CN104880461A - Acid-base indicator based on natural plant pigment and preparation method thereof - Google Patents
Acid-base indicator based on natural plant pigment and preparation method thereof Download PDFInfo
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- CN104880461A CN104880461A CN201510344141.XA CN201510344141A CN104880461A CN 104880461 A CN104880461 A CN 104880461A CN 201510344141 A CN201510344141 A CN 201510344141A CN 104880461 A CN104880461 A CN 104880461A
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- 239000002696 acid base indicator Substances 0.000 title claims abstract description 30
- 239000000049 pigment Substances 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000000284 extract Substances 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 8
- 238000000605 extraction Methods 0.000 claims abstract description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 30
- 241000196324 Embryophyta Species 0.000 claims description 9
- 235000009836 Prunus pissardii Nutrition 0.000 claims description 7
- 244000061458 Solanum melongena Species 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 238000004040 coloring Methods 0.000 claims description 2
- 241001506873 Prunus cerasifera Species 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 5
- 230000000869 mutational effect Effects 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- WXLFIFHRGFOVCD-UHFFFAOYSA-L azophloxine Chemical compound [Na+].[Na+].OC1=C2C(NC(=O)C)=CC(S([O-])(=O)=O)=CC2=CC(S([O-])(=O)=O)=C1N=NC1=CC=CC=C1 WXLFIFHRGFOVCD-UHFFFAOYSA-L 0.000 abstract 1
- FUKUFMFMCZIRNT-UHFFFAOYSA-N hydron;methanol;chloride Chemical compound Cl.OC FUKUFMFMCZIRNT-UHFFFAOYSA-N 0.000 abstract 1
- 239000003440 toxic substance Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 8
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 7
- 238000004448 titration Methods 0.000 description 7
- 244000277586 Prunus pissardii Species 0.000 description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 description 4
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 2
- RWZYAGGXGHYGMB-UHFFFAOYSA-N anthranilic acid Chemical compound NC1=CC=CC=C1C(O)=O RWZYAGGXGHYGMB-UHFFFAOYSA-N 0.000 description 2
- 235000007336 cyanidin Nutrition 0.000 description 2
- VEVZSMAEJFVWIL-UHFFFAOYSA-O cyanidin cation Chemical compound [O+]=1C2=CC(O)=CC(O)=C2C=C(O)C=1C1=CC=C(O)C(O)=C1 VEVZSMAEJFVWIL-UHFFFAOYSA-O 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- 244000000383 Allium odorum Species 0.000 description 1
- 235000018645 Allium odorum Nutrition 0.000 description 1
- 244000078782 Brassica arvensis Species 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 241000220299 Prunus Species 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000035613 defoliation Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003317 industrial substance Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- STZCRXQWRGQSJD-GEEYTBSJSA-M methyl orange Chemical compound [Na+].C1=CC(N(C)C)=CC=C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 STZCRXQWRGQSJD-GEEYTBSJSA-M 0.000 description 1
- 229940012189 methyl orange Drugs 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses an acid-base indicator based on natural plant pigment. The acid-base indicator based on the natural plant pigment is characterized in that the natural plant pigment extracted from purple-leaf plum leaves serves as the indicator. A preparation method includes the steps that the purple-leaf plum leaves are collected, washed with flowing water, aired, then subjected to vacuum freeze drying at minus 1-minus 25 DEG C and 10-1000 Pa, ground into powder, and kept out of sun at 25-40 DEG C; the obtained powder is subjected to extraction 2-5 times with hydrochloric acid methanol, the extract liquor is combined and kept out of sun at 0-4 DEG C, and the extract liquor can serve as the acid-base indicator. The acid-base indicator based on the natural plant pigment has the advantages that the pH mutational range of the acid-base indicator is narrow and is within the range of 5.5-6.5, and the acid-base indicator is sensitive to pH changes; the color changes obviously and changes from acid bright red to base yellow green, so that the finishing point is judged easily; all the raw materials in the production or use process are natural plant or low-toxic chemical materials, and the products and the production process are environmentally friendly; the source of the raw materials is wide, the preparation process is simple, and the acid-base indicator based on the natural plant pigment can be produced on a large scale.
Description
Technical field
The invention belongs to natural plant pigment preparation method and application, be specifically related to one and from prunus cerasifera, extract natural plant pigment as acid base indicator and preparation method thereof.
Background technology
Acid base indicator is the conventional chemical reagent of test solution Acidity of Aikalinity, comprises phenolphthalein, methyl orange, methyl red etc.Wherein, methyl red formal name used at school is butter yellow o-carboxylic acid, as a kind of common indicator of strong acid titration weak base, is widely used in inorganic and analytical chemistry.
But at present, when methyl red is applied as acid base indicator, there are the following problems: (1) mutational range is wide, color change is not remarkable, and error at measurment is large.Methyl red is 4.4-6.2 as the color change interval of indicator, the pH of nearly 2 units of span; Meanwhile, when solution becomes alkalescence from acidity, it becomes the yellow of alkali formula from the redness of acid, and human eye is not obvious to reddish yellow sudden change, causes like this, when applying methyl red as indicator, analyzing error at measurment larger.(2) complicated process of preparation, preparation process is large to environmental impact.Be in particular in methyl red preparation usually with the radioactive substance such as ortho-aminobenzoic acid and sodium nitrite for raw material, the organic solvent of, strong toxicity volatile with toluene etc. is for extraction agent, need through the complicated chemical process such as diazotising, coupling, organic solvent washing and recrystallization simultaneously, the waste water produced in preparation process, discarded object not only cause larger environmental pollution, and multiple toxic raw material may cause damage to operative employee is healthy.Therefore, the acid base indicator of the alternative methyl red finding a kind of variable color sensitive, environmental friendliness, wide material sources is needed badly.
In recent years; (CN 102213679 A, CN 103149322 A, CN 101788496 A, CN 101672798 A, CN 1796986 A) is reported about from extracting pigment in petunia petal, fragrant-flowered garlic lotus fruit, violet cabbage etc. as the research of acid base indicator although have; but exist raw material sources few, not easily obtain; the problem such as particularly color change is not obvious, causes being difficult to apply.
Summary of the invention
The object of this invention is to provide a kind of environmental protection, the change of pH change instruction sensitive, color significantly based on the acid base indicator of natural plant pigment, to meet the demand of prior art.
Another object of the present invention is to provide the preparation method of above-mentioned acid base indicator.
Prunus cerasifera (
prunus cerasifera) be the defoliation small arbor of rose family Prunus, its true leaf sheet is aubergine in the whole season of growth.Containing a large amount of Cyanidins in Purple Leaf Plum, obvious to the change instruction of pH value of solution, when pH value of solution≤5.5, present stable shiny red, during pH value of solution >=6.5, then present yellow green, pH mutational range (5.5-6.5) is narrow, is only the pH of 1 unit, and color change obviously, be easy to judge, and stable performance.
Therefore, technical scheme of the present invention is as follows:
Based on an acid base indicator for natural plant pigment, it is characterized in that described acid base indicator is the natural colouring matter that take Purple Leaf Plum as Cyanidin that raw material extracts is principal ingredient.
Based on a preparation method for the acid base indicator of natural plant pigment, comprise the following steps: gather prunus cerasifera aubergine blade, running water is clean, and at-10 ~ 25 DEG C after drying, vacuum freeze drying 8-24h under 10-100Pa, is ground into powder; At lucifuge and 25 ~ 40 DEG C, extract 2-5 time by mass volume ratio 5 ~ 20% volume ratio 99:1 methanolic hydrochloric acid (99.0ml methyl alcohol+1.0ml volume fraction is the concentrated hydrochloric acid of 37%); Combining extraction liquid, preserve at lucifuge 0-4 DEG C, gained extract is acid base indicator.
Advantage of the present invention is mainly reflected in following several respects: the acid base indicator pH mutational range that (1) obtains is narrow, between 5.5-6.5; Sensitive to pH change instruction; (2) color change is distinct, is become the yellow green of alkali formula, be easy to judge terminal from the shiny red of acid; (3) produce or use procedure in raw materials used be natural plants or low toxicity industrial chemicals, product and production run environmental protection; (4) raw material sources are extensive, preparation technology is simple, are easy to large-scale production.
Embodiment
The present invention is described in detail below by specific embodiment.
embodiment 1:
20, prunus cerasifera aubergine blade is gathered in Chinese Marine University Yu Shan school district, Shinan District, Qingdao of Shandong province, running water is clean, at-10 DEG C after drying, vacuum freeze drying 24h under 100Pa, be ground into powder, at lucifuge and 25 DEG C, gained 2g powder 40ml methanolic hydrochloric acid extract 2 times (adopt 99.0ml methyl alcohol+1.0ml volume fraction be 37% concentrated hydrochloric acid formulated), combining extraction liquid, be positioned in brown glass drop bottle, preserve at 4 DEG C, namely gained extract can be used as acid base indicator.
After the hydrochloric acid of 0.1mol/L and the sodium bicarbonate solution of 0.1mol/L are mixed in the ratio of 1:5-5:1, and apply the pH value that pH meter measures mixed solution respectively, its serial mixed solution pH value range is between 1.5-8.5, then drip 2-3 respectively and drip prepared acid base indicator, solution ph lower than 5.5 time, present shiny red, and reduce shiny red enhancing with pH value; Solution ph higher than 6.5 time, present yellow green, show that prepared indicator pH mutational range is 5.5-6.5.
Accurately pipette the sodium bicarbonate solution that 25.00mL concentration is about 0.1mol/L and be placed in 250mL triangular pyramidal bottle, add 2-3 and drip prepared acid base indicator, solution presents yellowish green element, then titration is carried out with the hydrochloric acid solution of concentration 0.1050mol/L, in titration process when hydrochloric acid solution is reached for 24.50ml, sodium bicarbonate and hydrochloric acid neutralization reaction system sport shiny red by yellowish green element, and keep for 30 seconds colour-fast, show that titration reaches terminal.Calculating sodium bicarbonate solution concentration is 0.1029mol/L.
embodiment 2:
100, prunus cerasifera aubergine blade is gathered on both sides of the road in the East Sea, Qingdao of Shandong province, running water is clean, at-25 DEG C after drying, vacuum freeze drying 8h under 10Pa, is ground into powder, at lucifuge and 25 DEG C, gained powder 10g 50ml methanolic hydrochloric acid extract 5 times (adopt 99.0ml methyl alcohol+1.0ml volume fraction be 37% concentrated hydrochloric acid formulated), combining extraction liquid, preserve at lucifuge 0 DEG C, namely gained extract can be used as acid base indicator.
Accurately pipette the hydrochloric acid solution that 25.00mL concentration is about 0.1mol/L and be placed in 250mL triangular pyramidal bottle, add 2-3 and drip prepared acid base indicator, solution presents shiny red, then titration is carried out with the sodium carbonate liquor of concentration 0.0506mol/L, in titration process when hydrochloric acid solution is reached for 23.85ml, hydrochloric acid and sodium carbonate neutralization reaction system sport yellowish green element by shiny red, and keep for 30 seconds colour-fast, show that titration reaches terminal.Calculating concentration of hydrochloric acid solution is 0.09654mol/L.
Claims (2)
1., based on an acid base indicator for natural plant pigment, it is characterized in that described acid base indicator is take Purple Leaf Plum as the natural colouring matter that raw material extracts.
2. based on a preparation method for the acid base indicator of natural plant pigment, it is characterized in that comprising the following steps: gather prunus cerasifera aubergine blade, running water is clean and after drying, at-10 ~-25 DEG C, and vacuum freeze drying 8-24h under 10-100Pa; Be ground into powder, at lucifuge and 25-40 DEG C, extract 2-5 time by the methanolic hydrochloric acid of mass volume ratio 5 ~ 20% with volume ratio 99:1; Combining extraction liquid, preserve at lucifuge 0-4 DEG C, gained extract can be used as acid base indicator.
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| CN201510344141.XA CN104880461A (en) | 2015-06-22 | 2015-06-22 | Acid-base indicator based on natural plant pigment and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105784702A (en) * | 2016-05-23 | 2016-07-20 | 江苏耐雀生物工程技术有限公司 | Acid-base indicator and preparation method thereof |
| CN109738430A (en) * | 2019-03-02 | 2019-05-10 | 福建集成伞业有限公司 | Soda acid indicates function umbrella |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101162219A (en) * | 2006-10-13 | 2008-04-16 | 广东海洋大学 | Pigment from mangrove kenaelia candel ph indicator |
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2015
- 2015-06-22 CN CN201510344141.XA patent/CN104880461A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101162219A (en) * | 2006-10-13 | 2008-04-16 | 广东海洋大学 | Pigment from mangrove kenaelia candel ph indicator |
Non-Patent Citations (5)
| Title |
|---|
| M.E.SOLTAN* AND S.M.SIRRY: "Usefulness of Some Plant Flowers as Natural Acid-base Indicators", 《JOURNAL OF THE CHINESE CHEMICAL SOCIETY》 * |
| 史宝胜: "紫叶李叶色生理变化及影响因素研究", 《中国博士学位论文全文数据库 农业科技辑》 * |
| 张志: "紫叶李紫叶碧桃叶片光合特性及色素理化性质研究", 《中国优秀硕博士学位论文全文数据库（硕士） 农业科技辑》 * |
| 张莲姬等: "效果良好的天然指示剂——金达莱花色素", 《延边大学学报（自然科学版）》 * |
| 李玲: "五种红色叶植物叶片色素分析", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105784702A (en) * | 2016-05-23 | 2016-07-20 | 江苏耐雀生物工程技术有限公司 | Acid-base indicator and preparation method thereof |
| CN105784702B (en) * | 2016-05-23 | 2018-10-02 | 江苏耐雀生物工程技术有限公司 | A kind of acid-base indicator and preparation method thereof |
| CN109738430A (en) * | 2019-03-02 | 2019-05-10 | 福建集成伞业有限公司 | Soda acid indicates function umbrella |
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