CN104844837A - Thamnaconus modestus fish skin nanoparticle composite edible membrane - Google Patents
Thamnaconus modestus fish skin nanoparticle composite edible membrane Download PDFInfo
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- CN104844837A CN104844837A CN201510261069.4A CN201510261069A CN104844837A CN 104844837 A CN104844837 A CN 104844837A CN 201510261069 A CN201510261069 A CN 201510261069A CN 104844837 A CN104844837 A CN 104844837A
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Abstract
The invention discloses a thamnaconus modestus fish skin nanoparticle composite edible membrane which comprises the following components in parts by weight: 33-40 parts of gelatin, 0.65-1.2 parts of porphyra haitanensis antioxidant, 6.5-1 part of plasticizer and 55-60 parts of chitosan microsphere. The technology comprises the steps of preparing thamnaconus modestus fish skin gelatin, preparing chitosan nanoparticle and preparing porphyra haitanensis antioxidant, then preparing the prepared gelatin, chitosan nanoparticle and porphyra haitanensis antioxidant into a solution, and preparing a film-forming solution by the addition of the plasticizer, finally pouring the prepared film-forming solution on a polyvinyl chloride crystal board for striking, drying and film uncovering to obtain the antioxidant gelatin-chitosan nanoparticle composite edible membrane. The invention takes the thamnaconus modestus fish skin as the main raw material, so that a new approach for high-value use of the thamnaconus modestus processing leftovers is established; the prepared antioxidant gelatin-chitosan nanoparticle composite edible membrane has remarkable antioxidant activity and seaweed flavor.
Description
Technical field
The invention belongs to Food Quality and Safety technical field, relate to functional edible film, particularly relate to a kind of black scraper Puffer fish-skin Nano microsphere compound edible film.
Background technology
In recent years, along with the raising of people's living standard and the enhancing of environmental consciousness, the environmental problem that the Plastic Packaging Materials utilizing Nonrenewable resources to prepare causes receives increasing concern, thus becomes study hotspot to utilize renewable resource to prepare degradable packaging material for food.In numerous recyclable organism materials, the microbial film being material with the albumen in various source (as soybean protein, whey-protein, casein, zein and wheat protein) research is in widespread attention, and animal gelatin more becomes study hotspot due to characteristics such as its wide material sources, film forming ability are strong and bio-compatibility is good.
At present, animal gelatin mainly utilizes the raw materials such as the skin of the livestock product such as pig, ox, tendon and skeleton to prepare.But the outburst of Mammals epidemic disease and the increase of zoonosis, particularly mad cow disease, foot and mouth disease etc. is popular on a large scale, and the security of the gelatin making livestock product originate more is queried.Countries in the world are all at the gelatin material that positive searching is safer.And aquatic products processing tankage are as higher in gelatine content in fish-skin, fish scale, fish-bone, and the extensive development of aquaculture makes aquatic products gelatin substitute Mammals gelatin becomes possibility.Secondly, aquatic products gelatin also can be applied to halal food, expands the use range of gelatin product.
Gelatin has remarkable advantage as edible film research material, but its shortcoming is also clearly, relative to Plastic Packaging Materials, the tensile strength of gelatin edible film low and soluble in water limit its development, therefore, searching appropriate method solves the research emphasis that its existing defects becomes current.Secondly, along with the development of wrapping material, people wish that packaging microbial film is outside the basic function possessing wrapping material, more function can be had, as anti-oxidant in tool, have antibacterial, nutrition to strengthen isoreactivity, thus improve the quality of packaging food, Shelf-life.
Black scraper Puffer popular name rubber fish, peeling fish, being under the jurisdiction of Tetraodontiformes, leather Molidae, is a kind of Common Fish of China.Black scraper Puffer skin is thicker and hard, and without edibleness, majority is thrown away, and causes waste and the environmental pollution of resource.And now there are some researches show, containing a large amount of gelatin in black scraper Puffer fish-skin, be the quality matetrial preparing gelatin.Through retrieval, the research preparing gelatine-chitosan Nano microsphere compound edible film with black scraper Puffer fish-skin has no report.
Based on this, applicant for raw material, by series of processes process, prepares a kind of method of anti-oxidant gelatine-chitosan Nano microsphere compound edible film with black scraper Puffer fish-skin.
Summary of the invention
The object of the present invention is to provide a kind of method utilizing black scraper Puffer fish-skin to prepare anti-oxidant gelatine-chitosan Nano microsphere compound edible film.
For achieving the above object, technical scheme of the present invention is:
Black scraper Puffer fish-skin Nano microsphere compound edible film, it is by weight containing 33 ~ 40 parts of gelatin: 0.65 ~ 1.2 part of laver polyphenoils: 6.5 ~ 1 parts of softening agent: 55 ~ 60 parts of chitosan microballs.
The innovative approach taked also comprises, and softening agent preferably adopts sorbyl alcohol.
Gelatin employing preparation method is: rubbed by black scraper Puffer fish-skin, joins in the NaOH solution of 1mol/L and stirs 5 ~ 8 h, be washed till neutrality with distilled water according to solid-liquid ratio 1 g:10 ~ 15 mL; Then join in the HCl of 0.3 ~ 0.5% according to solid-liquid ratio 1 g:15 ~ 20 mL, soaking at room temperature 2 ~ 3 days, every 12 h change water once; The fish-skin homogenate finally will processed, joins in distilled water according to solid-liquid ratio 1 g:10 ~ 12 mL, in 65 ~ 70 DEG C of dynamic extraction 10 ~ 12 h, then in centrifugal 15 ~ 20 min of 9 000g, gets supernatant liquor, and concentrated, freeze-drying, obtains the gelatin of black scraper Puffer fish-skin.
Laver polyphenoils employing preparation method is: after new fresh laver tissue mashing, add 75% ethanol supersound extraction 1.5 ~ 2 h under room temperature according to 1g:8 ~ 10 mL, filter, filter residue repeats aforesaid operations 2 times, united extraction liquid also with Rotary Evaporators except desolventizing, obtains solid extract; Solid extract is added to the water according to solid-liquid ratio 1g:2 ~ 3 mL, isopyknic petroleum ether extraction is added three times after suspendible, remaining aqueous solution adds equal-volume extraction into ethyl acetate three times again, extraction into ethyl acetate solution Rotary Evaporators is removed desolventizing, obtains laver polyphenoils.
Chitosan microball is prepared in the following manner: join in the glacial acetic acid solution of 1% by chitosan according to solid-liquid ratio 1g:80 ~ 100 mL, it is made to dissolve completely in stirred overnight at room temperature, chitosan solution is in 0.8 μm of membrane filtration removing insoluble impurities, then with vigorous stirring, than 1:2 ~ 3 the Tri sodium Phosphate aqueous solution of 0.4% dropwise to be joined in chitosan solution according to Tri sodium Phosphate and chitosan mass and react 45 ~ 60 min, in 9 000g, 4 DEG C of centrifugal 25 ~ 30 min, get precipitation, then according to solid-liquid ratio 1 g:80 ~ 100 mL, washing postprecipitation is added in distilled water, at 0 ~ 4 DEG C after ultrasonic 5 ~ 10 min, freeze-drying obtains chitosan nano microballoon.
Compared with prior art, the invention has the advantages that:
1, the present invention utilizes black scraper Puffer fish-skin to be raw material, both can significantly improve the added value of black scraper Puffer processing, can reduce again black scraper Puffer fish-skin to the pollution of environment.
2, the compound edible film that prepared by the present invention contains laver polyphenoils, can suppress by UV-induced food lipid peroxidation, simultaneously again with good seaweed flavor, can extend the shelf-lives of food, improves food quality; Enrich food variety simultaneously, there is good DEVELOPMENT PROSPECT.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment:
(1) preparation of black scraper Puffer fishskin gelatin:by green fin black scraper Puffer (
thamnaconus modestus) fish-skin rubbing, join in the NaOH solution of 1mol/L according to solid-liquid ratio 1 g:10 mL and stir 7 h, be washed till neutrality with distilled water; Then join in the HCl of 0.5% according to solid-liquid ratio 1 g:20 mL, soaking at room temperature 3 days, every 12 h change water once; The fish-skin homogenate finally will processed, joins in distilled water according to solid-liquid ratio 1 g:12 mL, in 65 DEG C of dynamic extraction 12 h, then in centrifugal 15 min of 9 000g, gets supernatant liquor, and concentrated, freeze-drying, obtains black scraper Puffer fishskin gelatin.
(2) preparation of chitosan nano microballoon:chitosan is joined in the glacial acetic acid solution of 1% according to solid-liquid ratio 1g:80 mL, it is made to dissolve completely in stirred overnight at room temperature, chitosan solution is in 0.8 μm of membrane filtration removing insoluble impurities, then with vigorous stirring, than 1:3 the Tri sodium Phosphate aqueous solution of 0.4% dropwise to be joined in chitosan solution according to Tri sodium Phosphate and chitosan mass and react 60 min, in 9 000g, 4 DEG C of centrifugal 25 min, get precipitation, then according to solid-liquid ratio 1 g:100 mL, washing postprecipitation is added in distilled water, at 4 DEG C after ultrasonic 10 min, freeze-drying obtains chitosan nano microballoon.
(3) preparation of laver polyphenoils: by fresh porphyra haitanensis (
porphyra haitanensis) after tissue mashing, add 75% ethanol supersound extraction 1.5h under room temperature according to 1g:10 mL, filter, filter residue repeats aforesaid operations 2 times, united extraction liquid also with Rotary Evaporators except desolventizing, obtain solid extract; Solid extract is added to the water according to solid-liquid ratio 1g:3 mL, isopyknic petroleum ether extraction is added three times after suspendible, remaining aqueous solution adds equal-volume extraction into ethyl acetate three times again, extraction into ethyl acetate solution Rotary Evaporators is removed desolventizing, obtains laver polyphenoils.
(4) preparation of gelatine-chitosan Nano microsphere compound edible film film-forming soln: according to solid-liquid ratio 1g:30 mL, gelatin prepared by step (1) is joined in distilled water, stir 90 min in 50 DEG C to dissolve completely to gelatin, then according to 2% of gelatin weight, laver polyphenoils prepared by step (3) is joined gelatin solution, according to 20 ~ 25% of gelatin weight, sorbyl alcohol is joined gelatin solution, stir 25 min in 50 DEG C; Chitosan nano microballoon step (2) prepared joins in distilled water according to solid-liquid ratio 1g:15 mL, and ultrasonic 10 min, obtain chitosan nano microsphere suspension; Under whipped state, joined by chitosan nano microsphere suspension in isopyknic gelatin solution, stir 60 min in 30 DEG C, deaeration, makes film-forming soln.
(5) preparation of composite reactive edible film:be poured on equably by the film-forming soln modulated on polyvinyl chloride crystal slab and carry out striking film forming, 26 DEG C of relative humidity, 45% time drying 3 days, namely demoulding obtained black scraper Puffer fish-skin anti-oxidant gelatine-chitosan Nano microsphere compound edible film.
Black scraper Puffer fish-skin Nano microsphere compound edible film prepared by the present invention contains marine alga polyphenoils, can suppress by UV-induced food lipid peroxidation, simultaneously again with good seaweed flavor, can extend the shelf-lives of food, improves food quality; Enrich food variety simultaneously, there is good DEVELOPMENT PROSPECT.
Finally, still need it is noted that what enumerate above is only a specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (5)
1. black scraper Puffer fish-skin Nano microsphere compound edible film, is characterized in that: by weight containing 33 ~ 40 parts of gelatin: 0.65 ~ 1.2 part of laver polyphenoils: 6.5 ~ 1 parts of softening agent: 55 ~ 60 parts of chitosan microballs.
2. black scraper Puffer fish-skin Nano microsphere compound edible film according to claim 1, is characterized in that: described softening agent preferably adopts sorbyl alcohol.
3. black scraper Puffer fish-skin Nano microsphere compound edible film according to claim 1, it is characterized in that: described gelatin employing preparation method is: rubbed by black scraper Puffer fish-skin, join in the NaOH solution of 1mol/L according to solid-liquid ratio 1 g:10 ~ 15 mL and stir 5 ~ 8 h, be washed till neutrality with distilled water; Then join in the HCl of 0.3 ~ 0.5% according to solid-liquid ratio 1 g:15 ~ 20 mL, soaking at room temperature 2 ~ 3 days, every 12 h change water once; The fish-skin homogenate finally will processed, joins in distilled water according to solid-liquid ratio 1 g:10 ~ 12 mL, in 65 ~ 70 DEG C of dynamic extraction 10 ~ 12 h, then in centrifugal 15 ~ 20 min of 9 000g, gets supernatant liquor, and concentrated, freeze-drying, obtains the gelatin of black scraper Puffer fish-skin.
4. black scraper Puffer fish-skin Nano microsphere compound edible film according to claim 1, it is characterized in that: described laver polyphenoils employing preparation method is: after new fresh laver tissue mashing, 75% ethanol supersound extraction 1.5 ~ 2 h under room temperature is added according to 1g:8 ~ 10 mL, filter, filter residue repeats aforesaid operations 2 times, united extraction liquid also with Rotary Evaporators except desolventizing, obtains solid extract; Solid extract is added to the water according to solid-liquid ratio 1g:2 ~ 3 mL, isopyknic petroleum ether extraction is added three times after suspendible, remaining aqueous solution adds equal-volume extraction into ethyl acetate three times again, extraction into ethyl acetate solution Rotary Evaporators is removed desolventizing, obtains laver polyphenoils.
5. black scraper Puffer fish-skin Nano microsphere compound edible film according to claim 1, it is characterized in that: described chitosan microball is prepared in the following manner: chitosan is joined in the glacial acetic acid solution of 1% according to solid-liquid ratio 1g:80 ~ 100 mL, it is made to dissolve completely in stirred overnight at room temperature, chitosan solution is in 0.8 μm of membrane filtration removing insoluble impurities, then with vigorous stirring, than 1:2 ~ 3 the Tri sodium Phosphate aqueous solution of 0.4% dropwise to be joined in chitosan solution according to Tri sodium Phosphate and chitosan mass and react 45 ~ 60 min, in 9 000g, 4 DEG C of centrifugal 25 ~ 30 min, get precipitation, then according to solid-liquid ratio 1 g:80 ~ 100 mL, washing postprecipitation is added in distilled water, at 0 ~ 4 DEG C after ultrasonic 5 ~ 10 min, freeze-drying obtains chitosan nano microballoon.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036257A (en) * | 2016-08-12 | 2016-10-26 | 莆田市海发水产开发有限公司 | Formula feed for abalone culture and preparation method thereof |
| CN111647098A (en) * | 2020-06-17 | 2020-09-11 | 山东警察学院 | Fish skin-derived chondroitin sulfate/dermatan sulfate and application thereof |
-
2015
- 2015-05-21 CN CN201510261069.4A patent/CN104844837A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 《中国药用动物志》协作组: "《中国药用动物志 第二册》", 31 October 1983 * |
| 赵国玲 等: "坛紫菜不同溶剂组分的抗氧化活性", 《食品科学》 * |
| 鲍士宝: "茶多酚纳米粒复合明胶膜的研究", 《中国博士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036257A (en) * | 2016-08-12 | 2016-10-26 | 莆田市海发水产开发有限公司 | Formula feed for abalone culture and preparation method thereof |
| CN106036257B (en) * | 2016-08-12 | 2020-05-08 | 莆田市海发水产开发有限公司 | Abalone culture formula feed and preparation method thereof |
| CN111647098A (en) * | 2020-06-17 | 2020-09-11 | 山东警察学院 | Fish skin-derived chondroitin sulfate/dermatan sulfate and application thereof |
| CN111647098B (en) * | 2020-06-17 | 2022-05-17 | 山东警察学院 | Fish skin-derived chondroitin sulfate/dermatan sulfate and application thereof |
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Application publication date: 20150819 |