CN104805003A - Bacterium coating rod provided with temperature sensor - Google Patents

Bacterium coating rod provided with temperature sensor Download PDF

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Publication number
CN104805003A
CN104805003A CN201510238971.4A CN201510238971A CN104805003A CN 104805003 A CN104805003 A CN 104805003A CN 201510238971 A CN201510238971 A CN 201510238971A CN 104805003 A CN104805003 A CN 104805003A
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CN
China
Prior art keywords
temperature sensor
coating end
handle
bacterium
temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510238971.4A
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Chinese (zh)
Inventor
徐加英
秦立强
赵琳
段广新
童星
周如鸿
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Suzhou University
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Suzhou University
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Filing date
Publication date
Application filed by Suzhou University filed Critical Suzhou University
Priority to CN201510238971.4A priority Critical patent/CN104805003A/en
Publication of CN104805003A publication Critical patent/CN104805003A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a bacterium coating rod provided with a temperature sensor. The bacterium coating rod comprises a handle and a coating end which is connected with the handle, wherein the handle and the coating end are integrally formed; the coating end is in the shape of a equilateral triangle; the handle and the coating end are respectively provided with a hollow chamber; the temperature sensor is arranged in the hollow chamber of the coating end, and a temperature indicating light and a comparer circuit are arranged in the hollow chamber of the handle; the temperature sensor is electrically connected with the comparer circuit; the comparer circuit is electrically connected with the temperature indicating light. The bacterium coating rod disclosed by the invention has the advantages of being capable of preventing over-high temperature of the coating end so that the bacterium is not killed and the experimental results are not affected, and judging whether the coating end is at a safe temperature according to the indicating light, so the wait time is greatly shortened and the work efficiency is improved.

Description

A kind of bacterium spreading rod with temperature sensor
Technical field
The present invention relates to a kind of bacterium spreading rod with temperature sensor, belong to experiment apparatus technical field.
Background technology
In the experiment such as microbe to screen, antibiotics susceptibility test, need the liquid sample containing microorganism to coat solid culture planar surface.The bacterium spreading rod that existing market is sold is roughly divided into two kinds, and a class is disposable hand-held plastic-coated rod; Another kind of is glass or metal repeatedly used spreading rod.The two Problems existing is and is unfavorable for once carrying out a large amount of Bacterial Transformation experiments.Disposable plastics bacterium spreading rod quantitatively can meet requirement of experiment, but waste material, also easily cause pollution to environment owing to spreading rod speckling with bacterium in addition.Glass or the spreading rod of metal products are then difficult to once prepare dozens of spreading rod, Reusability after although metal bar can overdo, but this kind of spreading rod temperature fall time after overdoing is long, and operator are difficult to ensure that the temperature of spreading rod drops to less than 37 DEG C, if temperature drift, easily scald the strain bio of dead coating, thus affect experimental result, if cooling time is oversize, waste a large amount of waiting time; Glass spreading rod on the one hand can by soaking 75% alcohol to reuse, but each treatment time needs more than 20 minutes, Reusability after another aspect glass spreading rod also can overdo, but so just there is same problem with metal coated rod, in sum, there is all drawbacks in existing method, has had a strong impact on the accuracy rate of conventional efficient and experimental result.
Therefore, be badly in need of a kind of easy to use, the bacterium spreading rod of cooling degree can be judged intuitively, easily, solve problems of the prior art, ensure carrying out smoothly of microbial culture experiment, the accuracy of increasing work efficiency and testing.
Summary of the invention
Goal of the invention of the present invention is to provide a kind of bacterium spreading rod with temperature sensor.
To achieve the above object of the invention, the technical solution used in the present invention is: a kind of bacterium spreading rod with temperature sensor, the coating end comprising handle and be connected with handle, described handle and coating end are structure as a whole, described coating end is in equilateral triangular shape, cavity is equipped with in described handle and coating end, temperature sensor is provided with in the cavity of described coating end, temperature indicator and comparator circuit is provided with in the cavity of described handle, described temperature sensor is electrically connected with an input terminus of comparator circuit, another input terminus of comparator circuit connects reference voltage, the output terminal of described comparator circuit is electrically connected with the driving circuit of described temperature indicator.
Preferably, the length on the limit of described coating end is 3 ~ 5cm.
Preferably, the length of described handle is 15 ~ 20cm.
Preferably, the limit of described coating end is rounded, and its diameter is 3 ~ 5mm.
Preferably, the grip part of described handle is provided with anti-skid structure.
Preferably, the angle between described handle and described coating end place plane is 100 ° ~ 180 °.
Above, when the temperature of coating end is more than 37 DEG C, temperature indicator is red, when temperature is at 37 DEG C or following, the color of temperature indicator is green, can arrive judge that whether coating end is in the safety temperature of bacterial organisms fast according to the color experimenter of temperature indicator.
Principle of work of the present invention is as follows: temperature sensor exports corresponding voltage according to current temperature, and current magnitude of voltage is compared with the magnitude of voltage preset, if higher than the magnitude of voltage preset, comparer then exports high level, and driving circuit (red) starts, and sends ruddiness; If current magnitude of voltage is lower than the magnitude of voltage preset, then comparer output low level, driving circuit (green) starts, and sends green glow.
Because technique scheme is used, the present invention compared with prior art has following advantages:
1. the present invention is coated with end in equilateral triangle shape, while guarantee bacterium liquid Painting effect, also improves the stability that bacterium spreading rod slides in culture dish;
2. one aspect of the present invention can prevent the too high and boiling hot dead bacteria effect experimental result of temperature being coated with end, can judge that whether coating end is in safety temperature according to temperature indicator on the other hand, greatly shorten the waiting time, the accuracy of increasing work efficiency and testing;
3. the grip part of handle of the present invention is provided with anti-skid structure, can increase frictional force, ensures the stability of operation;
4. structure of the present invention is simple, and cost is low, is convenient to promote the use of.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is the side-view of the embodiment of the present invention one;
Fig. 3 is the structural representation of the comparator circuit of the embodiment of the present invention one.
Wherein: 1, handle; 2, coating end; 3, temperature sensor; 4, temperature indicator; 5, comparator circuit.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described:
Embodiment one: shown in Figure 1, a kind of bacterium spreading rod with temperature sensor, the coating end 2 comprising handle 1 and be connected with handle 1, handle 1 and coating end 2 are structure as a whole, coating end 2 is in equilateral triangular shape, cavity is equipped with in handle 1 and coating end 2, temperature sensor 3 is provided with in the cavity of coating end 2, temperature indicator 4 and comparator circuit 5 is provided with in the cavity of handle, temperature sensor 3 is electrically connected with an input terminus of comparator circuit 5, another input terminus of comparator circuit 5 connects reference voltage, the output terminal of comparator circuit 5 is electrically connected with the driving circuit of temperature indicator 4.
In the present embodiment, reference voltage can adopt divider resistance to obtain, the output valve coupling of the temperature sensor that the value of reference voltage is corresponding with preset temp; Described temperature indicator can adopt dual-colored LED.
In the present embodiment, the length on the limit of coating end 2 is 4cm.
In the present embodiment, the length of handle 1 is 18cm.
In the present embodiment, the limit of coating end 2 is rounded, and its diameter is 4mm.
In the present embodiment, the grip part of handle 1 is provided with anti-skid structure.
In the present embodiment, shown in Figure 2, the angle between handle 1 and coating end 2 place plane is 120 °.
In the present embodiment, temperature sensor 3 is long strip shape temperature sensor.
Above, when the temperature of coating end is more than 37 DEG C, temperature indicator 4 is red, when temperature is at 37 DEG C or following, the color of temperature indicator 4 is green, can arrive judge that whether coating end is in the safety temperature of bacterial organisms fast according to the color experimenter of temperature indicator 4.
The present embodiment is also provided with a sterilization refrigeration cassette matched with bacterium spreading rod, at ordinary times using the conserving case of sterilization refrigeration cassette as bacterium spreading rod, bacterium spreading rod is made well to be managed preservation, because handle and coating end are in certain angle, be placed on outside to be easy to fracture, be placed in this receiver and can effectively prevent bacterium spreading rod from fractureing.During coating experiment, during coating experiment, refrigeration switch can be opened, make the temperature of receiver be down to design temperature rapidly, on the other hand, be furnished with ultraviolet lamp in receiver and can play sterilization effect, also be furnished with head light, make the visual field more bright clear, so that experimental implementation.
Principle of work of the present invention is as follows: shown in Figure 3, and temperature sensor exports corresponding voltage according to current temperature, and current magnitude of voltage is compared with the magnitude of voltage preset, if higher than the magnitude of voltage preset, comparer then exports high level, and driving circuit (red) starts, and sends ruddiness; If current magnitude of voltage is lower than the magnitude of voltage preset, then comparer output low level, driving circuit (green) starts, and sends green glow.

Claims (6)

1. the bacterium spreading rod with temperature sensor, the coating end (2) comprising handle (1) and be connected with handle (1), described handle (1) and coating end (2) are structure as a whole, described coating end (2) is in equilateral triangular shape, it is characterized in that: in described handle (1) and coating end (2), be equipped with cavity, temperature sensor (3) is provided with in the cavity of described coating end (2), temperature indicator (4) and comparator circuit (5) is provided with in the cavity of described handle, described temperature sensor (3) is electrically connected with an input terminus of comparator circuit (5), another input terminus of comparator circuit (5) connects reference voltage, the output terminal of described comparator circuit (5) is electrically connected with the driving circuit of described temperature indicator (4).
2. the bacterium spreading rod with temperature sensor according to claim 1, is characterized in that: the length on the limit of described coating end (2) is 3 ~ 5cm.
3. the bacterium spreading rod with temperature sensor according to claim 1, is characterized in that: the length of described handle (1) is 15 ~ 20cm.
4. the bacterium spreading rod with temperature sensor according to claim 1, is characterized in that: the limit of described coating end (2) is rounded, and its diameter is 3 ~ 5mm.
5. the bacterium spreading rod with temperature sensor according to claim 1, is characterized in that: the grip part of described handle (1) is provided with anti-skid structure.
6. the bacterium spreading rod with temperature sensor according to claim 1, is characterized in that: described handle (1) and described coating hold the angle between the plane of (2) place to be 100 ° ~ 180 °.
CN201510238971.4A 2015-05-12 2015-05-12 Bacterium coating rod provided with temperature sensor Pending CN104805003A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510238971.4A CN104805003A (en) 2015-05-12 2015-05-12 Bacterium coating rod provided with temperature sensor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510238971.4A CN104805003A (en) 2015-05-12 2015-05-12 Bacterium coating rod provided with temperature sensor

Publications (1)

Publication Number Publication Date
CN104805003A true CN104805003A (en) 2015-07-29

Family

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Family Applications (1)

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CN201510238971.4A Pending CN104805003A (en) 2015-05-12 2015-05-12 Bacterium coating rod provided with temperature sensor

Country Status (1)

Country Link
CN (1) CN104805003A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202786247U (en) * 2012-09-29 2013-03-13 四川农业大学 Digital temperature displaying coating rod
CN203128572U (en) * 2013-03-21 2013-08-14 中国水产科学研究院东海水产研究所 Coating rod capable of automatically indicating cooling degree
CN203546024U (en) * 2013-10-18 2014-04-16 郑州牧业工程高等专科学校 Bacterial spreader capable of displaying temperatures
CN104513792A (en) * 2013-12-13 2015-04-15 成都欣华源科技有限责任公司 Variable color bacterium spreading rod
CN204752727U (en) * 2015-05-12 2015-11-11 苏州大学 Bacterium coating rod with temperature sensor

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202786247U (en) * 2012-09-29 2013-03-13 四川农业大学 Digital temperature displaying coating rod
CN203128572U (en) * 2013-03-21 2013-08-14 中国水产科学研究院东海水产研究所 Coating rod capable of automatically indicating cooling degree
CN203546024U (en) * 2013-10-18 2014-04-16 郑州牧业工程高等专科学校 Bacterial spreader capable of displaying temperatures
CN104513792A (en) * 2013-12-13 2015-04-15 成都欣华源科技有限责任公司 Variable color bacterium spreading rod
CN204752727U (en) * 2015-05-12 2015-11-11 苏州大学 Bacterium coating rod with temperature sensor

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Application publication date: 20150729