate (E)	66.52	52.45	/	/
					A:E＝60:1	28.27	123.42	99.22	124.39
A:E＝45:1	25.65	136.02	98.97	137.44
					A:E＝30:1	24.24	143.94	98.47	146.18
A:E＝20:1	23.36	149.36	97.74	152.82
					A:E＝10:1	19.83	175.95	95.68	183.89
A:E＝5:1	18.73	186.28	92.08	202.31
					A:E＝2:1	17.34	201.21	84.15	239.11
A:E＝1:1	19.12	182.48	76.23	239.39
					A:E＝1:2	22.82	152.89	68.30	223.85
A:E＝1:3	25.34	137.69	64.34	214.01
					A:E＝1:5	30.31	115.11	60.38	190.66
A:E＝1:10	40.61	85.91	56.77	151.33
					A:E＝1:20	47.83	72.95	54.71	133.32
A:E＝1:30	53.28	65.48	53.98	121.30
					A:E＝1:40	58.76	59.38	53.61	110.76

From the measurement result of table 1-3, poison fluorine phosphorus and imidazoles bactericide are (60 ~ 1) in weight ratio: composite in (1 ~ 30) scope, the co-toxicity coefficient (CTC) of water prevention rice black streak dwarf is all greater than 120, has obvious synergistic function.Especially when weight ratio is in (20 ~ 1): composite in (1 ~ 10) scope, the co-toxicity coefficient (CTC) of water prevention rice black streak dwarf is all greater than 150, and synergy is more remarkable.

Toxicity Determination two:

Test method: indoor pot potato susceptible variety plant, to the 4-5 leaf phase, with the isopyknic viral juice frictional inoculation of liquid mixing of different ratio variable concentrations, often processes 3 strains, often to select good strains in the field for seed 3 leaves, repeat 4 times, 3-4 days " Invest, Then Investigate " withered spot numbers, calculate inhibiting rate and EC ₅₀value, calculate co-toxicity coefficient by the abundant method of Sun Yun, measurement result is in Table 4-5.

The Toxicity Determination result of potato virus disease prevented and treated by table 4

Composition	EC 50(μg/ml)	ATI	TTI	CTC
					Poison fluorine phosphorus (A)	44.69	100.00	/	/
Imazalil (F)	113.62	39.33	/	/
					Fenamidone (G)	103.85	43.03	/	/
A:F＝60:1	37.18	120.20	99.01	121.41
					A:F＝40:1	34.43	129.80	98.52	131.75
A:F＝20:1	29.02	154.00	97.11	158.58
					A:F＝10:1	29.54	151.29	94.48	160.12
A:F＝5:1	28.33	157.75	89.89	175.49
					A:F＝3:1	30.34	147.30	84.83	173.63
A:F＝2:1	26.98	165.64	79.78	207.63
					A:F＝1:1	28.14	158.81	69.67	227.96
A:F＝1:2	34.63	129.05	59.56	216.69
					A:F＝1:5	48.61	91.94	49.44	185.94
A:F＝1:10	63.15	70.77	44.85	157.80
					A:F＝1:20	75.12	59.49	42.22	140.90
A:F＝1:30	88.08	50.74	41.29	122.88
					A:F＝1:40	100.95	44.27	40.81	108.47
A:G＝80:1	41.34	108.10	99.30	108.87
					A:G＝60:1	37.21	120.10	99.07	121.23
A:G＝40:1	32.32	138.27	98.61	140.22
					A:G＝20:1	29.75	150.22	97.29	154.41
A:G＝10:1	27.23	164.12	94.82	173.08
					A:G＝5:1	26.56	168.26	90.51	185.91
A:G＝2:1	28.39	157.41	81.01	194.31
					A:G＝1:1	28.16	158.70	71.52	221.91
A:G＝1:2	34.06	131.21	62.02	211.55

A:G＝1:3	38.06	117.42	57.27	205.01
					A:G＝1:5	43.31	103.19	52.53	196.44
A:G＝1:10	59.29	75.38	48.21	156.34
					A:G＝1:20	65.54	68.19	45.75	149.06
A:G＝1:30	79.67	56.09	44.87	125.01

The Toxicity Determination result of potato virus disease prevented and treated by table 5

Composition	EC 50(μg/ml)	ATI	TTI	CTC
					Poison fluorine phosphorus (A)	45.65	100.00	/	/
Cyazofamid (B)	98.78	46.21	/	/
					A:B＝60:1	37.39	122.09	99.12	123.18
A:B＝40:1	33.75	135.26	98.69	137.06
					A:B＝20:1	30.33	150.51	97.44	154.47
A:B＝10:1	29.46	154.96	95.11	162.92
					A:B＝5:1	28.92	157.85	91.04	173.39
A:B＝4:1	27.23	167.65	89.24	187.85
					A:B＝2:1	26.45	172.59	82.07	210.29
A:B＝1:1	26.92	169.58	73.11	231.96
					A:B＝1:2	31.91	143.06	64.14	223.03
A:B＝1:4	40.64	112.33	56.97	197.17
					A:B＝1:8	49.24	92.71	52.19	177.64
A:B＝1:10	58.41	78.15	51.10	152.93
					A:B＝1:15	65.89	69.28	49.58	139.75
A:B＝1:20	70.38	64.86	48.78	132.98
					A:B＝1:30	74.94	60.92	47.95	127.04

From the measurement result of table 4-5, poison fluorine phosphorus and imidazoles bactericide are (60 ~ 1) in weight ratio: composite in (1 ~ 30) scope, the co-toxicity coefficient (CTC) of control potato virus disease is all greater than 120, has obvious synergistic function.Especially when weight ratio is in (20 ~ 1): composite in (1 ~ 10) scope, the co-toxicity coefficient (CTC) of control potato virus disease is all greater than 150, and synergy is more remarkable.

Toxicity Determination three:

Test method: with reference to " NYT 1156.7-2006 " and " NY/T 1156.6-2006 ", calculate the EC of each medicament ₅₀, and the co-toxicity coefficient of mixture is calculated according to the abundant method of Sun Yun.Measurement result is in Table 6-9.

The Toxicity Determination result of cucumber downy mildew prevented and treated by table 6

Composition	EC 50(μg/ml)	ATI	TTI	CTC
					Poison fluorine phosphorus (A)	116.38	100.00	/	/
Cyazofamid (B)	1.44	8065.14	/	/
					A:B＝60:1	40.57	286.86	230.58	124.41
A:B＝45:1	33.61	346.27	273.16	126.77
					A:B＝30:1	23.88	487.35	356.94	136.54
A:B＝20:1	15.94	730.11	479.29	152.33
					A:B＝10:1	7.23	1609.68	824.10	195.33
A:B＝4:1	2.88	4040.97	1693.03	238.68
					A:B＝2:1	1.68	6927.38	2755.05	251.44
A:B＝1:1	1.38	8433.33	4082.57	206.57
					A:B＝1:2	0.91	12789.01	5410.09	236.39
A:B＝1:5	0.87	13377.01	6737.62	198.54
					A:B＝1:8	0.96	12122.92	7180.13	168.84
A:B＝1:10	1.03	11299.03	7341.04	153.92
					A:B＝1:20	1.15	10120.00	7685.85	131.67
A:B＝1:30	1.24	9385.48	7808.20	120.20
					A:B＝1:40	1.31	8883.97	7870.87	112.87

The Toxicity Determination result of cucumber downy mildew prevented and treated by table 7

Composition	EC 50(μg/ml)	ATI	TTI	CTC
					Poison fluorine phosphorus (A)	104.63	100.00	/	/
Prochloraz (C)	0.86	12166.28	/	/
					Imazalil (F)	1.37	7637.23	/	/
A:C＝60:1	27.94	374.48	297.81	125.75
					A:C＝40:1	19.28	542.69	394.30	137.63
A:C＝20:1	10.13	1032.87	674.58	153.11
					A:C＝10:1	5.42	1930.44	1196.93	161.28
A:C＝5:1	2.85	3671.23	2111.05	173.91
					A:C＝3:1	1.90	5506.84	3116.57	176.70
A:C＝2:1	1.36	7693.38	4122.09	186.64
					A:C＝1:1	0.79	13244.30	6133.14	215.95
A:C＝1:2	0.58	18039.66	8144.19	221.50
					A:C＝1:5	0.55	19023.64	10155.23	187.33
A:C＝1:10	0.61	17152.46	11069.34	154.95
					A:C＝1:20	0.61	17152.46	11591.69	147.97
A:C＝1:30	0.73	14332.88	11777.04	121.70
					A:C＝1:40	0.82	12759.76	11871.98	107.48
A:F＝80:1	45.57	229.60	193.05	118.93
					A:F＝60:1	38.61	270.99	223.56	121.22
A:F＝40:1	27.88	375.29	283.83	132.22
					A:F＝20:1	14.94	700.33	458.92	152.61
A:F＝10:1	7.23	1447.16	785.20	184.30
					A:F＝5:1	3.88	2696.65	1356.20	198.84
A:F＝2:1	1.68	6227.98	2612.41	238.40
					A:F＝1:1	1.18	8866.95	3868.61	229.20
A:F＝1:2	0.91	11497.80	5124.82	224.36

A:F＝1:3	0.87	12026.44	5752.92	209.05
					A:F＝1:5	0.96	10898.96	6381.02	170.80
A:F＝1:10	0.97	10786.60	6952.02	155.16
					A:F＝1:20	1.13	9259.29	7278.31	127.22
A:F＝1:30	1.17	8942.74	7394.09	120.94

The Toxicity Determination result of cucumber downy mildew prevented and treated by table 8

Composition	EC 50(μg/ml)	ATI	TTI	CTC
					Poison fluorine phosphorus (A)	105.77	100.00	/	/
Fluorine bacterium azoles (D)	1.16	9118.10	/	/
					Fenamidone (G)	1.07	9885.05	/	/
A:D＝60:1	34.46	306.94	247.84	123.85
					A:D＝40:1	23.81	444.23	319.95	138.84
A:D＝20:1	12.65	836.13	529.43	157.93
					A:D＝10:1	6.94	1524.06	919.83	165.69
A:D＝5:1	3.37	3138.58	1603.02	195.79
					A:D＝2:1	1.58	6694.30	3106.03	215.53
A:D＝1:1	1.11	9528.83	4609.05	206.74
					A:D＝1:2	0.91	11623.08	6112.07	190.17
A:D＝1:5	0.87	12157.47	7615.09	159.65
					A:D＝1:10	0.83	12743.37	8298.28	153.57
A:D＝1:20	0.93	11373.12	8688.67	130.90
					A:D＝1:30	0.99	10683.84	8827.20	121.03
A:D＝1:40	1.04	10170.19	8898.15	114.30
					A:G＝80:1	40.57	260.71	220.80	118.07
A:G＝60:1	32.61	324.35	260.41	124.55

A:G＝40:1	21.88	483.41	338.66	142.74
					A:G＝20:1	11.94	885.85	565.95	156.52
A:G＝10:1	6.23	1697.75	989.55	171.57
					A:G＝5:1	2.98	3549.33	1730.84	205.06
A:G＝2:1	1.44	7345.14	3361.68	218.50
					A:G＝1:1	0.88	12019.32	4992.52	240.75
A:G＝1:2	0.68	15554.41	6623.36	234.84
					A:G＝1:3	0.71	14897.18	7438.79	200.26
A:G＝1:5	0.72	14690.28	8254.21	177.97
					A:G＝1:10	0.76	13917.11	8995.50	154.71
A:G＝1:20	0.81	13058.02	9419.09	138.63
					A:G＝1:30	0.87	12157.47	9569.40	127.05

The Toxicity Determination result of cucumber downy mildew prevented and treated by table 9

Composition	EC 50(μg/ml)	ATI	TTI	CTC
					Poison fluorine phosphorus (A)	108.53	100.00	/	/
Pefurazoate (E)	3.29	3298.78	/	/
					A:E＝60:1	57.92	187.38	152.44	122.92
A:E＝40:1	45.06	240.86	178.02	135.30
					A:E＝20:1	28.33	383.09	252.32	151.83
A:E＝10:1	17.39	624.09	390.80	159.70
					A:E＝6:1	8.68	1250.35	633.13	197.49
A:E＝3:1	5.32	2040.04	899.70	226.75
					A:E＝2:1	3.96	2740.66	1166.26	235.00
A:E＝1:1	2.85	3808.07	1699.39	224.08
					A:E＝1:2	2.36	4598.73	2232.52	205.99
A:E＝1:3	2.17	5001.38	2659.03	188.09

A:E＝1:6	2.01	5399.50	2943.36	183.45
					A:E＝1:10	2.38	4560.08	3007.99	151.60
A:E＝1:15	2.46	4411.79	3098.86	142.37
					A:E＝1:20	2.67	4064.79	3146.46	129.19
A:E＝1:30	2.76	3932.25	3195.60	123.05

From the measurement result of table 6-9, poison fluorine phosphorus and imidazoles bactericide are (60 ~ 1) in weight ratio: composite in (1 ~ 30) scope, the co-toxicity coefficient (CTC) of control cucumber downy mildew is all greater than 120, has obvious synergistic function.Especially when weight ratio is in (20 ~ 1): composite in (1 ~ 10) scope, the co-toxicity coefficient (CTC) of control cucumber downy mildew is all greater than 150, and synergy is more remarkable.

Field control effectiveness test:

The controlling disease result of the test that bactericide of the present invention is applied large Tanaka below.Wetting powder is also that wettable powder is called for short WP.

Contrast medicament:

CK1:30% poison fluorine phosphorus wetting powder, registration card LS20130359, Guangxi Tianyuan Biochemical Co., Ltd.;

CK2:100g/L cyazofamid suspending agent, registration card PD20050191, Japanese stone originates in Ye Zhu formula club;

CK3:450g/L prochloraz aqueous emulsion, registration card PD20150070, Zhejiang Tianfeng Biological Science Co., Ltd.;

CK4:30% triflumizole wettable powder, registration card PD20121474, Shaanxi Meibang Pesticide Co., Ltd.;

CK5:20% pefurazoate wettable powder, self-control;

CK6:22.2% Imazalil EC, registration card PD300-99, Xian Nong Co., Ltd of the U.S.;

CK7:20% Fenamidone wettable powder, self-control.

Field efficacy embodiment one:

Test method: with reference to " NY/T 1464.8-2007 pesticide field efficacy medicine test criterion bactericidal agent for preventing and treating tomato virus disease ".Controlling object is black streaked dwarf virus of rice.First time dispenser after rice transplanting 15-20d, at interval of 7d dispenser again, dispenser 2 times altogether.

Investigation method: respectively at 7d, 14d investigation after twice medicine, according to related data investigation standard grading, statistics disease index, calculates preventive effect, the results are shown in Table 10.

The field test results of table 10 water prevention rice black streak dwarf

From the field test results of table 10, the bactericide of toxic fluorine phosphorus of the present invention and imidazoles bactericide pesticide composite, when active ingredient dosage is less than contrast medicament, water prevention rice black streak dwarf is higher by 24% ~ 44% than the preventive effect of contrast medicament, improve control efficiency, and the lasting period is long.

Field efficacy embodiment two:

Test method and drug effect computational methods: with reference to " NY/T 1464.8-2007 pesticide field efficacy medicine test criterion bactericidal agent for preventing and treating tomato virus disease ", controlling object is potato virus disease.First time dispenser after 15-20d after potato planting, at interval of 7d dispenser again, dispenser 2 times altogether.

Investigation method: respectively at 7d, 14d investigation after twice medicine, according to related data investigation standard grading, statistics disease index, calculates preventive effect, the results are shown in Table 11.

The field test results of potato virus disease prevented and treated by table 11

From the field test results of table 11, the bactericide of toxic fluorine phosphorus of the present invention and imidazoles bactericide pesticide composite, when active ingredient dosage is less than contrast medicament, control potato virus disease is higher by 24% ~ 40% than the preventive effect of contrast medicament, improve control efficiency, and the lasting period is long.

Field efficacy embodiment three:

Test method: with reference to " GB/T 17980.26-2000 pesticide field efficacy medicine test criterion (one) bactericidal agent for preventing and treating cucumber downy mildew "; Controlling object is cucumber downy mildew.Each plot area is 20 ㎡, arranges 4 repetitions, altogether dispenser 2 times, 7 days, each dispenser interval.

Investigation method: respectively at 7d, 14d investigation after twice medicine, according to related data investigation standard grading, statistics disease index, calculates preventive effect, the results are shown in Table 15.

The field test results of cucumber downy mildew prevented and treated by table 12

From the field test results of table 12, the bactericide of toxic fluorine phosphorus of the present invention and imidazoles bactericide pesticide composite, when active ingredient dosage is less than contrast medicament, control cucumber downy mildew is higher by 26% ~ 49% than the preventive effect of contrast medicament, improve control efficiency, and the lasting period is long.

Claims (8)

1. a composition pesticide, is characterized in that, described composition pesticide is containing poisonous fluorine phosphorus and imidazoles bactericide; Wherein imidazoles bactericide is selected from one or more in cyazofamid, Prochloraz, fluorine bacterium azoles, pefurazoate, imazalil or Fenamidone; The weight ratio of described malicious fluorine phosphorus and imidazoles bactericide is (60 ~ 1): (1 ~ 30).

2. composition pesticide according to claim 1, is characterized in that, the weight ratio of described malicious fluorine phosphorus and imidazoles bactericide is (20 ~ 1): (1 ~ 10).

3. the bactericide containing the arbitrary described composition pesticide of claim 1-2, it is characterized in that, described composition pesticide accounts for the 0.1-90% of described bactericide gross weight.

4. bactericide according to claim 3, is characterized in that, described composition pesticide accounts for the 6-70% of described bactericide gross weight.

5. bactericide according to claim 3, is characterized in that, described bactericide is also containing acceptable auxiliary element on agricultural chemicals.

6. bactericide according to claim 3, is characterized in that, described bactericide is prepared into following formulation: wettable powder, suspending agent, water dispersible granules or granule.

7. the purposes of bactericide according to claim 3 in control Disease or fungal disease.

8. the purposes of the bactericide according to claim 4-6 in control Disease or fungal disease.