CN104826482A - Gas-liquid two-phase bacterial culture method for waste gas biotreatment - Google Patents
Gas-liquid two-phase bacterial culture method for waste gas biotreatment Download PDFInfo
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Abstract
The invention relates to a gas-liquid two-phase bacterial culture method for waste gas biotreatment. The method is characterized in that it uses a gas-liquid two-phase bacterial culture device special for waste gas biotreatment, and by means of the device, strains and a culture solution can enter a bacterial culture tank (1) from an inocula conveying system (15) and a liquid material storage tank (18). The device starts quickly when used for follow-up bioreactors, and is efficient and stable in pollutant removal.
Description
Technical field
Patent of the present invention relates to a kind of method of the gas-liquid two-phase cultivation for waste bio-treatment.
Background technology
The biological treatment process of waste gas, essence is attached to microorganism on biologic packing material medium under adapt circumstance condition, utilizes pollutant in waste gas as carbon source and the energy, maintain its vital movement, pollutant is decomposed into CO simultaneously
2, H
2the process of the Small molecular inorganic matters such as O is a novel environment friendly Treatment process; Simultaneously because this technical matters is simple, easy and simple to handle, cost is low, is day by day subject to people's attention in waste gas pollution control and treatment field.
Biological treatment device for exhaust gas startup stage, all need microbe inoculation, particularly process difficult for biological degradation, be difficult to laboratory simulation obtain waste gas time, more need to screening and separating obtained degraded bacterial classification carry out in-situ batch culture amplification.The cultivation of existing waste bio-treatment industrial microorganism, directly carry out in bioreactor, this makes start-up time relatively long, but in laboratory screening and separating obtained degradation bacteria, single culture often, and on-the-spot waste gas composition multiple blending ingredients and in change often, therefore be difficult to the technological requirement being applicable to industrial waste gas biological process.Therefore, though in this area urgently research and development also to turn out the bacteria culture apparatus and method that are applicable to industrial mix waste gas in laboratory.
Summary of the invention
The object of patent of the present invention is a kind of method providing gas-liquid two-phase cultivation for waste bio-treatment.For reaching goal of the invention, the technical solution used in the present invention is as follows:
A kind of method of the gas-liquid two-phase cultivation for waste bio-treatment, it is characterized in that, the method adopts a kind of gas-liquid two-phase cultivation special purpose device for waste bio-treatment, by this device, bacterial classification and nutrient solution are entered in cultivation tank (1) by cultivation liquid induction system (15) and liquid storage tank (18), waste gas enters cultivation tank (1) by blower fan (2) by blending tank (8), be dispersed into micro-bubble through micropore gas pipe (12) to contact with nutrient solution with bacterial classification, cultivation temperature is monitored by temp probe (10), the pH of cultivation system is monitored by pH probe (11), cultivate after 3 ~ 20 days, mixed bacteria liquid is by cultivation liquid induction system (15) target approach biological treatment system, on the biologic packing material medium of sprinkle in biological treatment system, and start the biological treatment of waste gas.
The described gas-liquid two-phase cultivation special purpose device for waste bio-treatment, is characterized in that comprising with lower device:
Cultivation tank (1), waste gas is through simulated exhaust air distributing device, electrical control cubicles (9), micropore gas pipe (12), heater (13), shelf (14), cultivation liquid induction system (15), nutrient tank (17), with the liquid storage tank (18) of Target exhaust same nature, device for absorbing tail gas (19);
Described cultivation tank (1) inside has shelf (14), bottom has micropore gas pipe (12), the bottom of this micropore gas pipe (12) has heater (13), this heater (13) is connected with electrical control cubicles (9), bottom side-walls and the cultivation liquid induction system (15) of described cultivation tank (1) communicate, the top of this cultivation tank (1) and nutrient tank (17), be connected with the liquid storage tank (18) of Target exhaust same nature and device for absorbing tail gas (19)
Described waste gas comprises blower fan (2) through simulated exhaust air distributing device, volatile fluid contaminants storage tank (4), Target exhaust steel cylinder (7), blending tank (8), the side of described blending tank (8) bottom is connected with one end of volatile fluid contaminants storage tank (4), bottom opposite side is connected with Target exhaust steel cylinder (7), the other end of volatile fluid contaminants storage tank (4) is connected with blower fan (2), and described blending tank (8) is connected with micropore gas pipe (12).
The inside of described cultivation tank (1) also has temp probe (10) and pH probe (11), and this temp probe (10) is connected with electrical control cubicles (9) with pH probe (11).
Between described volatile fluid contaminants storage tank (4) and blower fan (2) and between blending tank (8) and micropore gas pipe (12), all there is flowmeter (3).
There is between described Target exhaust steel cylinder (7) and blending tank (8) mass flowmenter (5) and check-valves (6).
The quantity of described shelf (14) is two.
Described nutrient tank (17) and and between the liquid storage tank (18) of Target exhaust same nature and cultivation tank (1), there is measuring pump (16).
The mixture of the liquid composition formed using simulated target waste gas or liquid state and gaseous component is as nutrient source, domestication cultivation is carried out 3 ~ 20 days to bacterial classification, on the biologic packing material of the direct sprinkle of mixed bacteria liquid that cultivation obtains in bioreactor, the bioreactor of the described Target exhaust of process is started fast.
When described Target exhaust contains H
2during the waste gas of S, with Na
2s solution substitutes the H in waste gas
2s, simulated exhaust composition is as nutrient source.When described Target exhaust contains the waste gas of methyl mercaptan, substitute the methyl mercaptan in waste gas with sodium methyl mercaptide solution, simulated exhaust composition is as nutrient source.
Described bacterial classification is one of following or wherein two or more mixture: the 1. single purifying bacterial classification in laboratory; 2. activated sludge or activated sludge supernatant; 3. the mixed bacteria liquid of similar waste gas bioreactor is processed; 4. the soil around exhaust emission or the extract of water.
Concrete, described method is as follows:
(1) hard objectives waste gas composition, injects bacterial classification and nutrient solution, the liquid composition simultaneously passing into simulated target waste gas composition or mixture that is liquid and gaseous component in cultivation tank;
(2) control pH value in cultivation tank, temperature in the scope that described bacterial classification is suitable, constantly add nutrient solution, domestication cultivation 3 ~ 20 days;
(3) cultured mixed bacteria liquid is delivered to bioreactor, circulation sprinkle, on biologic packing material, passes into waste gas simultaneously, bioreactor is started fast in bioreactor.
The device that a kind of described cultivation method is used, comprise cultivation tank, simulated exhaust air distribution system, electric control gear, liquid material liquid feeding system, nutrient solution liquid feeding system and exhaust gas purification system, described cultivation tank top is provided with bacterial classification entrance, filling opening, exhaust port, inside is provided with temp probe, pH probe, heater and micropore gas pipe, bottom is provided with exhaust gas entrance and the outlet of bacterium liquid, described exhaust gas entrance is by tracheae and described waste gas air distribution system conducting, and temp probe, pH probe, heater operate control by electric control gear.Described bacterium liquid outlet can be connected with bioreactor.
Be provided with dividing plate in described cultivation tank, biologic packing material placed by dividing plate.
Described simulated exhaust air distribution system is provided with mass flowmenter, check-valves and flowmeter.In the course of cultivating the microorganism of waste gas at the scene, can switch to waste gas collecting pipe, this collection tube can be communicated with on-the-spot waste gas airduct, and when oxygen content is not enough, described waste gas collecting pipe can be provided with air bypass passage.
Described cultivation tank inside is provided with DO, ORP probe, so that control reaction condition.
Described cultivation tank can make cross section for circular, square or other suitable shape, its material can select the synthetic material such as PVC, lucite and or the metal material such as steel plate, as contained H in waste gas
2the catabolites such as S are the pollutant of acidic materials, then containing sour sulfur bacillus in cultivated mixed bacteria liquid, device needs anticorrosion.
The cultivation tank of exhausted air quantity size that described bacteria culture apparatus processes as required design different size and accessory kit, when needed, can be transported at any time need applying biological method process waste gas Anywhere scene or laboratory in.
Of the present inventionly to be mainly reflected in for the gas-liquid two-phase cultivation method of waste bio-treatment and the advantage of device thereof: it is rapid that (1) described method and apparatus is applied to subsequent bio reactor start-up, pollutant removal efficient stable; (2) with the liquid of Target exhaust or same nature or both mixtures as microorganism culture substrate (matrix), in the mixed bacteria liquid cultivated, microbe species enriches, and with strong points, adaptability is good; (3) directly also directly contact with nutrient solution as substrate with the liquid state composition of similar properties, eliminate Spawn incubation process toxic and harmful (as H
2s, carrene, methyl mercaptan etc.) as difficulty operational during substrate cultivation and unsafe factor (poison gas leakage); (4) structure is simple, easy to use, can be made into mobile device, strong adaptability.
The benefit of method of the present invention to shorten the strain domestication time of biological treatment device for exhaust gas, and directly spray on the filler in biological treatment device for exhaust gas after cultivation completes, the same day is to H in waste gas
2the clearance of S can reach 85%, can reach more than 50% to the clearance of VOC in waste gas, and general biological treatment device for exhaust gas needs the domestication of 10 ~ 15 days just can reach this effect.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.
See Fig. 1, a kind of cultivation special purpose device of waste bio-treatment, mainly comprise cultivation tank (1), simulated exhaust air distribution system (2 ~ 8), electrical control cubicles (9), nutrient solution liquid feeding system (16, 17), liquid material liquid feeding system (16, 18) and exhaust gas purification system (19), described cultivation tank inside is provided with temp probe (10), pH pops one's head in (11), micropore gas pipe (12) and heater (13), bottom is provided with cultivation liquid induction system (15), except described waste gas air distribution system, the on-the-spot waste gas airduct of exhaust gas entrance Guan Keyu is communicated with, described exhaust gas entrance is connected with micropore gas pipe (12).
During actual use, bacterial classification and nutrient solution are by nutrient solution liquid feeding system (15, 16) cultivation tank is entered, waste gas is through simulated exhaust air distribution system (2 ~ 8) or on-the-spot waste gas airduct, cultivation tank (1) is entered by exhaust gas entrance, be dispersed into micro-bubble through micropore gas pipe (12) to contact with nutrient solution with bacterial classification, cultivation temperature is monitored by temp probe (10), the pH of cultivation system is monitored by pH probe (11), cultivate after 3 ~ 20 days, mixed bacteria liquid is by cultivation liquid induction system (15) target approach biological treatment system, on the biologic packing material medium of sprinkle in biological treatment system, start the biological treatment of waste gas.
Embodiment 1:
With biofilter PROCESS FOR TREATMENT Sewage Treatment at Pharmaceutical Factory mix waste gas, containing H in waste gas
2s concentration is 300 ~ 500mg/m
3, be 250 ~ 2000mg/m containing VOCs (being mainly toluene and carrene etc.) concentration
3, adopt this gas-liquid two-phase cultivation method and device thereof to have unique advantage.First mixed liquor is jointly formed as bacterial classification with single microorganism bacterial classification prepared by laboratory with the activated sludge of this Sewage Treatment at Pharmaceutical Factory factory aerobic biochemical pool, toluene, dichloromethane solution are placed in volatile fluid contaminants storage tank, open blower fan air and toluene, carrene are blown into blending tank, after mixing, pass into cultivation tank (1).In the lab, due to H
2s toxicity is comparatively large, H
2s exhaust gas source is more difficult acquisition often, adopts steel cylinder gas to there is again too many unsafe factor, therefore adopt Na in experimentation
2s solution instead of H
2s exhaust gas source, by Na
2s solution is placed in liquid storage tank (18), accurately adds material by measuring pump.Cultivate after 5 days at the scene, pH of mixed is down to 3.2 from 7.8, and then control the pH of cultivation tank between 3.5 ~ 4.0, then cultivate 7 days, the concentration of suspension in cultivation tank significantly increases, and rule of thumb can think that mixed bacteria liquid has been cultivated.
At the 13rd day, namely cultivated mixed bacteria liquid, sprinkle was on the filler of biological thing filter bed respectively, and in waste gas, pollutant removal result shows: the H in waste gas
2s clearance reached 85% the same day, within the 2nd day, reached 95%, the 3rd day more than 99%; In waste gas, the clearance of VOCs reached 50% the same day, within the 2nd day, reached 70%, within the 3rd day, reached more than 85%.The strain separating of cultivated mixed bacteria liquid and qualification result are shown, mainly contains Thiobacillus (Thiobacillus), bacillus (Bacillus), xanthomonas (Xanthomonas), pseudomonas (Pseudomonas), Actinomy cetaceae Mycobacterium (Mycobacterium) etc.
Embodiment 2:
With biofilter PROCESS FOR TREATMENT Zhejiang fish meal factory waste gas, mainly containing H
2s and methyl mercaptan etc., H
2the concentration of S is 50 ~ 120mg/m
3, organic sulfide substrate concentration is 30 ~ 80mg/m
3adopt this gas-liquid two-phase bacteria culture apparatus, culture substrate adopts sodium sulfide solution, with the process mixed bacteria liquid of similar waste gas bioreactor and activated sludge as bacterial classification, cultivate after 3 days, pH of mixed is down to 2.8 from 7.3, and the concentration of suspension in cultivation tank significantly increases, and rule of thumb can think that mixed bacteria liquid has been cultivated.
At the 4th day, namely cultivated mixed bacteria liquid, sprinkle was on the filler of biofilter respectively, and in waste gas, pollutant removal result shows: H in waste gas
2the clearance of S reached 85% the same day, within the 2nd day, reached more than 95%; The clearance of organic sulfur compound reached 82% the same day, within the 2nd day, reached more than 90%.The strain separating of cultivated mixed bacteria liquid and qualification result are shown, mainly contain Thiobacillus (Thiobacillus), mucor (Mucor), rhizopus (Rhizopus), aspergillus (Aspergillus) etc.
Embodiment 3:
With chemical plant, Biotrickling filter PROCESS FOR TREATMENT Zhejiang mix waste gas, mainly containing benzene homologues, H
2s and the organic sulfur compound such as methyl mercaptan, ethyl mercaptan waste gas, in waste gas, containing benezene compound concentration is 100 ~ 360mg/m
3, H
2s concentration is 180 ~ 450mg/m
3, organic sulfide substrate concentration is 35 ~ 120mg/m
3adopt this gas-liquid two-phase bacteria culture apparatus, culture substrate adopts benzene+toluene+vulcanized sodium, by enterprise's sludge sewage as bacterial classification, cultivate after 7 days, pH of mixed is down to 3.0 from 7.3, then the pH of cultivation tank is controlled between 3.5 ~ 4.0, after cultivating 7 days again, the concentration of suspension in cultivation tank significantly increases, and rule of thumb can think that mixed bacteria liquid has been cultivated.
At the 15th day, namely cultivated mixed bacteria liquid, sprinkle was on the filler of Biotrickling filter respectively, and in waste gas, pollutant removal result shows: H in waste gas
2the clearance of S reached 85% the same day, within the 2nd day, reached 93%, within the 3rd day, reached 99%; The clearance of benzene-like compounds reached 55% the same day, within the 3rd day, reached 70%, within the 5th day, reached more than 85%; The clearance of organic sulfur compound reached 80% the same day, within the 2nd day, reached 85%, within the 3rd day, reached more than 95%.The strain separating of cultivated mixed bacteria liquid and qualification result are shown, mainly contain aerobic bacillus (Bacillus), xanthomonas (Xanthomonas), pseudomonas (Pseudomonas), Thiobacillus (Thiobacillus), mucor (Mucor), Penicillium (Penicillium), rhizopus (Rhizopus), aspergillus (Aspergillus) etc.
Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Claims (7)
1. the method for the gas-liquid two-phase cultivation of waste bio-treatment, it is characterized in that, the method adopts a kind of gas-liquid two-phase cultivation special purpose device for waste bio-treatment, by this device, bacterial classification and nutrient solution are entered in cultivation tank (1) by cultivation liquid induction system (15) and liquid storage tank (18), waste gas enters cultivation tank (1) by blower fan (2) by blending tank (8), be dispersed into micro-bubble through micropore gas pipe (12) to contact with nutrient solution with bacterial classification, cultivation temperature is monitored by temp probe (10), the pH of cultivation system is monitored by pH probe (11), cultivate after 3 ~ 20 days, mixed bacteria liquid is by cultivation liquid induction system (15) target approach biological treatment system, on the biologic packing material medium of sprinkle in biological treatment system, and start the biological treatment of waste gas.
2. the method for a kind of gas-liquid two-phase cultivation for waste bio-treatment as claimed in claim 1, is characterized in that the described gas-liquid two-phase cultivation special purpose device for waste bio-treatment, it is characterized in that comprising with lower device:
Cultivation tank (1), waste gas is through simulated exhaust air distributing device, electrical control cubicles (9), micropore gas pipe (12), heater (13), shelf (14), cultivation liquid induction system (15), nutrient tank (17), with the liquid storage tank (18) of Target exhaust same nature, device for absorbing tail gas (19);
Described cultivation tank (1) inside has shelf (14), bottom has micropore gas pipe (12), the bottom of this micropore gas pipe (12) has heater (13), this heater (13) is connected with electrical control cubicles (9), bottom side-walls and the cultivation liquid induction system (15) of described cultivation tank (1) communicate, the top of this cultivation tank (1) and nutrient tank (17), be connected with the liquid storage tank (18) of Target exhaust same nature and device for absorbing tail gas (19)
Described waste gas comprises blower fan (2) through simulated exhaust air distributing device, volatile fluid contaminants storage tank (4), Target exhaust steel cylinder (7), blending tank (8), the side of described blending tank (8) bottom is connected with one end of volatile fluid contaminants storage tank (4), bottom opposite side is connected with Target exhaust steel cylinder (7), the other end of volatile fluid contaminants storage tank (4) is connected with blower fan (2), and described blending tank (8) is connected with micropore gas pipe (12).
3. the method for a kind of gas-liquid two-phase cultivation for waste bio-treatment as claimed in claim 2, it is characterized in that, the inside of described cultivation tank (1) also has temp probe (10) and pH probe (11), and this temp probe (10) is connected with electrical control cubicles (9) with pH probe (11).
4. the method for a kind of gas-liquid two-phase cultivation for waste bio-treatment as claimed in claim 2, it is characterized in that, between described volatile fluid contaminants storage tank (4) and blower fan (2) and between blending tank (8) and micropore gas pipe (12), all there is flowmeter (3).
5. a kind of gas-liquid two-phase cultivation special purpose device for waste bio-treatment as claimed in claim 2, it is characterized in that there is between described Target exhaust steel cylinder (7) and blending tank (8) mass flowmenter (5) and check-valves (6).
6. a kind of gas-liquid two-phase cultivation special purpose device for waste bio-treatment as claimed in claim 2, it is characterized in that, the quantity of described shelf (14) is two.
7. a kind of gas-liquid two-phase cultivation special purpose device for waste bio-treatment as claimed in claim 2, it is characterized in that, described nutrient tank (17) and and between the liquid storage tank (18) of Target exhaust same nature and cultivation tank (1), there is measuring pump (16).
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| CN106987517A (en) * | 2017-05-02 | 2017-07-28 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of VOCs degradation floras quickly tame the system and method for enrichment |
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