CN104787902A - Method for adsorbing heavy metal in wastewater by using mold spore - Google Patents
Method for adsorbing heavy metal in wastewater by using mold spore Download PDFInfo
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- CN104787902A CN104787902A CN201510226403.2A CN201510226403A CN104787902A CN 104787902 A CN104787902 A CN 104787902A CN 201510226403 A CN201510226403 A CN 201510226403A CN 104787902 A CN104787902 A CN 104787902A
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Abstract
The invention discloses a method for adsorbing heavy metals in wastewater by using mold spore, and aims to solve the problem that a conventional mold mycelium pellet powder adsorbent is poor in effect and high in cost. The method comprises the following steps: I, inoculating mold spore to a solid culture medium, culturing at 30 DEG C, and subsequently cooling the mold spore which is naturally dropped off from atmogenic hyphae of mold; II, feeding the mold spore collected in the step I into wastewater with the heavy metal, thereby completing the operation. The method has the advantages that due to collection of the spore, an adsorbent preparation step can be simplified, the cost can be reduced, and meanwhile the heavy metal adsorption amount can be increased. The method can be applied to the field of adsorbents.
Description
Technical field
The present invention relates to a kind of method of adsorbing heavy metal in waste water.
Background technology
Traditional heavy metal removal technology: the traditional method of the removal for heavy metal has chemical settling, redox, ion-exchange, electrochemical treatment, filtration, reverse osmosis, solvent extraction and evaporation to reclaim.The method of various physics, chemistry is also the transfer of heavy metal ion, concentrated or existence form change, and heavy metal can not disappear, and total amount can not reduce, and this is that heavy metal wastewater thereby is different from other waste water part.In those early years, ion exchange method application is more extensive, but because resin cost is higher, there is certain restriction to original water quality, as useless Organic substance in water and oxide compound can make efficiency lost resin etc., so, the consequent is that chemical method, electrolytic process etc. engender and be applied, and the weak point of chemical method is that floor space is comparatively large, produces sludge containing heavy metal, mud does not obtain appropriate process, so there is secondary pollution problem.The weak point of electrolytic process is that power consumption is high, and when heavy metal content is higher, result of use is undesirable, and electrolytic process is usually used in the recovery of noble metal, and for the not high metal of recovery value, such as copper, that is just lost more than gain.Environmentalist proposes likely to utilize biological process removal heavy metal very early, and has carried out years of researches.What extensively adopt at present is all that strain inoculation is in liquid nutrient medium, under the culture condition of certain temperature and rotating speed, shake-flask culture goes out mycelium pellet, available sorbent material is just become again after washing, filter, dry, grind and sieving, although environmental protection, but the culture condition of its loaded down with trivial details operation steps, expensive substratum expense and harshness makes it be difficult to realize industrialization.
Summary of the invention
The object of the invention is to solve the problem that existing mould hyphaball powder sorbent adsorption effect is poor and cost is high, and a kind of method utilizing mould spores to adsorb heavy metal in waste water provided.
The present invention is a kind of utilizes the method that mould spores adsorbs heavy metal in waste water to prepare according to following steps:
One, mould spores is inoculated on solid medium, cultivates under the condition of 29 ~ 31 DEG C, then collect the mould spores that mould aerial hyphae comes off naturally;
Two, being added in the waste water containing heavy metal by the mould spores that step one is collected, is then stir under the condition of 10 ~ 40 DEG C in temperature, and absorption 1.5 ~ 2.5h, namely completes; Wherein mould is the aspergillus niger of bacterial strain numbers 98003; Mould spores is 1:(900 ~ 1100 with the mass volume ratio of the waste water containing heavy metal) g/mL.
Advantage of the present invention is that the collection of spore can simplify absorbent preparation step, improves heavy metal adsorption amount while cost-saving.Spore is that mould was bred by the aerial hyphae broken up in the solid medium surface growth later stage, can self falling after about 7 days, spore is the extremely strong hypopus of a kind of resistance, its resistance against physical effect that is chemical and medicine is very strong, even if so cell walls is also not easy to be destroyed in adsorption process, the diameter of spore is very little simultaneously, is approximately 2um, therefore has larger specific surface area.
Embodiment
Embodiment one: present embodiment is a kind of utilizes the method that mould spores adsorbs heavy metal in waste water to prepare according to following steps:
One, mould spores is inoculated on solid medium, cultivates under the condition of 29 ~ 31 DEG C, then collect the mould spores that mould aerial hyphae comes off naturally;
Two, being added in the waste water containing heavy metal by the mould spores that step one is collected, is then stir under the condition of 10 ~ 40 DEG C in temperature, and absorption 1.5 ~ 2.5h, namely completes; Mould spores is 1:(900 ~ 1100 with the mass volume ratio of the waste water containing heavy metal) g/mL.
Mould wherein in present embodiment is the aspergillus niger of bacterial strain numbers 98003, buys from Harbin Chun Rui Science and Technology Development Co., Ltd..
The advantage of present embodiment is that the collection of spore can simplify absorbent preparation step, improves heavy metal adsorption amount while cost-saving.
Embodiment two: present embodiment and embodiment one are the aspergillus niger (Aspergillus niger) of bacterial strain numbers 98003 unlike: described mould.Other are identical with embodiment one.
Embodiment three: present embodiment and embodiment one or two unlike: the solid medium described in step one is for improve Martin's substratum, PDA substratum or dry rice straw substratum.Other are identical with embodiment one or two.
Present embodiment improvement Martin substratum: peptone 5.0g, dipotassium hydrogen phosphate 1.0g, yeast extract powder 2.0g, magnesium sulfate 0.5g, glucose 20.0g, agar powder 15g, water 1000ml, ph6.4;
PDA substratum: 200g peeled potatoes, after poach 30min, distilled water supplies 1000ml, adds 20g glucose, agar powder 15g;
Dry rice straw substratum: milled 60 mesh sieves of dry rice straw, add clear water with mass volume ratio 1:1.
Embodiment four: one of present embodiment and embodiment one to three are unlike heavy metal copper described in step 2.Other steps are identical with one of embodiment one to three with parameter.
Embodiment five: one of present embodiment and embodiment one to four unlike stirring described in step 2 under temperature is the condition of 30 DEG C.Other steps are identical with one of embodiment one to four with parameter.
Embodiment six: one of present embodiment and embodiment one to five unlike: the stirring velocity described in step 2 is 0 ~ 300rmp.Other steps are identical with one of embodiment one to five with parameter.
Embodiment seven: one of present embodiment and embodiment one to six unlike: adsorption time is 2h.Other steps are identical with one of embodiment one to six with parameter.
By following verification experimental verification beneficial effect of the present invention:
Test 1, this test test group utilize the method that mould spores adsorbs heavy metal in waste water to prepare according to following steps:
One, mould spores is inoculated on improvement Martin substratum, cultivates 6 ~ 7 days under the condition of 30 DEG C, then collect the mould spores that mould aerial hyphae comes off naturally;
Two, the mould spores that step one is collected being added to copper content is in the waste water of 30mg/L, then in temperature is to stir under the condition of 30 DEG C, and stirring velocity is 100rmp, and absorption 2h, namely completes; The wherein concrete strain name of mould and Latin; The mass volume ratio of mould spores and copper-containing wastewater is 1:1000g/mL.
The adsorptive capacity of test group spore is 27.2mg/g.
It is in the waste water of 30mg/L that mould hyphaball powder sorbent is added to copper content by control group, then in temperature is to stir under the condition of 30 DEG C, and stirring velocity is 100rmp, and absorption 2h, namely completes; The wherein concrete strain name of mould and Latin; Mould hyphaball powder sorbent is 1:1000g/mL with the mass volume ratio containing heavy copper waste water.
Wherein the preparation method of mould hyphaball powder sorbent is: be inoculated into by mould in liquid nutrient medium, under 30 DEG C and rotating speed are the culture condition of 100rmp, shake-flask culture goes out mycelium pellet, more namely obtains mould hyphaball powder sorbent after washing, filter, dry, grind and sieving.Control group mould hyphaball powder sorbent adsorptive capacity is 5.7mg/g.
This test test group can simplify absorbent preparation step, improves heavy metal adsorption amount while cost-saving.
Test 2, this test test group utilize the method that mould spores adsorbs heavy metal in waste water to prepare according to following steps:
One, mould spores is inoculated on improvement Martin substratum, cultivates 7 days under the condition of 30 DEG C, then collect the mould spores that mould aerial hyphae comes off naturally;
Two, the mould spores that step one is collected being added to copper content is in the waste water of 30mg/L, then in temperature is to adsorb under the condition of 30 DEG C, absorption 2h, and stirring velocity is 0rmp, namely completes; The wherein concrete strain name of mould and Latin; The mass volume ratio of mould spores and copper-containing wastewater is 1:1000g/mL.
The adsorptive capacity of test group spore is 24.5mg/g.
Mould in test 1 ~ 2 is the aspergillus niger of bacterial strain numbers 98003, buys from Harbin Chun Rui Science and Technology Development Co., Ltd..
Claims (7)
1. utilize mould spores to adsorb a method for heavy metal in waste water, it is characterized in that utilizing the method that mould spores adsorbs heavy metal in waste water to prepare according to following steps:
One, mould spores is inoculated on solid medium, cultivates under the condition of 29 ~ 31 DEG C, then collect the mould spores that mould aerial hyphae comes off naturally;
Two, being added in the waste water containing heavy metal by the mould spores that step one is collected, is then stir under the condition of 10 ~ 40 DEG C in temperature, and absorption 1.5 ~ 2.5h, namely completes; Mould spores is 1:(900 ~ 1100 with the mass volume ratio of the waste water containing heavy metal) g/mL.
2. a kind of mould spores that utilizes according to claim 1 adsorbs the method for heavy metal in waste water, it is characterized in that described mould is the aspergillus niger of bacterial strain numbers 98003.
3. a kind of method utilizing mould spores to adsorb heavy metal in waste water according to claim 1, is characterized in that the solid medium described in step one is for improvement Martin substratum, PDA substratum or dry rice straw substratum.
4. a kind of method utilizing mould spores to adsorb heavy metal in waste water according to claim 1, is characterized in that the heavy metal described in step 2 is copper.
5. a kind of method utilizing mould spores to adsorb heavy metal in waste water according to claim 1, is characterized in that stirring under temperature is the condition of 30 DEG C described in step 2.
6. a kind of method utilizing mould spores to adsorb heavy metal in waste water according to claim 1, is characterized in that the stirring velocity described in step 2 is 0 ~ 300rmp.
7. a kind of method utilizing mould spores to adsorb heavy metal in waste water according to claim 1, is characterized in that the adsorption time described in step 2 is 2h.
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| CN201510226403.2A CN104787902A (en) | 2015-05-06 | 2015-05-06 | Method for adsorbing heavy metal in wastewater by using mold spore |
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| CN201510226403.2A CN104787902A (en) | 2015-05-06 | 2015-05-06 | Method for adsorbing heavy metal in wastewater by using mold spore |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4732681A (en) * | 1985-05-02 | 1988-03-22 | Ramot University Authority For Applied Research And Industrial Development Ltd. | Removal of contaminants |
| CN103525870A (en) * | 2013-10-23 | 2014-01-22 | 嘉兴学院 | Microbial flocculant as well as preparation method and application thereof |
| CN104261564A (en) * | 2014-10-14 | 2015-01-07 | 南华大学 | Method for repairing low-concentration uranium polluted water by utilizing syngonium podophyllum-aspergillus niger symbiotic system |
| CN104386832A (en) * | 2014-12-05 | 2015-03-04 | 河南省科学院生物研究所有限责任公司 | Method for processing leaded wastewater by using microbial bacteria |
-
2015
- 2015-05-06 CN CN201510226403.2A patent/CN104787902A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4732681A (en) * | 1985-05-02 | 1988-03-22 | Ramot University Authority For Applied Research And Industrial Development Ltd. | Removal of contaminants |
| CN103525870A (en) * | 2013-10-23 | 2014-01-22 | 嘉兴学院 | Microbial flocculant as well as preparation method and application thereof |
| CN104261564A (en) * | 2014-10-14 | 2015-01-07 | 南华大学 | Method for repairing low-concentration uranium polluted water by utilizing syngonium podophyllum-aspergillus niger symbiotic system |
| CN104386832A (en) * | 2014-12-05 | 2015-03-04 | 河南省科学院生物研究所有限责任公司 | Method for processing leaded wastewater by using microbial bacteria |
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Application publication date: 20150722 |
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