CN104792919A - Constant-current thin layer chromatograph - Google Patents
Constant-current thin layer chromatograph Download PDFInfo
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- CN104792919A CN104792919A CN201510255042.4A CN201510255042A CN104792919A CN 104792919 A CN104792919 A CN 104792919A CN 201510255042 A CN201510255042 A CN 201510255042A CN 104792919 A CN104792919 A CN 104792919A
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- folder block
- chromatography cylinder
- constant current
- chromatography
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Abstract
The invention discloses a constant-current thin layer chromatograph and particularly relates to a device capable of performing chromatographic separation, analysis and imaging on a thin layer. The constant-current thin layer chromatograph is characterized in that a box body is a hollow rectangular body, an inlet capable of allowing a chromatography cylinder to be inserted in is formed in the upper end of the box body, and a mounting frame opening for allowing a photographing device to be mounted in is formed in the front end of the box body; a lamp bracket is mounted in the mounting frame opening, and 254-nm lamps, 365-nm mercury lamps and ultraviolet lamp holders are respectively and symmetrically mounted on the two sides of the lamp bracket; a plurality of three-position switches and a DC (Direct Current) power supply plug are mounted on the outer side of the box body at the lower end of the mounting frame opening; a time control panel is mounted in the box body at the lower end of the mounting frame opening, and a battery rack is mounted at the rear end of the time control panel, and a battery is mounted at the lower end of the battery rack; the photographing device is mounted at the mounting frame opening; the L-shaped chromatography cylinder is made of a quartz plate which is provided with a protruding cavity in the lower bottom end and can be permeated by ultraviolet rays, an upper end opening of the chromatography cylinder is a frosted surface, and a chromatography plate combining device is mounted at the upper end of the L-shaped chromatography cylinder; the chromatography plate combining device structurally comprises a main clamping block, an auxiliary clamping block, a limiting block, a constant current block, a wetness control bar and a chromatography plate.
Description
Technical field
The present invention relates to a kind of constant current thin layer chromatograph, specifically a kind of can to the device of TLC separation, analysis, imaging.
Background technology
Thin-layer chromatography is conventional organism separation method, moves on to outside chromatography cylinder when developping agent line reaches the top by chromatoplate, under multi-wavelength excites, detects amount and the chemical constitution of the hot spot point launched.Chromatoplate move on to chromatography cylinder outer after can not chromatography in the same way again, when developping agent line reaches the top, developer flow prompt drop is low, and Tomography Velocity is non-constant, and degree of separation reduces, and cannot continue the component being separated long retention time.Continuing the longer chromatography time obtains the key problem that high separation is chromatography, in order to reach satisfied degree of separation, needs Real-Time Monitoring to analyse the state of layer.
Summary of the invention
In order to overcome above-mentioned deficiency, the invention provides a kind ofly constant current can be kept to be separated to thin-layer chromatography, analyze, constant current thin layer chromatograph that imaging is carried out simultaneously.
The technical solution adopted for the present invention to solve the technical problems is: its structure of constant current thin layer chromatograph is: casing is hollow, rectangular body, its upper end is provided with the import can assigning chromatography cylinder, front end is provided with the installing frame mouth installing camera head, and bottom is respectively equipped with several cabinet footing, be equipped with lamp bracket in installing frame mouth, the both sides of lamp bracket respectively symmetry are equipped with 254nm lamp, 365nm mercury lamp and ultraviolet lamp socket, several three-position switch and DC attaching plug is equipped with respectively outside the casing of installing frame mouth lower end, be equipped with time controling plate in the casing of installing frame mouth lower end, time controling plate rear end is equipped with battery carrier, and battery carrier lower end is equipped with battery, installing frame mouth is provided with camera head, L-type chromatography cylinder is that beneath end band has can making by uv-transmitting quartz plate of protrusion cavity, its upper port is frosting, L-type chromatography cylinder upper end is equipped with chromatoplate combination unit, the structure of chromatoplate combination unit is: comprise main folder block, sub-folder block, limited block, constant current block, the wet rod of control and chromatoplate, both sides, main folder block lower end are respectively equipped with two fixed blocks, distance between two pieces is identical with upper port in L-type chromatography cylinder, side, main folder block upper end is provided with platform, platform side is vertically provided with the through hole installing the wet rod of control, main folder block main body side is provided with the trough with straight angle installing sub-folder block, described sub-folder block is the middle strip cuboid with ramped recesses groove, described limited block is the arc block with interior dog, described constant current block is the rectangular block that 2000 order quartz ceramic for filtration are made, the described control rod that wets is the circular barred body that 2000 order quartz sand filtration plates are made, chromatoplate upper end is attached to main folder block side, constant current block is installed, the trough with straight angle of the main folder block other end installs sub-folder block, two limited blocks are pacified into by the intermediate recess groove of main folder block and sub-folder block and are separately clamped to two ends spacing, chromatoplate, main folder block, constant current block, sub-folder block are jointly fixed together, the wet rod of control is inserted in the hole of main folder block, is inserted by chromatography cylinder combination unit in L-type chromatography cylinder, the frosting of chromatography cylinder upper end scribbles vaseline oil, and upper end covers cylinder cap.
The invention has the beneficial effects as follows: the front-back of L-type quartz chromatography cylinder is by uv-transmitting quartz plate manufacture can pass through ultraviolet, launches hot spot point after making to be excited by the organism of chromatography.The L shape in cylinder bottom portion to be designed for L-type quartz chromatography cylinder before chromatography to L bottom direction 90-degree rotation, makes developping agent balance between gas-liquid two-phase.Constant current block is by 2000 order quartz ceramic for filtration manufactures, and when solvent front reaches the coboundary of expansion plate, the siphon that developping agent is subject to constant current block can continue to launch, and duration of run adds more than 3 times, because adding duration of run, degree of separation R is strengthened.Insert in main folder after the wet rod of the control upper leaching 0.5mL concentrated sulphuric acid, the wet rod of control inserts in main folder, for controlling the moisture in gas phase, produces the effect of controlled humidity.2 254nm ultraviolet source lamp symmetries are distributed on the left and right side frame of lamp seat, in the uniform illuminance of acquisition everywhere of chromatoplate.2 365nm ultraviolet source lamp symmetries are distributed on the left and right side frame of lamp seat, in the uniform illuminance of acquisition everywhere of chromatoplate.4 LED visible light source lamp symmetries are distributed on the corner frame of lamp seat, in the uniform illuminance of acquisition everywhere of chromatoplate.Camera is arranged in the rectangle perforate of front panel, can install the camera of different model in rectangle perforate, imports the terminal such as microcomputer or mobile phone into by USB interface or wireless network.Lamp controls by time control circuit plate, makes a video recording, prevented long Ultraviolet radiation from making the possibility of separated organic substance decomposing, and also extended the life-span of lamp when lamp is bright.By certain hour interval respectively under the exciting of 254nm, 365nm and LED visible light to the shooting of separated organism spot, can transfer in the terminal such as microcomputer or mobile phone and carry out chart adding analysis.After duration of run t1, the 1st spot close to solvent front, shooting picture 1; After duration of run t2, front 1 spot disappears in constant current block, shooting picture 2; After duration of run t3, front 2 spots disappear in constant current block, shooting picture 3; After duration of run tn, a front n spot disappears in constant current block, shooting picture n.The relation available chromatography spectrogram EXPANSION EQUATION FOR STEEL formula of picture 1 to picture n superposes, and obtains the spectrogram of whole spot.The method of available replacing constant current block obtains Micro-monomer.Chromatoplate after the siphon of constant current block can Reusability.Structure is simple, easy to use.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further described.
Fig. 1 is constant current thin layer chromatograph structural representation of the present invention.
Fig. 2 is constant current thin layer chromatograph exploded view of the present invention.
In figure 1, three-position switch, 2, DC attaching plug, 3, casing, 4,254nm lamp, 5,365nm mercury lamp, 6, lamp bracket, 7, ultraviolet lamp socket, 8, L-type chromatography cylinder, 9, chromatoplate, 10, constant current block, 11, cylinder cap, 12, limited block, 13, sub-folder block, 14, main folder block, 15, the wet rod of control, 16, battery carrier, 17, battery, 18, cabinet footing, 19, time controling plate.
Embodiment
As Figure 1-Figure 2, its structure of constant current thin layer chromatograph is: casing 3 is hollow, rectangular body, and its upper end is provided with the import can assigning chromatography cylinder, and front end is provided with the installing frame mouth installing camera head, and bottom is respectively equipped with several cabinet footing 18, be equipped with lamp bracket 6 in installing frame mouth, the both sides of lamp bracket 6 respectively symmetry are equipped with 254nm lamp 4,365nm mercury lamp 5 and ultraviolet lamp socket 7, several three-position switch 1 and DC attaching plug 2 is equipped with respectively outside the casing 3 of installing frame mouth lower end, be equipped with time controling plate 19 in the casing 3 of installing frame mouth lower end, time controling plate 19 rear end is equipped with battery carrier 16, and battery carrier 16 lower end is equipped with battery 17, installing frame mouth is provided with camera head, L-type chromatography cylinder 8 protrudes can making by uv-transmitting quartz plate of cavity for beneath end band has, its upper port is frosting, L-type chromatography cylinder 8 upper end is equipped with chromatoplate combination unit, the structure of chromatoplate combination unit is: comprise main folder block 14, sub-folder block 13, limited block 12, constant current block 10, the wet rod 15 of control and chromatoplate 9, both sides, main folder block 14 lower end are respectively equipped with two fixed blocks, distance between two pieces is identical with upper port in L-type chromatography cylinder 8, side, main folder block 14 upper end is provided with platform, platform side is vertically provided with the through hole installing the wet rod 15 of control, main folder block 14 main body side is provided with the trough with straight angle installing sub-folder block 13, described sub-folder block 13 is the middle strip cuboid with ramped recesses groove, described limited block 12 is the arc block with interior dog, described constant current block 10 is the rectangular block that 2000 order quartz ceramic for filtration are made, the described control rod that wets 15 is the circular barred body that 2000 order quartz sand filtration plates are made, chromatoplate 9 upper end is attached to main folder block 14 side, constant current block 10 is installed, the trough with straight angle of main folder block 14 other end installs sub-folder block 13, two limited blocks 12 are pacified into by the intermediate recess groove of main folder block 14 and sub-folder block 13 and are separately clamped to two ends spacing, chromatoplate 9, main folder block 14, constant current block 10, sub-folder block 13 are fixed together jointly, the wet rod 15 of control is inserted in the hole of main folder block 14, chromatography cylinder combination unit is inserted in L-type chromatography cylinder 8, the frosting of chromatography cylinder 8 upper end scribbles vaseline oil, and upper end covers cylinder cap 11.
Each lamp controls by time controling plate 19, makes a video recording when lamp is bright; Made a video recording to separated organism spot respectively in interval under the exciting of 254nm, 365nm and LED visible light at preset timed intervals.No. 1 three-position switch control 254nm illuminator, No. 2 three-position switch control 365nm illuminators, No. 3 three-position switches control LED light source lamp, and No. 4 three-position switches control the timing sequence luminescence of three kinds of lamps; For detecting the segregation phenomenon of thin layer plate under dynamic chromatography in real time, three-position switch connects utilizing camera interface, obtains result import computer monitor through data line by camera shooting.
Claims (1)
1. a constant current thin layer chromatograph, is characterized in that: casing (3) is hollow, rectangular body, and its upper end is provided with the import can assigning chromatography cylinder, and front end is provided with the installing frame mouth installing camera head, and bottom is respectively equipped with several cabinet footing (18), be equipped with lamp bracket (6) in installing frame mouth, the both sides of lamp bracket (6) respectively symmetry are equipped with 254nm lamp (4), 365nm mercury lamp (5) and ultraviolet lamp socket (7), casing (3) outside of installing frame mouth lower end is equipped with several three-position switch (1) and DC attaching plug (2) respectively, be equipped with time controling plate (19) in the casing (3) of installing frame mouth lower end, time controling plate (19) rear end is equipped with battery carrier (16), and battery carrier (16) lower end is equipped with battery (17), installing frame mouth is provided with camera head, L-type chromatography cylinder (8) protrudes can making by uv-transmitting quartz plate of cavity for beneath end band has, its upper port is frosting, L-type chromatography cylinder (8) upper end is equipped with chromatoplate combination unit, the structure of chromatoplate combination unit is: comprise main folder block (14), sub-folder block (13), limited block (12), constant current block (10), the wet rod of control (15) and chromatoplate (9), main folder block (14) both sides, lower end are respectively equipped with two fixed blocks, distance between two pieces is identical with L-type chromatography cylinder (8) interior upper port, main folder block (14) side, upper end is provided with platform, platform side is vertically provided with the through hole installing the wet rod (15) of control, main folder block (14) main body side is provided with the trough with straight angle installing sub-folder block (13), described sub-folder block (13) is the middle strip cuboid with ramped recesses groove, described limited block (12) is the arc block with interior dog, described constant current block (10) is the rectangular block that 2000 order quartz ceramic for filtration are made, the wet rod (15) of described control is the circular barred body that 2000 order quartz sand filtration plates are made, chromatoplate (9) upper end is attached to main folder block (14) side, constant current block (10) is installed, the trough with straight angle of main folder block (14) other end is installed sub-folder block (13), two limited blocks (12) are pacified into by the intermediate recess groove of main folder block (14) and sub-folder block (13) and are separately clamped to two ends spacing, by chromatoplate (9), main folder block (14), constant current block (10), sub-folder block (13) is fixed together jointly, the wet rod (15) of control is inserted in the hole of main folder block (14), chromatography cylinder combination unit is inserted in L-type chromatography cylinder (8), the frosting of chromatography cylinder (8) upper end scribbles vaseline oil, upper end covers cylinder cap (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510255042.4A CN104792919B (en) | 2015-05-19 | 2015-05-19 | Constant current thin layer chromatograph |
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| CN201510255042.4A CN104792919B (en) | 2015-05-19 | 2015-05-19 | Constant current thin layer chromatograph |
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| CN104792919A true CN104792919A (en) | 2015-07-22 |
| CN104792919B CN104792919B (en) | 2016-06-15 |
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| CN201510255042.4A Expired - Fee Related CN104792919B (en) | 2015-05-19 | 2015-05-19 | Constant current thin layer chromatograph |
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Citations (10)
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| GB929578A (en) * | 1960-07-05 | 1963-06-26 | Shandon Scient Company Ltd | Improvements in paper-chromatography apparatus |
| US3629098A (en) * | 1968-09-27 | 1971-12-21 | Toyo Roshi Co Ltd | Continuous flow, preparative thin-layer chromatograph |
| US4865729A (en) * | 1985-11-04 | 1989-09-12 | Sepragen Corporation | Radial thin layer chromatography |
| JPH045566A (en) * | 1990-04-20 | 1992-01-09 | Shimadzu Corp | Horizontal developing method and transfer method for thin layer chromatoplate |
| EP0435990B1 (en) * | 1989-07-13 | 1994-04-20 | POSTAIRE, Eric | Device and installation of thin film chromatography, with improved development |
| CN2781369Y (en) * | 2005-04-26 | 2006-05-17 | 刘吉成 | Thin-layer chromatography program temp. control instrument |
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| CN201654007U (en) * | 2010-03-19 | 2010-11-24 | 齐齐哈尔医学院 | Thin-layer chromatography analyzer |
| CN104165963A (en) * | 2013-05-20 | 2014-11-26 | 四川逢春制药有限公司 | Thin-layer viewing device |
| CN204044122U (en) * | 2014-08-30 | 2014-12-24 | 湖北省农业科学院农业质量标准与检测技术研究所 | A kind of polycomponent portable detection equipment based on thin-layer chromatography |
-
2015
- 2015-05-19 CN CN201510255042.4A patent/CN104792919B/en not_active Expired - Fee Related
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB929578A (en) * | 1960-07-05 | 1963-06-26 | Shandon Scient Company Ltd | Improvements in paper-chromatography apparatus |
| US3629098A (en) * | 1968-09-27 | 1971-12-21 | Toyo Roshi Co Ltd | Continuous flow, preparative thin-layer chromatograph |
| US4865729A (en) * | 1985-11-04 | 1989-09-12 | Sepragen Corporation | Radial thin layer chromatography |
| EP0435990B1 (en) * | 1989-07-13 | 1994-04-20 | POSTAIRE, Eric | Device and installation of thin film chromatography, with improved development |
| JPH045566A (en) * | 1990-04-20 | 1992-01-09 | Shimadzu Corp | Horizontal developing method and transfer method for thin layer chromatoplate |
| CN2781369Y (en) * | 2005-04-26 | 2006-05-17 | 刘吉成 | Thin-layer chromatography program temp. control instrument |
| CN201654008U (en) * | 2010-03-19 | 2010-11-24 | 齐齐哈尔医学院 | Multifunctional thin-layer chromatography temperature and moisture controller |
| CN201654007U (en) * | 2010-03-19 | 2010-11-24 | 齐齐哈尔医学院 | Thin-layer chromatography analyzer |
| CN104165963A (en) * | 2013-05-20 | 2014-11-26 | 四川逢春制药有限公司 | Thin-layer viewing device |
| CN204044122U (en) * | 2014-08-30 | 2014-12-24 | 湖北省农业科学院农业质量标准与检测技术研究所 | A kind of polycomponent portable detection equipment based on thin-layer chromatography |
Non-Patent Citations (2)
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| CHENG-CHONG LI 等: "Hydroxysafflor yellow A induces apoptosis in activated hepatic stellate cells through ERK1/2 pathway in vitro", 《EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES》, vol. 46, 16 March 2012 (2012-03-16), pages 397 - 404, XP028489143, DOI: doi:10.1016/j.ejps.2012.03.003 * |
| 张宏莲 等: "TLC － IＲ 法检测降糖中药及保健品中掺入2种双胍类药物", 《药物分析杂志》, vol. 33, no. 12, 31 December 2013 (2013-12-31), pages 2061 - 2064 * |
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| CN104792919B (en) | 2016-06-15 |
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