CN104892700A - Method for extracting anthocyanin from cherries - Google Patents
Method for extracting anthocyanin from cherries Download PDFInfo
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- CN104892700A CN104892700A CN201510318509.5A CN201510318509A CN104892700A CN 104892700 A CN104892700 A CN 104892700A CN 201510318509 A CN201510318509 A CN 201510318509A CN 104892700 A CN104892700 A CN 104892700A
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- cherry
- anthocyanogen
- wine
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Abstract
The invention discloses a method for extracting anthocyanin from cherries. The method has the advantages of simple process, comprehensive utilization of raw materials, more byproducts, high yield, low cost and application to large scale production; the extract is rich in anthocyanin and procyanidine type components, has an excellent biological activity and pharmacological activities on aspects of treating diabetes, resisting imflammation, resisting cancers, preventing and treating cardiovascular diseases, and is applicable to the field of foods, health products, pharmaceuticals and the like.
Description
Technical field
The present invention relates to the extracting method of anthocyanogen in cherry, this extract can be applicable to the fields such as food, healthcare products, medicine.
Background technology
Cherry is that Rosaceae machaka fruit tree is also called car li son, and not only edibleness is very high, also has taste sweet warm in nature as Chinese medicine, has the function such as tonifying Qi, wind-damp dispelling in tune.And working hard property of Nux Pruni pseudocerasi taste is put down, and has the function of removing toxic substances.
In prior art, mainly from cherry, extract VC.Have the technique extracting anthocyanogen in cherry, many employing water extractions or alcohol extracting method, product is single only has anthocyanogen.And fermentation method extracts anthocyanogen from cherry can obtain cherry anthocyanogen and cherry liquor two kinds of products simultaneously.
The U.S. has investigator from cherry, to extract anthocyanogen recently, and in rodent body, take out the pancreatic cell manufacturing Regular Insulin, detects anthocyanogen to the effect of these cells.After contact anthocyanogen, the Regular Insulin generation of cell can raise half.Illustrate that the anthocyanogen in cherry has certain curative effect to diabetes.Cherry does not belong to medicine, and the anthocyanogen extracted can not develop immunity to drugs to human body, is a kind of ideal, effective food, healthcare products, the drug additive controlling diabetes.
Summary of the invention
The object of the present invention is to provide the extracting method of anthocyanogen in a kind of cherry, the method has that technique is simple, comprehensive utilization raw material, product is many, yield is high, cost is low, be suitable for the advantage of large-scale production; Be rich in anthocyanogen and pycnogenols constituents in extract obtained, in treatment diabetes, anti-inflammatory, anticancer, prevention and cure of cardiovascular disease etc., there is good biological activity and pharmacological action, be applicable to the fields such as food, healthcare products, medicine.
In order to achieve the above object, the present invention is achieved by the following technical solutions.
Extract a method for anthocyanogen in cherry, it is characterized in that, comprise the following steps:
(1), by cherry fruit break process, after adding biological enzyme enzymolysis, ferment under suitable fermentation condition, obtain the former wine of cherry;
(2) more former for cherry wine is put into underpressure distillation pot, distillation 1h, collects fragrance matter, obtains cherry liquor and anthocyanogen crude extract;
(3), by anthocyanogen crude extract, by absorption with macroporous adsorbent resin, after washing macroporous adsorbent resin with water, resolve with alcoholic solution, obtain desorbed solution;
(4) by desorbed solution cryoconcentration, vacuum decompression drying, obtain cherry anthocyanogen essence and carry powder.
Feature of the present invention is also:
In step (1), described biological enzyme be polygalacturonase, cellulase, xylosidase, saccharifying enzyme one or more; During enzymolysis, in solution, enzyme lives requirement more than 5000 units;
Fermentation condition suitable in described step (1), its leavening temperature is 20 ~ 30 DEG C, and fermentation time is 18 days, and inoculum of dry yeast is 0.1 ‰ ~ 0.5 ‰, and the adjustment of fermented liquid pH value can adopt citric acid or tartrate.
Underpressure distillation in described step (2), its pressure is-0.01mpa ~-0.1 mpa, and Heating temperature, with going out the change of wine situation, is specially 30 ~ 60 DEG C.
In described step (3), macroporous adsorbent resin is nonpolar or low-pole resin.
In described step (4), the cryoconcentration method of desorbed solution is normal pressure or reclaim under reduced pressure method, and temperature is, 30 60 ° of C, and concentrated relative density is 1. 0 1. 2; Its drying means is that 140 180 ° of C spraying dry or 20 60 ° of C vacuum decompressions are dry.
The present invention is the extracting method of anthocyanogen in a kind of cherry, and the method has that technique is simple, comprehensive utilization raw material, product is many, yield is high, cost is low, be suitable for the advantage of large-scale production; Be rich in anthocyanogen and pycnogenols constituents in extract obtained, in treatment diabetes, anti-inflammatory, anticancer, prevention and cure of cardiovascular disease etc., there is good biological activity and pharmacological action, be applicable to the fields such as food, healthcare products, medicine.
Embodiment
The embodiment of extracting method of the present invention comprises the following steps.
(1), by cherry fruit break process, after adding biological enzyme enzymolysis, ferment under suitable fermentation condition, obtain the former wine of cherry; Wherein said biological enzyme be polygalacturonase, cellulase, xylosidase, saccharifying enzyme one or more; During enzymolysis, in solution, enzyme lives requirement more than 5000 units; Suitable fermentation condition, its leavening temperature is 20 ~ 30 DEG C, and fermentation time is 18 days, and inoculum of dry yeast is 0.1 ‰ ~ 0.5 ‰, and the adjustment of fermented liquid pH value can adopt citric acid or tartrate.
(2) more former for cherry wine is put into underpressure distillation pot, distillation 1h, collects fragrance matter, obtains cherry liquor and anthocyanogen crude extract; Underpressure distillation wherein, its pressure is-0.01mpa ~-0.1 mpa, and Heating temperature, with going out the change of wine situation, is specially 30 ~ 60 DEG C.
(3), by anthocyanogen crude extract, by absorption with macroporous adsorbent resin, after washing macroporous adsorbent resin with water, resolve with alcoholic solution, obtain desorbed solution; Macroporous adsorbent resin is nonpolar or low-pole resin.
(4) desorbed solution is concentrated, dry, obtain cherry anthocyanogen essence and carry powder.The cryoconcentration method of desorbed solution is normal pressure or reclaim under reduced pressure method, and temperature is, 30 60 ° of C, and concentrated relative density is 1. 0 1. 2; Its drying means is that 140 180 ° of C spraying dry or 20 60 ° of C vacuum decompressions are dry.
Wherein the gained cherry anthocyanogen essence anthocyanin content carried in powder is 10 70%
Below in conjunction with specific examples, technical solution of the present invention is described in further detail.
Example one, get cherry fresh fruit 5kg, attrition crushing, activated cellulose enzyme under 45 50 ° of C, add that cellulase is fresh fruit weight 1 ‰, adjust pH value to 3.5 with citric acid, sugared addition is 15%, and inoculum of dry yeast is 0.2 ‰, leavening temperature is 25 ~ 27 DEG C, fermentation time is 5d, more former for cherry wine is put into underpressure distillation pot, distillation 1h, pressure, for-0.05 mpa Heating temperature is with going out the change of wine situation, is specially 30 ~ 60 DEG C.Collect fragrance matter, obtain cherry liquor and anthocyanogen crude extract.Cherry liquor is recycled, and by anthocyanogen crude extract, by absorption with macroporous adsorbent resin, after washing macroporous adsorbent resin with water, resolves, obtain desorbed solution with alcoholic solution; After desorbed solution being evaporated to relative density 1. 1,40 ° of C vacuum decompressions are dry, and must carry powder 22.1g containing anthocyanogen essence, yield is 0. 44% of fresh fruit weight.Obtain 60%(v/v) cherry liquor 503ml.
Example two, get cherry fresh fruit 5kg, attrition crushing, under 45 50 ° of C, activate polygalacturonase, add that polygalacturonase is fresh fruit weight 2 ‰, adjust pH value to 3.0 with tartrate, sugared addition is 15%, and inoculum of dry yeast is 0.3 ‰, leavening temperature is 25 ~ 27 DEG C, fermentation time is 6d, more former for cherry wine is put into underpressure distillation pot, distillation 1h, pressure, for-0.06 mpa Heating temperature is with going out the change of wine situation, is specially 30 ~ 60 DEG C.Collect fragrance matter, obtain cherry liquor and anthocyanogen crude extract.Cherry liquor is recycled, and by anthocyanogen crude extract, by absorption with macroporous adsorbent resin, after washing macroporous adsorbent resin with water, resolves, obtain desorbed solution with alcoholic solution; After desorbed solution being evaporated to relative density 1. 2,45 ° of C vacuum decompressions are dry, and must carry powder 24.2g containing anthocyanogen essence, yield is 0. 48% of fresh fruit weight.Obtain 60%(v/v) cherry liquor 510ml.
Example three, get cherry fresh fruit 5kg, attrition crushing, activated cellulose enzyme and polygalacturonase under 45 50 ° of C, add that cellulase is fresh fruit weight 1 ‰, add that cellulase is fresh fruit weight 2 ‰, adjust pH value to 3.5 with citric acid, sugar addition is 20%, inoculum of dry yeast is 0.25 ‰, and leavening temperature is 27 ~ 30 DEG C, and fermentation time is 4d, again former for cherry wine is put into underpressure distillation pot, distillation 1h, pressure, for-0.06mpa Heating temperature is with going out the change of wine situation, is specially 30 ~ 60 DEG C.Collect fragrance matter, obtain cherry liquor and anthocyanogen crude extract.Cherry liquor is recycled, and by anthocyanogen crude extract, by absorption with macroporous adsorbent resin, after washing macroporous adsorbent resin with water, resolves, obtain desorbed solution with alcoholic solution; After desorbed solution being evaporated to relative density 1. 1,50 ° of C vacuum decompressions are dry, and must carry powder 25.1g containing anthocyanogen essence, yield is 0. 50% of fresh fruit weight.Obtain 60%(v/v) cherry liquor 524ml.
Although be described embodiment of the present invention with above-mentioned example, the present invention is not limited to above-mentioned specific examples, and above-mentioned example is only schematic, guiding, and nonrestrictive.Those of ordinary skill in the art is under the enlightenment of this specification sheets, and when not departing from the claims in the present invention institute protection domain, can also make a variety of form, these all belong to the row of the present invention's protection.
Claims (6)
1. extract a method for anthocyanogen in cherry, it is characterized in that, comprise the following steps:
(1), by cherry fruit break process, after adding biological enzyme enzymolysis, ferment under suitable fermentation condition, obtain the former wine of cherry;
(2) more former for cherry wine is put into underpressure distillation pot, distillation 1h, collects fragrance matter, obtains cherry liquor and anthocyanogen crude extract;
(3), by anthocyanogen crude extract, by absorption with macroporous adsorbent resin, after washing macroporous adsorbent resin with water, resolve with alcoholic solution, obtain desorbed solution;
(4) by desorbed solution cryoconcentration, drying, obtain cherry anthocyanogen essence and carry powder.
2. according to claim 1, in step (1), described biological enzyme be polygalacturonase, cellulase, xylosidase, saccharifying enzyme one or more; During enzymolysis, in solution, enzyme lives requirement more than 5000 units.
3. according to claim 1, fermentation condition suitable in step (1), its leavening temperature is 20 ~ 30 DEG C, and fermentation time is 18 days, and inoculum of dry yeast is 0.1 ‰ ~ 0.5 ‰, and the adjustment of fermented liquid pH value can adopt citric acid or tartrate.
4. according to claim 1, the underpressure distillation in step (2), its pressure is-0.01mpa ~-0.1 mpa, and Heating temperature, with going out the change of wine situation, is specially 30 ~ 60 DEG C.
5., according to claim 1, in step (3), macroporous adsorbent resin is nonpolar or low-pole resin.
6., according to claim 1, in step (4), the cryoconcentration method of desorbed solution is normal pressure or reclaim under reduced pressure method, and temperature is 30 60 ° of C, and concentrated relative density is 1. 0 1. 2; Its drying means is that 140 180 ° of C spraying dry or 20 60 ° of C vacuum decompressions are dry.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510318509.5A CN104892700A (en) | 2015-06-11 | 2015-06-11 | Method for extracting anthocyanin from cherries |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510318509.5A CN104892700A (en) | 2015-06-11 | 2015-06-11 | Method for extracting anthocyanin from cherries |
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| CN104892700A true CN104892700A (en) | 2015-09-09 |
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| CN201510318509.5A Pending CN104892700A (en) | 2015-06-11 | 2015-06-11 | Method for extracting anthocyanin from cherries |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108785411A (en) * | 2018-07-05 | 2018-11-13 | 蔡超 | A kind of Preparation method and use of the cherry powder of control diabetes |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000033824A2 (en) * | 1998-12-11 | 2000-06-15 | Michigan State University | Bioflavonoids, anthocyanins and phenolic compounds from cherries for inhibiting inflammation |
| CN1408401A (en) * | 2001-09-21 | 2003-04-09 | 赵春生 | Cherry extract and its extracting method |
| WO2006111163A1 (en) * | 2005-04-21 | 2006-10-26 | Novozymes A/S | Plant extraction process |
| CN1993135A (en) * | 2004-07-29 | 2007-07-04 | 密执安州大学 | Insulin secretion by anthocyanins and anthocyanidins |
| CN101348758A (en) * | 2008-08-23 | 2009-01-21 | 烟台新时代健康产业有限公司 | Brewing method of pine pollen type cherry wine |
-
2015
- 2015-06-11 CN CN201510318509.5A patent/CN104892700A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000033824A2 (en) * | 1998-12-11 | 2000-06-15 | Michigan State University | Bioflavonoids, anthocyanins and phenolic compounds from cherries for inhibiting inflammation |
| CN1408401A (en) * | 2001-09-21 | 2003-04-09 | 赵春生 | Cherry extract and its extracting method |
| CN1993135A (en) * | 2004-07-29 | 2007-07-04 | 密执安州大学 | Insulin secretion by anthocyanins and anthocyanidins |
| WO2006111163A1 (en) * | 2005-04-21 | 2006-10-26 | Novozymes A/S | Plant extraction process |
| CN101348758A (en) * | 2008-08-23 | 2009-01-21 | 烟台新时代健康产业有限公司 | Brewing method of pine pollen type cherry wine |
Non-Patent Citations (2)
| Title |
|---|
| 于东，等: "花色苷提取、分离纯化及鉴定的研究进展", 《食品与发酵工业》 * |
| 肖军霞，等: "樱桃花色苷的提取及抗氧化活性研究", 《中国食品学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108785411A (en) * | 2018-07-05 | 2018-11-13 | 蔡超 | A kind of Preparation method and use of the cherry powder of control diabetes |
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