CN104897888A - 一种分隔式酶反应传感器系统 - Google Patents

一种分隔式酶反应传感器系统 Download PDF

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CN104897888A
CN104897888A CN201510274831.2A CN201510274831A CN104897888A CN 104897888 A CN104897888 A CN 104897888A CN 201510274831 A CN201510274831 A CN 201510274831A CN 104897888 A CN104897888 A CN 104897888A
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enzyme reaction
phosphate buffer
flow cell
separated
oxygen electrode
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任磊
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Hefei Zhuoyuan Technology Service Co Ltd
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Hefei Zhuoyuan Technology Service Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

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Abstract

本发明公开了一种分隔式酶反应传感器系统，涉及酶反应传感器领域。为了使酶反应可以分隔开从而测定过程简洁有效，本发明中：磷酸盐缓冲液所在的器皿置于水浴池中，固定抗体反应器通过注入部件、三通活塞与磷酸盐缓冲液所在的器皿相连，流通池通过三通活塞、蠕动泵与固定抗体反应器相连，氧电极位于流通池内，氧电极与安培计相连，安培计与记录器相连。本发明采用了分子识别元件和转换器完全分开的方式，使酶反应可以分隔开，使得测定过程简洁有效。

Description

一种分隔式酶反应传感器系统
技术领域
    本发明涉及酶反应传感器领域，尤其涉及一种分隔式酶反应传感器系统。
背景技术
自从免疫传感器问世以来，许多生物有机物质在以前是无法测量的，现在逐一得到解决。免疫传感器是利用抗体与相应抗原的识别功能和结合功能而设计的一种检测装置，利用这种原理设计出来的装置或系统可对许多有机物进行检测。
发明内容
本发明要解决以上的技术问题，提供一种分隔式酶反应传感器系统，使酶反应可以分隔开从而测定过程简洁有效。
为解决上述技术问题，本发明是通过以下技术方案实现的：
一种分隔式酶反应传感器系统，包括水浴池、磷酸盐缓冲液、三通活塞、注入部件、固定抗体反应器、蠕动泵、流通池、氧电极、安培计、记录器，磷酸盐缓冲液所在的器皿置于水浴池中，固定抗体反应器通过注入部件、三通活塞与磷酸盐缓冲液所在的器皿相连，流通池通过三通活塞、蠕动泵与固定抗体反应器相连，氧电极位于流通池内，氧电极与安培计相连，安培计与记录器相连。
与现有的技术相比，本发明的有益效果是：本发明采用了分子识别元件和转换器完全分开的方式，使酶反应可以分隔开，使得测定过程简洁有效。
附图说明
图1为本发明的原理结构示意图；
其中：1、水浴池，2、氮，3、磷酸盐缓冲液，4、三通活塞，5、注入部件，6、固定抗体反应器，7、蠕动泵，8、流通池，9、氧电极，10、安培计，11、记录器。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合附图及实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。
请参阅图1，图1为本发明的原理结构示意图。
本发明提供一种分隔式酶反应传感器系统，包括水浴池、磷酸盐缓冲液、三通活塞、注入部件、固定抗体反应器、蠕动泵、流通池、氧电极、安培计、记录器，磷酸盐缓冲液所在的器皿置于水浴池中，固定抗体反应器通过注入部件、三通活塞与磷酸盐缓冲液所在的器皿相连，流通池通过三通活塞、蠕动泵与固定抗体反应器相连，氧电极位于流通池内，氧电极与安培计相连，安培计与记录器相连。
以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等，均应包含在本发明的保护范围之内。

Claims (1)

1.一种分隔式酶反应传感器系统，包括水浴池、磷酸盐缓冲液、三通活塞、注入部件、固定抗体反应器、蠕动泵、流通池、氧电极、安培计、记录器，其特征在于，磷酸盐缓冲液所在的器皿置于水浴池中，固定抗体反应器通过注入部件、三通活塞与磷酸盐缓冲液所在的器皿相连，流通池通过三通活塞、蠕动泵与固定抗体反应器相连，氧电极位于流通池内，氧电极与安培计相连，安培计与记录器相连。
CN201510274831.2A 2015-05-27 2015-05-27 一种分隔式酶反应传感器系统 Pending CN104897888A (zh)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010045359A1 (en) * 1996-09-06 2001-11-29 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
CN101003830A (zh) * 2006-12-31 2007-07-25 华东师范大学 快速计数水体中的大肠杆菌的方法
CN101021529A (zh) * 2007-03-26 2007-08-22 南京大学 多分析物同时检测的高通量检测系统及电化学免疫分析方法
US20090169428A1 (en) * 2005-07-13 2009-07-02 Sean Anthony Gillespie Flow Cell With Piezoelectric Ultrasonic Transducer
CN103412025A (zh) * 2013-07-23 2013-11-27 合肥工业大学 基于流动注射分析技术的葡萄糖浓度检测装置及检测方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010045359A1 (en) * 1996-09-06 2001-11-29 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US20090169428A1 (en) * 2005-07-13 2009-07-02 Sean Anthony Gillespie Flow Cell With Piezoelectric Ultrasonic Transducer
CN101003830A (zh) * 2006-12-31 2007-07-25 华东师范大学 快速计数水体中的大肠杆菌的方法
CN101021529A (zh) * 2007-03-26 2007-08-22 南京大学 多分析物同时检测的高通量检测系统及电化学免疫分析方法
CN103412025A (zh) * 2013-07-23 2013-11-27 合肥工业大学 基于流动注射分析技术的葡萄糖浓度检测装置及检测方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
IHAB ABDEL-HAMID ET AL.: "Highly sensitive flow-injection immunoassay system for rapid detection of bacteria", 《ANALYTICA CHIMICA ACTA》 *
LAURIE LOCASCIO-BROWN ET AL.: "Liposome Flow Injection Immunoassay: Implications for Sensitivity, Dynamic Range, and Antibody Regeneration", 《ANAL. CHEM.》 *

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