CN104800836B - A kind of Isin glue collagen and its production method and application - Google Patents
A kind of Isin glue collagen and its production method and application Download PDFInfo
- Publication number
- CN104800836B CN104800836B CN201510262006.0A CN201510262006A CN104800836B CN 104800836 B CN104800836 B CN 104800836B CN 201510262006 A CN201510262006 A CN 201510262006A CN 104800836 B CN104800836 B CN 104800836B
- Authority
- CN
- China
- Prior art keywords
- parts
- chinese
- root
- weight
- fruit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000008186 Collagen Human genes 0.000 title claims abstract description 21
- 108010035532 Collagen Proteins 0.000 title claims abstract description 21
- 229920001436 collagen Polymers 0.000 title claims abstract description 19
- 239000003292 glue Substances 0.000 title claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- 239000003814 drug Substances 0.000 claims abstract description 44
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 27
- 238000002360 preparation method Methods 0.000 claims abstract description 21
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 241000756943 Codonopsis Species 0.000 claims abstract description 8
- 241001016310 Epimedium grandiflorum Species 0.000 claims abstract description 8
- 244000268590 Euryale ferox Species 0.000 claims abstract description 8
- 235000006487 Euryale ferox Nutrition 0.000 claims abstract description 8
- 235000011201 Ginkgo Nutrition 0.000 claims abstract description 8
- 244000194101 Ginkgo biloba Species 0.000 claims abstract description 8
- 235000008100 Ginkgo biloba Nutrition 0.000 claims abstract description 8
- 239000009636 Huang Qi Substances 0.000 claims abstract description 8
- 240000001341 Reynoutria japonica Species 0.000 claims abstract description 8
- 235000018167 Reynoutria japonica Nutrition 0.000 claims abstract description 8
- 235000018905 epimedium Nutrition 0.000 claims abstract description 8
- 241000251468 Actinopterygii Species 0.000 claims abstract description 7
- 235000002710 Ilex cornuta Nutrition 0.000 claims abstract description 7
- 241001310146 Ilex cornuta Species 0.000 claims abstract description 7
- 235000010326 Osmanthus heterophyllus Nutrition 0.000 claims abstract description 7
- 241000427159 Achyranthes Species 0.000 claims abstract description 5
- 241000132012 Atractylodes Species 0.000 claims abstract description 5
- 235000003717 Boswellia sacra Nutrition 0.000 claims abstract description 5
- 240000007551 Boswellia serrata Species 0.000 claims abstract description 5
- 235000012035 Boswellia serrata Nutrition 0.000 claims abstract description 5
- 241000282994 Cervidae Species 0.000 claims abstract description 5
- 235000002722 Dioscorea batatas Nutrition 0.000 claims abstract description 5
- 235000006536 Dioscorea esculenta Nutrition 0.000 claims abstract description 5
- 240000001811 Dioscorea oppositifolia Species 0.000 claims abstract description 5
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 claims abstract description 5
- 241000208688 Eucommia Species 0.000 claims abstract description 5
- 239000004863 Frankincense Substances 0.000 claims abstract description 5
- 241000112528 Ligusticum striatum Species 0.000 claims abstract description 5
- 244000241872 Lycium chinense Species 0.000 claims abstract description 5
- 235000015468 Lycium chinense Nutrition 0.000 claims abstract description 5
- 241000521581 Millettia Species 0.000 claims abstract description 5
- 240000000249 Morus alba Species 0.000 claims abstract description 5
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 5
- 244000197580 Poria cocos Species 0.000 claims abstract description 5
- 235000008599 Poria cocos Nutrition 0.000 claims abstract description 5
- 244000226566 Psoralea corylifolia Species 0.000 claims abstract description 5
- 244000046146 Pueraria lobata Species 0.000 claims abstract description 5
- 235000010575 Pueraria lobata Nutrition 0.000 claims abstract description 5
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 claims abstract description 5
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 claims abstract description 3
- 229920001287 Chondroitin sulfate Polymers 0.000 claims abstract description 3
- 229940059329 chondroitin sulfate Drugs 0.000 claims abstract description 3
- 240000001949 Taraxacum officinale Species 0.000 claims abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 241000405414 Rehmannia Species 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims 1
- 208000001132 Osteoporosis Diseases 0.000 abstract description 19
- 230000003993 interaction Effects 0.000 abstract description 2
- 239000008280 blood Substances 0.000 description 9
- 208000024891 symptom Diseases 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 229940079593 drug Drugs 0.000 description 8
- 208000010392 Bone Fractures Diseases 0.000 description 7
- 206010017076 Fracture Diseases 0.000 description 7
- 239000011575 calcium Substances 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 210000003734 kidney Anatomy 0.000 description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
- 229910052791 calcium Inorganic materials 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 208000001164 Osteoporotic Fractures Diseases 0.000 description 3
- 241000245665 Taraxacum Species 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 238000003304 gavage Methods 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 208000000059 Dyspnea Diseases 0.000 description 2
- 206010013975 Dyspnoeas Diseases 0.000 description 2
- 208000036119 Frailty Diseases 0.000 description 2
- 206010003549 asthenia Diseases 0.000 description 2
- 230000037118 bone strength Effects 0.000 description 2
- CADZRPOVAQTAME-UHFFFAOYSA-L calcium;hydroxy phosphate Chemical compound [Ca+2].OOP([O-])([O-])=O CADZRPOVAQTAME-UHFFFAOYSA-L 0.000 description 2
- 210000000038 chest Anatomy 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 210000001624 hip Anatomy 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000004409 osteocyte Anatomy 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 208000027502 Ankle fracture Diseases 0.000 description 1
- 208000020084 Bone disease Diseases 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010010214 Compression fracture Diseases 0.000 description 1
- 206010011703 Cyanosis Diseases 0.000 description 1
- 206010011985 Decubitus ulcer Diseases 0.000 description 1
- 206010051055 Deep vein thrombosis Diseases 0.000 description 1
- 208000005189 Embolism Diseases 0.000 description 1
- 208000008924 Femoral Fractures Diseases 0.000 description 1
- 206010020100 Hip fracture Diseases 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- 208000030136 Marchiafava-Bignami Disease Diseases 0.000 description 1
- 208000029725 Metabolic bone disease Diseases 0.000 description 1
- 208000007101 Muscle Cramp Diseases 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 208000004210 Pressure Ulcer Diseases 0.000 description 1
- 208000002103 Shoulder Fractures Diseases 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 206010047249 Venous thrombosis Diseases 0.000 description 1
- 208000012886 Vertigo Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- -1 and it prepares easy Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000035568 catharsis Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229940084954 dexamethasone 1 mg Drugs 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 210000003414 extremity Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000002758 humerus Anatomy 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 210000002414 leg Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 206010029410 night sweats Diseases 0.000 description 1
- 230000036565 night sweats Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000003359 percent control normalization Methods 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229930002330 retinoic acid Natural products 0.000 description 1
- 108010048734 sclerotin Proteins 0.000 description 1
- 206010039722 scoliosis Diseases 0.000 description 1
- 208000013220 shortness of breath Diseases 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000011285 therapeutic regimen Methods 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 231100000889 vertigo Toxicity 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 230000002618 waking effect Effects 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to a kind of Isin glue collagen and its production method and application, its fish bone collagen albumen, chondroitin sulfate, Chinese medicine preparation are according to 10：3：2 weight is than well mixed；The Chinese medicine preparation is prepared by the raw material of following proportionings：Ginkgo leaf, cultivated land, frankincense, mulberry fruit, deer horn glue, reticulate millettia, Psoralea corylifolia, Poria cocos, the bighead atractylodes rhizome, dandelion, Chinese holly, the Radix Astragali, the fruit of Chinese wolfberry, the fleece-flower root, Chinese yam, Radix Codonopsis, Ligusticum wallichii, wilsonii, the root of kudzu vine, the bark of eucommia, the root of bidentate achyranthes, dried orange peel, Gorgon fruit, barrenwort.Each raw material interaction compatibility of albumen powder of present invention production, can play its synergy, it is adaptable to osteoporosis, have a extensive future.
Description
Technical field
The present invention relates to drug field, it is related to a kind of Isin glue collagen, more particularly to fish bone collagen albumen as main former
The fish collagen protein powder and its production method of material and application.
Background technology
Osteoporosis is osteoporosis, is one group of osteopathy that many reasons cause, and bone tissue has normal calcification, calcium salt
It is in normal rates with matrix, the metabolic bone disease with the characteristics of the reduction of unit volume inner bone tissues amount becomes.In most osteoporosises
In, caused by the reduction of bone tissue increases mainly due to bone absorption.With skeleton pain, it is easy to fracture and is characterized.It is to work as this life
One of most prominent social concern and medical problem in boundary, have caused the extensive concern of the United Nations and countries in the world.Osteoporosis
Disease can cause bone amount to reduce and bone strength decline, then be influenceed by slight external force or other factors, you can fracture, claim sclerotin to dredge
Pine property is fractured.China is the most country of elderly population in the world.Current more than 60 years old old man accounts for total population more than 1.8 hundred million
13%, aging population society is rapidly entered, osteoporosis, osteoporotic fracture caused by aging population society
Patient, 84,000,000 people in 2000；1.4 hundred million people in 2010, account for the 7.9% of total population, and the incidence of disease is in obvious increasing trend.
Osteoporosis, osteoporotic fracture main clinical manifestation：Soreness and weakness of waist and knees, Leg Cramps, skeleton pain, body
Shorten, bow-backed deformity, scoliosis, intolerant to fatigue, limbs fatigue, abnormal heat night sweat etc..Severe patient chest deformity, thoracic cavity diminishes,
Even there are the symptoms such as uncomfortable in chest, shortness of breath, cyanosis, expiratory dyspnea.Its clinical characters：Patient age is big, and constitution is weak, severe symptoms, closes
And disease is more, the course for the treatment of is long, and functional rehabilitation is poor, and disability rate is high, and the death rate is high.Microtrauma can cause vertebral compression fractures, Hip Fracture,
Fracture of distal radius, proximal humeral fracture, fracture of distal end of humerus, ankle fracture, fracture of tibial plateau, Methods for Distal Femoral Fractures etc..
Because activity is reduced or long-term bed, pulmonary infection, urinary system infection contamination, pressure sore, Lower limb deep venous thrombosis, lung are susceptible to
The severe complications such as embolism, MOF and it is dead.Not only to patient and family bring animally with it is spiritual
Great pain, returns patient and family brings the huge spending and heavy financial burden of medical expense.
Western medical treatment aspect：Some line special-purpose medicaments treatment cycles are long, and slowly effect, side effect is big；Some Drugs in Therapy
Potency ratio is unreasonable, and restrictive condition is more, and drug compliance is low；Osteoporotic fracture operative treatment risk is big, and complication is more, takes
With factors such as costlinesses.Some patientss is received nonstandard treatment, or even have selected interruption treatment or abandon treatment, go out
Patient's bone amount continues to reduce in having showed treatment clinical course, bone strength continuous decrease, and slight external force can cause fracture generation or again bone
" returning to poverty " phenomenon of folding, makes patient's disability rate and the death rate substantially increase.Have a strong impact on the whole people and prevented and treated osteoporosis engineering
Exhibition and fitness-for-all planning implementation, have aggravated the focus outstanding problem of aging population society.
The content of the invention
It is real with reference to a large amount of ancestral sides and oneself medical treatment for many years the invention aims to overcome the shortcomings of background technology
Trample, by the research to traditional Chinese medicine, and combine dialectical demonstration, the multi-party length for collecting many families seeks therapeutic regimen, according to
The philosophical thinking and traditional Chinese medicine " Shen controls bone, store essential substances, raw marrow " of the materialist dialectics；" liver governing tendons, store blood, main catharsis "；" spleen master
Change fortune foodstuff essence, to give birth to and bring up muscle "." consumptive disease ", " kidney deficiency ", " kidney deficiency then bone become homeless support to the atrophic debility of bones then ostalgia "；" liver kidney spleen
It is empty ", " then failure of the spinal column to keep in upright position, bone is withered and marrow is few, and it is the atrophic debility of bones to send out ", " its muscles and bones is not flourish ", " easily fracture "." kidney bone Animando actually ",
" marrow biochemistry is active, and bone nourishes sufficient, firm and strong "." qi being the governor of blood, blood being the material foundation of QI ", " stagnation of the circulation of vital energy then blood stasis, gas
Capable then blood "；" with behavior honor, blood is expensive to live to gas "；" not general rule bitterly, no pain by smooth breath "；" knot then dissipate it ", " damage then benefit ",
" treatinging deficiency with tonification "；" stasis of blood is gone, newborn, bone is closed ".Basic theories such as " preventiveing treatment of disease ".Adhere to tonifying kidney and nourishing liver, and and the five internal organs；Enriching yin
Wen Yang, it is qi-blood tonifying；Body fluid and tune, mends marrow life bone；Treat both principal and secondary aspect of disease.
To achieve the above object, the technical solution adopted by the present invention is as follows：
A kind of albumen powder for osteoporosis with Isin glue collagen as principal component, it is by fish bone collagen albumen powder, sulphur
Aching and limp ossein powder, Chinese medicine preparation are according to 10：3：2 weight is than well mixed；
The Chinese medicine preparation is prepared by the raw material of following proportionings：
Ginkgo leaf, prepared rhizome of rehmannia, frankincense, mulberry fruit, deer horn glue, reticulate millettia, Psoralea corylifolia, Poria cocos, the bighead atractylodes rhizome, dandelion, the four seasons
Green grass or young crops, the Radix Astragali, the fruit of Chinese wolfberry, the fleece-flower root, Chinese yam, Radix Codonopsis, Ligusticum wallichii, wilsonii, the root of kudzu vine, the bark of eucommia, the root of bidentate achyranthes, dried orange peel, Gorgon fruit, barrenwort.
Preferably, the Chinese medicine preparation is prepared by the raw material that following weight is matched：
35 parts of ginkgo leaf, 35 parts of prepared rhizome of rehmannia, 35 parts of frankincense, 35 parts of mulberry fruit, 30 parts of deer horn glue,
30 parts of reticulate millettia, 30 parts of Psoralea corylifolia, 30 parts of Poria cocos, 28 parts of the bighead atractylodes rhizome, 28 parts of dandelion,
25 parts of Chinese holly, 25 parts of the Radix Astragali, 25 parts of the fruit of Chinese wolfberry, 25 parts of the fleece-flower root, 22 parts of Chinese yam,
20 parts of Radix Codonopsis, 20 parts of Ligusticum wallichii, 20 parts of wilsonii, 15 parts of the root of kudzu vine, 15 parts of the bark of eucommia,
10 parts of the root of bidentate achyranthes, 10 parts of dried orange peel, 10 parts of Gorgon fruit, 10 parts of barrenwort.
The preparation method of the Chinese medicine preparation is as follows：
It is standby above-mentioned raw materials medicine to be weighed according to above-mentioned weight：
（1）Ginkgo leaf, Chinese holly, the Radix Astragali, the fleece-flower root, Radix Codonopsis, Gorgon fruit, barrenwort is taken to mix in the ratio, plus relatively
In 3 times of ethanol of weight 95% of mixture（Volume ratio）, refluxing extraction 2 times, 0.5 hour every time, extract solution merged, and is concentrated into close
The clear cream for 1.2g/ml is spent, 80 DEG C of drying are ground into powder；
（2）Take residual components plus 3 times of weight waters are decocted twice, 2 hours for the first time, (equally add 3 times of weights within second 1 hour
The water of amount), merge decoction liquor twice, it is the medicinal extract of 1.2g/ml that drying under reduced pressure obtains density, is ground into powder；
（3）By step（1）And step（2）The powder of preparation is well mixed to be obtained final product.
Consumption：3 grams every time of albumen powder, 3 times a day, and one month is a course for the treatment of, continuously takes the 1-3 course for the treatment of.
70-the 80% of organic matter is collagen in bone, when bone is generated it may first have to the sufficient collagen of synthesis
Fiber organizes skeletogenous framework, and the amino acid of collagen, hydroxy-proline are to transport calcium to the fortune of osteocyte in blood plasma
Load instrument, the ossein in osteocyte is the binder of calcium hydroxy phosphate.It together constitutes the master of bone with calcium hydroxy phosphate
Body.After people enters person in middle and old age, worse and worse, quantity is fewer and feweri for the collagen quality in bone, it is impossible to effectively all
Calcium hydroxy phosphate bonding get up, at this moment osteoporosis will occur, Isin glue collagen has been found to be reached via oral
Supplement the effect of soft tissue structure.
The present invention mixes Isin glue collagen and Chinese medicine preparation, by rational compatibility, has given full play to each raw material itself
Characteristic property and effect, the complementation between having taken into account；By each raw material synergy each other, its drug effect
Complement each other, optimal matching has been reached each other.
The component of medicine of the present invention uses natural Chinese medicine material, and it prepares easy, and medicine source is extensively, with low cost, its
Follow the prescriptions principle of the traditional Chinese medical science, verify by clinic application, its reliability evident in efficacy, mild in medicine property and, there is not toxic and side effect,
Have a extensive future.
Specific embodiment
Embodiment 1：
A kind of Isin glue collagen, it is by fish bone collagen albumen, chondroitin sulfate, Chinese medicine preparation according to 10：3：2 weight ratio
It is well mixed；
The Chinese medicine preparation is prepared by the raw material that following weight is matched：
35 parts of ginkgo leaf, 35 parts of cultivated land, 35 parts of frankincense, 35 parts of mulberry fruit, 30 parts of deer horn glue,
30 parts of reticulate millettia, 30 parts of Psoralea corylifolia, 30 parts of Poria cocos, 28 parts of the bighead atractylodes rhizome, 28 parts of dandelion,
25 parts of Chinese holly, 25 parts of the Radix Astragali, 25 parts of the fruit of Chinese wolfberry, 25 parts of the fleece-flower root, 22 parts of Chinese yam,
20 parts of Radix Codonopsis, 20 parts of Ligusticum wallichii, 20 parts of wilsonii, 15 parts of the root of kudzu vine, 15 parts of the bark of eucommia,
10 parts of the root of bidentate achyranthes, 10 parts of dried orange peel, 10 parts of Gorgon fruit, 10 parts of barrenwort.
The preparation method of the Chinese medicine preparation is as follows：
It is standby above-mentioned raw materials medicine to be weighed according to above-mentioned weight：
（1）Ginkgo leaf, Chinese holly, the Radix Astragali, the fleece-flower root, Radix Codonopsis, Gorgon fruit, barrenwort is taken to mix in the ratio, plus relatively
In 3 times of ethanol of weight 95% of mixture（Volume ratio）, refluxing extraction 2 times, 0.5 hour every time, extract solution merged, and is concentrated into close
The clear cream for 1.2g/ml is spent, 80 DEG C of drying are ground into powder；
（2）Take residual components plus 3 times of weight waters are decocted twice, 2 hours for the first time, (equally add 3 times of weights within second 1 hour
The water of amount), merge decoction liquor twice, it is the medicinal extract of 1.2g/ml that drying under reduced pressure obtains density, is ground into powder；
（3）By step（1）And step（2）The powder of preparation is well mixed to be obtained final product.
Consumption：3 grams every time, 3 times a day, and one month is a course for the treatment of, continuously takes the 1-3 course for the treatment of.
Embodiment 2
Zoopery
Experimental animal BABc mouse 40, male and female half and half, cleaning grade, body weight 20-22g, by the People's Hospital of city animal center
There is provided.It is randomly divided into 4 groups, every group 10, i.e. blank control group 1, model control group 2, the medicine group 3, control drug group of embodiment 1
4.In addition to 1 group gives physiological saline, remaining each group gives dexamethasone 1mg/kg, injects once within every three days, intramuscular injection, together
When, combine vitamin A acid gavage, daily consumption 70mg/kg, totally three weeks.
During medication modeling in addition to blank control group and model control group give isometric(al) physiological saline gavage, this hair
The medicine group of bright embodiment 1 is administered using gavage mode, and dosage is 0.5g/kg, and three times a day, control drug group is using the celestial spirit of medicine
Bone preserves capsule particle, and dosage is 0.5g/kg, three times a day.After being administered three weeks, writhing number of times is detected, then put to death mouse, mouse
Disconnected neck takes blood, and centrifuging and taking serum detects S-Ca with automatic biochemistry analyzer（Serum calcium）Content.Referring to table 1 below：
Table 1
| Group | S-Ca（mmol/L） | Writhing number of times（It is secondary/15 minutes） |
| Blank control group | 2.03±0.17 | |
| Model control group | 4.15±1.03 | 55±14 |
| The medicine group of embodiment 1 | 2.47±0.51 | 13±3 |
| Control drug group | 2.85±0.67 | 21±4 |
As seen from Table 1, model group compares with blank group, and S-Ca is raised significantly, and writhing number of times increases, and difference is obvious, explanation
Modeling success.The medicine group of the embodiment of the present invention 1 is substantially better than control drug group, i.e. Determination of Calcium in Serum content and reduces, P<0.01, explanation
Calcium loss reduction, and point out medicine of the present invention to possess preferable analgesic effect.
Embodiment 3
Clinical data
Sufferers of osteoporosis face 131 is selected from clinic case, treatment group and control group is randomly divided into, the people for the treatment of group 66,
Medical history, the state of an illness, age, sex are tried one's best quite in case selected by the people of control group 65, treatment group and control group.
Treatment group gives the albumen powder of the preparation of the embodiment of the present invention 1, and 3 grams every time, 3 times a day, a month course for the treatment of, even
It is continuous to take 2 courses for the treatment of；
Control group uses conventional medicine XIANLING GUBAO JIAONANG（Tongjitang Pharmacy Co., Ltd., Guizhou）.Orally, every time 3
Grain, 2 times a day, is divided into early, late warm water and takes, and the course for the treatment of is two months.
Efficacy assessment standard
Diagnostic criteria
1. Western medicine diagnostic criteria：With reference to the osteoporosis committee of Aged in China association Criteria of Osteoporosis subject group
" Chinese's osteoporosis advises diagnostic criteria (the second original text) " (Chinese osteoporosis magazine, 2000 that in October, 1999 formulates
The 1st phase of volume 6 year)
2. tcm diagnosis standard：According to《New Chinese medicine guideline of clinical investigations》(trial version in 2002) " Chinese medicine is new
The evaluation requirement of the guideline of clinical investigations of medicine treatment osteoporosis " is formulated.Show as：Lumbar vertebrae pain, aching and limp few power, step
Carry out difficult, vertigo, can not be prudent, tongue nature or partially red or light, the symptom, osteoporosis such as thin lingual fur or thin white, deep thready pulse
See above-mentioned disease person.
Efficacy assessment standard：
Reference《Chinese osteoporosis magazine》、《Practical osteology》Deng evaluation.
Cure：Clinical symptoms, sign disappear, and functional rehabilitation is normal, does not recur within more than 1 year；
It is effective：Clinical symptoms, sign substantially mitigate, and function is returned to normal；
Effectively：Clinical symptoms, sign part mitigate；
It is invalid：Continuous treatment more than 30 days, symptom, sign are without change.
Treatment results are shown in Table 2：
Table 2
| Group | Cure | It is effective | Effectively | It is invalid | Total effective rate |
| Treatment group | 26 | 22 | 11 | 7 | 89% |
| Control group | 10 | 18 | 16 | 21 | 67% |
It can be seen that it is in significant difference in terms of cure rate and total effective rate that treatment group compares control group.
Embodiment 4
Model case：
Shen, man, 63 years old.Pain in the back for many years, when it is light when weight, a nearly month aggravation.Pain in the back unbearably, and to double hips and under
Acroanesthesia pain, it is impossible to which stand long sitting and normal waking activity, waist-leg are cold substantially, and left leg is obvious, not good enough, diet one of sleeping
As, urine is normal, and stool is normal.Give embodiment 1 preparation fish collagen protein powder, symptom mitigation after a course for the treatment of, after
Continuous to take symptom portion disappearance after two courses for the treatment of, the state of an illness has not recurred more than a year so far for withdrawal observation, is to cure.
The present invention use above-mentioned each Chinese medicine interaction compatibility, can play its collaboration curing the disease effect, Chinese medicine material used respectively into
/ be interweaved with drug effect and to mutually promote and coordinate efficiency, according to clinical verification, it is adaptable to osteoporosis, the application's
Chinese medicine composition is with low cost, evident in efficacy, has broad application prospects.
Claims (1)
1. a kind of Isin glue collagen, it is characterised in that the Isin glue collagen is by fish bone collagen albumen, chondroitin sulfate, Chinese medicine
Preparation is according to 10：3：Than being well mixed, the Chinese medicine preparation is prepared 2 weight by the raw material that following weight is matched：
35 parts of ginkgo leaf, 35 parts of prepared rhizome of rehmannia, 35 parts of frankincense, 35 parts of mulberry fruit, 30 parts of deer horn glue,
30 parts of reticulate millettia, 30 parts of Psoralea corylifolia, 30 parts of Poria cocos, 28 parts of the bighead atractylodes rhizome, 28 parts of dandelion,
25 parts of Chinese holly, 25 parts of the Radix Astragali, 25 parts of the fruit of Chinese wolfberry, 25 parts of the fleece-flower root, 22 parts of Chinese yam,
20 parts of Radix Codonopsis, 20 parts of Ligusticum wallichii, 20 parts of wilsonii, 15 parts of the root of kudzu vine, 15 parts of the bark of eucommia,
10 parts of the root of bidentate achyranthes, 10 parts of dried orange peel, 10 parts of Gorgon fruit, 10 parts of barrenwort；
The preparation method of the Chinese medicine preparation comprises the following steps：
Each raw material for standby is weighed according to weight：
（1）Ginkgo leaf, Chinese holly, the Radix Astragali, the fleece-flower root, Radix Codonopsis, Gorgon fruit, barrenwort mixing are taken, plus relative to 3 times of weight of mixture
95% ethanol, refluxing extraction 2 times, 0.5 hour every time, extract solution merged, and was concentrated into the clear cream that density is 1.2g/ml, 80 DEG C of bakings
It is dry, it is ground into powder；
（2）Surplus stock is taken, is mixed, plus the decocting of 3 times of weight is boiled twice, 2 hours for the first time, second 1 hour, is merged twice
Decoction liquor, it is the medicinal extract of 1.2g/ml that drying under reduced pressure obtains density, is ground into powder；
（3）By step（1）And step（2）The powder of preparation is well mixed to be obtained final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510262006.0A CN104800836B (en) | 2015-05-21 | 2015-05-21 | A kind of Isin glue collagen and its production method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510262006.0A CN104800836B (en) | 2015-05-21 | 2015-05-21 | A kind of Isin glue collagen and its production method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104800836A CN104800836A (en) | 2015-07-29 |
| CN104800836B true CN104800836B (en) | 2017-07-04 |
Family
ID=53686314
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510262006.0A Active CN104800836B (en) | 2015-05-21 | 2015-05-21 | A kind of Isin glue collagen and its production method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104800836B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053570A (en) * | 2015-08-28 | 2015-11-18 | 安徽丰原马鞍山生物化学有限公司 | Corn gluten meal nutritional solution |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103251671B (en) * | 2012-12-28 | 2015-11-18 | 北京中研同仁堂医药研发有限公司 | A kind of composition and method of making the same containing Chinese medicine increasing bone density |
| CN104127683A (en) * | 2014-08-12 | 2014-11-05 | 崔银方 | Traditional Chinese medicine composition for treating or preventing female postmenopausal osteoporosis |
-
2015
- 2015-05-21 CN CN201510262006.0A patent/CN104800836B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104800836A (en) | 2015-07-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102872299B (en) | Medicine compound for treating osteoporosis and osteoporosis fracture | |
| CN103272177B (en) | Traditional Chinese medicine composition for treating bone fracture | |
| CN104127699B (en) | Beneficial member health preparation and preparation method thereof | |
| CN101972453A (en) | Chinese medicinal liquor for treating rheumatic and rheumatoid arthritis and preparation method thereof | |
| CN102512630A (en) | Traditional Chinese medicine composition for treating gallbladder calculus | |
| CN104491733A (en) | Application of traditional Chinese medicine preparation in preparation of medicines for treating anemia, deficiency of vital energy and debilitation | |
| CN104800836B (en) | A kind of Isin glue collagen and its production method and application | |
| CN104826095B (en) | A kind of chondroitin coproduction albumen powder and its production method and application | |
| CN104825956A (en) | Medicine for preventing and treating stroke and recovering muscles, bones and joints | |
| CN103330837A (en) | Traditional Chinese medicine composition for curing osteoporosis of menopausal women | |
| CN102743440B (en) | Traditional Chinese medicines for treating femoral head necrosis | |
| CN106729429A (en) | A kind of Chinese medicine composition promoting blood circulation and removing blood stasis and preparation method thereof | |
| CN104352946A (en) | Chinese patent medicine preparation for treating osteoporosis | |
| CN104800443A (en) | Bone setting agent with functions of tonifying kidney and regenerating bone marrow | |
| CN105287731A (en) | Externally-applied traditional Chinese medicine preparation for treating hyperostosis and preparation method thereof | |
| CN1087619C (en) | Medicines for treating osteoporosis and their preparing process | |
| CN103386017B (en) | Medlar-nourishing amino acid oral solution for nourishing kidney and replenishing essence and preparation process thereof | |
| CN101618141B (en) | Medicament for preventing and curing avascular necrosis of femoral head and preparation method thereof | |
| CN108066656A (en) | It is a kind of to be used to prevent and treat pharmaceutical composition of senile osteoporosis and preparation method thereof | |
| CN106924673A (en) | A kind of Chinese medicine composition for treating osteoproliferation and its medicament preparation method | |
| CN108721528A (en) | A kind of Chinese medicinal capsule for treating hypertension | |
| CN107441180B (en) | Bone-strengthening traditional Chinese medicine composition and preparation method thereof | |
| CN104758888A (en) | Traditional Chinese medicine composition for treating diabetes | |
| CN1159023C (en) | Bone-strengthening capsule | |
| CN105596895A (en) | Pharmaceutical preparation for treating osteoporosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A fish collagen protein and its production method and application Effective date of registration: 20230427 Granted publication date: 20170704 Pledgee: China Postal Savings Bank Co.,Ltd. Feixian Branch Pledgor: LINYI XINYUHUI BIOLOGICAL TECHNOLOGY CO.,LTD. Registration number: Y2023980039454 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| CP03 | Change of name, title or address |
Address after: No. 9 Xinyuhui Road, Biopharmaceutical Industry Park, Jinyang Village, Huyang Town, Fei County, Linyi City, Shandong Province, 276000 Patentee after: Xinyuhui Biotechnology (Shandong) Co.,Ltd. Country or region after: China Address before: Jinyang Village, Huyang Town, Fei County, Linyi City, Shandong Province, 273400 Patentee before: LINYI XINYUHUI BIOLOGICAL TECHNOLOGY CO.,LTD. Country or region before: China |
|
| CP03 | Change of name, title or address |