CN104849212B - Pinhole device and pinhole adjustment method - Google Patents
Pinhole device and pinhole adjustment method Download PDFInfo
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- CN104849212B CN104849212B CN201410056362.2A CN201410056362A CN104849212B CN 104849212 B CN104849212 B CN 104849212B CN 201410056362 A CN201410056362 A CN 201410056362A CN 104849212 B CN104849212 B CN 104849212B
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000003780 insertion Methods 0.000 claims description 5
- 230000037431 insertion Effects 0.000 claims description 5
- 238000003384 imaging method Methods 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 10
- 238000007334 copolymerization reaction Methods 0.000 description 8
- 238000013461 design Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000001218 confocal laser scanning microscopy Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000879 optical micrograph Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
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- 230000001360 synchronised effect Effects 0.000 description 1
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Abstract
The invention discloses a kind of pinhole devices, comprising: stepper motor, two movable sliders, two thin slices loading stage and two thin slices；The front end of the screw rod of stepper motor is provided with pin-shaped nut, and thin slice is arranged on thin slice loading stage, and thin slice loading stage is arranged on movable slider.It is all respectively arranged with a hole in two thin slices, is superimposed up and down by the hole of two thin slices and forms pin hole, and the size of pin hole is determined by the size of the overlap-add region in the hole of two thin slices；Pass through the linear motion of the pin-shaped nut of step motor control and make two movable sliders rotations, the rotation for passing through movable slider adjusts the size of pin hole.The invention also discloses a kind of pin hole adjusting methods.It is automatically controlled continuously adjustable that the present invention is able to achieve aperture size, and possesses accurately electrical feedback position capability, can obtain accurate pinhole size parameter, confocal system can be made to possess stronger imaging capability.
Description
Technical field
The present invention relates to the burnt micro- scanning systems of copolymerization, more particularly to a kind of pinhole device；The invention further relates to one kind
Pin hole adjusting method.
Background technique
Confocal system is widely used in optical microphotograph scanning field, wherein laser scanning co-focusing microscope (Laser
Scanning Confocal Microscope, LSCM) it is the laboratory apparatus that extensive utilization arrives in current field of biomedicine, tool
There is the advantages that high speed imaging, high spatial resolution, non-intervention lossless continuous optical chromatography, 3-D scanning, it has also become morphology divides
Strong research tool in the numerous areas such as daughter cell biology, material science.
It as shown in Figure 1A, is existing laser scanning co-focusing microscope schematic diagram one, which, which corresponds to, focuses feelings
Shape；It as shown in Figure 1B, is existing laser scanning co-focusing microscope schematic diagram two, which, which corresponds to, defocuses situation.It is existing
Having laser scanning co-focusing microscope includes: laser source 101, semi-transparent semi-reflecting lens 102, object lens 103, sample 104, pin hole
(pinhole) device 105 and light detection device 106.The laser source 101 is for emitting laser；Semi-transparent semi-reflecting lens 102 can be two
To Look mirror, in access of the laser from the laser source 101 to sample 104, semi-transparent semi-reflecting lens 102 are for making laser pass through and arrive
Up to object lens 103, object lens 103 are focused laser；In schematic diagram one, sample is placed exactly on the focal plane of object lens 103；Sample
Product 104 generate fluorescence under the effect of the laser, and fluorescence is focused by object lens 103 and in the other side of object lens 103, wherein half
Saturating semi-reflective mirror 102 reflects fluorescence so that fluorescent foci enables in this way in 105 position of pinhole device
Pinhole device 105 is passed through all to be filled by optical detection by the fluorescence that the sample on the focal plane of sample side of object lens 103 generates
Set 106 receptions.In schematic diagram two, sample 104 is not on the focal plane of object lens 103, and at this moment sample 104 is in laser irradiation
Under also generate fluorescence, but the fluorescence can not focus on 105 position of pinhole device after object lens 103, so non-
Fluorescence caused by sample 104 on the focal plane of object lens 103 can not cannot be filled by pinhole device 105 by optical detection
Set 106 receptions.
By Figure 1A and Figure 1B it is found that in confocal microscope system, image resolution ratio and signal of the pin hole to confocal system
Signal-to-noise ratio etc. plays a crucial role, and pin hole can adjust effectively, the hot spot of about light beams, when its pore size is arranged
In certain value, detector can receive only the light beam by pin hole, effective filter out non-focusing in-plane scatter light and gather
Stray light on focal plane other than non-focus spot obtains the signal relative to more high s/n ratio in common microscopic system.
The diameter of pin hole be typically provided to it is consistent with Airy (airydisk) diameter, by have 84% focus light onto up to visit
Device is surveyed, the signal-to-noise ratio of image is best.When Airy is that point light source passes through project verification lens imaging, formed due to diffraction in focal point
Hot spot.Center is bright round spot, around there is one group of weaker light and dark concentric annular striped, wherein dark with first
Ring is that the central bright spot of boundary is referred to as Airy.In order to meet this condition, in the design of pin hole, the range of pin hole usually exists
10 μm between 1mm, since the pin hole that has is close to 10 μm, aperture is very small, and cause to be difficult to after design can be effectively right with optical path
Standard, so the difficult point always in pinhole design.If pin hole can not be effectively matched with beam center, it will result in effectively
The loss of signal light and the infiltration of stray light.
Existing pinhole device is respectively provided with its shortcoming: the electronic pin hole runner of such as Thorlabs company, the pin hole
Built-in 16 pin holes of different sizes in device, when use, can only choose one of them closest to the pin hole of user demand, not have
There is degree of regulation selection, more accurate pinhole size can not be chosen.In another example the manual pin hole runner of JobinYvon company,
Although its manual adjustment system can adjust the size of pin hole, can not the adjusted pinhole size of accurate feedback, lead to nothing
Method captures accurate pin hole parameter information.These deficiencies make existing pinhole design scheme that can not provide more accurately needle
Pore size then constrains the imaging capability of confocal system to a certain extent.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of pinhole device, it is able to achieve that aperture size is automatically controlled continuously may be used
It adjusts, and possesses accurately electrical feedback position capability, accurate pinhole size parameter can be obtained, can confocal system be gathered around
There is stronger imaging capability.The present invention also provides a kind of pin hole adjusting methods of pinhole device.
In order to solve the above technical problems, pinhole device provided by the invention, comprising: stepper motor, two movable sliders, two
A thin slice loading stage and two thin slices.
The front end of the screw rod of the stepper motor is provided with pin-shaped nut, and pin-shaped nut described in the step motor control is done
Linear motion.
First side of two movable sliders is all disposed on same bearing, and two movable sliders can be around described
Bearing rotation.
Two thin slice loading stages are separately positioned on the movable slider and a thin slice loading stage corresponds to
One movable slider；Two thin slices are separately positioned on the thin slice loading stage and a thin slice corresponds to
One thin slice loading stage；Each movable slider drives the thin slice loading stage being arranged on and the thin slice to do phase
Same rotation.
It is all respectively arranged with a hole in two thin slices, is superimposed up and down by the hole of two thin slices and forms pin hole,
And the size of the pin hole is determined by the size of the overlap-add region in the hole of two thin slices.
Two movable sliders are connected with corresponding extension spring respectively, and the extension spring makes two when being not stretched
The movable slider is in initial position, and the extension spring is stretched when the corresponding movable slider rotates and generates one
Restoring force.
Second side of two movable sliders and the pin-shaped nut are corresponding, and the pin-shaped nut is from two work
Second side of movable slider is inserted into and rotates two movable sliders respectively, by controlling the pin-shaped nut insertion depth control
Make the rotational angle of two movable sliders, controlled by the rotational angle of two movable sliders of control it is two described thin
The size of the overlap-add region in the hole of piece is to adjust the aperture size.
A further improvement is that the shape in the hole being arranged in two thin slices is rectangular, round or ellipse.
A further improvement is that the size adjustable range of the pin hole is 10 microns to 1 millimeter.
A further improvement is that the pinhole device setting is in the burnt micro- scanning system of copolymerization, the pinhole device is used
In the scattering light on the focal plane of object lens for making the burnt micro- scanning system of the copolymerization by pinhole device arrival detector,
And the scattering light on the non-focal plane of the object lens is filtered out.
In order to solve the above technical problems, the pin hole adjusting method of pinhole device provided by the invention includes the following steps:
Step 1: the pin-shaped nut is not with two movable slider contacts, two movable sliders are in the drawing
It stretches in initial position under the action of spring, the pin hole is also at initial position.
Step 2: the linear motion moved forward or back is done by pin-shaped nut described in the step motor control, described
The pin-shaped nut enters two movable sliders from second side of two movable sliders under the control of stepper motor
Between and make two movable sliders rotations, pass through the control pin-shaped nut insertion depth and control two movable sliders
Rotational angle, the overlap-add region in the hole of two thin slices is controlled by the rotational angle of two movable sliders of control
Size is to adjust the aperture size.
Step 3: the pin-shaped nut is withdrawn into two movable sliders from second side of two movable sliders
Outside, two movable sliders are restored to initial position.
A further improvement is that the shape in the hole being arranged in two thin slices is rectangular, round or ellipse.
A further improvement is that the size adjustable range of the pin hole is 10 microns to 1 millimeter.
A further improvement is that the pinhole device setting is in the burnt micro- scanning system of copolymerization, the pinhole device is used
In the scattering light on the focal plane of object lens for making the burnt micro- scanning system of the copolymerization by pinhole device arrival detector,
And the scattering light on the non-focal plane of the object lens is filtered out.
The present invention compared with prior art, has following obvious prominent substantive distinguishing features and technical effect:
1, the present invention uses stepper motor as driving, realizes electric control, and can obtain and a little feed back, and is easy to be accurately positioned
And control.
2, the present invention is superimposed to form pin hole using the hole of two thin slices, and realizes needle using the propulsion of centrum, that is, pin-shaped nut
The big minor adjustment in hole it is continuously adjustable to be not only able to achieve aperture size, moreover it is possible to can be realized higher pin hole degree of regulation.
3, extension spring of the present invention is adjustable center when pin hole playbacks, and is equivalent to primary automatic school after playback every time
Standard, repetitive positioning accuracy with higher.
4, the present invention can preferably improve the percent of pass of signal light, inhibit stray light, enhance the light in confocal system
Learn focusing capability altogether.
Detailed description of the invention
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments:
Figure 1A is existing laser scanning co-focusing microscope schematic diagram one；
Figure 1B is existing laser scanning co-focusing microscope schematic diagram two；
Fig. 2 is pinhole device longitudinal section structural drawing of the embodiment of the present invention；
Fig. 3 is the top view of pinhole device of the embodiment of the present invention；
Fig. 4 is the right view of pinhole device of the embodiment of the present invention；
Fig. 5 is the perspective view of pinhole device of the embodiment of the present invention；
Fig. 6 A is the thin slice schematic diagram of pinhole device of the embodiment of the present invention；
Fig. 6 B is the pin hole schematic diagram of pinhole device of the embodiment of the present invention.
Specific embodiment
It as described in Figure 2, is pinhole device longitudinal section structural drawing of the embodiment of the present invention；It as described in Figure 3, is the embodiment of the present invention
The top view of pinhole device；It as described in Figure 4, is the right view of pinhole device of the embodiment of the present invention；It as described in Figure 5, is the present invention
The perspective view of embodiment pinhole device；It is 12 schematic diagram of thin slice of pinhole device of the embodiment of the present invention as described in Fig. 6 A；Such as Fig. 6 B
It is described, it is 14 schematic diagram of pin hole of pinhole device of the embodiment of the present invention.
Pinhole device of the embodiment of the present invention includes: 11, two movable sliders 2, two thin slices loading stages 3 and two of stepper motor
A thin slice 12.
The front end of the screw rod of the stepper motor 11 is provided with pin-shaped nut 1, and the stepper motor 11 controls described pin-shaped
Nut 1 moves in a straight line.The top of the pin-shaped nut 1 be it is pin-shaped, also the thickness of the top-to-bottom of as pin-shaped nut 1 by
It is decrescence few, but the side of pin-shaped nut 1 is a flat surface.The rear end of the screw rod of the stepper motor 11 installs 4 He of compressed spring additional
Fixture nut 10.
First side of two movable sliders 2 is all disposed on same bearing 5, and two movable sliders 2 can be around
The bearing 5 rotates.
Two thin slice loading stages 3 are separately positioned on the movable slider 2 and a thin slice loading stage 3 is right
The Ying Yuyi movable sliders 2；Two thin slices 12 are separately positioned on the thin slice loading stage 3 and one described thin
Piece 12 corresponds to a thin slice loading stage 3；Each movable slider 2 drives the thin slice loading stage 3 being arranged on
Identical rotation is done with the thin slice 12.
As shown in Figure 6A, be all respectively arranged with a hole 13 in two thin slices 12, the shape in the hole 13 be it is rectangular,
Round or ellipse.
As shown in Figure 6B, pin hole 14 is formed by about the 13 hole superposition of two thin slices 12, and the pin hole 14 is big
The size of the overlap-add region in the small hole 13 by two thin slices 12 determines；Such as when two holes 13 are superimposed completely, at this moment pin hole
14 size is maximum and is the size in hole 13, and when two 13 overlap-add regions of hole become smaller, the size of pin hole 14 is gradually become smaller；So
The embodiment of the present invention can be realized continuously adjusting for the size of pin hole 14.Preferably, the size adjustable range of the pin hole 14 is
10 microns to 1 millimeter.
Two movable sliders 2 are connected with corresponding extension spring respectively, and the extension spring makes two when being not stretched
A movable slider 2 is in initial position, and the extension spring is stretched and produces when the corresponding movable slider 2 rotates
A raw restoring force.Two movable sliders 2 are in when initial position playbacks corresponding to pin hole 14 in the embodiment of the present invention
Heart position.Many places spring is used in the embodiment of the present invention, such as extension spring 7, extension spring 8 and extension spring 9, to help to live
Dynamic sliding shoe 2 possesses the pulling force reversed with pin-shaped nut 1, makes it when pin-shaped nut 1 promotes, can be fully synchronized in the step of motor
Into, and when pin-shaped nut 1 exits, help pin hole 14 to revert to center.
Second side of two movable sliders 2 and the pin-shaped nut 1 are corresponding, and the pin-shaped nut 1 is from two institutes
The second side for stating movable slider 2 is inserted into and rotates two movable sliders 2 respectively, is inserted by controlling the pin-shaped nut 1
Enter the rotational angle of deep-controlled two movable sliders 2, controlled by the rotational angle of two movable sliders 2 of control
The size of the overlap-add region in the hole 13 of two thin slices 12 is to adjust 14 size of pin hole.
Preferably, the pinhole device setting of the embodiment of the present invention is in the burnt micro- scanning system of copolymerization, the pin hole
Device is used to make the scattering light on the focal plane of the object lens of the burnt micro- scanning system of the copolymerization to reach by the pinhole device
Detector simultaneously filters out the scattering light on the non-focal plane of the object lens.The burnt micro- scanning system of copolymerization can be as shown in Figure 1A
Laser scanning co-focusing microscope, the pinhole device 105 in Figure 1A is replaced with the pinhole device of the embodiment of the present invention.
The pin hole adjusting method of pinhole device of the embodiment of the present invention includes the following steps:
Step 1: the pin-shaped nut 1 is not contacted with two movable sliders 2, two movable sliders 2 are described
Initial position is under the action of extension spring, the pin hole 14 is also at initial position.
Step 2: controlling the pin-shaped nut 1 by the stepper motor 11 does the linear motion moved forward or back, in institute
It states under the control of stepper motor 11 the pin-shaped nut 1 and enters two work from second side of two movable sliders 2
Between movable slider 2 and two movable sliders 2 are rotated, controls two institutes by controlling pin-shaped 1 insertion depth of nut
The rotational angle for stating movable slider 2 controls two thin slices 12 by the rotational angle of two movable sliders 2 of control
The size of the overlap-add region in hole 13 is to adjust 14 size of pin hole.
Step 3: the pin-shaped nut 1 is withdrawn into two movable sliders from second side of two movable sliders 2
2 outside, two movable sliders 2 are restored to initial position.
The present invention has been described in detail through specific embodiments, but these are not constituted to limit of the invention
System.Without departing from the principles of the present invention, those skilled in the art can also make many modification and improvement, these are also answered
It is considered as protection scope of the present invention.
Claims (8)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410056362.2A CN104849212B (en) | 2014-02-19 | 2014-02-19 | Pinhole device and pinhole adjustment method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410056362.2A CN104849212B (en) | 2014-02-19 | 2014-02-19 | Pinhole device and pinhole adjustment method |
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| Publication Number | Publication Date |
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| CN104849212A CN104849212A (en) | 2015-08-19 |
| CN104849212B true CN104849212B (en) | 2019-04-23 |
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| CN201410056362.2A Expired - Fee Related CN104849212B (en) | 2014-02-19 | 2014-02-19 | Pinhole device and pinhole adjustment method |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107229114A (en) * | 2017-04-12 | 2017-10-03 | 上海戴泽光电科技有限公司 | Multichannel fluorescence detection system based on fiber array |
| CN108387562B (en) * | 2018-02-02 | 2020-09-15 | 中国科学院上海光学精密机械研究所 | Adjustment method of pinhole axial position in confocal microscope system |
| JP7078115B2 (en) * | 2018-07-26 | 2022-05-31 | 株式会社島津製作所 | Light scattering detector |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10147481A1 (en) * | 2001-09-26 | 2003-04-17 | Zeiss Carl Jena Gmbh | Pinhole shutter for laser scanning microscopy has a simple cost-effective cam arrangement that provides accurate adjustment of size and position of the pinhole |
| WO2004104522A1 (en) * | 2003-05-22 | 2004-12-02 | Carl Zeiss Jena Gmbh | Adjustable pinhole in particular for a laser-scanning microscope |
| WO2013133767A1 (en) * | 2012-03-07 | 2013-09-12 | National University Of Singapore | A mems iris diaphragm for an optical system and method for adjusting a size of an aperture thereof |
| CN103698880A (en) * | 2013-12-26 | 2014-04-02 | 中国科学院苏州生物医学工程技术研究所 | Electric real-time adjustment method of two-dimensional pinhole for confocal laser scanning microscope |
-
2014
- 2014-02-19 CN CN201410056362.2A patent/CN104849212B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10147481A1 (en) * | 2001-09-26 | 2003-04-17 | Zeiss Carl Jena Gmbh | Pinhole shutter for laser scanning microscopy has a simple cost-effective cam arrangement that provides accurate adjustment of size and position of the pinhole |
| WO2004104522A1 (en) * | 2003-05-22 | 2004-12-02 | Carl Zeiss Jena Gmbh | Adjustable pinhole in particular for a laser-scanning microscope |
| WO2013133767A1 (en) * | 2012-03-07 | 2013-09-12 | National University Of Singapore | A mems iris diaphragm for an optical system and method for adjusting a size of an aperture thereof |
| CN103698880A (en) * | 2013-12-26 | 2014-04-02 | 中国科学院苏州生物医学工程技术研究所 | Electric real-time adjustment method of two-dimensional pinhole for confocal laser scanning microscope |
Non-Patent Citations (2)
| Title |
|---|
| A virtual size-variable pinhole for single photon confocal microscopy;Guangjun Gao et al;《Proc. of SPIE》;20131231;第8573卷;第1-8页 * |
| 空间针孔滤波器的使用技巧;黄建群等;《物理实验》;20031231;第23卷(第10期);第35-38页 * |
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