CN104845865B - A kind of constant temperature overflow mechanism applied in Rumen simulation is studied - Google Patents
A kind of constant temperature overflow mechanism applied in Rumen simulation is studied Download PDFInfo
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- CN104845865B CN104845865B CN201510325740.7A CN201510325740A CN104845865B CN 104845865 B CN104845865 B CN 104845865B CN 201510325740 A CN201510325740 A CN 201510325740A CN 104845865 B CN104845865 B CN 104845865B
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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Abstract
The invention provides a kind of constant temperature overflow mechanism applied in Rumen simulation is studied, described overflow mechanism includes that overflowing liquid receives pipe and overflow tank；Described overflowing liquid receives pipe and stretches into inside described overflow tank, for receiving the overflowing liquid from fermentation tank；The tank skin of described overflow tank is double-decker, is divided into inner and outer wall；Described overflow tank includes coolant inlet and the cooling liquid outlet being arranged at described overflow tank outer wall；Coolant can be injected in described double-decker by described coolant inlet and described cooling liquid outlet, and keep described coolant to circulate.By in the present invention apply Rumen simulation study in constant temperature overflow mechanism, it is able to receive that the liquid overflowed after fermentation substrate fermentation in Rumen simulation fermentation tank and is maintained at constant low temperature state, thus avoid overflowing liquid to continue fermentation, obtain close to the output product after Rumen simulation ferment tank.
Description
Technical field
The present invention relates to ruminant animal nutrition studying technological domain, apply at cud mould particularly to one
Intend the constant temperature overflow mechanism in research.
Background technology
Along with the continuous growth of China's ruminant quantity, China gradually faces high quality forage shortage of resources
Problem.In order to alleviate the problem of high quality forage shortage of resources, need the cud of ruminant is ground
Study carefully, to seek to be suitable for the high quality forage succedaneum of ruminant.
At present, the cud of in-vitro cultivation systematic study ruminant is mainly used.Existing
In-vitro cultivation system in, generally use fermentation tank simulation rumen that fermentation substrate is fermented,
And use common vessel to take up the overflowing liquid overflowed after fermentation substrate ferments.In order to prevent entering in container
Overflowing liquid continue fermentation, need to make the container of reception overflowing liquid be maintained at constant low temperature state.
Existing container cannot make overflowing liquid be maintained at constant low temperature state so that the overflowing liquid in container
Continue fermentation, thus output product after the fermentation of Rumen simulation fermentation tank cannot be refined.
Summary of the invention
The invention provides a kind of constant temperature overflow mechanism applied in Rumen simulation is studied, it is possible to receive
Overflowing liquid that Rumen simulation fermentation tank is discharged also is maintained at constant low temperature state, thus avoids overflowing liquid to continue
Supervention ferment, obtains close to the output product after Rumen simulation ferment tank.
First aspect, embodiments provides a kind of constant temperature applied in Rumen simulation is studied and overflows
Stream device, described overflow mechanism includes that overflowing liquid receives pipe and overflow tank；
Described overflowing liquid receives pipe and stretches into inside described overflow tank, for receiving the overflow from fermentation tank
Liquid；
The tank skin of described overflow tank is double-decker, is divided into inner and outer wall；
Described overflow tank includes coolant inlet and the cooling liquid outlet being arranged at described overflow tank outer wall；
Can inject cold in described double-decker by described coolant inlet and described cooling liquid outlet
But liquid, and keep described coolant to circulate；
It is additionally provided with liquid-level switch, when the liquid level of the overflowing liquid in described overflow tank inside described overflow tank
When rising to predeterminated position, described liquid-level switch controls the overflowing liquid in described overflow tank and flows out.
In conjunction with first aspect, embodiments provide the first possible embodiment party of first aspect
Formula, wherein, described overflow mechanism also includes sealing, with described overflow tank, the sealing-plug coordinated；
Described overflowing liquid receives pipe and coordinates with the sealing of described sealing-plug, enters described through described sealing-plug
Inside overflow tank.
In conjunction with the first possible embodiment of first aspect, embodiments provide first aspect
The possible embodiment of the second, wherein, described liquid-level switch enters described through described sealing-plug
Inside overflow tank.
In conjunction with the embodiment that first aspect the second is possible, embodiments provide first aspect
The third possible embodiment, wherein, the bottom of described overflow tank is provided with overflow box outlet；
Described overflow box outlet runs through the inner and outer wall of described overflow tank；
Described overflow box outlet is connected with pinch valve by conduit, and described conduit prolongs from described pinch valve
Stretch.
In conjunction with the third possible embodiment of first aspect, embodiments provide first aspect
The 4th kind of possible embodiment, wherein, described conduit is silica gel hose.
In conjunction with the third possible embodiment of first aspect, embodiments provide first aspect
The 5th kind of possible embodiment, wherein, when the liquid level of the overflowing liquid in described overflow tank rises to
During predeterminated position, described liquid-level switch turns on, and triggers described pinch valve and opens, inside described overflow tank
Overflowing liquid flowed out by described conduit.
In conjunction with the third possible embodiment of first aspect, embodiments provide first aspect
The 6th kind of possible embodiment, wherein, the tank skin of described overflow tank is provided with liquid phase thief hatch；
Described liquid phase thief hatch runs through the inner and outer wall of described overflow tank, is used for gathering described overflow tank
Interior overflowing liquid.
In conjunction with first aspect, embodiments provide the 7th kind of possible embodiment party of first aspect
Formula, wherein, described overflow mechanism also includes being arranged at the coolant generator outside described overflow filling,
Described coolant generator carries coolant to described coolant inlet, and reclaims from described coolant
The coolant of entrance.
In conjunction with the 7th kind of possible embodiment of first aspect, embodiments provide first aspect
The 8th kind of possible embodiment, wherein, described coolant generator includes the low temperature being interconnected
Water pump and constant temperature water bath, described low temperature water pump connects with described coolant inlet, described water bath with thermostatic control
Groove connects with described cooling liquid outlet.
In conjunction with first aspect, embodiments provide the 9th kind of possible embodiment party of first aspect
Formula, wherein, described double-decker is welded by clear glass.
In the embodiment of the present invention, receive pipe by overflowing liquid and be able to receive that the overflowing liquid from fermentation tank.
The tank skin of overflow tank is double-decker, can be upwards by the coolant inlet being arranged at overflow tank outer wall
State injection coolant in double-decker, can discharge by being arranged at the cooling liquid outlet of overflow tank outer wall
Above-mentioned coolant so that the coolant in double-decker keeps continuing circulation status, so that overflow tank
Kept constant low temperature state by the cooling effect of coolant always.Therefore by the embodiment of the present invention
Constant temperature overflow mechanism, it is possible to receive Rumen simulation fermentation tank discharge overflowing liquid and be maintained at constant low
Temperature state, thus avoid overflowing liquid to continue fermentation, thus obtain after Rumen simulation ferment tank
Output product.
Accompanying drawing explanation
In order to be illustrated more clearly that the technical scheme of the embodiment of the present invention, below will be to required in embodiment
Accompanying drawing to be used is briefly described, it will be appreciated that the following drawings illustrate only some of the present invention
Embodiment, is therefore not construed as the restriction to scope, for those of ordinary skill in the art,
On the premise of not paying creative work, it is also possible to obtain other relevant accompanying drawings according to these accompanying drawings.
Fig. 1 illustrates a kind of structural representation of the overflow mechanism that the embodiment of the present invention provided；
Fig. 2 illustrates the another kind of structural representation of the overflow mechanism that the embodiment of the present invention provided.
Reference:
Overflowing liquid receives pipe 1, overflow tank 2, inwall 21, outer wall 22, coolant inlet 3, coolant
Outlet 4, sealing-plug 5, liquid-level switch 6, overflow box outlet 7, conduit 8, pinch valve 9, liquid phase is adopted
Sample mouth 10, low temperature water pump 11, constant temperature water bath 12.
Detailed description of the invention
It should be noted that in the case of not conflicting, in embodiment in the application and embodiment
Feature can be mutually combined.Describe the present invention below with reference to the accompanying drawings and in conjunction with the embodiments in detail.
In order to make those skilled in the art be more fully understood that the present invention program, below in conjunction with the present invention
Accompanying drawing in embodiment, is clearly and completely described the technical scheme in the embodiment of the present invention, aobvious
So, described embodiment is only a part of embodiment of the present invention rather than whole embodiments.
Generally herein described in accompanying drawing and the assembly of the embodiment of the present invention that illustrates can be with various different joining
Put and arrange and design.Therefore, retouching in detail the embodiments of the invention provided in the accompanying drawings below
State the scope being not intended to limit claimed invention, but be merely representative of the selected reality of the present invention
Execute example.Based on embodiments of the invention, those skilled in the art are not before making creative work
Put the every other embodiment obtained, broadly fall into the scope of protection of the invention.
For ease of understanding the use scene of the present invention, the most briefly introduce.Rumen zymosis is being entered
During row research, fermentation tank simulation rumen is generally used to ferment.The material deposited in fermentation tank has:
Fermentation substrate and the rumen fluid of inoculation.When carrying out cud research, additionally it is possible to add it in fermentation tank
He is about the material of rumen zymosis, and these those skilled in the art will appreciate that, the thinnest
Lift.
After content fermentation in fermentation tank, liquid phase fermentation chyme and solid phase fermentation chyme can be produced, again
Liquid phase tunning and solid phase tunning, or liquid phase chyme, solid phase chyme can be called.Wherein
Liquid phase fermented material be a kind of liquid fermenting and producing, can by other container reception in research process.
Those skilled in the art can be apparent from the common product after rumen zymosis and common name thereof, this
In illustrate the most one by one.
Due to liquid phase fermentation chyme be the form overflowing fermentation tank by other container reception, therefore by
The liquid phase fermentation chyme received can be called again overflowing liquid.Skilled artisans appreciate that overflowing liquid
Implication and source.
Based on content described above, those skilled in the art are it should be understood that relevant art in the application
Language, such as " content ", " liquid phase fermentation chyme " etc..
The state of constant low temperature cannot be maintained at so that the overflow in overflow tank for existing overflow tank
Liquid continues fermentation, thus cannot refine the problem close to the output product after Rumen simulation ferment tank,
The invention provides a kind of constant temperature overflow mechanism applied in Rumen simulation is studied, it is possible to receive cud
Simulate the overflowing liquid of fermentation tank discharge and be maintained at constant low temperature state, thus avoiding overflowing liquid to continue to send out
Ferment, obtains close to the output product after Rumen simulation ferment tank.It is described below in conjunction with Fig. 1.
As it is shown in figure 1, embodiments provide a kind of constant temperature applied in Rumen simulation is studied
Overflow mechanism, this overflow mechanism includes that overflowing liquid receives pipe 1 and overflow tank 2；
Overflowing liquid receives pipe 1 and stretches into overflow tank 2 inside, for receiving the overflowing liquid from fermentation tank；
The tank skin of overflow tank 2 is double-decker, is divided into inwall 21 and outer wall 22；
Overflow tank 2 includes coolant inlet 3 and the cooling liquid outlet 4 being arranged at overflow tank outer wall 22；
Coolant can be injected in above-mentioned double-decker by coolant inlet 3 and cooling liquid outlet 4,
And keep coolant to circulate.
Visible, in the embodiment of the present invention, receive pipe 1 by overflowing liquid and be able to receive that from fermentation tank
Overflowing liquid.The tank skin of overflow tank 2 is double-decker, by being arranged at the coolant of overflow tank outer wall 22
Entrance 3 can inject coolant, by being arranged at the cold of overflow tank outer wall 22 in above-mentioned double-decker
But liquid outlet 4 can discharge above-mentioned coolant so that the coolant in double-decker keeps persistently circulating
State, so that overflow tank 2 is kept constant low temperature state by the cooling effect of coolant always.Cause
This is by the constant temperature overflow mechanism in the embodiment of the present invention, it is possible to receive what Rumen simulation fermentation tank was discharged
Overflowing liquid is also maintained at constant low temperature state, thus avoids overflowing liquid to continue fermentation, obtains close to cud
Output product after simulation ferment tank.
In the present embodiment, in order to make the overflowing liquid in entrance overflow tank 2 stop rapidly fermentation, the coldest
But the temperature of liquid is 4 degrees Celsius, and preferably coolant is the cold water of 4 degrees Celsius.
As it is shown in figure 1, coolant inlet 3 is arranged at the bottom of overflow tank 2, cooling liquid outlet 4 sets
It is placed in the top of overflow tank 2.
Coolant inlet 3 is relevant with the flow velocity of coolant with the openings of sizes of coolant inlet 4, specifically
Openings of sizes can be in conjunction with being actually needed decision.
As it is shown in figure 1, the overflow mechanism in the embodiment of the present invention also includes sealing with overflow tank 2 coordinating
Sealing-plug 5, sealing-plug 5 can be rubber stopper.In Fig. 1, overflowing liquid receives pipe 1 and sealing-plug 5
Seal and coordinate, thus keep the sealing of overflow tank 2 internal medium.Overflowing liquid receives pipe 1 through close
It is internal that blocking 5 enters overflow tank 2, thus is delivered in overflow tank 2 by the overflowing liquid from fermentation tank.
It can be silica gel hose that overflowing liquid receives pipe 1.
As it is shown in figure 1, the overflow mechanism in the embodiment of the present invention also includes liquid-level switch 6, liquid-level switch
6 enter the inside of overflow tank 2 through sealing-plug 5.
Specifically, the top half of liquid-level switch 6 seals with sealing-plug 5 and coordinates, thus ensures overflow
Seal in tank 2.
Liquid-level switch 6 is for detecting the liquid level in overflow tank 2, when liquid level reaches default height,
Liquid-level switch 6 starts, and triggering following operates.
As it is shown in figure 1, the overflow mechanism in the embodiment of the present invention also includes overflow box outlet 7, overflowing liquid
Outlet 7 is arranged at the bottom of overflow tank 2, and overflow box outlet 7 runs through the inner and outer wall of overflow tank 2,
Overflowing liquid in discharging overflow tank 2.
Overflow box outlet 7 is connected with pinch valve 9 by conduit 8, and conduit 8 is preferably silica gel hose.
From Fig. 1 it can be seen that conduit 8 extends from pinch valve 9, can be arranged by conduit 8
Go out the liquid in overflow tank 2.
In Fig. 1, the specific works process of liquid-level switch 6 and pinch valve 9 is as follows.
When the liquid level of the overflowing liquid in overflow tank 2 rises to predeterminated position, liquid-level switch 6 turns on,
Trigger pinch valve 9 to open, so that the overflowing liquid within overflow tank 2 is flowed out by conduit 8.
In the present embodiment, the double-decker of overflow tank tank skin preferably employs clear glass and is welded, and makes
Obtain experimenter and can observe the liquid condition in overflow tank 2, convenient record experimentation from the external world.
Glass material is stable, and intensity hardness meets use demand, uses glass material to make double-decker,
Enable to overflow tank durable stable.
The shape of overflow tank 2 can determine according to actual needs, and preferably overflow tank 2 is cylindrical structural.
As it is shown in figure 1, the overflow mechanism in the present embodiment also includes liquid phase thief hatch 10, liquid phase is sampled
Mouth 10 is arranged on overflow tank tank skin, runs through the inner and outer wall of overflow tank 2.Liquid phase thief hatch 10
Overflowing liquid in gathering overflow tank 2, in order to carry out subsequent analysis.
In order to further illustrate the overflow mechanism in the embodiment of the present invention, do further here in conjunction with Fig. 2
Explanation.
Overflow mechanism in the present embodiment also includes the coolant generator being arranged at outside overflow tank 2,
This coolant generator connects with coolant inlet, and this coolant generator can be to coolant inlet 3
Conveying coolant, additionally it is possible to reclaim the coolant from coolant inlet 4.
As in figure 2 it is shown, the coolant generator in the present embodiment includes low temperature water pump 11 and water bath with thermostatic control
Groove 12, this low temperature water pump 11 and constant temperature water bath 12 are interconnected.
In Fig. 2, low temperature water pump 11 is connected with coolant inlet 3 by sebific duct, thus enters for coolant
Mouth 3 conveying coolants.Constant temperature water bath 12 is connected with cooling liquid outlet 4 by sebific duct, thus reclaims
The coolant that cooling liquid outlet 4 is discharged.
In Fig. 2, after constant temperature water bath 12 reclaims the coolant that cooling liquid outlet 4 is discharged, it is possible to by it
It is delivered to low temperature water pump 11, under the effect of low temperature water pump 11, the coolant that recovery obtains is changed again
Become cryogenic liquid, thus be again delivered to coolant inlet 3, form the recycling of coolant.
Low temperature water pump 11 is not limited to sebific duct with the connection of coolant inlet 3, can select according to actual needs
Select.
Constant temperature water bath 12 is preferably the cryogenic thermostat water bath of-10 degrees Celsius to 60 degrees Celsius, its with
The connection of coolant inlet 4 is not limited to sebific duct, can select according to actual needs.
By the low temperature water pump in Fig. 2 and constant temperature water bath, it is possible to endlessly to overflow tank 2 tank
The double-decker of wall carries satisfactory coolant, and reclaims the coolant of discharge so that overflow
Tank 2 maintains the low temperature state close with coolant temperature.
In the present embodiment, coolant is preferably the cooling water of 4 degrees Celsius.
In cud, the fermentation temperature of feedstuff is 39 degrees Celsius, and the overflowing liquid temperature therefore produced also is
39 degrees Celsius, when the overflowing liquids of 39 degrees Celsius flow in the overflow tank in the present embodiment, it is possible to cold
But be cooled to rapidly 4 degrees Celsius under the effect of liquid so that overflowing liquid in material rapidly stop send out
Ferment reacts, in order to experimenter collects close to the output product after Rumen simulation ferment tank, it is simple to
Follow-up study uses.
In summary, in the embodiment of the present invention, receive pipe 1 by overflowing liquid and be able to receive that from fermentation
The overflowing liquid of tank.The tank skin of overflow tank 2 is double-decker, by being arranged at the cold of overflow tank outer wall 22
But liquid entrance 3 can inject coolant, by being arranged at overflow tank outer wall 22 in above-mentioned double-decker
Cooling liquid outlet 4 can discharge above-mentioned coolant so that coolant in double-decker keeps continuing
Circulation status, so that overflow tank 2 is kept temperature constant state by the cooling effect of coolant always.Cause
This is by the constant temperature overflow mechanism in the embodiment of the present invention, it is possible to receives liquid phase digestion chyme and is maintained at
Constant low temperature state, thus obtain close to the output product after Rumen simulation ferment tank.
The working background of the overflow mechanism in order to be more fully understood that in the present embodiment, below to ruminal digestion
Briefly introduction is done in systematic research.
China is livestock products big producing countries, and China cattle, sheep, the output value of milk account for the animal husbandry gross output value
20.74%, ruminant cultivation occupies critical role in China's animal husbandry.In ruminant cultivation constantly
In the case of growth, China is but faced with high quality forage shortage of resources problem, particularly in southern area,
Shortage of resources phenomenon is more serious.Thus it is necessary to seek more high quality forage succedaneum, many institute's weeks
Knowing, each new type resource the most all must carry out the assessment of assessment of nutritional value, toxicity,
The determination etc. of addition.Test with animal carcasses, easily damage animal bodies, therefore can first use external
Evaluation and test analogy method, reduces animal injury.
The digestion of ruminant tumor gastric, relies primarily on the perch various microorganisms in cud and primary dynamic
The decomposition of the various compositions in daily ration and fermentation, research are proved by thing, the dry in feedstuff about 40%～
80% digests in cud, and wherein 80% is carbohydrate；There are about the Organic substance of 60%～80%,
The crude fat of 10%～100% also digests in cud, and the 23% of gross energy～87% also in cud
Digestion.The especially crude fibre in feedstuff, about 90% ferments in cud, and the most about 10% disappears at large intestine
Changing, hemicellulose seldom or does not finds at post-ruminal digestion.
Cud is as a biological fermentation tank, it should possesses and is suitable for population complexity, the tumor of enormous amount
The condition of stomach microorganism growth and breeding wherein, these conditions can occur along with the growth and breeding of microorganism
Respective change.But, the living environment of microorganism, including nutrient substance, temperature, acid-base value etc. again
Require the most stable, therefore, in cud various environmental factorss must be at any time again tight regulation and control it
In.
At present, the method for research cud mainly has three kinds, respectively intracorporal method, half intracorporal method and external
Method.
Intracorporal method refers to install permanence lymphoma stomach fistulization pipe at the left shank portion cud knapsack of animal, passes through fistula
Pipe gathers rumen content sample and carries out the evaluation of microbiological research or feed nutritive value.Intracorporal method
Research always measures feed nutritive value most common method, but to there is the test period long for it, ring
The shortcomings such as border condition is wayward, this makes the method not be suitable for large-scale Feed Evaluation, can only
As a kind of reference method.Half intracorporal method is primarily referred to as Nylon Bag, and the method is directly by feedstuff sample
Product load in Nylon Bag, are suspended in cud the digestion feelings measuring feed nutrient by rumen fistula
Condition.This method is widely used in evaluating feedstuff palliating degradation degree in cud, but this method needs band fistula
Ruminant, its measurement result is affected by multiple extraneous factor, cause measurement result variation bigger.
In vitro method refers to gather fresh cud chyme or rumen fluid, carries out micro-in the device of simulation rumen condition
Biological culture, so also known as artificial rumen method, for intracorporal method, half intracorporal method, in vitro method
Have simple to operate, time saving and energy saving, environmental condition is easily controlled, the feature such as reproducible, and,
It can measure substantial amounts of Feed Sample within a short period of time.This method is suitable for the conventional analysis of laboratory,
This is particularly important for increasingly sophisticated modern ruminant animal nutrition research.
At present, typical in vitro method mainly has batch culture and continuous culture method.
Batch culture is microbial inoculant thing and fermentation substrate disposably to be added in round, warp
After certain time cultivates, terminate to cultivate within the set time, therefore belong to again static fermentation, this method due to
The separation of substrate and product can not be realized, cause rumen zymosis environment to change, be therefore not suitable for
Long-term in vitro simulation fermentation test.Batch culture mainly has two-phase method, aerogenesis method and simply digests
Method etc..But, in-vitro cultivation method achieves continuously entering and eating of substrate (feedstuff) and buffer
The continuous discharge of rotten (solid phase and liquid phase), thus it is more nearly in vivo rumen zymosis situation, compared to
There is for batch culture the advantage of environment in simulation rumen more accurately.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention not office
Being limited to this, any those familiar with the art, can in the technical scope that the invention discloses
Readily occur in change or replace, all should contain within protection scope of the present invention.Therefore, the present invention
Protection domain should described be as the criterion with scope of the claims.
Claims (10)
1. the constant temperature overflow mechanism applied in Rumen simulation is studied, it is characterised in that described
Overflow mechanism includes that overflowing liquid receives pipe and overflow tank；
Described overflowing liquid receives pipe and stretches into inside described overflow tank, for receiving the overflow from fermentation tank
Liquid；
The tank skin of described overflow tank is double-decker, is divided into inner and outer wall；
Described overflow tank includes coolant inlet and the cooling liquid outlet being arranged at described overflow tank outer wall；
Can inject cold in described double-decker by described coolant inlet and described cooling liquid outlet
But liquid, and keep described coolant to circulate；
It is additionally provided with liquid-level switch, when the liquid level of the overflowing liquid in described overflow tank inside described overflow tank
When rising to predeterminated position, described liquid-level switch controls the overflowing liquid in described overflow tank and flows out.
Overflow mechanism the most according to claim 1, it is characterised in that described overflow mechanism is also
The sealing-plug coordinated is sealed including with described overflow tank；
Described overflowing liquid receives pipe and coordinates with the sealing of described sealing-plug, enters described through described sealing-plug
Inside overflow tank.
Overflow mechanism the most according to claim 2, it is characterised in that described liquid-level switch is worn
Cross described sealing-plug to enter inside described overflow tank.
Overflow mechanism the most according to claim 3, it is characterised in that the end of described overflow tank
Portion is provided with overflow box outlet；
Described overflow box outlet runs through the inner and outer wall of described overflow tank；
Described overflow box outlet is connected with pinch valve by conduit, and described conduit prolongs from described pinch valve
Stretch.
Overflow mechanism the most according to claim 4, it is characterised in that described conduit is silica gel
Flexible pipe.
Overflow mechanism the most according to claim 4, it is characterised in that
When the liquid level of the overflowing liquid in described overflow tank rises to predeterminated position, described liquid-level switch is led
Logical, trigger described pinch valve and open, the overflowing liquid within described overflow tank is flowed out by described conduit.
Overflow mechanism the most according to claim 4, it is characterised in that the tank of described overflow tank
Wall is provided with liquid phase thief hatch；
Described liquid phase thief hatch runs through the inner and outer wall of described overflow tank, is used for gathering described overflow tank
Interior overflowing liquid.
Overflow mechanism the most according to claim 1, it is characterised in that described overflow mechanism is also
Including the coolant generator being arranged at outside described overflow filling, described coolant generator is to described cold
But liquid entrance conveying coolant, and reclaim the coolant from described coolant inlet.
Overflow mechanism the most according to claim 8, it is characterised in that described coolant occurs
Device includes low temperature water pump and the constant temperature water bath being interconnected, and described low temperature water pump enters with described coolant
Mouth connection, described constant temperature water bath connects with described cooling liquid outlet.
Overflow mechanism the most according to claim 1, it is characterised in that described double-decker by
Clear glass is welded.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565673A (en) * | 2009-06-02 | 2009-10-28 | 扬州大学 | Intelligent bionic rumen fermenter |
| CN101701182A (en) * | 2009-12-01 | 2010-05-05 | 北京科润维德生物技术有限责任公司 | Microbe biogas adhesive membrane type anaerobic fermentation reactor and application thereof |
| CN102286359A (en) * | 2010-12-28 | 2011-12-21 | 中国农业科学院北京畜牧兽医研究所 | Solid-liquid-gas three phase flow-dividing rumen imitation continuous fermentation system and method |
| CN203807463U (en) * | 2014-01-16 | 2014-09-03 | 黄山市迎客松生物科技有限公司 | Cooling water recycling device for fermentation tank |
-
2015
- 2015-06-15 CN CN201510325740.7A patent/CN104845865B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565673A (en) * | 2009-06-02 | 2009-10-28 | 扬州大学 | Intelligent bionic rumen fermenter |
| CN101701182A (en) * | 2009-12-01 | 2010-05-05 | 北京科润维德生物技术有限责任公司 | Microbe biogas adhesive membrane type anaerobic fermentation reactor and application thereof |
| CN102286359A (en) * | 2010-12-28 | 2011-12-21 | 中国农业科学院北京畜牧兽医研究所 | Solid-liquid-gas three phase flow-dividing rumen imitation continuous fermentation system and method |
| CN203807463U (en) * | 2014-01-16 | 2014-09-03 | 黄山市迎客松生物科技有限公司 | Cooling water recycling device for fermentation tank |
Non-Patent Citations (1)
| Title |
|---|
| 双外流连续培养系统模拟瘤胃消化;夏兆刚 孟庆翔;《中国饲料》;20021208(第23期);第27－28页 * |
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