CN104861051B - Plant development associated protein AtUBP15 and its encoding gene and application - Google Patents
Plant development associated protein AtUBP15 and its encoding gene and application Download PDFInfo
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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Abstract
The invention discloses a kind of plant development associated protein AtUBP15 and its encoding gene and application.Protein provided by the invention, AtUBP15 albumen is named as, is as follows（a）Or（b）Or（c）：（a）The protein being made up of the amino acid sequence shown in sequence in sequence table 1；（b）The protein being made up of the amino acid sequence shown in sequence in sequence table 2；（c）By substitution of the amino acid sequence of sequence 1 or sequence 2 by one or several amino acid residues and/or missing and/or addition and the protein as derived from sequence 1 or sequence 2 related to development of plants.The present invention also protects a kind of method for cultivating genetically modified plants, is that in the AtUBP15 channel genes purpose plant, will obtain genetically modified plants of the development better than the purpose plant.The present invention has substantial worth for plant breeding.
Description
Technical field
The present invention relates to a kind of plant development associated protein AtUBP15 and its encoding gene and application.
Background technology
Grain is the primary condition of human survival, as population quickly increases, cultivated area is persistently reduced, improves unit plane
Store up grain food yield naturally just into people solve food problem main path.
China is a country for very lacking soil, although area is not small, most of is unbroken mountains high mountain, height
Former desert, the Plain of livable preferably agriculture only accounts for the 12% of area, therefore raising per mu yield has king-sized importance to China.
Recently due to the application of bioengineering, improve per mu yield and show unprecedented huge chance.
Size of plant seed is highly important for crop yield.
The content of the invention
It is an object of the invention to provide a kind of plant development associated protein AtUBP15 and its encoding gene and application.
Protein provided by the invention, come from Columbia ecotype arabidopsis, be named as AtUBP15 albumen, be as
Under（a）Or（b）Or（c）：
（a）The protein being made up of the amino acid sequence shown in sequence in sequence table 1；
（b）The protein being made up of the amino acid sequence shown in sequence in sequence table 2；
（c）By substitution of the amino acid sequence of sequence 1 or sequence 2 by one or several amino acid residues and/or missing
And/or addition and the protein as derived from sequence 1 or sequence 2 related to development of plants.
In order that（a）Or（b）In protein be easy to purify, can in as sequence table sequence 1 or the amino shown in sequence 2
The amino terminal or the upper label as shown in table 1 of carboxyl terminal connection of the protein of acid sequence composition.
The sequence of the label of table 1
| Label | Residue | Sequence |
| Poly-Arg | 5-6（Usually 5） | RRRRR |
| Poly-His | 2-10（Usually 6） | HHHHHH |
| FLAG | 8 | DYKDDDDK |
| Strep-tag II | 8 | WSHPQFEK |
| c-myc | 10 | EQKLISEEDL |
It is above-mentioned（c）In protein can be artificial synthesized, also can first synthesize its encoding gene, then carry out biological expression and obtain.
It is above-mentioned（c）In the encoding gene of protein can be by by the DNA sequence dna shown in sequence in sequence table 3, sequence 4 or sequence 5
The codon of one or several amino acid residues is lacked, and/or carries out the missense mutation of one or several base-pairs, and/or
The coded sequence that its 5 ' end and/or 3 ' ends connect the label shown in table 1 obtains.
Encode the gene of the AtUBP15 albumen（AtUBP15 genes）Fall within protection scope of the present invention.
The AtUBP15 genes are as follows（1）Or（2）Or（3）Or（4）DNA molecular：
（1）DNA molecular shown in the sequence 3 of sequence table；
（2）DNA molecular of the code area as shown in the sequence 4 of sequence table or the sequence 5 of sequence table；
（3）Under strict conditions with（1）Or（2）The DNA sequence dna hybridization of restriction and the DNA of coded plant development associated protein
Molecule；
（4）With（1）Or（2）The DNA sequence dna of restriction at least has more than 90% homogeneity and coded plant development associated protein
DNA molecular.
Above-mentioned stringent condition can be as follows：50 DEG C, in 7% lauryl sodium sulfate（SDS）、0.5M NaPO4And 1mM
Hybridize in EDTA mixed solution, rinsed in 50 DEG C, 2 × SSC, 0.1%SDS；Can also be：50 DEG C, in 7%SDS, 0.5M
NaPO4Hybridize with 1mM EDTA mixed solution, rinsed in 50 DEG C, 1 × SSC, 0.1%SDS；Can also be：50 DEG C, 7%
SDS、0.5M NaPO4Hybridize with 1mM EDTA mixed solution, rinsed in 50 DEG C, 0.5 × SSC, 0.1%SDS；May be used also
For：50 DEG C, in 7%SDS, 0.5M NaPO4Hybridize with 1mM EDTA mixed solution, at 50 DEG C, 0.1 × SSC, 0.1%SDS
Middle rinsing；Can also be：50 DEG C, in 7%SDS, 0.5M NaPO4Hybridize with 1mM EDTA mixed solution, at 65 DEG C, 0.1 ×
Rinsed in SSC, 0.1%SDS；Or：In 6 × SSC, 0.5%SDS solution, hybridize under 65oC, then with 2 × SSC,
0.1%SDS and 1 × SSC, 0.1%SDS respectively wash film once.
Above-mentioned " homogeneity " refers to the sequence similarity with native sequence nucleic acid." homogeneity " can with the naked eye or computer is soft
Part is evaluated.Using computer software, the homogeneity between two or more sequences can use percentage（%）Represent, it can
With for evaluating the homogeneity between correlated series.
Expression cassette, recombinant vector, transgenic cell line or recombinant bacterium containing the AtUBP15 genes belong to the present invention
Protection domain.
The recombinant expression carrier of the gene can be contained with existing expression vector establishment.The expression vector can also include
3 ' end untranslated regions of foreign gene, i.e., process or gene expression comprising polyadenylation signals and any other participations mRNA
DNA fragmentation.The bootable polyadenylic acid of polyadenylation signals is added to 3 ' ends of mRNA precursor.Using described gene constructed heavy
During group expression vector, any enhanced promoter or constitutive promoter can be added before its transcription initiation nucleotides, it
Can be used alone or be used in combination with other promoters；In addition, when using the gene constructed recombinant expression carrier of the present invention,
Enhancer, including translational enhancer or transcriptional enhancer also can be used, but must be identical with the reading frame of coded sequence, to ensure
The correct translation of whole sequence.The source of the translation control signal and initiation codon is extensive, can be it is natural,
It can be synthesis.Translation initiation region can come from transcription initiation region or structural gene.For the ease of being identified and being sieved
Choosing, can be processed to the recombinant expression carrier, and the enzyme of color change or the base of luminophor can be produced by such as adding coding
Cause, resistant antibiotic marker or anti-chemical reagent marker gene etc..
The recombinant vector concretely following recombinant plasmid：AtUBP15 genes insertion plasmid pMDC99 is obtained
Recombinant plasmid.
The present invention also protects a kind of method for cultivating genetically modified plants, is by the AtUBP15 channel genes purpose plant
In, obtain the genetically modified plants that developmental level is better than the purpose plant.The AtUBP15 genes can pass through the recombinant vector
Import the purpose plant.The AtUBP15 genes specifically can import the purpose plant by the recombinant plasmid.The side
In method, the recombinant vector can be by using Ti-plasmids, Ri plasmids, plant viral vector, directly delivered DNA, microinjection, electricity
Conventional biology methods conversion plant cell or the tissue such as lead, be agriculture bacillus mediated, and the plant tissue of conversion is cultivated into plant.
The purpose plant is monocotyledon or dicotyledon.The dicotyledon concretely arabidopsis, such as Colombia
Arabidopsis thaliana ecotype." developmental level is excellent " can specifically be presented as that seed is big and/or seed weight is big and/or it is big to spend and/or
Blade is big and/or cell propagation is fast.
In the above method, AtUBP15 genes can be modified first as follows, then import purpose plant, to reach more preferable table
Up to effect：
1. modified and optimized according to being actually needed, so that gene efficient expression；For example, can be according to recipient plant institute partially
The codon of love, the codon optimization of DNA level is carried out while amino acid sequence is kept to meet plant-preference；Optimization
During, it is desirable that certain G/C content is kept in the DNA after optimization, to be best implemented with the high level expression of gene, its
Middle G/C content can be 35%, 45%, 50% or more than 60%；
2. the nucleotide sequence of the neighbouring initial methionine of modification, so that translation effectively starting；For example, using in plant
Known effective sequence is modified；
3. it is connected with the promoter of various plants expression, in favor of its expression in plant；The promoter may include
Composing type, induction type, sequential regulation, growth adjustment, Chemical Regulation, tissue are preferably and tissue-specific promoter；Promoter
Selection will need and change with expression time and space, and also depend on target kind；Such as the specificity of tissue or seed
Promoter is expressed, acceptor as needed is depending on what period of development；Although demonstrate many from dicotyledon
Promoter can act in monocotyledon, and vice versa, but it is desirable to select dicot promoters are used for
Expression in dicotyledon, the expression that monocotyledonous promoter is used in monocotyledon；
4. being connected with suitable transcription terminator, the expression efficiency of gene can also be improved；Such as from CaMV's
Tml, from rbcS E9；Any known available terminator to be worked in plant can enter with gene of the present invention
Row connection；
5. enhancer sequence is introduced, such as intron sequences（Such as from Adhl and bronzel）And viral leader sequence
（Such as from TMV, MCMV and AMV）.
The present invention also protects the AtUBP15 albumen, the AtUBP15 genes or the recombinant vector cultivating development water
Application in flat excellent genetically modified plants.The plant that sets out of the genetically modified plants is monocotyledon or dicotyledon.Institute
Dicotyledon concretely arabidopsis is stated, such as Columbia ecotype arabidopsis." developmental level is excellent " is embodied as
Seed is big and/or seed weight is big and/or spends big and/or blade big and/or cell propagation is fast.
The present invention has substantial worth for plant breeding.
Brief description of the drawings
Fig. 1 is the relative expression quantity of AtUBP15 genes
Fig. 2 compares for plant phenotype；Scale is respectively 0.5mm, 5cm and 1mm from top to bottom；It is horizontal that * represents P ＜ 0.01
Significant difference.
Fig. 3 is the apparent surface's projected area and relative weight of single seed.
Fig. 4 is the 5th true leaf of plant（Maturity period）Blade area and blade cell area.
Fig. 5 is second on the stem of plant（Maturity period）To the 5th flower（Maturity period）Single flower average petal
Area, average petal length, average petal width and average petal cell area.
Embodiment
Following embodiment facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method, it is conventional method unless otherwise specified.Test material used in following embodiments, it is certainly unless otherwise specified
What routine biochemistry reagent shop was commercially available.Quantitative test in following examples, it is respectively provided with and repeats to test three times, as a result make even
Average.
Entry vector pDONR207：Purchased from Invitrogen companies, article No. 12213-013.
Plasmid pMDC99：Bibliography：Curtis MD,Grossniklaus U(2003)A gateway cloning
vector set for high-throughput functional analysis of genes in planta.Plant
Physiol.133:462-469。
Agrobacterium tumefaciems GV3101：Bibliography：Li, Y., Zheng, L., Corke, F., Smith, C., and Bevan,
M.W.(2008)；Control of final seed and organ size by the DA1gene family in
Arabidopsis thaliana.Genes Dev22,1331-1336.
Columbia ecotype arabidopsis（Col-0）：ABRC（Arabidopsis Biological Resource
Center）, seed numbering CS28166.
T1It is T for the plant that seed grows up to1For plant.T2It is T for the plant that seed grows up to2For plant.T3Grow up to for seed
Plant be T3For plant.
Inventor has found a protein from Columbia ecotype arabidopsis, as shown in the sequence 1 of sequence table or such as
Shown in the sequence 2 of sequence table（Two kinds of shear patterns）, it is named as AtUBP15 albumen.The gene of AtUBP15 albumen will be encoded
AtUBP15 genes are named as, its full-length genome is as shown in the sequence 3 of sequence table, the institute of sequence 4 of ORFs such as sequence table
Show or as shown in the sequence 5 of sequence table（Two kinds of shear patterns）.
Embodiment 1, the transfer-gen plant excellent by importing AtUBP15 genes cultivation developmental level
First, the preparation of recombinant plasmid
1st, the genomic DNA of Columbia ecotype arabidopsis is extracted.
2nd, the genomic DNA extracted using step 1 is entered as template using the AtUBP15-F and AtUBP15-R primer pairs formed
Performing PCR expands, and obtains pcr amplification product.
AtUBP15-F:5'-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCCTCAAATCCACAAAAAATCCGA；
AtUBP15-R:5'-GGGGACCACTTTGTACAAGAAAGCTGGGTCCTAAAGCTCTGCTTAGTGAACC。
Underscore mark attB1 sites in AtUBP15-F.Underscore mark attB2 sites in AtUBP15-R.
BP reactions occur for the pcr amplification product and entry vector pDONR207 that the 3, step 2 obtains, and obtain containing ordered list
The recombinant plasmid of DNA molecular shown in sequence 2（It is named as recombinant plasmid pDNOR207-AtUBP15）.
4th, LR reactions occur for recombinant plasmid pDNOR207-AtUBP15 and plasmid pMDC99, obtain the sequence containing ordered list
The recombinant plasmid of DNA molecular shown in 2（It is named as recombinant plasmid pMDC99-AtUBP15）.
2nd, the acquisition of genetically modified plants
1st, recombinant plasmid pMDC99-AtUBP15 is imported into Agrobacterium tumefaciems GV3101, obtains recombinational agrobacterium.
2nd, the seed of Columbia ecotype arabidopsis is taken, 1/2MS solid mediums, 4 DEG C of vernalization 3 are laid in after sterilization
My god, in normal condition（22 DEG C/18 DEG C, 16h illumination/8h is dark, 75% humidity）Lower culture 8 days, is then transplanted to soil property culture medium
（Turfy soil：Vermiculite=1：2；Volume ratio）And cultivated 3 weeks in greenhouse, seedling starts bolting and bloomed.
3rd, with conversion buffer solution（Sucrose containing 5g/100ml, 0.05g/100ml MES, 0.03% volume ratio Silwet
L-77 1/2MS fluid nutrient mediums, pH5.7）The recombinational agrobacterium that step 1 obtains is resuspended, obtains OD600nm=0.3-0.5 bacterium is hanged
Liquid.
4th, the plant for taking step 2 to obtain, the bacteria suspension obtained with suction pipe aspiration step 3, careful drops on titbit, repeats
Drop 3-5 times, then covers plant with plastic sheeting and carries out moisture-heat preservation, removes film after 2 days by plant normal growth, harvest kind
Son, as T1For seed.
5th, by T1For seed sowing in the 1/2MS solid mediums of the hygromycin containing 50mg/L, normal culture（Can normal growth
Plant be resistant plant, the plant that can only grow the just not regrowth of tiny cotyledon is sensitive plant）, collect resistant plant
Seed, as T2For seed.
6th, by T2For seed sowing in the 1/2MS solid mediums of the hygromycin containing 50mg/L, normal culture（Can normal growth
Plant be resistant plant, the plant that can only grow the just not regrowth of tiny cotyledon is sensitive plant）, collect resistant plant
Seed, as T3For seed.
7th, by the T of sampling3For seed sowing in the 1/2MS solid mediums of the hygromycin containing 50mg/L, normal culture（Can be just
The plant being frequently grown is resistant plant, and the plant that can only grow the just not regrowth of tiny cotyledon is sensitive plant）.
For a certain T2For plant, if the T of its sampling Detection3It is positive plant for plant, the T2It is for plant
Homozygous transfer-gen plant, the plant and its self progeny are a homozygous transgenic line.
3rd, the acquisition of empty carrier plant is turned
Replace recombinant plasmid pMDC99-AtUBP15 to carry out step 2 with plasmid pMDC99, obtain turning empty carrier plant.
4th, Molecular Identification
Take Columbia ecotype arabidopsis, 3 homozygous transgenic lines（OE1 strains, OE2 strains and OE3 strains）
T3 for plant, extraction total serum IgE and reverse transcription be cDNA, the primer pair identification formed using qUBP15-F and qUBP15-R
AtUBP15 genes, the primer pair formed using ACTIN7F and ACTIN7R identify ACTIN7 genes（Reference gene）, calculate
The relative expression quantity of AtUBP15 genes, is as a result shown in Fig. 1（The average value of 3 repetition plant）.
qUBP15-F：5’-GGAGACGTTCCTCCGCTTTATATGC-3’；
qUBP15-R：5’-TCCTCTTTGAGGACGTGGATACGAT-3’.
ACTIN7F：5’-ATCCTTCCTGATATCGAC-3’；
ACTIN7R：5’-GAGAAGATGACTCAGATC-3’.
5th, phenotypic evaluation
Take the seed of Columbia ecotype arabidopsis（30）, 3 homozygous transgenic lines（OE1 strains, OE2 strains
System and OE3 strains）T3For seed（Each strain 30）, turn the T of empty carrier plant3For seed（30）, carry out respectively as follows
Operation：Seed is taken, is laid in 1/2MS solid mediums after sterilization, 4 DEG C of vernalization 3 days, then in normal condition（22 DEG C/18 DEG C,
16h illumination/8h is dark, 75% humidity）Lower culture 8 days, is then transplanted to soil property culture medium（Turfy soil：Vermiculite=1：2；Volume ratio）
And cultivated in greenhouse, harvest the early stage seed of natural maturity on plant stem（The usually seed of the 2nd to the 10th silique）.
The photo of the seed of part harvesting is shown in Fig. 2A.Kind of the seed of transfer-gen plant than Columbia ecotype arabidopsis
It is sub big, and significant difference.It is consistent with the seed size of Columbia ecotype arabidopsis to turn the seed of empty carrier plant, without significantly
Difference.For the seed of harvest, apparent surface's projected area of the single seed of each strain（By Columbia ecotype
Surface projection's area of the seed of arabidopsis is as 100%）And relative weight（By the seed of Columbia ecotype arabidopsis
Weight is as 100%）See Fig. 3.The measuring method of surface projection's area of seed：Seed is kept flat（Cotyledon respectively has a piece of up and down）
In in plane, front shooting photo, surface projection of the obtained seed projection i.e. as seed, its area is the surface of seed
Projected area；Each strain from 500 seeds of 30 plant harvests to measuring, results averaged.The weight of seed
Measuring method：Each strain from 1500 seeds of 30 plant harvests to weighing, results averaged.
Plant photo after being cultivated 28 days in soil property culture medium is shown in Fig. 2 B.The mature leaf of transfer-gen plant is than brother rival
The mature leaf of sub- Arabidopsis thaliana ecotype is big, and significant difference.The blade and Columbia ecotype for turning empty carrier plant intend south
The leaf blade size of mustard is consistent, no significant difference.The 5th true leaf of plant（Maturity period）Relative vane area（Colombia is given birth to
5th true leaf of state type arabidopsis（Maturity period）Area as 100%）With relative vane cell area（Colombia is given birth to
The blade cell area of state type arabidopsis is as 100%）See Fig. 4（Each strain is surveyed to the 5th true leaf of 30 plant
Amount, results averaged）.The measuring method of blade cell area：Blade is taken, first in transparent liquid（80g chloraldurates, 30ml water
Mixed with 10ml glycerine）Middle soaking at room temperature 3 days, then tabletting and under DIC microscopes shoot cell photo, with ImageJ softwares
Build measurement cell area（The cell area at cell area and base portion 1/4 at every crop leaf measuring top 1/4, both add and
Average again）.As a result show, the blade area of the 5th true leaf of transfer-gen plant is noticeably greater than Columbia ecotype plan
Southern mustard, the blade area for turning the 5th true leaf of empty carrier plant are not significantly different with Columbia ecotype arabidopsis.Knot
Fruit shows, transfer-gen plant, Columbia ecotype arabidopsis and the blade cell area that turns empty carrier plant do not have significance difference
It is different, therefore it may be speculated that the difference of blade area is as caused by number of cells difference, i.e. overexpression AtUBP15 genes promote
The cell propagation of plant.
During being cultivated in soil property culture medium, the photo in second colored maturity period on the stem of plant is shown in figure
2C.The maturation flower of transfer-gen plant is spent greatly than the maturation of Columbia ecotype arabidopsis, and significant difference.Turn empty carrier plant
Ripe flower and the maturation of Columbia ecotype arabidopsis spend in the same size, no significant difference.It is single on the stem of plant
Colored relative petal area（Using the petal area of the single flower of Columbia ecotype arabidopsis as 100%）, relative petal grows
Degree（Using the petal length of the single flower of Columbia ecotype arabidopsis as 100%）, relative petal width（By Colombia
The petal width of the single flower of Arabidopsis thaliana ecotype is as 100%）With relative petal cell area（Columbia ecotype is intended
The petal cell area of southern mustard is as 100%）See Fig. 5（Each strain to second on the stem of 30 plant to the 5th into
Ripe flower measures, results averaged）.The measuring method of petal cell area：Petal is taken, first the soaking at room temperature in transparent liquid
1 day, then tabletting and the shooting cell photo under DIC microscopes, cell area was measured with ImageJ softwares（Every petal detection
The cell area of the widest part）.As a result show, petal area, petal length and the petal width of transfer-gen plant are noticeably greater than
Columbia ecotype arabidopsis, petal area, petal length and the petal width for turning empty carrier plant are given birth to Colombia
State type arabidopsis is not significantly different.As a result show, transfer-gen plant, Columbia ecotype arabidopsis and turn empty carrier plant
Petal cell area be not significantly different, therefore it may be speculated that the difference of petal area is as caused by number of cells difference,
It is overexpressed the cell propagation that AtUBP15 genes promote plant.
Result above shows, with Columbia ecotype arabidopsis（Wild type）And turn empty carrier plant and compare, turn base
Because the seed, flower, blade of plant significantly become big, and enhanced cellular proliferation is to cause organ to become the reason for big.
Claims (6)
1. a kind of method for cultivating genetically modified plants, it is by the channel genes purpose plant for encoding AtUBP15 albumen, is sent out
Educate the horizontal genetically modified plants for being better than the purpose plant；" developmental level is excellent " is presented as that seed is big and/or seed weight
It is big and/or spend big and/or blade big and/or cell propagation is fast；
The AtUBP15 albumen is following (a) or (b)：
(a) protein being made up of the amino acid sequence shown in sequence in sequence table 1；
(b) protein being made up of the amino acid sequence shown in sequence in sequence table 2.
2. the method as described in claim 1, it is characterised in that：The gene of the coding AtUBP15 albumen be following (1) or
(2) DNA molecular：
(1) DNA molecular shown in the sequence 3 of sequence table；
(2) DNA molecular of the code area as shown in the sequence 4 of sequence table or the sequence 5 of sequence table.
3. the method as described in claim 1, it is characterised in that：The purpose plant is monocotyledon or dicotyledon.
The recombinant vector of 4.AtUBP15 albumen, the gene for encoding AtUBP15 albumen or the gene containing coding AtUBP15 albumen
Application in the excellent genetically modified plants of developmental level are cultivated；" developmental level is excellent " is presented as that seed is big and/or plants
Sub- weight is big and/or spends big and/or blade big and/or cell propagation is fast；
The AtUBP15 albumen is following (a) or (b)：
(a) protein being made up of the amino acid sequence shown in sequence in sequence table 1；
(b) protein being made up of the amino acid sequence shown in sequence in sequence table 2.
5. application as claimed in claim 4, it is characterised in that：The gene of the coding AtUBP15 albumen be following (1) or
(2) DNA molecular：
(1) DNA molecular shown in the sequence 3 of sequence table；
(2) DNA molecular of the code area as shown in the sequence 4 of sequence table or the sequence 5 of sequence table.
6. application as claimed in claim 4, it is characterised in that：The plant that sets out of the genetically modified plants for monocotyledon or
Dicotyledon.
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| CN102757486A (en) * | 2011-04-27 | 2012-10-31 | 中国农业大学 | Plant development related protein GA20ox, and encoding gene and application thereof |
| CN103224555A (en) * | 2013-05-27 | 2013-07-31 | 中国农业科学院棉花研究所 | Plant-development-related protein GhSOC1, and coding gene and application thereof |
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| CN103224555A (en) * | 2013-05-27 | 2013-07-31 | 中国农业科学院棉花研究所 | Plant-development-related protein GhSOC1, and coding gene and application thereof |
Non-Patent Citations (3)
| Title |
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| NP_001185019.1;Theologis A等;《Genbank》;20130605;第1页 * |
| NP_564014.1;Theologis A等;《Genbank》;20130605;第1页 * |
| Ubiquitin C-terminal hydrolases 1 and 2 affect shoot architecture in Arabidopsis;Peizhen yang等;《The plant Journal》;20071231;第51卷;第441-457页 * |
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