CN104788208A - Chelated earthworm amino acid water-soluble micronutrient fertilizer and preparation method thereof - Google Patents
Chelated earthworm amino acid water-soluble micronutrient fertilizer and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of water-soluble fertilizer processing, and in particular relates to a chelated earthworm amino acid water-soluble micronutrient fertilizer and a preparation method thereof. The chelated earthworm amino acid water-soluble micronutrient fertilizer is characterized by consisting of the following components in parts by mass: 1-3 parts of citric acid, 50-60 parts of an earthworm amino acid chelation solution, 7-27 parts of ferrous sulfate, 10-27 parts of zinc sulfate, 7-27 parts of manganese sulfate and 2-12 parts of monopotassium phosphate. Due to the adoption of the chelated earthworm amino acid water-soluble micronutrient fertilizer, the micro-element nutrition deficiency disease of plants can be treated, the earthworm amino acid chelation solution is used as the liquid phase, the earthworm amino acid chelation solution contains 18 earthworm amino acids, through chelation reaction of the amino acid chelation solution and microelements like zinc, ferrum and manganese, the activity of enzyme inside plant bodies can be rapidly activated through the effective components, absorption of plants on macroelements like nitrogen, phosphorus and potassium can be accelerated, the utilization rate of the fertilizer can be increased, and conversion of carbohydrate inside plant bodies can be promoted, the yield can be increased, the content of chlorophyll of leaves can be increased, the cold resistance and the salt resistance can be improved, and the disease resistance and the adverse resistance of crops can be improved.
Description
Technical field
The invention belongs to water-soluble fertilizer manufacture field, particularly relate to water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid and preparation method thereof.
Background technology
Plant normal growth grow required for nutritive element have essential element and beneficial element point, have again dividing of macroelement and trace element in essential element.Essential element refer to plant normal growth grow must and the plant nutrient that can not replace with other elements.According to plant requirement number, essential element is divided into again required macroelement and essential trace element.Carbon (C), hydrogen (H), oxygen (O), nitrogen (N), phosphorus (P), sulphur (S), potassium (K), magnesium (Mg), calcium (Ca), silicon (Si) must be had by macroelement; Essential trace element has iron (Fe), manganese (Mn), zinc (Zn), copper (Cu), boron (B), molybdenum (Mo), chlorine (Cl), sodium (Na), nickel (Ni).
Nitrogen is not only plant materials internal protein, nucleic acid and chlorophyllous important component part, and is the integral part of multiple enzyme in plant materials.Meanwhile, all nitrogen is contained in some VITAMIN in plant materials and alkaloid.In protein, the average content of nitrogen is 16-18%, and protein forms plasmic base substance.All lived organisms be all be in protein continuous composition and decomposition among, if there is no nitrogen, would not protein be had, also just there is no life.Nitrogen is also the integral part of plant materials inner chlorophyll, and there is close relationship rich the lacking of nitrogen with the content of blade Determination of Chlorophyll, if green plants lacks nitrogen, can affect chlorophyllous formation, photosynthesis just can not carry out smoothly.Nitrogen supplying is sufficient, and plant can synthesize more chlorophyll.Symptom during general crop shortage nitrogen is: yellow from inferior leads, and gradually to top expansion, the root system of crop is whiter and elongated than the root system look of normal growth, but root amount reduces.
Phosphorus is the composition (as nucleic acid, phosphatide, adenosine triphosphate etc.) of many important organic compounds in plant materials, and physiology, the biological process in many ways in involved in plant body, to plant grow and metabolism all plays an important role.Nucleic acid and protein are protoplasma, nucleus and chromosomal important component, play an important role in the vital movement and heritable variation of plant.The formation of cell fission and neologism all can't do without them.Supply normal phosphorus nutrition, cell fission and propagation can be accelerated, enhancing development, and be conducive to the hereditary property keeping improved seeds.Particularly the fertility of crop is early stage, sufficient phosphorus nutrition to promote crop grow and precocious, good quality and high output plays an important role, otherwise growth is suppressed, and root system development is bad, even and if this impact a large amount of supply later be also difficult to make up completely.In nitrogen metabolism, phosphorus is also important, if phosphorus is not enough, will affect the synthesis of protein, time serious, protein also can decompose, thus affects the eubolism of nitrogen, so single nitrogen fertilizer application effect is bad when scarce phosphorus.If phosphorus supply is not enough, cell fission can be made to be obstructed, cessation of growth cessation; Root system development is bad, and blade is narrow, and leaf look dark green, becomes red-purple time serious.A host of facts show, sufficient phosphorus nutrition can improve the drought resisting of plant, the ability of cold-resistant, disease-resistant, resistant to lodging and acid and alkali-resistance, growing of plant can be promoted, promote the time of bud differentiation and shortening bud differentiation, thus can impel crop early flowering, maturation.
Potassium also has important effect to growing of plant, but it does not participate in formation biomacromolecule directly as nitrogen, phosphorus.Its Main Function is, when appropriate potassium exists, the enzyme of plant just can give full play to its effect.Potassium can promote photosynthesis.Data is had to show to contain the high blade of potassium than containing the low blade many conversions luminous energy 50%-70% of potassium.Thus, under the condition that illumination is bad, the effect of potash fertilizer is just more remarkable.In addition potassium can also promote the metabolism of carbohydrate, promotes the metabolism of nitrogen, makes plant utilize moisture cost-effectively and improve the resistance of plant.Because potassium can promote the synthesis of Mierocrystalline cellulose and xylogen, thus make plant stem sturdy, lodging tolerance is strengthened.In addition, because building-up process is strengthened, starch, protein content are increased, and reduces monose, the content of total free aminoacids etc., decrease the nutrient of causal organism.Therefore, when potassium is sufficient, the resistance against diseases of plant greatly strengthens.Such as, when potassium is sufficient, rice sheath blight disease, bacterial leaf-blight, rice blast, pestalotiopsis funerea and corn stalk rot disease can be alleviated, the harm of northern and southern leaf blight.Potassium can improve plant to the suffertibility of poor environment and the resistivities to sick worm, lodging such as arid, low temperature, salt damagies.Soil lacks the symptom of potassium: first turn to be yellow from the tip and edge of Lao Ye, and gradually wither, speckle appears in blade face, and then withered or in shrivelled coke-like, the mesophyll between last vein also dries up, and occurs brown spot and patch on blade face.
The content of iron in plant is few, is generally the some thousandths of of dry matter.Iron is that formation chlorophyll is necessary, just produces chlorosis during iron deficiency, and leaf, in faint yellow, is even white.The respiration of cell also participated in by iron, and in cellular respiration process, it is the composition of some enzymes.As can be seen here, iron plays an important role to respiration with for the process of begging for.The mobility root of iron in plant materials is little, and the iron in old leaf can not shift in cambium, and thus it can not be utilized once again.Therefore, during iron deficiency, lower blade often can keep green, and tender leaf presents chlorosis.Iron deficiency symptoms: during iron deficiency, lower blade can keep green, and tender leaf presents chlorosis.It is generally acknowledged that the imbalance of intermetallic in plant (such as Mo, Cu, Mn) easily causes iron deficiency.Other cause the reason of iron deficiency to have: (1) soil phosphorus is too much, and (2) soil pH is high, lime is many, cool and synthesis result that hydrocarbonate content is high.
The physiological action of manganese to plant is many-sided, and it is relevant with the activity of many enzymes.It is composition and the activator of multiple enzyme, can promote the metabolism of carbohydrate and the metabolism of nitrogen, have substantial connection with crop growth and output.The photosynthesis (photosynthetic oxygen evolution) of manganese and green plants, respiration and nitrate reduction have close relationship, and during manganese deficiency, photosynthesis of plant is obviously suppressed.Manganese can accelerate to sprout and maturation, increases the validity of phosphorus and calcium.Manganese deficiency symptom: first manganese deficiency symptom appears on spire, shows as yellow between vein, sometimes occurs a series of chocolate spot.The most often occur in alkaline pH soil at high organic-matter soil and the lower neutrality of Fe content.Rice leaf (water planting) vein of manganese deficiency is interrupted chlorosis, and occur brown speckle, time serious, spot is linked to be strip, is expanded into patch.
Zinc is the component of some enzyme of plant.Zinc is also promote that some metabolic reactions are required.Zinc generates for chlorophyll and forms carbohydrate and is absolutely necessary., except leaf chlorosis, often there is leaflet and phenomenon of clustering in shoot tip in zinc deficiency symptom: fruit tree lacks zinc all has finding at China's the South and the North.Be called " little leaf disease ".Time serious, branch is dead, production declining.Common in the north have apple tree and peach to lack zinc, and southern citrus to lack zinc phenomenon more general.In addition, also may there is scarce zinc in pears, Lee, apricot, cherry, grape etc.Deficiency Xn Rice Plants zinc shows as " rice contracting seedling ", and the white seedling of corn is also caused by scarce zinc sometimes.Soil contains zinc from every mu tens grams to several kilograms.Fine textured suil is usually high containing zinc than sandy soil.Along with soil pH raises, zinc reduces the validity of plant-growth.Lack chlorosis between zinc and the serious maize leaf blade arteries and veins lacking zinc and present yellow-green colour striped clearly, symptom mainly appears between middle arteries and veins and leaf margin, the serious appearance light brown strip necrotic tissue lacking zinc, and leaf margin and Zhong Mai both sides still keep green.
At present, according in People's Republic of China (PRC) agricultural industry criteria NY1428-2010 trace element water-soluble fertilizer, standard liquid phase is water, and plant is slow to the absorption rate of nitrogen, phosphorus, potassium macroelement, and the utilization ratio of fertilizer is low.
Summary of the invention
The present invention is directed to above-mentioned technical problem, provide high water-soluble trace element fertilizer of chelating type earthworm amino acid of a kind of utilization ratio of fertilizer and preparation method thereof.
The technical solution adopted in the present invention is:
The water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid, it is characterized in that, each components in mass portion array becomes: citric acid 1-3 part, earthworm chelating amino acids solution 50-60 part, ferrous sulfate 7-27 part, zinc sulfate 10-27 part, manganous sulfate 7-27 part, potassium primary phosphate 2-12 part.
Each component consists of by more high quality number: citric acid 1.5 parts, earthworm chelating amino acids solution 54 parts, 7 parts, ferrous sulfate, 25.5 parts, zinc sulfate, manganous sulfate 7 parts, potassium primary phosphate 5 parts.
Described ferrous sulfate selects the ferrous sulfate product of sulfur-bearing 18% iron content 31%, described zinc sulfate selects sulfur-bearing 11.6% containing the zinc sulfate product of zinc 23.8%, described manganous sulfate selects sulfur-bearing 21% containing the manganese sulfate product of manganese 31%, and described potassium primary phosphate selects phosphorous 52% containing the potassium dihydrogen phosphate product of potassium 33%.
A preparation method for the water-soluble trace element fertilizer of chelating type earthworm amino acid, is characterized in that, carries out in accordance with the following steps:
(1) preparation of earthworm chelating amino acids solution
A, fermentation: large flat No. two of victory becomes earthworm to be raw materials to select pure compatriot to like, screens out the various impurity of rinsing and drains water pouring, ferment at constant temperature 1-3 hour at 80-90 DEG C in fermentor tank;
B, self-dissolving: the requirement reaching described steps A just can self-dissolving, and what obtain is solution;
C, decomposition: the salt solution adding 100g/L-300g/L concentration in the solution obtained in described step B, in the homogenate of tissue homogenizer high speed after 5-15 minute, at 35-45 DEG C, heat the protein ingredient decomposed for 20-30 minute in earthworm body, obtain decomposition of the mixture;
D, filtration: the decomposition of the mixture separating device of described step C gained is carried out solid-liquid separation, filters and collect supernatant liquor;
E, Aspirate supernatant: by the supernatant liquor ultrafiltration 15-25 minute of step D gained, collect ultrafiltrated, then by ultrafiltrated ultrafiltration 15-25 minute again, leaves standstill 1-3 hour after concentrated effective ingredient liquid;
F, precipitation: collect the solution after leaving standstill in described step e and throw out;
G, chromatography: utilize tomography devices to carry out chromatography, obtain amino acid solution;
(2) preparation of fertilizer
(1) according to each component of above-mentioned consumption precise;
(2) earthworm chelating amino acids solution is put into reactor, be heated to 32-40 DEG C;
(3) in described step (2) gained solution, add citric acid and stir 10-15 minute;
(4) in described step (3) gained solution, add manganous sulfate and continue to stir 25-35 minute;
(5) in described step (4) gained solution, add ferric sulfate, zinc sulfate, continue to be heated to 50-55 DEG C, continuously stirring 0.5-1.5 hour, all dissolve also chelatropic reaction to solid material complete, stand-by;
(6) by described step (5) gained solution cooling 3-5 hour, be then filled in finished pot by 80 order stainless steel meshs and place 0.5-1.5 hour, inspection by sampling, qualifiedly to get product.
In described steps A, more excellent leavening temperature is 85 DEG C, more excellent fermentation time is 2 hours; In described step C, the more excellent concentration of salt solution is 200g/L, and the more excellent time of homogenate is 10 minutes, and preferably heat-up time is 40 DEG C, and preferably heat-up time is 25 minutes; In described step e, preferably the ultrafiltration time is 20 minutes to supernatant liquor, and the more excellent time of ultrafiltrated ultrafiltration is again 20 minutes, and after concentrated effective ingredient liquid, more excellent time of repose is 2 hours.
In described step (2), the more excellent Heating temperature of earthworm chelating amino acids solution is 36 DEG C.
In described step (3), preferably churning time is 12 minutes.
In described step (4), preferably churning time is 30 minutes.
In described step (5), preferably Heating temperature is 52 DEG C, and preferably churning time is 1 hour.
In described step (6), the more excellent time of solution cooling is 4 hours, and the more excellent time of placing in finished pot is 1 hour.
Beneficial effect of the present invention is:
Product of the present invention can improve the micro-element nutrition deficiency disease in plant, because NY1428-2010 trace element water-soluble fertilizer Plays liquid phase is water, and liquid phase uses earthworm chelating amino acids solution in the present invention, 18 kinds of earthworm amino acid are rich in earthworm chelating amino acids solution, by adding citric acid chelating liquid and zinc, iron, manganese microelement chelatropic reaction, its available nutrient can the activity of acute activation plant materials endoenzyme, accelerate plant to the absorption of N P and K macroelement, improve utilization rate of fertilizer, promote in plant materials carbohydrate-modifying, promote full seed, promote crop photosynthesis and protein synthesis, improve output, improve chlorophyll content in leaf blades, strengthen winter resistance and salt tolerance, strengthen the disease-resistant of crop, anti-adversity ability.
Embodiment
Embodiment 1
The water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid is prepared in accordance with the following steps:
(1) preparation of earthworm chelating amino acids solution
A, fermentation: large flat No. two of victory becomes earthworms to be raw materials to select pure compatriot to like, screens out the various impurity of rinsing and drains water pouring, ferment at constant temperature 3 hours under 80 degree in fermentor tank;
B, self-dissolving: the requirement reaching steps A just can self-dissolving, and what obtain is solution;
C, decomposition: the salt solution adding 100g/L concentration in solution obtained in stepb, in the homogenate of tissue homogenizer high speed after 8 minutes, heat the protein ingredient decomposed for 20 minutes in earthworm body, obtain decomposition of the mixture at 40 DEG C;
D, filtration: the decomposition of the mixture separating device of step C gained is carried out solid-liquid separation, filters and collect supernatant liquor;
E, Aspirate supernatant: by the supernatant liquor ultrafiltration 20 minutes of step D gained, collect ultrafiltrated, then by ultrafiltrated ultrafiltration 20 minutes again, leaves standstill 2 hours after concentrated effective ingredient liquid;
F, precipitation: collect the solution after leaving standstill in step e and throw out;
G, chromatography: utilize tomography devices to carry out chromatography, obtain amino acid solution;
(2) preparation of fertilizer
(1) according to each component of mass fraction precise: citric acid 3 parts, earthworm chelating amino acids solution 55 parts, 12 parts, ferrous sulfate, 15 parts, zinc sulfate, manganous sulfate 7 parts, potassium primary phosphate 8 parts, ferrous sulfate selects the ferrous sulfate product of sulfur-bearing 18% iron content 31%, zinc sulfate selects sulfur-bearing 11.6% containing the zinc sulfate product of zinc 23.8%, manganous sulfate selects sulfur-bearing 21% containing the manganese sulfate product of manganese 31%, and potassium primary phosphate selects phosphorous 52% containing the potassium dihydrogen phosphate product of potassium 33%;
(2) earthworm chelating amino acids solution is put into reactor, be heated to 32 DEG C;
(3) in step (2) gained solution, add citric acid and stir 12 minutes;
(4) in step (3) gained solution, add manganous sulfate and continue stirring 30 minutes;
(5) in step (4) gained solution, add ferric sulfate, zinc sulfate, continue to be heated to 52 DEG C, continuously stirring 0.5 hour, all dissolve also chelatropic reaction to solid material complete, stand-by;
(6) step (5) gained solution is cooled 4 hours, be then filled in finished pot by 80 order stainless steel meshs and place 1 hour, inspection by sampling, qualifiedly gets product.
Embodiment 2
The water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid is prepared in accordance with the following steps:
(1) preparation of earthworm chelating amino acids solution
A, fermentation: large flat No. two of victory becomes earthworms to be raw materials to select pure compatriot to like, screens out the various impurity of rinsing and drains water pouring, ferment at constant temperature 2 hours under 85 degree in fermentor tank;
B, self-dissolving: the requirement reaching steps A just can self-dissolving, and what obtain is solution;
C, decomposition: the salt solution adding 150g/L concentration in solution obtained in stepb, in the homogenate of tissue homogenizer high speed after 15 minutes, heat the protein ingredient decomposed for 20 minutes in earthworm body, obtain decomposition of the mixture at 40 DEG C;
D, filtration: the decomposition of the mixture separating device of step C gained is carried out solid-liquid separation, filters and collect supernatant liquor;
E, Aspirate supernatant: by the supernatant liquor ultrafiltration 15 minutes of step D gained, collect ultrafiltrated, then by ultrafiltrated ultrafiltration 15 minutes again, leaves standstill 3 hours after concentrated effective ingredient liquid;
F, precipitation: collect the solution after leaving standstill in step e and throw out;
G, chromatography: utilize tomography devices to carry out chromatography, obtain amino acid solution;
(2) preparation of fertilizer
(1) according to each component of mass fraction precise: citric acid 1 part, earthworm chelating amino acids solution 50 parts, 14 parts, ferrous sulfate, 18 parts, zinc sulfate, manganous sulfate 15 parts, potassium primary phosphate 2 parts, ferrous sulfate selects the ferrous sulfate product of sulfur-bearing 18% iron content 31%, zinc sulfate selects sulfur-bearing 11.6% containing the zinc sulfate product of zinc 23.8%, manganous sulfate selects sulfur-bearing 21% containing the manganese sulfate product of manganese 31%, and potassium primary phosphate selects phosphorous 52% containing the potassium dihydrogen phosphate product of potassium 33%;
(2) earthworm chelating amino acids solution is put into reactor, be heated to 40 DEG C;
(3) in step (2) gained solution, add citric acid and stir 10 minutes;
(4) in step (3) gained solution, add manganous sulfate and continue stirring 28 minutes;
(5) in step (4) gained solution, add ferric sulfate, zinc sulfate, continue to be heated to 55 DEG C, continuously stirring 0.5 hour, all dissolve also chelatropic reaction to solid material complete, stand-by;
(6) step (5) gained solution is cooled 4 hours, be then filled in finished pot by 80 order stainless steel meshs and place 1 hour, inspection by sampling, qualifiedly gets product.
Embodiment 3
The water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid is prepared in accordance with the following steps:
(1) preparation of earthworm chelating amino acids solution
A, fermentation: large flat No. two of victory becomes earthworms to be raw materials to select pure compatriot to like, screens out the various impurity of rinsing and drains water pouring, ferment at constant temperature 2 hours under 85 degree in fermentor tank;
B, self-dissolving: the requirement reaching steps A just can self-dissolving, and what obtain is solution;
C, decomposition: the salt solution adding 200g/L concentration in solution obtained in stepb, in the homogenate of tissue homogenizer high speed after 10 minutes, heat the protein ingredient decomposed for 25 minutes in earthworm body, obtain decomposition of the mixture at 40 DEG C;
D, filtration: the decomposition of the mixture separating device of step C gained is carried out solid-liquid separation, filters and collect supernatant liquor;
E, Aspirate supernatant: by the supernatant liquor ultrafiltration 20 minutes of step D gained, collect ultrafiltrated, then by ultrafiltrated ultrafiltration 20 minutes again, leaves standstill 2 hours after concentrated effective ingredient liquid;
F, precipitation: collect the solution after leaving standstill in step e and throw out;
G, chromatography: utilize tomography devices to carry out chromatography, obtain amino acid solution;
(2) preparation of fertilizer
(1) according to each component of mass fraction precise: citric acid 1.5 parts, earthworm chelating amino acids solution 54 parts, 7 parts, ferrous sulfate, 25.5 parts, zinc sulfate, manganous sulfate 7 parts, potassium primary phosphate 5 parts, ferrous sulfate selects the ferrous sulfate product of sulfur-bearing 18% iron content 31%, zinc sulfate selects sulfur-bearing 11.6% containing the zinc sulfate product of zinc 23.8%, manganous sulfate selects sulfur-bearing 21% containing the manganese sulfate product of manganese 31%, and potassium primary phosphate selects phosphorous 52% containing the potassium dihydrogen phosphate product of potassium 33%;
(2) earthworm chelating amino acids solution is put into reactor, be heated to 36 DEG C;
(3) in step (2) gained solution, add citric acid and stir 12 minutes;
(4) in step (3) gained solution, add manganous sulfate and continue stirring 30 minutes;
(5) in step (4) gained solution, add ferric sulfate, zinc sulfate, continue to be heated to 52 DEG C, continuously stirring 1 hour, all dissolve also chelatropic reaction to solid material complete, stand-by;
(6) step (5) gained solution is cooled 4 hours, be then filled in finished pot by 80 order stainless steel meshs and place 1 hour, inspection by sampling, qualifiedly gets product.
Embodiment 4
The water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid is prepared in accordance with the following steps:
(1) preparation of earthworm chelating amino acids solution
A, fermentation: large flat No. two of victory becomes earthworms to be raw materials to select pure compatriot to like, screens out the various impurity of rinsing and drains water pouring, ferment at constant temperature 1.5 hours under 88 degree in fermentor tank;
B, self-dissolving: the requirement reaching steps A just can self-dissolving, and what obtain is solution;
C, decomposition: the salt solution adding 250g/L concentration in solution obtained in stepb, in the homogenate of tissue homogenizer high speed after 15 minutes, heat the protein ingredient decomposed for 25 minutes in earthworm body, obtain decomposition of the mixture at 42 DEG C;
D, filtration: the decomposition of the mixture separating device of step C gained is carried out solid-liquid separation, filters and collect supernatant liquor;
E, Aspirate supernatant: by the supernatant liquor ultrafiltration 25 minutes of step D gained, collect ultrafiltrated, then by ultrafiltrated ultrafiltration 25 minutes again, leaves standstill 1 hour after concentrated effective ingredient liquid;
F, precipitation: collect the solution after leaving standstill in step e and throw out;
G, chromatography: utilize tomography devices to carry out chromatography, obtain amino acid solution;
(2) preparation of fertilizer
(1) according to each component of mass fraction precise: citric acid 3 parts, earthworm chelating amino acids solution 60 parts, 8 parts, ferrous sulfate, 20 parts, zinc sulfate, manganous sulfate 7 parts, potassium primary phosphate 2 parts, ferrous sulfate selects the ferrous sulfate product of sulfur-bearing 18% iron content 31%, zinc sulfate selects sulfur-bearing 11.6% containing the zinc sulfate product of zinc 23.8%, manganous sulfate selects sulfur-bearing 21% containing the manganese sulfate product of manganese 31%, and potassium primary phosphate selects phosphorous 52% containing the potassium dihydrogen phosphate product of potassium 33%;
(2) earthworm chelating amino acids solution is put into reactor, be heated to 40 DEG C;
(3) in step (2) gained solution, add citric acid and stir 14 minutes;
(4) in step (3) gained solution, add manganous sulfate and continue stirring 28 minutes;
(5) in step (4) gained solution, add ferric sulfate, zinc sulfate, continue to be heated to 53 DEG C, continuously stirring 0.8 hour, all dissolve also chelatropic reaction to solid material complete, stand-by;
(6) step (5) gained solution is cooled 3 hours, be then filled in finished pot by 80 order stainless steel meshs and place 1.2 hours, inspection by sampling, qualifiedly gets product.
Embodiment 5
The water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid is prepared in accordance with the following steps:
(1) preparation of earthworm chelating amino acids solution
A, fermentation: large flat No. two of victory becomes earthworms to be raw materials to select pure compatriot to like, screens out the various impurity of rinsing and drains water pouring, ferment at constant temperature 3 hours under 83 degree in fermentor tank;
B, self-dissolving: the requirement reaching steps A just can self-dissolving, and what obtain is solution;
C, decomposition: the salt solution adding 280g/L concentration in solution obtained in stepb, in the homogenate of tissue homogenizer high speed after 10 minutes, heat the protein ingredient decomposed for 20 minutes in earthworm body, obtain decomposition of the mixture at 38 DEG C;
D, filtration: the decomposition of the mixture separating device of step C gained is carried out solid-liquid separation, filters and collect supernatant liquor;
E, Aspirate supernatant: by the supernatant liquor ultrafiltration 20 minutes of step D gained, collect ultrafiltrated, then by ultrafiltrated ultrafiltration 20 minutes again, leaves standstill 3 hours after concentrated effective ingredient liquid;
F, precipitation: collect the solution after leaving standstill in step e and throw out;
G, chromatography: utilize tomography devices to carry out chromatography, obtain amino acid solution;
(2) preparation of fertilizer
(1) according to each component of mass fraction precise: citric acid 2 parts, earthworm chelating amino acids solution 52 parts, 10 parts, ferrous sulfate, 15 parts, zinc sulfate, manganous sulfate 15 parts, potassium primary phosphate 6 parts, ferrous sulfate selects the ferrous sulfate product of sulfur-bearing 18% iron content 31%, zinc sulfate selects sulfur-bearing 11.6% containing the zinc sulfate product of zinc 23.8%, manganous sulfate selects sulfur-bearing 21% containing the manganese sulfate product of manganese 31%, and potassium primary phosphate selects phosphorous 52% containing the potassium dihydrogen phosphate product of potassium 33%;
(2) earthworm chelating amino acids solution is put into reactor, be heated to 34 DEG C;
(3) in step (2) gained solution, add citric acid and stir 14 minutes;
(4) in step (3) gained solution, add manganous sulfate and continue stirring 25 minutes;
(5) in step (4) gained solution, add ferric sulfate, zinc sulfate, continue to be heated to 53 DEG C, continuously stirring 0.5 hour, all dissolve also chelatropic reaction to solid material complete, stand-by;
(6) step (5) gained solution is cooled 5 hours, be then filled in finished pot by 80 order stainless steel meshs and place 1.5 hours, inspection by sampling, qualifiedly gets product.
Experiment test
1, earthworm chelating amino acids solution Middle nutrition composition measurement experiment
According to the method that GB/T 5009,124-2003 specify, the aminoacids content in the earthworm chelating amino acids solution in the present invention is detected, wherein, tryptophane is hydrolyzed with 4.2molNaOH, Gelucystine is measured with the process of performic oxidation method, all the other amino acid measure with 6molHCl hydrolysis, and concrete detected result is as shown in table 1; Detect other nutritive ingredient in earthworm chelating amino acids solution, concrete detected result is as shown in table 2.
Table 1 aminoacids content detected result
| Amino acid abbreviations | Amino acid | Detect data |
| ASP | Aspartic acid | 0.0109g/100g |
| THR | Threonine | 0.0023g/100g |
| SER | Serine | 0.0031g/100g |
| GLU | L-glutamic acid | 0.0070g/100g |
| GLY | Glycine | 0.0085g/100g |
| ALA | L-Ala | 0.0379g/100g |
| VAL | α-amino-isovaleric acid | 0.0049g/100g |
| MET | Methionine(Met) | 0.0001g/100g |
| ILE | Isoleucine | 0.0022g/100g |
| LEU | Leucine | 0.0082g/100g |
| TYR | Tyrosine | 0.0012g/100g |
| PHE | Phenylalanine | 0.0006g/100g |
| LYS | Methionin | 0.0063g/100g |
| HIS | Histidine | 0.0005g/100g |
| ARG | Arginine | 0.0017g/100g |
| PRO | Proline(Pro) | 0.0022g/100g |
| TRP | Tryptophane | 0.0001g/100g |
| CYS | Gelucystine | 0.0019g/100g |
| Summation | 0.0995/100g |
Other nutrient composition content detected result of table 2
From data in table 1, table 2, in earthworm chelating amino acids solution, be rich in amino acid in 18, and containing other nutritive substance multiple, nutritious.
2, stimulation ratio determination experiment
Select physical features smooth, the soil texture is even, soil alkaline moderate, the complete intact soil of drip irrigation facility, select watermelon, tomato, paddy rice three kinds of crops carry out field experiment, 18 groups of crop treatment zones are established in each embodiment product field altogether: 3 groups of watermelon common fertilizer districts, 3 groups of water-soluble trace element fertilizer application zone of watermelon chelating type earthworm amino acid, 3 groups of tomato common fertilizer districts, 3 groups of water-soluble trace element fertilizer application zone of tomato chelating type earthworm amino acid, 3 groups of paddy rice common fertilizer districts, 3 groups of water-soluble trace element fertilizer application zone of paddy rice chelating type earthworm amino acid, the soil of the identical material of the same area is all selected in various crop treatment zones, often kind of crop all selects same breed.
Wherein 3 groups of watermelon common fertilizer districts, 3 groups of tomato common fertilizer districts, 3 groups of paddy rice common fertilizer districts use the common fertilizer of optimum amount on time, other 3 groups of water-soluble trace element fertilizer application zone of watermelon chelating type earthworm amino acid, 3 groups of water-soluble trace element fertilizer application zone of tomato chelating type earthworm amino acid, the water-soluble trace element fertilizer of chelating type earthworm amino acid of optimum amount is used in 3 groups of paddy rice chelating type earthworm amino acid water-soluble trace element fertilizer application zone on time, chelating type earthworm amino acid water-soluble trace element fertilizer dilution 300-400 doubly rear foliage-spray, common fertilizer district output, the numerical value of chelating type earthworm amino acid water-soluble trace element fertilizer application zone output all gets the mean value of same district, specific experiment the results are shown in Table 3.
Wherein stimulation ratio=(trace element fertilizer application zone output-common fertilizer district output)/common fertilizer district output * 100%
Table 3 each district output particular sheet
From data in table 3, the stimulation ratio of watermelon microelement Term Fertilization district output is up to 20.3%, the stimulation ratio of tomato trace element fertilizer application zone output up to 19.6%, the stimulation ratio of paddy rice trace element fertilizer application zone output up to 25.4%, obvious effect of increasing production.
3, carry out the mensuration of fertilizer utilization increase rate about the differential technique printed and distributed in the letter of " Term Fertilization effect measuring method (trying) " according to No. (2008) 315, agrotechnical clay fertilizer water letter, concrete measurement result is as shown in table 4.
Table 4 fertilizer utilization increase rate particular sheet
From data in table 4, watermelon fertilizer utilization increase rate is up to 32%, and tomato fertilizer utilization increase rate is up to 23.2%, and paddy rice fertilizer utilization increase rate is up to 24.3%, and utilization rate of fertilizer improves significantly.
4, micro-element nutrition deficiency disease improves experiment
(1) iron deficiency symptoms improves experiment
Choose tomato and there are 18 crop treatment zones of iron deficiency symptoms as experimental subjects: 3 tomato space management districts, 15 tomato experimental group treatment zones, 18 crops of crop treatment zone all in the same area and in each treatment zone are no less than 1000 strains, conventional NPK fertilizers is applied in space management district, the water-soluble trace element fertilizer of chelating type earthworm amino acid in embodiment 1-5 is applied in experimental group treatment zone, the applying quantity of each embodiment in experimental group treatment zone is 3, space management district, other growth conditions of experimental group treatment zone is all identical, the applying consumption of embodiment 1-5 is 1.5 kgs/acre, chelating type earthworm amino acid water-soluble trace element fertilizer dilution 300-400 doubly rear foliage-spray, it is as shown in table 5 that concrete symptom improves situation.
Table 5 tomato symptom improves particular sheet
| Embodiment | Symptom is improved significantly (%) | Symptom is improved obviously (%) | Efficient (%) |
| 1 | 71 | 9 | 80 |
| 2 | 74 | 12 | 86 |
| 3 | 75 | 13 | 88 |
| 4 | 73 | 11 | 84 |
| 5 | 70 | 13 | 83 |
Wherein, symptom is improved significantly finger spire and is recovered green, and the lark spot of blade base disappears, shrivelled downright bad transference cure between arteries and veins; Symptom is improved obviously finger spire and is substantially recovered green, and the lark spot of blade base disappears substantially, and between arteries and veins, shrivelled downright bad symptom disappears substantially; Efficient=symptom is improved significantly+symptom and is improved obviously.
From data in table 5, iron deficiency symptoms improves the efficient up to 88% of experiment, and symptom is improved significantly.
(2) zinc deficiency symptom improves experiment
Choose paddy rice and there are 18 crop treatment zones of zinc deficiency symptom as experimental subjects: 3 paddy rice space management districts, 15 paddy rice experimental group treatment zones, 18 crops of crop treatment zone all in the same area and in each treatment zone are no less than 1000 strains, conventional NPK fertilizers is applied in space management district, the water-soluble trace element fertilizer of chelating type earthworm amino acid in embodiment 1-5 is applied in experimental group treatment zone, the applying quantity of each embodiment in experimental group treatment zone is 3, space management district, other growth conditions of experimental group treatment zone is all identical, the applying consumption of embodiment 1-5 is 2 kgs/acre, chelating type earthworm amino acid water-soluble trace element fertilizer dilution 300-400 doubly rear foliage-spray, it is as shown in table 6 that concrete symptom improves situation.
Table 6 paddy rice symptom improves particular sheet
| Embodiment | Symptom is improved significantly (%) | Symptom is improved obviously (%) | Efficient (%) |
| 1 | 74 | 9 | 83 |
| 2 | 76 | 12 | 88 |
| 3 | 78 | 12 | 90 |
| 4 | 76 | 13 | 89 |
| 5 | 73 | 9 | 82 |
Wherein, symptom improvement significantly refers to that in young leaves, muscle recovers green, and Lao Ye brown spot disappears, and root system is strong, and plant height recovery is normal; Symptom is improved muscle in obvious young leaves and is substantially recovered green, and Lao Ye brown spot disappears substantially, and root system is strong, and plant height recovers normal substantially; Efficient=symptom is improved significantly+symptom and is improved obviously.
From data in table 6, iron deficiency symptoms improves the efficient up to 90% of experiment, and symptom is improved significantly.
(5) manganese deficiency symptom improves experiment
Choose soybean and there are 18 crop treatment zones of manganese deficiency symptom as experimental subjects: 3 soybean space management districts, 15 soybean experimental group treatment zones, 18 crops of crop treatment zone all in the same area and in each treatment zone are no less than 1000 strains, conventional NPK fertilizers is applied in space management district, the water-soluble trace element fertilizer of chelating type earthworm amino acid in embodiment 1-5 is applied in experimental group treatment zone, the applying quantity of each embodiment in experimental group treatment zone is 3, space management district, other growth conditions of experimental group treatment zone is all identical, the applying consumption of embodiment 1-5 is 2 kgs/acre, chelating type earthworm amino acid water-soluble trace element fertilizer dilution 300-400 doubly rear foliage-spray, it is as shown in table 7 that concrete symptom improves situation.
Table 7 soybean symptom improves particular sheet
| Embodiment | Symptom is improved significantly (%) | Symptom is improved obviously (%) | Efficient (%) |
| 1 | 73 | 12 | 85 |
| 2 | 75 | 9 | 84 |
| 3 | 77 | 15 | 92 |
| 4 | 76 | 12 | 88 |
| 5 | 79 | 10 | 89 |
Wherein, symptom is improved significantly finger young leaves and is recovered green, and greyish white or foxiness disappears; Symptom is improved obviously finger young leaves and is substantially recovered green, and greyish white or foxiness disappears substantially; Efficient=symptom is improved significantly+symptom and is improved obviously.
From data in table 7, iron deficiency symptoms improves the efficient up to 92% of experiment, and symptom is improved significantly.
In above-mentioned field experiment process, use in the region of the water-soluble trace element fertilizer of chelating type earthworm amino acid, crop kernel is full, and leaf green is tempting, and along with the raising of winter resistance and salt tolerance and enhancing that is disease-resistant, anti-adversity ability.
Above 5 embodiments of the present invention have been described in detail, but described content being only preferred embodiment of the present invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the present patent application scope change and improve, and all should still belong within patent covering scope of the present invention.
Claims (10)
1. the water-soluble trace element fertilizer of chelating type earthworm amino acid, is characterized in that, each components in mass portion array becomes: citric acid 1-3 part, earthworm chelating amino acids solution 50-60 part, ferrous sulfate 7-27 part, zinc sulfate 10-27 part, manganous sulfate 7-27 part, potassium primary phosphate 2-12 part.
2. the water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid according to claim 1, it is characterized in that, each component consists of by more high quality number: citric acid 1.5 parts, earthworm chelating amino acids solution 54 parts, 7 parts, ferrous sulfate, 25.5 parts, zinc sulfate, manganous sulfate 7 parts, potassium primary phosphate 5 parts.
3. water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid according to claim 1 and preparation method thereof, it is characterized in that, described ferrous sulfate selects the ferrous sulfate product of sulfur-bearing 18% iron content 31%, described zinc sulfate selects sulfur-bearing 11.6% containing the zinc sulfate product of zinc 23.8%, described manganous sulfate selects sulfur-bearing 21% containing the manganese sulfate product of manganese 31%, and described potassium primary phosphate selects phosphorous 52% containing the potassium dihydrogen phosphate product of potassium 33%.
4. a preparation method for the water-soluble trace element fertilizer of chelating type earthworm amino acid, is characterized in that, carries out in accordance with the following steps:
(1) preparation of earthworm chelating amino acids solution
A, fermentation: large flat No. two of victory becomes earthworm to be raw materials to select pure compatriot to like, screens out the various impurity of rinsing and drains water pouring, ferment at constant temperature 1-3 hour at 80-90 DEG C in fermentor tank;
B, self-dissolving: the requirement reaching described steps A just can self-dissolving, and what obtain is solution;
C, decomposition: the salt solution adding 100g/L-300g/L concentration in the solution obtained in described step B, in the homogenate of tissue homogenizer high speed after 5-15 minute, at 35-45 DEG C, heat the protein ingredient decomposed for 20-30 minute in earthworm body, obtain decomposition of the mixture;
D, filtration: the decomposition of the mixture separating device of described step C gained is carried out solid-liquid separation, filters and collect supernatant liquor;
E, Aspirate supernatant: by the supernatant liquor ultrafiltration 15-25 minute of step D gained, collect ultrafiltrated, then by ultrafiltrated ultrafiltration 15-25 minute again, leaves standstill 1-3 hour after concentrated effective ingredient liquid;
F, precipitation: collect the solution after leaving standstill in described step e and throw out;
G, chromatography: utilize tomography devices to carry out chromatography, obtain amino acid solution;
(2) preparation of fertilizer
(1) according to each component of above-mentioned consumption precise;
(2) earthworm chelating amino acids solution is put into reactor, be heated to 32-40 DEG C;
(3) in described step (2) gained solution, add citric acid and stir 10-15 minute;
(4) in described step (3) gained solution, add manganous sulfate and continue to stir 25-35 minute;
(5) in described step (4) gained solution, add ferric sulfate, zinc sulfate, continue to be heated to 50-55 DEG C, continuously stirring 0.5-1.5 hour, all dissolve also chelatropic reaction to solid material complete, stand-by;
(6) by described step (5) gained solution cooling 3-5 hour, be then filled in finished pot by 80 order stainless steel meshs and place 0.5-1.5 hour, inspection by sampling, qualifiedly to get product.
5. the preparation method of the water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid according to claim 4, is characterized in that, in described steps A, more excellent leavening temperature is 85 DEG C, more excellent fermentation time is 2 hours; In described step C, the more excellent concentration of salt solution is 200g/L, and the more excellent time of homogenate is 10 minutes, and preferably heat-up time is 40 DEG C, and preferably heat-up time is 25 minutes; In described step e, preferably the ultrafiltration time is 20 minutes to supernatant liquor, and the more excellent time of ultrafiltrated ultrafiltration is again 20 minutes, and after concentrated effective ingredient liquid, more excellent time of repose is 2 hours.
6. the preparation method of the water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid according to claim 4, is characterized in that, in described step (2), the more excellent Heating temperature of earthworm chelating amino acids solution is 36 DEG C.
7. the preparation method of the water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid according to claim 4, is characterized in that, in described step (3), preferably churning time is 12 minutes.
8. the preparation method of the water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid according to claim 4, is characterized in that, in described step (4), preferably churning time is 30 minutes.
9. the preparation method of the water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid according to claim 4, is characterized in that, in described step (5), preferably Heating temperature is 52 DEG C, and preferably churning time is 1 hour.
10. the preparation method of the water-soluble trace element fertilizer of a kind of chelating type earthworm amino acid according to claim 4, is characterized in that, in described step (6), the more excellent time of solution cooling is 4 hours, and the more excellent time of placing in finished pot is 1 hour.
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| CN105218263A (en) * | 2015-11-20 | 2016-01-06 | 安徽思福农业科技有限公司 | A kind of ammino acid liquid fertilizer |
| CN107935683A (en) * | 2017-11-22 | 2018-04-20 | 宿州市逢源生物科技有限公司 | A kind of production method of the liquid fertilizer based on earthworm enzymolysis composite aminoacid chelating |
| CN110495474A (en) * | 2019-09-29 | 2019-11-26 | 黄河三角洲京博化工研究院有限公司 | A kind of biostimulant and preparation method thereof |
| CN117049921A (en) * | 2023-09-11 | 2023-11-14 | 山东辰昕生物科技有限公司 | Preparation method of earthworm organic water-soluble fertilizer |
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Cited By (4)
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| CN107935683A (en) * | 2017-11-22 | 2018-04-20 | 宿州市逢源生物科技有限公司 | A kind of production method of the liquid fertilizer based on earthworm enzymolysis composite aminoacid chelating |
| CN110495474A (en) * | 2019-09-29 | 2019-11-26 | 黄河三角洲京博化工研究院有限公司 | A kind of biostimulant and preparation method thereof |
| CN117049921A (en) * | 2023-09-11 | 2023-11-14 | 山东辰昕生物科技有限公司 | Preparation method of earthworm organic water-soluble fertilizer |
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