CN104801536B - Chemical leaching is utilized to be combined the method repairing contaminated with halogenated hydrocarbons place with microbial - Google Patents
Chemical leaching is utilized to be combined the method repairing contaminated with halogenated hydrocarbons place with microbial Download PDFInfo
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- CN104801536B CN104801536B CN201510177096.3A CN201510177096A CN104801536B CN 104801536 B CN104801536 B CN 104801536B CN 201510177096 A CN201510177096 A CN 201510177096A CN 104801536 B CN104801536 B CN 104801536B
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Abstract
The present invention relates to a kind of method utilizing chemical leaching to be combined reparation contaminated with halogenated hydrocarbons place with microbial, be specifically related to use ELUTION METHOD and repairing method of microorganism. The restorative procedure in contaminated with halogenated hydrocarbons place provided by the invention, method includes using chemical leaching, it is that leacheate is injected contaminated soil by injector well, downstream at contaminated site, the leacheate carrying pollutant is extracted out from extraction well, it is characterized in that: the leacheate adopted be mainly composed of the biosurfactant being dissolved in alcohol-water mixed solution, and after adopting leacheate drip washing, KB1 bacterium suspension is expelled in contaminated soil and carries out microorganism remediation. Chemical method owing to using carries out drip washing and can quickly realize the place of high concentration contaminated with halogenated hydrocarbons is carried out early stage reparation, subsequently, adopt microorganism remediation can solve the chemical leaching technology drawback to there will be pollutant levels bounce-back after low concentration pollutant poor effect and drip washing, and can reach thoroughly to repair the purpose of contaminated with halogenated hydrocarbons thing.
Description
Technical field
The present invention relates to the restorative procedure in a kind of contaminated with halogenated hydrocarbons place, be specifically related to use ELUTION METHOD and repairing method of microorganism.
Background technology
Day by day serious soil pollution, causes high risks to grain security and ecological safety. The numerous materials causing soil pollution include heavy metal, organic pollution, pesticide, herbicide, POPs and chemical fertilizer etc. These pollutant are not only in upper soll layer deposition enrichment, and can be diffused in the water-bearing layer under soil horizon, and then polluted underground water, cause bigger health risk. And in multiple pollutant, halogenated organic compounds is because its distinctive density ratio water is big and dissolubility in water is little etc. specific, it is easier to deposit to underground reservoir and forms a kind of material (densenon-aqueousphaseliquids being called dense non-aqueous phase liquids, DNAPLs), and longer-term persistence in water-bearing layer as pollution source of groundwater. Cause toxic owing to halogenated organic compounds has " carcinogenic, teratogenesis, mutagenesis " three, bring very big potential safety hazard to the drinking water of the periphery subsoil water of contaminated site and Lower Reaches. Therefore, the subsoil water repaired in contaminated with halogenated hydrocarbons place is a major issue needing solution badly.
Reparation for contaminated with halogenated hydrocarbons place, major technique has bioremediation technology, chemical oxidation/reduction technique, the gas phase extraction technique of improvement, chemical leaching technology etc., but these technology are Shortcomings all more or less, as microorganism would generally be produced to poison because of the excessive concentration of halogenated hydrocarbons dirt by bioremediation technology, and then weaken the effect of biological restoration; Chemical oxidation/reduction technique is then it is difficult to ensure that the chemical reagent injected accurately arrives at the polluter in water-bearing layer, and chemical reagent is generally shorter in the time of staying of pollution source area simultaneously, it is impossible to meet the demand of fully oxidized for pollutant/reduction;In chemical leaching process, chemical leaching can form preferential flow paths footpath at underground reservoir after injecting underground reservoir, causes that the pollutant in hypotonicity regional area can not be leached out, and, there will be pollutant levels bounce-back after drip washing, finally have influence on drip washing effect. Additionally, the drip washing less effective that chemical leaching is to low concentration pollutant.
Summary of the invention
Based on above prior art Problems existing, the present invention proposes the restorative procedure in a kind of contaminated with halogenated hydrocarbons place, and the method can reach quickly, repair up hill and dale the purpose in contaminated with halogenated hydrocarbons place.
The method utilizing chemical leaching to be combined reparation contaminated with halogenated hydrocarbons place with microbial provided by the invention, including using chemical leaching, it is that leacheate is injected contaminated soil by injector well, downstream at contaminated site, the leacheate carrying pollutant is extracted out from extraction well, it is characterized in that: the leacheate adopted be mainly composed of the biosurfactant being dissolved in alcohol-water mixed solution, and after adopting leacheate drip washing, KB1 bacterium suspension is expelled in contaminated soil and carries out microorganism remediation. Wherein KB1 bacterium is mainly dehalococcoides (microorganism two kinds not of the same clan) and acetogens and methanogens mushroom.
Due to the fact that employing chemical method carries out drip washing, can quickly realize the place of high concentration contaminated with halogenated hydrocarbons is carried out early stage reparation, subsequently, adopt microorganism remediation can solve the chemical leaching technology drawback to there will be pollutant levels bounce-back after low concentration pollutant poor effect and drip washing, and can reach thoroughly to repair the purpose of contaminated with halogenated hydrocarbons thing. Simultaneously, owing to the present invention employs the leacheate containing biosurfactant in the chemical leaching stage, ensure on the one hand from contaminated soil, effectively dissolve halogenated hydrocarbons and by extracting, halogenated hydrocarbons is departed from contaminated soil, on the other hand, inject the leacheate containing biosurfactant can remain in contaminated site, and as the carbon source of later stage microorganism remediation, promote that microbial growth is to promote the microorganism reparation to halogenated hydrocarbons. KB1 bacterium used in invention is the special bacteria of a kind of contaminated with halogenated hydrocarbons thing of can degrading quickly and efficiently. The KB1 microorganism that this method middle and late stage injects make use of the biosurfactant being retained in place in early stage lessivation, KB1 microorganism to be expedited the emergence of and can effectively eliminate the halo organic pollution of the left low concentration of antecedent chemical lessivation. The present invention utilizes the combination of ELUTION METHOD and repairing method of microorganism, it is achieved that quickly, up hill and dale repair contaminated with halogenated hydrocarbons place purpose.
Alcohols is always as cosolvent good in leacheate, it is widely used in contaminated site reparation, simultaneously, owing to the density of ethylene glycol and glycerol is a bit larger tham water, sinking effect somewhat is had after being injected into underground, being conducive to driving leacheate and be transferred through contaminated with halogenated hydrocarbons source region (generally existing with DNAPLs form at contaminated site), therefore, the preferred version of the present invention is: the described alcohol in alcohol-water mixtures at least contains one or several in ethylene glycol and glycerol.
Considering drip washing effect and the cost factor of leacheate, the preferred embodiments of the present invention is: it is 20-30% that the described biosurfactant in leacheate accounts for the total mass ratio of leacheate, and it is 20-30% that the quality of alcohols accounts for the total mass ratio of leacheate, and all the other are water.
Accompanying drawing explanation
Fig. 1 is the technique exemplary view of combination biosurfactant drip washing and biotechnology DNA plerosis PLs contaminated site.
1. peristaltic pump in figure, 2. ground, 3. Groundwater flow path direction, 4. groundwater level, 5. free aquifer, 6. sealing coat, 7. artesian aquifer, 8. polluter, 9. extract direction, 10. extraction well, 11. extracts, 12 injector wells, the eluent flow direction of 13. injections.
Other details of invention are described below in conjunction with specific embodiment and accompanying drawing.
Detailed description of the invention
1) selecting rhamnose as the biosurfactant in preparation leacheate, selecting glycerol is the alcohols in leacheate, and wherein rhamnose, glycerol and water three account for the ratio of whole leacheate quality respectively 25%, 25% and 50%;
2) site investigation finds, selected target stains place mainly contains chlorohydrocarbon DNAPLs, it is in underground 4-7 rice containing DNAPLs pollution source area 8, phreatic line 4 is 2.5 meters, underground, Groundwater flow path direction 3 is direction eastwards from west, the hydrogeology of contaminated site mainly comprises the lower unsaturation layer 0.8 meter thick of Groundwater bit line, and overhead the degree of depth is the free aquifer 5 of 3.3 meters-8 meters, sealing coat 6 under free aquifer, artesian aquifer 7 under sealing coat, artesian aquifer has again one layer of sealing coat for 7 times, as shown in Figure 1;
3) media particle in polluter region is mainly coarse sand granule and the mixing of fine sand grain, and mean porosities is 0.36, and the area in polluter region is about 1000m3, therefore its pore volume is 360m3;
4) by syringe pump 1 by 7 times of polluter pore volume (i.e. 2520m3) leacheate be expelled to the upstream in polluter region via injector well 12, the degree of depth of injector well 12 is 5.2m, and the border in distance polluter region is 0.5m, and downwardly, injection rate is 10m to the leacheate flow direction 13 of injection3/ h, while injection leacheate, in the downstream in polluter 8 region via the outside extracting subsoil water of extraction well 10, extract 11 is collected on ground 2 and needs subsequent treatment, extraction well overhead 2 the degree of depth be 6.1 meters, the speed of extracting subsoil water with injection leacheate speed identical, be also 10m3/ h;
5) after leacheate to be done injects, continuing the subsoil water in speedup extracting water-bearing layer, this process need to continue pore volume (the i.e. 720m that the volume of the subsoil water of extracting is 2 times of institute's drip washing regions altogether3);
6) subsequently, seal the well lid of extraction well 10, in injector well, inject 720m3Bacterium solution, wherein the main thalline in bacterium solution is KB1 mushroom, and its concentration is 1.8*1011/L-2.2*1011/ L, bacterium solution is through deoxidation treatment, and has dissolved the sodium nitrate of 3500g in bacterium solution;
7) injection rate of bacterium solution is 5m3/ h, while injection bacterium solution, it is not necessary at the downstream extracting subsoil water in polluter region;
8) after having injected bacterium solution, sealing injection well, and groundwater abstraction water sample and gather the soil-like in polluter region, pollutant levels, inorganic salt, NO in detection water sample and soil-like weekly3 -Isoconcentration.
The effect of above-described embodiment is as follows:
Extract is divided into and separates out chlorohydrocarbon (main component is trichloro ethylene and dichloroethylene etc.) 120kg. When drip washing completes, in polluter region the trichloro ethylene in subsoil water and dichloroethylene mean concentration be reduced to 42mg/L and 31mg/L by 489mg/L and the 353mg/L before drip washing. After having injected KB1 bacterium solution 6 months, it has been found that in polluter region, the mean concentration of the trichloro ethylene in subsoil water and dichloroethylene is 5.6mg/L and 3.7mg/L.Continue monitoring to injecting bacterium solution after 1 year, it has been found that pollutant levels continue to reduce, without bounce-back. Meanwhile, after 1 year, in pedotheque, the trichloro ethylene of detection and the mean concentration of dichloroethylene are 5.8mg/kg and 4.4mg/kg. Therefore, this result shows that the restorative procedure for contaminated with halogenated hydrocarbons place provided by the invention can efficiently solve the problem of chemical leaching after stain substrate concentration bounce-back, and can eliminate pollutant thoroughly.
Claims (3)
1. one kind utilizes chemical leaching to be combined the method repairing contaminated with halogenated hydrocarbons place with microbial, including chemical leaching, it is that leacheate is injected contaminated soil by injector well, downstream at contaminated site, the leacheate carrying pollutant is extracted out from extraction well, it is characterized in that: the leacheate main component adopted includes the alcohol-water mixture as carrier fluid, biosurfactant is dissolved in carrier fluid, wherein biosurfactant accounts for the total mass ratio of leacheate is 20-30%, it is 20-30% that the quality of alcohols accounts for the total mass ratio of leacheate, and after adopting leacheate drip washing, KB1 bacterium solution is expelled in contaminated soil and carries out microorganism remediation.
2. the chemical leaching that utilizes described in claim 1 is combined the method repairing contaminated with halogenated hydrocarbons place with microbial, it is characterized in that: the described biosurfactant in leacheate at least contains the one in rhamnose, sodium alginate and sapindoside.
3. the chemical leaching that utilizes described in claim 1 or 2 is combined the method repairing contaminated with halogenated hydrocarbons place with microbial, it is characterized in that: the described alcohol in alcohol-water mixture at least contains the one in ethylene glycol and glycerol.
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Families Citing this family (9)
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| CN106040728B (en) * | 2016-06-03 | 2019-05-17 | 南通国盛环境修复有限责任公司 | Soil petroleum hydrocarbon and heavy-metal composite pollution repair process structure and construction technology |
| CN106180168A (en) * | 2016-09-22 | 2016-12-07 | 北京新源环境有限公司 | The method that plant bionic repairs place soil with chemical leaching, microbial association |
| CN108114978B (en) * | 2017-12-29 | 2019-01-15 | 知合环境（北京）有限责任公司 | A kind of method of the efficient rehabilitating soil of chemistry-microorganism |
| CN109047328A (en) * | 2018-08-01 | 2018-12-21 | 北京高能时代环境技术股份有限公司 | A kind of leacheate and soil remediation method |
| CN110548757A (en) * | 2019-09-05 | 2019-12-10 | 金华北顿生物科技有限公司 | Treatment method of petroleum-polluted soil |
| CN112775173A (en) * | 2020-11-30 | 2021-05-11 | 长沙工研院环保有限公司 | Method for restoring soil and underground water polluted by cyanide by in-situ leaching-ventilation enhanced microorganisms |
| CN112474785A (en) * | 2020-12-28 | 2021-03-12 | 苏州精英环保有限公司 | Method for repairing composite polluted site by combining chemical leaching and microorganisms |
| CN115889442A (en) * | 2021-08-13 | 2023-04-04 | 深圳职业技术学院 | Emergency restoration method for metal-rich biochar treatment of halogenated organic polluted site |
| CN115815311B (en) * | 2023-02-08 | 2023-06-09 | 北京中地泓科环境科技有限公司 | Management and control restoration system and method for polluted underground water |
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| CN102941225A (en) * | 2012-11-26 | 2013-02-27 | 中国科学院南京土壤研究所 | Chemistry-microorganism combination restoration method of polycyclic aromatic hydrocarbons and heavy metal composite contaminated site soil |
| CN104411370A (en) * | 2012-04-18 | 2015-03-11 | 株式会社Adeka | Decomposition accelerator for volatile organic halogen compound |
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| US6143177A (en) * | 1995-04-11 | 2000-11-07 | Arcadis Geraghty & Miller, Inc. | Engineered in situ anaerobic reactive zones |
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| US20020020665A1 (en) * | 2000-06-29 | 2002-02-21 | Bechtel Bwxt Idaho, Llc | Halogenated solvent remediation |
| EP1352977A2 (en) * | 2002-04-10 | 2003-10-15 | Universiteit Gent | Novel Desulfitobacterium strain for the degradation of chloroalkenes |
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Effective date of registration: 20190305 Address after: 251100 Qilu high tech Development Zone, Qihe County, Dezhou, Shandong Patentee after: Shandong Bi Quan Environmental Engineering Technology Co., Ltd. Address before: Room 108, Training Base Office Building, Shandong Vocational College, 62 Jiefang Road, Jinan City, Shandong Province Patentee before: Liu Xiaoyong |
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Effective date of registration: 20190906 Address after: Room 307, Block B, Building 1, Yaogu, Jinan, North Section of Gangxin Third Road, Jinan High-tech Zone, 250000 Shandong Province Patentee after: Shandong Xianquan Environmental Protection Engineering Consulting Co., Ltd. Address before: 251100 Qihe Qilu high tech Development Zone, Dezhou, Shandong Patentee before: Shandong Bi Quan Environmental Engineering Technology Co., Ltd. |