CN104783806B - A kind of method that fluorescence copper colloidal sol is used for developing latent finger printss - Google Patents
A kind of method that fluorescence copper colloidal sol is used for developing latent finger printss Download PDFInfo
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- CN104783806B CN104783806B CN201510220520.8A CN201510220520A CN104783806B CN 104783806 B CN104783806 B CN 104783806B CN 201510220520 A CN201510220520 A CN 201510220520A CN 104783806 B CN104783806 B CN 104783806B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/117—Identification of persons
- A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
- A61B5/1172—Identification of persons based on the shapes or appearances of their bodies or parts thereof using fingerprinting
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Abstract
The present invention provides a kind of method that utilization copper colloidal sol manifests latent fingerprint, with the copper colloidal sol with fluorescent both matter as manifesting the latent fingerprint of materials exhibit.By the use of ascorbic acid as reducing agent; by the use of bovine serum albumin(BSA) and cysteine as protective agent and stabilizer; it is prepared for the copper colloidal sol with fluorescent emission; fluorescence copper colloidal sol can manifest latent fingerprint as reagent is manifested; under uviol lamp, developing latent finger printss fluorescence striped can be gone out with copper colloidal sol, manifest result clear; material non-toxic, low cost, it is adaptable to the developing latent finger printss on various object surfaces.
Description
Technical field
The invention belongs to detection technique field, and in particular to fluorescence copper colloidal sol is used for the method for manifesting latent fingerprint.
Background technology
Fingerprint has people variant, throughout one's life the characteristics of constant and tactile thing trace.Science correctly find, extract, manifesting and
Identification fingerprint is for carrying out investigation, punish crime acts on important practice；Fingerprint identification carries out individual identification
One of most reliable method, is used for investigating, disclose and confirming crime in forensic science；In the withering and look into and process of accident, one
Victim or the died of a little accidents or natural calamity, when gesture or other reasonses are with the naked eye beyond recognition due to wound, extract theirs
Fingerprint, tends to provide definite identification；Personal finger print data is input into identity card, credit card and figure by civil activity
In book borrower's card, can be used to recognize whether registration person is same with holder；Same application can also be in security protection, safety check and business
In business, such as the unlatching of automatic door lock, go out population management and control of stamping in terms of；Fingerprint can be examination and examine in clinical medicine
Disconnected disease provides information and foundation；With the popularization and the application of automatic system of fingerprint recognition of computer technology, fingerprint technique is
To be increasingly widely applied in the life of people.
Being applied to the method that fingerprint on site manifests at present mainly has：Optics appearance method, physics appearance method and chemical appearance method.
The common weakness of these methods is low sensitivity, wherein the sensitivity of DNA tests and result are produced after some method applications doing
Disturb.Wherein most widely used method is powder method, but very big to the physical impairment of professional and technical personnel, and this method
Deadly defect is that outmoded fingerprint can not be manifested.Fluorescence appearance method has special advantage, it is possible to visualise some are differentiated not
The latent fingerprint of obvious background surface, the photoluminescent techniques for using at present are while reasons for its use fluorescence is to conventional photic hair
The interference of photo-biological print identification is very big, and difficulty reaches expected requirement.Therefore, Non-toxic and high sensitivity live biometric trace are aobvious
The research and development of existing technology have turned into the emphasis of forensic science research and the striving direction of countries in the world criminal technique personnel.
Metal nanoparticle has been applied successfully in the diagnosis and treatment of various nano biological sensors and disease, in biology point
There is huge application prospect in son detection and sensing, while metal nanometer cluster also receives much concern, its reason is unique thing
Reason, electrically, and application of the optical property in catalysis is very promising.Metal nanoparticle prepares simple, uniform particle sizes, chemically
Matter stabilization, affinity strong, good biocompatibility, the advantages of be easy to biomolecule fixation and modify, can improve what Bio-imprinting manifested
Sensitivity.Row manifests and shows sensitivity very high during using gold nano grain to the latent fingerprint on the object such as paper, sheet glass surface,
But as the obvious problem of reagent is manifested it is cost high due to gold nano grain, will exists to carrying out large-scale use
The too high problem of funds.Manifest latent fingerprint therefore, it is possible to quick, highly sensitive, can be again that researcher needs solution by expense reduction
Problem certainly.We are based on this demand, have invented a kind of copper colloidal sol with photoluminescent property, can manifest as reagent is manifested
Latent fingerprint, manifests process soon and material cost is very low, is suitable to large-scale application.
The content of the invention
It is an object of the invention to provide a kind of method that utilization copper colloidal sol manifests latent fingerprint, with molten with fluorescent both matter copper
Glue is used as manifesting the latent fingerprint of materials exhibit.
Preparation has hyperfluorescence property copper colloidal sol and the application in latent fingerprint is manifested, and detailed process is as follows：
1st, the preparation of copper colloidal sol：
Copper colloidal sol is prepared by two-step method：
（1）The preparation of precursor liquid：At room temperature, the precursor solution of copper ion is prepared for by reducing copper nitrate, is utilized
Ascorbic acid as reducing agent, under magnetic stirring, by copper nitrate aqueous solution（0.1-0.5 M）It is added dropwise over excessive Vitamin C
Sour AA solution（0.1 -0.5 M ）, two kinds of volume ratios of solution can be 1：1-1：In the range of 10, and sustained response 1- at room temperature
2 hours.
（2）Protective agent is added to form fluorescence colloidal sol：
Copper ion precursor solution prepared above is added to isometric protective agent bovine serum albumin(BSA)（BSA）Solution
（0.05 M-0.1 M）In, it is stirred at room temperature 3-5 hours, a kind of liquid of colloidal sol shape is obtained, it is centrifuged off unreacted thing
Matter, it is still molten colloidal solution to be re-dispersed into water, and blue-fluorescence is sent under conditions of ultraviolet source is excited.
Copper ion precursor solution prepared above is added to isometric protective agent cysteine（L-cys）Solution
（0.05 M-0.1 M）In, it is stirred at room temperature 3-5 hours, white precipitate is obtained, unreacted material is centrifuged off, white is heavy
Form sediment and the transmitting of feux rouges is presented under ultraviolet source, being re-dispersed into turns into molten colloidal solution in water, in the bar that ultraviolet source is excited
Red fluorescence is sent under part.
2nd, copper colloidal sol manifesting for fingerprint of diving：
After the copper colloidal sol that will be prepared dilutes 1-3 times, take rapid being sprayed on of a small amount of copper colloidal sol and there may be sweat or the latent finger of blood
The surface of grain pattern product, colloidal sol slowly solidifies on the solid of sample surfaces, and copper colloidal sol forms one on the surfaces such as glass, silicon chip
The sticky sol pellicle of layer, under ultraviolet source, film sends fluorescence, can be clear by the fingerprint ridge of the fingerprint sample below film
Show clearly, due to, the colloidal sol with fluorescence being set on sample is transparent, under uviol lamp, transparent fluorescence
The streakline part of fingerprint can be reflected well, so as to reach the purpose that fluorescence manifests latent fingerprint, now, it is capable of taking pictures enter
Row leaves and takes finger print data, because film is more crisp, marginal portion can from reason dried-up time is long and embrittlement, therefore, need to leave and take as early as possible
Data, although the edge of sol pellicle easily splits, have no effect on and preserve for a long time, and the fingerprint sample after manifesting can retain three months
More than.
Brief description of the drawings：
The fluorescence spectra of accompanying drawing 1, fluorescence copper colloidal sol；
Accompanying drawing 2, fluorescence copper colloidal sol are in fluorescent lamp and uviol lamp picture；
The latent fingerprint picture that accompanying drawing 3, fluorescence copper colloidal sol manifest.
The invention has the advantages that：
1st, it is sensitive high, quick, easy to operate.
2nd, synthesis, detection process are without any side effects.
3rd, the potential information of latent fingerprint is not destroyed, does not influence the DNA of latent fingerprint to identify.
4th, copper salt is cheap, and material is easily produced.
Embodiment 1：
The preparation of copper colloidal sol：
（1）The preparation of precursor liquid：At room temperature, the precursor solution of copper ion is prepared for by reducing copper nitrate, is utilized
Ascorbic acid as reducing agent, under magnetic stirring, by copper nitrate aqueous solution（0.2 M）Dropwise it is added in excessive ascorbic acid AA
Solution（0.2 M ）, two kinds of solution usages by volume 1：1 mixing, and sustained response 1 hour at room temperature.
（2）Protective agent is added to form fluorescence colloidal sol：
Copper ion precursor solution prepared above is added to isometric protective agent BSA solution（0.05 M-0.1 M）
In, stirring 3 hours is kept at room temperature, a kind of liquid of colloidal sol shape is obtained, unreacted material is centrifuged off, it is re-dispersed into
It is still molten colloidal solution in water, blue-fluorescence is sent under conditions of ultraviolet source is excited.
Embodiment 2：
（1）The preparation of precursor liquid：At room temperature, the precursor solution of copper ion is prepared for by reducing copper nitrate, is used
Ascorbic acid is used as reducing agent.Under magnetic stirring, toward copper nitrate aqueous solution（0.1-0.5 M）Excessive ascorbic acid AA is added dropwise
Solution（0.1 -0.5 M ）, two kinds of volume ratios of solution can be 1：1-1：In the range of 10, and indoors at a temperature of to react stirring mixed
Compound 2 hours.
（2）Protective agent is added to form fluorescence colloidal sol：
Copper ion precursor solution prepared above is added to isometric protective agent cysteine solution（0.05 M-
0.1 M）In, stirring 4.5 hours is kept at room temperature, white precipitate is obtained, unreacted material is centrifuged off, white precipitate exists
The transmitting of feux rouges is presented under ultraviolet source, being re-dispersed into turns into molten colloidal solution in water, under conditions of ultraviolet source is excited
Send red fluorescence.
Embodiment 3：
Copper colloidal sol manifests for fingerprint of diving：
After the copper colloidal sol of the BSA protections that will be prepared dilutes 2 times, a small amount of surface for dropping in the latent fingerprint sample of sweat rapidly is taken,
Colloidal sol slowly solidifies on the solid of sample surfaces, and copper colloidal sol forms one layer of sticky film on the surfaces such as glass, silicon chip,
Under ultraviolet source, film sends the fluorescence of blueness, can clearly show the fingerprint ridge of the fingerprint sample below film
Come, because it is transparent to drop in the colloidal sol with fluorescence on sample, and under uviol lamp, transparent blue-fluorescence is by fingerprint
Streakline part can reflect well, now, capable of taking pictures to carry out leaving and taking finger so as to reach the purpose that fluorescence manifests latent fingerprint
Line data.
Claims (3)
1. a kind of preparation method of fluorescence copper colloidal sol, it is characterized in that comprising the following steps that：
（1）The preparation of precursor solution：At room temperature, the precursor solution of copper ion is prepared for by reducing copper nitrate, is used
Used as reducing agent, under magnetic stirring, the copper nitrate aqueous solution toward 0.1-0.5 M is added dropwise 0.1-0.5 excessive M to ascorbic acid
Ascorbic acid AA solution, two kinds of volume ratios of solution of copper nitrate and ascorbic acid are 1：1-1 ：In the range of 10, in room temperature and
Reacted 1-2 hours under the conditions of magnetic agitation；
（2）Protective agent is added to form fluorescence colloidal sol：Copper ion precursor solution prepared above is added to isometric protection
In the bovine serum albumin(BSA) of the M-0.1 M of agent 0.05 or the cysteine solution of 0.05 M-0.1 M, it is continuously stirred at room temperature
3-5 hours, obtain a kind of liquid of colloidal sol shape, be centrifuged off unreacted material, white precipitate be re-dispersed into water in be still
Molten colloidal solution, fluorescence is sent under conditions of ultraviolet source is excited.
2. application of the fluorescence copper colloidal sol that prepared by the method described in claim 1 in developing latent finger printss.
3. the method that the fluorescence copper colloidal sol that prepared by the method described in claim 1 is used for developing latent finger printss, it is characterized in that：Copper colloidal sol
For fingerprint of diving when manifesting, will prepare copper colloidal sol dilute 1-3 times after, take a small amount of copper colloidal sol rapidly be sprayed on exist sweat or
The surface of the latent fingerprint sample of blood, colloidal sol slowly solidifies on the solid of sample surfaces, and described sample is glass or silicon chip, copper
Colloidal sol forms one layer of sticky sol pellicle on glass or silicon chip surface, and under ultraviolet source, film sends blue or red
The fluorescence of light, can clearly show the fingerprint ridge of the fingerprint sample below film, reach fluorescence and manifest latent fingerprint
Purpose.
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| CN201510220520.8A CN104783806B (en) | 2015-05-05 | 2015-05-05 | A kind of method that fluorescence copper colloidal sol is used for developing latent finger printss |
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| CN201510220520.8A CN104783806B (en) | 2015-05-05 | 2015-05-05 | A kind of method that fluorescence copper colloidal sol is used for developing latent finger printss |
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| CN104783806B true CN104783806B (en) | 2017-06-30 |
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| CN109557066B (en) * | 2019-01-25 | 2021-06-15 | 西南政法大学 | Preparation method of copper nano-cluster sweat latent fingerprint developing reagent and sweat latent fingerprint developing method |
| CN110464361B (en) * | 2019-08-06 | 2022-04-05 | 中南民族大学 | Method for potential fingerprint display by enzymatic reaction system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101697873A (en) * | 2009-09-30 | 2010-04-28 | 中国人民公安大学 | Method for developing latent fingerprints by adopting nano zinc oxide |
| CN103431867A (en) * | 2013-08-20 | 2013-12-11 | 北京科技大学 | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein |
| CN103784149A (en) * | 2014-03-05 | 2014-05-14 | 北京科技大学 | Method for developing latent fingerprints through multi-metal deposition technology |
| CN104337522A (en) * | 2014-11-24 | 2015-02-11 | 中国刑事警察学院 | Method for displaying latent fingerprints by adopting fluorescent dye-modified nano particles |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2471446A (en) * | 2009-03-24 | 2011-01-05 | Univ Leicester | Visualising a print on a surface |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101697873A (en) * | 2009-09-30 | 2010-04-28 | 中国人民公安大学 | Method for developing latent fingerprints by adopting nano zinc oxide |
| CN103431867A (en) * | 2013-08-20 | 2013-12-11 | 北京科技大学 | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein |
| CN103784149A (en) * | 2014-03-05 | 2014-05-14 | 北京科技大学 | Method for developing latent fingerprints through multi-metal deposition technology |
| CN104337522A (en) * | 2014-11-24 | 2015-02-11 | 中国刑事警察学院 | Method for displaying latent fingerprints by adopting fluorescent dye-modified nano particles |
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Inventor after: Chai Fang Inventor after: Wang Xu Inventor after: Lin Shuangyan Inventor after: Zhang Qi Inventor after: Ren Guojuan Inventor before: Chai Fang Inventor before: Wang Xu Inventor before: Zhang Qi Inventor before: Ren Guojuan |
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