CN104873212B - The coaxial imaging in vivo method and system of bimodulus - Google Patents

The coaxial imaging in vivo method and system of bimodulus Download PDF

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CN104873212B
CN104873212B CN201510165665.2A CN201510165665A CN104873212B CN 104873212 B CN104873212 B CN 104873212B CN 201510165665 A CN201510165665 A CN 201510165665A CN 104873212 B CN104873212 B CN 104873212B
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bimodulus
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CN104873212A (en
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黄国亮
汪汝亮
林雪
薛宁
白净
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Tsinghua University
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Abstract

The coaxial imaging in vivo method and system of bimodulus belongs to imaging in vivo detection technique and scientific instrument field.The system of the present invention includes X ray and optical signal double-mode imaging detector, colour filter switching device, optical imagery module, rotatable stage, x-ray source, more than one light source and motion controller, light source switch controller, signal acquisition digital processor and microprocessor；Microprocessor connection motion controller, light source switch controller and the signal acquisition digital processor；X ray and optical signal double-mode imaging detector include an opto-electronic conversion image device, an optical fiber cone, the XRF upconversion fiber panel and probe body of a printing opacity.Compared with other toy bimodulus in the world or multimode imaging in vivo detecting instrument, the present invention has the characteristics of bimodulus coaxial parallel real-time imaging, and without rotary registration when image co-registration is rebuild, and its is simple and compact for structure, and cost is cheap, is with a wide range of applications.

Description

The coaxial imaging in vivo method and system of bimodulus
Technical field
The invention belongs to imaging in vivo detection technique and scientific instrument field, more particularly to a kind of bimodulus is coaxial or coaxial The design of imaging in vivo technology and system architecture.
Background technology
Key link of the toy in the detection of body molecular image is medical system biological study, and by gene protein The scientific researches such as the vitro detection of molecule and cellular level are explored to the intermediate axle link beam in the application conversion of body clinical detection.Wherein, Multi-modality imaging system based on X ray, CT, PET and optical fluorescence molecular tomographic (FMT) is widely used to zoopery Research, existing multiple modalities are imaged and merged by Computer Image Processing, biotomy structure, life can be obtained The information of function of science and molecule or cellular level biological action etc..
FMT is merged the FMT/microCT double-mode imaging systems that establish with minitype CT (microCT) by Kepshire etc., is used Studied in the imaging experiment of toy, but the collection of FMT therein and microCT dual mode datas be not carry out simultaneously, it is necessary to Realize that the sequencing of two modal datas gathers by mobile animal beds.It is orthogonal that Schulz etc. establishes FMT/XCT bimodulus Small animal imaging system, by rotatable stage acquisition order fluorescence signal and x-ray projection signal, realize that full angle is freely empty Between projection data acquisitions, FMT systems therein and XCT systems are put the mutually orthogonal form of component space, angle, often gathered , it is necessary to which rotatable stage suspends after adjusting to specified location after FMT systems (or XCT systems) data, then gather an XCT System (or FMT systems) data, the FMT systems and XCT systems of object correspondence position are realized finally by Computer Image Processing The bimodulus image co-registration of collection.
At home, Tsing-Hua University and Institute of High Energy Physcis, Academia Sinica have developed bimodal FMT/PET petty actions cooperatively Thing imaging system, Institute of Automation Research of CAS develop bimodal FMT/CT small animal imaging systems, are using sky Between with angle segmentation scheme.Tumor bearing nude mice, breast cancer etc. under being treated with these systems in drug carry out imaging in vivo, can be with Situations such as observing the intake of tumour cell glucose, the synthesis of red fluorescent protein and the control of tumour simultaneously.
Domestic and international bimodulus or multimode imaging in vivo system architecture are made a general survey of, presently mainly using space partitioning scheme, is passed through Mobile or rotating object, using a variety of detectors different modalities are carried out with individually order and is imaged, by setting some characteristic points to match somebody with somebody Level is put, and then carries out image co-registration by computer software, realizes bimodulus or multimode imaging in vivo function.Such bimodulus is more Mould imaging in vivo system, the different view directions position of different detection patterns separations is not only due to, can not be accomplished to same target The real-time imaging in vivo of multimodal information of same view angle, and system architecture is complicated, and sufficiently bulky, cost is very high, expensive, on Ten million yuan/platform, thus the serious popularization and application for constraining imaging in vivo system.Nevertheless, detected in toy imaging in vivo Technical research and scientific instrument development aspect, integrate X ray, CT, PET, MRI and optical molecular imaging technology (OMI), carry out excellent Gesture is complementary, the information of the different aspects such as display structure and function simultaneously on piece image, gives full play to the high score of optical imagery Resolution characteristic and high-energy ray high-penetration depth obtain the ability of anatomic information, aid in OMI high sensitivity and acquisition point The ability of sub-information, it is still important development direction of the toy in body molecular imaging.
Toy imaging in vivo detection technique research is carried out to develop, it is necessary to which the key in face of three aspects is asked with scientific instrument Topic.One is system schema and stage apparatus structure, novel system architecture conceptual design can simplify with stage apparatus structure System architecture, volume is reduced, lowering apparatus cost, improves overall performance index.The second is detector, it be toy body into The core component of picture, expensive, hundreds of thousands-member/platform up to a million, and different image modes need to use different types of spy Device is surveyed, as optical imagery use cryogenic refrigeration ccd detector, x-ray imaging and CT use flat panel detector, and PET uses crystal The high energy ray detector of conversion, for MRI using magnetic coil etc., there is presently no see that a detector can compatibility two simultaneously The report of kind or more detection mode imaging, therefore, develop the imaging detection device of compatible two kinds or more patterns, multimode is existed Body imaging is significant.The key issue of 3rd aspect is that excitaton source can not be unified, and mainly having x-ray source at present, (X is penetrated Line image and CT), γ high-energy rays source (PET), permanent magnet and magnetic coil (MRI), ultraviolet-visible-infrared light supply (optics into Picture).
As can be seen here, the innovation of bimodulus or multimode imaging in vivo technology and system is carried out, simplied system structure, merges detection The use of device, the species and quantity of detector are reduced, reduces cost, the development and scientific instrument to imaging in vivo detection technique Popularization and application are very important.
The content of the invention
It is dexterous it is an object of the invention to provide a kind of structure, a detector is shared, without object movement or rotation, so that it may To realize the coaxial imaging in vivo method and system of bimodulus of bimodulus fusion autoregistration, optics and X ray can be realized using it The coaxial or coaxial parallel imaging in vivo of bimodulus.
The coaxial imaging in vivo method and system of bimodulus of the present invention, it is characterised in that described bimodulus coaxially body into Image space method shares a detector and carries out optics and the coaxial imaging in vivo of X ray bimodulus, without object movement or rotation, without setting Put feature registration point, it is possible to realize bimodulus information autoregistration；The coaxial imaging in vivo system of described bimodulus is penetrated comprising an X Line and optical signal double-mode imaging detector, a colour filter switching device, an optical imagery module, a rotatable stage, One x-ray source, more than one light source, a motion controller, a light source switch controller, a signal acquisition digitlization Processor and a microprocessor；Microprocessor connection motion controller, light source switch controller and the signal acquisition numeral Change processor.
A kind of coaxial imaging in vivo system of bimodulus, it is characterised in that：
Include X ray and optical signal double-mode imaging detector, colour filter switching device, optical imagery module, rotation load Thing platform, x-ray source, more than one light source and motion controller, light source switch controller, signal acquisition digital processor and micro- Processor；Microprocessor connection motion controller, light source switch controller and the signal acquisition digital processor；
X ray and optical signal double-mode imaging detector, rotatable stage and x-ray source coaxial placement, light source are positioned over rotation Turn around objective table；Colour filter switching device is set between rotatable stage and X ray and optical signal double-mode imaging detector With optical imagery module,
X ray and optical signal double-mode imaging detector include an opto-electronic conversion image device, and the X ray of a printing opacity is glimmering Light upconversion fiber panel and probe body.
Further, opto-electronic conversion image device is CCD or CMOS photoelectric conversion array devices.
Further, the XRF upconversion fiber panel of the printing opacity, is done by rare earth material doped-glass drawing optical fiber Into.
Further, the light source carries out transmission-type or side that is reflective or exciting fluorescence to testee on rotatable stage Formula illuminates.
Further, the rotatable stage includes a rotatable stage seat, a stay-warm case and an electric rotating machine.
Further, the optical imagery module be lens or binary optical elements or Fresnel Lenses or hololens or it Mixed structure.
Further, in addition to an optical fiber cone, the XRF upconversion fiber panel of printing opacity are close to optical fiber cone Large end face, optical fiber cone small end face are connected with the photosurface of opto-electronic conversion image device；
Or the XRF upconversion fiber panel of printing opacity is close to the small end face of optical fiber cone, optical fiber cone large end face It is connected with the photosurface of opto-electronic conversion image device.
Further, X ray and optical signal the double-mode imaging detector is not when having optical fiber cone, by the X ray of printing opacity Fluorescence upconversion fiber panel abuts directly on to be used on the photosurface of opto-electronic conversion image device.
Using the method for described system, testee is fixed on rotatable stage；
It is characterized in that：
Motion controller, light source switch controller and signal acquisition digital processor are controlled by microprocessor, simultaneously X-ray source and light source are opened, colour filter switching device is placed in neutral gear, adopted by X ray and optical signal double-mode imaging detector Collect signal, obtain X ray and the coaxial imaging in vivo of optical bimodulus in real time；Using different emission fluorescence labeling material and X-ray imaging material process testee, time-modulation is carried out to the opening of x-ray source and light source by microprocessor, The time-modulation of colour filter switching device and light source activation fluorescent state is synchronous, using the fluorescence labeling material of different emission The cell or biomolecule of material mark testee, X ray and fluorescence are obtained respectively in body image, after image procossing, is obtained Obtain the coaxial imaging in vivo of bimodulus of X ray and cell or biomolecule fluorescent characteristics；
Or control motion controller to rotate objective table by microprocessor, drive testee to rotate, use X Ray and optical signal double-mode imaging detector synchronous acquisition signal, X ray and the optical bimodulus for obtaining testee in real time are same Axle is in body local tomography or fault imaging；The topography that will be rotated a circle, three-dimensional reconstruction is carried out on the microprocessor, obtain quilt 360 ° of coaxial panoramas of object bimodulus are surveyed in body 3-D view, optical molecular tomographic and X ray computer fault imaging；
Or motion controller, light source switch controller and signal acquisition digital processor are controlled by microprocessor, Order opens x-ray source and light source, and makes the time-modulation of colour filter switching device and light source activation fluorescent state synchronous, in X X ray or the single modality images of optics or fluorescence are gathered on ray and optical signal double-mode imaging detector, then by micro- place Reason device is handled these single modality images, then carries out image co-registration.
The coaxial imaging in vivo method and system of bimodulus of the present invention, it is characterised in that the X ray and optical signal are double Mould imaging detector is glimmering by an opto-electronic conversion image device (CCD, CMOS etc.), an optical fiber cone, the X ray of a printing opacity Light upconversion fiber panel and probe body composition；The small end face of the optical fiber cone is used with opto-electronic conversion image device photosurface Optics ultraviolet glue is coupled, and large end face couples again by optics ultraviolet glue with the XRF upconversion fiber panel of printing opacity； The XRF upconversion fiber panel of the printing opacity, it can use the molten forming method processed of flicker glass of silicate activated by terbium will be dilute Soil material (such as Tb3+) doped silicate glasses (or heavy metallic oxide scintillation glass, aluminosilicate oxygen fluorine glass, borosilicate Other glass such as glass) in be made glass bar (Zhao Jianjun etc., light journal, V6, p655-660,2004；Zu Chengkui etc., silicon Hydrochlorate journal, V3, p283-286,2004；Jiang Daiwu etc., light journal, V3, p363-367, and 2009；Care for male etc., Chinese invention Patent, patent No. ZL200610118620.0；Guan Zhou states etc., Chinese invention patent, application number 201110421904.8), then Optical fiber fabrication is drawn into fibre faceplate, the function of fluorescence is not only converted into X ray, while have well to visible ray Transmission performance.
The coaxial imaging in vivo method and system of bimodulus of the present invention, it is characterised in that the X ray and optical signal are double Mould imaging detector, rotatable stage and x-ray source coaxial placement, light source are positioned over around objective table；The light source can be Monochromatic light, quasi-monochromatic light, white light, infrared ray, ultraviolet etc., transmission or reflection can be carried out to testee on rotatable stage Or excite fluorescent illumination.
The coaxial imaging in vivo method and system of bimodulus of the present invention, it is characterised in that the rotary carrier platform can be held Testee is carried, is rotated around object central shaft, can be in 360 degree of scope panoramic imageries, or in any angular range office Portion is imaged, and realizes the three-dimensional reconstruction of X ray computer fault imaging (XCT) and optical molecular tomographic (OMT).
The present invention has advantages below due to taking the above to design：
1st, the coaxial imaging in vivo method and system of bimodulus of the invention, a detector is shared, without object movement or rotation Turn, without setting feature registration point, it is possible to realize the imaging in vivo of the coaxial autoregistration of bimodulus, optical molecular can be carried out The bimodulus of fault image (OMT) and X ray computer fault imaging (XCT) detects, and displays one's respective advantages, and obtains one completely Toy obtains the bimodulus information of toy structure and molecular function aspect in body molecular image image.
2nd, a kind of X ray and the coaxial imaging detector of optical signal bimodulus information are provided with the present invention, it is possible to achieve optics The coaxial imaging in vivo with X ray bimodulus, also, the bimodulus information obtained in same direction can reach near real-time imaging It is horizontal.Traditional bimodulus or multimode imaging in vivo system architecture, optical detector and X-ray detector use space partitioning scheme Arrangement, by mobile or rotating object, using a variety of detectors different modalities are carried out with individually order and is imaged, then feature is set Registration point, image co-registration is carried out by computer software, realizes bimodulus or multimode imaging in vivo function, the detector of different mode It is discrete, and same imaging shaft can not be accomplished.Therefore, traditional bimodulus or multimode imaging in vivo system can not possibly realize same side To the real time imagery of bimodulus information, that is to say, that same direction obtain bimodulus information forever can not be synchronous, keep one compared with Big time interval.
3rd, the present invention in printing opacity XRF upconversion fiber panel, be one kind by rare earth material (such as Tb3+) doping silicic acid The fibre faceplate that salt glass (or other glass) drawing optical fiber is made, not only there is the function that X ray is converted into fluorescence, simultaneously There is good transmission performance to visible ray.Traditional bimodulus or multimode imaging in vivo system architecture, are by XRF Transition material plated film or the surface for being grown in fibre faceplate, or directly use X-ray luminescence conversion crystal strip array (such as BGO Crystalline material) X ray is converted into fluorescence, these methods have very strong attenuation to visible ray, almost impermeable visible Light, therefore, it is impossible to realize the directly coaxial image checking of bimodulus information of X ray and optical signal.
Brief description of the drawings
Fig. 1 is the coaxial imaging in vivo system structure diagram of bimodulus of the present invention；
Fig. 2 is the X ray and the coaxial imaging detector structural diagrams of optical signal bimodulus of the present invention.
Embodiment
Come to carry out the present invention detailed description below in conjunction with accompanying drawing.It should be appreciated, however, that accompanying drawing has been provided only more Understand the present invention well, they should not be interpreted as limitation of the present invention.
To achieve the above object, the invention provides a kind of coaxial imaging in vivo system of bimodulus, topology example is embodied As shown in figure 1, including an X ray and optical signal double-mode imaging detector (10), a colour filter switching device (15), one Optical imagery module (16), a rotatable stage, an x-ray source (23), more than one light source, a motion controller (32), a light source switch controller (33), a signal acquisition digital processor (31) and a microprocessor (30).Its In, microprocessor (30) connection motion controller (32), light source switch controller (33) and signal acquisition digital processor (31)；X ray and optical signal double-mode imaging detector (10), colour filter switching device (15), optical imagery module (16), rotation At the center of objective table and x-ray source (23) on the same line, can carry out X ray and optical signal bimodulus coaxially body into Picture.
A kind of specific implementation topology example of X ray and optical signal double-mode imaging detector is as shown in Fig. 2 include a spy Device shell (11), a CCD or cmos imaging device (12), an optical fiber cone (13) are surveyed, the XRF of a printing opacity turns Change fibre faceplate (14)；Wherein, optical fiber cone (12) small end face passes through optics ultraviolet glue and CCD or cmos imaging device (12) Photosurface is of coupled connections, and the XRF upconversion fiber panel (14) of printing opacity is close to optical fiber light by the coupling of optics ultraviolet glue Bore the large end face of (13).
Light source can be that laser or LED or other polychrome lamp sources, light source are separately mounted to the week of the rotatable stage Enclose, can be used according to system needs to reflect the testee (00) on rotatable stage or transmitted or excite fluorescence to shine It is bright.
Rotatable stage is by a rotatable stage seat (42), a stay-warm case (41), electric rotating machine (43) composition. Wherein, stay-warm case (41) has heating and heat preserving function, and top can be passed through anesthetic gases, and testee (00) is fixed on stay-warm case (41) center；Electric rotating machine can be direct current generator, or stepper motor, or servomotor, or manpower drive control structure.
Optical imagery module (16) is made up of thin lens or binary optical elements or Fresnel Lenses or hololens etc., right X ray has good transmissivity, can carry out upright optical imagery to testee (00), and with the X of testee (00) The size of ray projection imaging is consistent, and optical imagery and x-ray projection imaging are coaxial, are changed in the XRF of printing opacity Automatic alignment overlaps on fibre faceplate (14).
Based on system shown in Figure 1, the coaxial basic implementation steps of imaging in vivo method of bimodulus of the invention are as follows：
1st, stay-warm case (41) center being fixed on testee (00) (such as toy) on rotatable stage, by external Anesthesia machine be passed through anesthetic gases at the top of the stay-warm case (41), stay-warm case (41) is heated and tieed up using heat radiation or hot-air flowing Hold organism normal body temperature (such as 30 DEG C or so).
2nd, motion controller (32), light source switch controller (33) and signal acquisition numeral are controlled by microprocessor (30) Change processor, while open x-ray source (23) and light source, colour filter switching device (15) is placed in neutral gear, passes through X ray and light Signal double-mode imaging detector (10) gathers signal, can obtain X ray and the coaxial imaging in vivo of optical bimodulus in real time；Using The fluorescence labeling material and x-ray imaging material process testee (00) of different emission, in X ray and optical signal bimodulus Colored CCD or cmos imaging device (12) are used in imaging detector (10), X ray and light with color characteristic can be obtained Bimodulus is coaxially in body image；Time tune is carried out to the opening of x-ray source (23) and light source by microprocessor (30) System, the time-modulation of colour filter switching device (15) and light source activation fluorescent state is synchronous, using the glimmering of different emission The cell or biomolecule of signal material marking testee (00), X ray and fluorescence can be obtained respectively in body image, lead to After crossing pseudo-colours or other image procossings, outstanding behaviours X ray and cell or the bimodulus of biomolecule fluorescent characteristics can be obtained Coaxial imaging in vivo.
3rd, control motion controller (32) to rotate objective table by microprocessor (30), drive testee (00) to do and revolve Transhipment is dynamic, with X ray and optical signal double-mode imaging detector (10) synchronous acquisition signal, can obtain testee (00) in real time X ray and optical bimodulus coaxially in body local tomography or fault imaging；The topography that will be rotated a circle, in microprocessor (30) three-dimensional reconstruction is carried out on, 360 ° of coaxial panoramas of testee (00) bimodulus can be obtained in body 3-D view, optics point Sub- fault imaging (OMT) and X ray computer fault imaging (XCT).
4th, motion controller (32), light source switch controller (33) and signal acquisition numeral are controlled by microprocessor (30) Change processor, order opens x-ray source (23) and light source, and makes colour filter switching device (15) and light source activation fluorescent state Time-modulation is synchronous, and the list of X ray or optics or fluorescence can be gathered on X ray and optical signal double-mode imaging detector (10) One modality images, the surface topology image of X ray digital projection image (DR), testee (00) such as testee (00), The internal histocyte of testee (00) or biomolecule in body fluoroscopic image etc., avoid interacting between each mode, Then microprocessor (30) is handled these single modality images, then carries out image co-registration, and it is special can to obtain some The bimodulus of different enhancing effect is in body image.
Label 21 and 22 represents the first light source and secondary light source respectively, but is not limited to two light sources.
For the present invention by design above, the specific performance index that it can reach is as follows：
<1>System can realize the coaxial imaging in vivo of bimodulus, obtain bimodulus in real time in body information, avoid in conventional method Different mode image needs feature based Point matching to carry out complicated calculations that image co-registration brings and asynchronous；
<2>System can realize 0.1 degree of fine scanning image checking of 360 degree of panoramic ranges；
<3>System optics detection resolution 10-5000Lp/mm, 0-500mm of testee physical dimension；
<4>System X-ray detection resolution ratio reaches 10-5000Lp/mm, 0-500mm of testee physical dimension；
<5>Thermostatic control precision >=0.5 DEG C of system, 0-50 DEG C of variable temperatures scope.
The various embodiments described above are merely to illustrate the present invention, wherein the structure of each part, connected mode and manufacture craft etc. are all It can be varied from, every equivalents carried out on the basis of technical solution of the present invention and improvement, should not exclude Outside protection scope of the present invention.

Claims (9)

  1. A kind of 1. coaxial imaging in vivo system of bimodulus, it is characterised in that：
    Include X ray and optical signal double-mode imaging detector, colour filter switching device, optical imagery module, rotatable stage, X-ray source, more than one light source, motion controller, light source switch controller, signal acquisition digital processor and microprocessor Device；Microprocessor connection motion controller, light source switch controller and the signal acquisition digital processor；
    X ray and optical signal double-mode imaging detector, rotatable stage and x-ray source coaxial placement, light source are positioned over rotation and carried Around thing platform；Colour filter switching device and light are set between rotatable stage and X ray and optical signal double-mode imaging detector Learn image-forming module,
    X ray and optical signal double-mode imaging detector include an opto-electronic conversion image device, and the XRF of a printing opacity turns Fibre faceplate and probe body are changed,
    X-ray source and light source are opened simultaneously, signal is gathered by X ray and optical signal double-mode imaging detector, X is obtained in real time and penetrates Line and optical bimodulus are coaxially in body image；
    Control motion controller to rotate objective table by microprocessor, drive testee to rotate, with X ray and light Signal double-mode imaging detector synchronous acquisition signal, the X ray of acquisition testee and optical bimodulus are coaxially in body office in real time Portion's image；X-ray source and light source are opened simultaneously, colour filter switching device is placed in neutral gear, changes 0.1 degree of rotation one according to each Week obtains topography, carries out three-dimensional reconstruction on the microprocessor, obtains 360 ° of coaxial panoramas of testee bimodulus in body three Tie up image；Motion controller, light source switch controller and signal acquisition digital processor are controlled by microprocessor, sequentially opened X-ray source and light source are opened, and makes the time-modulation of colour filter switching device and light source activation fluorescent state synchronous, according to changing every time Become 0.1 degree of rotate a circle acquisition X ray or optics topography, carry out three-dimensional reconstruction on the microprocessor, obtain testee X ray computer faultage image or optical molecular faultage image.
  2. A kind of 2. coaxial imaging in vivo system of bimodulus according to claim 1, it is characterised in that：
    Opto-electronic conversion image device is CCD or CMOS photoelectric conversion array devices.
  3. A kind of 3. coaxial imaging in vivo system of bimodulus as claimed in claim 1, it is characterised in that：
    The XRF upconversion fiber panel of the printing opacity, is made by rare earth material doped-glass drawing optical fiber.
  4. A kind of 4. coaxial imaging in vivo system of bimodulus as claimed in claim 1, it is characterised in that：
    The light source carries out transmission-type to the testee on rotatable stage or reflective or excitation fiuorescent type illuminates.
  5. A kind of 5. coaxial imaging in vivo system of bimodulus as claimed in claim 1, it is characterised in that：The rotatable stage includes One rotatable stage seat, a stay-warm case and an electric rotating machine.
  6. A kind of 6. coaxial imaging in vivo system of bimodulus as claimed in claim 1, it is characterised in that：
    The optical imagery module is lens arrangement.
  7. A kind of 7. coaxial imaging in vivo system of bimodulus as claimed in claim 1, it is characterised in that：
    Also include an optical fiber cone, the XRF upconversion fiber panel of printing opacity is close to the large end face of optical fiber cone, optical fiber Light cone small end face is connected with the photosurface of opto-electronic conversion image device；
    Or the XRF upconversion fiber panel of printing opacity is close to the small end face of optical fiber cone, optical fiber cone large end face and light The photosurface connection of electric conversion imaging device.
  8. A kind of 8. coaxial imaging in vivo system of bimodulus as claimed in claim 1, it is characterised in that：
    When not having optical fiber cone, X ray and optical signal the double-mode imaging detector is by the XRF upconversion fiber of printing opacity Panel abuts directly on to be used on the photosurface of opto-electronic conversion image device.
  9. 9. the method for system of the application as described in claim 1-8 any one, testee is fixed on rotatable stage；
    It is characterized in that：
    Motion controller, light source switch controller and signal acquisition digital processor are controlled by microprocessor, while open X Radiographic source and light source, colour filter switching device is placed in neutral gear, passes through X ray and optical signal double-mode imaging detector collection letter Number, X ray and optical bimodulus are obtained in real time coaxially in body image；Penetrated using the fluorescence labeling material and X of different emission Line radiography material handles testee, carries out time-modulation to the opening of x-ray source and light source by microprocessor, will filter The time-modulation of color chips switching device and light source activation fluorescent state is synchronous, using the fluorescence labeling material mark of different emission Remember the cell or biomolecule of testee, obtain X ray and fluorescence respectively in body image, after image procossing, obtain X and penetrate Line and cell or the bimodulus of biomolecule fluorescent characteristics are coaxially in body image；
    Or control motion controller to rotate objective table by microprocessor, drive testee to rotate, use X ray With optical signal double-mode imaging detector synchronous acquisition signal, obtain the X ray of testee in real time and optical bimodulus coaxially exists Body topography；X-ray source and light source are opened simultaneously, colour filter switching device is placed in neutral gear, changes 0.1 degree of rotation according to each Circle and obtain topography, carry out three-dimensional reconstruction on the microprocessor, obtain 360 ° of coaxial panoramas of testee bimodulus and exist Body 3-D view；Motion controller, light source switch controller and signal acquisition digital processor are controlled by microprocessor, it is suitable Sequence opens x-ray source and light source, and makes the time-modulation of colour filter switching device and light source activation fluorescent state synchronous, according to every It is secondary to change 0.1 degree of rotate a circle acquisition X ray or optics topography, three-dimensional reconstruction is carried out on the microprocessor, is tested The X ray computer faultage image or optical molecular faultage image of object；
    Or motion controller, light source switch controller and signal acquisition digital processor, order are controlled by microprocessor X-ray source and light source are opened, and makes the time-modulation of colour filter switching device and light source activation fluorescent state synchronous, in X ray On optical signal double-mode imaging detector, you can be arranged on no colour filter in unlatching x-ray source and colour filter switching device Gather X ray single modality images during straight-through neutral position state, or open light source and colour filter switching device is arranged on and do not had Optics single modality images are gathered during the straight-through neutral position state of colour filter, or are opening light source and the setting of colour filter switching device The single modality images of fluorescence are gathered when there is the range state of colour filter, then microprocessor is to these single modal graphs As being handled, then carry out image co-registration.
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