CN104783806A - Method for displaying latent fingerprints through fluorescent copper sol - Google Patents
Method for displaying latent fingerprints through fluorescent copper sol Download PDFInfo
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- CN104783806A CN104783806A CN201510220520.8A CN201510220520A CN104783806A CN 104783806 A CN104783806 A CN 104783806A CN 201510220520 A CN201510220520 A CN 201510220520A CN 104783806 A CN104783806 A CN 104783806A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/117—Identification of persons
- A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
- A61B5/1172—Identification of persons based on the shapes or appearances of their bodies or parts thereof using fingerprinting
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- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
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Abstract
The invention provides a method for displaying latent fingerprints through fluorescent copper sol. The copper sol with the high fluorescent performance is used as a displaying material for displaying the latent fingerprints. The copper sol with the fluorescent emitting function is prepared with the ascorbic acid as a reducing agent, bovine serum albumin as a protective agent and cysteine as a stabilizer. The fluorescent copper sol can serve as a displaying reagent for displaying the latent fingerprints. Under an ultraviolet lamp, the copper sol can display the latent fingerprints into fluorescent stripes, the display result is clear, and the materials are non-toxic, low in cost and suitable for displaying the latent fingerprints on the surfaces of various objects.
Description
Technical field
the invention belongs to detection technique field, being specifically related to fluorescence copper colloidal sol for manifesting the method for laten fingerprints.
Background technology
It is variant that fingerprint has people, throughout one's life the feature of constant and tactile thing trace.Science correctly finds, extract, manifest and identify fingerprint for carrying out investigation, punishing crime has important practice effect; Fingerprint identification is one of the most reliable method of carrying out individual identification, is used for investigating, disclosing and confirm crime in forensic science; In the withering and look into and process of accident, the victim of some accidents or natural disaster or died, because of the condition of the injury or other reasons be with the naked eye beyond recognition time, extract their fingerprint, often can provide definite identification; In civil activity. by the input of the finger print data of individual identity card, the credit card and books borrower's card, can be used to identify that whether registration person is same with holder; Same application also can in security protection, safety check and commercial affairs, as automatic door lock unlatching, go out the aspect such as population management and control of stamping; In clinical medicine, fingerprint can be examination and diagnoses the illness provides information and foundation; Along with the universal of computer technology and the application of automatic system of fingerprint recognition, fingerprint technique is about to obtain applying more and more widely in the life of people.
Be applied to the method that fingerprint on site manifests at present to mainly contain: optics appearance method, physics appearance method and chemical appearance method.The common weakness of these methods is that sensitivity is low, produces interference after the method application wherein had to the sensitivity of DNA tests and result.Wherein most widely used method is powder method, but very large to the physical impairment of professional and technical personnel, and the deadly defect of this method can not manifest outmoded fingerprint.Fluorescent appear method has special advantage, the laten fingerprints that some differentiate unconspicuous background surface can be manifested, reasons for its use fluorescence is very large to the interference of the luminescence generated by light Bio-imprinting identification of routine simultaneously for the photoluminescent techniques of current use, and difficulty reaches the requirement of expection.Therefore, the research and development of Non-toxic and high sensitivity live biometric trace appearing technique have become the emphasis of forensic science research and the striving direction of countries in the world Forensic Science personnel.
Metal nanoparticle has successfully been applied in the diagnosis and treatment of various nano biological sensor and disease, huge application prospect is had in biomolecule detection and sensing, metal nanometer cluster also receives much concern simultaneously, its reason is unique physics, electrically, and the application of optical property in catalysis very promising.Metal nanoparticle preparation is simple, uniform particle sizes, stable chemical nature, affinity strong, good biocompatibility, be easy to the advantages such as the fixing modification of biomolecule, can improve the sensitivity that Bio-imprinting manifests.When utilizing the laten fingerprints of gold nano grain to the object such as paper, sheet glass surface, row manifests and shows very high sensitivity, but because gold nano grain is that cost is high as manifesting the obvious problem of reagent, the too high problem of funds will be there is to carrying out large-scale use.Manifest laten fingerprints therefore, it is possible to quick, highly sensitive, expense can be reduced again is the problem that researcher needs to solve.We are based on this demand, having invented a kind of copper colloidal sol with photoluminescent property, can manifest laten fingerprints as manifesting reagent, manifest that process is fast and material cost is very low, are suitable for large-scale application.
Summary of the invention
The object of this invention is to provide a kind of method utilizing copper colloidal sol to manifest laten fingerprints, with there is fluorescent both matter copper colloidal sol as manifesting materials exhibit laten fingerprints.
Preparation has hyperfluorescence character copper colloidal sol and is manifesting the application in laten fingerprints, and detailed process is as follows:
1, the preparation of copper colloidal sol:
Copper colloidal sol is prepared by two-step method:
(1) preparation of precursor liquid: at room temperature, the precursor solution of copper ion has been prepared by reduction copper nitrate, utilize ascorbic acid as reducing agent, under magnetic stirring, copper nitrate aqueous solution (0.1-0.5 M) is dropwise added excessive ascorbic acid AA solution (0.1-0.5 M), the volume ratio of two kinds of solution can within the scope of 1:1-1:10, and at room temperature sustained response 1-2 hour.
(2) add protective agent and form fluorescence colloidal sol:
The copper ion precursor solution prepared is added in isopyknic protective agent bovine serum albumin (BSA) solution (0.05 M-0.1 M) above; at room temperature stir 3-5 hour; obtain a kind of liquid of colloidal sol shape; the unreacted material of centrifugal removing; be re-dispersed in water and be still molten colloidal solution, under the condition that ultraviolet source excites, send blue-fluorescence.
The copper ion precursor solution prepared is added in isopyknic protective agent cysteine (L-cys) solution (0.05 M-0.1 M) above; at room temperature stir 3-5 hour; obtain white precipitate; the unreacted material of centrifugal removing; white precipitate presents the transmitting of HONGGUANG under ultraviolet source; be re-dispersed in water and become molten colloidal solution, under the condition that ultraviolet source excites, send red fluorescence.
2, copper colloidal sol is used for manifesting of laten fingerprints:
After the copper colloidal sol of preparation dilution 1-3 times, the copper colloidal sol that takes a morsel is sprayed on rapidly the surface that may there is perspiration or blood laten fingerprints sample, colloidal sol slowly solidifies on the solid of sample surfaces, copper colloidal sol is at glass, silicon chips etc. define the sol pellicle of one deck thickness on the surface, under ultraviolet source, thin film sends fluorescence, the fingerprint ridge of the fingerprint sample below thin film clearly can be presented, due to, the colloidal sol with fluorescence be set on sample is transparent, under uviol lamp, the streakline part of fingerprint can reflect by transparent fluorescence well, thus reach the object of fluorescent appear laten fingerprints, now, capable of taking pictures carrying out leaves and takes finger print data, because thin film is more crisp, edge part branch is the embrittlement because dried-up time is long automatically, therefore, data need be left and taken as early as possible, although the edge of sol pellicle easily splits, but do not affect long-time preservation, fingerprint sample after manifesting can retain more than three months.
accompanying drawing illustrates:
The fluorescence spectrum figure of accompanying drawing 1, fluorescence copper colloidal sol;
Accompanying drawing 2, fluorescence copper colloidal sol are at daylight lamp and uviol lamp picture;
The laten fingerprints picture that accompanying drawing 3, fluorescence copper colloidal sol manifest.
Tool of the present invention has the following advantages:
1, sensitive height, quick, easy to operate.
2, synthesize, testing process is without any side effects.
3, do not destroy the potential information of laten fingerprints, do not affect the DNA qualification of laten fingerprints.
4, copper salt is cheap, and material is easily produced.
embodiment 1:
The preparation of copper colloidal sol:
(1) preparation of precursor liquid: at room temperature, the precursor solution of copper ion has been prepared by reduction copper nitrate, utilize ascorbic acid as reducing agent, under magnetic stirring, copper nitrate aqueous solution (0.2 M) is dropwise added in excessive ascorbic acid AA solution (0.2 M), the 1:1 mixing by volume of two kinds of solution usage, and at room temperature sustained response 1 hour.
(2) add protective agent and form fluorescence colloidal sol:
The copper ion precursor solution prepared is added in isopyknic protective agent BSA solution (0.05 M-0.1 M) above; remain on stirred at ambient temperature 3 hours; obtain a kind of liquid of colloidal sol shape; the unreacted material of centrifugal removing; be re-dispersed in water and be still molten colloidal solution, under the condition that ultraviolet source excites, send blue-fluorescence.
embodiment 2:
(1) preparation of precursor liquid: at room temperature, has prepared the precursor solution of copper ion by reduction copper nitrate, uses ascorbic acid as reducing agent.Under magnetic stirring, drip excessive ascorbic acid AA solution (0.1-0.5 M) toward copper nitrate aqueous solution (0.1-0.5 M), the volume ratio of two kinds of solution can within the scope of 1:1-1:10, and reacts under indoor temperature and stir the mixture 2 hours.
(2) add protective agent and form fluorescence colloidal sol:
The copper ion precursor solution prepared is added in isopyknic protective agent cysteine solution (0.05 M-0.1 M) above; remain on stirred at ambient temperature 4.5 hours; obtain white precipitate; the unreacted material of centrifugal removing; white precipitate presents the transmitting of HONGGUANG under ultraviolet source; be re-dispersed in water and become molten colloidal solution, under the condition that ultraviolet source excites, send red fluorescence.
embodiment 3:
Copper colloidal sol is used for manifesting of laten fingerprints:
After the copper colloidal sol protected by the BSA of preparation dilutes 2 times, take a morsel and drop in rapidly the surface of perspiration laten fingerprints sample, colloidal sol slowly solidifies on the solid of sample surfaces, copper colloidal sol is at glass, silicon chips etc. define the thin film of one deck thickness on the surface, under ultraviolet source, thin film sends blue fluorescence, the fingerprint ridge of the fingerprint sample below thin film clearly can be presented, due to, the colloidal sol with fluorescence dropped on sample is transparent, under uviol lamp, the streakline part of fingerprint can reflect by transparent blue-fluorescence well, thus reach the object of fluorescent appear laten fingerprints, now, capable of taking pictures carrying out leaves and takes finger print data.
Claims (3)
1. a preparation method for fluorescence copper colloidal sol, is characterized in that concrete steps are as follows:
(1) preparation of precursor liquid: at room temperature, the precursor solution of copper ion has been prepared by reduction copper nitrate, use ascorbic acid as reducing agent, under magnetic stirring, drip at excessive ascorbic acid AA solution 0.1-0.5 M toward copper nitrate aqueous solution 0.1-0.5 M, the volume ratio of two kinds of solution can within the scope of 1:1-1:10, and room temperature reacts 1-2 hour under magnetic agitation condition;
(2) add protective agent and form fluorescence colloidal sol: the copper ion precursor solution prepared is added in isopyknic protective agent bovine serum albumin or cysteine solution 0.05 M-0.1 M above; at room temperature Keep agitation 3-5 hour; obtain a kind of liquid of colloidal sol shape; the unreacted material of centrifugal removing; be re-dispersed in water and be still molten colloidal solution, under the condition that ultraviolet source excites, send fluorescence.
2. the application of a fluorescence copper colloidal sol in developing latent finger prints.
3. a fluorescence copper colloidal sol is used for the method for developing latent finger prints, it is characterized in that: when copper colloidal sol is used for manifesting of laten fingerprints, after the copper colloidal sol of preparation dilution 1-3 times, the copper colloidal sol that takes a morsel is sprayed on rapidly the surface that there is perspiration or blood laten fingerprints sample, colloidal sol slowly solidifies on the solid of sample surfaces, copper colloidal sol defines the sol pellicle of one deck thickness on glass, silicon chip surface, under ultraviolet source, thin film sends blue fluorescence, the fingerprint ridge of the fingerprint sample below thin film clearly can be presented, reach the object of fluorescent appear laten fingerprints.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510220520.8A CN104783806B (en) | 2015-05-05 | 2015-05-05 | A kind of method that fluorescence copper colloidal sol is used for developing latent finger printss |
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| CN201510220520.8A CN104783806B (en) | 2015-05-05 | 2015-05-05 | A kind of method that fluorescence copper colloidal sol is used for developing latent finger printss |
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| CN104783806B CN104783806B (en) | 2017-06-30 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109557066A (en) * | 2019-01-25 | 2019-04-02 | 西南政法大学 | Copper nano-cluster Sweat latent fingerprint shows preparation method of reagent thereof and Sweat latent fingerprint process for show |
| CN110464361A (en) * | 2019-08-06 | 2019-11-19 | 中南民族大学 | A kind of method that enzymatic reaction system shows for latent fingerprint |
Citations (5)
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| CN101697873A (en) * | 2009-09-30 | 2010-04-28 | 中国人民公安大学 | Method for developing latent fingerprints by adopting nano zinc oxide |
| WO2010109222A1 (en) * | 2009-03-24 | 2010-09-30 | University Of Leicester | Method of visualising a (finger) print on a surface |
| CN103431867A (en) * | 2013-08-20 | 2013-12-11 | 北京科技大学 | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein |
| CN103784149A (en) * | 2014-03-05 | 2014-05-14 | 北京科技大学 | Method for developing latent fingerprints through multi-metal deposition technology |
| CN104337522A (en) * | 2014-11-24 | 2015-02-11 | 中国刑事警察学院 | Method for displaying latent fingerprints by adopting fluorescent dye-modified nano particles |
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2015
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010109222A1 (en) * | 2009-03-24 | 2010-09-30 | University Of Leicester | Method of visualising a (finger) print on a surface |
| CN101697873A (en) * | 2009-09-30 | 2010-04-28 | 中国人民公安大学 | Method for developing latent fingerprints by adopting nano zinc oxide |
| CN103431867A (en) * | 2013-08-20 | 2013-12-11 | 北京科技大学 | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein |
| CN103784149A (en) * | 2014-03-05 | 2014-05-14 | 北京科技大学 | Method for developing latent fingerprints through multi-metal deposition technology |
| CN104337522A (en) * | 2014-11-24 | 2015-02-11 | 中国刑事警察学院 | Method for displaying latent fingerprints by adopting fluorescent dye-modified nano particles |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109557066A (en) * | 2019-01-25 | 2019-04-02 | 西南政法大学 | Copper nano-cluster Sweat latent fingerprint shows preparation method of reagent thereof and Sweat latent fingerprint process for show |
| CN109557066B (en) * | 2019-01-25 | 2021-06-15 | 西南政法大学 | Preparation method of copper nano-cluster sweat latent fingerprint developing reagent and sweat latent fingerprint developing method |
| CN110464361A (en) * | 2019-08-06 | 2019-11-19 | 中南民族大学 | A kind of method that enzymatic reaction system shows for latent fingerprint |
| CN110464361B (en) * | 2019-08-06 | 2022-04-05 | 中南民族大学 | Method for potential fingerprint display by enzymatic reaction system |
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Inventor after: Chai Fang Inventor after: Wang Xu Inventor after: Lin Shuangyan Inventor after: Zhang Qi Inventor after: Ren Guojuan Inventor before: Chai Fang Inventor before: Wang Xu Inventor before: Zhang Qi Inventor before: Ren Guojuan |
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Granted publication date: 20170630 Termination date: 20180505 |