CN104801539B - Reduction dechlorination enzyme is utilized to repair the method in contaminated with halogenated hydrocarbons place - Google Patents
Reduction dechlorination enzyme is utilized to repair the method in contaminated with halogenated hydrocarbons place Download PDFInfo
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- CN104801539B CN104801539B CN201510177047.XA CN201510177047A CN104801539B CN 104801539 B CN104801539 B CN 104801539B CN 201510177047 A CN201510177047 A CN 201510177047A CN 104801539 B CN104801539 B CN 104801539B
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Abstract
The present invention relates to a kind of contaminated site restorative procedure, be mainly used in the reparation that contaminated with halogenated hydrocarbons thing is main contaminated site, be specifically related to use the reparation of reduction dechlorination enzyme.The present invention utilizes reduction dechlorination enzyme to the special highly effective of contaminated with halogenated hydrocarbons thing, proposes a kind of method extending the activity of reduction dechlorination enzyme, reaches fast, thoroughly to remove contaminated site depths halogenated hydrocarbons DNAPLs pollutant with this.The method utilizing reduction dechlorination enzyme to repair contaminated with halogenated hydrocarbons place provided by the invention, comprise the underground that biology enzyme is injected into contaminated with halogenated hydrocarbons place and be delivered to pollution sources region degradation of contaminant, it is characterized in that: described biology enzyme is reduction dechlorination enzyme, and reduction dechlorination enzyme is prepared into liquid enzyme preparation, containing reduction dechlorination enzyme, diethyl aminoethyl dextran and high molecular alcohol in enzyme preparation.In reduction dechlorination enzyme preparation prepared by invention, the activity of enzyme can maintain a week more than, ensure that the practicality for site remediation.
Description
Technical field
The present invention relates to a kind of contaminated site restorative procedure, be mainly used in the reparation that contaminated with halogenated hydrocarbons thing is main contaminated site, be specifically related to use the reparation of reduction dechlorination enzyme.
Background technology
As conventional industrial chemicals, halogenated hydrocarbons is used as organic solvent and is used in the industries such as dry-cleaning, machining and electronics processing in a large number.A large amount of halogenated hydrocarbons can be revealed or be released in environment with the form of discarded object after a procedure from the conservation tank being embedded in underground.Halogenated hydrocarbons produces carcinogenic, teratogenesis, mutagenic three-induced effect to human body, has extremely strong toxicity, thus once be released in environment, brings greater risk can to health and Environment Ecological Safe.Halogenated hydrocarbons is because its density ratio water is large, solubility is relatively little, therefore, to sink in underground reservoir after being discharged with the form of discarded object or revealing from subterranean reservoir tank when halogenated hydrocarbons always and form a kind of heavy nonaqueous phase liquid (densenon-aqueousphaseliquid, DNAPL), discharge halogenated hydrocarbons organic matter in body of groundwater towards periphery lentamente as a kind of long-time pollution source, cause downstream underground water pollution.
The restorative procedure in common contaminated with halogenated hydrocarbons place has gas phase extraction process (SVE), biological restoration, chemical oxidation/reduction repairing method and plant restoration method etc.Because contaminated with halogenated hydrocarbons thing is present in the underground reservoir of contaminated site with the form of DNAPLs usually, in above-mentioned common restorative procedure use reagent or gas to be all comparatively difficult to touch DNAPLs pollution sources, therefore, usually can not reach reparation requirement.Plant and microorganism remediation contaminated site method are all by enzyme effect degradable organic pollutant or reduction curing heavy metal contaminants in essence after all.Biology enzyme, because having the characteristic of very strong single selective and degradation of contaminant fast, is widely used in the improvement of multiple sewage.Patent (CN101214493B) reports the method for the DDT utilized in laccase oxidation stain soil, mainly remain in the DDT in soil by laccase being added to direct catalytic degradation in contaminated soil, require that soil moisture remains on 15%, found that after first day and middle the 11 day respectively add the laccase of 3ug in every gram of soil, repair after 25 days, the concentration of DDT in soil has been reduced to below the half of initial concentration.But the laccase that the method uses is a kind of oxidizing ferment, and DDT is difficult to by exhaustive oxidation, and meanwhile, the activity of biology enzyme is shorter, have impact on the result of use used it for contaminated site and repair.In addition, the method is only limitted to use laccase to repair contaminated soil, is difficult to produce repairing effect for being easy to have deposited to contaminated site depths to the contaminated with halogenated hydrocarbons thing in water-bearing layer.
Summary of the invention
In view of the above-mentioned multiple recovery technique mentioned is to the various deficiencies of halogenated hydrocarbons DNAPLs contaminated site, the invention provides a kind of method fast, thoroughly can removing contaminated site depths halogenated hydrocarbons DNAPLs pollutant.
The present invention utilizes reduction dechlorination enzyme to the special highly effective of contaminated with halogenated hydrocarbons thing, proposes a kind of method extending the activity of reduction dechlorination enzyme, reaches fast, thoroughly to remove contaminated site depths halogenated hydrocarbons DNAPLs pollutant with this.
The method utilizing reduction dechlorination enzyme to repair contaminated with halogenated hydrocarbons place provided by the invention, comprise the underground that biology enzyme is injected into contaminated with halogenated hydrocarbons place and be delivered to pollution sources region degradation of contaminant, it is characterized in that: described biology enzyme is reduction dechlorination enzyme, and reduction dechlorination enzyme is prepared into liquid enzyme preparation, containing reduction dechlorination enzyme, diethyl aminoethyl dextran and high molecular alcohol in enzyme preparation.
Consider that the reduction dechlorination enzyme preparation being injected into underground, contaminated with halogenated hydrocarbons place has enough responds and its activity can be maintained the sufficiently long time, and consider cost factor, therefore, the mass content preferable range of the reduction dechlorination enzyme in the reduction dechlorination enzyme enzyme preparation of preparation is at 100mg/L-3g/L, diethyl aminoethyl dextran and the high molecular alcohol total mass content preferable range in enzyme preparation is at 2-8g/L, and all the other compositions are water.
Because the pH value of biology enzyme to temperature and residing water environment has very strong sensitiveness, when lower temperature, biology enzyme shows activity hardly, and temperature higher time, activity also can greatly be weakened, therefore, the preferred suitable environment temperature range of the reduction dechlorination enzyme enzyme preparation in invention is 10 DEG C-40 DEG C, and the pH value range of water environment is at 4-8.5.
Because biology enzyme has specific single-minded selective and high reaction activity to pollutant, when repairing for contaminated site, the object that can realize fast, thoroughly repair.Reduction dechlorination enzyme has specifically selective and high activity and the halogen atom in halogenated hydrocarbons thoroughly can being reduced to halogenated hydrocarbons and removes, and finally generates nontoxic hydro carbons.Reduction dechlorination enzyme is dissolved in the mixed liquor of diethyl aminoethyl dextran and high molecular alcohol be prepared into enzyme preparation can the activity (as a week) of long term maintenance reduction dechlorination enzyme, and then contaminated site underground can be injected into and be sent to the contaminated with halogenated hydrocarbons source region at contaminated site deep layer place, and then reach fast, eliminate the object of contaminated with halogenated hydrocarbons thing up hill and dale.
In order to ensure that reduction dechlorination enzyme preparation is after being injected into underground, contaminated with halogenated hydrocarbons place, reduction dechlorination enzyme can be transported to contaminated with halogenated hydrocarbons source region quickly and efficiently, when the reduction dechlorination enzyme preparation of inventing to contaminated with halogenated hydrocarbons place subsurface injection, preferred injection rate scope is at 1L/min-20L/min.
The details of use of reduction dechlorination enzyme preparation provided by the invention are described below by way of detailed description of the invention.
Detailed description of the invention
Embodiment 1
The reduction dechlorination enzyme that utilizes provided in the present invention repairs the method in contaminated with halogenated hydrocarbons place, implements according to following steps:
1) contaminated site investigation finds, the area of target stains contaminated sites source region is 20m
2, pollution depth is 8m, and pollutant is that in the pollutant place of the mixture of trichloro-ethylene and tetrachloro-ethylene, in the underground water of contaminated site, the concentration of trichloro-ethylene and tetrachloro-ethylene is respectively 215mg/L and 183mg/L, and finds that there is chlorine halocarbon DNAPLs and exist;
2) upstream end of the underground water in the pollution sources region of contaminated site excavates two injector wells, and the underground water downstream part under pollution sources region excavates two injector wells, has a detection well middle excavation of injector well and extraction well.Injector well, extraction well and the detection well spacing degree of depth are overhead 8m;
3) on above-mentioned contaminated site, the commercial 2000g reduction dechlorination enzyme buied is added to (wherein the total mass content of diethyl aminoethyl dextran and high molecular alcohol is 5g/L) in the diethyl aminoethyl dextran of 2000L and the mixture of high molecular alcohol, mixing, preparing containing reduction dechlorination enzyme concentration is the enzyme preparation of 1g/L, for subsequent use;
4) above-mentioned for 2000L enzyme preparation is injected into pollution sources region by peristaltic pump by two mouthfuls of injector wells in above-mentioned pollution sources region, every mouthful of well is all with 5L/min.While injection enzyme preparation, need by two mouthfuls of extraction well extracting underground water in above-mentioned pollution sources region, the extracting speed of every mouthful of well is also 5L/min, stops extracting underground water immediately after having injected;
5) from detection well, get sampling of ground water after injection process and injection, wherein get the frequency of sampling of ground water for once a day, the trichloro-ethylene in the sampling of ground water that analysis is got and the concentration of tetrachloro-ethylene.Found that, injecting reparation after 24 days, in underground water, the concentration of trichloro-ethylene and tetrachloro-ethylene is reduced to 15mg/L and 12mg/L respectively, divide the degradation effect being clipped to 93.1% and 93.4%, and inject reparation after 24 days, do not find the metabolic intermediate of trichloro-ethylene or tetrachloro-ethylene: dichloroethylene and vinyl chloride.
Claims (2)
1. the method utilizing reduction dechlorination enzyme to repair contaminated with halogenated hydrocarbons place, comprise the underground that biology enzyme is injected into contaminated with halogenated hydrocarbons place and be delivered to pollution sources region degradation of contaminant, it is characterized in that: described biology enzyme is reduction dechlorination enzyme, and reduction dechlorination enzyme is prepared into liquid enzyme preparation, containing reduction dechlorination enzyme in enzyme preparation, diethyl aminoethyl dextran and the mass content scope of the said reduction dechlorination enzyme of high molecular alcohol in enzyme preparation are at 100mg/L-3g/L, said diethyl aminoethyl dextran and the high molecular alcohol total mass content scope in enzyme preparation is at 2-8g/L, all the other compositions are water, the suitable environment temperature of said enzyme preparation is 10 DEG C-40 DEG C, the pH value range of water environment is at 4-8.5.
2., according to a kind of method utilizing reduction dechlorination enzyme to repair contaminated with halogenated hydrocarbons place according to claim 1, it is characterized in that: the injection rate scope of reduction dechlorination enzyme preparation is at 1L/min-20L/min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510177047.XA CN104801539B (en) | 2015-04-15 | 2015-04-15 | Reduction dechlorination enzyme is utilized to repair the method in contaminated with halogenated hydrocarbons place |
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| CN201510177047.XA CN104801539B (en) | 2015-04-15 | 2015-04-15 | Reduction dechlorination enzyme is utilized to repair the method in contaminated with halogenated hydrocarbons place |
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| CN104801539A CN104801539A (en) | 2015-07-29 |
| CN104801539B true CN104801539B (en) | 2016-04-27 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005245344A (en) * | 2004-03-05 | 2005-09-15 | Kokusai Kogyo Co Ltd | Method for forecasting natural attenuation capacity of polluting material |
| CN101214493A (en) * | 2007-12-29 | 2008-07-09 | 华南农业大学 | Method for repairing DDT contaminated soil |
| CN103333823A (en) * | 2013-06-14 | 2013-10-02 | 大连理工大学 | Preparation method and application of microbial inoculant for remediating poly-halohydrocarbon-polluted soil |
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2015
- 2015-04-15 CN CN201510177047.XA patent/CN104801539B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005245344A (en) * | 2004-03-05 | 2005-09-15 | Kokusai Kogyo Co Ltd | Method for forecasting natural attenuation capacity of polluting material |
| CN101214493A (en) * | 2007-12-29 | 2008-07-09 | 华南农业大学 | Method for repairing DDT contaminated soil |
| CN103333823A (en) * | 2013-06-14 | 2013-10-02 | 大连理工大学 | Preparation method and application of microbial inoculant for remediating poly-halohydrocarbon-polluted soil |
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| CN104801539A (en) | 2015-07-29 |
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Effective date of registration: 20190305 Address after: 251100 Qilu high tech Development Zone, Qihe County, Dezhou, Shandong Patentee after: Shandong Bi Quan Environmental Engineering Technology Co., Ltd. Address before: Room 108, Training Base Office Building, Shandong Vocational College, 62 Jiefang Road, Jinan City, Shandong Province Patentee before: Liu Xiaoyong |
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Denomination of invention: Method for repairing halogenated hydrocarbon contaminated site by reducing dechlorination enzyme Effective date of registration: 20211119 Granted publication date: 20160427 Pledgee: Shandong Qihe Rural Commercial Bank Co.,Ltd. Pledgor: SHANDONG BIQUAN ENVIRONMENT ENGINEERING TECHNOLOGY Co.,Ltd. Registration number: Y2021980012821 |
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