CN104845865A - Constant-temperature overflow device applied to rumen simulation research - Google Patents
Constant-temperature overflow device applied to rumen simulation research Download PDFInfo
- Publication number
- CN104845865A CN104845865A CN201510325740.7A CN201510325740A CN104845865A CN 104845865 A CN104845865 A CN 104845865A CN 201510325740 A CN201510325740 A CN 201510325740A CN 104845865 A CN104845865 A CN 104845865A
- Authority
- CN
- China
- Prior art keywords
- tank
- cooling fluid
- overflow device
- overflow
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Landscapes
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a constant-temperature overflow device applied to a rumen simulation research. The overflow device comprises an overflow liquid receiving tube and an overflow tank, wherein the overflow liquid receiving tube stretches in the overflow tank and is used for receiving an overflow liquid from a fermentation tank; the tank wall of the overflow tank is of a double-layer structure, and is divided into an inner wall and an outer wall; the overflow tank comprises a cooling liquid inlet and a cooling liquid outlet formed in the outer wall of the overflow tank; the cooling liquid inlet and the cooling liquid outlet are capable of injecting a cooling liquid in the double-layer structure, and keeping the flowing of the cooling liquid. Through the constant-temperature overflow device applied to the rumen simulation research disclosed by the invention, the liquid overflowing after a fermentation substrate in the fermentation tank for rumen simulation is fermented can be received and kept at a constant low-temperature state, thus preventing the overflow liquid from further being fermented to obtain a product similar to the product after the fermentation of the fermentation tank for rumen simulation.
Description
Technical field
The present invention relates to ruminant animal nutrition studying technological domain, particularly a kind of constant temperature overflow device be applied in Rumen simulation research.
Background technology
Along with the continuous growth of China's ruminating animal quantity, China faces high quality forage shortage of resources problem gradually.In order to alleviate the problem of high quality forage shortage of resources, need to study the cud of ruminating animal, to seek the high quality forage substitute being suitable for ruminating animal.
At present, the cud of in-vitro cultivation systematic study ruminating animal is mainly used in the world.In existing in-vitro cultivation system, fermentor tank simulation rumen is usually used to ferment to fermentation substrate, and the overflowing liquid overflowed after using common vessel to take up fermentation substrate fermentation.In order to prevent the overflowing liquid entered in container from continuing fermentation, need to make the container of reception overflowing liquid remain on constant low temperature state.
Existing container cannot make overflowing liquid remain on constant low temperature state, makes the overflowing liquid in container continue fermentation, thus output product after the fermentation close to Rumen simulation fermentor tank cannot be refined.
Summary of the invention
The invention provides a kind of constant temperature overflow device be applied in Rumen simulation research, the overflowing liquid of Rumen simulation fermentor tank discharge can be received and remain on constant low temperature state, thus avoid overflowing liquid to continue fermentation, obtain the output product after close to Rumen simulation ferment tank.
First aspect, embodiments provide a kind of constant temperature overflow device be applied in Rumen simulation research, described overflow device comprises overflowing liquid receiving tube and weir tank;
It is inner that described overflowing liquid receiving tube stretches into described weir tank, for receiving the overflowing liquid from fermentor tank;
The tank skin of described weir tank is bilayer structure, is divided into inner and outer wall;
Described weir tank comprises the cooling fluid entrance and cooling liquid outlet that are arranged at described weir tank outer wall;
Cooling fluid can be injected in described bilayer structure by described cooling fluid entrance and described cooling liquid outlet, and keep described cooling fluid to circulate.
In conjunction with first aspect, embodiments provide the first possible embodiment of first aspect, wherein, described overflow device also comprises the sealing plug be sealed and matched with described weir tank;
Described overflowing liquid receiving tube and described sealing plug are sealed and matched, and enter described weir tank inner through described sealing plug.
In conjunction with the first possible embodiment of first aspect, embodiments provide the embodiment that the second of first aspect is possible, wherein, described overflow device comprises liquid level switch, and it is inner that described liquid level switch enters described weir tank through described sealing plug.
In conjunction with the embodiment that first aspect the second is possible, embodiments provide the third possible embodiment of first aspect, wherein, the bottom of described weir tank is provided with overflow box outlet;
Described overflow box outlet runs through the inner and outer wall of described weir tank;
Described overflow box outlet is connected with pinch valve by conduit, and described conduit extends from described pinch valve.
In conjunction with the third possible embodiment of first aspect, embodiments provide the 4th kind of possible embodiment of first aspect, wherein, described conduit is silica gel hose.
In conjunction with the third possible embodiment of first aspect, embodiments provide the 5th kind of possible embodiment of first aspect, wherein, when the liquid level of the overflowing liquid in described weir tank rises to predeterminated position, described liquid level switch conducting, trigger described pinch valve to open, the overflowing liquid of described weir tank inside is flowed out by described conduit.
In conjunction with the third possible embodiment of first aspect, embodiments provide the 6th kind of possible embodiment of first aspect, wherein, the tank skin of described weir tank is provided with liquid phase thief hatch;
Described liquid phase thief hatch runs through the inner and outer wall of described weir tank, for gathering the overflowing liquid in described weir tank.
In conjunction with first aspect, embodiments provide the 7th kind of possible embodiment of first aspect, wherein, described overflow device also comprises the cooling fluid producer being arranged at described overflow filling outside, described cooling fluid producer to described cooling fluid entrance conveying cooling fluid, and retrieves the cooling fluid from described cooling fluid entrance.
In conjunction with first aspect the 7th kind of possible embodiment, embodiments provide the 8th kind of possible embodiment of first aspect, wherein, described cooling fluid producer comprises the low temperature water pump and constant temperature water bath that are interconnected, described low temperature water pump is communicated with described cooling fluid entrance, and described constant temperature water bath is communicated with described cooling liquid outlet.
In conjunction with first aspect, embodiments provide the 9th kind of possible embodiment of first aspect, wherein, described bilayer structure is welded by transparent glass.
In the embodiment of the present invention, the overflowing liquid from fermentor tank can be received by overflowing liquid receiving tube.The tank skin of weir tank is bilayer structure, cooling fluid can be injected in above-mentioned bilayer structure by the cooling fluid entrance being arranged at weir tank outer wall, above-mentioned cooling fluid can be discharged by the cooling liquid outlet being arranged at weir tank outer wall, make the cooling fluid in bilayer structure keep continuing circulation status, thus make weir tank keep constant low temperature state by the cooling effect of cooling fluid always.Therefore by the constant temperature overflow device in the embodiment of the present invention, the overflowing liquid of Rumen simulation fermentor tank discharge can be received and remain on constant low temperature state, thus avoiding overflowing liquid to continue fermentation, thus obtaining the output product after close to Rumen simulation ferment tank.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the embodiment of the present invention, be briefly described to the accompanying drawing used required in embodiment below, be to be understood that, the following drawings illustrate only some embodiment of the present invention, therefore the restriction to scope should be counted as, for those of ordinary skill in the art, under the prerequisite not paying creative work, other relevant accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 illustrates a kind of structural representation of the overflow device that the embodiment of the present invention provides;
Fig. 2 illustrates the another kind of structural representation of the overflow device that the embodiment of the present invention provides.
Reference numeral:
Overflowing liquid receiving tube 1, weir tank 2, inwall 21, outer wall 22, cooling fluid entrance 3, cooling liquid outlet 4, sealing plug 5, liquid level switch 6, overflow box outlet 7, conduit 8, pinch valve 9, liquid phase thief hatch 10, low temperature water pump 11, constant temperature water bath 12.
Embodiment
It should be noted that, when not conflicting, the embodiment in the application and the feature in embodiment can combine mutually.Below with reference to the accompanying drawings and describe the present invention in detail in conjunction with the embodiments.
The present invention program is understood better in order to make those skilled in the art person, below in conjunction with accompanying drawing in the embodiment of the present invention, technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.The assembly of the embodiment of the present invention describing and illustrate in usual accompanying drawing herein can be arranged with various different configuration and design.Therefore, below to the detailed description of the embodiments of the invention provided in the accompanying drawings and the claimed scope of the present invention of not intended to be limiting, but selected embodiment of the present invention is only represented.Based on embodiments of the invention, the every other embodiment that those skilled in the art obtain under the prerequisite not making creative work, all belongs to the scope of protection of the invention.
For ease of understanding use scenes of the present invention, briefly introduce here.When studying rumen zymosis, fermentor tank simulation rumen is usually used to ferment.The material deposited in fermentor tank has: the rumen fluid of fermentation substrate and inoculation.When carrying out cud research, can also add other materials about rumen zymosis in fermentor tank, these those skilled in the art can understand, and no longer carefully lift one by one here.
After content fermentation in fermentor tank, liquid phase fermentation chyme and solid phase fermentation chyme can be produced, liquid phase tunning and solid phase tunning can be called again, or liquid phase chyme, solid phase chyme.Liquid phase fermented material is wherein a kind of liquid produced that ferments, can by other container reception in research process.Those skilled in the art clearly can know the common product after rumen zymosis and common name thereof, illustrate no longer one by one here.
Because liquid phase fermentation chyme is the form of overflowing fermentor tank by other container reception, therefore received liquid phase fermentation chyme can be called overflowing liquid again.Those skilled in the art can understand implication and the source thereof of overflowing liquid.
Based on introducing content above, those skilled in the art should understand the relational language in the application, as " content ", " liquid phase fermentation chyme " etc.
The state of constant low temperature cannot be remained on for existing weir tank, the overflowing liquid in weir tank is made to continue fermentation, thus the problem of the output product after close to Rumen simulation ferment tank cannot be refined, the invention provides a kind of constant temperature overflow device be applied in Rumen simulation research, the overflowing liquid of Rumen simulation fermentor tank discharge can be received and remain on constant low temperature state, thus avoid overflowing liquid to continue fermentation, obtain the output product after close to Rumen simulation ferment tank.Be described below in conjunction with Fig. 1.
As shown in Figure 1, embodiments provide a kind of constant temperature overflow device be applied in Rumen simulation research, this overflow device comprises overflowing liquid receiving tube 1 and weir tank 2;
It is inner that overflowing liquid receiving tube 1 stretches into weir tank 2, for receiving the overflowing liquid from fermentor tank;
The tank skin of weir tank 2 is bilayer structure, is divided into inwall 21 and outer wall 22;
Weir tank 2 comprises the cooling fluid entrance 3 and cooling liquid outlet 4 that are arranged at weir tank outer wall 22;
Cooling fluid can be injected in above-mentioned bilayer structure by cooling fluid entrance 3 and cooling liquid outlet 4, and keep cooling fluid to circulate.
Visible, in the embodiment of the present invention, the overflowing liquid from fermentor tank can be received by overflowing liquid receiving tube 1.The tank skin of weir tank 2 is bilayer structure, cooling fluid can be injected in above-mentioned bilayer structure by the cooling fluid entrance 3 being arranged at weir tank outer wall 22, above-mentioned cooling fluid can be discharged by the cooling liquid outlet 4 being arranged at weir tank outer wall 22, make the cooling fluid in bilayer structure keep continuing circulation status, thus make weir tank 2 keep constant low temperature state by the cooling effect of cooling fluid always.Therefore by the constant temperature overflow device in the embodiment of the present invention, the overflowing liquid of Rumen simulation fermentor tank discharge can be received and remain on constant low temperature state, thus avoiding overflowing liquid to continue fermentation, obtaining the output product after close to Rumen simulation ferment tank.
In the present embodiment, in order to make the overflowing liquid entered in weir tank 2 stop rapidly fermentation, the temperature of preferred cooling fluid is 4 degrees Celsius, and preferred cooling fluid is the cold water of 4 degrees Celsius.
As shown in Figure 1, cooling fluid entrance 3 is arranged at the bottom of weir tank 2, and cooling liquid outlet 4 is arranged at the top of weir tank 2.
Cooling fluid entrance 3 is relevant with the flow velocity of cooling fluid with the openings of sizes of cooling fluid entrance 4, and concrete openings of sizes can determine in conjunction with actual needs.
As shown in Figure 1, the overflow device in the embodiment of the present invention also comprises the sealing plug 5 be sealed and matched with weir tank 2, and sealing plug 5 can be rubber plug.In Fig. 1, overflowing liquid receiving tube 1 and sealing plug 5 are sealed and matched, thus keep the stopping property of weir tank 2 internal medium.It is inner that overflowing liquid receiving tube 1 enters weir tank 2 through sealing plug 5, thus be delivered in weir tank 2 by the overflowing liquid from fermentor tank.
Overflowing liquid receiving tube 1 can be silica gel hose.
As shown in Figure 1, the overflow device in the embodiment of the present invention also comprises liquid level switch 6, and liquid level switch 6 enters the inside of weir tank 2 through sealing plug 5.
Particularly, upper part and the sealing plug 5 of liquid level switch 6 are sealed and matched, thus ensure the stopping property in weir tank 2.
Liquid level switch 6 is for detecting the liquid level in weir tank 2, and when liquid level reaches default height, liquid level switch 6 starts, and triggering following operates.
As shown in Figure 1, the overflow device in the embodiment of the present invention also comprises overflow box outlet 7, and overflow box outlet 7 is arranged at the bottom of weir tank 2, and overflow box outlet 7 runs through the inner and outer wall of weir tank 2, for discharging the overflowing liquid in weir tank 2.
Overflow box outlet 7 is connected with pinch valve 9 by conduit 8, and conduit 8 is preferably silica gel hose.
Can find out from Fig. 1, conduit 8 extends from pinch valve 9, can discharge the liquid in weir tank 2 by conduit 8.
In Fig. 1, the specific works process of liquid level switch 6 and pinch valve 9 is as follows.
When the liquid level of the overflowing liquid in weir tank 2 rises to predeterminated position, liquid level switch 6 conducting, triggers pinch valve 9 and opens, thus the overflowing liquid of weir tank 2 inside is flowed out by conduit 8.
In the present embodiment, the bilayer structure of weir tank tank skin preferably adopts transparent glass to be welded, and makes experimenter can observe liquid state in weir tank 2 from the external world, conveniently records experimentation.
Glass material is stablized, and intensity hardness meets user demand, uses glass material to make bilayer structure, can make weir tank durable stable.
The shape of weir tank 2 can determine according to actual needs, and preferred weir tank 2 is cylindrical structural.
As shown in Figure 1, the overflow device in the present embodiment also comprises liquid phase thief hatch 10, and liquid phase thief hatch 10 is arranged on weir tank tank skin, runs through the inner and outer wall of weir tank 2.Liquid phase thief hatch 10 for gathering the overflowing liquid in weir tank 2, to carry out subsequent analysis.
In order to further illustrate the overflow device in the embodiment of the present invention, composition graphs 2 is described further here.
Overflow device in the present embodiment also comprises the cooling fluid producer be arranged at outside weir tank 2, this cooling fluid producer is communicated with cooling fluid entrance, this cooling fluid producer can carry cooling fluid to cooling fluid entrance 3, can also retrieve the cooling fluid from cooling fluid entrance 4.
As shown in Figure 2, the cooling fluid producer in the present embodiment comprises low temperature water pump 11 and constant temperature water bath 12, and this low temperature water pump 11 and constant temperature water bath 12 are interconnected.
In Fig. 2, low temperature water pump 11 is communicated with cooling fluid entrance 3 by sebific duct, thus carries cooling fluid for cooling fluid entrance 3.Constant temperature water bath 12 is communicated with cooling liquid outlet 4 by sebific duct, thus reclaims the cooling fluid of cooling liquid outlet 4 discharge.
In Fig. 2, after constant temperature water bath 12 reclaims the cooling fluid of cooling liquid outlet 4 discharge, low temperature water pump 11 can be delivered to, again cryogenic liquid is transformed into by reclaiming the cooling fluid obtained under the effect of low temperature water pump 11, thus be again delivered to cooling fluid entrance 3, form recycling of cooling fluid.
Low temperature water pump 11 is not limited to sebific duct with the connection of cooling fluid entrance 3, can select according to actual needs.
Constant temperature water bath 12 is preferably the cryogenic thermostat water bath of-10 degrees Celsius to 60 degrees Celsius, and the connection of itself and cooling fluid entrance 4 is not limited to sebific duct, can select according to actual needs.
By the low temperature water pump in Fig. 2 and constant temperature water bath, satisfactory cooling fluid can be carried endlessly in the bilayer structure of weir tank 2 tank skin, and reclaim the cooling fluid of discharging, make the low-temperature condition that weir tank 2 remains close with coolant temperature.
In the present embodiment, cooling fluid is preferably the water coolant of 4 degrees Celsius.
In cud, the leavening temperature of feed is 39 degrees Celsius, therefore the overflowing liquid temperature produced also is 39 degrees Celsius, when flowing into when the overflowing liquids of 39 degrees Celsius in the weir tank in the present embodiment, 4 degrees Celsius can be cooled to rapidly under the effect of cooling fluid, thus the material in the overflowing liquid made stops fermentation reaction rapidly, so that experimenter collects the output product after close to Rumen simulation ferment tank, be convenient to follow-up study and use.
In summary, in the embodiment of the present invention, the overflowing liquid from fermentor tank can be received by overflowing liquid receiving tube 1.The tank skin of weir tank 2 is bilayer structure, cooling fluid can be injected in above-mentioned bilayer structure by the cooling fluid entrance 3 being arranged at weir tank outer wall 22, above-mentioned cooling fluid can be discharged by the cooling liquid outlet 4 being arranged at weir tank outer wall 22, make the cooling fluid in bilayer structure keep continuing circulation status, thus make weir tank 2 keep temperature constant state by the cooling effect of cooling fluid always.Therefore by the constant temperature overflow device in the embodiment of the present invention, can receiving liquid cancellation ratio chyme remain on constant low temperature state, thus obtain the output product after close to Rumen simulation ferment tank.
In order to understand the working background of the overflow device in the present embodiment better, below ruminal digestion systematic research is briefly introduced.
China is livestock product big producing countries, and the output value of China ox, sheep, milk accounts for 20.74% of the livestock industry gross output value, and ruminating animal cultivation occupies critical role in China's livestock industry.Under ruminating animal cultivates ever-increasing situation, China is but faced with high quality forage shortage of resources problem, and particularly in southern area, shortage of resources phenomenon is more serious.Thus be necessary to seek more high quality forage substitute, as everyone knows, each new type resource all must carry out the assessment of assessment of nutritional value, toxicity before application, the determination etc. of addition.With animal carcasses test, easily damage animal bodies, therefore first can use external evaluation and test analogy method, reduce animal injury.
The digestion of ruminant tumor gastric, the various microorganism of main dependence perch in cud and protozoon are to the decomposition of the various compositions in daily ration and fermentation, research proves, the dry-matter in feed about 40% ~ 80% digests in cud, and wherein 80% is carbohydrate; About have the organism of 60% ~ 80%, the crude fat of 10% ~ 100% also digests in cud, and 23% ~ 87% of total energy also digests in cud.Especially the robust fibre in feed, about 90% ferments in cud, and only about 10% in large intestinal digestion, and hemicellulose seldom or do not find at post-ruminal digestion.
Cud, as a biological fermentation tank, should possess the condition of rumen microorganism growth and breeding being wherein suitable for population complexity, enormous amount, and these conditions respective change can occur along with microbial growth breeding.But the living environment of microorganism, comprise nutritive substance, temperature, potential of hydrogen etc. and require again relatively stable, therefore, in cud, various environmental factors must be in again among tight regulation and control at any time.
At present, the method for research cud mainly contains three kinds, is respectively intracorporal method, half intracorporal method and in vitro method.
Intracorporal method refers at the cud knapsack place of left shank portion of animal installation permanence lymphoma stomach fistulization pipe, gathers by fistula the evaluation that rumen content sample carries out microbiological research or feed nutritive value.Intracorporal method research is measure the most frequently used method of feed nutritive value always, but to there is the test period long for it, and the shortcomings such as envrionment conditions is wayward, this makes the method not be suitable for large-scale Feed Evaluation, can only as a kind of reference method.Half intracorporal method mainly refers to Nylon Bag, and the method directly loads in Nylon Bag by Feed Sample, is suspended in cud the digestion situation measuring feed nutrient by rumen fistula.This method is widely used in evaluating the palliating degradation degree of feed in cud, but this method needs the ruminating animal being with fistula, and its measurement result affects by multiple extraneous factor, causes measurement result to make a variation larger.In vitro method refers to and gathers fresh cud chyme or rumen fluid, microorganism culturing is carried out in the device of simulation rumen condition, so also known as artificial rumen method, relative to intracorporal method, half intracorporal method, in vitro method have simple to operate, time saving and energy saving, envrionment conditions easily controls, the feature such as reproducible, further, it can measure a large amount of Feed Samples within a short period of time.This method is suitable for the routine analysis in laboratory, and this seems particularly important for the modern ruminant animal nutrition research that order becomes complicated.
At present, typical in vitro method mainly contains batch culture and continuous culture method.
Batch culture is by microbial inoculant thing and fermentation substrate is disposable adds in fermenting container, after certain hour is cultivated, terminate to cultivate within the set time, therefore belong to static fermentation again, this method is owing to can not realize being separated of substrate and product, cause rumen zymosis environment to change, be therefore not suitable for long-term in vitro simulation fermentation test.Batch culture mainly contains two-phase method, aerogenesis method and simple digestion method etc.But, what in-vitro cultivation method achieved substrate (feed) and damping fluid enters the continuous discharge with chyme (solid phase and liquid phase) continuously, thus more close in vivo rumen zymosis situation, compared to the advantage batch culture with simulation rumen environment more accurately.
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; change can be expected easily or replace, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should described be as the criterion with the protection domain of claim.
Claims (10)
1. be applied in the constant temperature overflow device in Rumen simulation research, it is characterized in that, described overflow device comprises overflowing liquid receiving tube and weir tank;
It is inner that described overflowing liquid receiving tube stretches into described weir tank, for receiving the overflowing liquid from fermentor tank;
The tank skin of described weir tank is bilayer structure, is divided into inner and outer wall;
Described weir tank comprises the cooling fluid entrance and cooling liquid outlet that are arranged at described weir tank outer wall;
Cooling fluid can be injected in described bilayer structure by described cooling fluid entrance and described cooling liquid outlet, and keep described cooling fluid to circulate.
2. overflow device according to claim 1, is characterized in that, described overflow device also comprises the sealing plug be sealed and matched with described weir tank;
Described overflowing liquid receiving tube and described sealing plug are sealed and matched, and enter described weir tank inner through described sealing plug.
3. overflow device according to claim 2, is characterized in that, described overflow device comprises liquid level switch, and it is inner that described liquid level switch enters described weir tank through described sealing plug.
4. overflow device according to claim 3, is characterized in that, the bottom of described weir tank is provided with overflow box outlet;
Described overflow box outlet runs through the inner and outer wall of described weir tank;
Described overflow box outlet is connected with pinch valve by conduit, and described conduit extends from described pinch valve.
5. overflow device according to claim 4, is characterized in that, described conduit is silica gel hose.
6. overflow device according to claim 4, is characterized in that,
When the liquid level of the overflowing liquid in described weir tank rises to predeterminated position, described liquid level switch conducting, trigger described pinch valve and open, the overflowing liquid of described weir tank inside is flowed out by described conduit.
7. overflow device according to claim 4, is characterized in that, the tank skin of described weir tank is provided with liquid phase thief hatch;
Described liquid phase thief hatch runs through the inner and outer wall of described weir tank, for gathering the overflowing liquid in described weir tank.
8. overflow device according to claim 1, it is characterized in that, described overflow device also comprises the cooling fluid producer being arranged at described overflow filling outside, and described cooling fluid producer to described cooling fluid entrance conveying cooling fluid, and retrieves the cooling fluid from described cooling fluid entrance.
9. overflow device according to claim 8, it is characterized in that, described cooling fluid producer comprises the low temperature water pump and constant temperature water bath that are interconnected, and described low temperature water pump is communicated with described cooling fluid entrance, and described constant temperature water bath is communicated with described cooling liquid outlet.
10. overflow device according to claim 1, is characterized in that, described bilayer structure is welded by transparent glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510325740.7A CN104845865B (en) | 2015-06-15 | 2015-06-15 | A kind of constant temperature overflow mechanism applied in Rumen simulation is studied |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510325740.7A CN104845865B (en) | 2015-06-15 | 2015-06-15 | A kind of constant temperature overflow mechanism applied in Rumen simulation is studied |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104845865A true CN104845865A (en) | 2015-08-19 |
| CN104845865B CN104845865B (en) | 2016-08-17 |
Family
ID=53845857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510325740.7A Active CN104845865B (en) | 2015-06-15 | 2015-06-15 | A kind of constant temperature overflow mechanism applied in Rumen simulation is studied |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104845865B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565673A (en) * | 2009-06-02 | 2009-10-28 | 扬州大学 | Intelligent bionic rumen fermenter |
| CN101701182A (en) * | 2009-12-01 | 2010-05-05 | 北京科润维德生物技术有限责任公司 | Microbe biogas adhesive membrane type anaerobic fermentation reactor and application thereof |
| CN102286359A (en) * | 2010-12-28 | 2011-12-21 | 中国农业科学院北京畜牧兽医研究所 | Solid-liquid-gas three phase flow-dividing rumen imitation continuous fermentation system and method |
| CN203807463U (en) * | 2014-01-16 | 2014-09-03 | 黄山市迎客松生物科技有限公司 | Cooling water recycling device for fermentation tank |
-
2015
- 2015-06-15 CN CN201510325740.7A patent/CN104845865B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565673A (en) * | 2009-06-02 | 2009-10-28 | 扬州大学 | Intelligent bionic rumen fermenter |
| CN101701182A (en) * | 2009-12-01 | 2010-05-05 | 北京科润维德生物技术有限责任公司 | Microbe biogas adhesive membrane type anaerobic fermentation reactor and application thereof |
| CN102286359A (en) * | 2010-12-28 | 2011-12-21 | 中国农业科学院北京畜牧兽医研究所 | Solid-liquid-gas three phase flow-dividing rumen imitation continuous fermentation system and method |
| CN203807463U (en) * | 2014-01-16 | 2014-09-03 | 黄山市迎客松生物科技有限公司 | Cooling water recycling device for fermentation tank |
Non-Patent Citations (1)
| Title |
|---|
| 夏兆刚 孟庆翔: "双外流连续培养系统模拟瘤胃消化", 《中国饲料》, no. 23, 8 December 2002 (2002-12-08), pages 27 - 28 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104845865B (en) | 2016-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Theodorou et al. | A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds | |
| CN102286359B (en) | Solid-liquid-gas three phase flow-dividing rumen imitation continuous fermentation system and method | |
| CN101565673B (en) | Intelligent bionic rumen fermenter | |
| CN106047691B (en) | A kind of 3D cell culture systems | |
| CN106520552B (en) | A kind of biological reactor for cell culture | |
| CN104450860A (en) | Pneumonia mycoplasma medium | |
| CN110437973A (en) | A kind of culture apparatus and cultural method of anaerobic bacteria | |
| CN105754852A (en) | Equipment and measuring method for simulating growth curves of microorganisms in high-temperature and high-pressure oil reservoirs | |
| CN204803290U (en) | Use constant temperature overflow arrangement in cud analog study | |
| CN104845865A (en) | Constant-temperature overflow device applied to rumen simulation research | |
| CN203807476U (en) | Fermentation tank | |
| CN109385381B (en) | A kind of Urogenital Mycoplasma biphasic culture | |
| CN209098683U (en) | A kind of Novel anaerobic fermenting experiment device | |
| CN207958369U (en) | A kind of microbial detection device | |
| CN208676286U (en) | A kind of novel beans sauce rapid fermenting device | |
| CN206547854U (en) | Silage fermentation device is used in experiment | |
| CN108728375A (en) | The in vitro culture and store method of cow rumen flora | |
| CN206472834U (en) | A kind of food fermentation device of intelligent temperature control | |
| CN205981349U (en) | Simple and easy device of marsh gas volume in survey gas collection bag | |
| CN208917192U (en) | A kind of photosynthetic bacteria bactogen | |
| KR101449144B1 (en) | Rumen Simulation Continuous Culture System | |
| CN212833692U (en) | Single-station multi-mode fermented wine brewing device | |
| CN204625616U (en) | Emulation rumen fermenter | |
| CN104515851B (en) | Hydrogenase or the method and apparatus of carbon monoxide dehydrogenase activity in a kind of directly mensuration anaerobe | |
| CN102410983B (en) | Sterile dynamic detecting method for tissue culture seedling nitrogen use ratio |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |