CN104830703B - One plant of rhodotorula mucilaginosa and its application in the red astaxanthin in fermenting and producing sea - Google Patents
One plant of rhodotorula mucilaginosa and its application in the red astaxanthin in fermenting and producing sea Download PDFInfo
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Abstract
This application discloses one plant of rhodotorula mucilaginosa (Rhodotorula mucilaginosa) GH101, and the invention also discloses application of the rhodotorula mucilaginosa in the red astaxanthin in fermenting and producing sea, realized by following steps：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Seed liquor is inoculated into containing in the red astaxanthin fermentation culture medium in sea according to the inoculum concentration of percent by volume 2 10%, in 25 33 DEG C, 7.0,120 160rpm shaken cultivations of pH5.5,60 76h, obtains the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea.Rhodotorula mucilaginosa provided by the invention can be used for the red astaxanthin in fermenting and producing sea, the red astaxanthin in sea is produced using rhodotorula mucilaginosa, and repeated screening is carried out to production bacterial strain, it obtains that one plant of growth and breeding power is strong, the red higher bacterial strain of astaxanthin yield in sea, good application prospect is provided with to the industrialized production of astaxanthin.
Description
Technical field
The present invention relates to microorganisms technical fields, and in particular to one plant of rhodotorula mucilaginosa and its in the red astaxanthin in fermenting and producing sea
In application.
Background technology
The red astaxanthin in sea is a Carotenoids, has extremely strong oxidation resistance and biological colorability, answers extensively
For many fields such as food, medicine, cosmetics, feed.Preventing cardiovascular and cerebrovascular disease, protect eyes and central nervous system
System, ultraviolet radiation preventing, inhibition tumour, anti-inflammatory, alleviation sports fatigue etc. have higher application value.Red shrimp is green in sea
Element, a kind of carotenoid with high anti-oxidation ability, its mobility and natural astaxanthin ester are close when thin-layer chromatography, antioxygen
Change ability is also close with natural left-handed astaxanthin and gains the name, and DPPH methods detect its oxidation resistance and are better than natural left-handed astaxanthin 20
~40% or so.
The preparation method of astaxanthin, it is known to chemical synthesis, method, the profit extracted from the extraction of aquatic products waste or algae
The method etc. produced with microorganism.Chemical synthesis, production cost is higher, and many countries do not allow to use synthesizing astaxanthin
For in terms of feed addictive；It is extracted from aquatic products waste, raw material sources are extensive, at low cost, but production is easily subject to seasonal restrictions, and produce
Condition requires harshness, and production cost is high, and yield is relatively low, and product purity is not high；It is extracted from algae, the content astaxanthin in algae
Higher, cost of material is relatively low.But the growth conditions of algae is harsh, and the requirement to water quality, environment and light is very high, large-scale production according to
So there are difficulty；The method produced using microorganism is conducive to large scale and high density and is given birth to using saccharine material fermenting and producing
Production, and do not limited by region and weather, production cost relative moderate.
In recent years, increasing to the demand of astaxanthin and similar products both at home and abroad, have a vast market foreground.Mesh
Many biotech companies of the states such as the preceding U.S., Canada, European Union are just being dedicated to development and production this kind of product, but far from satisfaction
The market demand, therefore higher price.Therefore step up to have the development of the red astaxanthin product in sea for enhancing our international competitiveness
Highly important meaning.
Invention content
In order to solve the above technical problems, the present invention provides one plant of rhodotorula mucilaginosa (Rhodotorula mucilaginosa)
GH101, deposit number are：CGMCC No.10123.
The present invention also provides application of the rhodotorula mucilaginosa in the red astaxanthin in fermenting and producing sea.
Application of the rhodotorula mucilaginosa in the red astaxanthin in fermenting and producing sea, is realized by following steps：By the glue
Rhodotorula successively carries out inclined-plane culture and seed culture, obtains activated seed liquid；According to the inoculum concentration of percent by volume 2-10%
Seed liquor is inoculated into and is contained in the red astaxanthin fermentation culture medium in sea, in 25-33 DEG C, pH5.5-7.0,120-160rpm shaken cultivation
60-76h, obtains the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, and the red astaxanthin fermentation medium component containing sea is：Grape
Sugared 6-48g/L, yeast powder 1-8g/L, urea 0.4-2g/L, potassium dihydrogen phosphate 0.1-0.8g/L, dipotassium hydrogen phosphate 0.1-0.8g/
L, sodium chloride 1-3g/L, remaining ingredient are water, pH5.5-6.8.
Preferably, 25-33 DEG C of the inclined-plane culture temperature, incubation time 60-76h, inclined-plane culture based component are：Malt
Powder 130.1g/L, distilled water 1000g/L, agar 15-20g/L are soaked, pH5.5-7.0 is adjusted.
Preferably, 25-30 DEG C of the seed culture temperature, incubation time 24-36h, 140~160r/min of shaking speed,
Seed culture based component is：Glucose 10-12g/L, yeast powder 2-3g/L, sodium chloride 6-9g/L, remaining ingredient are water, pH5.5-
7.0。
The Rhodotorula mucilaginose (English name Rhodotorula mucilaginosa) of the present invention, is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation day
Phase：On December 01st, 2014, deposit number：CGMCC No.10123.
The 18S rRNA gene orders of separated bacterial strain of the invention are sequenced, by the 18SrRNA gene sequences of acquisition
All sequences in database are compared analysis by row input GenBank by Blastn programs, as a result, it has been found that the present invention
The existing type strain that 18S rRNA gene orders and rhodotorula mucilaginosa belong to has higher similitude, such as table 1, wherein with
The sequence similarity of Rhodotorula mucilaginosa isolate ZH9 be 100%, and with the sequence of more plants of rhodotorula mucilaginosas
Row similitude is 98%, so that it is determined that the bacterial strain GH101 separated is the new strains system that rhodotorula mucilaginosa belongs to, and it is red to be named as glue
Yeast (Rhodotorula mucilaginosa) GH101.
18S rRNA amplifications are carried out, sequencing sequence is：
CATCCGTATTGTTCAAATTTTAAGCATAACAGTGAAACTGCGAATGGCTCATTAAATCAGTCATAGTTTATTTGATG
GTACCTTACTACATGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGAAAAATCCCGACTTCTGGAAGGGATGT
ATTTATTAGATCCAAAACCAATGGCCTTCGGGTCCCTATGGTGAATCATGATAACTGCTCGAATCGCATGGCCTTGC
GCCGGCGATGCTTCATTCAAATATCTGCCCTATCAACTTTCGATGGTAGGATAGAGGCCTACCATGGTGATGACGGG
TAACGGGGAATAAGGGTTCGATTCCGGAGAGAGGGCCTGAGAAACGGCCCTCAGGTCTAAGGACACGCAGCAGGCGC
GCAAATTATCCCCTGGCAACACTTTGCCGAGATAGTGACAATAAATAACAATGCAGGGCTCTTACGGGTCTTGCAAT
TGGAATGAGTACAATTTAAATCCCTTAACGAGGATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCC
AGCTCCAATAGCGTATATTAAAATTGTTGCCGTTAAAAAGCTCGTAGTCGAACTTCGGGCTCTGTCAGCCGGTCCGC
CTTCTTGGTGTGTACTTGTTTGACGGAGCCTTACCTCCTGGTGAACGGCGATGTCCTTTACTGGGTGTCGTCGCAAA
CCAGGACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCTTTGCCCGAATACATTAGCATGGAATAATAAAA
TAGGACGCGCGTTCCCATTTTGTTGGTTTCTGAGATCGCCGTAATGATTAATAGGGATAGTTGGGGGCATTTGTATT
CCGTCGTCAGAGGTGAAATTCTTGGATTGCCGGAAGACAAACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTG
ATCAAGAACGAAGGAAGGGGGATCGAAAACGATTAGATACCGTTGTAGTCTCTTCTGTAAACTATGCCAATTGGGGA
TCGGTACAGGATTTTTAATGACTGTATCGGCACCCGAAGAGAAATCTTTAAATGAGGTTCGGGGGGGAGTATGGTCG
CAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGTGTGGAGCCTGCGGCTTAATTTGACTCAACACGG
GGAAACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGATAGCTCTTTCTTGATCTTGTGGTTGGTGGTGCAT
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGATAACGAACGAG.By Blast sequence alignments.
1 gene order of table compares
Rhodotorula mucilaginosa provided by the invention can be used for the red astaxanthin in fermenting and producing sea.The present invention produces sea using rhodotorula mucilaginosa
Red astaxanthin, and carry out repeated screening to production bacterial strain, obtains that one plant of growth and breeding power is strong, the red higher bacterium of astaxanthin yield in sea
Strain, good application prospect and economic benefit are provided with to the industrialized production of astaxanthin.
The bacterial strain can generate the higher red astaxanthin in sea in pH5.5-7.2, but the red astaxanthin range in most high yield sea
Between pH6.5-7.0, between temperature is 25-30 DEG C, the red astaxanthin highest in the seas fermentation 72h, the red astaxanthin in sea is intracellular product,
Highest can account for the 1.16% of dry cell weight.
Description of the drawings
Attached drawing described herein is used to provide further understanding of the present invention, and constitutes the part of the present invention, this hair
Bright illustrative embodiments and their description are not constituted improper limitations of the present invention for explaining the present invention.In the accompanying drawings：
Fig. 1 is the flow chart using the red astaxanthin in rhodotorula mucilaginosa fermenting and producing sea of the present invention.
Specific implementation mode
Some vocabulary has such as been used to censure specific components in specification and claim.Those skilled in the art answer
It is understood that hardware manufacturer may call the same component with different nouns.This specification and claims are not with name
The difference of title is used as the mode for distinguishing component, but is used as the criterion of differentiation with the difference of component functionally.Such as logical
The "comprising" of piece specification and claim mentioned in is an open language, therefore should be construed to " include but do not limit
In "." substantially " refer in receivable error range, those skilled in the art can be described within a certain error range solution
Technical problem basically reaches the technique effect.In addition, " coupling " word includes any direct and indirect electric property coupling herein
Means.Therefore, if it is described herein that a first device is coupled to a second device, then representing the first device can directly electrical coupling
It is connected to the second device, or the second device indirectly electrically coupled through other devices or coupling means.Specification
Subsequent descriptions be implement the present invention better embodiment, so it is described description be by illustrate the present invention rule for the purpose of,
It is not limited to the scope of the present invention.Protection scope of the present invention is when subject to appended claims institute defender.
Below in conjunction with attached drawing, invention is further described in detail, but not as a limitation of the invention.
Embodiment 1：
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Wherein, described
25 DEG C, incubation time 60h of inclined-plane culture temperature, inclined-plane culture based component are：Fructus hordei germinatus leaching powder 130.1g/L, distilled water 1000g/L,
Agar 15g/L adjusts pH5.5.25 DEG C of the seed culture temperature, incubation time h for 24 hours, shaking speed 140r/min, seed
Medium component is：Glucose 10g/L, yeast powder 2g/L, sodium chloride 6g/L, remaining ingredient are water, pH5.5.
Seed liquor is inoculated into according to the inoculum concentration of percent by volume 2% and is contained in the red astaxanthin fermentation culture medium in sea, in 25
DEG C, pH5.5,120rpm shaken cultivation 60h obtain the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, described containing the red astaxanthin in sea
Fermentation medium components are：Glucose 6g/L, yeast powder 1g/L, urea 0.4g/L, potassium dihydrogen phosphate 0.1g/L, dipotassium hydrogen phosphate
0.1g/L, sodium chloride 1g/L, remaining ingredient are water, pH5.5.
Embodiment 2：
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Wherein, described
33 DEG C, incubation time 76h of inclined-plane culture temperature, inclined-plane culture based component are：Fructus hordei germinatus leaching powder 130.1g/L, distilled water 1000g/L,
Agar 20g/L adjusts pH7.0.30 DEG C, incubation time 36h, shaking speed 160r/min of the seed culture temperature, seed training
Foster based component is：Glucose 12g/L, yeast powder 3g/L, sodium chloride 9g/L, remaining ingredient are water, pH7.0.
Seed liquor is inoculated into according to the inoculum concentration of percent by volume 10% and is contained in the red astaxanthin fermentation culture medium in sea, in 33
DEG C, pH7.0,160rpm shaken cultivation 76h obtain the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, described containing the red astaxanthin in sea
Fermentation medium components are：Glucose g/L, yeast powder 8g/L, urea 2g/L, potassium dihydrogen phosphate 0.8g/L, dipotassium hydrogen phosphate
0.8g/L, sodium chloride 3g/L, remaining ingredient are water, pH6.8.
Embodiment 3：
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Wherein, described
26 DEG C, incubation time 65h of inclined-plane culture temperature, inclined-plane culture based component are：Fructus hordei germinatus leaching powder 130.1g/L, distilled water 1000g/L,
Agar 16g/L adjusts pH5.8.26 DEG C, incubation time 28h, shaking speed 150r/min of the seed culture temperature, seed training
Foster based component is：Glucose 10.5g/L, yeast powder 2.3g/L, sodium chloride 7g/L, remaining ingredient are water, pH5.8.
Seed liquor is inoculated into according to the inoculum concentration of percent by volume 3% and is contained in the red astaxanthin fermentation culture medium in sea, in 28
DEG C, pH6.0,130rpm shaken cultivation 65h obtain the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, described containing the red astaxanthin in sea
Fermentation medium components are：Glucose 15g/L, yeast powder 2g/L, urea 1.0g/L, potassium dihydrogen phosphate 0.4g/L, phosphoric acid hydrogen two
Potassium 0.4g/L, sodium chloride 2g/L, remaining ingredient are water, pH6.0.
Embodiment 4：
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Wherein, described
28 DEG C, incubation time 71h of inclined-plane culture temperature, inclined-plane culture based component are：Fructus hordei germinatus leaching powder 130.1g/L, distilled water 1000g/L,
Agar 17g/L adjusts pH5.9.28 DEG C, incubation time 28h, shaking speed 160r/min of the seed culture temperature, seed training
Foster based component is：Glucose 11g/L, yeast powder 2.5g/L, sodium chloride 8g/L, remaining ingredient are water, pH5.9.
Seed liquor is inoculated into according to the inoculum concentration of percent by volume 4% and is contained in the red astaxanthin fermentation culture medium in sea, in 29
DEG C, pH6.0,140rpm shaken cultivation 66h obtain the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, described containing the red astaxanthin in sea
Fermentation medium components are：Glucose 31g/L, yeast powder 3g/L, urea 1.2g/L, potassium dihydrogen phosphate 0.5g/L, phosphoric acid hydrogen two
Potassium 0.5g/L, sodium chloride 2.5g/L, remaining ingredient are water, pH6.1.
Embodiment 5：
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Wherein, described
29 DEG C, incubation time 72h of inclined-plane culture temperature, inclined-plane culture based component are：Fructus hordei germinatus leaching powder 130.1g/L, distilled water 1000g/L,
Agar 18g/L adjusts pH6.0.29 DEG C, incubation time 29h, shaking speed 160r/min of the seed culture temperature, seed training
Foster based component is：Glucose 10.6g/L, yeast powder 2.7g/L, sodium chloride 9g/L, remaining ingredient are water, pH6.0.
Seed liquor is inoculated into according to the inoculum concentration of percent by volume 5% and is contained in the red astaxanthin fermentation culture medium in sea, in 30
DEG C, pH6.2,150rpm shaken cultivation 71h obtain the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, described containing the red astaxanthin in sea
Fermentation medium components are：Glucose 37g/L, yeast powder 4g/L, urea 1.7g/L, potassium dihydrogen phosphate 0.6g/L, phosphoric acid hydrogen two
Potassium 0.6g/L, sodium chloride 1.7g/L, remaining ingredient are water, pH6.3.
Embodiment 6：
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Wherein, described
30 DEG C, incubation time 74h of inclined-plane culture temperature, inclined-plane culture based component are：Fructus hordei germinatus leaching powder 130.1g/L, distilled water 1000g/L,
Agar 18g/L adjusts pH5.9.29 DEG C, incubation time 36h, shaking speed 160r/min of the seed culture temperature, seed training
Foster based component is：Glucose 11g/L, yeast powder 2g/L, sodium chloride 6g/L, remaining ingredient are water, pH5.9.
Seed liquor is inoculated into according to the inoculum concentration of percent by volume 6% and is contained in the red astaxanthin fermentation culture medium in sea, in 30
DEG C, pH6.8,155rpm shaken cultivation 74h obtain the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, described containing the red astaxanthin in sea
Fermentation medium components are：Glucose 45g/L, yeast powder 7g/L, urea 1.8g/L, potassium dihydrogen phosphate 0.6g/L, phosphoric acid hydrogen two
Potassium 0.6g/L, sodium chloride 1.9g/L, remaining ingredient are water, pH6.8.
Embodiment 7
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Wherein, described
31 DEG C, incubation time 73h of inclined-plane culture temperature, inclined-plane culture based component are：Fructus hordei germinatus leaching powder 130.1g/L, distilled water 1000g/L,
Agar 19g/L adjusts pH6.0.30 DEG C, incubation time 24-36h, shaking speed 160r/min of the seed culture temperature, seed
Medium component is：Glucose 11g/L, yeast powder 2g/L, sodium chloride 8g/L, remaining ingredient are water, pH6.0.
Seed liquor is inoculated into according to the inoculum concentration of percent by volume 7% and is contained in the red astaxanthin fermentation culture medium in sea, in 31
DEG C, pH6.9,160rpm shaken cultivation 75h obtain the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, described containing the red astaxanthin in sea
Fermentation medium components are：Glucose 38g/L, yeast powder 8g/L, urea 1.6g/L, potassium dihydrogen phosphate 0.7g/L, phosphoric acid hydrogen two
Potassium 0.7g/L, sodium chloride 1.7g/L, remaining ingredient are water, pH6.5.
Embodiment 8
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Wherein, described
32 DEG C, incubation time 70h of inclined-plane culture temperature, inclined-plane culture based component are：Fructus hordei germinatus leaching powder 130.1g/L, distilled water 1000g/L,
Agar 17g/L adjusts pH6.1.28 DEG C, incubation time 36h, shaking speed 160r/min of the seed culture temperature, seed training
Foster based component is：Glucose 11g/L, yeast powder 2.5g/L, sodium chloride 7.5g/L, remaining ingredient are water, pH6.1.
Seed liquor is inoculated into according to the inoculum concentration of percent by volume 8% and is contained in the red astaxanthin fermentation culture medium in sea, in 25-
33 DEG C, pH6.1,150rpm shaken cultivation 64h, obtain the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, and the red shrimp containing sea is green
Plain fermentation medium components are：Glucose 40g/L, yeast powder 3g/L, urea 1.5g/L, potassium dihydrogen phosphate 0.7g/L, phosphoric acid hydrogen
Dipotassium 0.7g/L, sodium chloride 1.9g/L, remaining ingredient are water, pH6.1.
Embodiment 9
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；Wherein, described
30 DEG C, incubation time 76h of inclined-plane culture temperature, inclined-plane culture based component are：Fructus hordei germinatus leaching powder 130.1g/L, distilled water 1000g/L,
Agar 19g/L adjusts pH6.0.30 DEG C, incubation time 24-36h, shaking speed 160r/min of the seed culture temperature, seed
Medium component is：Glucose 12g/L, yeast powder 2.4g/L, sodium chloride 7g/L, remaining ingredient are water, pH6.0.
Seed liquor is inoculated into according to the inoculum concentration of percent by volume 9% and is contained in the red astaxanthin fermentation culture medium in sea, in 30
DEG C, pH6.0,160rpm shaken cultivation 68h obtain the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, described containing the red astaxanthin in sea
Fermentation medium components are：Glucose 45g/L, yeast powder 7g/L, urea 0.9g/L, potassium dihydrogen phosphate 0.7g/L, phosphoric acid hydrogen two
Potassium 0.7g/L, sodium chloride 2.8g/L, remaining ingredient are water, pH6.0.
Embodiment 10
The present embodiment provides application of the rhodotorula mucilaginosa in the present invention in the red astaxanthin in fermenting and producing sea, pass through following step
It is rapid to realize：The rhodotorula mucilaginosa is successively subjected to inclined-plane culture and seed culture, obtains activated seed liquid；According to volume ratio
Seed liquor is inoculated into the fermentation medium of the red astaxanthin in production sea by 3% inoculum concentration, in 25-30 DEG C, pH5.5-7.0,
For 160rpm shaken cultivations 72h to get to the zymotic fluid of the rhodotorula mucilaginosa thalline containing astaxanthin, wherein carbon source is glucose, institute
The production astaxanthin fermentation medium component stated is：Glucose 30g/L, yeast powder 5g/L, urea 2g/L, potassium dihydrogen phosphate 0.5g/
L, dipotassium hydrogen phosphate 0.5g/L, sodium chloride 3g/L, remaining ingredient are water, pH6.5.
The bacterial strain can generate higher astaxanthin in pH5.5-7.2, but most high-yield astaxanthin range is in pH6.5-
Between 7.0, between temperature is 25-30 DEG C, the red astaxanthin highest in the seas fermentation 72h, astaxanthin is intracellular product, and highest can account for thalline
The 1.16% of dry weight.
Several preferred embodiments of the present invention have shown and described in above description, but as previously described, it should be understood that the present invention
Be not limited to form disclosed herein, be not to be taken as excluding other embodiments, and can be used for various other combinations,
Modification and environment, and the above teachings or related fields of technology or knowledge can be passed through in the scope of the invention is set forth herein
It is modified.And changes and modifications made by those skilled in the art do not depart from the spirit and scope of the present invention, then it all should be in this hair
In the protection domain of bright appended claims.
Claims (2)
1. a kind of application of rhodotorula mucilaginosa in the red astaxanthin in fermenting and producing sea, which is characterized in that the rhodotorula mucilaginosa
(Rhodotorula mucilaginosa) GH101, deposit number are：CGMCC No.10123, are realized by following steps：It will
The rhodotorula mucilaginosa successively carries out inclined-plane culture and seed culture, obtains activated seed liquid, the seed culture temperature 25-30
DEG C, incubation time 24-36h, shaking speed 140-160r/min, seed culture based component is：Glucose 10-12g/L, yeast powder
2-3g/L, sodium chloride 6-9g/L, remaining ingredient are water, pH5.5-7.0；According to the inoculum concentration of percent by volume 2-10% by seed
Liquid, which is inoculated into, to be contained in the red astaxanthin fermentation culture medium in sea, in 25-33 DEG C, pH5.5-7.0,120-160rpm shaken cultivation 60-
76h, obtains the rhodotorula mucilaginosa thalline containing the red astaxanthin in sea, and the red astaxanthin fermentation medium component containing sea is：Glucose
6-48g/L, yeast powder 1-8g/L, urea 0.4-2g/L, potassium dihydrogen phosphate 0.1-0.8g/L, dipotassium hydrogen phosphate 0.1-0.8g/L,
Sodium chloride 1-3g/L, remaining ingredient are water, pH5.5-6.8.
2. application of the rhodotorula mucilaginosa according to claim 1 in the red astaxanthin in fermenting and producing sea, which is characterized in that described
25-33 DEG C of inclined-plane culture temperature, incubation time 60-76h, inclined-plane culture based component is：Fructus hordei germinatus leaching powder 130.1g/L, distilled water
1000g/L, agar 15-20g/L adjust pH5.5-7.0.
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| CN101706193A (en) * | 2009-08-06 | 2010-05-12 | 李观德 | Drying method and drying device for charcoal making |
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